Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001475

Physical Location & Seq

Genomic Coordinates Forward (+)

20822303 .. 20824458

Gene Structure 2156 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1122600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 420 bp

ATGGCCAACGCAGCATCTGGTATGGCTGTGCACGATGATTGCAAACTGAAGTTTTTGGAGCTTAAGGCAAAGAGGACCTACCGCTCCATAGTTTTCAAGATTGAGGAGAAGCAAAAGCAGGTTGTGGTGGAACATGTTGGTGAACCAGCAGAAACCTATGAGCAGTTCACTGAGAAGCTTCCTGCTGATGAATGCCGATATGCTATTTTTGATTTTGACTTCCTTACACCAGAAGGTGTCCAGAAAAGTCGGATATTCTTCATTGCCTGGTCTCCTGATACATCAAGGGTGAGAAGCAAGATGATCTACGCAAGCTCCAAAGACAGATTCAAGAGAGAGCTCGATGGCATTCAGGTTGAGTTGCAAGCGACTGATCCTACTGAGATTGGTCTTGATGTCATTAAGAGCCGTGCCAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133

Pfam Domains

Protein Families

Cofilin_ADF

Protein Analysis

140

Amino Acids

15.96

Weight (kDa)

6.16

Isoelectric Point (pI)

49.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cofilin_ADF	PF00241	7 - 138	4.7e-39	Cofilin/tropomyosin-type actin-binding protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46000 AT3G46000 AT3G46000 AT3G46010 AT3G46010 AT5G59880 AT5G59890 AT5G59890 AT5G59890
fragaria_vesca	FvH4_6g14260 FvH4_6g14260 FvH4_6g14260 FvH4_6g14260 FvH4_7g21550
malus_domestica	MD01G1122300.v1.1 MD04G1122600.v1.1 MD07G1191800.v1.1 MD12G1138800.v1.1
prunus_persica	Prupe.2G229900_v2.0.a1 Prupe.6G250700_v2.0.a1 Prupe.6G250700_v2.0.a1 Prupe.6G250700_v2.0.a1
pyrus_communis	pycom04g11090 pycom07g18120 pycom12g13310
rosa_chinensis	RchiOBHm_Chr1g0365871 RchiOBHm_Chr3g0467221
rosa_laevigata	RLG00000024518 RLG00000027384
rosa_multiflora	Rmu_co8241371.1_g000001 Rmu_sc0004288.1_g000018 Rmu_sc0004404.1_g000004 Rmu_sc0004404.1_g000011 Rmu_sc0006406.1_g000003
rosa_roxburghii	Rroxscaffold_6G00413810 Rroxscaffold_6G00420950
rosa_rugosa	Rorug01G0325000 Rorug03G0030100.1 Rorug03G0085800 Rorug03G0085900
rosa_samantha	Rh1AG334200 Rh1BG294800 Rh1CG311500 Rh1DG327200 Rh3AG134300 Rh3BG155600 Rh3CG091500 Rh3CG155200 Rh3DG156400
rosa_wichuraiana	Rw1G029650 Rw3G012710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	109
AccBSI	CCGCTC	1 cut(s)	84
AciI	CCGC	1 cut(s)	82
AclWI	GGATC	1 cut(s)	368
AcoI	YGGCCR	1 cut(s)	3
AcuI	CTGAAG	1 cut(s)	68
AflII	CTTAAG	1 cut(s)	62
AflIII	ACRYGT	1 cut(s)	133
AgsI	TTSAA	2 cut(s)	97, 331
AjnI	CCWGG	1 cut(s)	266
AjuI	GAANNNNNNNTTGG	2 cut(s)	311, 343
AluBI	AGCT	5 cut(s)	61, 178, 315, 340, 417
AluI	AGCT	5 cut(s)	61, 178, 315, 340, 417
Alw21I	GWGCWC	2 cut(s)	33, 342
Alw26I	GTCTC	1 cut(s)	276
Alw44I	GTGCAC	1 cut(s)	29
