MD07G1191600.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012338
Physical Location & Seq
Forward (+)
27145894 .. 27149230
3337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1191600.v1.1.491

Sequence Viewer

Length: 2004 bp
ATGGTTTTGTCTAACAGAAAGCTGAAGCAAAAGCTAAGAGAAAAACTAGCTCAATCGTTAGTTGAAACTGTAGCGAAGCCTAAAACCGACCCGACAAATGATGGGTCGGAAAATCCGGACCCGAAATCGACCCCCCGGTCGCTGAAAGTGCTTCTGGACTCCGCAACCCAGAAACCCAGATTGTCCAAGCGGGAAAAACGCAGAAAGATCCTGTCCTTGCGAGGTCCAGAGGTGGTCAGTGTGAATGACAGTGGTGGTAGTTTGGGGGGAAACAAGGGGGAGGAAGGGAAGATGGATGTGGGTTCAGAGGGTTTAGGGGTTGAGAAGAAGAAGGAGGAGGAGAAGAAGAGGAAGAGAGACGATGAGGAGGAAAAGGATGGAGATTTGAGTGCAGAGGAAAATGGGGTTGTGAAGAAGGAGAGTAAGAAGCCAAAGAAGAAGAGTAAAACGAAGAAGAAGAAGAGAAAGACTGGGGCGAAAACCGAGGAAGAGAAGAAGGGTGGTGAGGTTGGGAATGGAGAGCAAGTTGTCCAGGAAACAGATATAAACATTGATAGGCAAGCGAGTGGGGATGTTCCGACAAAAGTTTATGTGGGAGGCATTCCTTATTACTCGACCGAGGATGATATTCGAAGTTACTTTGAAAGCTGCGGTACGATAACTGAAGTTGATTGTCTGAGGTTTCCCGACAGCGGGAAGTTTAGAGGAATTGCTATTATTAGCTTTAAGACAGAAGCAGCAGCTAAGCGTGCTTTGGCTCTTGATGGAGCTGAAATGGGTGGACTGTTTCTGAAAATCCAGCCATACAAGGCAACTCGACCCAATAAACCAAATAAAGTATCTGATTTTGCCCCCCAAATTGTGGAGGGATACAATAGAATCTATGTTGGAAATTTGTCATGGGATATTACTGAGGATGATCTAAAGAAACTTTTCTCAGATTGCAAGATATCATCTATACATTTTGGTATGGATAAGGAAACAGGGGAATTTCGAGGTTATGCTCATGTAAATTTCTCTGATAGTCTCTCGGTGACGATGGCATTGAAGCTAGATCAGAAGGTTGTATGTGGAAGACCTGTCAAGATAAGCTGTGCAGTTCCTCTGAAAAAAGCAGGGACTCCTTCAACTCCTGCACTTACAACACCAAGTACCCATTCAACATCTGTAGCTTCAGCTATGACTACTGATTCCATACCCTTAAATACTCCTTTAGGTACAGAGGCTGATGACGGTGGGTTGAGTGCCAGTAAGATTAAGAGGAGGACGTGCTATGAGTGTGGTGAGAAGGGCCATCTTTCTTCGGCTTGTTCAAAGTCTGCAACTACAATATTAAGTACCCATTCAATACCACTAGCTACAACTACCAGTACTGATCCGACACCAGTTGCTACAGAGTCAGGTAAAGGAGCTGATAATGGTGGGTTGAGTGCCACCAGTGGTAAGATTAAGAGGAGGACGTGCTATGAGTGTGGTGAGAAGGGTCATCTTTCTTCAGCTTGTCCAAAGTCTGCAACTACAGCTTTTAATACCCATTCAACACCAGTAGCTAAAACCACAAGTACCGATCCGACACCAGTTGCTACAGCTACAGGAGCTGATAATGGTGGGTTGAGTGACATCAGTGGTAAGATTAAGAGGAGGACGTGCTTTGAGTGTGGTGAAAAGGGTCATCTTTCTTCAGCTTGTCCAAAGGCTGGTACTACACCTTCAAGTACCCATTCAATTCCAGTAGCTACAGTTACAAGTACCAGTCCAATACCAGTAGCTACAACTACAAGCACCACTTGGATACCAGTTGCTACAACAAGCAGCACCAATTTGCCACCAGATGATACAACTACAGGCACCGGAGCTGATACTAGTGGGTTGAGTGCGAGCATTGGTAAGATTAAGAGGAGGACGTGCTATGAATGCGGTGAGAAGGGACATATTTCTTCAGCTTGTCCAAAGTGCAAGTTAAGCATGTCACCTGCAGGCAGTATCCCAAGTTTTTCCTATTAA

