MD01G1123800.v1.1

Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005246
Physical Location & Seq
Reverse (-)
23682430 .. 23687692
5263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1123800.v1.1.491

Sequence Viewer

Length: 3954 bp
ATGAATAATCTGAAGCTCTACTCGGAGGTCTCTCTGAATCTCCACTTACAATCCGAGGAGGAGCTCATACTCTTCTCCGCTTTCGACATCGAGCAGAATCGCTTGTTCTTCGCTTCCTCCGACAATAACATTTACGCCACTCACCTCTCGTCCATCCAGAATGAAAGGGCGTGGAGCAAAACCTCGATATCGGCGCAAGTTAATCAAATCGAATTGGATCCCAAGGACTTCATCACTTCATTTAGTTACCTCATGGAGAAAGAGGCGCTTTTGGTGGGAACTGGCAATGGGCTTCTGCTGCTGCATTCTGTGGGTGTTAATGCTACCCAAGTCGTTGGTGGTGTGGATGGCGGTGTCAAGTGCATTGCCCCCAGTCCGGATGGTGACCTTCTTGCCATAACTACTGGGTCTGGGCAGGTGCTGGTGATGACTCAGGATTGGGATTTGTTGTACGAGACTGCGCTTGAGGATCTACCGGAAGATGGCAACCACGTATGTGAACAGCAATTATCTTCTAAGTTCACATTTGAAAGCCCTATTTCTTGGCGTGGTGATGGGAAGTACTTTGTCACCCTAAGTGAGGTGCAGGATTTGGCATCCATGCTTAAGAGGCTTAAAATTTGGGAGCGACATTCGGGAGCAGTACATGCTGTATCTGAATCAAAGTCGTTCATTGGATCAGTTGTGGACTGGATGCCCAGTGGAGCAAAAATTGCAGCTGTTTATGATAGGAAATCGGAAAATGAGTGCCCCTCAATAGTTTTCTTTGAGAGGAATGGGTTAGAAAGAAACTTGTTTAGCATCAATGAGCAAACAAATGCAACCATAGAATTTCTCAAGTGGAATTGCAGCTCAGATCTGCTTGCTGCCGTTGTCCGATGTGAAAACTATGACTGCGTCAAGGTTTGGTATTTCAGTAACAACCACTGGTACTTGAAATCTGAAATTAGATACCCAAGACAGGATGGAGTGAGGTTTGTCTGGAATCCAACGAAGTCACTGCAGTTGTTATGTTGGACTCTTGGTGGCCAGATTACATCGTACGACTTCATATGGAATTCAGCTGTGATGGATGACTCTACTGCGCTAGTAATTGATGACTCCAAGATACTTGTAACGCCTCTTTCTTTATACATAATGCCACCTCCGATGTACTTATTCAGCCTTAAATTCCTGAGTGCTGTCCGGGACTTTGCTTTCTATTCCAAGAATTCTAGGAACTGTTTGGCTGCTTTTCTGTCAGATGGTTCTTTATGTGTCGTTGAGCTTCCTGCCACTGATACTTGGGAAGAATTAGAAGGCAAAGAATTTGCTGCTGAAGCATCCATTTCTGAATCACCATTTGGTTCATTTTTGCATATGACATGGTTGGACCCGCATAAAATTCTTGCTGTTTCTCACTACGGGTTCAGTCATAGTAATTACTTATCTCAAACTACATCAAGTGAGGATGGCGCCGGCTTTTATTTGCAGGAAGTTGAGCTTGTATGTTCTGAGAATCATGTCCCTGGTTTAGTGACATGCTCAGGCTGGCATGCAAAAGTTGCTGGCCAAAACTCTCTAGAAGAGATGATTATTTCAATTGCTCCTAATCCTGCTAGAAAAGGTTCGGCATTTGTCCAATTTGATGGTGGAAAGGTTTGCGAGTATGTCCCAAAGTTCGGTATTCCTAGAGGAGTTCCAAAACATGATTGGAGTTTCTCATCAACTTGCCCTTCTATGAGTGTGGTTCTAGTTGGAAACAGTGGGTCATTGGAACCTTTAATTTTTGGACTTGATGAGAGCTGTAGGCTGCACGTCAATGGGAAGATAATATGCAACAACTGCAGCAGTTTCTCTCTGTACTCAAATTTGGATGACCAAGTGATCACGCATATAGTTTTGGCAACTAAGCAGGATTGCCTTTTTATTGCTGACATCACAGATATATTTCATAGAGAGTTGGAAATAAAATATGAGAAACCCATCCAAGCTGGTAACAAGAAACGAGAAGATCATGGAGACTTCATAAACATATGGGAAAGAGGTGCAAAAGTTGTTGGAGTCCTGCATGGAGACGAAGCTGCTGTTATTTTGCAAACAACTCGGGGGAACCTAGAATCCATGTATCCAAGAAAATTGGTCCTGGCCTCTATTTGCAATGCATTGGTACAGAGGCACTTCAGAGACGCACTTCTCATGGTGAGGCGGCATAGGATAGATTTCAATGTCATTGTTGACTATTGTGGTTGGCAAGTGTTTCTCCAATCAGCTTCAGAATTTGTCAAGCAGGTTAATAACTTGAACTACATAACTGAGTTTGTTTGTGCCATAAACAACGAAAACATTATAGAGACACTGTATAAAAAATTTATTTCTCTACCCTTCCCGAAAGAAGCTAAAGATGACTCCAAGGGTTTTGATTCGAATAACAAGATACCTTCTGTGCTTCTGGCCATAAGGAAGGCTCTTGAGGAACATTCACCAGAAGTTCCTGCAAGGGAGCTTTGCATTTTAACCACTCTAGCTCGTAGTGACCCCCCTGCACTTGATGAGGCTTTGGAGAGAATAAAAGCTATCCGTGAAATGGAATTGTCAGGTTCCAATGACCAAAGGAGAATGTCGTACCCCTCTGCTGAAGAGGCTTTGAAGCATCTCCTGTGGTTATCTGATTCTGAGTCTGTGTATGAGGCTGCTTTAGGCCTTTACGATTTGAACCTTGCAGCTATGGTGGCATTGAACTCTCAGCGGGACCCTAAAGAGTTCCTTCCTTTTCTTCAACAATTGGAATCGATGCCAGTTACTTTAATGCGTTATAACATTGACCTTAGGCTGCACAGGTTTGAGAAAGCGCTCAAGCACATTGTTTCTGCAGGAGACGCTTGTTACTCAGATTCTCTGAATCTGATGAGGAAAAATCCTCAACTTTTCCCCTTGGGACATCAATTGATTGCTAATCCTGCCAAGAAGAAGCAAGTCCTTGAGGCCTGGGGAGATTATCTTAGTGGTGAAAAATGCTTCGATGATGCTGCTGCCACTTATTTGTGTTGTTCCAGTTTGGAGAAGGCTTTGAAGTCATACCGTGCTTGTGGTAATTGGAGTAAGGTGCTTACAGTTGCTGGGCTTCTAAAACTGAGTGAAGATGAGATCATGCAAATGGCTCATGAGCTTTGTGAAGAACTTCAAGCACTTGGTAAACCAAGGGAAGCAGCCAAAATTGCTCTGGAGTATTGTGGTGACGTTAACAATGGGATGAATTTGTTGATTAGCGCCAGGGATTGGGAGGAGGCTTTGAGAGTCGCTTTAATGCACAATAGACAGGATTTGGTCTCTGATGTGAAGAATGCATCCTTGGAATGTGCAAGCTTGCTCATTGGTGAATACGAGGAAGGTTTGGAGAAAGTAGGGAAGTACTTGGCTCGATACTTGGCTTTAAGACAAAGAAGGTTACTTCTTGCAGCAAAAATCAAGTCAGAGGAATGGTCAATGGACGATCTTGACGATGATACTGCTTCAGAAGCTAGCAGTAATTTCAGTGGAATGAGTGCTTACACCACGGGGACCAGGGGTAGCTCTGGTACTTCCACCCGATCAAGGGCAGCCAGCAAGGCAAGAGATGCCAGGCGCCAGAGAAAAAGAGGAAAAATCCGCCCCGGCAGTCCTGGCGAGGAACTGGCTCTGGCAGACCACTTGAAGGGAATGTCTCTAACGACTGGAGCTTTGCAAGAGCTAAAGTCTTTGTTACTTTCGCTTATGATGCTTGGCGAGGTTGAAATCGCAAGGAAGCTGCAAAGGGCTGGGGAAAACCTTCAACTGTCTCATATGGCAGCAGTCAAACTAACTGAAGATACAATATCCAGTGATAACACAGATGAGCATACGCAGACTTTGGATCATTACACACAAATTATCCGAAGTGAAGTGCAGAACTCCGAGGCTTTCTTTTGGCGATGTAAAGTATTTCTTTCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IKI3

Protein Analysis

1318

Amino Acids

147.41

Weight (kDa)

5.38

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ELP1_1st PF04762 1 - 157 1.6e-28 ELP1 first N-terminal beta-propeller
Beta-prop_ELP1_1st PF04762 165 - 330 1.3e-45 ELP1 first N-terminal beta-propeller
Beta-prop_ELP1_2nd PF23797 364 - 680 1.5e-45 ELP1 N-terminal second beta-propeller
