MD01G1122100.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012338
Physical Location & Seq
Forward (+)
23535950 .. 23539176
3227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1122100.v1.1.491

Sequence Viewer

Length: 1983 bp
ATGGTTTTGTCTAACAGGAAGCTGAAGCAAAAGCTCAGGGAAAAGCTAGCTCAGTCGTTAGTAGAATCGGTAGCTAAGCCTAAAACCGAGCCGACAAAAGATGGGTCGGAAAACCCAGACCCGAAATCGACCCCCCGGTCGCTGAAAGTGCTTTTGGACTCCGCAACCCAGAAACCCAGATTGTCCAAGCGGGAAAAACGCAGAAAGATCCTGTCTTTGCGAGGTCCAGAGGTGGTGAGTGTGAATGGCAGTGGTGGTAATTTGGGGGGAAACAAGGGGGAGGAAGGGAAGAGGGATATGGGTTCAGATGAGGAGGAGGAGGATGAGAAGAAGAAGAAGAAGAAGAAGAGGAGGAGGAAGAGAGATGAGGGGGAAAAAGATGGAGACTTGAGTGCAGAGGAAAATGGGGTTGTGAAGAAGGAGTGTAATAAGCCAAAGAAGAAGAATAAAAAGAAGAAGAAGAAGAGGAAGACGGAGGCGAAAACCGAGGAAGAGAATAAGGGCGGTGAGCTTGAGAATGGAGAGCAAATTGTCCAGGAGACAGATATGAACATTGACAGGCAAGCGAGTGGGGATGCTCTGACAAAAGTTTATGTGGGAGGCATTCCTTATTACTCGACCGAGGATGATATTCGAAGTTACTTTGAAAGCTGTGGTACAATAACTGAAGTTGATTGTCTGAGGTTTCCTGACAGTGGGAAGTTTAGAGGAATTGCTATTATTAGCTTTAAGACAGAAGCAGCAGCCAAGCGAGCCTTGGCTTTTGATGGAGCTGAAATGGGTGGACTGTTTCTGAAAATCCAGCCTTACAAGGCAACTCGACCCAATAAACCGAATAAAGTATCTGATTTTGCCCCACAAATTGTGGAGGGATACAATAAAATCTATGTTGGAAATTTGTCATGGGATATTACTGAGGATGATATCAGGAAGCTTTTCTCAGATTGCAAGATTTCATCTATACATTTTGGTAAGGATAAGGAAACAGGGGAATTTCGGGGTTATGCTCATGTAAATTTCTCTGATAGCCTCTCGGTGACTATGGCATTGAAGTTAGATCAGAAGGTTGTATGTGGAAGACCTGTCAAGATAAGCTGTGCAGTACCTCTGAAAAAAGCAGGGACTCCTTCAAACCTTGCAGCTACAACTTCTAGTACTCATTCAATATCTGTAGCTTCGGCTATGAGCACTGATTCCGTTCCAGTAGATACAAGTACGGGTACAGTAGCTGGTGATGGTGGGTTTAGTGACATCAGTGGTAAGATCAAGAGGAGGACGTGCTATGAGTGTGGTGAGAAGGGTCATCTTTCTTCGGCTTGTCCAAAGTCTGCAACTACAACTTTTAGTACCCATTCAATACCCGTAGCTACAACTACAAGTAGCGATCCTACACCAGCTGCTACATCCTTGGGTACAGGAGCTGATAATAGTGGGTTGAGTGCCACCAGTGGGAAGATTAAGAGGAGGACGTGCTATGAGTGTGGTGAGAAGGGTCATCTTTCTTCAGCTTGTCCAAAGTCTGCAACTACAGCTTTTAATACCCATTCAACACCCGCAGCTAAAACCACAAGTACCGATCCAACACCAGTTGCTACAGCTACAGGAGCTGATAATGGTGGGTTGAGTGACATTAGTGGTAAGATTAAGAGGAGGACCTGCTATGAGTGTGGTGAAAAGGGTCATCTTTCTTCAGCTTGTCCAAAGGCTGCTACTACACCTTCAAGTACCCATTCAATTCGAGTAGCTACAGTTACAAGTACCGGTCTGATTCCAGTAGCTACAACTACAAGCACCGTTAATACACCAGTAGCTACAACAAGAAGCACAGATTTGCCACCAGATGTTACAGCTGCAGGTACTGGAGCTGATCCTAGCGGGTTGTGTGCGAGCAGTGGTAAGATTAAGAGGAGGACGTGCTACGAGTGCGGTGAAAAGGGACATATTTCTTCAGCGTGTCCAAAGAAGCAAACCAATGCAAGTTAA

