Rmu_sc0004404.1_g000011

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004404.1
OG0001475
Physical Location & Seq
Reverse (-)
41783 .. 45133
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004404.1_g000011.1.cds

Sequence Viewer

Length: 1239 bp
atggccttgattagcaattgcatcggcaacaaagttggcatatcgaagaatgtggctgaatcagatgttgtcaaaattggccgcaagattgttgatatctcttaccaagggcgttactggacaactgaagacgataagaaactgaaatatgcagtgaagaaagttgctgctcgtaagggaggacacatcaaagattgggagaaagtgggtttcatagtcggaaaaaggggagaaaggcgttactggacaactgaagacgataagaaactgaaatatgcagtgaagaaagttgctgctcgtaagggaggacacatcaaagattggaagaaagtggctttcatagtcggaaaaaggggagacctctgcaaagacagatggcagaatgtggtttctccagatataaagaaaggagcttggagcaaggaggaggatcaaaggctcctcaatgcctctgcttcttgtggttcaaacatgttgaatactatgcttgatgtcttcaattcttcttctgtggatggaaagatcagaacactccatcaatgccaggctcgactacaacaacttttgaatggaaaccattcccggaagcttgaagaaaagtcggtagaacaggggaatattgcaggattatatcagcagaaagctgaaggagtgggtgatgaaggagtggctgatgaaggggtggttgatgaaggagtggcttatgcacaagttgctgatgctttaaccctattgcaaaataactataatggagaagaagaattggagataggtgatggcttaaccaatcttttcaaaacttatgcagaggccgatgcagcatctggtattgctgtgcacaatgagtgcaagttgaagtttctggagctcaagacaaagaggacctaccgttcaatagtcttcaagattgaggagaagcaaaagcaagttattgtggaagcaactggtgatccaacagaaacatatgaggctttcacagatagtctgcctactgatgaatgctgttatgctgtttttgattttgatcttcctataccagagggtgtcccaaaaaggcagatattgtttattgcctggttgcctgatacatcaagggaggaaaacaagaagatctacgcaagcgcgttggacagttttaagagagagcttgatgggattcaaatcgagttggaggcaactggtccatctgagatcggccttgatgtaattaggaaccgtgcagctaaacatgaaccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

412

Amino Acids

46.07

Weight (kDa)