A-sol_ELP1 PF23925 705 - 922 7.3e-48 ELP1 alpha-solenoid
TPR_ELP1 PF23878 931 - 1092 2.8e-27 ELP1 TPR domain
HB_ELP1 PF23936 1105 - 1262 4.5e-12 ELP1 three-helix bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2796
AarI CACCTGC 1 cut(s) 406
Acc36I ACCTGC 2 cut(s) 406, 2260
AccB1I GGYRCC 2 cut(s) 1454, 3603
AccB7I CCANNNNNTGG 1 cut(s) 3258
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 6 cut(s) 78, 351, 1376, 2188, 2728, 3628
AclWI GGATC 5 cut(s) 212, 225, 477, 685, 3879
AcoI YGGCCR 3 cut(s) 1027, 1549, 2433
AcuI CTGAAG 7 cut(s) 32, 1338, 2146, 2238, 2637, 3477, 3843
AcyI GRCGYC 2 cut(s) 1455, 3604
AfeI AGCGCT 1 cut(s) 2832
AflII CTTAAG 1 cut(s) 605
AjiI CACGTC 1 cut(s) 1798
AjnI CCWGG 7 cut(s) 1507, 2124, 2966, 3251, 3542, 3599, 3640
AjuI GAANNNNNNNTTGG 4 cut(s) 1326, 1358, 3314, 3346
AleI CACNNNNGTG 1 cut(s) 495
AloI GAACNNNNNNTCC 2 cut(s) 2084, 2116
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 5 cut(s) 212, 225, 477, 685, 3879
AlwNI CAGNNNCTG 2 cut(s) 421, 3098
Ama87I CYCGRG 1 cut(s) 2085
Aor13HI TCCGGA 1 cut(s) 376
Aor51HI AGCGCT 1 cut(s) 2832
AoxI GGCC 6 cut(s) 1027, 1549, 2127, 2433, 2680, 2964
ArsI GACNNNNNNTTYG 4 cut(s) 3434, 3466, 3682, 3714
Asp700I GAANNNNTTC 3 cut(s) 1606, 3159, 3786
AspLEI GCGC 8 cut(s) 196, 268, 463, 1087, 1457, 2833, 3251, 3606
AspS9I GGNCC 4 cut(s) 1372, 2122, 2731, 3540
AsuC2I CCSGG 2 cut(s) 1187, 3633
AsuII TTCGAA 1 cut(s) 2405
AsuNHI GCTAGC 1 cut(s) 3500
AvaI CYCGRG 1 cut(s) 2085
AvaII GGWCC 4 cut(s) 1372, 2122, 2731, 3540
AxyI CCTNAGG 1 cut(s) 2807
BaeGI GKGCMC 1 cut(s) 752
BalI TGGCCA 3 cut(s) 1029, 1551, 2435
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 2 cut(s) 1454, 3603
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BceAI ACGGC 1 cut(s) 854
BcgI CGANNNNNNTGC 8 cut(s) 982, 1016, 2066, 2100, 2990, 3024, 3470, 3504
BciT130I CCWGG 7 cut(s) 1509, 2126, 2968, 3253, 3544, 3601, 3642
BciVI GTATCC 1 cut(s) 2118
BclI TGATCA 1 cut(s) 1866
BcnI CCSGG 2 cut(s) 1187, 3633
BfaI CTAG 9 cut(s) 1088, 1215, 1562, 1599, 1671, 1733, 2096, 2504, 3501
BfmI CTRYAG 4 cut(s) 1001, 1786, 1825, 2850
BfoI RGCGCY 5 cut(s) 269, 1458, 2834, 3252, 3607
BfrI CTTAAG 1 cut(s) 605
BfuAI ACCTGC 2 cut(s) 406, 2260
BfuI GTATCC 1 cut(s) 2118
BglI GCCNNNNNGGC 1 cut(s) 3587
BglII AGATCT 1 cut(s) 856
BmcAI AGTACT 2 cut(s) 563, 3392
