Rh1BG285200

Amino-transferase class IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004670
Physical Location & Seq
Reverse (-)
42132752 .. 42136273
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG285200.1

Sequence Viewer

Length: 930 bp
ATGACGACTACTCCTCTCAGGTTCTTGCTCACCAACGGCGTCGTCTCCAACCCTGCCAACACTCCTCCCGTCACCACCTTCCTCGAGACCCACCCAGGGGCTTACACGACGACGCGCACTCACAACAACGCCTCATTCGTGTTGTTTTGGGATAGGCACTTGAAAAGGCTCGCGGAGTCCGTGAGAATCCTCTACAATGCCGATCCTCAGCTTCTTTTCGGACCCAACAAGACTTCGCTTCCATTACCGTCGTCTCTTGCAGCCAATTCTCTTTTGCTCCAGTCAAAGGTCAATGATTCGATGAAAAAAGTGCTGCCAATTGCTTTGGAGGGGAGGAGTGACGGGGAGGAACTGTCGATTACTACTTTGGTCAGCGGCAATTTGGGGAAATTGGGTGAGAGTGGGGACTTGGAGCCTCTTGATGTGAGCATTTACGTTGGGATTTATGTGCCTTCTGTGTTTGGTGTCAGAGGAAACGGTGCCCAGTTGGCTGTGGTGGGTCGTGGGAGAGATGTTGCTGCTGCTAAGTACTCGGATTGGGTGAGGATTAGGAAGTCTTTGGAGAGGTTGAGGCCGCCCTCAGGGACCGAGCTCTTGTTGTCCAATGATGGGGATCGGATTCTTGAAGGCAGCCTGTCAAACTTCTTTGTGGTCCGCCGAAAGGATGACAATGAAGCTAAAGAGCAAACTATCCACTGTTTTGAAGTACAGACAGCTCCTATTAGCGAGGGTGTCCTACCAGGAATTATACGCCAACTAGTCATTGAAGTATGCTTGAGCAAAGGAATCCCTTTTCGAGAAGTTGCACCACTGTGGTCAGAGCATGAATTTTGGGAAGAGGCGTTCATTACAAGTAAATCTCTACTTATCTCTTTCTTCAGTTCCATGCAATTACGTAGTTATCGACACGTATTTTCGCCTCGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

309

Amino Acids

34.17

Weight (kDa)

