Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011056

Physical Location & Seq

Genomic Coordinates Reverse (-)

31235382 .. 31243106

Gene Structure 7725 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1220300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGATTATCAAGAAGAATCTCAAATCCCAAATGCCGAGTCTGAAAAGGTGCAAACTCGGCGAGTCACCGGGTGAGGACGACGATAACTCGGGCAGGAAGAAGAGGAAGACTAATGGGTATTATCCTCTCAGTCTGCTTGGCGAGGTCGCCGCCAGTATTATCCCCGTCAGCCTCCACGGCCTGCTCGGCTCGGTCGGGGCCTCCGAGCCGGGGTTCTCTGCTTCGTGGTGCACCAGAGTCTCCTGCTCCCCTGAGGATGAGTCGAAATCGAAGAACAGAGAGTCAGCCACCACAGCGAAGACGGAGGTTTCGCGGCCGCCGCTGGTGAGGACGTCGCGGGGCCGAGTTCAGGTACTTCCTTCTCGGTTTAATGATTCGGTAATTGAGAATTGGAAGAAAGAAAGTAAGACTAGTTTAAGAGATTATAATGTAGATGAGGAGATTGAATGTAAAAAAGAGAAACCTACCTTTAAAGCCCAGAAATTAGTTAGCCAAAATACGAAAAAGATGCGAAATGTGGATGGAATTGGATATAACAGTAAGAAGTATTCGGCTTTGTGTGAAGAAGATGAAGAGGAGGAAGTTGAGGGGAGTGTGCCATTTAAGAGTTTCGACAATAGGAAGTATTCGAGCTCACCAAGCACACTCTCGTCAATGCATGCGCAATTGGGTGAAGATGACAAGTGTGCAGTTGTGGAAATAGATGAAGGAAATGGTTTAGTGGAGACAGTGAGGGCTGCAAAGGAGAATGGTGAGAGGAAAGATGGCTTGTATGGACCCGAAGACTTCTATTCAGGCGATATAGTGTGGGCAATGCCAGGGAAAAAGGAGCCTTTCTGGCCTGCCATTGTCATTGATCCAATGTCGCAAGCGCCTGAGCTTGTTTTGAGGTCTTGCATACCCGATGCAGCTTGTGTGATGTTCTTTGGATACTCTGGGAATGGGAATCAACGGGATTATGCTTGGGTTGAACGTGGGAAGATTTTTCCCTTTATGGACTATGTGGACAGGTTCCAGGAGCAGCCTGAATTGAACAACTGCGAGCCCTGTGAATTCCAGTTGGCAATTGAGGAGGCATTTTTGGTGGAACAAGGGTTTACAGAAAAGTTGATAGCGGATATAAATATGGCAGCAATGTATGATGACTCTGTACGGAGAGGGGTTCAAGAGGCTACCGGTTCAAACCATGACCTGGACCAGGATGTATATGGGAAGAAGAAAGACATACGACCCTGTGAAGGCTGCGGCTTGTATTTTCCTTTTAAAATGATAAAGAAAATGAAGGATCCAAGTCCCAGAGGCCAATATCTTTGTAAATCGTGTGCTAGGTTAGTAAAATCGAAACATTATTGTGGGATATGCAAGAAGATTTGGAATGATTCAGACAGTGGAAGTTGGGTCCGATGTGATGGTTGTAAAGTGTGGGTGCATGCAGAATGTGACAAAATTTCCAGCAACGTTTTTAAGAACTTGGGAGCCACTGATTACTACTGCCCTACGTGCAAAGTCAAGTTTAATTTTGAATTATCAGATTCTGAAAGGGGGCAACCAAATGCAAAATGGAGTAAAAATAATGGTCAATTGGTGCTGCCTAACAAGGTTACTGTTCTCTGCAACTGCGTGGAAGGCATTTATTTTCCAAGTCTTCACTCTGTCGTGTGCAATTGTGGATTCTGTGGGGCAGAAAAGCAAGCACTTAGTGAATGGGAGCGACACACAGGTTCCAAATCGAGAAATTGGAGGACTAGTGTCAGAGTGAAAGGTTCCTTACTGCCACTGGAACAATGGATGTTGCAGTTAGCAGAGTATCATGAAAATGCTCTTGTGTCTTCTAAACCCCCTAAACGGCCTTCTATTAAAGAACGAAAGCAGAAGTTGCTTACTTTTTTGCAAGAAAAGTATGAACCTGTTCATGCCAAGTGGACAACAGAACGATGCGCTGTATGTAGATGGGTTGAAGATTGGGACTATAACAAAATCATCATCTGCAATAGATGTCAGATAGCTGTCCATCAAGAATGCTATGGAGCAAGAAATGTTCGGGATTTCACTTCGTGGGTCTGCAAAGCATGTGAAGCACCCAAAGTCAAGCAAGAATGTTGCCTCTGTCCTGTCAAAGGAGGTGCGTTAAAGCCAACTGATATTGACGGATTGTGGGTTCATATTACATGTGCTTGGTTCAGACCTGAAGTCTCTTTTGCAAGTGATGAAAAGATGGAGCCTGCTCTTGGCATCTTGAGTATTCCATCAAATTCTTTTGTGAAGATTTGCGTTATTTGTAAGCAAATTCATGGTTCATGCACGCAATGTTGCAAGTGTTCCACTTATTACCATGCAATGTGTGCCTCAAGGGCAGGTTATCGCATGGAGTTGCATTGTTTGGAGAAAAATGGAAAACAGACCACAAAAATGGTTTCTTATTGTGCTTACCACAGGGCTCCAAATCCAGATACGGTTTTAATCATACAGACTCCTCTAGGTGTGTTTTCAGCGAAAAGCCTTCTCCAAAATAAGAAGAAGCCTGGTTCAAGGTTAATTTCATCTAATAGAACAAAGCTTGAAGAGGTTTTGACAGTTGAAATGACAGAGCCTGAGTCTGAGCCCGAGCCATTATCTGCTGCAAGGTGCCGGGTGTACAATAGATTGAAAAATAACAAGAAGAGAGCAGAAGAAGAAACTGTTGCCCACCATGTGAAGGGACCTTCCCATCATCCCTTGGAAGCAATTCGAAGTTTGAACACATTCAGAGTAGTAGAGGATCCTCCAACTTTTTCGTCTTTTAGAGAACGGCTACACCATTTACAGAGAACAGAACACGATCGTGTTTGCTTTGGTAAATCTGGGATACATGGATGGGGTCTCTTTGCCCGTAGAGACATACAAGAAGGAGAAATGGTTCTTGAATATCGTGGTGAACAGGTGAGACGTAGTGTTGCAGATCTTAGGGAGACACGGTATAGATCAGAAGGCAAAGACTGTTATTTGTTTAAGATCAGTGAAGAAGTGGTTGTAGATGCCACTGATAAAGGAAATATAGCACGCCTGATCAACCATTCGTGCATGCCGAATTGCTATGCGAGGATCATGAGTGTGGGTGATGAAGAAAGCCGGATTGTACTTATTGCAAAGGCTGATGTAACTTGCGGCGATGAGCTAACGTACGATTACTTGTTCGATCCTAACGAGCCTGACGAATTCAAAGTCCCGTGTCTATGTAAAGCTCCCAACTGTCGGAAATTCATGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005911 GO:0008150 GO:0009292 GO:0009294 GO:0009506 GO:0009893 GO:0009987 GO:0010604 GO:0010638 GO:0019222 GO:0030054 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033043 GO:0033044 GO:0044764 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051569 GO:0051571 GO:0051704 GO:0055044 GO:0060255 GO:0065007 GO:0080090 GO:1902275 GO:1905269 GO:2001252

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD PHD_2 PWWP SET zf-HC5HC2H_2

Protein Analysis

1083

Amino Acids

122.61

Weight (kDa)

8.23

Isoelectric Point (pI)

46.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PWWP	PF00855	266 - 359	4.8e-12	PWWP domain
PHD	PF00628	451 - 501	6.3e-07	PHD-finger
PHD_2	PF13831	658 - 692	7.8e-12	PHD-finger
zf-HC5HC2H_2	PF13832	699 - 811	1.8e-26	PHD-zinc-finger like domain
zf-HC5HC2H	PF13771	721 - 812	4e-12	PHD-like zinc-binding domain
SET	PF00856	951 - 1056	1.8e-24	SET domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27910 AT5G53430
fragaria_vesca	FvH4_7g31710 FvH4_7g31710
malus_domestica	MD01G1220300.v1.1 MD07G1289800.v1.1
prunus_persica	Prupe.2G311000_v2.0.a1
