MD01G1132600.v1.1

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004292
Physical Location & Seq
Reverse (-)
24250011 .. 24252354
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1132600.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGGGAGGCTATTTGTGGTGAGTCTCGAGGGGAAGATCTACAGCTGCAGGCACTGCCGGACCCATCTTGCTCTTTGTGAGGACATAGTTTCCAGGTCTTTCCACTCCAGGCACGGGAAGGCGTATCTCTTCAAGAAGGTAGTGAACGTATTTTCCGGAGAGTGTGAAGATAGATCGATGATCACTGGACTGCACACAGTTGCTGACATTTTCTGTGTCGGGTGTGGATCGATAGTGGGGTGGAAATATGAGACTGCCCACGAAGAGGCCCAGAAGTACAAGGAAGGAAAATCCGTACTTGAGCGGGTGAAGGTGTTGGGTCCGGATGGAAACAATTATTGGGTCAGTCACGAAGCACAAGTCGGTAGCAGCGATGCAGATGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

130

Amino Acids

14.4

Weight (kDa)

6.64

Isoelectric Point (pI)

18.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 13 - 104 1.2e-13 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 304
AccIII TCCGGA 2 cut(s) 155, 322
AciI CCGC 1 cut(s) 304
AclWI GGATC 1 cut(s) 235
AfaI GTAC 2 cut(s) 278, 297
AfiI CCNNNNNNNGG 2 cut(s) 114, 265
AgsI TTSAA 1 cut(s) 133
AjnI CCWGG 2 cut(s) 92, 107
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 2 cut(s) 45, 386
AluI AGCT 2 cut(s) 45, 386
Alw26I GTCTC 2 cut(s) 29, 245
AlwI GGATC 1 cut(s) 235
AlwNI CAGNNNCTG 2 cut(s) 54, 203
Ama87I CYCGRG 1 cut(s) 26
Aor13HI TCCGGA 2 cut(s) 155, 322
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 2 cut(s) 45, 369
ArsI GACNNNNNNTTYG 2 cut(s) 345, 377
AspS9I GGNCC 3 cut(s) 60, 268, 320
AsuHPI GGTGA 2 cut(s) 31, 319
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 2 cut(s) 60, 320
BbvI GCAGC 2 cut(s) 32, 381
BccI CCATC 2 cut(s) 72, 320
BciT130I CCWGG 2 cut(s) 94, 109
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 29, 245
BfmI CTRYAG 2 cut(s) 40, 46
BglII AGATCT 1 cut(s) 36
BisI GCNGC 2 cut(s) 46, 370
BlsI GCNGC 2 cut(s) 47, 371
Bme1390I CCNGG 2 cut(s) 94, 109
Bme18I GGWCC 2 cut(s) 60, 320
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 60, 268, 320
BmiI GGNNCC 2 cut(s) 62, 321
BmrFI CCNGG 2 cut(s) 94, 109
BmsI GCATC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 320
Bsa29I ATCGAT 2 cut(s) 176, 230
BsaWI WCCGGW 2 cut(s) 155, 322
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 265
Bse1I ACTGG 1 cut(s) 190
BseAI TCCGGA 2 cut(s) 155, 322
BseBI CCWGG 2 cut(s) 94, 109
BseCI ATCGAT 2 cut(s) 176, 230
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 2 cut(s) 114, 265
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 2 cut(s) 32, 381
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 269
BshVI ATCGAT 2 cut(s) 176, 230
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 58, 156, 323
BslI CCNNNNNNNGG 2 cut(s) 114, 265
BsmAI GTCTC 2 cut(s) 29, 245
BsnI GGCC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 26
Bsp13I TCCGGA 2 cut(s) 155, 322
Bsp143I GATC 4 cut(s) 36, 173, 180, 227
BspACI CCGC 1 cut(s) 304
BspANI GGCC 1 cut(s) 269
BspDI ATCGAT 2 cut(s) 176, 230
BspEI TCCGGA 2 cut(s) 155, 322
BspLI GGNNCC 2 cut(s) 62, 321
BspMAI CTGCAG 1 cut(s) 50
BspPI GGATC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 304
BsrI ACTGG 1 cut(s) 190
BssMI GATC 4 cut(s) 36, 173, 180, 227
Bst2UI CCWGG 2 cut(s) 94, 109
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 2 cut(s) 134, 258
BstAPI GCANNNNNTGC 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 50
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 4 cut(s) 39, 176, 183, 230
BstMAI GTCTC 2 cut(s) 29, 245
BstMBI GATC 4 cut(s) 36, 173, 180, 227
BstMWI GCNNNNNNNGC 2 cut(s) 54, 383
BstNI CCWGG 2 cut(s) 94, 109
BstSCI CCNGG 2 cut(s) 92, 107
BstSFI CTRYAG 2 cut(s) 40, 46
BstV1I GCAGC 2 cut(s) 32, 381
