Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004642

Physical Location & Seq

Genomic Coordinates Reverse (-)

501128 .. 503369

Gene Structure 2242 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1008000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 483 bp

ATGTCGGACGAGGAGCACCAGTTCGAATCCAAGGCCGACGCCGGAGCATCCAAGACCTACCCCCAGCAGGCTGGTACCATCCGCAAGAATGGCTACATCGTCATCAAGGCCAGGCCTTGCAAGGTTGTTGAAGTCTCCACTTCCAAAACCGGAAAGCACGGTCACGCTAAGTGTCACTTTGTGGCAATTGATATTTTCAATGGAAAGAAGCTTGAAGATATTGTTCCTTCGTCCCACAATTGTGATGTTCCCCATGTCAACCGTACTGACTATCAGCTGATTGATATCTCTGAGGATGGCTTTGTGAGTCTCTTGACTGAAAATGGAAACACCAAGGATGATCTGAGGCTGCCCACTGATGACAGTCTGCTTACCCAGATCAAGGACGGGTTTAATGATGGAAAGGATCTTGTGGTGACTGTCATGTCTGCCATGGGAGAGGAGCAGATCTGTGCCCTTAAGGACATTGGTCCGAAGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

eIF-5a

Protein Analysis

161

Amino Acids

17.55

Weight (kDa)

5.46

Isoelectric Point (pI)

