pycom01g14100

Amino-transferase class IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004670
Physical Location & Seq
Reverse (-)
14366015 .. 14368956
2942 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14100.2

Sequence Viewer

Length: 1047 bp
ATGGCGCGTTTCTTGTTCACCAACGGCGTTGTTTCGCCACCCGCGACTACTCCTCCGGTCGCCGCCTTCCTCGAAACCCACCCAGGCGCGTACACGACGTCCCGAACTCACAACGACGCGTCGTTCGTGCTGTTCTGGGAAAAGCACCTGAGGAGACTGGCGGACTCTGTGACAATCCTCTACAATTCGAATCCTCAACTTCTCTTCGGACCCCACTGCAAAACGACGCCGCCTTCGCTGCCGTCTCTGTCGTCCGACTCCATCTCGCTCCGATCGACGATTCGCGAACTCGTGAACCGTTCGATGAAGCAAGTGCTGCCGATTGCTATGGAGGAGAGGAGGGAGGGGGAGGAGCTGTCGATTACGGCGTTGGTCAGTGGCAATTTGGAGAAATTGAGAGTTGGGGATGAGGGTTTTGATGTGAGTATCTACATTGGGCGTTATGCGCCTGCTGTGTTTGGTATCAAAGAAAACGGTGCGTTTTTGGCTGTGGTGGGTCGCGGGAGAGATTTAGCTGCTGCAAAGTACTCGGATTGGGTAAGGATGAGGAAGTCTTTGGAGAGGTTGAGGCCGCCGAAAGGGACGGAGCTCTTGTTGTCCAATGATGGTGATCGGATTCTTGAGGGCACCGTGTCTAACTTGTTCGTTGTCCGCCGGAAGGATGTCAGTGAAGCTAAAGCCAAAAGTATGCACTGTTTTGAAGTACAGACAGCTCCTCTTAGTGGTGTCCTTCCAGGAATTATTCGGCAAGTGGTCATTGAAGTCTGCTTGAGCAAAGGAATTCCCTTGAGAGAAGTTGCACCATTGTGGTCAGAGAGAGAATATTGGGCAGAGGCATTCATTACAAGCAGCTTGAGACTTCTGCAGCACGCGAAGACAATTAGTTTTCCAAGTTCATGGGAATCACTGGATTCTAATTCTTTGGAGAACATCACATGGATAGACAAGCATTTTGAGGACGGCCCTGGGATGATCACGACAATAATCCAGAAAGAGGTCATGGAAAGGGCAGTTTTAGAAGGGTACTCTGTCAGTTACTTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

349

Amino Acids

38.81

Weight (kDa)

