pycom12g13310

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001475
Physical Location & Seq
Forward (+)
15789712 .. 15791894
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13310.1

Sequence Viewer

Length: 753 bp
ATGACGGTTCATCATTATAGAGACCGAAAGACTCACAGAGAAATCAGTGCATTTCTCATAAGCCGAGTCTGGAGAAGCCGACTCAAGCAAAGCCACTCCCGCATCCATACACAAAGACCCGTACAAGTCGTGGCGGCACGCCATTGGCCGAACCGCCCATCACCCGCTCCTCTATCATACGATCAGCTCCACGTTGTCAATCCTCTGGCATCCAGAGAGCCACCAAATATAAAGGGCCCTTGGACATTTCTTGTGCGAGACACAGATAGCCGAGTCGTCATCGGAGGAGACCCTTTCCCTATATCTTCTCCTCCTCCTTCGTCGCCAACAGAGAACCGAACAGCAGCGAAAATGGCCAACGCAGCATCTGGTATGGCTGTGCACGATGATTGCAAACTGAAGTTTTTGGAGCTTAAGGCAAAGCGGACCTACCGCTCCATAGTTTTCAAGATTGAGGAGAAGCAAAAGCAAGTTGTGGTGGAACATGTTGGTGAACCAGCAGAAACCTATGAGCAGTTCACTGAGAAGCTTCCTGCTGATGAATGCCGTTATGCTATTTTTGATTTTGACTTTCTTACACCTGAAGGTGTCCAGAAAAGCCGGATATTCTTCATTGCCTGGGTGAGAAGCAAGATGATCTACCCAAGCTCCAAAGACAGATTCAAGAGAGAGCTCGATGGAATTCAGGTTGAGTTGCAAGCGATCGATCCAACTGAGATTGGTCTCGATGTCATTAAGAGCCGAGCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

251

Amino Acids

28.71

Weight (kDa)

9.58

Isoelectric Point (pI)

