Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004642

Physical Location & Seq

Genomic Coordinates Forward (+)

4634223 .. 4636535

Gene Structure 2313 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1066400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 480 bp

ATGTCGGACGAGGAGCACCACTTCGAATCCAAGGCCGACGCCGGAGCTTCCAAGACCTACCCTCAACAGGCCGGCACCATCCGCAAGAACGGTTACATTGTCATCAAAGCCAGGCCATGCAAGGTTGTGGAAGTTTCCACCTCCAAGACTGGAAAGCACGGTCATGCTAAGTGCCACTTTGTCGGTATCGACATCTTCACTGCCAAGAAGCTTGAGGATATTGTTCCATCTTCCCACAATTGTGATGTTCCACATGTCAACCGTACCGACTACCAGCTGATCGATATCTCTGAGGATGGATTTGTGAGTCTGCTGACTGAGAATGGTAACACCAAGGATGACCTGAGGCTTCCAACCGATGACAGTCTGCTTACGCAGATCAAGGATGGGTTTGCAGAGGGGAAGGATCTGGTTGTTTCTGTCATGTCTGCTATGGGAGAGGAGCAGATTTGCGCCCTCAAGGACATTGGTCCTAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko05166 map04110 map04111 map05166

Pfam Domains

Protein Families

eIF-5a

Protein Analysis

160

Amino Acids

17.39

Weight (kDa)

5.6

Isoelectric Point (pI)