Protein Analysis

661

Amino Acids

70.37

Weight (kDa)

9.3

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 197 - 264 2.8e-12 RNA recognition motif
RRM_1 PF00076 295 - 362 5.6e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55340
fragaria_vesca FvH4_7g21531
malus_domestica MD01G1122100.v1.1 MD07G1191600.v1.1
prunus_persica Prupe.2G229600_v2.0.a1 Prupe.2G229700_v2.0.a1
pyrus_communis pycom01g14890 pycom07g18100
rosa_chinensis RchiOBHm_Chr1g0365851
rosa_laevigata RLG00000027386
rosa_multiflora Rmu_sc0023137.1_g000001 Rmu_sc0041703.1_g000001
rosa_roxburghii Rroxscaffold_4G00290800
rosa_rugosa Rorug01G0325000
rosa_samantha Rh1AG334000 Rh1BG294600 Rh1CG311300 Rh1DG327000
rosa_wichuraiana Rw1G029630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
Acc36I ACCTGC 2 cut(s) 1664, 1844
AciI CCGC 6 cut(s) 162, 190, 504, 1554, 1875, 1926
AclWI GGATC 4 cut(s) 202, 1379, 1571, 1862
AcsI RAATTY 3 cut(s) 895, 992, 1015
AcuI CTGAAG 5 cut(s) 44, 687, 1488, 1674, 1932
AfiI CCNNNNNNNGG 2 cut(s) 695, 1449
AgeI ACCGGT 1 cut(s) 1760
AgsI TTSAA 8 cut(s) 647, 1051, 1131, 1164, 1356, 1548, 1722, 1734
AjiI CACGTC 3 cut(s) 1278, 1470, 1914
AjnI CCWGG 1 cut(s) 534
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
Alw21I GWGCWC 1 cut(s) 1190
Alw26I GTCTC 2 cut(s) 378, 533
AlwI GGATC 4 cut(s) 202, 1379, 1571, 1862
AlwNI CAGNNNCTG 4 cut(s) 1229, 1421, 1607, 1859
ApeKI GCWGC 7 cut(s) 740, 743, 1139, 1397, 1556, 1706, 1850
ApoI RAATTY 3 cut(s) 895, 992, 1015
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AsiGI ACCGGT 1 cut(s) 1760
Asp700I GAANNNNTTC 1 cut(s) 935
AspS9I GGNCC 2 cut(s) 224, 1653
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 8 cut(s) 247, 518, 1048, 1244, 1304, 1496, 1682, 1940
AsuII TTCGAA 1 cut(s) 634
AsuNHI GCTAGC 1 cut(s) 46
AvaII GGWCC 2 cut(s) 224, 1653
BbsI GAAGAC 2 cut(s) 476, 1084
Bbv12I GWGCWC 1 cut(s) 1190
BbvI GCAGC 7 cut(s) 752, 755, 1151, 1384, 1568, 1693, 1837
BccI CCATC 4 cut(s) 95, 374, 761, 1229
BciT130I CCWGG 1 cut(s) 536
BciVI GTATCC 1 cut(s) 866
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 378, 533
BfaI CTAG 3 cut(s) 47, 1152, 1872
BfmI CTRYAG 6 cut(s) 1170, 1527, 1593, 1599, 1746, 1851
BfuAI ACCTGC 2 cut(s) 1664, 1844
BfuI GTATCC 1 cut(s) 866
BisI GCNGC 7 cut(s) 741, 744, 1140, 1398, 1557, 1707, 1851
BlpI GCTNAGC 1 cut(s) 75
BlsI GCNGC 7 cut(s) 742, 745, 1141, 1399, 1558, 1708, 1852
BmcAI AGTACT 1 cut(s) 1156
Bme1390I CCNGG 2 cut(s) 136, 536
Bme18I GGWCC 2 cut(s) 224, 1653
BmgBI CACGTC 3 cut(s) 1278, 1470, 1914
BmgT120I GGNCC 2 cut(s) 224, 1653
BmrFI CCNGG 2 cut(s) 136, 536
BmsI GCATC 1 cut(s) 565
BmtI GCTAGC 1 cut(s) 50
BpiI GAAGAC 2 cut(s) 476, 1084
BpmI CTGGAG 1 cut(s) 1881
