Rorug03G0085900

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001475
Physical Location & Seq
Reverse (-)
6638729 .. 6639316
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0085900.1

Sequence Viewer

Length: 483 bp
ATGTATGGTGTTGCTGTAAAATATGGTGTTTCTAAGTTAATTTTTTGCAATTCACAGGCCGATGCAGCATCTGGTATCGCTGTGCACGAAGAGTGCAAGTTGAAGTTTCTGGAGCTCAAGACAAAGAGGACCTACCGTTCAGTAGTTTTCAAGATTGAGGAGGAGCAAAAGCAAGTTATTGTGGAAGCAACTGGTGATCCAACAGAAACATATGAGGCTTTCACAGATAGTCTTCCTACTGATGAATGCTGTTATGCTGTTTTCGATTTCGATCTTCCTATACCAGAGGGTGTCCCAAAAAGGCAGATATTGTTTATTGCCTGGTCGCCTGATACATCAAGGGAGGGAAACAAGATCTATGCAAGCGCGATCGAAAGTTTCAAGAGAGAGCTTGATGGGATTCAAATCGAGTTGGAGGCGACTGGTCCATCTGAGATCAGCCTTGATGTAATTAGGAACCGTGTTGCTAAACATGAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

160

Amino Acids

17.97

Weight (kDa)

4.87

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 25 - 155 8.4e-30 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 368
AclWI GGATC 1 cut(s) 191
AgsI TTSAA 4 cut(s) 103, 151, 382, 404
AjnI CCWGG 1 cut(s) 320
AloI GAACNNNNNNTCC 2 cut(s) 121, 153
AluBI AGCT 2 cut(s) 115, 391
AluI AGCT 2 cut(s) 115, 391
Alw21I GWGCWC 2 cut(s) 87, 117
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 1 cut(s) 57
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 129, 425
AsuHPI GGTGA 1 cut(s) 206
AvaII GGWCC 2 cut(s) 129, 425
BaeGI GKGCMC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 224
Bbv12I GWGCWC 2 cut(s) 87, 117
BbvI GCAGC 1 cut(s) 77
BccI CCATC 2 cut(s) 389, 436
BciT130I CCWGG 1 cut(s) 322
BglII AGATCT 1 cut(s) 354
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 2 cut(s) 129, 425
BmgT120I GGNCC 2 cut(s) 129, 425
BmiI GGNNCC 1 cut(s) 458
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 2 cut(s) 52, 77
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 2 cut(s) 270, 404
Bse1I ACTGG 2 cut(s) 196, 427
Bse8I GATNNNNATC 2 cut(s) 270, 404
BseBI CCWGG 1 cut(s) 322
BseJI GATNNNNATC 2 cut(s) 270, 404
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 2 cut(s) 196, 427
BseRI GAGGAG 2 cut(s) 173, 176
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 368
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 372
BsiHKAI GWGCWC 2 cut(s) 87, 117
BslFI GGGAC 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 87, 117
Bsp143I GATC 5 cut(s) 196, 271, 354, 369, 435
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 1 cut(s) 458
BspPI GGATC 1 cut(s) 191
BsrI ACTGG 2 cut(s) 196, 427
BssMI GATC 5 cut(s) 196, 271, 354, 369, 435
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 137, 461
Bst6I CTCTTC 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 2 cut(s) 33, 432
BstFNI CGCG 1 cut(s) 368
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 5 cut(s) 199, 274, 357, 372, 438
BstMBI GATC 5 cut(s) 196, 271, 354, 369, 435
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 87
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 224
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BsuRI GGCC 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 364
CaiI CAGNNNCTG 1 cut(s) 71
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 2 cut(s) 129, 425
CviAII CATG 1 cut(s) 473
CviJI RGCY 5 cut(s) 59, 115, 218, 391, 441
CviKI_1 RGCY 5 cut(s) 59, 115, 218, 391, 441
DdeI CTNAG 2 cut(s) 33, 432
DpnI GATC 5 cut(s) 198, 273, 356, 371, 437
DpnII GATC 5 cut(s) 196, 271, 354, 369, 435
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 129, 425
Eco53kI GAGCTC 1 cut(s) 115
EcoICRI GAGCTC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 117
FaeI CATG 1 cut(s) 476
FaiI YATR 9 cut(s) 6, 24, 211, 213, 255, 281, 360, 474, 481
FaqI GGGAC 1 cut(s) 278
FatI CATG 1 cut(s) 472
FauNDI CATATG 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 66
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 1 cut(s) 66
GlaI GCGC 1 cut(s) 367
GluI GCNGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 59
HhaI GCGC 1 cut(s) 368
Hin1II CATG 1 cut(s) 476
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HinfI GANTC 1 cut(s) 400
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 4 cut(s) 110, 118, 151, 382
Hpy8I GTNNAC 1 cut(s) 85
HpyCH4III ACNGT 2 cut(s) 137, 461
HpyCH4V TGCA 5 cut(s) 48, 65, 85, 96, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 2 cut(s) 33, 432
Hsp92II CATG 1 cut(s) 476
HspAI GCGC 1 cut(s) 366
Kzo9I GATC 5 cut(s) 196, 271, 354, 369, 435
LmnI GCTCC 2 cut(s) 112, 163
LpnPI CCDG 9 cut(s) 41, 57, 95, 177, 297, 307, 334, 342, 408
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 2 cut(s) 52, 77
MalI GATC 5 cut(s) 198, 273, 356, 371, 437
MboI GATC 5 cut(s) 196, 271, 354, 369, 435
MboII GAAGA 3 cut(s) 101, 224, 266
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 2 cut(s) 87, 117
MluCI AATT 3 cut(s) 39, 49, 450
MmeI TCCRAC 2 cut(s) 224, 393
MnlI CCTC 7 cut(s) 120, 151, 154, 208, 280, 337, 409
MseI TTAA 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 322
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeI CATATG 1 cut(s) 211
NdeII GATC 5 cut(s) 196, 271, 354, 369, 435
NlaIII CATG 1 cut(s) 476
NlaIV GGNNCC 1 cut(s) 458
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 1 cut(s) 67
Ple19I CGATCG 1 cut(s) 372
PpuMI RGGWCCY 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 458
PspPI GGNCC 2 cut(s) 129, 425
PspPPI RGGWCCY 1 cut(s) 129
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 354
PvuI CGATCG 1 cut(s) 372
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 5 cut(s) 196, 271, 354, 369, 435
Sau96I GGNCC 2 cut(s) 129, 425
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 2 cut(s) 87, 117
SetI ASST 3 cut(s) 117, 134, 393
SfaNI GCATC 2 cut(s) 52, 77
SinI GGWCC 2 cut(s) 129, 425
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 3 cut(s) 39, 49, 450
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 2 cut(s) 137, 461
TaqI TCGA 4 cut(s) 264, 270, 372, 408
TasI AATT 3 cut(s) 39, 49, 450
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TseI GCWGC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 258
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 2 cut(s) 129, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.