MD16G1039400.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001240
Physical Location & Seq
Forward (+)
2785256 .. 2785606
351 bp
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UTR
Exon/CDS
Intron
MD16G1039400.v1.1.491

Sequence Viewer

Length: 351 bp
ATGAATGAAGTGGCAGCCATTAGTAGAACTTCCCGTGTCAACATCGTCTCCTTGTTGGGCTACTGTTTTGAGGGTTCGAAGAGAGCTCTCATCTATGAATTCATGCCTAATGGATCGCTTGAGAAATTCATATTTGATGCAAGTACTCCCAAGAAAGATCATCATCACTTGGGATGGGAAACTGTATATTTAATTTCACTCGGTATTCCTCGAGGATTGGAGTACTTACATCGTGGTTGCAAAACACAAATTTTGCATTTCGACATCAAGCCTCACAACATGCTTCTCGATGAAAACTTTACCCCAAAAATCTCGGATTTTGGCCTTGCCACACTATGCAATAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

117

Amino Acids

13.27

Weight (kDa)

8.48

Isoelectric Point (pI)

17.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 113 3e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 2 - 115 3.7e-19 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 210
AclWI GGATC 1 cut(s) 121
AcsI RAATTY 3 cut(s) 98, 125, 249
AfaI GTAC 2 cut(s) 145, 224
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
Alw21I GWGCWC 1 cut(s) 88
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 1 cut(s) 121
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 3 cut(s) 98, 125, 249
AsuII TTCGAA 1 cut(s) 77
AvaI CYCGRG 1 cut(s) 210
BanII GRGCYC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 26
BccI CCATC 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 52
BisI GCNGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 16
BmcAI AGTACT 2 cut(s) 145, 224
BmeT110I CYCGRG 1 cut(s) 210
BmsI GCATC 1 cut(s) 127
Bpu14I TTCGAA 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bse8I GATNNNNATC 1 cut(s) 162
BseGI GGATG 1 cut(s) 179
BseJI GATNNNNATC 1 cut(s) 162
BseXI GCAGC 1 cut(s) 26
BshFI GGCC 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsnI GGCC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 210
Bsp119I TTCGAA 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 88
Bsp143I GATC 2 cut(s) 113, 157
BspANI GGCC 1 cut(s) 324
BspPI GGATC 1 cut(s) 121
BspT104I TTCGAA 1 cut(s) 77
BssMI GATC 2 cut(s) 113, 157
Bst4CI ACNGT 2 cut(s) 65, 184
Bst6I CTCTTC 1 cut(s) 74
BstBI TTCGAA 1 cut(s) 77
BstF5I GGATG 1 cut(s) 179
BstKTI GATC 2 cut(s) 116, 160
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 2 cut(s) 113, 157
BstNSI RCATGY 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 26
BsuRI GGCC 1 cut(s) 324
BtsCI GGATG 1 cut(s) 179
Csp6I GTAC 2 cut(s) 144, 223
CviAII CATG 2 cut(s) 103, 280
CviJI RGCY 5 cut(s) 17, 60, 86, 271, 324
CviKI_1 RGCY 5 cut(s) 17, 60, 86, 271, 324
CviQI GTAC 2 cut(s) 144, 223
DpnI GATC 2 cut(s) 115, 159
DpnII GATC 2 cut(s) 113, 157
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 2 cut(s) 106, 283
FaiI YATR 6 cut(s) 96, 104, 131, 187, 281, 337
FatI CATG 2 cut(s) 102, 279
Fnu4HI GCNGC 1 cut(s) 15
FokI GGATG 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 1 cut(s) 15
GluI GCNGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 324
Hin1II CATG 2 cut(s) 106, 283
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 65, 184
HpyCH4V TGCA 4 cut(s) 140, 240, 256, 339
Hsp92II CATG 2 cut(s) 106, 283
Kzo9I GATC 2 cut(s) 113, 157
Lsp1109I GCAGC 1 cut(s) 26
LweI GCATC 1 cut(s) 127
MalI GATC 2 cut(s) 115, 159
MboI GATC 2 cut(s) 113, 157
MboII GAAGA 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 88
MluCI AATT 4 cut(s) 98, 125, 192, 249
MnlI CCTC 4 cut(s) 64, 206, 219, 282
MseI TTAA 1 cut(s) 191
NdeII GATC 2 cut(s) 113, 157
NlaIII CATG 2 cut(s) 106, 283
NspI RCATGY 1 cut(s) 283
NspV TTCGAA 1 cut(s) 77
PaeR7I CTCGAG 1 cut(s) 210
PkrI GCNGC 1 cut(s) 16
Psp124BI GAGCTC 1 cut(s) 88
PspXI VCTCGAGB 1 cut(s) 210
RsaI GTAC 2 cut(s) 145, 224
RsaNI GTAC 2 cut(s) 144, 223
SacI GAGCTC 1 cut(s) 88
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 2 cut(s) 113, 157
ScaI AGTACT 2 cut(s) 145, 224
SduI GDGCHC 1 cut(s) 88
SetI ASST 1 cut(s) 88
SfaNI GCATC 1 cut(s) 127
Sfr274I CTCGAG 1 cut(s) 210
SfuI TTCGAA 1 cut(s) 77
SlaI CTCGAG 1 cut(s) 210
SmlI CTYRAG 2 cut(s) 119, 210
SmoI CTYRAG 2 cut(s) 119, 210
Sse9I AATT 4 cut(s) 98, 125, 192, 249
SstI GAGCTC 1 cut(s) 88
TaaI ACNGT 2 cut(s) 65, 184
TaqI TCGA 4 cut(s) 77, 211, 261, 288
TasI AATT 4 cut(s) 98, 125, 192, 249
TatI WGTACW 2 cut(s) 143, 222
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TseI GCWGC 1 cut(s) 14
TspDTI ATGAA 6 cut(s) 17, 21, 91, 111, 118, 306
XapI RAATTY 3 cut(s) 98, 125, 249
XceI RCATGY 1 cut(s) 283
XhoI CTCGAG 1 cut(s) 210
ZrmI AGTACT 2 cut(s) 145, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.