28.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IF5A-like_N	PF21485	17 - 79	1.3e-36	Translation initiation factor 5A-like, N-terminal
eIF-5a	PF01287	85 - 154	1.9e-29	Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004642)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13950 AT1G26630 AT1G26630 AT1G69410
fragaria_vesca	FvH4_1g00990 FvH4_4g30601
malus_domestica	MD02G1008000.v1.1 MD13G1067300.v1.1 MD15G1152300.v1.1 MD16G1066400.v1.1
prunus_persica	Prupe.1G280900_v2.0.a1 Prupe.7G262400_v2.0.a1
pyrus_communis	pycom02g00700 pycom13g05920 pycom15g13690 pycom16g05780
rosa_chinensis	RchiOBHm_Chr2g0085721 RchiOBHm_Chr4g0439011
rosa_laevigata	RLG00000006311 RLG00000015714
rosa_multiflora	Rmu_co8316413.1_g000001 Rmu_co8377829.1_g000001 Rmu_sc0009759.1_g000010 Rmu_sc0018732.1_g000002 Rmu_sc0023131.1_g000001
rosa_roxburghii	Rroxscaffold_5G00380020
rosa_rugosa	Rorug01G0462800 Rorug04G0315100
rosa_samantha	Rh4CG391000 Rh4DG371600
rosa_wichuraiana	Rw2G001050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	424
Acc65I	GGTACC	1 cut(s)	74
AccB1I	GGYRCC	1 cut(s)	74
AciI	CCGC	1 cut(s)	82
AclWI	GGATC	1 cut(s)	414
AcyI	GRCGYC	1 cut(s)	39
AdeI	CACNNNGTG	1 cut(s)	181
AfaI	GTAC	2 cut(s)	76, 265
AfiI	CCNNNNNNNGG	2 cut(s)	67, 382
AflII	CTTAAG	1 cut(s)	458
AgsI	TTSAA	3 cut(s)	131, 199, 215
AjnI	CCWGG	1 cut(s)	110
AleI	CACNNNNGTG	1 cut(s)	240
AloI	GAACNNNNNNTCC	1 cut(s)	37
AluBI	AGCT	2 cut(s)	211, 277
AluI	AGCT	2 cut(s)	211, 277
Alw21I	GWGCWC	1 cut(s)	18
Alw26I	GTCTC	2 cut(s)	139, 314
AlwI	GGATC	1 cut(s)	414
AoxI	GGCC	3 cut(s)	33, 108, 113
ApeKI	GCWGC	1 cut(s)	349
Asp718I	GGTACC	1 cut(s)	74
AspS9I	GGNCC	1 cut(s)	470
AsuHPI	GGTGA	1 cut(s)	427
AsuII	TTCGAA	1 cut(s)	24
AvaII	GGWCC	1 cut(s)	470
BaeGI	GKGCMC	1 cut(s)	457
BanI	GGYRCC	1 cut(s)	74
Bbv12I	GWGCWC	1 cut(s)	18
BbvI	GCAGC	1 cut(s)	336
BccI	CCATC	3 cut(s)	86, 290, 392
BciT130I	CCWGG	1 cut(s)	112
BcoDI	GTCTC	2 cut(s)	139, 314
BfrI	CTTAAG	1 cut(s)	458
BglII	AGATCT	1 cut(s)	447
BisI	GCNGC	1 cut(s)	350
BlsI	GCNGC	1 cut(s)	351
Bme1390I	CCNGG	1 cut(s)	112
Bme18I	GGWCC	1 cut(s)	470
BmgT120I	GGNCC	1 cut(s)	470
BmiI	GGNNCC	1 cut(s)	76
BmrFI	CCNGG	1 cut(s)	112
BmsI	GCATC	1 cut(s)	56
BoxI	GACNNNNGTC	1 cut(s)	468
Bpu14I	TTCGAA	1 cut(s)	24
BsaBI	GATNNNNATC	1 cut(s)	284
BsaHI	GRCGYC	1 cut(s)	39
BsaJI	CCNNGG	3 cut(s)	30, 333, 432
BsaWI	WCCGGW	1 cut(s)	149
BsaXI	ACNNNNNCTCC	2 cut(s)	5, 35
Bsc4I	CCNNNNNNNGG	2 cut(s)	67, 382
Bse1I	ACTGG	1 cut(s)	19
Bse8I	GATNNNNATC	1 cut(s)	284
BseBI	CCWGG	1 cut(s)	112
BseDI	CCNNGG	3 cut(s)	30, 333, 432
BseGI	GGATG	4 cut(s)	47, 78, 301, 343
BseJI	GATNNNNATC	1 cut(s)	284
BseLI	CCNNNNNNNGG	2 cut(s)	67, 382
BseMII	CTCAG	2 cut(s)	282, 335
BseNI	ACTGG	1 cut(s)	19
BseRI	GAGGAG	2 cut(s)	26, 455
BseSI	GKGCMC	1 cut(s)	457
BseXI	GCAGC	1 cut(s)	336
BseYI	CCCAGC	1 cut(s)	63
BshFI	GGCC	3 cut(s)	35, 110, 115