Bpu10I CCTNAGC 1 cut(s) 35
Bpu1102I GCTNAGC 1 cut(s) 75
Bpu14I TTCGAA 1 cut(s) 634
BpuEI CTTGAG 2 cut(s) 409, 533
BpuMI CCSGG 1 cut(s) 136
BsaJI CCNNGG 5 cut(s) 134, 486, 621, 756, 1407
BsaWI WCCGGW 1 cut(s) 1760
Bsc4I CCNNNNNNNGG 2 cut(s) 695, 1449
Bse118I RCCGGY 1 cut(s) 1760
Bse1I ACTGG 6 cut(s) 1202, 1446, 1586, 1772, 1805, 1864
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 5 cut(s) 134, 486, 621, 756, 1407
BseGI GGATG 5 cut(s) 328, 580, 631, 925, 1403
BseLI CCNNNNNNNGG 2 cut(s) 695, 1449
BseMII CTCAG 5 cut(s) 49, 65, 671, 906, 954
BseNI ACTGG 6 cut(s) 1202, 1446, 1586, 1772, 1805, 1864
BseRI GAGGAG 9 cut(s) 326, 329, 332, 364, 367, 1285, 1477, 1663, 1921
BseXI GCAGC 7 cut(s) 752, 755, 1151, 1384, 1568, 1693, 1837
BsgI GTGCAG 2 cut(s) 414, 1119
Bsh1285I CGRYCG 2 cut(s) 140, 621
BshTI ACCGGT 1 cut(s) 1760
BsiEI CGRYCG 2 cut(s) 140, 621
BsiHKAI GWGCWC 1 cut(s) 1190
BsiSI CCGG 2 cut(s) 136, 1761
BslFI GGGAC 2 cut(s) 1135, 1950
BslI CCNNNNNNNGG 2 cut(s) 695, 1449
BsmAI GTCTC 2 cut(s) 378, 533
BsmFI GGGAC 2 cut(s) 1135, 1950
BsmI GAATGC 1 cut(s) 603
Bsp119I TTCGAA 1 cut(s) 634
Bsp1286I GDGCHC 1 cut(s) 1190
Bsp143I GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
Bsp1720I GCTNAGC 1 cut(s) 75
BspACI CCGC 6 cut(s) 162, 190, 504, 1554, 1875, 1926
BspCNI CTCAG 5 cut(s) 48, 64, 672, 907, 953
BspMAI CTGCAG 1 cut(s) 1855
BspMI ACCTGC 2 cut(s) 1664, 1844
BspOI GCTAGC 1 cut(s) 50
BspPI GGATC 4 cut(s) 202, 1379, 1571, 1862
BspT104I TTCGAA 1 cut(s) 634
BsrFI RCCGGY 1 cut(s) 1760
BsrI ACTGG 6 cut(s) 1202, 1446, 1586, 1772, 1805, 1864
BssAI RCCGGY 1 cut(s) 1760
BssECI CCNNGG 5 cut(s) 134, 486, 621, 756, 1407
BssMI GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
BssT1I CCWWGG 2 cut(s) 756, 1407
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 5 cut(s) 695, 789, 1225, 1750, 1795
Bst6I CTCTTC 5 cut(s) 284, 341, 353, 458, 486
BstBI TTCGAA 1 cut(s) 634
BstC8I GCNNGC 4 cut(s) 48, 564, 753, 1888
BstDEI CTNAG 6 cut(s) 35, 51, 75, 680, 915, 940
BstF5I GGATG 5 cut(s) 328, 580, 631, 925, 1403
BstKTI GATC 6 cut(s) 210, 1060, 1266, 1387, 1579, 1870
BstMAI GTCTC 2 cut(s) 378, 533
BstMBI GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
BstMCI CGRYCG 2 cut(s) 140, 621
BstMWI GCNNNNNNNGC 5 cut(s) 148, 752, 1529, 1604, 1923
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 134, 534
BstSFI CTRYAG 6 cut(s) 1170, 1527, 1593, 1599, 1746, 1851
BstV1I GCAGC 7 cut(s) 752, 755, 1151, 1384, 1568, 1693, 1837
BstV2I GAAGAC 2 cut(s) 476, 1084
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BsuI GTATCC 1 cut(s) 866
BtrI CACGTC 3 cut(s) 1278, 1470, 1914
BtsCI GGATG 5 cut(s) 328, 580, 631, 925, 1403
BtsI GCAGTG 2 cut(s) 256, 1897