pyrus_communis	pycom01g22990 pycom07g26250
rosa_chinensis	RchiOBHm_Chr1g0380901
rosa_laevigata	RLG00000026264
rosa_multiflora	Rmu_sc0002962.1_g000020
rosa_roxburghii	Rroxscaffold_4G00278980
rosa_rugosa	Rorug01G0425200 Rorug01G0425300
rosa_samantha	Rh1AG449100 Rh1BG404800 Rh1CG418800 Rh1DG435200
rosa_wichuraiana	Rw1G039150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	426
AatII	GACGTC	1 cut(s)	335
Acc16I	TGCGCA	1 cut(s)	663
Acc36I	ACCTGC	1 cut(s)	2345
AccB1I	GGYRCC	1 cut(s)	2625
AccB7I	CCANNNNNTGG	1 cut(s)	1192
AccII	CGCG	2 cut(s)	313, 337
AciI	CCGC	8 cut(s)	150, 313, 317, 320, 337, 1115, 1245, 3144
AclI	AACGTT	1 cut(s)	1458
AclWI	GGATC	7 cut(s)	851, 1280, 1293, 2753, 2766, 3089, 3170
AcoI	YGGCCR	1 cut(s)	314
AcsI	RAATTY	6 cut(s)	1052, 1446, 2251, 2286, 3194, 3236
AcuI	CTGAAG	1 cut(s)	2208
AcyI	GRCGYC	1 cut(s)	332
AdeI	CACNNNGTG	4 cut(s)	71, 1700, 2055, 2692
AfaI	GTAC	5 cut(s)	354, 1152, 2636, 3117, 3161
AflIII	ACRYGT	1 cut(s)	2168
AgeI	ACCGGT	1 cut(s)	1175
AhdI	GACNNNNNGTC	1 cut(s)	2189
AhlI	ACTAGT	2 cut(s)	410, 1745
AjnI	CCWGG	5 cut(s)	817, 1014, 1191, 1197, 2521
AleI	CACNNNNGTG	1 cut(s)	2820
AloI	GAACNNNNNNTCC	4 cut(s)	2142, 2174, 2880, 2912
AluBI	AGCT	7 cut(s)	633, 880, 911, 2006, 2557, 3154, 3221
AluI	AGCT	7 cut(s)	633, 880, 911, 2006, 2557, 3154, 3221
Alw21I	GWGCWC	2 cut(s)	233, 635
Alw26I	GTCTC	7 cut(s)	244, 719, 2197, 2864, 2868, 2917, 2942
Alw44I	GTGCAC	1 cut(s)	229
AlwI	GGATC	7 cut(s)	851, 1280, 1293, 2753, 2766, 3089, 3170
AlwNI	CAGNNNCTG	2 cut(s)	1535, 2591
Ama87I	CYCGRG	2 cut(s)	88, 2603
AoxI	GGCC	7 cut(s)	178, 198, 314, 340, 839, 1300, 1847
ApaLI	GTGCAC	1 cut(s)	229
ApeKI	GCWGC	7 cut(s)	737, 908, 1021, 1130, 1242, 1588, 2618
ApoI	RAATTY	6 cut(s)	1052, 1446, 2251, 2286, 3194, 3236
ArsI	GACNNNNNNTTYG	6 cut(s)	22, 54, 292, 324, 3186, 3218
AsiGI	ACCGGT	1 cut(s)	1175
Asp700I	GAANNNNTTC	4 cut(s)	1908, 2724, 2741, 2895
AspLEI	GCGC	3 cut(s)	664, 874, 1940
AspS9I	GGNCC	6 cut(s)	198, 340, 776, 1195, 1399, 2699
AsuC2I	CCSGG	3 cut(s)	69, 210, 2630
AsuHPI	GGTGA	9 cut(s)	57, 83, 337, 627, 683, 764, 2924, 2932, 3107
AsuII	TTCGAA	1 cut(s)	2728
AvaI	CYCGRG	2 cut(s)	88, 2603
AvaII	GGWCC	4 cut(s)	776, 1195, 1399, 2699
AxyI	CCTNAGG	1 cut(s)	252
BaeGI	GKGCMC	1 cut(s)	233
BamHI	GGATCC	2 cut(s)	1285, 2758
BanI	GGYRCC	1 cut(s)	2625
BanII	GRGCYC	4 cut(s)	635, 1047, 2440, 2604
BbsI	GAAGAC	5 cut(s)	113, 305, 789, 1637, 1821
