Rorug01G0325000

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001475
Physical Location & Seq
Reverse (-)
44413168 .. 44423806
10639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0325000.1

Sequence Viewer

Length: 1188 bp
ATGGCGGACTCTCCGGACATAGAGTGGTACTTCTTCAGTCCCCGAGTTTTTAAACACAGCACAAGCAAAGGCAAAGACAAAAGCAAAAGCACTCAAATCAACAGGGCTACCAAGGAAGGCTCCTGGAAGAAGCAAGGCGGTGACAAAAGAATCACTCGGCCCTATTCTGATAAGCAGATCGGGGGGAAAAGGATCTTGACCTTCTACCTGCGGGATAAACAGAAGACTGACTGGGTTATCCATGAGTATTATCTCACCAAGGCCAATTCTGATGAGCAGGTCGGGGACTTTGTTCTCTGCCGCTTGAAGAACAAGTCAGGCAAGTTTGATCATGATCAGCAGGATGATCCACTGTGCGATGGAGGTGAACCTAATAGTGGTAGCTGCAGTATGGTCTCCAATGTTGAAGATCAAGGATCCAAAGAGCTGGGAACTGTTCTTCTGATTTCCAATATCAATGATGATGAAGCTGAACATGGTGGCGGTAGCTGCAGTATGGCCTTCAATGTTGAAAATCAAGCATCCAAACAGCTGGAGAATGTTCTTCCGATTCCCAATCGCAATGATGGTGAAGCTGAACATGGTGGTGGTAGCTGCCTCATCGCCCCTGGTTTCGAAAATGAAGCTGCAGCATTTGGTATGCTTACAGAAGAAGAAATAAACCAGACATTCAAGGAGCTGATAGATGCTGTTTGGGAATATGATGGCAATGAACAACAAGTTAGAGGCTCACAAACTGATTTTTGTTATGTTGATCAGAATGATCTTTGGATCTCTGTTGAAGATCAACCAGCTGATAGCTGCAATTCATCTGATTCCAATATGGTTGAAAAGCCCCTTGATGAGCCAGGAAATCTGGCCTTGCCCCTTCATCCACTTCCGCCACCCAATATGGTCGAAAAGCCACTTGATGAATCTCGTAAGAGGAAACTTTCATTTGGGGATAATGACCCTTTTCTTACGAAGAAGAATCATATTGATGAAGTAGATAGCAACTCTTCATCTAATTCTGAAAATCAAGCTGTGGATACGATTCCAGAGGGATATTCTCAACCAGGAGAAAATCTGGAATCTGATTCATTTCAGTCGCAGCTGGAACTACCATCAATAAACAGAGAACCGGGAGATTTTCCCCATGACAATAATTTAATTGAATGGGAAACACCGAAGTCATCTCTGTATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

395

Amino Acids

44.14

Weight (kDa)

