MD13G1067300.v1.1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004642
Physical Location & Seq
Forward (+)
4624633 .. 4626964
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1067300.v1.1.491

Sequence Viewer

Length: 480 bp
ATGTCGGACGAGGAGCATCACTTTGAATCGAAGGCCGACGCCGGAGCTTCCAAGACCTACCCTCAGCAGGCCGGTACTATCCGCAAGAACGGTTACATTGTCATCAAGGGCAGGCCATGCAAGGTTGTGGAAGTTTCCACCTCCAAGACTGGAAAGCACGGTCATGCCAAGTGCCACTTTGTCGGTATCGACATCTTCACTGCCAAGAAGCTTGAGGATATTGTTCCATCTTCCCACAATTGTGATGTTCCACATGTCAACCGTACTGACTACCAGCTGATCGATATCTCTGAGGATGGATTTGTGAGTCTGCTGACTGAGAATGGTAACACCAAGGACGACCTGAGACTTCCAACCGATGACAATCTGCTTACGCAGATCAAGGATGGGTTTGCAGAGGGGAAGGATCTGGTTGTTTCTGTCATGTCTGCTATGGGAGAGGAGCAGATTTGCGCCCTGAAGGACATCGGTCCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
eIF-5a

Protein Analysis

160

Amino Acids

17.4

Weight (kDa)

5.6

Isoelectric Point (pI)