Bbv12I	GWGCWC	2 cut(s)	233, 635
BbvI	GCAGC	7 cut(s)	724, 920, 1033, 1142, 1229, 1575, 2605
BccI	CCATC	9 cut(s)	515, 758, 1403, 1944, 2019, 2209, 2254, 2715, 2847
BceAI	ACGGC	3 cut(s)	193, 1862, 2804
BcgI	CGANNNNNNTGC	2 cut(s)	490, 524
BciT130I	CCWGG	5 cut(s)	819, 1016, 1193, 1199, 2523
BciVI	GTATCC	2 cut(s)	923, 2838
BclI	TGATCA	1 cut(s)	3045
BcnI	CCSGG	3 cut(s)	69, 210, 2630
BcoDI	GTCTC	7 cut(s)	244, 719, 2197, 2864, 2868, 2917, 2942
BcuI	ACTAGT	2 cut(s)	410, 1745
BfaI	CTAG	4 cut(s)	411, 1326, 1746, 2477
BfoI	RGCGCY	1 cut(s)	875
BfuAI	ACCTGC	1 cut(s)	2345
BfuI	GTATCC	2 cut(s)	923, 2838
BglI	GCCNNNNNGGC	4 cut(s)	177, 186, 838, 2351
BglII	AGATCT	1 cut(s)	2938
Bme1390I	CCNGG	8 cut(s)	69, 210, 819, 1016, 1193, 1199, 2523, 2630
Bme18I	GGWCC	4 cut(s)	776, 1195, 1399, 2699
BmeRI	GACNNNNNGTC	1 cut(s)	2189
BmeT110I	CYCGRG	2 cut(s)	88, 2603
BmgT120I	GGNCC	6 cut(s)	198, 340, 776, 1195, 1399, 2699
BmrFI	CCNGG	8 cut(s)	69, 210, 819, 1016, 1193, 1199, 2523, 2630
BmsI	GCATC	5 cut(s)	498, 895, 1925, 2241, 3004
BoxI	GACNNNNGTC	1 cut(s)	1748
BpiI	GAAGAC	5 cut(s)	113, 305, 789, 1637, 1821
Bpu10I	CCTNAGC	1 cut(s)	876
Bpu14I	TTCGAA	1 cut(s)	2728
BpuEI	CTTGAG	2 cut(s)	2257, 2332
BpuMI	CCSGG	3 cut(s)	69, 210, 2630
BsaAI	YACGTR	1 cut(s)	1500
BsaHI	GRCGYC	1 cut(s)	332
BsaI	GGTCTC	1 cut(s)	2864
BsaJI	CCNNGG	4 cut(s)	175, 209, 818, 2715
BsaWI	WCCGGW	1 cut(s)	1175
BsaXI	ACNNNNNCTCC	6 cut(s)	1732, 1762, 2880, 2910, 2939, 2969
Bse118I	RCCGGY	1 cut(s)	1175
Bse1I	ACTGG	3 cut(s)	153, 1057, 1782
Bse21I	CCTNAGG	1 cut(s)	252
Bse3DI	GCAATG	4 cut(s)	819, 1140, 2312, 2343
BseBI	CCWGG	5 cut(s)	819, 1016, 1193, 1199, 2523
BseDI	CCNNGG	4 cut(s)	175, 209, 818, 2715
BseGI	GGATG	6 cut(s)	262, 526, 1207, 1794, 2710, 2858
BseMI	GCAATG	4 cut(s)	819, 1140, 2312, 2343
BseMII	CTCAG	5 cut(s)	142, 243, 867, 2583, 2589
BseNI	ACTGG	3 cut(s)	153, 1057, 1782
BseRI	GAGGAG	4 cut(s)	452, 590, 1085, 2463
BseSI	GKGCMC	1 cut(s)	233
BseX3I	CGGCCG	1 cut(s)	314
BseXI	GCAGC	7 cut(s)	724, 920, 1033, 1142, 1229, 1575, 2605
BsgI	GTGCAG	1 cut(s)	708
Bsh1236I	CGCG	2 cut(s)	313, 337
Bsh1285I	CGRYCG	3 cut(s)	195, 317, 2821
BshFI	GGCC	7 cut(s)	180, 200, 316, 342, 841, 1302, 1849
BshNI	GGYRCC	1 cut(s)	2625
BshTI	ACCGGT	1 cut(s)	1175
BsiEI	CGRYCG	3 cut(s)	195, 317, 2821
BsiHKAI	GWGCWC	2 cut(s)	233, 635
BsiHKCI	CYCGRG	2 cut(s)	88, 2603