7.8

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 197 - 285 2.1e-08 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccII CGCG 2 cut(s) 115, 173
AciI CCGC 4 cut(s) 173, 375, 575, 655
AclWI GGATC 2 cut(s) 197, 621
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 1 cut(s) 862
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 530, 708
AfiI CCNNNNNNNGG 6 cut(s) 96, 97, 286, 581, 609, 661
AflIII ACRYGT 1 cut(s) 907
AgsI TTSAA 4 cut(s) 163, 626, 704, 767
AhlI ACTAGT 1 cut(s) 757
AjnI CCWGG 2 cut(s) 94, 739
AleI CACNNNNGTG 1 cut(s) 811
AloI GAACNNNNNNTCC 2 cut(s) 827, 859
AluBI AGCT 4 cut(s) 211, 592, 677, 716
AluI AGCT 4 cut(s) 211, 592, 677, 716
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 3 cut(s) 49, 80, 258
AlwI GGATC 2 cut(s) 197, 621
Ama87I CYCGRG 2 cut(s) 83, 921
AoxI GGCC 1 cut(s) 572
ApeKI GCWGC 5 cut(s) 260, 313, 518, 521, 630
ApoI RAATTY 1 cut(s) 827
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 3 cut(s) 221, 585, 652
AsuHPI GGTGA 4 cut(s) 22, 64, 407, 553
AvaI CYCGRG 2 cut(s) 83, 921
AvaII GGWCC 3 cut(s) 221, 585, 652
AxyI CCTNAGG 1 cut(s) 580
BaeGI GKGCMC 1 cut(s) 484
BanI GGYRCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 594
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 5 cut(s) 272, 300, 505, 508, 642
BccI CCATC 1 cut(s) 602
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 2 cut(s) 96, 741
BcoDI GTCTC 3 cut(s) 49, 80, 258
BcuI ACTAGT 1 cut(s) 757
BfaI CTAG 1 cut(s) 758
BglI GCCNNNNNGGC 1 cut(s) 488
BisI GCNGC 7 cut(s) 261, 314, 376, 519, 522, 575, 631
BlsI GCNGC 7 cut(s) 262, 315, 377, 520, 523, 576, 632
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 96, 741
Bme18I GGWCC 3 cut(s) 221, 585, 652
BmeT110I CYCGRG 2 cut(s) 83, 921
BmgT120I GGNCC 3 cut(s) 221, 585, 652
BmiI GGNNCC 4 cut(s) 223, 414, 481, 586
BmrFI CCNGG 2 cut(s) 96, 741
BmrI ACTGGG 1 cut(s) 478
BmuI ACTGGG 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 263
Bpu10I CCTNAGC 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 796
BsaAI YACGTR 2 cut(s) 896, 910
BsaBI GATNNNNATC 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 94, 95, 920
BsaXI ACNNNNNCTCC 3 cut(s) 25, 338, 368
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 97, 286, 581, 609, 661
Bse1I ACTGG 2 cut(s) 280, 484
Bse21I CCTNAGG 1 cut(s) 580
Bse8I GATNNNNATC 1 cut(s) 612
BseBI CCWGG 2 cut(s) 96, 741
BseDI CCNNGG 3 cut(s) 94, 95, 920
BseGI GGATG 1 cut(s) 670
BseJI GATNNNNATC 1 cut(s) 612
BseLI CCNNNNNNNGG 6 cut(s) 96, 97, 286, 581, 609, 661
BseMII CTCAG 3 cut(s) 31, 221, 594
BseNI ACTGG 2 cut(s) 280, 484
BseRI GAGGAG 2 cut(s) 54, 349
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 5 cut(s) 272, 300, 505, 508, 642
Bsh1236I CGCG 2 cut(s) 115, 173
BshFI GGCC 1 cut(s) 574
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 2 cut(s) 83, 921
BslFI GGGAC 2 cut(s) 419, 598
BslI CCNNNNNNNGG 6 cut(s) 96, 97, 286, 581, 609, 661
BsmAI GTCTC 3 cut(s) 49, 80, 258
BsmBI CGTCTC 2 cut(s) 49, 258
BsmFI GGGAC 2 cut(s) 419, 598
BsnI GGCC 1 cut(s) 574
Bso31I GGTCTC 1 cut(s) 80
