pycom04g11090

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001475
Physical Location & Seq
Forward (+)
13923891 .. 13925373
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11090.3

Sequence Viewer

Length: 402 bp
ATGGCCAACGCAGCATCTGGCATGGCTGTGCACGATGATTGCAAACTGAAGTTTTTGGAGCTTAAGGCAAAGAGGACCTACCGCTCCATAGTTTTCAAGATTGAGGAGAAGCAAAAGCAGGTTGTGGTTGAACATGTTGGTGAACCAGCGGAAACCTATGAGCAGTTCACTGAGAAGCTTCCTGCTGATGAATGCCGATATGCTATTTTTGATTTTGACTTTCTTACACCAGAAGGTGTCCAGAAAAGTCGGATATTCTTCATTGCTTGGGTGAGAAGCAAGATGATCGACGCAAGTTCCAAAGACAGATTCAAGAGAGAGCTCGATGGCATTCAGGTCGAGTTGCAAGAGATCGATCCTACTGAGATTGGTCTCGATGTCATTAAGAGCCGTGCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

134

Amino Acids

15.34

Weight (kDa)

5.49

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 109
AccBSI CCGCTC 1 cut(s) 84
AciI CCGC 2 cut(s) 82, 149
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 68
AflII CTTAAG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 133
AgsI TTSAA 3 cut(s) 97, 131, 313
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 4 cut(s) 61, 178, 322, 399
AluI AGCT 4 cut(s) 61, 178, 322, 399
Alw21I GWGCWC 2 cut(s) 33, 324
Alw26I GTCTC 1 cut(s) 377
Alw44I GTGCAC 1 cut(s) 29
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 75
AsuHPI GGTGA 2 cut(s) 152, 283
AvaII GGWCC 1 cut(s) 75
BaeGI GKGCMC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 33, 324
BbvI GCAGC 1 cut(s) 23
BccI CCATC 1 cut(s) 320
BceAI ACGGC 1 cut(s) 375
BcgI CGANNNNNNTGC 4 cut(s) 268, 302, 319, 353
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 1 cut(s) 400
BfrI CTTAAG 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmsI GCATC 1 cut(s) 23
Bsa29I ATCGAT 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 377
Bse3DI GCAATG 1 cut(s) 261
BseCI ATCGAT 1 cut(s) 354
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 162, 354
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 354
BsiHKAI GWGCWC 2 cut(s) 33, 324
BsmAI GTCTC 1 cut(s) 377
BsmI GAATGC 2 cut(s) 197, 330
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 33, 324
Bsp143I GATC 3 cut(s) 285, 351, 355
BspACI CCGC 2 cut(s) 82, 149
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 163, 355
BspDI ATCGAT 1 cut(s) 354
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 350
BspTI CTTAAG 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 261
BssMI GATC 3 cut(s) 285, 351, 355
BstAFI CTTAAG 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 2 cut(s) 171, 363
BstKTI GATC 3 cut(s) 288, 354, 358
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 3 cut(s) 285, 351, 355
BstMWI GCNNNNNNNGC 2 cut(s) 11, 396
BstNSI RCATGY 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 354
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 397
CaiI CAGNNNCTG 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 75
ClaI ATCGAT 1 cut(s) 354
CseI GACGC 1 cut(s) 299
CviAII CATG 2 cut(s) 22, 134
CviJI RGCY 7 cut(s) 5, 26, 61, 178, 322, 390, 399
CviKI_1 RGCY 7 cut(s) 5, 26, 61, 178, 322, 390, 399
DdeI CTNAG 2 cut(s) 171, 363
DpnI GATC 3 cut(s) 287, 353, 357
DpnII GATC 3 cut(s) 285, 351, 355
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 68
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 324
FaeI CATG 2 cut(s) 25, 137
FaiI YATR 5 cut(s) 23, 89, 135, 159, 201
FatI CATG 2 cut(s) 21, 133
Fnu4HI GCNGC 1 cut(s) 12
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 400
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 299
Hin1II CATG 2 cut(s) 25, 137
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 1 cut(s) 309
HphI GGTGA 2 cut(s) 152, 283
Hpy166II GTNNAC 3 cut(s) 31, 143, 168
Hpy188I TCNGA 1 cut(s) 252
Hpy188III TCNNGA 4 cut(s) 97, 241, 313, 374
Hpy8I GTNNAC 3 cut(s) 31, 143, 168
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 1 cut(s) 227
HpyCH4V TGCA 3 cut(s) 31, 42, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 396
HpyF3I CTNAG 2 cut(s) 171, 363
Hsp92II CATG 2 cut(s) 25, 137
Kzo9I GATC 3 cut(s) 285, 351, 355
LmnI GCTCC 2 cut(s) 58, 89
LpnPI CCDG 7 cut(s) 3, 104, 159, 195, 243, 254, 320
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 400
MalI GATC 3 cut(s) 287, 353, 357
MbiI CCGCTC 1 cut(s) 84
MboI GATC 3 cut(s) 285, 351, 355
MboII GAAGA 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 33, 324
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 230
MnlI CCTC 2 cut(s) 66, 97
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 63, 384
MslI CAYNNNNRTG 2 cut(s) 26, 138
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 149
MspCI CTTAAG 1 cut(s) 62
Mva1269I GAATGC 2 cut(s) 197, 330
MwoI GCNNNNNNNGC 2 cut(s) 11, 396
NdeII GATC 3 cut(s) 285, 351, 355
NlaIII CATG 2 cut(s) 25, 137
NspI RCATGY 1 cut(s) 137
PciI ACATGT 1 cut(s) 133
PctI GAATGC 2 cut(s) 197, 330
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 75
PscI ACATGT 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 75
PspPI GGNCC 1 cut(s) 75
PspPPI RGGWCCY 1 cut(s) 75
PstNI CAGNNNCTG 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 26, 138
SacI GAGCTC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 63, 384
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 3 cut(s) 285, 351, 355
Sau96I GGNCC 1 cut(s) 75
SduI GDGCHC 2 cut(s) 33, 324
SetI ASST 9 cut(s) 63, 80, 123, 158, 180, 238, 324, 339, 401
SfaNI GCATC 1 cut(s) 23
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 2 cut(s) 26, 138
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SsiI CCGC 2 cut(s) 82, 149
SspMI CTAG 1 cut(s) 400
SstI GAGCTC 1 cut(s) 324
TaqI TCGA 5 cut(s) 288, 324, 339, 354, 375
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 2 cut(s) 63, 384
Tru9I TTAA 2 cut(s) 63, 384
TscAI CASTG 1 cut(s) 175
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 2 cut(s) 204, 250
TspRI CASTG 1 cut(s) 175
Vha464I CTTAAG 1 cut(s) 62
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 75
XceI RCATGY 1 cut(s) 137
XspI CTAG 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.