Bme1390I CCNGG 9 cut(s) 1187, 1509, 2126, 2968, 3253, 3544, 3601, 3633, 3642
Bme18I GGWCC 4 cut(s) 1372, 2122, 2731, 3540
BmeT110I CYCGRG 1 cut(s) 2085
BmgBI CACGTC 1 cut(s) 1798
BmgT120I GGNCC 4 cut(s) 1372, 2122, 2731, 3540
BmrFI CCNGG 9 cut(s) 1187, 1509, 2126, 2968, 3253, 3544, 3601, 3633, 3642
BmrI ACTGGG 3 cut(s) 366, 414, 693
BmtI GCTAGC 1 cut(s) 3504
BmuI ACTGGG 3 cut(s) 366, 414, 693
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BpmI CTGGAG 2 cut(s) 3224, 3714
Bpu10I CCTNAGC 1 cut(s) 1525
Bpu14I TTCGAA 1 cut(s) 2405
BpuEI CTTGAG 5 cut(s) 485, 821, 2471, 2819, 2981
BpuMI CCSGG 2 cut(s) 1187, 3633
Bsa29I ATCGAT 1 cut(s) 2771
BsaAI YACGTR 1 cut(s) 493
BsaHI GRCGYC 2 cut(s) 1455, 3604
BsaI GGTCTC 2 cut(s) 34, 3313
BsaWI WCCGGW 2 cut(s) 376, 475
Bse118I RCCGGY 1 cut(s) 1457
Bse21I CCTNAGG 1 cut(s) 2807
Bse3DI GCAATG 3 cut(s) 292, 363, 2146
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 7 cut(s) 1509, 2126, 2968, 3253, 3544, 3601, 3642
BseCI ATCGAT 1 cut(s) 2771
BseMI GCAATG 3 cut(s) 292, 363, 2146
BseRI GAGGAG 4 cut(s) 71, 74, 1689, 3278
BseSI GKGCMC 1 cut(s) 752
BseYI CCCAGC 2 cut(s) 3098, 3776
BsgI GTGCAG 5 cut(s) 605, 1778, 2508, 2798, 3923
BshFI GGCC 6 cut(s) 1029, 1551, 2129, 2435, 2682, 2966
BshNI GGYRCC 2 cut(s) 1454, 3603
BshVI ATCGAT 1 cut(s) 2771
BsiHKAI GWGCWC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 2085
BsiSI CCGG 5 cut(s) 377, 476, 1186, 1458, 3633
BsiWI CGTACG 1 cut(s) 1041
BslFI GGGAC 6 cut(s) 1202, 1490, 1637, 2744, 2931, 3553
BsmBI CGTCTC 3 cut(s) 2049, 2160, 2850
BsmFI GGGAC 6 cut(s) 1202, 1490, 1637, 2744, 2931, 3553
BsmI GAATGC 2 cut(s) 304, 3328
BsnI GGCC 6 cut(s) 1029, 1551, 2129, 2435, 2682, 2966
Bso31I GGTCTC 2 cut(s) 34, 3313
BsoBI CYCGRG 1 cut(s) 2085
Bsp119I TTCGAA 1 cut(s) 2405
Bsp1286I GDGCHC 2 cut(s) 66, 752
Bsp13I TCCGGA 1 cut(s) 376
BspACI CCGC 6 cut(s) 78, 351, 1376, 2188, 2728, 3628
BspANI GGCC 6 cut(s) 1029, 1551, 2129, 2435, 2682, 2966
BspDI ATCGAT 1 cut(s) 2771
BspEI TCCGGA 1 cut(s) 376
BspHI TCATGA 1 cut(s) 3142
BspMAI CTGCAG 3 cut(s) 1005, 1829, 2854
BspMI ACCTGC 2 cut(s) 406, 2260
BspOI GCTAGC 1 cut(s) 3504
BspPI GGATC 5 cut(s) 212, 225, 477, 685, 3879
BspT104I TTCGAA 1 cut(s) 2405
BspT107I GGYRCC 2 cut(s) 1454, 3603
BspTI CTTAAG 1 cut(s) 605
BspTNI GGTCTC 2 cut(s) 34, 3313
BsrDI GCAATG 3 cut(s) 292, 363, 2146
BsrFI RCCGGY 1 cut(s) 1457