BsiSI	CCGG	5 cut(s)	68, 209, 1176, 2629, 3109
BsiWI	CGTACG	1 cut(s)	3159
BslFI	GGGAC	4 cut(s)	1278, 1979, 2712, 3188
BsmAI	GTCTC	7 cut(s)	244, 719, 2197, 2864, 2868, 2917, 2942
BsmBI	CGTCTC	1 cut(s)	2917
BsmFI	GGGAC	4 cut(s)	1278, 1979, 2712, 3188
BsmI	GAATGC	1 cut(s)	2024
BsnI	GGCC	7 cut(s)	180, 200, 316, 342, 841, 1302, 1849
Bso31I	GGTCTC	1 cut(s)	2864
BsoBI	CYCGRG	2 cut(s)	88, 2603
Bsp119I	TTCGAA	1 cut(s)	2728
Bsp1286I	GDGCHC	5 cut(s)	233, 635, 1047, 2440, 2604
Bsp1407I	TGTACA	1 cut(s)	2634
BspACI	CCGC	8 cut(s)	150, 313, 317, 320, 337, 1115, 1245, 3144
BspANI	GGCC	7 cut(s)	180, 200, 316, 342, 841, 1302, 1849
BspCNI	CTCAG	5 cut(s)	141, 244, 868, 2584, 2590
BspFNI	CGCG	2 cut(s)	313, 337
BspHI	TCATGA	3 cut(s)	1810, 3084, 3240
BspMI	ACCTGC	1 cut(s)	2345
BspPI	GGATC	7 cut(s)	851, 1280, 1293, 2753, 2766, 3089, 3170
BspT104I	TTCGAA	1 cut(s)	2728
BspT107I	GGYRCC	1 cut(s)	2625
BspTNI	GGTCTC	1 cut(s)	2864
BsrDI	GCAATG	4 cut(s)	819, 1140, 2312, 2343
BsrFI	RCCGGY	1 cut(s)	1175
BsrGI	TGTACA	1 cut(s)	2634
BsrI	ACTGG	3 cut(s)	153, 1057, 1782
BssAI	RCCGGY	1 cut(s)	1175
BssECI	CCNNGG	4 cut(s)	175, 209, 818, 2715
BssNI	GRCGYC	1 cut(s)	332
BssT1I	CCWWGG	1 cut(s)	2715
Bst2UI	CCWGG	5 cut(s)	819, 1016, 1193, 1199, 2523
Bst6I	CTCTTC	4 cut(s)	95, 567, 2556, 2654
BstACI	GRCGYC	1 cut(s)	332
BstAPI	GCANNNNNTGC	2 cut(s)	1876, 2342
BstAUI	TGTACA	1 cut(s)	2634
BstBAI	YACGTR	1 cut(s)	1500
BstBI	TTCGAA	1 cut(s)	2728
BstDEI	CTNAG	7 cut(s)	128, 252, 876, 1697, 2592, 2598, 2942
BstDSI	CCRYGG	1 cut(s)	175
BstENI	CCTNNNNNAGG	2 cut(s)	1196, 2115
BstF5I	GGATG	6 cut(s)	262, 526, 1207, 1794, 2710, 2858
BstFNI	CGCG	2 cut(s)	313, 337
BstH2I	RGCGCY	1 cut(s)	875
BstHHI	GCGC	3 cut(s)	664, 874, 1940
BstMAI	GTCTC	7 cut(s)	244, 719, 2197, 2864, 2868, 2917, 2942
BstMCI	CGRYCG	3 cut(s)	195, 317, 2821
BstNI	CCWGG	5 cut(s)	819, 1016, 1193, 1199, 2523
BstNSI	RCATGY	5 cut(s)	662, 1433, 2073, 2172, 3064
BstPAI	GACNNNNGTC	1 cut(s)	1748
BstSCI	CCNGG	8 cut(s)	67, 208, 817, 1014, 1191, 1197, 2521, 2628
BstSLI	GKGCMC	1 cut(s)	233
BstUI	CGCG	2 cut(s)	313, 337
BstV1I	GCAGC	7 cut(s)	724, 920, 1033, 1142, 1229, 1575, 2605
BstV2I	GAAGAC	5 cut(s)	113, 305, 789, 1637, 1821
BstX2I	RGATCY	3 cut(s)	1285, 2758, 2938
BstXI	CCANNNNNNTGG	1 cut(s)	2410
BstYI	RGATCY	3 cut(s)	1285, 2758, 2938
BstZI	CGGCCG	1 cut(s)	314
Bsu36I	CCTNAGG	1 cut(s)	252
BsuI	GTATCC	2 cut(s)	923, 2838