BshNI	GGYRCC	1 cut(s)	74
BsiHKAI	GWGCWC	1 cut(s)	18
BsiSI	CCGG	2 cut(s)	42, 150
BslFI	GGGAC	1 cut(s)	217
BslI	CCNNNNNNNGG	2 cut(s)	67, 382
BsmAI	GTCTC	2 cut(s)	139, 314
BsmFI	GGGAC	1 cut(s)	217
BsnI	GGCC	3 cut(s)	35, 110, 115
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	2 cut(s)	18, 457
Bsp143I	GATC	4 cut(s)	340, 378, 406, 447
Bsp19I	CCATGG	1 cut(s)	432
BspACI	CCGC	1 cut(s)	82
BspANI	GGCC	3 cut(s)	35, 110, 115
BspCNI	CTCAG	2 cut(s)	283, 336
BspLI	GGNNCC	1 cut(s)	76
BspPI	GGATC	1 cut(s)	414
BspT104I	TTCGAA	1 cut(s)	24
BspT107I	GGYRCC	1 cut(s)	74
BspTI	CTTAAG	1 cut(s)	458
BsrI	ACTGG	1 cut(s)	19
BssECI	CCNNGG	3 cut(s)	30, 333, 432
BssMI	GATC	4 cut(s)	340, 378, 406, 447
BssNI	GRCGYC	1 cut(s)	39
BssT1I	CCWWGG	3 cut(s)	30, 333, 432
Bst2UI	CCWGG	1 cut(s)	112
Bst4CI	ACNGT	4 cut(s)	161, 263, 365, 421
BstACI	GRCGYC	1 cut(s)	39
BstAFI	CTTAAG	1 cut(s)	458
BstBI	TTCGAA	1 cut(s)	24
BstC8I	GCNNGC	1 cut(s)	69
BstDEI	CTNAG	3 cut(s)	168, 291, 344
BstDSI	CCRYGG	1 cut(s)	432
BstF5I	GGATG	4 cut(s)	47, 78, 301, 343
BstKTI	GATC	4 cut(s)	343, 381, 409, 450
BstMAI	GTCTC	2 cut(s)	139, 314
BstMBI	GATC	4 cut(s)	340, 378, 406, 447
BstMWI	GCNNNNNNNGC	1 cut(s)	90
BstNI	CCWGG	1 cut(s)	112
BstPAI	GACNNNNGTC	1 cut(s)	468
BstSCI	CCNGG	1 cut(s)	110
BstSLI	GKGCMC	1 cut(s)	457
BstV1I	GCAGC	1 cut(s)	336
BstX2I	RGATCY	2 cut(s)	406, 447
BstXI	CCANNNNNNTGG	1 cut(s)	71
BstYI	RGATCY	2 cut(s)	406, 447
BsuRI	GGCC	3 cut(s)	35, 110, 115
BtgI	CCRYGG	1 cut(s)	432
BtsCI	GGATG	4 cut(s)	47, 78, 301, 343
BtsIMutI	CAGTG	1 cut(s)	354
Cac8I	GCNNGC	1 cut(s)	69
Cfr13I	GGNCC	1 cut(s)	470
CseI	GACGC	1 cut(s)	47
Csp6I	GTAC	2 cut(s)	75, 264
CviAII	CATG	3 cut(s)	254, 424, 433
CviJI	RGCY	9 cut(s)	35, 71, 93, 110, 115, 211, 277, 300, 349
CviKI_1	RGCY	9 cut(s)	35, 71, 93, 110, 115, 211, 277, 300, 349
CviQI	GTAC	2 cut(s)	75, 264
DdeI	CTNAG	3 cut(s)	168, 291, 344
DpnI	GATC	4 cut(s)	342, 380, 408, 449
DpnII	GATC	4 cut(s)	340, 378, 406, 447
DraIII	CACNNNGTG	1 cut(s)	181
DrdI	GACNNNNNNGTC	1 cut(s)	424
DseDI	GACNNNNNNGTC	1 cut(s)	424
Eco130I	CCWWGG	3 cut(s)	30, 333, 432
Eco147I	AGGCCT	1 cut(s)	115
Eco32I	GATATC	1 cut(s)	286
Eco47I	GGWCC	1 cut(s)	470
EcoRII	CCWGG	1 cut(s)	110
EcoRV	GATATC	1 cut(s)	286
EcoT14I	CCWWGG	3 cut(s)	30, 333, 432
ErhI	CCWWGG	3 cut(s)	30, 333, 432
FaeI	CATG	3 cut(s)	257, 427, 436
FaiI	YATR	3 cut(s)	255, 425, 434
FalI	AAGNNNNNCTT	2 cut(s)	161, 193
FaqI	GGGAC	1 cut(s)	217
FatI	CATG	3 cut(s)	253, 423, 432
Fnu4HI	GCNGC	1 cut(s)	350
FokI	GGATG	4 cut(s)	34, 65, 308, 350
Fsp4HI	GCNGC	1 cut(s)	350
GluI	GCNGC	1 cut(s)	350
GsaI	CCCAGC	1 cut(s)	67
HaeIII	GGCC	3 cut(s)	35, 110, 115
HapII	CCGG	2 cut(s)	42, 150
HgaI	GACGC	1 cut(s)	47
Hin1I	GRCGYC	1 cut(s)	39
Hin1II	CATG	3 cut(s)	257, 427, 436
HincII	GTYRAC	1 cut(s)	259
HindII	GTYRAC	1 cut(s)	259
HindIII	AAGCTT	1 cut(s)	209
HinfI	GANTC	2 cut(s)	26, 307
HpaII	CCGG	2 cut(s)	42, 150