BtsIMutI CAGTG 6 cut(s) 256, 700, 1188, 1261, 1453, 1897
BveI ACCTGC 2 cut(s) 1664, 1844
Cac8I GCNNGC 4 cut(s) 48, 564, 753, 1888
CaiI CAGNNNCTG 4 cut(s) 1229, 1421, 1607, 1859
Cfr10I RCCGGY 1 cut(s) 1760
Cfr13I GGNCC 2 cut(s) 224, 1653
CspAI ACCGGT 1 cut(s) 1760
CviAII CATG 2 cut(s) 903, 1010
DdeI CTNAG 6 cut(s) 35, 51, 75, 680, 915, 940
DpnI GATC 6 cut(s) 209, 1059, 1265, 1386, 1578, 1869
DpnII GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 5 cut(s) 284, 341, 353, 458, 486
EarI CTCTTC 5 cut(s) 284, 341, 353, 458, 486
Eco130I CCWWGG 2 cut(s) 756, 1407
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 2 cut(s) 224, 1653
Eco57I CTGAAG 5 cut(s) 44, 687, 1488, 1674, 1932
EcoO109I RGGNCCY 1 cut(s) 1653
EcoRII CCWGG 1 cut(s) 534
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 2 cut(s) 756, 1407
ErhI CCWWGG 2 cut(s) 756, 1407
FaeI CATG 2 cut(s) 906, 1013
FalI AAGNNNNNCTT 2 cut(s) 740, 772
FaqI GGGAC 2 cut(s) 1135, 1950
FatI CATG 2 cut(s) 902, 1009
FauI CCCGC 3 cut(s) 183, 1561, 1868
Fnu4HI GCNGC 7 cut(s) 741, 744, 1140, 1398, 1557, 1707, 1851
FokI GGATG 5 cut(s) 335, 587, 638, 932, 1390
Fsp4HI GCNGC 7 cut(s) 741, 744, 1140, 1398, 1557, 1707, 1851
FspBI CTAG 3 cut(s) 47, 1152, 1872
GluI GCNGC 7 cut(s) 741, 744, 1140, 1398, 1557, 1707, 1851
GsuI CTGGAG 1 cut(s) 1881
HapII CCGG 2 cut(s) 136, 1761
Hin1II CATG 2 cut(s) 906, 1013
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 5 cut(s) 65, 158, 1123, 1193, 1768
HpaII CCGG 2 cut(s) 136, 1761
HphI GGTGA 8 cut(s) 247, 518, 1048, 1244, 1304, 1496, 1682, 1940
Hpy166II GTNNAC 1 cut(s) 785
Hpy188III TCNNGA 5 cut(s) 227, 689, 928, 1087, 1267
Hpy8I GTNNAC 1 cut(s) 785
HpyAV CCTTC 7 cut(s) 278, 412, 1057, 1137, 1291, 1483, 1728
HpyCH4III ACNGT 5 cut(s) 695, 789, 1225, 1750, 1795
HpyCH4IV ACGT 3 cut(s) 1277, 1469, 1913
HpyCH4V TGCA 8 cut(s) 395, 948, 1100, 1139, 1331, 1523, 1853, 1976
HpyF10VI GCNNNNNNNGC 5 cut(s) 148, 752, 1529, 1604, 1923
HpyF3I CTNAG 6 cut(s) 35, 51, 75, 680, 915, 940
HpySE526I ACGT 3 cut(s) 1277, 1469, 1913
Hsp92II CATG 2 cut(s) 906, 1013
Kzo9I GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
LmnI GCTCC 4 cut(s) 770, 1418, 1604, 1862
Lsp1109I GCAGC 7 cut(s) 752, 755, 1151, 1384, 1568, 1693, 1837
LweI GCATC 1 cut(s) 565
MaeI CTAG 3 cut(s) 47, 1152, 1872
MaeII ACGT 3 cut(s) 1277, 1469, 1913
MaeIII GTNAC 6 cut(s) 638, 1036, 1247, 1625, 1750, 1843
MalI GATC 6 cut(s) 209, 1059, 1265, 1386, 1578, 1869
MboI GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 1190
MluCI AATT 8 cut(s) 259, 528, 711, 861, 895, 992, 1015, 1734
MlyI GAGTC 2 cut(s) 152, 1117
MmeI TCCRAC 3 cut(s) 87, 871, 1604
MroXI GAANNNNTTC 1 cut(s) 935
MseI TTAA 7 cut(s) 729, 1458, 1536, 1644, 1797, 1902, 1981