4.55

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 84 1.1e-13 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 216, 268
AccIII TCCGGA 1 cut(s) 13
AciI CCGC 6 cut(s) 5, 138, 211, 301, 483, 881
AclWI GGATC 5 cut(s) 200, 341, 411, 424, 779
AcuI CTGAAG 1 cut(s) 19
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 8 cut(s) 307, 407, 505, 512, 673, 782, 830, 1154
AjnI CCWGG 4 cut(s) 122, 607, 847, 1054
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 5 cut(s) 200, 341, 411, 424, 779
Ama87I CYCGRG 1 cut(s) 42
Aor13HI TCCGGA 1 cut(s) 13
AoxI GGCC 4 cut(s) 158, 261, 498, 858
ApeKI GCWGC 7 cut(s) 384, 489, 594, 626, 629, 801, 1090
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 1122
AsuHPI GGTGA 4 cut(s) 152, 247, 377, 581
AsuII TTCGAA 1 cut(s) 615
AvaI CYCGRG 1 cut(s) 42
BamHI GGATCC 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 230
BbvI GCAGC 7 cut(s) 371, 476, 581, 613, 641, 788, 1102
BccI CCATC 4 cut(s) 353, 560, 698, 1111
BciT130I CCWGG 4 cut(s) 124, 609, 849, 1056
BciVI GTATCC 1 cut(s) 1021
BclI TGATCA 3 cut(s) 328, 334, 754
BcnI CCSGG 1 cut(s) 1122
BcoDI GTCTC 1 cut(s) 400
BfmI CTRYAG 3 cut(s) 385, 490, 627
BfuAI ACCTGC 2 cut(s) 216, 268
BfuI GTATCC 1 cut(s) 1021
BisI GCNGC 8 cut(s) 301, 385, 490, 595, 627, 630, 802, 1091
BlsI GCNGC 8 cut(s) 302, 386, 491, 596, 628, 631, 803, 1092
Bme1390I CCNGG 5 cut(s) 124, 609, 849, 1056, 1122
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 121, 418
BmrFI CCNGG 5 cut(s) 124, 609, 849, 1056, 1122
BmrI ACTGGG 1 cut(s) 241
BmsI GCATC 2 cut(s) 530, 676
BmuI ACTGGG 1 cut(s) 241
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 554
Bpu14I TTCGAA 1 cut(s) 615
BpuMI CCSGG 1 cut(s) 1122
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 3 cut(s) 111, 258, 607
BsaWI WCCGGW 1 cut(s) 13
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 2 cut(s) 568, 715
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 13
BseBI CCWGG 4 cut(s) 124, 609, 849, 1056
BseDI CCNNGG 3 cut(s) 111, 258, 607
BseGI GGATG 3 cut(s) 349, 521, 871
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMI GCAATG 2 cut(s) 568, 715
BseNI ACTGG 1 cut(s) 236
BseXI GCAGC 7 cut(s) 371, 476, 581, 613, 641, 788, 1102
BseYI CCCAGC 1 cut(s) 427
BshFI GGCC 4 cut(s) 160, 263, 500, 860
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 2 cut(s) 14, 1121
BslFI GGGAC 2 cut(s) 24, 299
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 400
BsmFI GGGAC 2 cut(s) 24, 299
BsnI GGCC 4 cut(s) 160, 263, 500, 860
Bso31I GGTCTC 1 cut(s) 400
BsoBI CYCGRG 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 615
Bsp13I TCCGGA 1 cut(s) 13
BspACI CCGC 6 cut(s) 5, 138, 211, 301, 483, 881
BspANI GGCC 4 cut(s) 160, 263, 500, 860
BspEI TCCGGA 1 cut(s) 13
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 121, 418
BspMAI CTGCAG 3 cut(s) 389, 494, 631
BspMI ACCTGC 2 cut(s) 216, 268
BspPI GGATC 5 cut(s) 200, 341, 411, 424, 779
BspT104I TTCGAA 1 cut(s) 615
BspTNI GGTCTC 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 568, 715
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 3 cut(s) 111, 258, 607
BssT1I CCWWGG 2 cut(s) 111, 258
Bst2UI CCWGG 4 cut(s) 124, 609, 849, 1056
Bst4CI ACNGT 2 cut(s) 354, 436
Bst6I CTCTTC 1 cut(s) 1003
BstBI TTCGAA 1 cut(s) 615
BstF5I GGATG 3 cut(s) 349, 521, 871
BstMAI GTCTC 1 cut(s) 400
BstMWI GCNNNNNNNGC 1 cut(s) 489
BstNI CCWGG 4 cut(s) 124, 609, 849, 1056
BstSCI CCNGG 5 cut(s) 122, 607, 847, 1054, 1120
BstSFI CTRYAG 3 cut(s) 385, 490, 627
BstV1I GCAGC 7 cut(s) 371, 476, 581, 613, 641, 788, 1102
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 3 cut(s) 192, 416, 771
BstXI CCANNNNNNTGG 2 cut(s) 427, 532
BstYI RGATCY 3 cut(s) 192, 416, 771
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 4 cut(s) 160, 263, 500, 860
BtgZI GCGATG 2 cut(s) 372, 586
BtsCI GGATG 3 cut(s) 349, 521, 871
BtsIMutI CAGTG 1 cut(s) 350
BveI ACCTGC 2 cut(s) 216, 268
