Rmu_sc0004404.1_g000004

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004404.1
OG0001475
Physical Location & Seq
Reverse (-)
16645 .. 18449
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004404.1_g000004.1.cds

Sequence Viewer

Length: 1041 bp
atgtcatatgcaaagaagttgatcgaaatgggggcagggcgttactggacaactgaagacgataagaaactgaaatatgcagtgaagaaagttgctgctcgtaagggaggacacatcaaagattggaagaaagtggctttcatagtcggaaaaaggggagacctctgcaaagacagatggcagaatgtggtttctccagatataaagaaaggagcttggagcaaggaggaggatcaaaggctcctcaatgcctctgcttcttgtgattcaaacatgttgaatactgtgcttgatgtcttcaattcttcttctgtggatggaaagatcagaacactccatcaatgccaggctcgactacaacaacttttgaatggaaaccattcccggaagcttgaagaaaagtcggtagaacaggggaatattgcaggattatatcagcagaaagctgaaggagtgggtgatgaaggagtggctgatgaaggggtggttgatgaaggagtggcttatgcacaagttgctgatgctttaaccctattgcaaaataactataatggagaagaagaattggagataggtgatggcttaaccaatcttttcaaaacttatgcagaggccgatgcagcatctggtattgctgtgcacaatgagtgcaagttgaagtttctggagctcaagacaaagaggacctaccgttcaatagtcttcaagattgaggagaagcaaaagcaagttattgtggaagcaactggtgatccaacagaaacatatgaggctttcacagatagtctgcctactgatgaatgctgttatgctgtttttgattttgatcttcctataccagagggtgtcccaaaaaggcagatattgtttattgcctggttgcctgatacatcaagggaggaaaacaagaagatctacgcaagcgcgttggacagttttaagagagagcttgatgggattcaaatcgagttggaggcaactggtccatctgagatcggccttgatgtaattaggaaccgtgcagctaaacatgaaccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

346

Amino Acids

38.64

Weight (kDa)