7.01

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1124
AciI CCGC 1 cut(s) 82
AclWI GGATC 2 cut(s) 438, 944
AcoI YGGCCR 1 cut(s) 79
AcuI CTGAAG 3 cut(s) 147, 273, 666
AflIII ACRYGT 1 cut(s) 471
AjnI CCWGG 2 cut(s) 543, 1073
AloI GAACNNNNNNTCC 2 cut(s) 874, 906
AluBI AGCT 6 cut(s) 413, 589, 644, 868, 1147, 1223
AluI AGCT 6 cut(s) 413, 589, 644, 868, 1147, 1223
Alw21I GWGCWC 2 cut(s) 840, 870
Alw26I GTCTC 1 cut(s) 351
Alw44I GTGCAC 1 cut(s) 836
AlwI GGATC 2 cut(s) 438, 944
AlwNI CAGNNNCTG 1 cut(s) 824
AoxI GGCC 4 cut(s) 3, 79, 810, 1195
ApaLI GTGCAC 1 cut(s) 836
ApeKI GCWGC 4 cut(s) 167, 293, 818, 1220
Asp700I GAANNNNTTC 1 cut(s) 577
AspLEI GCGC 1 cut(s) 1124
AspS9I GGNCC 2 cut(s) 882, 1181
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 3 cut(s) 668, 785, 959
AvaII GGWCC 2 cut(s) 882, 1181
BaeGI GKGCMC 1 cut(s) 840
BanII GRGCYC 1 cut(s) 870
BbsI GAAGAC 4 cut(s) 135, 261, 487, 892
Bbv12I GWGCWC 2 cut(s) 840, 870
BbvI GCAGC 4 cut(s) 154, 280, 830, 1232
BccI CCATC 6 cut(s) 369, 509, 543, 770, 1145, 1192
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 2 cut(s) 545, 1075
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 1 cut(s) 351
BglII AGATCT 1 cut(s) 1110
BisI GCNGC 5 cut(s) 82, 168, 294, 819, 1221
BlsI GCNGC 5 cut(s) 83, 169, 295, 820, 1222
Bme1390I CCNGG 3 cut(s) 545, 583, 1075
Bme18I GGWCC 2 cut(s) 882, 1181
BmgT120I GGNCC 2 cut(s) 882, 1181
BmiI GGNNCC 2 cut(s) 440, 1214
BmrFI CCNGG 3 cut(s) 545, 583, 1075
BmsI GCATC 4 cut(s) 30, 709, 805, 830
BpiI GAAGAC 4 cut(s) 135, 261, 487, 892
BpmI CTGGAG 2 cut(s) 378, 884
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 1 cut(s) 583
BsaBI GATNNNNATC 2 cut(s) 1023, 1160
BsaI GGTCTC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 106
Bse1I ACTGG 4 cut(s) 122, 248, 949, 1183
Bse8I GATNNNNATC 2 cut(s) 1023, 1160
BseBI CCWGG 2 cut(s) 545, 1075
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 520
BseJI GATNNNNATC 2 cut(s) 1023, 1160
BseMII CTCAG 1 cut(s) 1179
BseNI ACTGG 4 cut(s) 122, 248, 949, 1183
BseRI GAGGAG 3 cut(s) 431, 440, 926
BseSI GKGCMC 1 cut(s) 840
BseXI GCAGC 4 cut(s) 154, 280, 830, 1232
BsgI GTGCAG 1 cut(s) 1239
Bsh1236I CGCG 1 cut(s) 1124
BshFI GGCC 4 cut(s) 5, 81, 812, 1197
BsiHKAI GWGCWC 2 cut(s) 840, 870
BsiSI CCGG 1 cut(s) 583
BslFI GGGAC 1 cut(s) 1031
BsmAI GTCTC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 1031
BsmI GAATGC 1 cut(s) 1004
BsnI GGCC 4 cut(s) 5, 81, 812, 1197
Bso31I GGTCTC 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 840, 870
Bsp143I GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
BspACI CCGC 1 cut(s) 82
BspANI GGCC 4 cut(s) 5, 81, 812, 1197
BspCNI CTCAG 1 cut(s) 1180
BspFNI CGCG 1 cut(s) 1124
BspLI GGNNCC 2 cut(s) 440, 1214
BspPI GGATC 2 cut(s) 438, 944
BspTNI GGTCTC 1 cut(s) 351
BsrI ACTGG 4 cut(s) 122, 248, 949, 1183
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 2 cut(s) 545, 1075
Bst4CI ACNGT 3 cut(s) 890, 1133, 1217
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 1 cut(s) 1120
BstDEI CTNAG 1 cut(s) 1188
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 1 cut(s) 1124
BstHHI GCGC 1 cut(s) 1124
BstKTI GATC 6 cut(s) 433, 525, 952, 1027, 1113, 1194
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
BstMWI GCNNNNNNNGC 2 cut(s) 713, 818
BstNI CCWGG 2 cut(s) 545, 1075
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 3 cut(s) 543, 581, 1073
BstSLI GKGCMC 1 cut(s) 840
BstUI CGCG 1 cut(s) 1124
BstV1I GCAGC 4 cut(s) 154, 280, 830, 1232
BstV2I GAAGAC 4 cut(s) 135, 261, 487, 892
BstX2I RGATCY 1 cut(s) 1110
BstYI RGATCY 1 cut(s) 1110
BsuRI GGCC 4 cut(s) 5, 81, 812, 1197
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 2 cut(s) 159, 285
BtsIMutI CAGTG 2 cut(s) 159, 285
Cac8I GCNNGC 1 cut(s) 1120
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 1 cut(s) 1124
Cfr13I GGNCC 2 cut(s) 882, 1181
CviAII CATG 2 cut(s) 472, 1229
DdeI CTNAG 1 cut(s) 1188
DpnI GATC 6 cut(s) 432, 524, 951, 1026, 1112, 1193
DpnII GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
EaeI YGGCCR 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 868