AlwI	GGATC	1 cut(s)	368
AlwNI	CAGNNNCTG	1 cut(s)	17
AoxI	GGCC	1 cut(s)	3
ApaLI	GTGCAC	1 cut(s)	29
ApeKI	GCWGC	1 cut(s)	11
AspS9I	GGNCC	1 cut(s)	75
AsuHPI	GGTGA	2 cut(s)	152, 301
AvaII	GGWCC	1 cut(s)	75
BaeGI	GKGCMC	1 cut(s)	33
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	342
Bbv12I	GWGCWC	2 cut(s)	33, 342
BbvI	GCAGC	1 cut(s)	23
BccI	CCATC	1 cut(s)	338
BceAI	ACGGC	1 cut(s)	393
BciT130I	CCWGG	1 cut(s)	268
BcoDI	GTCTC	1 cut(s)	276
BfaI	CTAG	1 cut(s)	418
BfrI	CTTAAG	1 cut(s)	62
BfuAI	ACCTGC	1 cut(s)	109
BisI	GCNGC	1 cut(s)	12
BlsI	GCNGC	1 cut(s)	13
Bme1390I	CCNGG	1 cut(s)	268
Bme18I	GGWCC	1 cut(s)	75
BmgT120I	GGNCC	1 cut(s)	75
BmrFI	CCNGG	1 cut(s)	268
BmsI	GCATC	1 cut(s)	23
BsaI	GGTCTC	1 cut(s)	276
BsaXI	ACNNNNNCTCC	2 cut(s)	299, 329
Bse3DI	GCAATG	1 cut(s)	261
BseBI	CCWGG	1 cut(s)	268
BseMI	GCAATG	1 cut(s)	261
BseMII	CTCAG	2 cut(s)	162, 372
BseRI	GAGGAG	1 cut(s)	119
BseSI	GKGCMC	1 cut(s)	33
BseXI	GCAGC	1 cut(s)	23
BshFI	GGCC	1 cut(s)	5
BsiHKAI	GWGCWC	2 cut(s)	33, 342
BsmAI	GTCTC	1 cut(s)	276
BsmI	GAATGC	2 cut(s)	197, 348
BsnI	GGCC	1 cut(s)	5
Bso31I	GGTCTC	1 cut(s)	276
Bsp1286I	GDGCHC	2 cut(s)	33, 342
Bsp143I	GATC	2 cut(s)	303, 373
BspACI	CCGC	1 cut(s)	82
BspANI	GGCC	1 cut(s)	5
BspCNI	CTCAG	2 cut(s)	163, 373
BspMI	ACCTGC	1 cut(s)	109
BspPI	GGATC	1 cut(s)	368
BspTI	CTTAAG	1 cut(s)	62
BspTNI	GGTCTC	1 cut(s)	276
BsrBI	CCGCTC	1 cut(s)	84
BsrDI	GCAATG	1 cut(s)	261
BssMI	GATC	2 cut(s)	303, 373
Bst2UI	CCWGG	1 cut(s)	268
BstAFI	CTTAAG	1 cut(s)	62
BstC8I	GCNNGC	3 cut(s)	313, 366, 415
BstDEI	CTNAG	2 cut(s)	171, 381
BstKTI	GATC	2 cut(s)	306, 376
BstMAI	GTCTC	1 cut(s)	276
BstMBI	GATC	2 cut(s)	303, 373
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 414
BstNI	CCWGG	1 cut(s)	268
BstNSI	RCATGY	1 cut(s)	137
BstSCI	CCNGG	1 cut(s)	266
BstSLI	GKGCMC	1 cut(s)	33
BstV1I	GCAGC	1 cut(s)	23
BsuRI	GGCC	1 cut(s)	5
BtsIMutI	CAGTG	1 cut(s)	168
BveI	ACCTGC	1 cut(s)	109
Cac8I	GCNNGC	3 cut(s)	313, 366, 415
CaiI	CAGNNNCTG	1 cut(s)	17
Cfr13I	GGNCC	1 cut(s)	75
CviAII	CATG	1 cut(s)	134
CviJI	RGCY	8 cut(s)	5, 26, 61, 178, 315, 340, 408, 417
CviKI_1	RGCY	8 cut(s)	5, 26, 61, 178, 315, 340, 408, 417
DdeI	CTNAG	2 cut(s)	171, 381
DpnI	GATC	2 cut(s)	305, 375
DpnII	GATC	2 cut(s)	303, 373
EaeI	YGGCCR	1 cut(s)	3
Ecl136II	GAGCTC	1 cut(s)	340
Eco24I	GRGCYC	1 cut(s)	342
Eco31I	GGTCTC	1 cut(s)	276
Eco47I	GGWCC	1 cut(s)	75
Eco53kI	GAGCTC	1 cut(s)	340
Eco57I	CTGAAG	1 cut(s)	68
EcoICRI	GAGCTC	1 cut(s)	340
EcoO109I	RGGNCCY	1 cut(s)	75
EcoRII	CCWGG	1 cut(s)	266
EcoT38I	GRGCYC	1 cut(s)	342
FaeI	CATG	1 cut(s)	137