Protein Analysis

668

Amino Acids

70.83

Weight (kDa)

9.11

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 196 - 264 6.2e-13 RNA recognition motif
RRM_1 PF00076 294 - 361 6.5e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55340
fragaria_vesca FvH4_7g21531
malus_domestica MD01G1122100.v1.1 MD07G1191600.v1.1
prunus_persica Prupe.2G229600_v2.0.a1 Prupe.2G229700_v2.0.a1
pyrus_communis pycom01g14890 pycom07g18100
rosa_chinensis RchiOBHm_Chr1g0365851
rosa_laevigata RLG00000027386
rosa_multiflora Rmu_sc0023137.1_g000001 Rmu_sc0041703.1_g000001
rosa_roxburghii Rroxscaffold_4G00290800
rosa_rugosa Rorug01G0325000
rosa_samantha Rh1AG334000 Rh1BG294600 Rh1CG311300 Rh1DG327000
rosa_wichuraiana Rw1G029630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1981
AasI GACNNNNNNGTC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 1981
AccB1I GGYRCC 1 cut(s) 1847
AccB7I CCANNNNNTGG 2 cut(s) 862, 1697
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 5 cut(s) 162, 190, 651, 693, 1917
AclWI GGATC 3 cut(s) 202, 1370, 1562
AcsI RAATTY 3 cut(s) 892, 989, 1012
AcuI CTGAAG 6 cut(s) 44, 684, 1158, 1479, 1665, 1923
AfaI GTAC 9 cut(s) 655, 1153, 1219, 1339, 1372, 1564, 1702, 1717, 1750
AfiI CCNNNNNNNGG 4 cut(s) 692, 693, 862, 1697
AhlI ACTAGT 1 cut(s) 1862
AjiI CACGTC 4 cut(s) 1269, 1461, 1647, 1905
AjnI CCWGG 1 cut(s) 531
AjuI GAANNNNNNNTTGG 2 cut(s) 1141, 1173
Alw26I GTCTC 2 cut(s) 351, 1031
AlwI GGATC 3 cut(s) 202, 1370, 1562
AlwNI CAGNNNCTG 2 cut(s) 1226, 1598
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 1 cut(s) 1291
ApeKI GCWGC 4 cut(s) 648, 737, 740, 1812
ApoI RAATTY 3 cut(s) 892, 989, 1012
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 932
AspS9I GGNCC 3 cut(s) 118, 224, 1291
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 7 cut(s) 515, 1045, 1295, 1487, 1673, 1931, 1962
AsuII TTCGAA 1 cut(s) 631
AvaII GGWCC 2 cut(s) 118, 224
BanI GGYRCC 1 cut(s) 1847
BarI GAAGNNNNNNTAC 2 cut(s) 1693, 1725
BbsI GAAGAC 1 cut(s) 1081
BbvI GCAGC 4 cut(s) 635, 749, 752, 1824
BccI CCATC 6 cut(s) 95, 286, 371, 758, 1033, 1302
BciT130I CCWGG 1 cut(s) 533
BciVI GTATCC 3 cut(s) 863, 1785, 1994
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 351, 1031
BcuI ACTAGT 1 cut(s) 1862
BfaI CTAG 4 cut(s) 47, 1052, 1355, 1863
BfmI CTRYAG 9 cut(s) 69, 1167, 1392, 1518, 1584, 1590, 1737, 1842, 1974
BfuAI ACCTGC 1 cut(s) 1981
BfuI GTATCC 3 cut(s) 863, 1785, 1994
BisI GCNGC 4 cut(s) 649, 738, 741, 1813
BlpI GCTNAGC 1 cut(s) 744
BlsI GCNGC 4 cut(s) 650, 739, 742, 1814
BmcAI AGTACT 1 cut(s) 1372
Bme1390I CCNGG 2 cut(s) 136, 533
Bme18I GGWCC 2 cut(s) 118, 224