7.07

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 626
AccII CGCG 7 cut(s) 7, 44, 89, 119, 285, 501, 872
AciI CCGC 7 cut(s) 42, 63, 161, 230, 501, 572, 652
AcsI RAATTY 1 cut(s) 780
AcyI GRCGYC 2 cut(s) 98, 227
AfaI GTAC 4 cut(s) 92, 527, 705, 1025
AfiI CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 2 cut(s) 701, 761
AjnI CCWGG 3 cut(s) 82, 733, 964
AleI CACNNNNGTG 1 cut(s) 805
AluBI AGCT 6 cut(s) 355, 515, 589, 674, 713, 852
AluI AGCT 6 cut(s) 355, 515, 589, 674, 713, 852
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 3 cut(s) 148, 249, 850
AoxI GGCC 2 cut(s) 569, 961
ApeKI GCWGC 6 cut(s) 238, 316, 515, 518, 849, 865
ApoI RAATTY 1 cut(s) 780
ArsI GACNNNNNNTTYG 6 cut(s) 107, 139, 217, 249, 935, 967
AspLEI GCGC 3 cut(s) 7, 89, 448
AspS9I GGNCC 2 cut(s) 209, 962
AsuHPI GGTGA 2 cut(s) 10, 620
AsuII TTCGAA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 1 cut(s) 591
BauI CACGAG 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 881
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
BccI CCATC 2 cut(s) 269, 599
BceAI ACGGC 4 cut(s) 40, 226, 381, 976
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 3 cut(s) 84, 735, 966
BclI TGATCA 1 cut(s) 972
BcoDI GTCTC 3 cut(s) 148, 249, 850
BfmI CTRYAG 1 cut(s) 863
BisI GCNGC 9 cut(s) 63, 230, 239, 317, 516, 519, 572, 850, 866
BlsI GCNGC 9 cut(s) 64, 231, 240, 318, 517, 520, 573, 851, 867
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 3 cut(s) 84, 735, 966
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 209, 962
BmiI GGNNCC 2 cut(s) 211, 628
BmrFI CCNGG 3 cut(s) 84, 735, 966
BpiI GAAGAC 1 cut(s) 881
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 4 cut(s) 641, 790, 808, 874
BsaBI GATNNNNATC 1 cut(s) 609
BsaHI GRCGYC 2 cut(s) 98, 227
BsaJI CCNNGG 3 cut(s) 82, 964, 965
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 6 cut(s) 37, 67, 341, 371, 578, 608
Bsc4I CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
Bse1I ACTGG 2 cut(s) 162, 912
Bse21I CCTNAGG 1 cut(s) 149
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 3 cut(s) 84, 735, 966
BseDI CCNNGG 3 cut(s) 82, 964, 965
BseGI GGATG 4 cut(s) 412, 549, 667, 975
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 162, 912
BseRI GAGGAG 6 cut(s) 42, 166, 347, 352, 365, 705
BseSI GKGCMC 1 cut(s) 629
BseXI GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
Bsh1236I CGCG 7 cut(s) 7, 44, 89, 119, 285, 501, 872
Bsh1285I CGRYCG 2 cut(s) 60, 275
BshFI GGCC 2 cut(s) 571, 963
BshNI GGYRCC 1 cut(s) 626
BsiEI CGRYCG 2 cut(s) 60, 275
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 2 cut(s) 56, 655
BslFI GGGAC 2 cut(s) 85, 595
BslI CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
BsmAI GTCTC 3 cut(s) 148, 249, 850
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 2 cut(s) 85, 595
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 2 cut(s) 571, 963
Bsp119I TTCGAA 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 591, 629
Bsp143I GATC 3 cut(s) 272, 610, 972
Bsp68I TCGCGA 1 cut(s) 285
BspACI CCGC 7 cut(s) 42, 63, 161, 230, 501, 572, 652
BspANI GGCC 2 cut(s) 571, 963
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 7 cut(s) 7, 44, 89, 119, 285, 501, 872
BspLI GGNNCC 2 cut(s) 211, 628
BspMAI CTGCAG 1 cut(s) 867