28.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IF5A-like_N	PF21485	17 - 79	3.6e-37	Translation initiation factor 5A-like, N-terminal
eIF-5a	PF01287	85 - 154	2.8e-29	Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004642)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13950 AT1G26630 AT1G26630 AT1G69410
fragaria_vesca	FvH4_1g00990 FvH4_4g30601
malus_domestica	MD02G1008000.v1.1 MD13G1067300.v1.1 MD15G1152300.v1.1 MD16G1066400.v1.1
prunus_persica	Prupe.1G280900_v2.0.a1 Prupe.7G262400_v2.0.a1
pyrus_communis	pycom02g00700 pycom13g05920 pycom15g13690 pycom16g05780
rosa_chinensis	RchiOBHm_Chr2g0085721 RchiOBHm_Chr4g0439011
rosa_laevigata	RLG00000006311 RLG00000015714
rosa_multiflora	Rmu_co8316413.1_g000001 Rmu_co8377829.1_g000001 Rmu_sc0009759.1_g000010 Rmu_sc0018732.1_g000002 Rmu_sc0023131.1_g000001
rosa_roxburghii	Rroxscaffold_5G00380020
rosa_rugosa	Rorug01G0462800 Rorug04G0315100
rosa_samantha	Rh4CG391000 Rh4DG371600
rosa_wichuraiana	Rw2G001050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	74
AciI	CCGC	1 cut(s)	82
AclWI	GGATC	1 cut(s)	414
AcyI	GRCGYC	1 cut(s)	39
AfaI	GTAC	1 cut(s)	265
AfiI	CCNNNNNNNGG	1 cut(s)	67
AflIII	ACRYGT	1 cut(s)	253
AjnI	CCWGG	1 cut(s)	110
AleI	CACNNNNGTG	1 cut(s)	240
AluBI	AGCT	3 cut(s)	47, 211, 277
AluI	AGCT	3 cut(s)	47, 211, 277
Alw21I	GWGCWC	1 cut(s)	18
AlwI	GGATC	1 cut(s)	414
AoxI	GGCC	3 cut(s)	33, 69, 113
AspLEI	GCGC	1 cut(s)	455
AspS9I	GGNCC	1 cut(s)	470
AsuII	TTCGAA	1 cut(s)	24
AvaII	GGWCC	1 cut(s)	470
AxyI	CCTNAGG	1 cut(s)	344
BanI	GGYRCC	1 cut(s)	74
Bbv12I	GWGCWC	1 cut(s)	18
BccI	CCATC	4 cut(s)	86, 235, 290, 380
BciT130I	CCWGG	1 cut(s)	112
Bme1390I	CCNGG	1 cut(s)	112
Bme18I	GGWCC	1 cut(s)	470
BmgT120I	GGNCC	1 cut(s)	470
BmiI	GGNNCC	1 cut(s)	76
BmrFI	CCNGG	1 cut(s)	112
BoxI	GACNNNNGTC	1 cut(s)	468
Bpu14I	TTCGAA	1 cut(s)	24
BpuEI	CTTGAG	2 cut(s)	233, 443
Bsa29I	ATCGAT	1 cut(s)	282
BsaBI	GATNNNNATC	1 cut(s)	284
BsaHI	GRCGYC	1 cut(s)	39
BsaJI	CCNNGG	2 cut(s)	30, 333
Bsc4I	CCNNNNNNNGG	1 cut(s)	67
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	1 cut(s)	154
Bse21I	CCTNAGG	1 cut(s)	344
Bse8I	GATNNNNATC	1 cut(s)	284
BseBI	CCWGG	1 cut(s)	112
BseCI	ATCGAT	1 cut(s)	282
BseDI	CCNNGG	2 cut(s)	30, 333
BseGI	GGATG	4 cut(s)	78, 301, 343, 391
BseJI	GATNNNNATC	1 cut(s)	284
BseLI	CCNNNNNNNGG	1 cut(s)	67
BseMII	CTCAG	3 cut(s)	282, 309, 335
BseNI	ACTGG	1 cut(s)	154
BseRI	GAGGAG	2 cut(s)	26, 455
BshFI	GGCC	3 cut(s)	35, 71, 115
BshNI	GGYRCC	1 cut(s)	74
BshVI	ATCGAT	1 cut(s)	282
BsiHKAI	GWGCWC	1 cut(s)	18
BsiSI	CCGG	2 cut(s)	42, 72