61.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 124 - 249 9.7e-29 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 167, 435
AciI CCGC 6 cut(s) 100, 134, 154, 165, 424, 433
AclWI GGATC 1 cut(s) 701
AcoI YGGCCR 2 cut(s) 146, 354
AcsI RAATTY 1 cut(s) 681
AcuI CTGAAG 2 cut(s) 419, 603
AfaI GTAC 1 cut(s) 123
AflII CTTAAG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 484
AgsI TTSAA 2 cut(s) 448, 664
AjnI CCWGG 1 cut(s) 617
AjuI GAANNNNNNNTTGG 2 cut(s) 644, 676
AluBI AGCT 6 cut(s) 187, 412, 529, 648, 673, 750
AluI AGCT 6 cut(s) 187, 412, 529, 648, 673, 750
Alw21I GWGCWC 2 cut(s) 384, 675
Alw26I GTCTC 4 cut(s) 15, 252, 282, 728
Alw44I GTGCAC 1 cut(s) 380
AlwI GGATC 1 cut(s) 701
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 3 cut(s) 146, 235, 354
ApaI GGGCCC 1 cut(s) 239
ApaLI GTGCAC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 344, 362
ApoI RAATTY 1 cut(s) 681
AspS9I GGNCC 3 cut(s) 235, 236, 426
AsuHPI GGTGA 3 cut(s) 153, 503, 634
AvaII GGWCC 1 cut(s) 426
BaeGI GKGCMC 2 cut(s) 239, 384
BalI TGGCCA 1 cut(s) 356
BanII GRGCYC 2 cut(s) 239, 675
Bbv12I GWGCWC 2 cut(s) 384, 675
BbvI GCAGC 2 cut(s) 356, 374
BccI CCATC 2 cut(s) 166, 671
BceAI ACGGC 1 cut(s) 531
BciT130I CCWGG 1 cut(s) 619
BcoDI GTCTC 4 cut(s) 15, 252, 282, 728
BfaI CTAG 1 cut(s) 751
BfrI CTTAAG 1 cut(s) 413
BisI GCNGC 3 cut(s) 135, 345, 363
BlsI GCNGC 3 cut(s) 136, 346, 364
Bme1390I CCNGG 1 cut(s) 619
Bme18I GGWCC 1 cut(s) 426
BmgT120I GGNCC 3 cut(s) 235, 236, 426
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 3 cut(s) 111, 218, 374
BpmI CTGGAG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 705
BsaI GGTCTC 3 cut(s) 15, 282, 728
BsaJI CCNNGG 2 cut(s) 239, 618
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bse3DI GCAATG 1 cut(s) 612
BseBI CCWGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 2 cut(s) 239, 618
BseGI GGATG 2 cut(s) 102, 209
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 2 cut(s) 513, 705
BseRI GAGGAG 5 cut(s) 159, 300, 300, 303, 470
BseSI GKGCMC 2 cut(s) 239, 384
BseXI GCAGC 2 cut(s) 356, 374
Bsh1285I CGRYCG 1 cut(s) 705
BshFI GGCC 3 cut(s) 148, 237, 356
BshVI ATCGAT 1 cut(s) 705
BsiEI CGRYCG 1 cut(s) 705
BsiHKAI GWGCWC 2 cut(s) 384, 675
BsiSI CCGG 1 cut(s) 601
BsmAI GTCTC 4 cut(s) 15, 252, 282, 728
BsmI GAATGC 1 cut(s) 548
BsnI GGCC 3 cut(s) 148, 237, 356
Bso31I GGTCTC 3 cut(s) 15, 282, 728
Bsp120I GGGCCC 1 cut(s) 235
Bsp1286I GDGCHC 3 cut(s) 239, 384, 675
Bsp143I GATC 4 cut(s) 181, 636, 702, 706
BspACI CCGC 6 cut(s) 100, 134, 154, 165, 424, 433
BspANI GGCC 3 cut(s) 148, 237, 356
BspCNI CTCAG 2 cut(s) 514, 706
BspDI ATCGAT 1 cut(s) 705
BspLI GGNNCC 1 cut(s) 237
BspPI GGATC 1 cut(s) 701
BspTI CTTAAG 1 cut(s) 413
BspTNI GGTCTC 3 cut(s) 15, 282, 728
BsrBI CCGCTC 2 cut(s) 167, 435
BsrDI GCAATG 1 cut(s) 612
BssECI CCNNGG 2 cut(s) 239, 618
BssMI GATC 4 cut(s) 181, 636, 702, 706
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 1 cut(s) 7
BstAFI CTTAAG 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 139, 699, 748
BstDEI CTNAG 2 cut(s) 522, 714
BstF5I GGATG 2 cut(s) 102, 209
BstKTI GATC 4 cut(s) 184, 639, 705, 709
BstMAI GTCTC 4 cut(s) 15, 252, 282, 728
BstMBI GATC 4 cut(s) 181, 636, 702, 706
BstMCI CGRYCG 1 cut(s) 705
BstMWI GCNNNNNNNGC 4 cut(s) 99, 353, 362, 747
BstNI CCWGG 1 cut(s) 619
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 617
BstSLI GKGCMC 2 cut(s) 239, 384
BstV1I GCAGC 2 cut(s) 356, 374
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 3 cut(s) 148, 237, 356
BsuTUI ATCGAT 1 cut(s) 705
BtsCI GGATG 2 cut(s) 102, 209
BtsIMutI CAGTG 2 cut(s) 52, 519
Cac8I GCNNGC 3 cut(s) 139, 699, 748
CaiI CAGNNNCTG 1 cut(s) 368
Cfr13I GGNCC 3 cut(s) 235, 236, 426
ClaI ATCGAT 1 cut(s) 705
Csp6I GTAC 1 cut(s) 122
CviAII CATG 1 cut(s) 485
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 2 cut(s) 522, 714
DpnI GATC 4 cut(s) 183, 638, 704, 708
DpnII GATC 4 cut(s) 181, 636, 702, 706
EaeI YGGCCR 2 cut(s) 146, 354
Ecl136II GAGCTC 1 cut(s) 673
Eco130I CCWWGG 1 cut(s) 239
Eco24I GRGCYC 2 cut(s) 239, 675
Eco31I GGTCTC 3 cut(s) 15, 282, 728
