MD07G1193500.v1.1

Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005246
Physical Location & Seq
Reverse (-)
27325172 .. 27330684
5513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1193500.v1.1.491

Sequence Viewer

Length: 3963 bp
ATGAATAATCTGAAGCTCTACTCGGAGGTCTCTCTGAATCTCCACTTACAATCCGAAGAGGAGCTCATACTCTTCTCCGCATTCGACATTGAGCAGAATCGCTTGTTCTTAGCTTCCTCCGCCAATAACATCTACGCCACTCACCTCTCCTCCATCCAGAATGAAAGGGCGTGGAGCAAAACCTCGATATCGGCGCAAGTTAATCAAATCGAATTGGATCCCGAGGACTTCATCACTTCATTTAGTTACCTCATGGAGAAAGAGGCGCTCTTGGTGGGAACTGGCAATGGGCTTCTGCTGCTGCATTCTGTGGATGATAATGCCTCCCAAGTTGTTGGCGGAGTGGATGGCGGTGTTAAGTGCGTTGCGCCTAGTCCGGATGGTGACCTTCTTGCCATAACCACTGGGTCTGGGCAGGTGCTGGTTATGACTCAGGATTGGGATTTGTTGTACGAGACTGCGCTGGAGGATCTACCGGAAGATGGCAACCACGTATGTGAACAGCAATTATCTTCTAAGTTCACATTTGAAAGCCCTATTTCCTGGCGAGGTGATGGGAAGTACTTTGTCACACTAGGTGAGGTGCAGGATTCAACGTCCTTGGTTAAGAGGCTTAAAATTTGGGAGCGACATTCAGGAGCAGTACATGCTGTTTCTGAATCAAAGTCGTTCATGGGATCAGTTGTGGACTGGATGCCCAGTGGAGCAAAAATTGCAGCTGTTTATGATAGGAAAACAGAAAATGAGTGCCCCTCAATAGTTTTCTTTGAGAGAAATGGGTTAGAAAGAAACTTGTTTAGCATCAATGAGCAAACAAATGCAACCATAGAATTTCTCAAGTGGAATTGCAGTTCAGATCTTCTTGCTGCTGTTGTCAGATGTGAAAACTATGACTGTGTCAAGGTTTGGTATTTCAGTAACAACCACTGGTACTTAAAATCTGAAGTTAGATACCCAAGACAGGATGGTGTGAGGTTTGTCTGGAATCCAACAAAGTCACTGCAGTTATTATGTTGGACTCTTGGTGGCCAGATTACATCGTATGACTTCATATGGAATTCAGCTGTGATGGATGTCTCAACTGCGCTAGTAATTGATGACTCCAAGATACTTGTAACCCCTTTTTCTTTATACCTAATGCCACCACCCATGTACTTATTCAGCCTTAAATTCCCCAGTGCTGTCCGAGACTTCGCCTTCTATTCCAAGAATTCCAAGAATTCTAAGAACTGTGTGGCTGCTTTTCTATCAGATGGTTCTTTATGTGTCGTTGAGCTTCCTGCCACTGATACTTGGGAAGAATTAGAAGGCAAAGAGTTTTCTGTTGAAGCATCCATTTCTGAATCACCATTTGGTTCTTTTTTGCATATGACATGGTTGGACCCACATAAAATTCTTGCTGTTTCTCACTATGGGTTCAGTCATAGTAATTATTTATCTCGAACTACATCAAGTGCGGATGGTGCTGGCTTTTATTTGCAGGAAATTGAGCTTGTATGTTCTGAGAATCATGCCCCTGGTTCAGTGACATGCTCAGGCTGGCATGCAAAAGTTTCCAGCCAAAGCTCTCTGGAAGAGATGATTATATCAATTGCTCCTAATCCTGCTAGAAAAGGTTCAGCATTTGTCCAATTTGATGGTGGAAAGGTTTGCGAGTATGTCCCAAAGTTCGGCATTCCTAGAGGAGTTCCAAAACAGGATTGGAGTTTCTCATCAACTTGCCCTTCTATGAGTGTGGTTCTAGTTGGAAACAGTGGGCCATTGGAACCTTTAATTTTTGGCCTTGATGAGAGCTGTAGGCTGCACGTCAATGGGAAGATAATATGCAACAACTGCAACAGTTTCTCTTTGTACTCAAATTTGGATGACCAAGTGATCACGCATTTAGTTTTGGCAACTAAGCAGGATTGTCTTTTTATTGCTGACATCACAGATATATTGCATAGAGAGGTGGAAATAAAATTCGAGAAATCCATCCAAGCTGGTAACAAGAAACGAGAAGATCATGGAGACTCCATAAACATATGGGAAAGAGGTGCAAAAGTTGTTGGAGTTCTGCATGGAGATGAAGCTGCTGTTATTTTGCAAACAACTCGGGGGAACCTAGAATCCATTTATCCAAGAAAATTGGTCCTGGCTTCTATTTGCAATGCATTGGTACAGAGGCGTTTCAGAGATGCATTTCTCATGGTGAGGCGGCATAGGATAGATTTCAATATTATTGTTGACTATTGTAGTTGGCAAGTGTTTCTCCAATCAGCTTCAGAATTTGTCAAGCAGGTTAATAATTTGAACTACATAACTGAGTTTGTTTGTGCTATAAACAACGAAAACACTACAGAGACACTGTATAAATTTTTTATTTCTCTACCCTTCCCGAAAGAAGCTAAAGATGACTCCAAGGGTTTTGATTCTAATAACAAGATATCTTCTGTGCTTCTGGCCATAAGGAGAGCTCTTGAGGAACATTCGCAGGAAGTTCCTGCAAGGGAGCTTTGCATTTTAACCACTCTAGCCCGTAGCGACCCCCCTGCACTTGATGAGGCTTTGGAGAGAATAAAAGTTATCCGTGAAATGGAATTGTCAGGTTCCAATGACCAAAGGAGAATGTTGTACCCCTCTGCTGAAGAGGCTTTGAAGCATCTCCTTTGGTTATCTGATTCTGAGTCTGTTTATGAAGCTGCTTTAGGCCTTTACGATTTGAACCTTGCAGCTATGGTGGCATTGAACTCTCAGCGGGACCCTAAAGAATTCCTTCCTTTTCTTCAGGAATTGGAATCTATGCCAGTTACTTTGATGCGTTATAACATTGACCTTAGGCTGCACAGGTTTGAGAAAGCTCTCAAGCACATTGTTTCTGCAGGAGACACTTGTTATGCAGATTCTCTGAACCTGATGAAGAAAAATCCTCATCTTTTCCCCTTGGGACTTCAATTGATTGCTGATCCTGCCAAGAAGAAGCAAGTCCTCGAGGCCTGGGGAGACCATCTTAGTGGTGAAAAATGCTTCGATGATGCTGCCGCCACTTATTTGTGCTGTTCCAGTTTGGAAAAGGCTTTGAAGTCATACCATGCTTGTGGTAATTGGAGTAAGGTGCTTACAGTTGCTGGGCTTCTAAAACTGGGTGAAGATGAGATTATGCAAATGGCTCATGAGCTTTGTGAAGAACTTCAAGCACTTGGTAAACCAAGGGAAGCAGCCAAAATTGCTCTGGAGTATTGTGGTGACATTAACAATGGGACAAATTTGCTGATTAGTGCAAGGGACTGGGAGGAAGCATTGAGAGTCGCTTTTATGCACAATAGACAGGATTTAGTCTCTGATGTGAAGAATGCATCCTTGGAATGTGCAAGGTTGCTTGTTGGTGAATATGAGGAAGGTGTGGAGAAAGTAGGGAAGTACTTGGCTCGCTACTTGGCTTTAAGACAAAGAAGGTTACTTCTGGCAGCAAAAATCCAGTCAGAGGAACGGTCAATGATTGATCTTGATGATGATACTGCTTCAGAAGCTAGCAGTAATTTCAGTGGAATGAGTGCTTACACCACAGGGACCAGGTTCAGCTCTGGTACATCTGCCCGATCAAGTGCAGCTAGCAAGGCAAGAGACGCCAGGCGCCAGAGGAAAAGAGGGAAAATCCGTCCCGGAAGTCCTGGTGAGGAACTTGCTCTGGCTGACCACTTGAAGGGAATGTCTCTAACGACTGGAGCTTTGCAAGAGCTAAAATCTTTGTTGCTTTCGCTTGTCATGCTTGGCGAGGTTGAAACCGCAAGGAAGCTGCAGAGGGCTGCGGAAAACCTTCAACTGTCTCATATGGCAGCAGTCAAACTAACTGAAGATACAATATCCAGTGATATCATAGATGAGCGCACACAGACTTTGGATCATTACACACAAACTATCAGAAGTGAAGTGCAGAACTCCGAGGCTTTCTTCTGGCGGTGTAAGGTATTTCATTCCCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IKI3

Protein Analysis

1321

Amino Acids

147.62

Weight (kDa)

5.39