BssAI RCCGGY 1 cut(s) 1457
BssNI GRCGYC 2 cut(s) 1455, 3604
BssT1I CCWWGG 5 cut(s) 222, 2391, 2913, 3179, 3330
Bst2UI CCWGG 7 cut(s) 1509, 2126, 2968, 3253, 3544, 3601, 3642
Bst4CI ACNGT 6 cut(s) 1223, 1745, 2340, 3062, 3094, 3795
Bst6I CTCTTC 3 cut(s) 77, 1560, 2613
BstACI GRCGYC 2 cut(s) 1455, 3604
BstAFI CTTAAG 1 cut(s) 605
BstAPI GCANNNNNTGC 5 cut(s) 647, 713, 1544, 1824, 3596
BstBAI YACGTR 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 2405
BstC8I GCNNGC 9 cut(s) 864, 1459, 1532, 1536, 1549, 3343, 3347, 3502, 3583
BstDSI CCRYGG 1 cut(s) 3534
BstEII GGTNACC 1 cut(s) 383
BstENI CCTNNNNNAGG 1 cut(s) 578
BstH2I RGCGCY 5 cut(s) 269, 1458, 2834, 3252, 3607
BstHHI GCGC 8 cut(s) 196, 268, 463, 1087, 1457, 2833, 3251, 3606
BstNI CCWGG 7 cut(s) 1509, 2126, 2968, 3253, 3544, 3601, 3642
BstNSI RCATGY 3 cut(s) 650, 1524, 1538
BstPI GGTNACC 1 cut(s) 383
BstSCI CCNGG 9 cut(s) 1185, 1507, 2124, 2966, 3251, 3542, 3599, 3631, 3640
BstSFI CTRYAG 4 cut(s) 1001, 1786, 1825, 2850
BstSLI GKGCMC 1 cut(s) 752
BstX2I RGATCY 3 cut(s) 217, 469, 856
BstXI CCANNNNNNTGG 2 cut(s) 335, 1628
BstYI RGATCY 3 cut(s) 217, 469, 856
Bsu15I ATCGAT 1 cut(s) 2771
Bsu36I CCTNAGG 1 cut(s) 2807
BsuI GTATCC 1 cut(s) 2118
BsuRI GGCC 6 cut(s) 1029, 1551, 2129, 2435, 2682, 2966
BsuTUI ATCGAT 1 cut(s) 2771
BtgI CCRYGG 1 cut(s) 3534
BtgZI GCGATG 1 cut(s) 3943
BtrI CACGTC 1 cut(s) 1798
BtsI GCAGTG 1 cut(s) 998
BtsIMutI CAGTG 8 cut(s) 706, 925, 998, 1275, 1750, 2336, 3520, 3844
BveI ACCTGC 2 cut(s) 406, 2260
Cac8I GCNNGC 9 cut(s) 864, 1459, 1532, 1536, 1549, 3343, 3347, 3502, 3583
CaiI CAGNNNCTG 2 cut(s) 421, 3098
CciI TCATGA 1 cut(s) 3142
CfoI GCGC 8 cut(s) 196, 268, 463, 1087, 1457, 2833, 3251, 3606
Cfr10I RCCGGY 1 cut(s) 1457
Cfr13I GGNCC 4 cut(s) 1372, 2122, 2731, 3540
ClaI ATCGAT 1 cut(s) 2771
CseI GACGC 3 cut(s) 886, 2177, 2867
DinI GGCGCC 2 cut(s) 1456, 3605
EaeI YGGCCR 3 cut(s) 1027, 1549, 2433
Eam1104I CTCTTC 3 cut(s) 77, 1560, 2613
EarI CTCTTC 3 cut(s) 77, 1560, 2613
EciI GGCGGA 1 cut(s) 3617
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 5 cut(s) 222, 2391, 2913, 3179, 3330
Eco147I AGGCCT 2 cut(s) 2682, 2966
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 2 cut(s) 34, 3313
Eco32I GATATC 1 cut(s) 189
Eco47I GGWCC 4 cut(s) 1372, 2122, 2731, 3540
Eco47III AGCGCT 1 cut(s) 2832