BmgBI CACGTC 4 cut(s) 1269, 1461, 1647, 1905
BmgT120I GGNCC 3 cut(s) 118, 224, 1291
BmiI GGNNCC 2 cut(s) 120, 1849
BmrFI CCNGG 2 cut(s) 136, 533
BmrI ACTGGG 1 cut(s) 480
BmuI ACTGGG 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 1081
Bpu1102I GCTNAGC 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 631
BpuMI CCSGG 1 cut(s) 136
BsaJI CCNNGG 3 cut(s) 134, 483, 618
BsaWI WCCGGW 2 cut(s) 115, 1850
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 692, 693, 862, 1697
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 3 cut(s) 134, 483, 618
BseGI GGATG 5 cut(s) 301, 382, 577, 628, 922
BseLI CCNNNNNNNGG 4 cut(s) 692, 693, 862, 1697
BseMII CTCAG 3 cut(s) 668, 903, 951
BseRI GAGGAG 7 cut(s) 350, 353, 380, 1276, 1468, 1654, 1912
BseXI GCAGC 4 cut(s) 635, 749, 752, 1824
BsgI GTGCAG 3 cut(s) 411, 1116, 1119
Bsh1285I CGRYCG 2 cut(s) 140, 618
BshFI GGCC 1 cut(s) 1293
BshNI GGYRCC 1 cut(s) 1847
BsiEI CGRYCG 2 cut(s) 140, 618
BsiSI CCGG 3 cut(s) 116, 136, 1851
BslFI GGGAC 2 cut(s) 1132, 1941
BslI CCNNNNNNNGG 4 cut(s) 692, 693, 862, 1697
BsmAI GTCTC 2 cut(s) 351, 1031
BsmBI CGTCTC 1 cut(s) 351
BsmFI GGGAC 2 cut(s) 1132, 1941
BsmI GAATGC 2 cut(s) 600, 1919
BsnI GGCC 1 cut(s) 1293
Bsp119I TTCGAA 1 cut(s) 631
Bsp13I TCCGGA 1 cut(s) 115
Bsp143I GATC 5 cut(s) 207, 919, 1054, 1375, 1567
Bsp1720I GCTNAGC 1 cut(s) 744
BspACI CCGC 5 cut(s) 162, 190, 651, 693, 1917
BspANI GGCC 1 cut(s) 1293
BspCNI CTCAG 3 cut(s) 669, 904, 950
BspEI TCCGGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 120, 1849
BspMAI CTGCAG 1 cut(s) 1978
BspMI ACCTGC 1 cut(s) 1981
BspPI GGATC 3 cut(s) 202, 1370, 1562
BspT104I TTCGAA 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 1847
BssECI CCNNGG 3 cut(s) 134, 483, 618
BssMI GATC 5 cut(s) 207, 919, 1054, 1375, 1567
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 5 cut(s) 70, 251, 786, 1235, 1741
Bst6I CTCTTC 5 cut(s) 341, 347, 434, 455, 483
BstBI TTCGAA 1 cut(s) 631
BstC8I GCNNGC 4 cut(s) 561, 750, 1879, 1978
BstDEI CTNAG 5 cut(s) 35, 677, 744, 912, 937
BstF5I GGATG 5 cut(s) 301, 382, 577, 628, 922
BstKTI GATC 5 cut(s) 210, 922, 1057, 1378, 1570
BstMAI GTCTC 2 cut(s) 351, 1031
BstMBI GATC 5 cut(s) 207, 919, 1054, 1375, 1567
BstMCI CGRYCG 2 cut(s) 140, 618
BstMWI GCNNNNNNNGC 6 cut(s) 148, 749, 1520, 1595, 1914, 1962
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 1 cut(s) 1969
BstSCI CCNGG 2 cut(s) 134, 531
BstSFI CTRYAG 9 cut(s) 69, 1167, 1392, 1518, 1584, 1590, 1737, 1842, 1974
BstV1I GCAGC 4 cut(s) 635, 749, 752, 1824
BstV2I GAAGAC 1 cut(s) 1081
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BsuI GTATCC 3 cut(s) 863, 1785, 1994