BsuRI	GGCC	7 cut(s)	180, 200, 316, 342, 841, 1302, 1849
BtgI	CCRYGG	1 cut(s)	175
BtgZI	GCGATG	1 cut(s)	3162
BtsCI	GGATG	6 cut(s)	262, 526, 1207, 1794, 2710, 2858
BtsIMutI	CAGTG	6 cut(s)	735, 1393, 1479, 1775, 3001, 3018
BveI	ACCTGC	1 cut(s)	2345
CaiI	CAGNNNCTG	2 cut(s)	1535, 2591
CciI	TCATGA	3 cut(s)	1810, 3084, 3240
CciNI	GCGGCCGC	1 cut(s)	314
CfoI	GCGC	3 cut(s)	664, 874, 1940
Cfr10I	RCCGGY	1 cut(s)	1175
Cfr13I	GGNCC	6 cut(s)	198, 340, 776, 1195, 1399, 2699
Csp6I	GTAC	5 cut(s)	353, 1151, 2635, 3116, 3160
CspAI	ACCGGT	1 cut(s)	1175
CviQI	GTAC	5 cut(s)	353, 1151, 2635, 3116, 3160
DdeI	CTNAG	7 cut(s)	128, 252, 876, 1697, 2592, 2598, 2942
DraI	TTTAAA	2 cut(s)	472, 1264
DraIII	CACNNNGTG	4 cut(s)	71, 1700, 2055, 2692
DriI	GACNNNNNGTC	1 cut(s)	2189
EaeI	YGGCCR	1 cut(s)	314
EagI	CGGCCG	1 cut(s)	314
Eam1104I	CTCTTC	4 cut(s)	95, 567, 2556, 2654
Eam1105I	GACNNNNNGTC	1 cut(s)	2189
EarI	CTCTTC	4 cut(s)	95, 567, 2556, 2654
Ecl136II	GAGCTC	1 cut(s)	633
EclXI	CGGCCG	1 cut(s)	314
Eco130I	CCWWGG	1 cut(s)	2715
Eco24I	GRGCYC	4 cut(s)	635, 1047, 2440, 2604
Eco31I	GGTCTC	1 cut(s)	2864
Eco47I	GGWCC	4 cut(s)	776, 1195, 1399, 2699
Eco52I	CGGCCG	1 cut(s)	314
Eco53kI	GAGCTC	1 cut(s)	633
Eco57I	CTGAAG	1 cut(s)	2208
Eco81I	CCTNAGG	1 cut(s)	252
Eco88I	CYCGRG	2 cut(s)	88, 2603
EcoICRI	GAGCTC	1 cut(s)	633
EcoNI	CCTNNNNNAGG	2 cut(s)	1196, 2115
EcoO109I	RGGNCCY	2 cut(s)	198, 2699
EcoRI	GAATTC	2 cut(s)	1052, 3194
EcoRII	CCWGG	5 cut(s)	817, 1014, 1191, 1197, 2521
EcoT14I	CCWWGG	1 cut(s)	2715
EcoT22I	ATGCAT	1 cut(s)	660
EcoT38I	GRGCYC	4 cut(s)	635, 1047, 2440, 2604
ErhI	CCWWGG	1 cut(s)	2715
Esp3I	CGTCTC	1 cut(s)	2917
FaqI	GGGAC	4 cut(s)	1278, 1979, 2712, 3188
FauI	CCCGC	1 cut(s)	330
FbaI	TGATCA	1 cut(s)	3045
FokI	GGATG	6 cut(s)	269, 533, 1214, 1801, 2697, 2865
FriOI	GRGCYC	4 cut(s)	635, 1047, 2440, 2604
FspBI	CTAG	4 cut(s)	411, 1326, 1746, 2477
FspI	TGCGCA	1 cut(s)	663
GlaI	GCGC	3 cut(s)	663, 873, 1939
HaeII	RGCGCY	1 cut(s)	875
HaeIII	GGCC	7 cut(s)	180, 200, 316, 342, 841, 1302, 1849
HapII	CCGG	5 cut(s)	68, 209, 1176, 2629, 3109
HhaI	GCGC	3 cut(s)	664, 874, 1940
Hin1I	GRCGYC	1 cut(s)	332
Hin6I	GCGC	3 cut(s)	662, 872, 1938
HinP1I	GCGC	3 cut(s)	662, 872, 1938
HindIII	AAGCTT	1 cut(s)	2555
HpaII	CCGG	5 cut(s)	68, 209, 1176, 2629, 3109
HphI	GGTGA	9 cut(s)	57, 83, 337, 627, 683, 764, 2924, 2932, 3107
Hpy166II	GTNNAC	6 cut(s)	231, 1006, 1098, 1923, 2635, 2915
