MD01G1122300.v1.1

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001475
Physical Location & Seq
Reverse (-)
23561774 .. 23564008
2235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1122300.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGCGAACGCACGATCAGGGATGGCTGTACACGACGAGTGCAAGATAAAATTTTTAGAATTGAAGGCAAAAAGAACTTACCGCTTCATAGTTTACAAGATCGAGGAGAAGCAAAATGAGGTCATAGTGGAAAAGCTTGGCCAACCAACTGACAGCTATGAGGATTTCTCTGCAAGCCTCCCTGCTAACGAGTGCCGATACGCTGTTTATGACTTTGATTACGTGACGGAGGAGAACTGCCGGAAGAGCCGGATTATTTTCGTTGCTTGGTCCCCTGACACAGCGAAGGTGAGAAGCAAGATGATTTACGCGAGTTCAAAGGACACGTTTAAGAGAGAGTTGGACGGAATTCAGATGGAGTTGCAGGCTACTGATCCTACCGAAGTGGGTCTCGATGTTATTAGAAGCCGTGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

140

Amino Acids

16.06

Weight (kDa)

5.93

Isoelectric Point (pI)

55.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 138 5.8e-42 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 311
AciI CCGC 1 cut(s) 82
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 2 cut(s) 50, 348
AfaI GTAC 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 2 cut(s) 64, 318
AluBI AGCT 2 cut(s) 136, 156
AluI AGCT 2 cut(s) 136, 156
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 368
AoxI GGCC 1 cut(s) 139
ApoI RAATTY 2 cut(s) 50, 348
AspS9I GGNCC 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 1 cut(s) 270
BalI TGGCCA 1 cut(s) 141
BccI CCATC 2 cut(s) 16, 349
BceAI ACGGC 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 395
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 272
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BsaAI YACGTR 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 395
BseGI GGATG 1 cut(s) 27
BseRI GAGGAG 2 cut(s) 119, 245
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 141
BsiSI CCGG 2 cut(s) 241, 250
BslFI GGGAC 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 395
Bsp1407I TGTACA 1 cut(s) 28
Bsp143I GATC 3 cut(s) 14, 99, 373
BspACI CCGC 1 cut(s) 82
BspANI GGCC 1 cut(s) 141
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 1 cut(s) 272
BspPI GGATC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 395
BsrGI TGTACA 1 cut(s) 28
BssMI GATC 3 cut(s) 14, 99, 373
Bst6I CTCTTC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 28
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 175, 366
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 311
BstKTI GATC 3 cut(s) 17, 102, 376
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 3 cut(s) 14, 99, 373
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstUI CGCG 1 cut(s) 311
BsuRI GGCC 1 cut(s) 141
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 175, 366
Cfr13I GGNCC 1 cut(s) 270
Csp6I GTAC 1 cut(s) 29
CviJI RGCY 8 cut(s) 26, 136, 141, 156, 177, 249, 368, 408
CviKI_1 RGCY 8 cut(s) 26, 136, 141, 156, 177, 249, 368, 408
CviQI GTAC 1 cut(s) 29
DpnI GATC 3 cut(s) 16, 101, 375
DpnII GATC 3 cut(s) 14, 99, 373
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 348
FaiI YATR 4 cut(s) 89, 125, 159, 210
FaqI GGGAC 1 cut(s) 256
FokI GGATG 1 cut(s) 34
HaeIII GGCC 1 cut(s) 141
HapII CCGG 2 cut(s) 241, 250
HindIII AAGCTT 1 cut(s) 134
HpaII CCGG 2 cut(s) 241, 250
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 31, 94
Hpy188I TCNGA 1 cut(s) 354
Hpy188III TCNNGA 1 cut(s) 392
Hpy8I GTNNAC 2 cut(s) 31, 94
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 58, 280
HpyCH4IV ACGT 2 cut(s) 222, 326
HpyCH4V TGCA 3 cut(s) 42, 173, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpySE526I ACGT 2 cut(s) 222, 326
Kzo9I GATC 3 cut(s) 14, 99, 373
LguI GCTCTTC 1 cut(s) 239
LpnPI CCDG 6 cut(s) 3, 195, 254, 263, 288, 350
MaeII ACGT 2 cut(s) 222, 326
MaeIII GTNAC 1 cut(s) 223
MalI GATC 3 cut(s) 16, 101, 375
MboI GATC 3 cut(s) 14, 99, 373
MboII GAAGA 1 cut(s) 256
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 4 cut(s) 50, 59, 348, 415
MluNI TGGCCA 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 5 cut(s) 97, 112, 154, 188, 223
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 141
MspI CCGG 2 cut(s) 241, 250
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 3 cut(s) 14, 99, 373
NlaIV GGNNCC 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 239
Ppu21I YACGTR 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 270
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 3 cut(s) 14, 99, 373
Sau96I GGNCC 1 cut(s) 270
SetI ASST 6 cut(s) 123, 138, 158, 225, 291, 329
SinI GGWCC 1 cut(s) 270
Sse9I AATT 4 cut(s) 50, 59, 348, 415
SsiI CCGC 1 cut(s) 82
TaiI ACGT 2 cut(s) 225, 329
TaqI TCGA 2 cut(s) 102, 393
TasI AATT 4 cut(s) 50, 59, 348, 415
TatI WGTACW 1 cut(s) 28
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 76
TspGWI ACGGA 2 cut(s) 242, 360
VpaK11BI GGWCC 1 cut(s) 270
XapI RAATTY 2 cut(s) 50, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.