5.33

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 926
AclWI GGATC 2 cut(s) 240, 746
AcuI CTGAAG 2 cut(s) 75, 468
AflIII ACRYGT 1 cut(s) 273
AjnI CCWGG 2 cut(s) 345, 875
AloI GAACNNNNNNTCC 2 cut(s) 676, 708
AluBI AGCT 6 cut(s) 215, 391, 446, 670, 949, 1025
AluI AGCT 6 cut(s) 215, 391, 446, 670, 949, 1025
Alw21I GWGCWC 2 cut(s) 642, 672
Alw26I GTCTC 1 cut(s) 153
Alw44I GTGCAC 1 cut(s) 638
AlwI GGATC 2 cut(s) 240, 746
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 2 cut(s) 612, 997
ApaLI GTGCAC 1 cut(s) 638
ApeKI GCWGC 3 cut(s) 95, 620, 1022
Asp700I GAANNNNTTC 1 cut(s) 379
AspLEI GCGC 1 cut(s) 926
AspS9I GGNCC 2 cut(s) 684, 983
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 3 cut(s) 470, 587, 761
AvaII GGWCC 2 cut(s) 684, 983
BaeGI GKGCMC 1 cut(s) 642
BanII GRGCYC 1 cut(s) 672
BbsI GAAGAC 3 cut(s) 63, 289, 694
Bbv12I GWGCWC 2 cut(s) 642, 672
BbvI GCAGC 3 cut(s) 82, 632, 1034
BccI CCATC 6 cut(s) 171, 311, 345, 572, 947, 994
BciT130I CCWGG 2 cut(s) 347, 877
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 153
BglII AGATCT 1 cut(s) 912
BisI GCNGC 3 cut(s) 96, 621, 1023
BlsI GCNGC 3 cut(s) 97, 622, 1024
Bme1390I CCNGG 3 cut(s) 347, 385, 877
Bme18I GGWCC 2 cut(s) 684, 983
BmgT120I GGNCC 2 cut(s) 684, 983
BmiI GGNNCC 2 cut(s) 242, 1016
BmrFI CCNGG 3 cut(s) 347, 385, 877
BmsI GCATC 3 cut(s) 511, 607, 632
BpiI GAAGAC 3 cut(s) 63, 289, 694
BpmI CTGGAG 2 cut(s) 180, 686
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 1 cut(s) 385
BsaBI GATNNNNATC 2 cut(s) 825, 962
BsaI GGTCTC 1 cut(s) 153
Bse1I ACTGG 3 cut(s) 50, 751, 985
Bse8I GATNNNNATC 2 cut(s) 825, 962
BseBI CCWGG 2 cut(s) 347, 877
BseGI GGATG 1 cut(s) 322
BseJI GATNNNNATC 2 cut(s) 825, 962
BseMII CTCAG 1 cut(s) 981
BseNI ACTGG 3 cut(s) 50, 751, 985
BseRI GAGGAG 3 cut(s) 233, 242, 728
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 3 cut(s) 82, 632, 1034
BsgI GTGCAG 1 cut(s) 1041
Bsh1236I CGCG 1 cut(s) 926
BshFI GGCC 2 cut(s) 614, 999
BsiHKAI GWGCWC 2 cut(s) 642, 672
BsiSI CCGG 1 cut(s) 385
BslFI GGGAC 1 cut(s) 833
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 833
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 2 cut(s) 614, 999
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 642, 672
Bsp143I GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
BspANI GGCC 2 cut(s) 614, 999
BspCNI CTCAG 1 cut(s) 982
BspFNI CGCG 1 cut(s) 926
BspLI GGNNCC 2 cut(s) 242, 1016
BspPI GGATC 2 cut(s) 240, 746
BspTNI GGTCTC 1 cut(s) 153
BsrI ACTGG 3 cut(s) 50, 751, 985
BssMI GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
Bst2UI CCWGG 2 cut(s) 347, 877
Bst4CI ACNGT 4 cut(s) 286, 692, 935, 1019
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 922
BstDEI CTNAG 1 cut(s) 990
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 1 cut(s) 926
BstHHI GCGC 1 cut(s) 926
BstKTI GATC 7 cut(s) 24, 235, 327, 754, 829, 915, 996
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
BstMWI GCNNNNNNNGC 2 cut(s) 515, 620
BstNI CCWGG 2 cut(s) 347, 877
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 3 cut(s) 345, 383, 875
BstSLI GKGCMC 1 cut(s) 642
BstUI CGCG 1 cut(s) 926
BstV1I GCAGC 3 cut(s) 82, 632, 1034
BstV2I GAAGAC 3 cut(s) 63, 289, 694
BstX2I RGATCY 1 cut(s) 912
BstYI RGATCY 1 cut(s) 912
BsuRI GGCC 2 cut(s) 614, 999
BtsCI GGATG 1 cut(s) 322
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 922
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 1 cut(s) 926
Cfr13I GGNCC 2 cut(s) 684, 983
CviAII CATG 2 cut(s) 274, 1031
DdeI CTNAG 1 cut(s) 990
DpnI GATC 7 cut(s) 23, 234, 326, 753, 828, 914, 995
DpnII GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