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ELP1_1st PF04762 1 - 157 5.9e-28 ELP1 first N-terminal beta-propeller
Beta-prop_ELP1_1st PF04762 165 - 330 4.2e-45 ELP1 first N-terminal beta-propeller
Beta-prop_ELP1_2nd PF23797 364 - 683 7.9e-49 ELP1 N-terminal second beta-propeller
A-sol_ELP1 PF23925 708 - 925 7e-49 ELP1 alpha-solenoid
TPR_ELP1 PF23878 934 - 1095 5.4e-25 ELP1 TPR domain
HB_ELP1 PF23936 1108 - 1266 1.1e-12 ELP1 three-helix bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2805
AarI CACCTGC 1 cut(s) 406
AbsI CCTCGAGG 1 cut(s) 2969
Acc36I ACCTGC 2 cut(s) 406, 2269
AccB1I GGYRCC 1 cut(s) 3612
AccIII TCCGGA 1 cut(s) 376
AclWI GGATC 6 cut(s) 212, 225, 477, 685, 2939, 3888
AcoI YGGCCR 2 cut(s) 1027, 2442
AcuI CTGAAG 7 cut(s) 32, 963, 2247, 2646, 2750, 3486, 3852
AcyI GRCGYC 2 cut(s) 3606, 3613
AdeI CACNNNGTG 1 cut(s) 578
AfiI CCNNNNNNNGG 6 cut(s) 482, 961, 1670, 2575, 3463, 3682
AjiI CACGTC 1 cut(s) 1807
AjnI CCWGG 7 cut(s) 542, 1516, 2133, 2975, 3551, 3608, 3649
AjuI GAANNNNNNNTTGG 4 cut(s) 1335, 1367, 3323, 3355
AleI CACNNNNGTG 1 cut(s) 495
AloI GAACNNNNNNTCC 4 cut(s) 835, 867, 2093, 2125
Alw21I GWGCWC 2 cut(s) 66, 2458
AlwI GGATC 6 cut(s) 212, 225, 477, 685, 2939, 3888
AlwNI CAGNNNCTG 2 cut(s) 421, 3107
Ama87I CYCGRG 3 cut(s) 221, 2094, 2969
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 6 cut(s) 1027, 1757, 1780, 2442, 2689, 2973
ArsI GACNNNNNNTTYG 4 cut(s) 3443, 3475, 3691, 3723
Asp700I GAANNNNTTC 4 cut(s) 1615, 2754, 3168, 3795
AspLEI GCGC 7 cut(s) 196, 268, 370, 463, 1087, 3615, 3867
AspS9I GGNCC 5 cut(s) 1381, 1757, 2131, 2740, 3549
AsuC2I CCSGG 1 cut(s) 3642
AsuNHI GCTAGC 2 cut(s) 3509, 3590
AvaI CYCGRG 3 cut(s) 221, 2094, 2969
AvaII GGWCC 4 cut(s) 1381, 2131, 2740, 3549
AxyI CCTNAGG 1 cut(s) 2816
BaeGI GKGCMC 1 cut(s) 752
BalI TGGCCA 2 cut(s) 1029, 2444
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 3612
BanII GRGCYC 2 cut(s) 66, 2458
Bbv12I GWGCWC 2 cut(s) 66, 2458
BcgI CGANNNNNNTGC 6 cut(s) 2075, 2109, 2513, 2547, 2999, 3033
BciT130I CCWGG 7 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3651
BclI TGATCA 1 cut(s) 1875
BcnI CCSGG 1 cut(s) 3642
BfmI CTRYAG 5 cut(s) 1001, 1795, 2337, 2859, 3776
BfoI RGCGCY 2 cut(s) 269, 3616
BfuAI ACCTGC 2 cut(s) 406, 2269