Hpy8I	GTNNAC	6 cut(s)	231, 1006, 1098, 1923, 2635, 2915
Hpy99I	CGWCG	2 cut(s)	83, 337
HpyCH4IV	ACGT	6 cut(s)	332, 973, 1458, 1499, 2926, 3158
HpyF3I	CTNAG	7 cut(s)	128, 252, 876, 1697, 2592, 2598, 2942
HpySE526I	ACGT	6 cut(s)	332, 973, 1458, 1499, 2926, 3158
Hsp92I	GRCGYC	1 cut(s)	332
HspAI	GCGC	3 cut(s)	662, 872, 1938
Ksp22I	TGATCA	1 cut(s)	3045
LmnI	GCTCC	9 cut(s)	251, 829, 1018, 1475, 1708, 2027, 2218, 2443, 3226
Lsp1109I	GCAGC	7 cut(s)	724, 920, 1033, 1142, 1229, 1575, 2605
LweI	GCATC	5 cut(s)	498, 895, 1925, 2241, 3004
MaeI	CTAG	4 cut(s)	411, 1326, 1746, 2477
MaeII	ACGT	6 cut(s)	332, 973, 1458, 1499, 2926, 3158
MaeIII	GTNAC	4 cut(s)	63, 1439, 1600, 3136
MfeI	CAATTG	4 cut(s)	665, 1065, 1580, 1663
MflI	RGATCY	3 cut(s)	1285, 2758, 2938
MhlI	GDGCHC	5 cut(s)	233, 635, 1047, 2440, 2604
MlyI	GAGTC	8 cut(s)	46, 71, 246, 269, 290, 1139, 2464, 2603
MmeI	TCCRAC	2 cut(s)	2789, 3212
Mph1103I	ATGCAT	1 cut(s)	660
MroXI	GAANNNNTTC	4 cut(s)	1908, 2724, 2741, 2895
MslI	CAYNNNNRTG	5 cut(s)	1350, 1815, 2408, 2820, 3089
MspA1I	CMGCKG	1 cut(s)	322
MspI	CCGG	5 cut(s)	68, 209, 1176, 2629, 3109
MspR9I	CCNGG	8 cut(s)	69, 210, 819, 1016, 1193, 1199, 2523, 2630
MunI	CAATTG	4 cut(s)	665, 1065, 1580, 1663
Mva1269I	GAATGC	1 cut(s)	2024
MvaI	CCWGG	5 cut(s)	819, 1016, 1193, 1199, 2523
MvnI	CGCG	2 cut(s)	313, 337
NciI	CCSGG	3 cut(s)	69, 210, 2630
NmeAIII	GCCGAG	4 cut(s)	36, 60, 165, 368
NmuCI	GTSAC	2 cut(s)	63, 1439
NotI	GCGGCCGC	1 cut(s)	314
NsbI	TGCGCA	1 cut(s)	663
NsiI	ATGCAT	1 cut(s)	660
NspI	RCATGY	5 cut(s)	662, 1433, 2073, 2172, 3064
NspV	TTCGAA	1 cut(s)	2728
OliI	CACNNNNGTG	1 cut(s)	2820
PaeI	GCATGC	3 cut(s)	662, 1433, 3064
PagI	TCATGA	3 cut(s)	1810, 3084, 3240
PciI	ACATGT	1 cut(s)	2168
PcsI	WCGNNNNNNNCGW	4 cut(s)	201, 3062, 3180, 3189
PctI	GAATGC	1 cut(s)	2024
PdmI	GAANNNNTTC	4 cut(s)	1908, 2724, 2741, 2895
PfeI	GAWTC	6 cut(s)	16, 374, 946, 1379, 1532, 1671
Pfl23II	CGTACG	1 cut(s)	3159
PflMI	CCANNNNNTGG	1 cut(s)	1192
PfoI	TCCNGGA	1 cut(s)	1014
PinAI	ACCGGT	1 cut(s)	1175
Ple19I	CGATCG	1 cut(s)	2821
PleI	GAGTC	8 cut(s)	45, 70, 245, 268, 289, 1139, 2464, 2602
PpsI	GAGTC	8 cut(s)	45, 70, 245, 268, 289, 1139, 2464, 2602
Ppu21I	YACGTR	1 cut(s)	1500
PpuMI	RGGWCCY	1 cut(s)	2699
PscI	ACATGT	1 cut(s)	2168
PshAI	GACNNNNGTC	1 cut(s)	1748
PsiI	TTATAA	1 cut(s)	426
Psp124BI	GAGCTC	1 cut(s)	635