HphI	GGTGA	1 cut(s)	427
Hpy166II	GTNNAC	1 cut(s)	259
Hpy188I	TCNGA	4 cut(s)	7, 292, 345, 474
Hpy188III	TCNNGA	1 cut(s)	313
Hpy8I	GTNNAC	1 cut(s)	259
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	1 cut(s)	237
HpyCH4III	ACNGT	4 cut(s)	161, 263, 365, 421
HpyCH4V	TGCA	1 cut(s)	120
HpyF10VI	GCNNNNNNNGC	1 cut(s)	90
HpyF3I	CTNAG	3 cut(s)	168, 291, 344
Hsp92I	GRCGYC	1 cut(s)	39
Hsp92II	CATG	3 cut(s)	257, 427, 436
KpnI	GGTACC	1 cut(s)	78
Kzo9I	GATC	4 cut(s)	340, 378, 406, 447
LmnI	GCTCC	3 cut(s)	13, 44, 442
LpnPI	CCDG	9 cut(s)	32, 53, 55, 57, 77, 97, 124, 163, 389
Lsp1109I	GCAGC	1 cut(s)	336
LweI	GCATC	1 cut(s)	56
MaeIII	GTNAC	3 cut(s)	161, 173, 415
MalI	GATC	4 cut(s)	342, 380, 408, 449
MboI	GATC	4 cut(s)	340, 378, 406, 447
MboII	GAAGA	1 cut(s)	227
MfeI	CAATTG	2 cut(s)	186, 238
MflI	RGATCY	2 cut(s)	406, 447
MhlI	GDGCHC	2 cut(s)	18, 457
MluCI	AATT	3 cut(s)	186, 238, 478
MlyI	GAGTC	1 cut(s)	316
MnlI	CCTC	4 cut(s)	4, 286, 339, 433
MseI	TTAA	3 cut(s)	393, 459, 481
MslI	CAYNNNNRTG	1 cut(s)	240
MspA1I	CMGCKG	1 cut(s)	277
MspCI	CTTAAG	1 cut(s)	458
MspI	CCGG	2 cut(s)	42, 150
MspR9I	CCNGG	1 cut(s)	112
MunI	CAATTG	2 cut(s)	186, 238
MvaI	CCWGG	1 cut(s)	112
MwoI	GCNNNNNNNGC	1 cut(s)	90
NcoI	CCATGG	1 cut(s)	432
NdeII	GATC	4 cut(s)	340, 378, 406, 447
NlaIII	CATG	3 cut(s)	257, 427, 436
NlaIV	GGNNCC	1 cut(s)	76
NmuCI	GTSAC	3 cut(s)	161, 173, 415
NspV	TTCGAA	1 cut(s)	24
OliI	CACNNNNGTG	1 cut(s)	240
PceI	AGGCCT	1 cut(s)	115
PfeI	GAWTC	1 cut(s)	26
PkrI	GCNGC	1 cut(s)	351
PleI	GAGTC	1 cut(s)	315
PpsI	GAGTC	1 cut(s)	315
PshAI	GACNNNNGTC	1 cut(s)	468
Psp6I	CCWGG	1 cut(s)	110
PspFI	CCCAGC	1 cut(s)	63
PspGI	CCWGG	1 cut(s)	110
PspN4I	GGNNCC	1 cut(s)	76
PspPI	GGNCC	1 cut(s)	470
PsuI	RGATCY	2 cut(s)	406, 447
PvuII	CAGCTG	1 cut(s)	277
RsaI	GTAC	2 cut(s)	76, 265
RsaNI	GTAC	2 cut(s)	75, 264
RseI	CAYNNNNRTG	1 cut(s)	240
SaqAI	TTAA	3 cut(s)	393, 459, 481
SatI	GCNGC	1 cut(s)	350
Sau3AI	GATC	4 cut(s)	340, 378, 406, 447
Sau96I	GGNCC	1 cut(s)	470
SchI	GAGTC	1 cut(s)	316
ScrFI	CCNGG	1 cut(s)	112
SduI	GDGCHC	2 cut(s)	18, 457
SetI	ASST	4 cut(s)	59, 126, 213, 279
SfaNI	GCATC	1 cut(s)	56
SfuI	TTCGAA	1 cut(s)	24
SinI	GGWCC	1 cut(s)	470
SmiMI	CAYNNNNRTG	1 cut(s)	240
SmlI	CTYRAG	1 cut(s)	458
SmoI	CTYRAG	1 cut(s)	458
Sse9I	AATT	3 cut(s)	186, 238, 478
SseBI	AGGCCT	1 cut(s)	115
SsiI	CCGC	1 cut(s)	82
StuI	AGGCCT	1 cut(s)	115
StyD4I	CCNGG	1 cut(s)	110
StyI	CCWWGG	3 cut(s)	30, 333, 432
TaaI	ACNGT	4 cut(s)	161, 263, 365, 421
TaqI	TCGA	1 cut(s)	24
TasI	AATT	3 cut(s)	186, 238, 478
TfiI	GAWTC	1 cut(s)	26
Tru1I	TTAA	3 cut(s)	393, 459, 481
Tru9I	TTAA	3 cut(s)	393, 459, 481
TscAI	CASTG	1 cut(s)	361
TseFI	GTSAC	3 cut(s)	161, 173, 415
TseI	GCWGC	1 cut(s)	349
Tsp45I	GTSAC	3 cut(s)	161, 173, 415
TspRI	CASTG	1 cut(s)	361
Vha464I	CTTAAG	1 cut(s)	458
VpaK11BI	GGWCC	1 cut(s)	470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.