Prupe.7G262400_v2.0.a1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0004642
Physical Location & Seq
Forward (+)
21789296 .. 21791818
2523 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G262400.1

Sequence Viewer

Length: 483 bp
ATGTCTGACGAGGAGCACCAGTTCGAATCCAAGGCCGATGCTGGGGCTTCCAAGACCTACCCTCAGCAGGCTGGTACCATTCGCAAGAATGGTTACATAGTCATCAAGGCCAGGCCTTGCAAGGTTGTTGAAGTCTCCACTTCAAAAACTGGCAAGCACGGTCATGCAAAGTGCCACTTTGTTGCAATTGATATTTTTAATGGAAAGAAACTTGAAGATATTGTTCCTTCATCCCACAACTGTGATGTTCCTCATGTCAACCGTACTGACTACCAGCTGATTGATATCTCTGAAGATGGTTTTGTGAGTCTTTTAACTGAAAATGGAAACACCAAGGATGATCTGAGGCTTCCCACCGATGAAAGTCTGCTTAGCCAGATTAAAGATGGGTTTGCTGATGGAAAGGATCTTGTGGTGACTGTCATGTCTGCAATGGGAGAGGAGCAGATCTGTGCTCTCAAGGATATTGGTCCTAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-5a

Protein Analysis

161

Amino Acids

17.5

Weight (kDa)

