Rmu_co8241371.1_g000001

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8241371.1
OG0001475
Physical Location & Seq
Reverse (-)
58 .. 659
602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8241371.1_g000001.1.cds

Sequence Viewer

Length: 486 bp
atgtatggtgtttctgtaaaatatggtgttactaagataatttcttgcaattcacaggccgatgcagcatctggtattgctgtgcacaatgagtgcaagttgaagtttctggagctcaagacaaagaggacctaccgttcaatagtcttcaagattgaggagaagcaaaagcaagttattgtggaagcaactggtgatccaacagaaacatatgaggctttcacagatagtctgcctactgatgaatgctgttatgctgtttttgattttgatcttcctataccagagggtgtcccaaaaaggcagatattgtttattgcctggttgcctgatacatcaagggaggaaaacaagaagatctacgcaagcgcgttggacagttttaagagagagcttgatgggattcaaatcgagttggaggcaactggtccatctgagatcggccttgatgtaattaggaaccgtgcagctaaacatgaaccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

161

Amino Acids

18.09

Weight (kDa)

5.17

Isoelectric Point (pI)

53.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 371
AclWI GGATC 1 cut(s) 191
AgsI TTSAA 4 cut(s) 103, 141, 151, 407
AjnI CCWGG 1 cut(s) 320
AloI GAACNNNNNNTCC 2 cut(s) 121, 153
AluBI AGCT 3 cut(s) 115, 394, 470
AluI AGCT 3 cut(s) 115, 394, 470
Alw21I GWGCWC 2 cut(s) 87, 117
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 2 cut(s) 57, 442
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 2 cut(s) 65, 467
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 129, 428
AsuHPI GGTGA 1 cut(s) 206
AvaII GGWCC 2 cut(s) 129, 428
BaeGI GKGCMC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 2 cut(s) 87, 117
BbvI GCAGC 2 cut(s) 77, 479
BccI CCATC 2 cut(s) 392, 439
BciT130I CCWGG 1 cut(s) 322
BglII AGATCT 1 cut(s) 357
BisI GCNGC 2 cut(s) 66, 468
BlsI GCNGC 2 cut(s) 67, 469
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 2 cut(s) 129, 428
BmgT120I GGNCC 2 cut(s) 129, 428
BmiI GGNNCC 1 cut(s) 461
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 2 cut(s) 52, 77
BpiI GAAGAC 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 2 cut(s) 270, 407
Bse1I ACTGG 2 cut(s) 196, 430
Bse8I GATNNNNATC 2 cut(s) 270, 407
BseBI CCWGG 1 cut(s) 322
BseJI GATNNNNATC 2 cut(s) 270, 407
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 196, 430
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 2 cut(s) 77, 479
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 371
BshFI GGCC 2 cut(s) 59, 444
BsiHKAI GWGCWC 2 cut(s) 87, 117
BslFI GGGAC 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 2 cut(s) 59, 444
Bsp1286I GDGCHC 2 cut(s) 87, 117
Bsp143I GATC 4 cut(s) 196, 271, 357, 438
BspANI GGCC 2 cut(s) 59, 444
BspCNI CTCAG 1 cut(s) 427
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 1 cut(s) 461
BspPI GGATC 1 cut(s) 191
BsrI ACTGG 2 cut(s) 196, 430
BssMI GATC 4 cut(s) 196, 271, 357, 438
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 3 cut(s) 137, 380, 464
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 2 cut(s) 33, 435
BstFNI CGCG 1 cut(s) 371
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 4 cut(s) 199, 274, 360, 441
BstMBI GATC 4 cut(s) 196, 271, 357, 438
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 87
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 2 cut(s) 77, 479
BstV2I GAAGAC 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 357