BglII AGATCT 1 cut(s) 856
BmcAI AGTACT 2 cut(s) 563, 3401
Bme1390I CCNGG 8 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3642, 3651
Bme18I GGWCC 4 cut(s) 1381, 2131, 2740, 3549
BmeT110I CYCGRG 3 cut(s) 221, 2094, 2969
BmgBI CACGTC 1 cut(s) 1807
BmgT120I GGNCC 5 cut(s) 1381, 1757, 2131, 2740, 3549
BmiI GGNNCC 9 cut(s) 219, 1383, 1767, 2102, 2590, 2741, 2742, 3550, 3614
BmrFI CCNGG 8 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3642, 3651
BmrI ACTGGG 5 cut(s) 414, 693, 1170, 3131, 3277
BmsI GCATC 8 cut(s) 684, 810, 1340, 2167, 2650, 2787, 3004, 3344
BmtI GCTAGC 2 cut(s) 3513, 3594
BmuI ACTGGG 5 cut(s) 414, 693, 1170, 3131, 3277
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BpmI CTGGAG 3 cut(s) 485, 3233, 3723
Bpu10I CCTNAGC 1 cut(s) 1534
BpuEI CTTGAG 3 cut(s) 821, 2480, 2828
BpuMI CCSGG 1 cut(s) 3642
BsaAI YACGTR 1 cut(s) 493
BsaHI GRCGYC 2 cut(s) 3606, 3613
BsaI GGTCTC 2 cut(s) 34, 2976
BsaJI CCNNGG 9 cut(s) 222, 600, 1516, 2400, 2922, 2976, 3188, 3339, 3921
BsaWI WCCGGW 2 cut(s) 376, 475
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 6 cut(s) 482, 961, 1670, 2575, 3463, 3682
Bse21I CCTNAGG 1 cut(s) 2816
Bse3DI GCAATG 2 cut(s) 292, 2155
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 7 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3651
BseDI CCNNGG 9 cut(s) 222, 600, 1516, 2400, 2922, 2976, 3188, 3339, 3921
BseLI CCNNNNNNNGG 6 cut(s) 482, 961, 1670, 2575, 3463, 3682
BseMI GCAATG 2 cut(s) 292, 2155
BseMII CTCAG 6 cut(s) 446, 1494, 1548, 2295, 2655, 2747
BseRI GAGGAG 3 cut(s) 74, 139, 1698
BseSI GKGCMC 1 cut(s) 752
BseYI CCCAGC 1 cut(s) 3107
BsgI GTGCAG 6 cut(s) 605, 1787, 2517, 2807, 3606, 3932
BshFI GGCC 6 cut(s) 1029, 1759, 1782, 2444, 2691, 2975
BshNI GGYRCC 1 cut(s) 3612
BsiHKAI GWGCWC 2 cut(s) 66, 2458
BsiHKCI CYCGRG 3 cut(s) 221, 2094, 2969
BsiSI CCGG 3 cut(s) 377, 476, 3642
BslFI GGGAC 7 cut(s) 1646, 2753, 2940, 3253, 3278, 3562, 3624
BslI CCNNNNNNNGG 6 cut(s) 482, 961, 1670, 2575, 3463, 3682
BsmBI CGTCTC 1 cut(s) 3597
BsmFI GGGAC 7 cut(s) 1646, 2753, 2940, 3253, 3278, 3562, 3624
BsmI GAATGC 4 cut(s) 80, 304, 1674, 3337
BsnI GGCC 6 cut(s) 1029, 1759, 1782, 2444, 2691, 2975
Bso31I GGTCTC 2 cut(s) 34, 2976