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 7 cut(s) 32, 1338, 2146, 2238, 2637, 3477, 3843
Eco81I CCTNAGG 1 cut(s) 2807
Eco88I CYCGRG 1 cut(s) 2085
Eco91I GGTNACC 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 578
EcoO109I RGGNCCY 1 cut(s) 2731
EcoO65I GGTNACC 1 cut(s) 383
EcoRI GAATTC 2 cut(s) 1057, 1210
EcoRII CCWGG 7 cut(s) 1507, 2124, 2966, 3251, 3542, 3599, 3640
EcoRV GATATC 1 cut(s) 189
EcoT14I CCWWGG 5 cut(s) 222, 2391, 2913, 3179, 3330
EcoT22I ATGCAT 2 cut(s) 2146, 3328
EcoT38I GRGCYC 1 cut(s) 66
EgeI GGCGCC 2 cut(s) 1456, 3605
EheI GGCGCC 2 cut(s) 1456, 3605
ErhI CCWWGG 5 cut(s) 222, 2391, 2913, 3179, 3330
Esp3I CGTCTC 3 cut(s) 2049, 2160, 2850
FalI AAGNNNNNCTT 7 cut(s) 252, 284, 1467, 1499, 2730, 2762, 3927
FaqI GGGAC 6 cut(s) 1202, 1490, 1637, 2744, 2931, 3553
FauI CCCGC 2 cut(s) 1383, 2721
FauNDI CATATG 4 cut(s) 1052, 1359, 2015, 3801
FbaI TGATCA 1 cut(s) 1866
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 9 cut(s) 1088, 1215, 1562, 1599, 1671, 1733, 2096, 2504, 3501
GlaI GCGC 8 cut(s) 195, 267, 462, 1086, 1456, 2832, 3250, 3605
GsaI CCCAGC 2 cut(s) 3102, 3780
GsuI CTGGAG 2 cut(s) 3224, 3714
HaeII RGCGCY 5 cut(s) 269, 1458, 2834, 3252, 3607
HaeIII GGCC 6 cut(s) 1029, 1551, 2129, 2435, 2682, 2966
HapII CCGG 5 cut(s) 377, 476, 1186, 1458, 3633
HgaI GACGC 3 cut(s) 886, 2177, 2867
HhaI GCGC 8 cut(s) 196, 268, 463, 1087, 1457, 2833, 3251, 3606
Hin1I GRCGYC 2 cut(s) 1455, 3604
Hin6I GCGC 8 cut(s) 194, 266, 461, 1085, 1455, 2831, 3249, 3604
HinP1I GCGC 8 cut(s) 194, 266, 461, 1085, 1455, 2831, 3249, 3604
HincII GTYRAC 2 cut(s) 2218, 3223
HindII GTYRAC 2 cut(s) 2218, 3223
HindIII AAGCTT 1 cut(s) 3343
HpaI GTTAAC 1 cut(s) 3223
HpaII CCGG 5 cut(s) 377, 476, 1186, 1458, 3633
Hpy166II GTNNAC 6 cut(s) 500, 522, 688, 2218, 3176, 3223
Hpy8I GTNNAC 6 cut(s) 500, 522, 688, 2218, 3176, 3223
HpyCH4III ACNGT 6 cut(s) 1223, 1745, 2340, 3062, 3094, 3795
HpyCH4IV ACGT 3 cut(s) 492, 1797, 3219
HpySE526I ACGT 3 cut(s) 492, 1797, 3219
Hsp92I GRCGYC 2 cut(s) 1455, 3604
HspAI GCGC 8 cut(s) 194, 266, 461, 1085, 1455, 2831, 3249, 3604
KasI GGCGCC 2 cut(s) 1454, 3603
KflI GGGWCCC 1 cut(s) 2731
Kpn2I TCCGGA 1 cut(s) 376
KroI GCCGGC 1 cut(s) 1457
KroNI GCCGGC 1 cut(s) 1459
Ksp22I TGATCA 1 cut(s) 1866
KspAI GTTAAC 1 cut(s) 3223
LmnI GCTCC 8 cut(s) 61, 174, 625, 638, 704, 1591, 2482, 3695