CciI TCATGA 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 28
CviAII CATG 5 cut(s) 242, 332, 476, 581, 1136
CviQI GTAC 1 cut(s) 28
DraI TTTAAA 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 1003
EarI CTCTTC 1 cut(s) 1003
EciI GGCGGA 2 cut(s) 20, 870
Eco130I CCWWGG 2 cut(s) 111, 258
Eco31I GGTCTC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 42
EcoRII CCWGG 4 cut(s) 122, 607, 847, 1054
EcoT14I CCWWGG 2 cut(s) 111, 258
ErhI CCWWGG 2 cut(s) 111, 258
FaeI CATG 5 cut(s) 245, 335, 479, 584, 1139
FaqI GGGAC 2 cut(s) 24, 299
FatI CATG 5 cut(s) 241, 331, 475, 580, 1135
FauI CCCGC 1 cut(s) 204
FbaI TGATCA 3 cut(s) 328, 334, 754
Fnu4HI GCNGC 8 cut(s) 301, 385, 490, 595, 627, 630, 802, 1091
FokI GGATG 3 cut(s) 356, 508, 858
Fsp4HI GCNGC 8 cut(s) 301, 385, 490, 595, 627, 630, 802, 1091
GluI GCNGC 8 cut(s) 301, 385, 490, 595, 627, 630, 802, 1091
GsaI CCCAGC 1 cut(s) 431
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 4 cut(s) 160, 263, 500, 860
HapII CCGG 2 cut(s) 14, 1121
Hin1II CATG 5 cut(s) 245, 335, 479, 584, 1139
HinfI GANTC 9 cut(s) 8, 150, 550, 815, 914, 970, 1033, 1070, 1076
HpaII CCGG 2 cut(s) 14, 1121
HphI GGTGA 4 cut(s) 152, 247, 377, 581
Hpy166II GTNNAC 1 cut(s) 368
Hpy188I TCNGA 8 cut(s) 169, 271, 444, 549, 759, 814, 1012, 1075
Hpy188III TCNNGA 5 cut(s) 14, 196, 332, 1037, 1067
Hpy8I GTNNAC 1 cut(s) 368
HpyAV CCTTC 4 cut(s) 110, 211, 511, 878
HpyCH4III ACNGT 2 cut(s) 354, 436
HpyCH4V TGCA 4 cut(s) 387, 492, 629, 804
HpyF10VI GCNNNNNNNGC 1 cut(s) 489
Hsp92II CATG 5 cut(s) 245, 335, 479, 584, 1139
Kpn2I TCCGGA 1 cut(s) 13
Ksp22I TGATCA 3 cut(s) 328, 334, 754
LmnI GCTCC 2 cut(s) 125, 676
Lsp1109I GCAGC 7 cut(s) 371, 476, 581, 613, 641, 788, 1102
LweI GCATC 2 cut(s) 530, 676
MaeIII GTNAC 1 cut(s) 140
MflI RGATCY 3 cut(s) 192, 416, 771
MluCI AATT 5 cut(s) 265, 805, 1006, 1144, 1149
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 5 cut(s) 356, 608, 719, 918, 1033
MroI TCCGGA 1 cut(s) 13
MseI TTAA 2 cut(s) 51, 1148
MslI CAYNNNNRTG 2 cut(s) 585, 978
MspA1I CMGCKG 3 cut(s) 532, 794, 1093
MspI CCGG 2 cut(s) 14, 1121
MspR9I CCNGG 5 cut(s) 124, 609, 849, 1056, 1122
MvaI CCWGG 4 cut(s) 124, 609, 849, 1056
MwoI GCNNNNNNNGC 1 cut(s) 489
NciI CCSGG 1 cut(s) 1122
NlaIII CATG 5 cut(s) 245, 335, 479, 584, 1139
NlaIV GGNNCC 2 cut(s) 121, 418
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 140
NspV TTCGAA 1 cut(s) 615
PagI TCATGA 1 cut(s) 331
PfeI GAWTC 8 cut(s) 150, 550, 815, 914, 970, 1033, 1070, 1076
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 8 cut(s) 302, 386, 491, 596, 628, 631, 803, 1092
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Psp6I CCWGG 4 cut(s) 122, 607, 847, 1054
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 4 cut(s) 122, 607, 847, 1054
PspN4I GGNNCC 2 cut(s) 121, 418
PspPI GGNCC 1 cut(s) 159
PstI CTGCAG 3 cut(s) 389, 494, 631
PsuI RGATCY 3 cut(s) 192, 416, 771
PvuII CAGCTG 3 cut(s) 532, 794, 1093
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 2 cut(s) 585, 978
SaqAI TTAA 2 cut(s) 51, 1148
SatI GCNGC 8 cut(s) 301, 385, 490, 595, 627, 630, 802, 1091
Sau96I GGNCC 1 cut(s) 159
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 5 cut(s) 124, 609, 849, 1056, 1122
SfaNI GCATC 2 cut(s) 530, 676
SfcI CTRYAG 3 cut(s) 385, 490, 627
SfuI TTCGAA 1 cut(s) 615
SmiMI CAYNNNNRTG 2 cut(s) 585, 978
Sse9I AATT 5 cut(s) 265, 805, 1006, 1144, 1149
SsiI CCGC 6 cut(s) 5, 138, 211, 301, 483, 881
StyD4I CCNGG 5 cut(s) 122, 607, 847, 1054, 1120
StyI CCWWGG 2 cut(s) 111, 258
TaaI ACNGT 2 cut(s) 354, 436
TaqI TCGA 2 cut(s) 615, 897
TasI AATT 5 cut(s) 265, 805, 1006, 1144, 1149
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 8 cut(s) 150, 550, 815, 914, 970, 1033, 1070, 1076
Tru1I TTAA 2 cut(s) 51, 1148
Tru9I TTAA 2 cut(s) 51, 1148
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 7 cut(s) 384, 489, 594, 626, 629, 801, 1090
Tsp45I GTSAC 1 cut(s) 140
TspRI CASTG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.