Rorug05G0262700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001240
Physical Location & Seq
Reverse (-)
29744001 .. 29745336
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0262700.1

Sequence Viewer

Length: 873 bp
ATGGAGATACACCTCTACATGTGGCTGCAAGTGTCAGAGGTAGACCTGGGGCTTGAGACTCTGAGAATTAGAAATAGGGGGAACAACACAGTATTACACGAAGCTTCAAGGAATGGTCACTATAAAGTGGTCGAGTTCTTAATCAAAGTTGATCCTAACTTGGCTTCTGTTGAAAATGATGAGGGTGAGTCTCCATTGTATCTGGCCGCAAGAGGGGGAATGTTAGAGATTGTGAATCAAATTATAAGGACAACTCCGTCATCAGCTCATGGTGGTTCAGATGGCCAAACAGCTTTGCATGCTGCTGTGGTTGAGAGGCATATTGGTATCATGGAAGCTCTGGTAGCATTCAAGCAAGAACTAGTCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGTAGCATCCCTAGGTGACCACAGAACAGTTCAGCGATTACTAGAACTTGATACTTCTACTGCATATGTCTTGGATAAAGATGGTCGTTCACCAATTCATGTTGCAGCTAGGAATGGTCATACCGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCGGGAGAACTTGTTGACCCTTATGGACAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGAAGGGTTGATAAATCAGTCTGATATTGATGGAAACACGCCTTTGCATCTAGCAGCGATTGCAAGAAAAACTTGGATTCTTTGGTACTTGAGGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAATATGGCCAAACCGCATTTGACAATGATAGGTCAATGAAGAAATCTAGTAATATTACATCTCCCATGGTGAGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

32.26

Weight (kDa)