BsoBI CYCGRG 3 cut(s) 221, 2094, 2969
Bsp1286I GDGCHC 3 cut(s) 66, 752, 2458
Bsp13I TCCGGA 1 cut(s) 376
BspANI GGCC 6 cut(s) 1029, 1759, 1782, 2444, 2691, 2975
BspCNI CTCAG 6 cut(s) 445, 1495, 1547, 2296, 2656, 2746
BspEI TCCGGA 1 cut(s) 376
BspHI TCATGA 1 cut(s) 3151
BspLI GGNNCC 9 cut(s) 219, 1383, 1767, 2102, 2590, 2741, 2742, 3550, 3614
BspMAI CTGCAG 3 cut(s) 1005, 2863, 3780
BspMI ACCTGC 2 cut(s) 406, 2269
BspOI GCTAGC 2 cut(s) 3513, 3594
BspPI GGATC 6 cut(s) 212, 225, 477, 685, 2939, 3888
BspT107I GGYRCC 1 cut(s) 3612
BspTNI GGTCTC 2 cut(s) 34, 2976
BsrDI GCAATG 2 cut(s) 292, 2155
BssECI CCNNGG 9 cut(s) 222, 600, 1516, 2400, 2922, 2976, 3188, 3339, 3921
BssNI GRCGYC 2 cut(s) 3606, 3613
BssT1I CCWWGG 5 cut(s) 600, 2400, 2922, 3188, 3339
Bst2UI CCWGG 7 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3651
Bst4CI ACNGT 8 cut(s) 896, 1232, 1754, 1841, 2349, 3103, 3471, 3804
Bst6I CTCTTC 4 cut(s) 51, 77, 1569, 2622
BstACI GRCGYC 2 cut(s) 3606, 3613
BstAPI GCANNNNNTGC 3 cut(s) 647, 713, 1833
BstBAI YACGTR 1 cut(s) 493
BstC8I GCNNGC 6 cut(s) 1468, 1541, 1545, 3409, 3511, 3592
BstEII GGTNACC 1 cut(s) 383
BstH2I RGCGCY 2 cut(s) 269, 3616
BstHHI GCGC 7 cut(s) 196, 268, 370, 463, 1087, 3615, 3867
BstNI CCWGG 7 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3651
BstNSI RCATGY 3 cut(s) 650, 1533, 1547
BstPI GGTNACC 1 cut(s) 383
BstSCI CCNGG 8 cut(s) 542, 1516, 2133, 2975, 3551, 3608, 3640, 3649
BstSFI CTRYAG 5 cut(s) 1001, 1795, 2337, 2859, 3776
BstSLI GKGCMC 1 cut(s) 752
BstX2I RGATCY 3 cut(s) 217, 469, 856
BstXI CCANNNNNNTGG 4 cut(s) 335, 1637, 2993, 3077
BstYI RGATCY 3 cut(s) 217, 469, 856
Bsu36I CCTNAGG 1 cut(s) 2816
BsuRI GGCC 6 cut(s) 1029, 1759, 1782, 2444, 2691, 2975
BtrI CACGTC 1 cut(s) 1807
BtsI GCAGTG 1 cut(s) 998
BveI ACCTGC 2 cut(s) 406, 2269
Cac8I GCNNGC 6 cut(s) 1468, 1541, 1545, 3409, 3511, 3592
CaiI CAGNNNCTG 2 cut(s) 421, 3107
CciI TCATGA 1 cut(s) 3151
CfoI GCGC 7 cut(s) 196, 268, 370, 463, 1087, 3615, 3867
Cfr13I GGNCC 5 cut(s) 1381, 1757, 2131, 2740, 3549
CseI GACGC 1 cut(s) 3614
CsiI ACCWGGT 1 cut(s) 3551
DinI GGCGCC 1 cut(s) 3614
DraIII CACNNNGTG 1 cut(s) 578
EaeI YGGCCR 2 cut(s) 1027, 2442