BslI	CCNNNNNNNGG	1 cut(s)	67
BsnI	GGCC	3 cut(s)	35, 71, 115
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	1 cut(s)	18
Bsp143I	GATC	3 cut(s)	279, 378, 406
BspACI	CCGC	1 cut(s)	82
BspANI	GGCC	3 cut(s)	35, 71, 115
BspCNI	CTCAG	3 cut(s)	283, 310, 336
BspDI	ATCGAT	1 cut(s)	282
BspLI	GGNNCC	1 cut(s)	76
BspPI	GGATC	1 cut(s)	414
BspT104I	TTCGAA	1 cut(s)	24
BspT107I	GGYRCC	1 cut(s)	74
BsrFI	RCCGGY	1 cut(s)	71
BsrI	ACTGG	1 cut(s)	154
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	2 cut(s)	30, 333
BssMI	GATC	3 cut(s)	279, 378, 406
BssNI	GRCGYC	1 cut(s)	39
BssT1I	CCWWGG	2 cut(s)	30, 333
Bst2UI	CCWGG	1 cut(s)	112
Bst4CI	ACNGT	4 cut(s)	92, 161, 263, 365
BstACI	GRCGYC	1 cut(s)	39
BstBI	TTCGAA	1 cut(s)	24
BstC8I	GCNNGC	1 cut(s)	73
BstDEI	CTNAG	4 cut(s)	168, 291, 318, 344
BstF5I	GGATG	4 cut(s)	78, 301, 343, 391
BstHHI	GCGC	1 cut(s)	455
BstKTI	GATC	3 cut(s)	282, 381, 409
BstMBI	GATC	3 cut(s)	279, 378, 406
BstMWI	GCNNNNNNNGC	1 cut(s)	81
BstNI	CCWGG	1 cut(s)	112
BstNSI	RCATGY	1 cut(s)	257
BstPAI	GACNNNNGTC	1 cut(s)	468
BstSCI	CCNGG	1 cut(s)	110
BstX2I	RGATCY	1 cut(s)	406
BstYI	RGATCY	1 cut(s)	406
Bsu15I	ATCGAT	1 cut(s)	282
Bsu36I	CCTNAGG	1 cut(s)	344
BsuRI	GGCC	3 cut(s)	35, 71, 115
BsuTUI	ATCGAT	1 cut(s)	282
BtsCI	GGATG	4 cut(s)	78, 301, 343, 391
BtsI	GCAGTG	1 cut(s)	198
BtsIMutI	CAGTG	1 cut(s)	198
Cac8I	GCNNGC	1 cut(s)	73
CfoI	GCGC	1 cut(s)	455
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	1 cut(s)	470
ClaI	ATCGAT	1 cut(s)	282
CseI	GACGC	1 cut(s)	47
Csp6I	GTAC	1 cut(s)	264
CviAII	CATG	4 cut(s)	117, 164, 254, 424
CviJI	RGCY	8 cut(s)	35, 47, 71, 110, 115, 211, 277, 349
CviKI_1	RGCY	8 cut(s)	35, 47, 71, 110, 115, 211, 277, 349
CviQI	GTAC	1 cut(s)	264
DdeI	CTNAG	4 cut(s)	168, 291, 318, 344
DpnI	GATC	3 cut(s)	281, 380, 408
DpnII	GATC	3 cut(s)	279, 378, 406
Eco130I	CCWWGG	2 cut(s)	30, 333
Eco32I	GATATC	1 cut(s)	286
Eco47I	GGWCC	1 cut(s)	470
Eco81I	CCTNAGG	1 cut(s)	344
EcoRII	CCWGG	1 cut(s)	110
EcoRV	GATATC	1 cut(s)	286
EcoT14I	CCWWGG	2 cut(s)	30, 333
ErhI	CCWWGG	2 cut(s)	30, 333
FaeI	CATG	4 cut(s)	120, 167, 257, 427
FaiI	YATR	5 cut(s)	118, 165, 255, 425, 434
FalI	AAGNNNNNCTT	2 cut(s)	161, 193
FatI	CATG	4 cut(s)	116, 163, 253, 423
FokI	GGATG	4 cut(s)	65, 308, 350, 398
GlaI	GCGC	1 cut(s)	454
HaeIII	GGCC	3 cut(s)	35, 71, 115
HapII	CCGG	2 cut(s)	42, 72
HgaI	GACGC	1 cut(s)	47
HhaI	GCGC	1 cut(s)	455
Hin1I	GRCGYC	1 cut(s)	39
Hin1II	CATG	4 cut(s)	120, 167, 257, 427
Hin6I	GCGC	1 cut(s)	453
HinP1I	GCGC	1 cut(s)	453
HincII	GTYRAC	1 cut(s)	259
HindII	GTYRAC	1 cut(s)	259
HindIII	AAGCTT	1 cut(s)	209