6.05

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 25 - 82 1.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 66 - 151 2.8e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 137 - 218 4.3e-14 Ankyrin repeats (3 copies)
Ank_5 PF13857 191 - 240 9.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 194 - 253 7.8e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AasI GACNNNNNNGTC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 42
AciI CCGC 2 cut(s) 207, 807
AclWI GGATC 2 cut(s) 146, 777
AcoI YGGCCR 3 cut(s) 204, 283, 799
AfaI GTAC 2 cut(s) 752, 868
AfiI CCNNNNNNNGG 3 cut(s) 213, 565, 566
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 527
AgsI TTSAA 3 cut(s) 108, 173, 352
AhlI ACTAGT 1 cut(s) 361
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 7 cut(s) 104, 266, 293, 338, 374, 512, 629
AluI AGCT 7 cut(s) 104, 266, 293, 338, 374, 512, 629
Alw26I GTCTC 2 cut(s) 50, 195
AlwI GGATC 2 cut(s) 146, 777
AoxI GGCC 4 cut(s) 204, 283, 385, 799
ApeKI GCWGC 4 cut(s) 25, 302, 509, 719
AsiGI ACCGGT 1 cut(s) 527
AspA2I CCTAGG 1 cut(s) 415
AsuHPI GGTGA 3 cut(s) 197, 431, 486
AvrII CCTAGG 1 cut(s) 415
BalI TGGCCA 2 cut(s) 285, 801
BbvI GCAGC 4 cut(s) 12, 289, 521, 731
BccI CCATC 4 cut(s) 275, 479, 689, 758
BciT130I CCWGG 1 cut(s) 47
BcoDI GTCTC 2 cut(s) 50, 195
BcuI ACTAGT 1 cut(s) 361
BfaI CTAG 7 cut(s) 362, 416, 446, 513, 665, 716, 840
BisI GCNGC 5 cut(s) 26, 207, 303, 510, 720
BlnI CCTAGG 1 cut(s) 415
BlsI GCNGC 5 cut(s) 27, 208, 304, 511, 721
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 1 cut(s) 47
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 2 cut(s) 419, 721
BpuEI CTTGAG 2 cut(s) 74, 775
BsaJI CCNNGG 3 cut(s) 46, 415, 858
BsaWI WCCGGW 1 cut(s) 527
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 565, 566
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 553
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 3 cut(s) 46, 415, 858
BseGI GGATG 2 cut(s) 410, 769
BseLI CCNNNNNNNGG 3 cut(s) 213, 565, 566
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 1 cut(s) 384
BseXI GCAGC 4 cut(s) 12, 289, 521, 731
BshFI GGCC 4 cut(s) 206, 285, 387, 801
BshTI ACCGGT 1 cut(s) 527
BsiSI CCGG 2 cut(s) 388, 528
BslI CCNNNNNNNGG 3 cut(s) 213, 565, 566
BsmAI GTCTC 2 cut(s) 50, 195
BsmI GAATGC 2 cut(s) 347, 601
BsnI GGCC 4 cut(s) 206, 285, 387, 801
Bsp143I GATC 3 cut(s) 151, 376, 782
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 2 cut(s) 207, 807
BspANI GGCC 4 cut(s) 206, 285, 387, 801
BspCNI CTCAG 1 cut(s) 54
BspPI GGATC 2 cut(s) 146, 777
BspQI GCTCTTC 1 cut(s) 606
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 527
BsrI ACTGG 1 cut(s) 618
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 3 cut(s) 46, 415, 858
BssMI GATC 3 cut(s) 151, 376, 782
BssT1I CCWWGG 2 cut(s) 415, 858
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 2 cut(s) 91, 433
Bst6I CTCTTC 2 cut(s) 606, 763
BstAPI GCANNNNNTGC 1 cut(s) 725
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 1 cut(s) 62
BstDSI CCRYGG 1 cut(s) 858
BstEII GGTNACC 1 cut(s) 419
BstF5I GGATG 2 cut(s) 410, 769
BstKTI GATC 3 cut(s) 154, 379, 785
BstMAI GTCTC 2 cut(s) 50, 195
BstMBI GATC 3 cut(s) 151, 376, 782
BstMWI GCNNNNNNNGC 3 cut(s) 299, 344, 725
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 2 cut(s) 22, 302
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 45
BstV1I GCAGC 4 cut(s) 12, 289, 521, 731
BstX2I RGATCY 1 cut(s) 782
BstYI RGATCY 1 cut(s) 782
BsuRI GGCC 4 cut(s) 206, 285, 387, 801
BtgI CCRYGG 1 cut(s) 858
BtsCI GGATG 2 cut(s) 410, 769
BtsIMutI CAGTG 1 cut(s) 625
Cac8I GCNNGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 527
Csp6I GTAC 2 cut(s) 751, 867
CspAI ACCGGT 1 cut(s) 527
CviAII CATG 6 cut(s) 19, 269, 299, 331, 503, 859
CviQI GTAC 2 cut(s) 751, 867
DdeI CTNAG 1 cut(s) 62
DpnI GATC 3 cut(s) 153, 378, 784
DpnII GATC 3 cut(s) 151, 376, 782
DrdI GACNNNNNNGTC 1 cut(s) 256
DseDI GACNNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 3 cut(s) 204, 283, 799
Eam1104I CTCTTC 2 cut(s) 606, 763
EarI CTCTTC 2 cut(s) 606, 763
Eco130I CCWWGG 2 cut(s) 415, 858
Eco91I GGTNACC 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 2 cut(s) 415, 858