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
Bsu15I ATCGAT 2 cut(s) 176, 230
BsuRI GGCC 1 cut(s) 269
BsuTUI ATCGAT 2 cut(s) 176, 230
BtsCI GGATG 1 cut(s) 331
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 2 cut(s) 52, 183
Cac8I GCNNGC 1 cut(s) 50
CaiI CAGNNNCTG 2 cut(s) 54, 203
Cfr13I GGNCC 3 cut(s) 60, 268, 320
ClaI ATCGAT 2 cut(s) 176, 230
Csp6I GTAC 2 cut(s) 277, 296
CviJI RGCY 4 cut(s) 10, 45, 269, 386
CviKI_1 RGCY 4 cut(s) 10, 45, 269, 386
CviQI GTAC 2 cut(s) 277, 296
DpnI GATC 4 cut(s) 38, 175, 182, 229
DpnII GATC 4 cut(s) 36, 173, 180, 227
Eam1104I CTCTTC 2 cut(s) 134, 258
EarI CTCTTC 2 cut(s) 134, 258
Eco47I GGWCC 2 cut(s) 60, 320
Eco88I CYCGRG 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 92, 107
FaiI YATR 2 cut(s) 86, 249
FauI CCCGC 1 cut(s) 297
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 46, 370
FokI GGATG 1 cut(s) 338
Fsp4HI GCNGC 2 cut(s) 46, 370
GluI GCNGC 2 cut(s) 46, 370
GsuI CTGGAG 1 cut(s) 91
HaeIII GGCC 1 cut(s) 269
HapII CCGG 3 cut(s) 58, 156, 323
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 1 cut(s) 22
HpaII CCGG 3 cut(s) 58, 156, 323
HphI GGTGA 2 cut(s) 31, 319
Hpy166II GTNNAC 1 cut(s) 145
Hpy188III TCNNGA 5 cut(s) 26, 133, 156, 323, 350
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 4 cut(s) 112, 130, 278, 304
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 3 cut(s) 48, 193, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 383
HpySE526I ACGT 1 cut(s) 147
Kpn2I TCCGGA 2 cut(s) 155, 322
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 4 cut(s) 36, 173, 180, 227
Lsp1109I GCAGC 2 cut(s) 32, 381
LweI GCATC 1 cut(s) 364
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 347
MalI GATC 4 cut(s) 38, 175, 182, 229
MbiI CCGCTC 1 cut(s) 304
MboI GATC 4 cut(s) 36, 173, 180, 227
MboII GAAGA 4 cut(s) 46, 121, 179, 275
MflI RGATCY 1 cut(s) 36
MluCI AATT 1 cut(s) 334
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 3 cut(s) 22, 73, 259
MroI TCCGGA 2 cut(s) 155, 322
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 3 cut(s) 58, 156, 323
MspR9I CCNGG 2 cut(s) 94, 109
MvaI CCWGG 2 cut(s) 94, 109
MwoI GCNNNNNNNGC 2 cut(s) 54, 383
NdeII GATC 4 cut(s) 36, 173, 180, 227
NlaIV GGNNCC 2 cut(s) 62, 321
NmuCI GTSAC 1 cut(s) 347
PaeR7I CTCGAG 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PkrI GCNGC 2 cut(s) 47, 371
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Psp6I CCWGG 2 cut(s) 92, 107
PspGI CCWGG 2 cut(s) 92, 107
PspN4I GGNNCC 2 cut(s) 62, 321
PspPI GGNCC 3 cut(s) 60, 268, 320
PstI CTGCAG 1 cut(s) 50
PstNI CAGNNNCTG 2 cut(s) 54, 203
PsuI RGATCY 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 2 cut(s) 278, 297
RsaNI GTAC 2 cut(s) 277, 296
SatI GCNGC 2 cut(s) 46, 370
Sau3AI GATC 4 cut(s) 36, 173, 180, 227
Sau96I GGNCC 3 cut(s) 60, 268, 320
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 2 cut(s) 94, 109
SetI ASST 6 cut(s) 47, 98, 141, 150, 315, 388
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 2 cut(s) 40, 46
Sfr274I CTCGAG 1 cut(s) 26
SinI GGWCC 2 cut(s) 60, 320
SlaI CTCGAG 1 cut(s) 26
SmlI CTYRAG 2 cut(s) 26, 299
SmoI CTYRAG 2 cut(s) 26, 299
Sse9I AATT 1 cut(s) 334
SsiI CCGC 1 cut(s) 304
StyD4I CCNGG 2 cut(s) 92, 107
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 150
TaqI TCGA 3 cut(s) 27, 176, 230
TasI AATT 1 cut(s) 334
TatI WGTACW 1 cut(s) 276
TscAI CASTG 2 cut(s) 59, 190
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 2 cut(s) 45, 369
Tsp45I GTSAC 1 cut(s) 347
TspGWI ACGGA 1 cut(s) 283
TspRI CASTG 2 cut(s) 59, 190
VpaK11BI GGWCC 2 cut(s) 60, 320
XhoI CTCGAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.