MaeI CTAG 9 cut(s) 1088, 1215, 1562, 1599, 1671, 1733, 2096, 2504, 3501
MaeII ACGT 3 cut(s) 492, 1797, 3219
MfeI CAATTG 3 cut(s) 1581, 2762, 2924
MflI RGATCY 3 cut(s) 217, 469, 856
MhlI GDGCHC 2 cut(s) 66, 752
MlsI TGGCCA 3 cut(s) 1029, 1551, 2435
MluNI TGGCCA 3 cut(s) 1029, 1551, 2435
Mly113I GGCGCC 2 cut(s) 1455, 3604
MlyI GAGTC 8 cut(s) 424, 1012, 1070, 1094, 2052, 2381, 2666, 3285
MmeI TCCRAC 7 cut(s) 144, 995, 1013, 1350, 1717, 1923, 2020
Mox20I TGGCCA 3 cut(s) 1029, 1551, 2435
Mph1103I ATGCAT 2 cut(s) 2146, 3328
MroI TCCGGA 1 cut(s) 376
MroNI GCCGGC 1 cut(s) 1457
MroXI GAANNNNTTC 3 cut(s) 1606, 3159, 3786
MscI TGGCCA 3 cut(s) 1029, 1551, 2435
MslI CAYNNNNRTG 3 cut(s) 495, 1800, 3134
Msp20I TGGCCA 3 cut(s) 1029, 1551, 2435
MspA1I CMGCKG 3 cut(s) 719, 1064, 2728
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 5 cut(s) 377, 476, 1186, 1458, 3633
MspR9I CCNGG 9 cut(s) 1187, 1509, 2126, 2968, 3253, 3544, 3601, 3633, 3642
MunI CAATTG 3 cut(s) 1581, 2762, 2924
Mva1269I GAATGC 2 cut(s) 304, 3328
MvaI CCWGG 7 cut(s) 1509, 2126, 2968, 3253, 3544, 3601, 3642
NaeI GCCGGC 1 cut(s) 1459
NarI GGCGCC 2 cut(s) 1455, 3604
NciI CCSGG 2 cut(s) 1187, 3633
NdeI CATATG 4 cut(s) 1052, 1359, 2015, 3801
NgoMIV GCCGGC 1 cut(s) 1457
NheI GCTAGC 1 cut(s) 3500
NmuCI GTSAC 6 cut(s) 383, 568, 996, 1516, 2513, 3215
NsiI ATGCAT 2 cut(s) 2146, 3328
NspI RCATGY 3 cut(s) 650, 1524, 1538
NspV TTCGAA 1 cut(s) 2405
OliI CACNNNNGTG 1 cut(s) 495
PaeI GCATGC 1 cut(s) 1538
PagI TCATGA 1 cut(s) 3142
PaqCI CACCTGC 1 cut(s) 406
PceI AGGCCT 2 cut(s) 2682, 2966
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 3477
PctI GAATGC 2 cut(s) 304, 3328
PdiI GCCGGC 1 cut(s) 1459
PdmI GAANNNNTTC 3 cut(s) 1606, 3159, 3786
Pfl23II CGTACG 1 cut(s) 1041
PflFI GACNNNGTC 1 cut(s) 896
PflMI CCANNNNNTGG 1 cut(s) 3258
PfoI TCCNGGA 1 cut(s) 1185
PleI GAGTC 8 cut(s) 424, 1012, 1070, 1094, 2051, 2381, 2665, 3284
PluTI GGCGCC 2 cut(s) 1458, 3607
PpsI GAGTC 8 cut(s) 424, 1012, 1070, 1094, 2051, 2381, 2665, 3284
Ppu21I YACGTR 1 cut(s) 493
PpuMI RGGWCCY 1 cut(s) 2731
PsiI TTATAA 1 cut(s) 2796
Psp124BI GAGCTC 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 2731
Psp6I CCWGG 7 cut(s) 1507, 2124, 2966, 3251, 3542, 3599, 3640
PspEI GGTNACC 1 cut(s) 383
PspFI CCCAGC 2 cut(s) 3098, 3776