ErhI CCWWGG 2 cut(s) 415, 858
FaeI CATG 6 cut(s) 22, 272, 302, 334, 506, 862
FalI AAGNNNNNCTT 2 cut(s) 721, 753
FatI CATG 6 cut(s) 18, 268, 298, 330, 502, 858
FauNDI CATATG 1 cut(s) 469
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 5 cut(s) 26, 207, 303, 510, 720
FokI GGATG 2 cut(s) 397, 776
Fsp4HI GCNGC 5 cut(s) 26, 207, 303, 510, 720
FspBI CTAG 7 cut(s) 362, 416, 446, 513, 665, 716, 840
GluI GCNGC 5 cut(s) 26, 207, 303, 510, 720
HaeIII GGCC 4 cut(s) 206, 285, 387, 801
HapII CCGG 2 cut(s) 388, 528
Hin1II CATG 6 cut(s) 22, 272, 302, 334, 506, 862
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 6 cut(s) 58, 188, 235, 391, 563, 742
HpaII CCGG 2 cut(s) 388, 528
HphI GGTGA 3 cut(s) 197, 431, 486
Hpy166II GTNNAC 4 cut(s) 43, 494, 580, 623
Hpy188I TCNGA 4 cut(s) 37, 63, 280, 688
Hpy188III TCNNGA 1 cut(s) 567
Hpy8I GTNNAC 4 cut(s) 43, 494, 580, 623
HpyAV CCTTC 1 cut(s) 662
HpyCH4III ACNGT 2 cut(s) 91, 433
HpyCH4V TGCA 6 cut(s) 28, 298, 467, 509, 712, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 344, 725
HpyF3I CTNAG 1 cut(s) 62
Hsp92II CATG 6 cut(s) 22, 272, 302, 334, 506, 862
Kzo9I GATC 3 cut(s) 151, 376, 782
LguI GCTCTTC 1 cut(s) 606
LpnPI CCDG 9 cut(s) 32, 59, 188, 326, 401, 541, 573, 631, 672
Lsp1109I GCAGC 4 cut(s) 12, 289, 521, 731
LweI GCATC 2 cut(s) 419, 721
MaeI CTAG 7 cut(s) 362, 416, 446, 513, 665, 716, 840
MaeIII GTNAC 3 cut(s) 116, 419, 637
MalI GATC 3 cut(s) 153, 378, 784
MboI GATC 3 cut(s) 151, 376, 782
MboII GAAGA 3 cut(s) 593, 780, 844
MflI RGATCY 1 cut(s) 782
MlsI TGGCCA 2 cut(s) 285, 801
MluCI AATT 3 cut(s) 66, 240, 498
MluNI TGGCCA 2 cut(s) 285, 801
MlyI GAGTC 3 cut(s) 52, 197, 385
MmeI TCCRAC 1 cut(s) 574
MnlI CCTC 7 cut(s) 23, 31, 175, 206, 309, 405, 750
Mox20I TGGCCA 2 cut(s) 285, 801
MscI TGGCCA 2 cut(s) 285, 801
MseI TTAA 2 cut(s) 140, 871
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 2 cut(s) 285, 801
MspI CCGG 2 cut(s) 388, 528
MspR9I CCNGG 1 cut(s) 47
Mva1269I GAATGC 2 cut(s) 347, 601
MvaI CCWGG 1 cut(s) 47
MwoI GCNNNNNNNGC 3 cut(s) 299, 344, 725
NcoI CCATGG 1 cut(s) 858
NdeI CATATG 1 cut(s) 469
NdeII GATC 3 cut(s) 151, 376, 782
NlaIII CATG 6 cut(s) 22, 272, 302, 334, 506, 862
NmuCI GTSAC 3 cut(s) 116, 419, 637
NspI RCATGY 2 cut(s) 22, 302
PaeI GCATGC 1 cut(s) 302
PciI ACATGT 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 606
PctI GAATGC 2 cut(s) 347, 601
PfeI GAWTC 3 cut(s) 235, 563, 742
PinAI ACCGGT 1 cut(s) 527
PkrI GCNGC 5 cut(s) 27, 208, 304, 511, 721
PleI GAGTC 3 cut(s) 52, 196, 385
PpsI GAGTC 3 cut(s) 52, 196, 385
PscI ACATGT 1 cut(s) 18
PsiI TTATAA 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 45
PspEI GGTNACC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 45
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 2 cut(s) 752, 868
RsaNI GTAC 2 cut(s) 751, 867
RseI CAYNNNNRTG 1 cut(s) 528
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 2 cut(s) 140, 871
SatI GCNGC 5 cut(s) 26, 207, 303, 510, 720
Sau3AI GATC 3 cut(s) 151, 376, 782
ScaI AGTACT 1 cut(s) 868
SchI GAGTC 3 cut(s) 52, 197, 385
ScrFI CCNGG 1 cut(s) 47
SfaNI GCATC 2 cut(s) 419, 721
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 2 cut(s) 53, 754
SmoI CTYRAG 2 cut(s) 53, 754
SpeI ACTAGT 1 cut(s) 361
SphI GCATGC 1 cut(s) 302
Sse9I AATT 3 cut(s) 66, 240, 498
SsiI CCGC 2 cut(s) 207, 807
SspI AATATT 1 cut(s) 847
SspMI CTAG 7 cut(s) 362, 416, 446, 513, 665, 716, 840
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 2 cut(s) 415, 858
TaaI ACNGT 2 cut(s) 91, 433
TaqI TCGA 2 cut(s) 132, 379
TasI AATT 3 cut(s) 66, 240, 498
TatI WGTACW 1 cut(s) 866
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 3 cut(s) 235, 563, 742
Tru1I TTAA 2 cut(s) 140, 871
Tru9I TTAA 2 cut(s) 140, 871
TscAI CASTG 1 cut(s) 625
TseFI GTSAC 3 cut(s) 116, 419, 637
TseI GCWGC 4 cut(s) 25, 302, 509, 719
Tsp45I GTSAC 3 cut(s) 116, 419, 637
TspDTI ATGAA 3 cut(s) 491, 593, 845
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 1 cut(s) 625
XceI RCATGY 2 cut(s) 22, 302
XmaJI CCTAGG 1 cut(s) 415
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 7 cut(s) 362, 416, 446, 513, 665, 716, 840
ZrmI AGTACT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.