Eam1104I CTCTTC 4 cut(s) 51, 77, 1569, 2622
EarI CTCTTC 4 cut(s) 51, 77, 1569, 2622
EciI GGCGGA 2 cut(s) 109, 354
Ecl136II GAGCTC 2 cut(s) 64, 2456
Eco130I CCWWGG 5 cut(s) 600, 2400, 2922, 3188, 3339
Eco147I AGGCCT 2 cut(s) 2691, 2975
Eco24I GRGCYC 2 cut(s) 66, 2458
Eco31I GGTCTC 2 cut(s) 34, 2976
Eco32I GATATC 3 cut(s) 189, 2427, 3853
Eco47I GGWCC 4 cut(s) 1381, 2131, 2740, 3549
Eco53kI GAGCTC 2 cut(s) 64, 2456
Eco57I CTGAAG 7 cut(s) 32, 963, 2247, 2646, 2750, 3486, 3852
Eco81I CCTNAGG 1 cut(s) 2816
Eco88I CYCGRG 3 cut(s) 221, 2094, 2969
Eco91I GGTNACC 1 cut(s) 383
EcoICRI GAGCTC 2 cut(s) 64, 2456
EcoO109I RGGNCCY 1 cut(s) 2740
EcoO65I GGTNACC 1 cut(s) 383
EcoRI GAATTC 4 cut(s) 1057, 1210, 1219, 2750
EcoRII CCWGG 7 cut(s) 542, 1516, 2133, 2975, 3551, 3608, 3649
EcoRV GATATC 3 cut(s) 189, 2427, 3853
EcoT14I CCWWGG 5 cut(s) 600, 2400, 2922, 3188, 3339
EcoT22I ATGCAT 3 cut(s) 2155, 2182, 3337
EcoT38I GRGCYC 2 cut(s) 66, 2458
EgeI GGCGCC 1 cut(s) 3614
EheI GGCGCC 1 cut(s) 3614
ErhI CCWWGG 5 cut(s) 600, 2400, 2922, 3188, 3339
Esp3I CGTCTC 1 cut(s) 3597
FalI AAGNNNNNCTT 2 cut(s) 2739, 2771
FaqI GGGAC 7 cut(s) 1646, 2753, 2940, 3253, 3278, 3562, 3624
FauI CCCGC 1 cut(s) 2730
FauNDI CATATG 4 cut(s) 1052, 1368, 2024, 3810
FbaI TGATCA 1 cut(s) 1875
FriOI GRGCYC 2 cut(s) 66, 2458
GlaI GCGC 7 cut(s) 195, 267, 369, 462, 1086, 3614, 3866
GsaI CCCAGC 1 cut(s) 3111
GsuI CTGGAG 3 cut(s) 485, 3233, 3723
HaeII RGCGCY 2 cut(s) 269, 3616
HaeIII GGCC 6 cut(s) 1029, 1759, 1782, 2444, 2691, 2975
HapII CCGG 3 cut(s) 377, 476, 3642
HgaI GACGC 1 cut(s) 3614
HhaI GCGC 7 cut(s) 196, 268, 370, 463, 1087, 3615, 3867
Hin1I GRCGYC 2 cut(s) 3606, 3613
Hin6I GCGC 7 cut(s) 194, 266, 368, 461, 1085, 3613, 3865
HinP1I GCGC 7 cut(s) 194, 266, 368, 461, 1085, 3613, 3865
HincII GTYRAC 1 cut(s) 2227
HindII GTYRAC 1 cut(s) 2227
HpaII CCGG 3 cut(s) 377, 476, 3642
Hpy166II GTNNAC 5 cut(s) 500, 522, 688, 2227, 3185
Hpy8I GTNNAC 5 cut(s) 500, 522, 688, 2227, 3185
HpyCH4III ACNGT 8 cut(s) 896, 1232, 1754, 1841, 2349, 3103, 3471, 3804
HpyCH4IV ACGT 3 cut(s) 492, 596, 1806
HpySE526I ACGT 3 cut(s) 492, 596, 1806
Hsp92I GRCGYC 2 cut(s) 3606, 3613
HspAI GCGC 7 cut(s) 194, 266, 368, 461, 1085, 3613, 3865
KasI GGCGCC 1 cut(s) 3612
