MD07G1220400.v1.1

Yippee-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004292
Physical Location & Seq
Reverse (-)
29775948 .. 29778963
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1220400.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGGGAGGCTGTTTGTGGTGAGTCTAGAGGGGAAGATCTACAGCTGCAAGCACTGCCGCACCCATCTTGCTCTTTGTGATGACGTAGTTTCCAAGTCTTTTTACTGCAGGCATGGGAAGGCTTATCTCTTCAGTAAGGTAGTGAATGTATATTCCGGGGAGTGTGAAGATAGAGCAATGATGACCGGACTGCACACAGTTGCTGACATTTTCTGCGTTGGGTGTGGATCGATAGTGGGGTGGAAATATGAGACTGCCCACGAAAAGGGCCAGAAGTACAAGGAAGGAAAATCCGTACTCGAGAGGATTAAGGTGTCGGGTCCGGAAGGAAGAACTTTTTGGGCCAGTCATGAAGCGCAAGTCGGTAGCAGCGATGCAGACGAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

130

Amino Acids

14.29

Weight (kDa)

7.61

Isoelectric Point (pI)

10.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 13 - 105 1.1e-12 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 1 cut(s) 58
AclWI GGATC 1 cut(s) 235
AcuI CTGAAG 1 cut(s) 115
AfaI GTAC 2 cut(s) 278, 297
AfiI CCNNNNNNNGG 1 cut(s) 265
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AluBI AGCT 1 cut(s) 45
AluI AGCT 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 235
AlwNI CAGNNNCTG 1 cut(s) 203
Ama87I CYCGRG 1 cut(s) 299
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 2 cut(s) 268, 342
ApeKI GCWGC 2 cut(s) 45, 369
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 3 cut(s) 268, 320, 342
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 320
BbvI GCAGC 2 cut(s) 32, 381
BccI CCATC 1 cut(s) 72
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 2 cut(s) 40, 106
BglII AGATCT 1 cut(s) 36
BisI GCNGC 3 cut(s) 46, 58, 370
BlsI GCNGC 3 cut(s) 47, 59, 371
Bme1390I CCNGG 1 cut(s) 157
Bme18I GGWCC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 3 cut(s) 268, 320, 342
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 364
BpuMI CCSGG 1 cut(s) 157
Bsa29I ATCGAT 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 156
BsaWI WCCGGW 2 cut(s) 185, 322
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 345
Bse3DI GCAATG 1 cut(s) 183
BseAI TCCGGA 1 cut(s) 322
BseCI ATCGAT 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 345
BseXI GCAGC 2 cut(s) 32, 381
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 2 cut(s) 270, 344
BshVI ATCGAT 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 156, 186, 323
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 245
BsnI GGCC 2 cut(s) 270, 344
BsoBI CYCGRG 1 cut(s) 299
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 2 cut(s) 36, 227
BspACI CCGC 1 cut(s) 58
BspANI GGCC 2 cut(s) 270, 344
BspDI ATCGAT 1 cut(s) 230
BspEI TCCGGA 1 cut(s) 322
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 321
BspMAI CTGCAG 1 cut(s) 110
BspPI GGATC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 2 cut(s) 36, 227
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 1 cut(s) 134
BstAPI GCANNNNNTGC 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 50, 110
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 2 cut(s) 39, 230
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 36, 227
BstMWI GCNNNNNNNGC 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 155
BstSFI CTRYAG 2 cut(s) 40, 106
BstV1I GCAGC 2 cut(s) 32, 381
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
Bsu15I ATCGAT 1 cut(s) 230
BsuRI GGCC 2 cut(s) 270, 344
BsuTUI ATCGAT 1 cut(s) 230
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 50, 110
CaiI CAGNNNCTG 1 cut(s) 203
CciI TCATGA 1 cut(s) 349
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 3 cut(s) 268, 320, 342
ClaI ATCGAT 1 cut(s) 230
Csp6I GTAC 2 cut(s) 277, 296
CviAII CATG 2 cut(s) 113, 350
CviJI RGCY 5 cut(s) 10, 45, 122, 270, 344
CviKI_1 RGCY 5 cut(s) 10, 45, 122, 270, 344
CviQI GTAC 2 cut(s) 277, 296
DpnI GATC 2 cut(s) 38, 229
DpnII GATC 2 cut(s) 36, 227
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 299
FaeI CATG 2 cut(s) 116, 353
FaiI YATR 4 cut(s) 114, 151, 249, 351
FatI CATG 2 cut(s) 112, 349
Fnu4HI GCNGC 3 cut(s) 46, 58, 370
Fsp4HI GCNGC 3 cut(s) 46, 58, 370
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 357
GluI GCNGC 3 cut(s) 46, 58, 370
HaeIII GGCC 2 cut(s) 270, 344
HapII CCGG 3 cut(s) 156, 186, 323
HhaI GCGC 1 cut(s) 358
Hin1II CATG 2 cut(s) 116, 353
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 1 cut(s) 22
HpaII CCGG 3 cut(s) 156, 186, 323
HphI GGTGA 1 cut(s) 31
Hpy188III TCNNGA 4 cut(s) 26, 301, 323, 350
HpyAV CCTTC 4 cut(s) 112, 278, 320, 377
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 4 cut(s) 48, 108, 193, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 54
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 2 cut(s) 116, 353
HspAI GCGC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 36, 227
LpnPI CCDG 6 cut(s) 94, 169, 199, 284, 336, 358
Lsp1109I GCAGC 2 cut(s) 32, 381
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 84
MalI GATC 2 cut(s) 38, 229
MboI GATC 2 cut(s) 36, 227
MboII GAAGA 4 cut(s) 46, 121, 179, 342
MflI RGATCY 1 cut(s) 36
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 2 cut(s) 22, 297
MroI TCCGGA 1 cut(s) 322
MseI TTAA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 3 cut(s) 156, 186, 323
MspR9I CCNGG 1 cut(s) 157
MwoI GCNNNNNNNGC 1 cut(s) 54
NciI CCSGG 1 cut(s) 157
NdeII GATC 2 cut(s) 36, 227
NlaIII CATG 2 cut(s) 116, 353
NlaIV GGNNCC 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 299
PagI TCATGA 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PkrI GCNGC 3 cut(s) 47, 59, 371
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 3 cut(s) 268, 320, 342
PstI CTGCAG 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 2 cut(s) 278, 297
RsaNI GTAC 2 cut(s) 277, 296
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 3 cut(s) 46, 58, 370
Sau3AI GATC 2 cut(s) 36, 227
Sau96I GGNCC 3 cut(s) 268, 320, 342
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 157
SetI ASST 5 cut(s) 47, 87, 141, 315, 388
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 2 cut(s) 40, 106
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 1 cut(s) 320
SlaI CTCGAG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SsiI CCGC 1 cut(s) 58
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 230, 300
TatI WGTACW 1 cut(s) 276
TauI GCSGC 1 cut(s) 60
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 59
TseI GCWGC 2 cut(s) 45, 369
TspDTI ATGAA 1 cut(s) 366
TspGWI ACGGA 1 cut(s) 283
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 320
XbaI TCTAGA 1 cut(s) 25
XhoI CTCGAG 1 cut(s) 299
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.