HinfI	GANTC	2 cut(s)	26, 307
HpaII	CCGG	2 cut(s)	42, 72
Hpy166II	GTNNAC	1 cut(s)	259
Hpy188I	TCNGA	2 cut(s)	7, 292
Hpy8I	GTNNAC	1 cut(s)	259
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	1 cut(s)	397
HpyCH4III	ACNGT	4 cut(s)	92, 161, 263, 365
HpyCH4V	TGCA	2 cut(s)	120, 395
HpyF10VI	GCNNNNNNNGC	1 cut(s)	81
HpyF3I	CTNAG	4 cut(s)	168, 291, 318, 344
Hsp92I	GRCGYC	1 cut(s)	39
Hsp92II	CATG	4 cut(s)	120, 167, 257, 427
HspAI	GCGC	1 cut(s)	453
KroI	GCCGGC	1 cut(s)	71
KroNI	GCCGGC	1 cut(s)	73
Kzo9I	GATC	3 cut(s)	279, 378, 406
LmnI	GCTCC	3 cut(s)	13, 44, 442
LpnPI	CCDG	9 cut(s)	53, 55, 85, 97, 124, 135, 287, 356, 395
MaeIII	GTNAC	2 cut(s)	92, 326
MalI	GATC	3 cut(s)	281, 380, 408
MboI	GATC	3 cut(s)	279, 378, 406
MboII	GAAGA	2 cut(s)	187, 222
MfeI	CAATTG	1 cut(s)	238
MflI	RGATCY	1 cut(s)	406
MhlI	GDGCHC	1 cut(s)	18
MluCI	AATT	1 cut(s)	238
MlyI	GAGTC	1 cut(s)	316
MmeI	TCCRAC	1 cut(s)	377
MnlI	CCTC	9 cut(s)	4, 72, 151, 208, 286, 339, 391, 433, 467
MroNI	GCCGGC	1 cut(s)	71
MslI	CAYNNNNRTG	2 cut(s)	162, 240
MspA1I	CMGCKG	1 cut(s)	277
MspI	CCGG	2 cut(s)	42, 72
MspR9I	CCNGG	1 cut(s)	112
MunI	CAATTG	1 cut(s)	238
MvaI	CCWGG	1 cut(s)	112
MwoI	GCNNNNNNNGC	1 cut(s)	81
NaeI	GCCGGC	1 cut(s)	73
NdeII	GATC	3 cut(s)	279, 378, 406
NgoMIV	GCCGGC	1 cut(s)	71
NlaIII	CATG	4 cut(s)	120, 167, 257, 427
NlaIV	GGNNCC	1 cut(s)	76
NspI	RCATGY	1 cut(s)	257
NspV	TTCGAA	1 cut(s)	24
OliI	CACNNNNGTG	1 cut(s)	240
PciI	ACATGT	1 cut(s)	253
PdiI	GCCGGC	1 cut(s)	73
PfeI	GAWTC	1 cut(s)	26
PleI	GAGTC	1 cut(s)	315
PpsI	GAGTC	1 cut(s)	315
PscI	ACATGT	1 cut(s)	253
PshAI	GACNNNNGTC	1 cut(s)	468
Psp6I	CCWGG	1 cut(s)	110
PspGI	CCWGG	1 cut(s)	110
PspN4I	GGNNCC	1 cut(s)	76
PspPI	GGNCC	1 cut(s)	470
PsuI	RGATCY	1 cut(s)	406
PvuII	CAGCTG	1 cut(s)	277
RsaI	GTAC	1 cut(s)	265
RsaNI	GTAC	1 cut(s)	264
RseI	CAYNNNNRTG	2 cut(s)	162, 240
Sau3AI	GATC	3 cut(s)	279, 378, 406
Sau96I	GGNCC	1 cut(s)	470
SchI	GAGTC	1 cut(s)	316
ScrFI	CCNGG	1 cut(s)	112
SduI	GDGCHC	1 cut(s)	18
SetI	ASST	7 cut(s)	49, 59, 126, 143, 213, 279, 345
SfuI	TTCGAA	1 cut(s)	24
SinI	GGWCC	1 cut(s)	470
SmiMI	CAYNNNNRTG	2 cut(s)	162, 240
SmlI	CTYRAG	2 cut(s)	212, 458
SmoI	CTYRAG	2 cut(s)	212, 458
Sse9I	AATT	1 cut(s)	238
SsiI	CCGC	1 cut(s)	82
StyD4I	CCNGG	1 cut(s)	110
StyI	CCWWGG	2 cut(s)	30, 333
TaaI	ACNGT	4 cut(s)	92, 161, 263, 365
TaqI	TCGA	3 cut(s)	24, 189, 282
TasI	AATT	1 cut(s)	238
TfiI	GAWTC	1 cut(s)	26
TscAI	CASTG	1 cut(s)	205
TspRI	CASTG	1 cut(s)	205
VpaK11BI	GGWCC	1 cut(s)	470
XceI	RCATGY	1 cut(s)	257

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.