Psp1406I	AACGTT	1 cut(s)	1458
Psp5II	RGGWCCY	1 cut(s)	2699
Psp6I	CCWGG	5 cut(s)	817, 1014, 1191, 1197, 2521
PspGI	CCWGG	5 cut(s)	817, 1014, 1191, 1197, 2521
PspLI	CGTACG	1 cut(s)	3159
PspPI	GGNCC	6 cut(s)	198, 340, 776, 1195, 1399, 2699
PspPPI	RGGWCCY	1 cut(s)	2699
PstNI	CAGNNNCTG	2 cut(s)	1535, 2591
PsuI	RGATCY	3 cut(s)	1285, 2758, 2938
PvuI	CGATCG	1 cut(s)	2821
RsaI	GTAC	5 cut(s)	354, 1152, 2636, 3117, 3161
RsaNI	GTAC	5 cut(s)	353, 1151, 2635, 3116, 3160
RseI	CAYNNNNRTG	5 cut(s)	1350, 1815, 2408, 2820, 3089
SacI	GAGCTC	1 cut(s)	635
Sau96I	GGNCC	6 cut(s)	198, 340, 776, 1195, 1399, 2699
SchI	GAGTC	8 cut(s)	46, 71, 246, 269, 290, 1139, 2464, 2603
ScrFI	CCNGG	8 cut(s)	69, 210, 819, 1016, 1193, 1199, 2523, 2630
SduI	GDGCHC	5 cut(s)	233, 635, 1047, 2440, 2604
SfaNI	GCATC	5 cut(s)	498, 895, 1925, 2241, 3004
SfuI	TTCGAA	1 cut(s)	2728
SinI	GGWCC	4 cut(s)	776, 1195, 1399, 2699
SmiMI	CAYNNNNRTG	5 cut(s)	1350, 1815, 2408, 2820, 3089
SmlI	CTYRAG	2 cut(s)	2236, 2347
SmoI	CTYRAG	2 cut(s)	2236, 2347
SpeI	ACTAGT	2 cut(s)	410, 1745
SphI	GCATGC	3 cut(s)	662, 1433, 3064
SsiI	CCGC	8 cut(s)	150, 313, 317, 320, 337, 1115, 1245, 3144
SspMI	CTAG	4 cut(s)	411, 1326, 1746, 2477
SstI	GAGCTC	1 cut(s)	635
StyD4I	CCNGG	8 cut(s)	67, 208, 817, 1014, 1191, 1197, 2521, 2628
StyI	CCWWGG	1 cut(s)	2715
TaiI	ACGT	6 cut(s)	335, 976, 1461, 1502, 2929, 3161
TaqI	TCGA	8 cut(s)	263, 269, 612, 629, 1340, 1730, 2728, 3174
TaqII	GACCGA	1 cut(s)	181
TatI	WGTACW	2 cut(s)	2634, 3115
TauI	GCSGC	6 cut(s)	152, 316, 319, 322, 1248, 3147
TfiI	GAWTC	6 cut(s)	16, 374, 946, 1379, 1532, 1671
TscAI	CASTG	6 cut(s)	735, 1393, 1486, 1782, 3001, 3025
TseFI	GTSAC	2 cut(s)	63, 1439
TseI	GCWGC	7 cut(s)	737, 908, 1021, 1130, 1242, 1588, 2618
Tsp45I	GTSAC	2 cut(s)	63, 1439
TspGWI	ACGGA	3 cut(s)	317, 1168, 2163
TspRI	CASTG	6 cut(s)	735, 1393, 1486, 1782, 3001, 3025
Van91I	CCANNNNNTGG	1 cut(s)	1192
VneI	GTGCAC	1 cut(s)	229
VpaK11BI	GGWCC	4 cut(s)	776, 1195, 1399, 2699
XagI	CCTNNNNNAGG	2 cut(s)	1196, 2115
XapI	RAATTY	6 cut(s)	1052, 1446, 2251, 2286, 3194, 3236
XceI	RCATGY	5 cut(s)	662, 1433, 2073, 2172, 3064
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1205, 1557, 1782
XmnI	GAANNNNTTC	4 cut(s)	1908, 2724, 2741, 2895
XspI	CTAG	4 cut(s)	411, 1326, 1746, 2477
ZraI	GACGTC	1 cut(s)	333
Zsp2I	ATGCAT	1 cut(s)	660

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.