pycom07g18120

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001475
Physical Location & Seq
Reverse (-)
20614339 .. 20615661
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18120.1

Sequence Viewer

Length: 420 bp
ATGGCAAATGCAGCATCTGGGATGGCTGTGCATGGCGAATGCAAGCTAAAGTTTCTGGAATTGAAGGCGAGAAGAACTTACCGGTTCATAGTTTACAAGATTGAGGAGAAACAAAATGGGGTTGTTGTGGAAAAGCTTGGTGAGCCAACTGACAGCTACGAGGATTTCTCTGCAAGCCTCCCTGCTAACGAGTGTCGATACGCTGTTTATGACTTTGATTACGTGACAGAGGAGAACTGCCAGAAGAGCCGGATTGTTTTCGTTGCTTGGTCCCCTGATACAGCGAAGGTGAGAAGCAAGATGATTTATGCGAGTTCGAAGGACAGGTTTAAGAGAGAGTTGGACGGAATTCAGGTAGAGTTGCAAGCTACTGATCCTACCGAAGTGGGTCTCGATGTTATTAGAAGCCGCGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

140

Amino Acids

15.84

Weight (kDa)

5.92

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 138 5.2e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 411
AciI CCGC 1 cut(s) 409
AclWI GGATC 1 cut(s) 368
AcsI RAATTY 1 cut(s) 348
AgeI ACCGGT 1 cut(s) 81
AgsI TTSAA 1 cut(s) 64
AluBI AGCT 4 cut(s) 46, 136, 156, 368
AluI AGCT 4 cut(s) 46, 136, 156, 368
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 17
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 348
AsiGI ACCGGT 1 cut(s) 81
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 152, 301
AsuII TTCGAA 1 cut(s) 317
AvaII GGWCC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 23
BccI CCATC 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 395
BisI GCNGC 2 cut(s) 12, 409
BlsI GCNGC 2 cut(s) 13, 410
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 272
BmsI GCATC 1 cut(s) 23
BplI GAGNNNNNCTC 2 cut(s) 152, 184
Bpu14I TTCGAA 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 395
BsaWI WCCGGW 1 cut(s) 81
Bse118I RCCGGY 1 cut(s) 81
BseGI GGATG 1 cut(s) 27
BseRI GAGGAG 2 cut(s) 119, 245
BseXI GCAGC 1 cut(s) 23
Bsh1236I CGCG 1 cut(s) 411
BshTI ACCGGT 1 cut(s) 81
BsiSI CCGG 2 cut(s) 82, 250
BslFI GGGAC 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 395
Bsp119I TTCGAA 1 cut(s) 317
Bsp143I GATC 1 cut(s) 373
BspACI CCGC 1 cut(s) 409
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 272
BspPI GGATC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 239
BspT104I TTCGAA 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 81
BssMI GATC 1 cut(s) 373
Bst6I CTCTTC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 317
BstC8I GCNNGC 3 cut(s) 44, 175, 366
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 411
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 3 cut(s) 11, 142, 246
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 1 cut(s) 23
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 3 cut(s) 44, 175, 366
CaiI CAGNNNCTG 1 cut(s) 17
CfoI GCGC 1 cut(s) 413
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 270
CspAI ACCGGT 1 cut(s) 81
CviAII CATG 1 cut(s) 32
CviJI RGCY 9 cut(s) 26, 46, 136, 145, 156, 177, 249, 368, 408
CviKI_1 RGCY 9 cut(s) 26, 46, 136, 145, 156, 177, 249, 368, 408
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 348
FaeI CATG 1 cut(s) 35
FaiI YATR 4 cut(s) 33, 89, 210, 309
FaqI GGGAC 1 cut(s) 256
FatI CATG 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 12, 409
FokI GGATG 1 cut(s) 34
Fsp4HI GCNGC 2 cut(s) 12, 409
GlaI GCGC 1 cut(s) 412
GluI GCNGC 2 cut(s) 12, 409
HapII CCGG 2 cut(s) 82, 250
HhaI GCGC 1 cut(s) 413
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 134
HpaII CCGG 2 cut(s) 82, 250
HphI GGTGA 2 cut(s) 152, 301
Hpy166II GTNNAC 1 cut(s) 94
Hpy188III TCNNGA 2 cut(s) 56, 392
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 3 cut(s) 58, 280, 313
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 11, 31, 42, 173, 364
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 142, 246
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 1 cut(s) 411
Kzo9I GATC 1 cut(s) 373
LguI GCTCTTC 1 cut(s) 239
LpnPI CCDG 9 cut(s) 3, 41, 95, 195, 254, 263, 288, 310, 338
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 23
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 223
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 2 cut(s) 84, 256
MluCI AATT 3 cut(s) 59, 348, 415
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 4 cut(s) 97, 154, 188, 223
MseI TTAA 1 cut(s) 330
MspI CCGG 2 cut(s) 82, 250
Mva1269I GAATGC 1 cut(s) 44
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 3 cut(s) 11, 142, 246
NdeII GATC 1 cut(s) 373
NlaIII CATG 1 cut(s) 35
NlaIV GGNNCC 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 223
NspV TTCGAA 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 239
PctI GAATGC 1 cut(s) 44
PinAI ACCGGT 1 cut(s) 81
PkrI GCNGC 2 cut(s) 13, 410
Ppu21I YACGTR 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 270
PstNI CAGNNNCTG 1 cut(s) 17
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 2 cut(s) 12, 409
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 1 cut(s) 270
SetI ASST 8 cut(s) 48, 138, 158, 225, 291, 329, 357, 370
SfaNI GCATC 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 317
SinI GGWCC 1 cut(s) 270
Sse9I AATT 3 cut(s) 59, 348, 415
SsiI CCGC 1 cut(s) 409
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 196, 317, 393
TasI AATT 3 cut(s) 59, 348, 415
TauI GCSGC 1 cut(s) 411
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 76
TspGWI ACGGA 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 270
XapI RAATTY 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.