Ecl136II GAGCTC 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 672
Eco31I GGTCTC 1 cut(s) 153
Eco47I GGWCC 2 cut(s) 684, 983
Eco53kI GAGCTC 1 cut(s) 670
Eco57I CTGAAG 2 cut(s) 75, 468
EcoICRI GAGCTC 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 684
EcoRII CCWGG 2 cut(s) 345, 875
EcoT38I GRGCYC 1 cut(s) 672
FaeI CATG 2 cut(s) 277, 1034
FaqI GGGAC 1 cut(s) 833
FatI CATG 2 cut(s) 273, 1030
FauNDI CATATG 2 cut(s) 7, 766
Fnu4HI GCNGC 3 cut(s) 96, 621, 1023
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 3 cut(s) 96, 621, 1023
GlaI GCGC 1 cut(s) 925
GluI GCNGC 3 cut(s) 96, 621, 1023
GsuI CTGGAG 2 cut(s) 180, 686
HaeIII GGCC 2 cut(s) 614, 999
HapII CCGG 1 cut(s) 385
HhaI GCGC 1 cut(s) 926
Hin1II CATG 2 cut(s) 277, 1034
Hin6I GCGC 1 cut(s) 924
HinP1I GCGC 1 cut(s) 924
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 2 cut(s) 266, 958
HpaII CCGG 1 cut(s) 385
HphI GGTGA 3 cut(s) 470, 587, 761
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 3 cut(s) 149, 329, 991
Hpy188III TCNNGA 4 cut(s) 197, 665, 673, 706
Hpy8I GTNNAC 1 cut(s) 640
HpyAV CCTTC 4 cut(s) 443, 458, 473, 488
HpyCH4III ACNGT 4 cut(s) 286, 692, 935, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 620
HpyF3I CTNAG 1 cut(s) 990
Hsp92II CATG 2 cut(s) 277, 1034
HspAI GCGC 1 cut(s) 924
Kzo9I GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
LmnI GCTCC 4 cut(s) 212, 219, 246, 667
Lsp1109I GCAGC 3 cut(s) 82, 632, 1034
LweI GCATC 3 cut(s) 511, 607, 632
MaeIII GTNAC 1 cut(s) 41
MalI GATC 7 cut(s) 23, 234, 326, 753, 828, 914, 995
MboI GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
MflI RGATCY 1 cut(s) 912
MhlI GDGCHC 2 cut(s) 642, 672
MluCI AATT 3 cut(s) 301, 563, 1008
MmeI TCCRAC 4 cut(s) 127, 779, 909, 951
MroXI GAANNNNTTC 1 cut(s) 379
MseI TTAA 3 cut(s) 527, 584, 939
MspI CCGG 1 cut(s) 385
MspR9I CCNGG 3 cut(s) 347, 385, 877
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 2 cut(s) 347, 877
MvnI CGCG 1 cut(s) 926
MwoI GCNNNNNNNGC 2 cut(s) 515, 620
NciI CCSGG 1 cut(s) 385
NdeI CATATG 2 cut(s) 7, 766
NdeII GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
NlaIII CATG 2 cut(s) 277, 1034
NlaIV GGNNCC 2 cut(s) 242, 1016
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 266, 958
PfoI TCCNGGA 1 cut(s) 383
PkrI GCNGC 3 cut(s) 97, 622, 1024
PpuMI RGGWCCY 1 cut(s) 684
PscI ACATGT 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 672
Psp5II RGGWCCY 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 345, 875
PspGI CCWGG 2 cut(s) 345, 875
PspN4I GGNNCC 2 cut(s) 242, 1016
PspPI GGNCC 2 cut(s) 684, 983
PspPPI RGGWCCY 1 cut(s) 684
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 1 cut(s) 912
SacI GAGCTC 1 cut(s) 672
SaqAI TTAA 3 cut(s) 527, 584, 939
SatI GCNGC 3 cut(s) 96, 621, 1023
Sau3AI GATC 7 cut(s) 21, 232, 324, 751, 826, 912, 993
Sau96I GGNCC 2 cut(s) 684, 983
ScrFI CCNGG 3 cut(s) 347, 385, 877
SduI GDGCHC 2 cut(s) 642, 672
SetI ASST 9 cut(s) 165, 217, 393, 448, 577, 672, 689, 951, 1027
SfaNI GCATC 3 cut(s) 511, 607, 632
SinI GGWCC 2 cut(s) 684, 983
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 3 cut(s) 301, 563, 1008
SspI AATATT 1 cut(s) 421
SstI GAGCTC 1 cut(s) 672
StyD4I CCNGG 3 cut(s) 345, 383, 875
TaaI ACNGT 4 cut(s) 286, 692, 935, 1019
TaqI TCGA 3 cut(s) 24, 352, 966
TasI AATT 3 cut(s) 301, 563, 1008
TfiI GAWTC 2 cut(s) 266, 958
Tru1I TTAA 3 cut(s) 527, 584, 939
Tru9I TTAA 3 cut(s) 527, 584, 939
TscAI CASTG 1 cut(s) 87
TseI GCWGC 3 cut(s) 95, 620, 1022
TspDTI ATGAA 5 cut(s) 130, 477, 492, 507, 813
TspRI CASTG 1 cut(s) 87
VneI GTGCAC 1 cut(s) 638
VpaK11BI GGWCC 2 cut(s) 684, 983
XceI RCATGY 1 cut(s) 277
XmnI GAANNNNTTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.