Eco130I CCWWGG 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 870
Eco31I GGTCTC 1 cut(s) 351
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 2 cut(s) 882, 1181
Eco53kI GAGCTC 1 cut(s) 868
Eco57I CTGAAG 3 cut(s) 147, 273, 666
EcoICRI GAGCTC 1 cut(s) 868
EcoO109I RGGNCCY 1 cut(s) 882
EcoRII CCWGG 2 cut(s) 543, 1073
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 870
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 475, 1232
FaqI GGGAC 1 cut(s) 1031
FatI CATG 2 cut(s) 471, 1228
FauNDI CATATG 1 cut(s) 964
Fnu4HI GCNGC 5 cut(s) 82, 168, 294, 819, 1221
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 870
Fsp4HI GCNGC 5 cut(s) 82, 168, 294, 819, 1221
GlaI GCGC 1 cut(s) 1123
GluI GCNGC 5 cut(s) 82, 168, 294, 819, 1221
GsuI CTGGAG 2 cut(s) 378, 884
HaeIII GGCC 4 cut(s) 5, 81, 812, 1197
HapII CCGG 1 cut(s) 583
HhaI GCGC 1 cut(s) 1124
Hin1II CATG 2 cut(s) 475, 1232
Hin6I GCGC 1 cut(s) 1122
HinP1I GCGC 1 cut(s) 1122
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 2 cut(s) 59, 1156
HpaII CCGG 1 cut(s) 583
HphI GGTGA 3 cut(s) 668, 785, 959
Hpy166II GTNNAC 1 cut(s) 838
Hpy188I TCNGA 5 cut(s) 64, 221, 347, 527, 1189
Hpy188III TCNNGA 4 cut(s) 395, 863, 871, 904
Hpy8I GTNNAC 1 cut(s) 838
HpyAV CCTTC 4 cut(s) 641, 656, 671, 686
HpyCH4III ACNGT 3 cut(s) 890, 1133, 1217
HpyF10VI GCNNNNNNNGC 2 cut(s) 713, 818
HpyF3I CTNAG 1 cut(s) 1188
Hsp92II CATG 2 cut(s) 475, 1232
HspAI GCGC 1 cut(s) 1122
Kzo9I GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
LmnI GCTCC 4 cut(s) 410, 417, 444, 865
Lsp1109I GCAGC 4 cut(s) 154, 280, 830, 1232
LweI GCATC 4 cut(s) 30, 709, 805, 830
MaeIII GTNAC 2 cut(s) 113, 239
MalI GATC 6 cut(s) 432, 524, 951, 1026, 1112, 1193
MboI GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
MfeI CAATTG 1 cut(s) 16
MflI RGATCY 1 cut(s) 1110
MhlI GDGCHC 2 cut(s) 840, 870
MluCI AATT 5 cut(s) 16, 75, 499, 761, 1206
MmeI TCCRAC 5 cut(s) 199, 325, 977, 1107, 1149
MroXI GAANNNNTTC 1 cut(s) 577
MseI TTAA 3 cut(s) 725, 782, 1137
MspI CCGG 1 cut(s) 583
MspR9I CCNGG 3 cut(s) 545, 583, 1075
MunI CAATTG 1 cut(s) 16
Mva1269I GAATGC 1 cut(s) 1004
MvaI CCWGG 2 cut(s) 545, 1075
MvnI CGCG 1 cut(s) 1124
MwoI GCNNNNNNNGC 2 cut(s) 713, 818
NciI CCSGG 1 cut(s) 583
NdeI CATATG 1 cut(s) 964
NdeII GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
NlaIII CATG 2 cut(s) 475, 1232
NlaIV GGNNCC 2 cut(s) 440, 1214
NspI RCATGY 1 cut(s) 475
PciI ACATGT 1 cut(s) 471
PctI GAATGC 1 cut(s) 1004
PdmI GAANNNNTTC 1 cut(s) 577
PfeI GAWTC 2 cut(s) 59, 1156
PfoI TCCNGGA 1 cut(s) 581
PkrI GCNGC 5 cut(s) 83, 169, 295, 820, 1222
PpuMI RGGWCCY 1 cut(s) 882
PscI ACATGT 1 cut(s) 471
Psp124BI GAGCTC 1 cut(s) 870
Psp5II RGGWCCY 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 543, 1073
PspGI CCWGG 2 cut(s) 543, 1073
PspN4I GGNNCC 2 cut(s) 440, 1214
PspPI GGNCC 2 cut(s) 882, 1181
PspPPI RGGWCCY 1 cut(s) 882
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 1 cut(s) 1110
SacI GAGCTC 1 cut(s) 870
SaqAI TTAA 3 cut(s) 725, 782, 1137
SatI GCNGC 5 cut(s) 82, 168, 294, 819, 1221
Sau3AI GATC 6 cut(s) 430, 522, 949, 1024, 1110, 1191
Sau96I GGNCC 2 cut(s) 882, 1181
ScrFI CCNGG 3 cut(s) 545, 583, 1075
SduI GDGCHC 2 cut(s) 840, 870
SetI ASST 9 cut(s) 363, 415, 591, 646, 775, 870, 887, 1149, 1225
SfaNI GCATC 4 cut(s) 30, 709, 805, 830
SinI GGWCC 2 cut(s) 882, 1181
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
Sse9I AATT 5 cut(s) 16, 75, 499, 761, 1206
SsiI CCGC 1 cut(s) 82
SspI AATATT 1 cut(s) 619
SstI GAGCTC 1 cut(s) 870
StyD4I CCNGG 3 cut(s) 543, 581, 1073
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 3 cut(s) 890, 1133, 1217
TaqI TCGA 3 cut(s) 44, 550, 1164
TasI AATT 5 cut(s) 16, 75, 499, 761, 1206
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 2 cut(s) 59, 1156
Tru1I TTAA 3 cut(s) 725, 782, 1137
Tru9I TTAA 3 cut(s) 725, 782, 1137
TscAI CASTG 2 cut(s) 159, 285
TseI GCWGC 4 cut(s) 167, 293, 818, 1220
TspDTI ATGAA 6 cut(s) 202, 328, 675, 690, 705, 1011
TspRI CASTG 2 cut(s) 159, 285
VneI GTGCAC 1 cut(s) 836
VpaK11BI GGWCC 2 cut(s) 882, 1181
XceI RCATGY 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.