PspGI CCWGG 7 cut(s) 1507, 2124, 2966, 3251, 3542, 3599, 3640
PspLI CGTACG 1 cut(s) 1041
PspPI GGNCC 4 cut(s) 1372, 2122, 2731, 3540
PspPPI RGGWCCY 1 cut(s) 2731
PstI CTGCAG 3 cut(s) 1005, 1829, 2854
PstNI CAGNNNCTG 2 cut(s) 421, 3098
PsuI RGATCY 3 cut(s) 217, 469, 856
PsyI GACNNNGTC 1 cut(s) 896
PvuII CAGCTG 2 cut(s) 719, 1064
RseI CAYNNNNRTG 3 cut(s) 495, 1800, 3134
SacI GAGCTC 1 cut(s) 66
Sau96I GGNCC 4 cut(s) 1372, 2122, 2731, 3540
ScaI AGTACT 2 cut(s) 563, 3392
SchI GAGTC 8 cut(s) 424, 1012, 1070, 1094, 2052, 2381, 2666, 3285
ScrFI CCNGG 9 cut(s) 1187, 1509, 2126, 2968, 3253, 3544, 3601, 3633, 3642
SduI GDGCHC 2 cut(s) 66, 752
SfcI CTRYAG 4 cut(s) 1001, 1786, 1825, 2850
SfoI GGCGCC 2 cut(s) 1456, 3605
SfuI TTCGAA 1 cut(s) 2405
SinI GGWCC 4 cut(s) 1372, 2122, 2731, 3540
SmiMI CAYNNNNRTG 3 cut(s) 495, 1800, 3134
SmlI CTYRAG 6 cut(s) 464, 605, 836, 2450, 2834, 2960
SmoI CTYRAG 6 cut(s) 464, 605, 836, 2450, 2834, 2960
SphI GCATGC 1 cut(s) 1538
SseBI AGGCCT 2 cut(s) 2682, 2966
SsiI CCGC 6 cut(s) 78, 351, 1376, 2188, 2728, 3628
SspDI GGCGCC 2 cut(s) 1454, 3603
SspMI CTAG 9 cut(s) 1088, 1215, 1562, 1599, 1671, 1733, 2096, 2504, 3501
SstI GAGCTC 1 cut(s) 66
StuI AGGCCT 2 cut(s) 2682, 2966
StyD4I CCNGG 9 cut(s) 1185, 1507, 2124, 2966, 3251, 3542, 3599, 3631, 3640
StyI CCWWGG 5 cut(s) 222, 2391, 2913, 3179, 3330
TaaI ACNGT 6 cut(s) 1223, 1745, 2340, 3062, 3094, 3795
TaiI ACGT 3 cut(s) 495, 1800, 3222
TaqI TCGA 8 cut(s) 84, 90, 185, 210, 2405, 2771, 3000, 3400
TatI WGTACW 5 cut(s) 561, 643, 1152, 1842, 3390
TauI GCSGC 1 cut(s) 2191
TscAI CASTG 8 cut(s) 706, 932, 1005, 1282, 1750, 2343, 3520, 3844
TseFI GTSAC 6 cut(s) 383, 568, 996, 1516, 2513, 3215
Tsp45I GTSAC 6 cut(s) 383, 568, 996, 1516, 2513, 3215
TspGWI ACGGA 1 cut(s) 2549
TspRI CASTG 8 cut(s) 706, 932, 1005, 1282, 1750, 2343, 3520, 3844
Tth111I GACNNNGTC 1 cut(s) 896
Van91I CCANNNNNTGG 1 cut(s) 3258
Vha464I CTTAAG 1 cut(s) 605
VpaK11BI GGWCC 4 cut(s) 1372, 2122, 2731, 3540
XagI CCTNNNNNAGG 1 cut(s) 578
XbaI TCTAGA 1 cut(s) 1561
XceI RCATGY 3 cut(s) 650, 1524, 1538
XcmI CCANNNNNNNNNTGG 4 cut(s) 335, 1628, 1689, 3199
XmnI GAANNNNTTC 3 cut(s) 1606, 3159, 3786
XspI CTAG 9 cut(s) 1088, 1215, 1562, 1599, 1671, 1733, 2096, 2504, 3501
ZrmI AGTACT 2 cut(s) 563, 3392
Zsp2I ATGCAT 2 cut(s) 2146, 3328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.