FaiI	YATR	5 cut(s)	23, 89, 135, 159, 201
FatI	CATG	1 cut(s)	133
Fnu4HI	GCNGC	1 cut(s)	12
FriOI	GRGCYC	1 cut(s)	342
Fsp4HI	GCNGC	1 cut(s)	12
FspBI	CTAG	1 cut(s)	418
GluI	GCNGC	1 cut(s)	12
HaeIII	GGCC	1 cut(s)	5
Hin1II	CATG	1 cut(s)	137
HindIII	AAGCTT	1 cut(s)	176
HinfI	GANTC	1 cut(s)	327
HphI	GGTGA	2 cut(s)	152, 301
Hpy166II	GTNNAC	3 cut(s)	31, 143, 168
Hpy188I	TCNGA	1 cut(s)	252
Hpy188III	TCNNGA	5 cut(s)	97, 241, 275, 331, 392
Hpy8I	GTNNAC	3 cut(s)	31, 143, 168
HpyAV	CCTTC	1 cut(s)	227
HpyCH4V	TGCA	3 cut(s)	31, 42, 364
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 414
HpyF3I	CTNAG	2 cut(s)	171, 381
Hsp92II	CATG	1 cut(s)	137
Kzo9I	GATC	2 cut(s)	303, 373
LmnI	GCTCC	3 cut(s)	58, 89, 320
Lsp1109I	GCAGC	1 cut(s)	23
LweI	GCATC	1 cut(s)	23
MaeI	CTAG	1 cut(s)	418
MalI	GATC	2 cut(s)	305, 375
MbiI	CCGCTC	1 cut(s)	84
MboI	GATC	2 cut(s)	303, 373
MboII	GAAGA	1 cut(s)	250
MhlI	GDGCHC	2 cut(s)	33, 342
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MmeI	TCCRAC	1 cut(s)	230
MnlI	CCTC	2 cut(s)	66, 97
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	63, 402
MslI	CAYNNNNRTG	1 cut(s)	138
Msp20I	TGGCCA	1 cut(s)	5
MspCI	CTTAAG	1 cut(s)	62
MspR9I	CCNGG	1 cut(s)	268
Mva1269I	GAATGC	2 cut(s)	197, 348
MvaI	CCWGG	1 cut(s)	268
MwoI	GCNNNNNNNGC	2 cut(s)	11, 414
NdeII	GATC	2 cut(s)	303, 373
NlaIII	CATG	1 cut(s)	137
NspI	RCATGY	1 cut(s)	137
PciI	ACATGT	1 cut(s)	133
PctI	GAATGC	2 cut(s)	197, 348
PfeI	GAWTC	1 cut(s)	327
PkrI	GCNGC	1 cut(s)	13
PpuMI	RGGWCCY	1 cut(s)	75
PscI	ACATGT	1 cut(s)	133
Psp124BI	GAGCTC	1 cut(s)	342
Psp5II	RGGWCCY	1 cut(s)	75
Psp6I	CCWGG	1 cut(s)	266
PspGI	CCWGG	1 cut(s)	266
PspPI	GGNCC	1 cut(s)	75
PspPPI	RGGWCCY	1 cut(s)	75
PstNI	CAGNNNCTG	1 cut(s)	17
RseI	CAYNNNNRTG	1 cut(s)	138
SacI	GAGCTC	1 cut(s)	342
SaqAI	TTAA	2 cut(s)	63, 402
SatI	GCNGC	1 cut(s)	12
Sau3AI	GATC	2 cut(s)	303, 373
Sau96I	GGNCC	1 cut(s)	75
ScrFI	CCNGG	1 cut(s)	268
SduI	GDGCHC	2 cut(s)	33, 342
SfaNI	GCATC	1 cut(s)	23
SinI	GGWCC	1 cut(s)	75
SmiMI	CAYNNNNRTG	1 cut(s)	138
SmlI	CTYRAG	1 cut(s)	62
SmoI	CTYRAG	1 cut(s)	62
SsiI	CCGC	1 cut(s)	82
SspMI	CTAG	1 cut(s)	418
SstI	GAGCTC	1 cut(s)	342
StyD4I	CCNGG	1 cut(s)	266
TaqI	TCGA	1 cut(s)	342
TfiI	GAWTC	1 cut(s)	327
Tru1I	TTAA	2 cut(s)	63, 402
Tru9I	TTAA	2 cut(s)	63, 402
TscAI	CASTG	1 cut(s)	175
TseI	GCWGC	1 cut(s)	11
TspDTI	ATGAA	2 cut(s)	204, 250
TspRI	CASTG	1 cut(s)	175
Vha464I	CTTAAG	1 cut(s)	62
VneI	GTGCAC	1 cut(s)	29
VpaK11BI	GGWCC	1 cut(s)	75
XceI	RCATGY	1 cut(s)	137
XspI	CTAG	1 cut(s)	418

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.