BsoBI CYCGRG 2 cut(s) 83, 921
Bsp1286I GDGCHC 2 cut(s) 484, 594
Bsp143I GATC 2 cut(s) 202, 613
BspACI CCGC 4 cut(s) 173, 375, 575, 655
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 3 cut(s) 30, 220, 593
BspFNI CGCG 2 cut(s) 115, 173
BspLI GGNNCC 4 cut(s) 223, 414, 481, 586
BspPI GGATC 2 cut(s) 197, 621
BspT107I GGYRCC 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 80
BsrI ACTGG 2 cut(s) 280, 484
BssECI CCNNGG 3 cut(s) 94, 95, 920
BssMI GATC 2 cut(s) 202, 613
BssNI GRCGYC 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 96, 741
Bst4CI ACNGT 5 cut(s) 249, 354, 479, 698, 813
Bst6I CTCTTC 1 cut(s) 831
BstACI GRCGYC 1 cut(s) 39
BstBAI YACGTR 2 cut(s) 896, 910
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 4 cut(s) 17, 207, 525, 580
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 2 cut(s) 115, 173
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 2 cut(s) 205, 616
BstMAI GTCTC 3 cut(s) 49, 80, 258
BstMBI GATC 2 cut(s) 202, 613
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 2 cut(s) 96, 741
BstSCI CCNGG 2 cut(s) 94, 739
BstSLI GKGCMC 1 cut(s) 484
BstSNI TACGTA 1 cut(s) 896
BstUI CGCG 2 cut(s) 115, 173
BstV1I GCAGC 5 cut(s) 272, 300, 505, 508, 642
Bsu36I CCTNAGG 1 cut(s) 580
BsuRI GGCC 1 cut(s) 574
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 2 cut(s) 694, 809
Cac8I GCNNGC 1 cut(s) 171
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 3 cut(s) 221, 585, 652
CseI GACGC 2 cut(s) 28, 121
Csp6I GTAC 2 cut(s) 529, 707
CviAII CATG 2 cut(s) 824, 886
CviQI GTAC 2 cut(s) 529, 707
DdeI CTNAG 4 cut(s) 17, 207, 525, 580
DpnI GATC 2 cut(s) 204, 615
DpnII GATC 2 cut(s) 202, 613
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
EciI GGCGGA 1 cut(s) 644
Ecl136II GAGCTC 1 cut(s) 592
Eco105I TACGTA 1 cut(s) 896
Eco24I GRGCYC 1 cut(s) 594
Eco31I GGTCTC 1 cut(s) 80
Eco47I GGWCC 3 cut(s) 221, 585, 652
Eco53kI GAGCTC 1 cut(s) 592
Eco57I CTGAAG 1 cut(s) 862
Eco81I CCTNAGG 1 cut(s) 580
Eco88I CYCGRG 2 cut(s) 83, 921
EcoICRI GAGCTC 1 cut(s) 592
EcoRII CCWGG 2 cut(s) 94, 739
EcoT38I GRGCYC 1 cut(s) 594
Esp3I CGTCTC 2 cut(s) 49, 258
FaeI CATG 2 cut(s) 827, 889
FaiI YATR 5 cut(s) 447, 749, 772, 825, 887
FaqI GGGAC 2 cut(s) 419, 598
FatI CATG 2 cut(s) 823, 885
Fnu4HI GCNGC 7 cut(s) 261, 314, 376, 519, 522, 575, 631
FokI GGATG 1 cut(s) 677
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 7 cut(s) 261, 314, 376, 519, 522, 575, 631
FspBI CTAG 1 cut(s) 758
GlaI GCGC 1 cut(s) 116
GluI GCNGC 7 cut(s) 261, 314, 376, 519, 522, 575, 631
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 1 cut(s) 574
HgaI GACGC 2 cut(s) 28, 121
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 2 cut(s) 827, 889
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 5 cut(s) 176, 186, 296, 619, 786
HphI GGTGA 4 cut(s) 22, 64, 407, 553
Hpy188I TCNGA 5 cut(s) 221, 470, 535, 618, 820
Hpy188III TCNNGA 4 cut(s) 85, 419, 623, 797
Hpy99I CGWCG 4 cut(s) 44, 112, 115, 253
HpyAV CCTTC 3 cut(s) 88, 462, 620
HpyCH4III ACNGT 5 cut(s) 249, 354, 479, 698, 813
HpyCH4IV ACGT 3 cut(s) 435, 895, 909