BspT104I TTCGAA 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 626
BsrI ACTGG 2 cut(s) 162, 912
BssECI CCNNGG 3 cut(s) 82, 964, 965
BssMI GATC 3 cut(s) 272, 610, 972
BssNI GRCGYC 2 cut(s) 98, 227
BssSI CACGAG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 290
Bst2UI CCWGG 3 cut(s) 84, 735, 966
Bst4CI ACNGT 4 cut(s) 299, 476, 631, 695
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 2 cut(s) 98, 227
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 450, 870
BstDEI CTNAG 2 cut(s) 149, 719
BstF5I GGATG 4 cut(s) 412, 549, 667, 975
BstFNI CGCG 7 cut(s) 7, 44, 89, 119, 285, 501, 872
BstHHI GCGC 3 cut(s) 7, 89, 448
BstKTI GATC 3 cut(s) 275, 613, 975
BstMAI GTCTC 3 cut(s) 148, 249, 850
BstMBI GATC 3 cut(s) 272, 610, 972
BstMCI CGRYCG 2 cut(s) 60, 275
BstMWI GCNNNNNNNGC 5 cut(s) 235, 238, 316, 445, 485
BstNI CCWGG 3 cut(s) 84, 735, 966
BstSCI CCNGG 3 cut(s) 82, 733, 964
BstSFI CTRYAG 1 cut(s) 863
BstSLI GKGCMC 1 cut(s) 629
BstUI CGCG 7 cut(s) 7, 44, 89, 119, 285, 501, 872
BstV1I GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
BstV2I GAAGAC 1 cut(s) 881
BstXI CCANNNNNNTGG 1 cut(s) 897
Bsu36I CCTNAGG 1 cut(s) 149
BsuRI GGCC 2 cut(s) 571, 963
BtsCI GGATG 4 cut(s) 412, 549, 667, 975
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 5 cut(s) 214, 382, 673, 691, 905
BtuMI TCGCGA 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 450, 870
CfoI GCGC 3 cut(s) 7, 89, 448
Cfr13I GGNCC 2 cut(s) 209, 962
CseI GACGC 3 cut(s) 108, 125, 235
Csp6I GTAC 4 cut(s) 91, 526, 704, 1024
CviAII CATG 3 cut(s) 897, 936, 1000
CviQI GTAC 4 cut(s) 91, 526, 704, 1024
DdeI CTNAG 2 cut(s) 149, 719
DpnI GATC 3 cut(s) 274, 612, 974
DpnII GATC 3 cut(s) 272, 610, 972
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
EciI GGCGGA 2 cut(s) 176, 641
Ecl136II GAGCTC 1 cut(s) 589
Eco24I GRGCYC 1 cut(s) 591
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 589
Eco81I CCTNAGG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 589
EcoRI GAATTC 1 cut(s) 780
EcoRII CCWGG 3 cut(s) 82, 733, 964
EcoT38I GRGCYC 1 cut(s) 591
Esp3I CGTCTC 1 cut(s) 249
FaeI CATG 3 cut(s) 900, 939, 1003
FaiI YATR 7 cut(s) 329, 444, 689, 898, 937, 1001, 1045
FaqI GGGAC 2 cut(s) 85, 595
FatI CATG 3 cut(s) 896, 935, 999
FauI CCCGC 2 cut(s) 49, 494
FbaI TGATCA 1 cut(s) 972
Fnu4HI GCNGC 9 cut(s) 63, 230, 239, 317, 516, 519, 572, 850, 866
FokI GGATG 4 cut(s) 419, 556, 674, 982
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 9 cut(s) 63, 230, 239, 317, 516, 519, 572, 850, 866
GlaI GCGC 3 cut(s) 6, 88, 447
GluI GCNGC 9 cut(s) 63, 230, 239, 317, 516, 519, 572, 850, 866
HaeIII GGCC 2 cut(s) 571, 963
HapII CCGG 2 cut(s) 56, 655
HgaI GACGC 3 cut(s) 108, 125, 235
HhaI GCGC 3 cut(s) 7, 89, 448
Hin1I GRCGYC 2 cut(s) 98, 227
Hin1II CATG 3 cut(s) 900, 939, 1003
Hin6I GCGC 3 cut(s) 5, 87, 446
HinP1I GCGC 3 cut(s) 5, 87, 446
HinfI GANTC 7 cut(s) 164, 190, 257, 280, 616, 902, 911
HpaII CCGG 2 cut(s) 56, 655
HphI GGTGA 2 cut(s) 10, 620
Hpy166II GTNNAC 3 cut(s) 18, 93, 295
Hpy188I TCNGA 6 cut(s) 209, 256, 272, 532, 615, 814
Hpy188III TCNNGA 6 cut(s) 102, 284, 292, 620, 976, 988
Hpy8I GTNNAC 3 cut(s) 18, 93, 295
Hpy99I CGWCG 5 cut(s) 100, 119, 124, 229, 280
HpyAV CCTTC 5 cut(s) 76, 243, 652, 740, 1013
HpyCH4III ACNGT 4 cut(s) 299, 476, 631, 695