KflI GGGWCCC 1 cut(s) 2740
Kpn2I TCCGGA 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 1875
LmnI GCTCC 8 cut(s) 61, 174, 625, 638, 704, 1600, 2491, 3704
LweI GCATC 8 cut(s) 684, 810, 1340, 2167, 2650, 2787, 3004, 3344
MabI ACCWGGT 1 cut(s) 3551
MaeII ACGT 3 cut(s) 492, 596, 1806
MfeI CAATTG 2 cut(s) 1590, 2933
MflI RGATCY 3 cut(s) 217, 469, 856
MhlI GDGCHC 3 cut(s) 66, 752, 2458
MlsI TGGCCA 2 cut(s) 1029, 2444
MluNI TGGCCA 2 cut(s) 1029, 2444
Mly113I GGCGCC 1 cut(s) 3613
MlyI GAGTC 7 cut(s) 424, 1012, 1094, 2006, 2390, 2675, 3294
MmeI TCCRAC 5 cut(s) 995, 1013, 1359, 1726, 2029
Mox20I TGGCCA 2 cut(s) 1029, 2444
Mph1103I ATGCAT 3 cut(s) 2155, 2182, 3337
MroI TCCGGA 1 cut(s) 376
MroXI GAANNNNTTC 4 cut(s) 1615, 2754, 3168, 3795
MscI TGGCCA 2 cut(s) 1029, 2444
MslI CAYNNNNRTG 5 cut(s) 495, 1809, 2064, 2991, 3075
Msp20I TGGCCA 2 cut(s) 1029, 2444
MspA1I CMGCKG 3 cut(s) 719, 1064, 2737
MspI CCGG 3 cut(s) 377, 476, 3642
MspR9I CCNGG 8 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3642, 3651
MunI CAATTG 2 cut(s) 1590, 2933
Mva1269I GAATGC 4 cut(s) 80, 304, 1674, 3337
MvaI CCWGG 7 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3651
NarI GGCGCC 1 cut(s) 3613
NciI CCSGG 1 cut(s) 3642
NdeI CATATG 4 cut(s) 1052, 1368, 2024, 3810
NheI GCTAGC 2 cut(s) 3509, 3590
NlaIV GGNNCC 9 cut(s) 219, 1383, 1767, 2102, 2590, 2741, 2742, 3550, 3614
NmuCI GTSAC 5 cut(s) 383, 568, 996, 1525, 3224
NsiI ATGCAT 3 cut(s) 2155, 2182, 3337
NspI RCATGY 3 cut(s) 650, 1533, 1547
OliI CACNNNNGTG 1 cut(s) 495
PaeI GCATGC 1 cut(s) 1547
PaeR7I CTCGAG 1 cut(s) 2969
PagI TCATGA 1 cut(s) 3151
PaqCI CACCTGC 1 cut(s) 406
PceI AGGCCT 2 cut(s) 2691, 2975
PctI GAATGC 4 cut(s) 80, 304, 1674, 3337
PdmI GAANNNNTTC 4 cut(s) 1615, 2754, 3168, 3795
PflFI GACNNNGTC 1 cut(s) 896
PfoI TCCNGGA 1 cut(s) 3640
PleI GAGTC 7 cut(s) 424, 1012, 1094, 2006, 2390, 2674, 3293
PluTI GGCGCC 1 cut(s) 3616
PpsI GAGTC 7 cut(s) 424, 1012, 1094, 2006, 2390, 2674, 3293
Ppu21I YACGTR 1 cut(s) 493
PpuMI RGGWCCY 1 cut(s) 2740
PsiI TTATAA 1 cut(s) 2805
Psp124BI GAGCTC 2 cut(s) 66, 2458
Psp5II RGGWCCY 1 cut(s) 2740
Psp6I CCWGG 7 cut(s) 542, 1516, 2133, 2975, 3551, 3608, 3649
PspEI GGTNACC 1 cut(s) 383