BstYI RGATCY 1 cut(s) 357
BsuRI GGCC 2 cut(s) 59, 444
Cac8I GCNNGC 1 cut(s) 367
CaiI CAGNNNCTG 1 cut(s) 71
CfoI GCGC 1 cut(s) 371
Cfr13I GGNCC 2 cut(s) 129, 428
CviAII CATG 1 cut(s) 476
CviJI RGCY 6 cut(s) 59, 115, 218, 394, 444, 470
CviKI_1 RGCY 6 cut(s) 59, 115, 218, 394, 444, 470
DdeI CTNAG 2 cut(s) 33, 435
DpnI GATC 4 cut(s) 198, 273, 359, 440
DpnII GATC 4 cut(s) 196, 271, 357, 438
Ecl136II GAGCTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 129, 428
Eco53kI GAGCTC 1 cut(s) 115
EcoICRI GAGCTC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 117
FaeI CATG 1 cut(s) 479
FaiI YATR 8 cut(s) 6, 24, 211, 213, 255, 281, 477, 484
FaqI GGGAC 1 cut(s) 278
FatI CATG 1 cut(s) 475
FauNDI CATATG 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 66, 468
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 2 cut(s) 66, 468
GlaI GCGC 1 cut(s) 370
GluI GCNGC 2 cut(s) 66, 468
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 2 cut(s) 59, 444
HhaI GCGC 1 cut(s) 371
Hin1II CATG 1 cut(s) 479
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HinfI GANTC 1 cut(s) 403
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 1 cut(s) 436
Hpy188III TCNNGA 3 cut(s) 110, 118, 151
Hpy8I GTNNAC 1 cut(s) 85
HpyCH4III ACNGT 3 cut(s) 137, 380, 464
HpyCH4V TGCA 5 cut(s) 48, 65, 85, 96, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 2 cut(s) 33, 435
Hsp92II CATG 1 cut(s) 479
HspAI GCGC 1 cut(s) 369
Kzo9I GATC 4 cut(s) 196, 271, 357, 438
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 9 cut(s) 41, 57, 95, 177, 297, 307, 334, 342, 411
Lsp1109I GCAGC 2 cut(s) 77, 479
LweI GCATC 2 cut(s) 52, 77
MaeIII GTNAC 1 cut(s) 28
MalI GATC 4 cut(s) 198, 273, 359, 440
MboI GATC 4 cut(s) 196, 271, 357, 438
MboII GAAGA 3 cut(s) 139, 266, 367
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 2 cut(s) 87, 117
MluCI AATT 3 cut(s) 39, 49, 453
MmeI TCCRAC 3 cut(s) 224, 354, 396
MnlI CCTC 6 cut(s) 120, 151, 208, 280, 337, 412
MseI TTAA 1 cut(s) 384
MspR9I CCNGG 1 cut(s) 322
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeI CATATG 1 cut(s) 211
NdeII GATC 4 cut(s) 196, 271, 357, 438
NlaIII CATG 1 cut(s) 479
NlaIV GGNNCC 1 cut(s) 461
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 403
PkrI GCNGC 2 cut(s) 67, 469
PpuMI RGGWCCY 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 461
PspPI GGNCC 2 cut(s) 129, 428
PspPPI RGGWCCY 1 cut(s) 129
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 357
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 66, 468
Sau3AI GATC 4 cut(s) 196, 271, 357, 438
Sau96I GGNCC 2 cut(s) 129, 428
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 2 cut(s) 87, 117
SetI ASST 4 cut(s) 117, 134, 396, 472
SfaNI GCATC 2 cut(s) 52, 77
SinI GGWCC 2 cut(s) 129, 428
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 3 cut(s) 39, 49, 453
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 3 cut(s) 137, 380, 464
TaqI TCGA 1 cut(s) 411
TasI AATT 3 cut(s) 39, 49, 453
TfiI GAWTC 1 cut(s) 403
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseI GCWGC 2 cut(s) 65, 467
TspDTI ATGAA 1 cut(s) 258
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 2 cut(s) 129, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.