BsuRI GGCC 1 cut(s) 1293
BtrI CACGTC 4 cut(s) 1269, 1461, 1647, 1905
BtsCI GGATG 5 cut(s) 301, 382, 577, 628, 922
BtsIMutI CAGTG 4 cut(s) 244, 256, 1444, 1630
BveI ACCTGC 1 cut(s) 1981
Cac8I GCNNGC 4 cut(s) 561, 750, 1879, 1978
CaiI CAGNNNCTG 2 cut(s) 1226, 1598
Cfr13I GGNCC 3 cut(s) 118, 224, 1291
Csp6I GTAC 9 cut(s) 654, 1152, 1218, 1338, 1371, 1563, 1701, 1716, 1749
CviAII CATG 3 cut(s) 900, 1007, 1966
CviQI GTAC 9 cut(s) 654, 1152, 1218, 1338, 1371, 1563, 1701, 1716, 1749
DdeI CTNAG 5 cut(s) 35, 677, 744, 912, 937
DpnI GATC 5 cut(s) 209, 921, 1056, 1377, 1569
DpnII GATC 5 cut(s) 207, 919, 1054, 1375, 1567
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 5 cut(s) 341, 347, 434, 455, 483
EarI CTCTTC 5 cut(s) 341, 347, 434, 455, 483
Eco32I GATATC 1 cut(s) 951
Eco47I GGWCC 2 cut(s) 118, 224
Eco57I CTGAAG 6 cut(s) 44, 684, 1158, 1479, 1665, 1923
EcoRII CCWGG 1 cut(s) 531
EcoRV GATATC 1 cut(s) 951
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 3 cut(s) 903, 1010, 1969
FalI AAGNNNNNCTT 2 cut(s) 1771, 1803
FaqI GGGAC 2 cut(s) 1132, 1941
FatI CATG 3 cut(s) 899, 1006, 1965
FauI CCCGC 2 cut(s) 183, 686
Fnu4HI GCNGC 4 cut(s) 649, 738, 741, 1813
FokI GGATG 5 cut(s) 308, 389, 584, 635, 929
Fsp4HI GCNGC 4 cut(s) 649, 738, 741, 1813
FspBI CTAG 4 cut(s) 47, 1052, 1355, 1863
GluI GCNGC 4 cut(s) 649, 738, 741, 1813
HaeIII GGCC 1 cut(s) 1293
HapII CCGG 3 cut(s) 116, 136, 1851
Hin1II CATG 3 cut(s) 903, 1010, 1969
HinfI GANTC 5 cut(s) 158, 879, 1120, 1190, 1397
HpaII CCGG 3 cut(s) 116, 136, 1851
HphI GGTGA 7 cut(s) 515, 1045, 1295, 1487, 1673, 1931, 1962
Hpy166II GTNNAC 1 cut(s) 782
Hpy188III TCNNGA 6 cut(s) 116, 155, 227, 686, 761, 1084
Hpy8I GTNNAC 1 cut(s) 782
HpyCH4III ACNGT 5 cut(s) 70, 251, 786, 1235, 1741
HpyCH4IV ACGT 4 cut(s) 1268, 1460, 1646, 1904
HpyCH4V TGCA 8 cut(s) 392, 945, 1097, 1136, 1322, 1514, 1956, 1976
HpyF10VI GCNNNNNNNGC 6 cut(s) 148, 749, 1520, 1595, 1914, 1962
HpyF3I CTNAG 5 cut(s) 35, 677, 744, 912, 937
HpySE526I ACGT 4 cut(s) 1268, 1460, 1646, 1904
Hsp92II CATG 3 cut(s) 903, 1010, 1969
Kpn2I TCCGGA 1 cut(s) 115
Kzo9I GATC 5 cut(s) 207, 919, 1054, 1375, 1567
LmnI GCTCC 4 cut(s) 767, 1409, 1595, 1853
Lsp1109I GCAGC 4 cut(s) 635, 749, 752, 1824
MaeI CTAG 4 cut(s) 47, 1052, 1355, 1863
MaeII ACGT 4 cut(s) 1268, 1460, 1646, 1904
MaeIII GTNAC 5 cut(s) 635, 1033, 1616, 1741, 1968
MalI GATC 5 cut(s) 209, 921, 1056, 1377, 1569
MboI GATC 5 cut(s) 207, 919, 1054, 1375, 1567
MflI RGATCY 1 cut(s) 207
MluCI AATT 7 cut(s) 708, 858, 892, 989, 1012, 1725, 1819
MlyI GAGTC 3 cut(s) 152, 1114, 1406
MmeI TCCRAC 5 cut(s) 87, 602, 868, 1403, 1595