PspFI CCCAGC 1 cut(s) 3107
PspGI CCWGG 7 cut(s) 542, 1516, 2133, 2975, 3551, 3608, 3649
PspN4I GGNNCC 9 cut(s) 219, 1383, 1767, 2102, 2590, 2741, 2742, 3550, 3614
PspPI GGNCC 5 cut(s) 1381, 1757, 2131, 2740, 3549
PspPPI RGGWCCY 1 cut(s) 2740
PspXI VCTCGAGB 1 cut(s) 2969
PstI CTGCAG 3 cut(s) 1005, 2863, 3780
PstNI CAGNNNCTG 2 cut(s) 421, 3107
PsuI RGATCY 3 cut(s) 217, 469, 856
PsyI GACNNNGTC 1 cut(s) 896
PvuII CAGCTG 2 cut(s) 719, 1064
RseI CAYNNNNRTG 5 cut(s) 495, 1809, 2064, 2991, 3075
SacI GAGCTC 2 cut(s) 66, 2458
Sau96I GGNCC 5 cut(s) 1381, 1757, 2131, 2740, 3549
ScaI AGTACT 2 cut(s) 563, 3401
SchI GAGTC 7 cut(s) 424, 1012, 1094, 2006, 2390, 2675, 3294
ScrFI CCNGG 8 cut(s) 544, 1518, 2135, 2977, 3553, 3610, 3642, 3651
SduI GDGCHC 3 cut(s) 66, 752, 2458
SexAI ACCWGGT 1 cut(s) 3551
SfaNI GCATC 8 cut(s) 684, 810, 1340, 2167, 2650, 2787, 3004, 3344
SfcI CTRYAG 5 cut(s) 1001, 1795, 2337, 2859, 3776
SfoI GGCGCC 1 cut(s) 3614
Sfr274I CTCGAG 1 cut(s) 2969
SinI GGWCC 4 cut(s) 1381, 2131, 2740, 3549
SlaI CTCGAG 1 cut(s) 2969
SmiMI CAYNNNNRTG 5 cut(s) 495, 1809, 2064, 2991, 3075
SmlI CTYRAG 4 cut(s) 836, 2459, 2843, 2969
SmoI CTYRAG 4 cut(s) 836, 2459, 2843, 2969
SphI GCATGC 1 cut(s) 1547
SseBI AGGCCT 2 cut(s) 2691, 2975
SspDI GGCGCC 1 cut(s) 3612
SspI AATATT 1 cut(s) 2218
SstI GAGCTC 2 cut(s) 66, 2458
StuI AGGCCT 2 cut(s) 2691, 2975
StyD4I CCNGG 8 cut(s) 542, 1516, 2133, 2975, 3551, 3608, 3640, 3649
StyI CCWWGG 5 cut(s) 600, 2400, 2922, 3188, 3339
TaaI ACNGT 8 cut(s) 896, 1232, 1754, 1841, 2349, 3103, 3471, 3804
TaiI ACGT 3 cut(s) 495, 599, 1809
TaqI TCGA 7 cut(s) 84, 185, 210, 1441, 1965, 2970, 3009
TatI WGTACW 5 cut(s) 561, 643, 1152, 1851, 3399
TauI GCSGC 2 cut(s) 2200, 3023
TseFI GTSAC 5 cut(s) 383, 568, 996, 1525, 3224
Tsp45I GTSAC 5 cut(s) 383, 568, 996, 1525, 3224
TspGWI ACGGA 2 cut(s) 2558, 3626
Tth111I GACNNNGTC 1 cut(s) 896
VpaK11BI GGWCC 4 cut(s) 1381, 2131, 2740, 3549
XceI RCATGY 3 cut(s) 650, 1533, 1547
XcmI CCANNNNNNNNNTGG 3 cut(s) 1637, 1698, 3208
XhoI CTCGAG 1 cut(s) 2969
XmnI GAANNNNTTC 4 cut(s) 1615, 2754, 3168, 3795
ZrmI AGTACT 2 cut(s) 563, 3401
Zsp2I ATGCAT 3 cut(s) 2155, 2182, 3337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.