HpyCH4V TGCA 3 cut(s) 260, 806, 889
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 4 cut(s) 17, 207, 525, 580
HpySE526I ACGT 3 cut(s) 435, 895, 909
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 2 cut(s) 827, 889
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 2 cut(s) 202, 613
LmnI GCTCC 3 cut(s) 282, 412, 721
Lsp1109I GCAGC 5 cut(s) 272, 300, 505, 508, 642
MaeI CTAG 1 cut(s) 758
MaeII ACGT 3 cut(s) 435, 895, 909
MaeIII GTNAC 2 cut(s) 70, 338
MalI GATC 2 cut(s) 204, 615
MboI GATC 2 cut(s) 202, 613
MboII GAAGA 2 cut(s) 848, 868
MfeI CAATTG 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 484, 594
MluCI AATT 7 cut(s) 265, 318, 379, 389, 744, 827, 890
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 811
MspA1I CMGCKG 1 cut(s) 375
MspR9I CCNGG 2 cut(s) 96, 741
MunI CAATTG 1 cut(s) 318
MvaI CCWGG 2 cut(s) 96, 741
MvnI CGCG 2 cut(s) 115, 173
MwoI GCNNNNNNNGC 1 cut(s) 488
NdeII GATC 2 cut(s) 202, 613
NlaIII CATG 2 cut(s) 827, 889
NlaIV GGNNCC 4 cut(s) 223, 414, 481, 586
NmuCI GTSAC 2 cut(s) 70, 338
OliI CACNNNNGTG 1 cut(s) 811
PaeR7I CTCGAG 1 cut(s) 83
PasI CCCWGGG 1 cut(s) 95
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 177, 901
PfeI GAWTC 4 cut(s) 186, 296, 619, 786
PkrI GCNGC 7 cut(s) 262, 315, 377, 520, 523, 576, 632
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Ppu21I YACGTR 2 cut(s) 896, 910
Psp124BI GAGCTC 1 cut(s) 594
Psp6I CCWGG 2 cut(s) 94, 739
PspGI CCWGG 2 cut(s) 94, 739
PspN4I GGNNCC 4 cut(s) 223, 414, 481, 586
PspPI GGNCC 3 cut(s) 221, 585, 652
RsaI GTAC 2 cut(s) 530, 708
RsaNI GTAC 2 cut(s) 529, 707
RseI CAYNNNNRTG 1 cut(s) 811
SacI GAGCTC 1 cut(s) 594
SatI GCNGC 7 cut(s) 261, 314, 376, 519, 522, 575, 631
Sau3AI GATC 2 cut(s) 202, 613
Sau96I GGNCC 3 cut(s) 221, 585, 652
ScaI AGTACT 1 cut(s) 530
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 96, 741
SduI GDGCHC 2 cut(s) 484, 594
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 3 cut(s) 221, 585, 652
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 811
SmlI CTYRAG 2 cut(s) 83, 775
SmoI CTYRAG 2 cut(s) 83, 775
SnaBI TACGTA 1 cut(s) 896
SpeI ACTAGT 1 cut(s) 757
Sse9I AATT 7 cut(s) 265, 318, 379, 389, 744, 827, 890
SsiI CCGC 4 cut(s) 173, 375, 575, 655
SspMI CTAG 1 cut(s) 758
SstI GAGCTC 1 cut(s) 594
StyD4I CCNGG 2 cut(s) 94, 739
TaaI ACNGT 5 cut(s) 249, 354, 479, 698, 813
TaiI ACGT 3 cut(s) 438, 898, 912
TaqI TCGA 5 cut(s) 84, 299, 356, 796, 904
TaqII GACCGA 1 cut(s) 602
TasI AATT 7 cut(s) 265, 318, 379, 389, 744, 827, 890
TatI WGTACW 2 cut(s) 528, 706
TauI GCSGC 2 cut(s) 378, 577
TfiI GAWTC 4 cut(s) 186, 296, 619, 786
TscAI CASTG 2 cut(s) 701, 816
TseFI GTSAC 2 cut(s) 70, 338
TseI GCWGC 5 cut(s) 260, 313, 518, 521, 630
Tsp45I GTSAC 2 cut(s) 70, 338
TspDTI ATGAA 4 cut(s) 317, 687, 835, 840
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 2 cut(s) 701, 816
VpaK11BI GGWCC 3 cut(s) 221, 585, 652
XapI RAATTY 1 cut(s) 827
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 1 cut(s) 758
ZrmI AGTACT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.