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 5 cut(s) 219, 521, 691, 800, 865
HpyF10VI GCNNNNNNNGC 5 cut(s) 235, 238, 316, 445, 485
HpyF3I CTNAG 2 cut(s) 149, 719
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 2 cut(s) 98, 227
Hsp92II CATG 3 cut(s) 900, 939, 1003
HspAI GCGC 3 cut(s) 5, 87, 446
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 3 cut(s) 272, 610, 972
LmnI GCTCC 4 cut(s) 273, 352, 586, 718
Lsp1109I GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 169, 1034
MalI GATC 3 cut(s) 274, 612, 974
MboI GATC 3 cut(s) 272, 610, 972
MboII GAAGA 2 cut(s) 196, 886
MhlI GDGCHC 2 cut(s) 591, 629
MluCI AATT 7 cut(s) 184, 382, 392, 738, 780, 879, 916
MluI ACGCGT 1 cut(s) 117
MlyI GAGTC 2 cut(s) 158, 251
MmeI TCCRAC 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 805
MspI CCGG 2 cut(s) 56, 655
MspR9I CCNGG 3 cut(s) 84, 735, 966
Mva1269I GAATGC 1 cut(s) 836
MvaI CCWGG 3 cut(s) 84, 735, 966
MvnI CGCG 7 cut(s) 7, 44, 89, 119, 285, 501, 872
MwoI GCNNNNNNNGC 5 cut(s) 235, 238, 316, 445, 485
NdeII GATC 3 cut(s) 272, 610, 972
NlaIII CATG 3 cut(s) 900, 939, 1003
NlaIV GGNNCC 2 cut(s) 211, 628
NmuCI GTSAC 1 cut(s) 169
NruI TCGCGA 1 cut(s) 285
NspV TTCGAA 1 cut(s) 188
OliI CACNNNNGTG 1 cut(s) 805
PasI CCCWGGG 1 cut(s) 965
PcsI WCGNNNNNNNCGW 4 cut(s) 41, 123, 272, 365
PctI GAATGC 1 cut(s) 836
PfeI GAWTC 5 cut(s) 190, 280, 616, 902, 911
PfoI TCCNGGA 1 cut(s) 733
PkrI GCNGC 9 cut(s) 64, 231, 240, 318, 517, 520, 573, 851, 867
Ple19I CGATCG 1 cut(s) 275
PleI GAGTC 2 cut(s) 158, 251
PpsI GAGTC 2 cut(s) 158, 251
Psp124BI GAGCTC 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 82, 733, 964
PspGI CCWGG 3 cut(s) 82, 733, 964
PspN4I GGNNCC 2 cut(s) 211, 628
PspPI GGNCC 2 cut(s) 209, 962
PstI CTGCAG 1 cut(s) 867
PvuI CGATCG 1 cut(s) 275
RruI TCGCGA 1 cut(s) 285
RsaI GTAC 4 cut(s) 92, 527, 705, 1025
RsaNI GTAC 4 cut(s) 91, 526, 704, 1024
RseI CAYNNNNRTG 1 cut(s) 805
SacI GAGCTC 1 cut(s) 591
SatI GCNGC 9 cut(s) 63, 230, 239, 317, 516, 519, 572, 850, 866
Sau3AI GATC 3 cut(s) 272, 610, 972
Sau96I GGNCC 2 cut(s) 209, 962
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 158, 251
ScrFI CCNGG 3 cut(s) 84, 735, 966
SduI GDGCHC 2 cut(s) 591, 629
SfcI CTRYAG 1 cut(s) 863
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 805
SmlI CTYRAG 4 cut(s) 620, 769, 787, 853
SmoI CTYRAG 4 cut(s) 620, 769, 787, 853
Sse9I AATT 7 cut(s) 184, 382, 392, 738, 780, 879, 916
SsiI CCGC 7 cut(s) 42, 63, 161, 230, 501, 572, 652
SspI AATATT 1 cut(s) 824
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 3 cut(s) 82, 733, 964
TaaI ACNGT 4 cut(s) 299, 476, 631, 695
TaiI ACGT 1 cut(s) 101
TaqI TCGA 5 cut(s) 72, 188, 275, 302, 359
TasI AATT 7 cut(s) 184, 382, 392, 738, 780, 879, 916
TatI WGTACW 2 cut(s) 525, 703
TauI GCSGC 3 cut(s) 65, 232, 574
TfiI GAWTC 5 cut(s) 190, 280, 616, 902, 911
TscAI CASTG 5 cut(s) 221, 382, 673, 698, 912
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 6 cut(s) 238, 316, 515, 518, 849, 865
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 3 cut(s) 320, 829, 885
TspGWI ACGGA 1 cut(s) 599
TspRI CASTG 5 cut(s) 221, 382, 673, 698, 912
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 780
ZraI GACGTC 1 cut(s) 99
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.