RLG00000024518

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001475
Physical Location & Seq
Forward (+)
36641268 .. 36642780
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024518

Sequence Viewer

Length: 420 bp
ATGGCCAATGCAGCGTCCGGTATGGCTGTGCACGATGAGTGCAAATTGAAGTTTCTGGAGCTCAAGACAAAGAGGACCTACCGATCAATAGTGTACAAGATCGAGGAGAAGCAAAAGCAAGTTATTGTGGAAGCAACTGGGGATCCAACACAAACCTATGAGAATTTCACAGATAGCCTTCCTTCTGATGAATGCCGTTATGCTGTTTTTGATTTCGATTTTCTTACACCAGAGGGTGTCCCAAAAAGCAGGATATTCTTCATTGCCTGGTCTCCTGATACATCAAGGGTGAGAAACAAGATGATCTATGCAAGCTCAAAGGACAGATTCAAGAGAGAGCTTGATGGAATTCAGGTCGAGTTGCAGGCAACTGATCCATCTGAGATTGGCCTTGATGTCATTAAGAGCCGTGCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

140

Amino Acids

15.9

Weight (kDa)

5.9

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 138 6.4e-39 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 137, 150, 368
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 163, 348
AfaI GTAC 1 cut(s) 95
AgsI TTSAA 2 cut(s) 49, 331
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 4 cut(s) 61, 315, 340, 417
AluI AGCT 4 cut(s) 61, 315, 340, 417
Alw21I GWGCWC 2 cut(s) 33, 63
Alw26I GTCTC 1 cut(s) 276
Alw44I GTGCAC 1 cut(s) 29
AlwI GGATC 3 cut(s) 137, 150, 368
AoxI GGCC 2 cut(s) 3, 388
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 2 cut(s) 163, 348
AspS9I GGNCC 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 1 cut(s) 75
BaeGI GKGCMC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 33, 63
BbvI GCAGC 1 cut(s) 23
BccI CCATC 2 cut(s) 338, 385
BceAI ACGGC 2 cut(s) 180, 393
BciT130I CCWGG 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 276
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 268
BmrI ACTGGG 1 cut(s) 147
BmuI ACTGGG 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 142
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 268
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 2 cut(s) 5, 390
BsiHKAI GWGCWC 2 cut(s) 33, 63
BsiSI CCGG 1 cut(s) 18
BslFI GGGAC 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 2 cut(s) 5, 390
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 33, 63
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 5 cut(s) 83, 99, 142, 303, 373
BspANI GGCC 2 cut(s) 5, 390
BspCNI CTCAG 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 144
BspPI GGATC 3 cut(s) 137, 150, 368
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 261
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 1 cut(s) 142
BssMI GATC 5 cut(s) 83, 99, 142, 303, 373
Bst2UI CCWGG 1 cut(s) 268
BstAUI TGTACA 1 cut(s) 93
BstC8I GCNNGC 3 cut(s) 313, 366, 415
BstDEI CTNAG 1 cut(s) 381
BstKTI GATC 5 cut(s) 86, 102, 145, 306, 376
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 5 cut(s) 83, 99, 142, 303, 373
BstMWI GCNNNNNNNGC 2 cut(s) 11, 414
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 266
BstSLI GKGCMC 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuRI GGCC 2 cut(s) 5, 390
Cac8I GCNNGC 3 cut(s) 313, 366, 415
Cfr13I GGNCC 1 cut(s) 75
CseI GACGC 1 cut(s) 3
Csp6I GTAC 1 cut(s) 94
CviJI RGCY 9 cut(s) 5, 26, 61, 177, 315, 340, 390, 408, 417
CviKI_1 RGCY 9 cut(s) 5, 26, 61, 177, 315, 340, 390, 408, 417
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 381
DpnI GATC 5 cut(s) 85, 101, 144, 305, 375
DpnII GATC 5 cut(s) 83, 99, 142, 303, 373
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 61
EcoICRI GAGCTC 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 75
EcoRI GAATTC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 266
EcoT38I GRGCYC 1 cut(s) 63
FaiI YATR 4 cut(s) 23, 159, 201, 309
FaqI GGGAC 1 cut(s) 224
Fnu4HI GCNGC 1 cut(s) 12
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 12
GluI GCNGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 2 cut(s) 5, 390
HapII CCGG 1 cut(s) 18
HgaI GACGC 1 cut(s) 3
HinfI GANTC 1 cut(s) 327
HpaII CCGG 1 cut(s) 18
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 31, 94
Hpy188I TCNGA 2 cut(s) 187, 382
Hpy188III TCNNGA 4 cut(s) 56, 64, 275, 331
Hpy8I GTNNAC 2 cut(s) 31, 94
HpyAV CCTTC 2 cut(s) 188, 192
HpyCH4V TGCA 5 cut(s) 11, 31, 42, 311, 364
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 414
HpyF3I CTNAG 1 cut(s) 381
Kzo9I GATC 5 cut(s) 83, 99, 142, 303, 373
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 1 cut(s) 23
MalI GATC 5 cut(s) 85, 101, 144, 305, 375
MboI GATC 5 cut(s) 83, 99, 142, 303, 373
MboII GAAGA 1 cut(s) 250
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 2 cut(s) 33, 63
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 44, 163, 348
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 3 cut(s) 66, 97, 226
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 268
MwoI GCNNNNNNNGC 2 cut(s) 11, 414
NdeII GATC 5 cut(s) 83, 99, 142, 303, 373
NlaIV GGNNCC 1 cut(s) 144
PctI GAATGC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 327
PkrI GCNGC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 75
PspPPI RGGWCCY 1 cut(s) 75
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 5 cut(s) 83, 99, 142, 303, 373
Sau96I GGNCC 1 cut(s) 75
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 2 cut(s) 33, 63
SetI ASST 7 cut(s) 63, 80, 158, 317, 342, 357, 419
SinI GGWCC 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 3 cut(s) 44, 163, 348
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 266
TaqI TCGA 3 cut(s) 102, 216, 357
TasI AATT 3 cut(s) 44, 163, 348
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 1 cut(s) 327
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 2 cut(s) 204, 250
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 2 cut(s) 163, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.