MspA1I CMGCKG 2 cut(s) 1397, 1850
MspI CCGG 2 cut(s) 136, 1761
MspR9I CCNGG 2 cut(s) 136, 536
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 5 cut(s) 148, 752, 1529, 1604, 1923
NciI CCSGG 1 cut(s) 136
NdeII GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
NheI GCTAGC 1 cut(s) 46
NlaIII CATG 2 cut(s) 906, 1013
NmuCI GTSAC 3 cut(s) 1036, 1247, 1625
NspV TTCGAA 1 cut(s) 634
PctI GAATGC 1 cut(s) 603
PdmI GAANNNNTTC 1 cut(s) 935
PfeI GAWTC 3 cut(s) 65, 1193, 1768
PfoI TCCNGGA 1 cut(s) 534
PinAI ACCGGT 1 cut(s) 1760
PkrI GCNGC 7 cut(s) 742, 745, 1141, 1399, 1558, 1708, 1852
PleI GAGTC 2 cut(s) 152, 1117
PpsI GAGTC 2 cut(s) 152, 1117
PpuMI RGGWCCY 1 cut(s) 1653
Psp5II RGGWCCY 1 cut(s) 1653
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspPI GGNCC 2 cut(s) 224, 1653
PspPPI RGGWCCY 1 cut(s) 1653
PstI CTGCAG 1 cut(s) 1855
PstNI CAGNNNCTG 4 cut(s) 1229, 1421, 1607, 1859
PsuI RGATCY 1 cut(s) 207
PvuII CAGCTG 2 cut(s) 1397, 1850
SaqAI TTAA 7 cut(s) 729, 1458, 1536, 1644, 1797, 1902, 1981
SatI GCNGC 7 cut(s) 741, 744, 1140, 1398, 1557, 1707, 1851
Sau3AI GATC 6 cut(s) 207, 1057, 1263, 1384, 1576, 1867
Sau96I GGNCC 2 cut(s) 224, 1653
ScaI AGTACT 1 cut(s) 1156
SchI GAGTC 2 cut(s) 152, 1117
ScrFI CCNGG 2 cut(s) 136, 536
SduI GDGCHC 1 cut(s) 1190
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 6 cut(s) 1170, 1527, 1593, 1599, 1746, 1851
SfuI TTCGAA 1 cut(s) 634
SinI GGWCC 2 cut(s) 224, 1653
SmlI CTYRAG 2 cut(s) 388, 512
SmoI CTYRAG 2 cut(s) 388, 512
Sse9I AATT 8 cut(s) 259, 528, 711, 861, 895, 992, 1015, 1734
SsiI CCGC 6 cut(s) 162, 190, 504, 1554, 1875, 1926
SspMI CTAG 3 cut(s) 47, 1152, 1872
StyD4I CCNGG 2 cut(s) 134, 534
StyI CCWWGG 2 cut(s) 756, 1407
TaaI ACNGT 5 cut(s) 695, 789, 1225, 1750, 1795
TaiI ACGT 3 cut(s) 1280, 1472, 1916
TaqI TCGA 5 cut(s) 128, 617, 634, 820, 1738
TaqII GACCGA 1 cut(s) 635
TasI AATT 8 cut(s) 259, 528, 711, 861, 895, 992, 1015, 1734
TatI WGTACW 1 cut(s) 1154
TfiI GAWTC 3 cut(s) 65, 1193, 1768
Tru1I TTAA 7 cut(s) 729, 1458, 1536, 1644, 1797, 1902, 1981
Tru9I TTAA 7 cut(s) 729, 1458, 1536, 1644, 1797, 1902, 1981
TscAI CASTG 6 cut(s) 256, 700, 1195, 1261, 1453, 1897
TseFI GTSAC 3 cut(s) 1036, 1247, 1625
TseI GCWGC 7 cut(s) 740, 743, 1139, 1397, 1556, 1706, 1850
Tsp45I GTSAC 3 cut(s) 1036, 1247, 1625
TspDTI ATGAA 2 cut(s) 563, 945
TspGWI ACGGA 2 cut(s) 488, 1186
TspRI CASTG 6 cut(s) 256, 700, 1195, 1261, 1453, 1897
VpaK11BI GGWCC 2 cut(s) 224, 1653
XapI RAATTY 3 cut(s) 895, 992, 1015
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XmnI GAANNNNTTC 1 cut(s) 935
XspI CTAG 3 cut(s) 47, 1152, 1872
ZrmI AGTACT 1 cut(s) 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.