Eco47I GGWCC 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 2 cut(s) 419, 603
EcoICRI GAGCTC 1 cut(s) 673
EcoO109I RGGNCCY 2 cut(s) 235, 236
EcoRI GAATTC 1 cut(s) 681
EcoRII CCWGG 1 cut(s) 617
EcoT14I CCWWGG 1 cut(s) 239
EcoT38I GRGCYC 2 cut(s) 239, 675
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 1 cut(s) 488
FatI CATG 1 cut(s) 484
FauI CCCGC 2 cut(s) 107, 172
Fnu4HI GCNGC 3 cut(s) 135, 345, 363
FokI GGATG 2 cut(s) 89, 196
FriOI GRGCYC 2 cut(s) 239, 675
Fsp4HI GCNGC 3 cut(s) 135, 345, 363
FspBI CTAG 1 cut(s) 751
GluI GCNGC 3 cut(s) 135, 345, 363
GsuI CTGGAG 1 cut(s) 91
HaeIII GGCC 3 cut(s) 148, 237, 356
HapII CCGG 1 cut(s) 601
Hin1II CATG 1 cut(s) 488
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 5 cut(s) 31, 66, 81, 273, 660
HpaII CCGG 1 cut(s) 601
HphI GGTGA 3 cut(s) 153, 503, 634
Hpy166II GTNNAC 3 cut(s) 382, 494, 519
Hpy188I TCNGA 1 cut(s) 284
Hpy188III TCNNGA 6 cut(s) 70, 213, 448, 592, 664, 725
Hpy8I GTNNAC 3 cut(s) 382, 494, 519
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 327, 578
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 4 cut(s) 50, 382, 393, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 353, 362, 747
HpyF3I CTNAG 2 cut(s) 522, 714
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 1 cut(s) 488
Kzo9I GATC 4 cut(s) 181, 636, 702, 706
LmnI GCTCC 5 cut(s) 172, 192, 409, 440, 653
Lsp1109I GCAGC 2 cut(s) 356, 374
LweI GCATC 3 cut(s) 111, 218, 374
MaeI CTAG 1 cut(s) 751
MaeII ACGT 1 cut(s) 192
MalI GATC 4 cut(s) 183, 638, 704, 708
MbiI CCGCTC 2 cut(s) 167, 435
MboI GATC 4 cut(s) 181, 636, 702, 706
MboII GAAGA 2 cut(s) 297, 601
MhlI GDGCHC 3 cut(s) 239, 384, 675
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 1 cut(s) 681
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 4 cut(s) 25, 75, 75, 282
MmeI TCCRAC 1 cut(s) 734
MnlI CCTC 6 cut(s) 180, 213, 278, 321, 324, 448
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
MseI TTAA 2 cut(s) 414, 735
MslI CAYNNNNRTG 1 cut(s) 489
Msp20I TGGCCA 1 cut(s) 356
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 1 cut(s) 601
MspR9I CCNGG 1 cut(s) 619
Mva1269I GAATGC 1 cut(s) 548
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 4 cut(s) 99, 353, 362, 747
NdeII GATC 4 cut(s) 181, 636, 702, 706
NlaIII CATG 1 cut(s) 488
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 2 cut(s) 89, 296
NspI RCATGY 1 cut(s) 488
PciI ACATGT 1 cut(s) 484
PctI GAATGC 1 cut(s) 548
PfeI GAWTC 1 cut(s) 660
PkrI GCNGC 3 cut(s) 136, 346, 364
Ple19I CGATCG 1 cut(s) 705
PleI GAGTC 4 cut(s) 25, 74, 75, 281
PpsI GAGTC 4 cut(s) 25, 74, 75, 281
PscI ACATGT 1 cut(s) 484
Psp124BI GAGCTC 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 617
PspGI CCWGG 1 cut(s) 617
PspN4I GGNNCC 1 cut(s) 237
PspOMI GGGCCC 1 cut(s) 235
PspPI GGNCC 3 cut(s) 235, 236, 426
PstNI CAGNNNCTG 1 cut(s) 368
PvuI CGATCG 1 cut(s) 705
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
RseI CAYNNNNRTG 1 cut(s) 489
SacI GAGCTC 1 cut(s) 675
SaqAI TTAA 2 cut(s) 414, 735
SatI GCNGC 3 cut(s) 135, 345, 363
Sau3AI GATC 4 cut(s) 181, 636, 702, 706
Sau96I GGNCC 3 cut(s) 235, 236, 426
SchI GAGTC 4 cut(s) 25, 75, 75, 282
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 3 cut(s) 239, 384, 675
SfaNI GCATC 3 cut(s) 111, 218, 374
SinI GGWCC 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 83, 413
SmoI CTYRAG 2 cut(s) 83, 413
Sse9I AATT 1 cut(s) 681
SsiI CCGC 6 cut(s) 100, 134, 154, 165, 424, 433
SspMI CTAG 1 cut(s) 751
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 1 cut(s) 7
TaiI ACGT 1 cut(s) 195
TaqI TCGA 3 cut(s) 675, 705, 726
TaqII GACCGA 1 cut(s) 39
TasI AATT 1 cut(s) 681
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 660
Tru1I TTAA 2 cut(s) 414, 735
Tru9I TTAA 2 cut(s) 414, 735
TscAI CASTG 2 cut(s) 52, 526
TseI GCWGC 2 cut(s) 344, 362
TspDTI ATGAA 2 cut(s) 555, 601
TspRI CASTG 2 cut(s) 52, 526
Vha464I CTTAAG 1 cut(s) 413
VneI GTGCAC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 426
XapI RAATTY 1 cut(s) 681
XceI RCATGY 1 cut(s) 488
XspI CTAG 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.