MroI TCCGGA 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 932
MspA1I CMGCKG 1 cut(s) 693
MspI CCGG 3 cut(s) 116, 136, 1851
MspR9I CCNGG 2 cut(s) 136, 533
Mva1269I GAATGC 2 cut(s) 600, 1919
MvaI CCWGG 1 cut(s) 533
MwoI GCNNNNNNNGC 6 cut(s) 148, 749, 1520, 1595, 1914, 1962
NciI CCSGG 1 cut(s) 136
NdeII GATC 5 cut(s) 207, 919, 1054, 1375, 1567
NlaIII CATG 3 cut(s) 903, 1010, 1969
NlaIV GGNNCC 2 cut(s) 120, 1849
NmuCI GTSAC 3 cut(s) 1033, 1616, 1968
NspI RCATGY 1 cut(s) 1969
NspV TTCGAA 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 1981
PctI GAATGC 2 cut(s) 600, 1919
PdmI GAANNNNTTC 1 cut(s) 932
PfeI GAWTC 2 cut(s) 879, 1190
PflMI CCANNNNNTGG 2 cut(s) 862, 1697
PfoI TCCNGGA 1 cut(s) 531
PkrI GCNGC 4 cut(s) 650, 739, 742, 1814
PleI GAGTC 3 cut(s) 152, 1114, 1405
PpsI GAGTC 3 cut(s) 152, 1114, 1405
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 2 cut(s) 120, 1849
PspPI GGNCC 3 cut(s) 118, 224, 1291
PstI CTGCAG 1 cut(s) 1978
PstNI CAGNNNCTG 2 cut(s) 1226, 1598
PsuI RGATCY 1 cut(s) 207
RsaI GTAC 9 cut(s) 655, 1153, 1219, 1339, 1372, 1564, 1702, 1717, 1750
RsaNI GTAC 9 cut(s) 654, 1152, 1218, 1338, 1371, 1563, 1701, 1716, 1749
SatI GCNGC 4 cut(s) 649, 738, 741, 1813
Sau3AI GATC 5 cut(s) 207, 919, 1054, 1375, 1567
Sau96I GGNCC 3 cut(s) 118, 224, 1291
SbfI CCTGCAGG 1 cut(s) 1978
ScaI AGTACT 1 cut(s) 1372
SchI GAGTC 3 cut(s) 152, 1114, 1406
ScrFI CCNGG 2 cut(s) 136, 533
SdaI CCTGCAGG 1 cut(s) 1978
SfcI CTRYAG 9 cut(s) 69, 1167, 1392, 1518, 1584, 1590, 1737, 1842, 1974
SfuI TTCGAA 1 cut(s) 631
SinI GGWCC 2 cut(s) 118, 224
SpeI ACTAGT 1 cut(s) 1862
Sse8387I CCTGCAGG 1 cut(s) 1978
Sse9I AATT 7 cut(s) 708, 858, 892, 989, 1012, 1725, 1819
SsiI CCGC 5 cut(s) 162, 190, 651, 693, 1917
SspI AATATT 1 cut(s) 1332
SspMI CTAG 4 cut(s) 47, 1052, 1355, 1863
StyD4I CCNGG 2 cut(s) 134, 531
TaaI ACNGT 5 cut(s) 70, 251, 786, 1235, 1741
TaiI ACGT 4 cut(s) 1271, 1463, 1649, 1907
TaqI TCGA 5 cut(s) 128, 614, 631, 817, 994
TaqII GACCGA 1 cut(s) 632
TasI AATT 7 cut(s) 708, 858, 892, 989, 1012, 1725, 1819
TatI WGTACW 1 cut(s) 1370
TfiI GAWTC 2 cut(s) 879, 1190
TscAI CASTG 4 cut(s) 244, 256, 1444, 1630
TseFI GTSAC 3 cut(s) 1033, 1616, 1968
TseI GCWGC 4 cut(s) 648, 737, 740, 1812
Tsp45I GTSAC 3 cut(s) 1033, 1616, 1968
TspDTI ATGAA 1 cut(s) 1926
TspRI CASTG 4 cut(s) 244, 256, 1444, 1630
Van91I CCANNNNNTGG 2 cut(s) 862, 1697
VpaK11BI GGWCC 2 cut(s) 118, 224
XapI RAATTY 3 cut(s) 892, 989, 1012
XceI RCATGY 1 cut(s) 1969
XmnI GAANNNNTTC 1 cut(s) 932
XspI CTAG 4 cut(s) 47, 1052, 1355, 1863
ZrmI AGTACT 1 cut(s) 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.