5.47

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 424
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 74
AclWI GGATC 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 2 cut(s) 76, 265
AfiI CCNNNNNNNGG 2 cut(s) 42, 67
AgsI TTSAA 3 cut(s) 131, 144, 215
AjnI CCWGG 1 cut(s) 110
AleI CACNNNNGTG 1 cut(s) 240
AloI GAACNNNNNNTCC 1 cut(s) 37
AluBI AGCT 1 cut(s) 277
AluI AGCT 1 cut(s) 277
Alw21I GWGCWC 2 cut(s) 18, 457
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 414
AoxI GGCC 3 cut(s) 33, 108, 113
Asp718I GGTACC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 427
AsuII TTCGAA 1 cut(s) 24
AvaII GGWCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 74
Bbv12I GWGCWC 2 cut(s) 18, 457
BbvCI CCTCAGC 1 cut(s) 63
BccI CCATC 3 cut(s) 290, 380, 392
BciT130I CCWGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 139
BglII AGATCT 1 cut(s) 447
BlpI GCTNAGC 1 cut(s) 371
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 371
Bpu14I TTCGAA 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 30, 333
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 67
Bse1I ACTGG 2 cut(s) 19, 154
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 284
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 30, 333
BseGI GGATG 2 cut(s) 230, 343
BseJI GATNNNNATC 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 42, 67
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 2 cut(s) 77, 335
BseNI ACTGG 2 cut(s) 19, 154
BseRI GAGGAG 2 cut(s) 26, 455
BseYI CCCAGC 1 cut(s) 41
BshFI GGCC 3 cut(s) 35, 110, 115
BshNI GGYRCC 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 18, 457
BslI CCNNNNNNNGG 2 cut(s) 42, 67
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 3 cut(s) 35, 110, 115
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 2 cut(s) 18, 457
Bsp143I GATC 3 cut(s) 340, 406, 447
Bsp1720I GCTNAGC 1 cut(s) 371
BspANI GGCC 3 cut(s) 35, 110, 115
BspCNI CTCAG 2 cut(s) 76, 336
BspLI GGNNCC 1 cut(s) 76
BspPI GGATC 1 cut(s) 414
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 2 cut(s) 19, 154
BssECI CCNNGG 2 cut(s) 30, 333
BssMI GATC 3 cut(s) 340, 406, 447
BssT1I CCWWGG 2 cut(s) 30, 333
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 4 cut(s) 161, 242, 263, 421
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 69, 155
BstDEI CTNAG 4 cut(s) 63, 344, 371, 474
BstF5I GGATG 2 cut(s) 230, 343
BstKTI GATC 3 cut(s) 343, 409, 450
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 3 cut(s) 340, 406, 447
BstNI CCWGG 1 cut(s) 112
BstSCI CCNGG 1 cut(s) 110
BstX2I RGATCY 2 cut(s) 406, 447
BstYI RGATCY 2 cut(s) 406, 447
BsuRI GGCC 3 cut(s) 35, 110, 115
BtsCI GGATG 2 cut(s) 230, 343
Cac8I GCNNGC 2 cut(s) 69, 155
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 75, 264
CviAII CATG 3 cut(s) 164, 254, 424
CviJI RGCY 8 cut(s) 35, 47, 71, 110, 115, 277, 349, 375
CviKI_1 RGCY 8 cut(s) 35, 47, 71, 110, 115, 277, 349, 375
CviQI GTAC 2 cut(s) 75, 264
DdeI CTNAG 4 cut(s) 63, 344, 371, 474
DpnI GATC 3 cut(s) 342, 408, 449
DpnII GATC 3 cut(s) 340, 406, 447
DrdI GACNNNNNNGTC 1 cut(s) 424
DseDI GACNNNNNNGTC 1 cut(s) 424
Eco130I CCWWGG 2 cut(s) 30, 333
Eco147I AGGCCT 1 cut(s) 115
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 110
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 30, 333
ErhI CCWWGG 2 cut(s) 30, 333
FaeI CATG 3 cut(s) 167, 257, 427
FaiI YATR 4 cut(s) 98, 165, 255, 425
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FatI CATG 3 cut(s) 163, 253, 423
FokI GGATG 2 cut(s) 217, 350
GsaI CCCAGC 1 cut(s) 45
HaeIII GGCC 3 cut(s) 35, 110, 115
Hin1II CATG 3 cut(s) 167, 257, 427
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 2 cut(s) 26, 307
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 3 cut(s) 7, 292, 345
Hpy8I GTNNAC 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 237
HpyCH4III ACNGT 4 cut(s) 161, 242, 263, 421
HpyCH4V TGCA 4 cut(s) 120, 167, 185, 431
HpyF3I CTNAG 4 cut(s) 63, 344, 371, 474
Hsp92II CATG 3 cut(s) 167, 257, 427
KpnI GGTACC 1 cut(s) 78
Kzo9I GATC 3 cut(s) 340, 406, 447
LmnI GCTCC 2 cut(s) 13, 442
LpnPI CCDG 9 cut(s) 27, 32, 53, 57, 97, 124, 135, 287, 389
LweI GCATC 1 cut(s) 28
MaeIII GTNAC 2 cut(s) 92, 415
MalI GATC 3 cut(s) 342, 408, 449
MboI GATC 3 cut(s) 340, 406, 447
MboII GAAGA 2 cut(s) 227, 305
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 2 cut(s) 406, 447
MhlI GDGCHC 2 cut(s) 18, 457
MluCI AATT 1 cut(s) 186
MlyI GAGTC 1 cut(s) 316
MnlI CCTC 5 cut(s) 4, 72, 261, 339, 433
MseI TTAA 3 cut(s) 198, 314, 381
MslI CAYNNNNRTG 2 cut(s) 162, 240
MspA1I CMGCKG 1 cut(s) 277
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 112
NdeII GATC 3 cut(s) 340, 406, 447
NlaIII CATG 3 cut(s) 167, 257, 427
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 415
NspV TTCGAA 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 240
PceI AGGCCT 1 cut(s) 115
PfeI GAWTC 1 cut(s) 26
PleI GAGTC 1 cut(s) 315
PpsI GAGTC 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 1 cut(s) 470
PsuI RGATCY 2 cut(s) 406, 447
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 2 cut(s) 76, 265
RsaNI GTAC 2 cut(s) 75, 264
RseI CAYNNNNRTG 2 cut(s) 162, 240
SaqAI TTAA 3 cut(s) 198, 314, 381
Sau3AI GATC 3 cut(s) 340, 406, 447
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 2 cut(s) 18, 457
SetI ASST 3 cut(s) 59, 126, 279
SfaNI GCATC 1 cut(s) 28
SfuI TTCGAA 1 cut(s) 24
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 2 cut(s) 162, 240
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 1 cut(s) 186
SseBI AGGCCT 1 cut(s) 115
StuI AGGCCT 1 cut(s) 115
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 2 cut(s) 30, 333
TaaI ACNGT 4 cut(s) 161, 242, 263, 421
TaqI TCGA 1 cut(s) 24
TasI AATT 1 cut(s) 186
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 3 cut(s) 198, 314, 381
Tru9I TTAA 3 cut(s) 198, 314, 381
TseFI GTSAC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 2 cut(s) 219, 375
VpaK11BI GGWCC 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.