27.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF5A-like_N PF21485 17 - 79 1e-37 Translation initiation factor 5A-like, N-terminal
eIF-5a PF01287 85 - 154 1.6e-29 Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 82
AclWI GGATC 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 76, 265
AfiI CCNNNNNNNGG 1 cut(s) 67
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 1 cut(s) 26
AleI CACNNNNGTG 1 cut(s) 240
AluBI AGCT 3 cut(s) 47, 211, 277
AluI AGCT 3 cut(s) 47, 211, 277
Alw26I GTCTC 1 cut(s) 340
AlwI GGATC 1 cut(s) 414
AoxI GGCC 3 cut(s) 33, 69, 113
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 1 cut(s) 470
AvaII GGWCC 1 cut(s) 470
BbvCI CCTCAGC 1 cut(s) 63
BccI CCATC 3 cut(s) 235, 290, 380
BcoDI GTCTC 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 472
BmsI GCATC 1 cut(s) 25
BoxI GACNNNNGTC 1 cut(s) 468
Bpu10I CCTNAGC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 233
Bsa29I ATCGAT 1 cut(s) 282
BsaBI GATNNNNATC 2 cut(s) 284, 363
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 333
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 154
Bse8I GATNNNNATC 2 cut(s) 284, 363
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 2 cut(s) 301, 391
BseJI GATNNNNATC 2 cut(s) 284, 363
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 4 cut(s) 77, 282, 309, 335
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 2 cut(s) 26, 455
BshFI GGCC 3 cut(s) 35, 71, 115
BshVI ATCGAT 1 cut(s) 282
BsiSI CCGG 2 cut(s) 42, 72
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 456
BsnI GGCC 3 cut(s) 35, 71, 115
Bsp143I GATC 3 cut(s) 279, 378, 406
BspACI CCGC 1 cut(s) 82
BspANI GGCC 3 cut(s) 35, 71, 115
BspCNI CTCAG 4 cut(s) 76, 283, 310, 336
BspDI ATCGAT 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 472
BspPI GGATC 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 3 cut(s) 279, 378, 406
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 333
Bst4CI ACNGT 3 cut(s) 92, 161, 263
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 69, 113
BstDEI CTNAG 4 cut(s) 63, 291, 318, 344
BstF5I GGATG 2 cut(s) 301, 391
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 3 cut(s) 282, 381, 409
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 3 cut(s) 279, 378, 406
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 257
BstPAI GACNNNNGTC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
Bsu15I ATCGAT 1 cut(s) 282
BsuRI GGCC 3 cut(s) 35, 71, 115
BsuTUI ATCGAT 1 cut(s) 282
BtsCI GGATG 2 cut(s) 301, 391
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 69, 113
CfoI GCGC 1 cut(s) 455
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 470
ClaI ATCGAT 1 cut(s) 282
CseI GACGC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 75, 264
CviAII CATG 4 cut(s) 117, 164, 254, 424
CviJI RGCY 6 cut(s) 35, 47, 71, 115, 211, 277
CviKI_1 RGCY 6 cut(s) 35, 47, 71, 115, 211, 277
CviQI GTAC 2 cut(s) 75, 264
DdeI CTNAG 4 cut(s) 63, 291, 318, 344
DpnI GATC 3 cut(s) 281, 380, 408
DpnII GATC 3 cut(s) 279, 378, 406
Eco130I CCWWGG 1 cut(s) 333
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 479
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 4 cut(s) 120, 167, 257, 427
FaiI YATR 5 cut(s) 118, 165, 255, 425, 434
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 456
FatI CATG 4 cut(s) 116, 163, 253, 423
FokI GGATG 2 cut(s) 308, 398
GlaI GCGC 1 cut(s) 454
HaeIII GGCC 3 cut(s) 35, 71, 115
HapII CCGG 2 cut(s) 42, 72
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 455
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 120, 167, 257, 427
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 2 cut(s) 26, 307
HpaII CCGG 2 cut(s) 42, 72
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 2 cut(s) 7, 292
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 25, 397, 454
HpyCH4III ACNGT 3 cut(s) 92, 161, 263
HpyCH4V TGCA 2 cut(s) 120, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 4 cut(s) 63, 291, 318, 344
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 120, 167, 257, 427
HspAI GCGC 1 cut(s) 453
Kzo9I GATC 3 cut(s) 279, 378, 406
LmnI GCTCC 3 cut(s) 13, 44, 442
LpnPI CCDG 9 cut(s) 53, 55, 85, 97, 135, 287, 356, 395, 470
LweI GCATC 1 cut(s) 25
MaeIII GTNAC 2 cut(s) 92, 326
MalI GATC 3 cut(s) 281, 380, 408
MboI GATC 3 cut(s) 279, 378, 406
MboII GAAGA 2 cut(s) 187, 222
MfeI CAATTG 1 cut(s) 238
MflI RGATCY 1 cut(s) 406
MluCI AATT 1 cut(s) 238
MlyI GAGTC 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 7 cut(s) 4, 72, 151, 208, 286, 391, 433
MslI CAYNNNNRTG 2 cut(s) 162, 240
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 2 cut(s) 42, 72
MunI CAATTG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 3 cut(s) 279, 378, 406
NlaIII CATG 4 cut(s) 120, 167, 257, 427
NlaIV GGNNCC 1 cut(s) 472
NspI RCATGY 1 cut(s) 257
OliI CACNNNNGTG 1 cut(s) 240
PciI ACATGT 1 cut(s) 253
PfeI GAWTC 1 cut(s) 26
PleI GAGTC 1 cut(s) 315
PpsI GAGTC 1 cut(s) 315
PscI ACATGT 1 cut(s) 253
PshAI GACNNNNGTC 1 cut(s) 468
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 1 cut(s) 470
PsuI RGATCY 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 2 cut(s) 76, 265
RsaNI GTAC 2 cut(s) 75, 264
RseI CAYNNNNRTG 2 cut(s) 162, 240
Sau3AI GATC 3 cut(s) 279, 378, 406
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 1 cut(s) 316
SetI ASST 7 cut(s) 49, 59, 126, 143, 213, 279, 345
SfaNI GCATC 1 cut(s) 25
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 2 cut(s) 162, 240
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 1 cut(s) 238
SsiI CCGC 1 cut(s) 82
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 3 cut(s) 92, 161, 263
TaqI TCGA 3 cut(s) 29, 189, 282
TaqII GACCGA 1 cut(s) 458
TasI AATT 1 cut(s) 238
TfiI GAWTC 1 cut(s) 26
TscAI CASTG 1 cut(s) 205
TspRI CASTG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 470
XceI RCATGY 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.