Rh3BG155600

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001475
Physical Location & Seq
Reverse (-)
13257441 .. 13260764
3324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG155600.1

Sequence Viewer

Length: 399 bp
ATGGCTGTGCACGATGAGTGCAAATTGAAGTTTCTGGAGCTCAAGACAAAGAGGACCTACCGATCAATAGTGTACAAGATCGAGGAGAAGCAAAAGCAAGTTATTGTGGAAGCAACTGGGGATCCAACACAAACCTATGAGAATTTCACAGATAGCCTTCCTTCTGATGAATGCCGTTATGCTGTTTTTGATTTCGATTTTCTTACACCAGAGGGTGTCCCAAAAAGCAGGATATTCTTCATTGCCTGGTCTCCTGATACATCAAGGGTGAGAAACAAGATGATCTATGCAAGCTCAAAGGACAGATTCAAGAGAGAGCTTGATGGAATTCAGATCGAGTTGCAGGCAACTGATCCATCTGAGATTGGCCTTGATGTTATTAAGAGCCGTGCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

132

Amino Acids

15.31

Weight (kDa)

5.9

Isoelectric Point (pI)

56.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 2 - 131 1.8e-38 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 116, 129, 347
AcsI RAATTY 2 cut(s) 142, 327
AfaI GTAC 1 cut(s) 74
AgsI TTSAA 2 cut(s) 28, 310
AjnI CCWGG 1 cut(s) 245
AluBI AGCT 4 cut(s) 40, 294, 319, 396
AluI AGCT 4 cut(s) 40, 294, 319, 396
Alw21I GWGCWC 2 cut(s) 12, 42
Alw26I GTCTC 1 cut(s) 255
Alw44I GTGCAC 1 cut(s) 8
AlwI GGATC 3 cut(s) 116, 129, 347
AoxI GGCC 1 cut(s) 367
ApaLI GTGCAC 1 cut(s) 8
ApoI RAATTY 2 cut(s) 142, 327
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 280
AvaII GGWCC 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 12
BamHI GGATCC 1 cut(s) 121
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 12, 42
BccI CCATC 2 cut(s) 317, 364
BceAI ACGGC 2 cut(s) 159, 372
BciT130I CCWGG 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 255
Bse1I ACTGG 1 cut(s) 121
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 247
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 12
BshFI GGCC 1 cut(s) 369
BsiHKAI GWGCWC 2 cut(s) 12, 42
BslFI GGGAC 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 1 cut(s) 369
Bso31I GGTCTC 1 cut(s) 255
Bsp1286I GDGCHC 2 cut(s) 12, 42
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 6 cut(s) 62, 78, 121, 282, 333, 352
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 3 cut(s) 116, 129, 347
BspTNI GGTCTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 121
BssMI GATC 6 cut(s) 62, 78, 121, 282, 333, 352
Bst2UI CCWGG 1 cut(s) 247
BstAUI TGTACA 1 cut(s) 72
BstC8I GCNNGC 3 cut(s) 292, 345, 394
BstDEI CTNAG 1 cut(s) 360
BstKTI GATC 6 cut(s) 65, 81, 124, 285, 336, 355
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 6 cut(s) 62, 78, 121, 282, 333, 352
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNI CCWGG 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 292, 345, 394
Cfr13I GGNCC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 73
CviJI RGCY 8 cut(s) 5, 40, 156, 294, 319, 369, 387, 396
CviKI_1 RGCY 8 cut(s) 5, 40, 156, 294, 319, 369, 387, 396
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 1 cut(s) 360
DpnI GATC 6 cut(s) 64, 80, 123, 284, 335, 354
DpnII GATC 6 cut(s) 62, 78, 121, 282, 333, 352
Ecl136II GAGCTC 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 54
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 42
FaiI YATR 3 cut(s) 138, 180, 288
FaqI GGGAC 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 42
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 1 cut(s) 369
HinfI GANTC 1 cut(s) 306
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 2 cut(s) 10, 73
Hpy188I TCNGA 3 cut(s) 166, 333, 361
Hpy188III TCNNGA 4 cut(s) 35, 43, 254, 310
Hpy8I GTNNAC 2 cut(s) 10, 73
HpyAV CCTTC 2 cut(s) 167, 171
HpyCH4V TGCA 4 cut(s) 10, 21, 290, 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 1 cut(s) 360
Kzo9I GATC 6 cut(s) 62, 78, 121, 282, 333, 352
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 8 cut(s) 20, 102, 214, 222, 232, 259, 267, 329
MalI GATC 6 cut(s) 64, 80, 123, 284, 335, 354
MboI GATC 6 cut(s) 62, 78, 121, 282, 333, 352
MboII GAAGA 1 cut(s) 229
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 2 cut(s) 12, 42
MluCI AATT 3 cut(s) 23, 142, 327
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 3 cut(s) 45, 76, 205
MseI TTAA 1 cut(s) 381
MspR9I CCNGG 1 cut(s) 247
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 393
NdeII GATC 6 cut(s) 62, 78, 121, 282, 333, 352
NlaIV GGNNCC 1 cut(s) 123
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 306
PpuMI RGGWCCY 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 245
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 54
PspPPI RGGWCCY 1 cut(s) 54
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 1 cut(s) 381
Sau3AI GATC 6 cut(s) 62, 78, 121, 282, 333, 352
Sau96I GGNCC 1 cut(s) 54
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 2 cut(s) 12, 42
SetI ASST 6 cut(s) 42, 59, 137, 296, 321, 398
SinI GGWCC 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 3 cut(s) 23, 142, 327
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 245
TaqI TCGA 3 cut(s) 81, 195, 336
TasI AATT 3 cut(s) 23, 142, 327
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TspDTI ATGAA 2 cut(s) 183, 229
VneI GTGCAC 1 cut(s) 8
VpaK11BI GGWCC 1 cut(s) 54
XapI RAATTY 2 cut(s) 142, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.