Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001857

Physical Location & Seq

Genomic Coordinates Forward (+)

2123553 .. 2140509

Gene Structure 16957 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1027700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3393 bp

ATGTCCTCGCTTAGCAGAGAGCTCGTTTTTCTCATACTTCAGTTTTTGGATGAAGAGAAATTCAAAGACAGTGTTCACAAGTTGGAGCAAGAGTCAGGGTTTTTCTTTAACATGAGGTACTTTGAGGACATGGTGACAAATGGAGAGTGGGAGGAGGTGGAAAAGTATTTGTCTGGTTTCACGAAGGTTGATGATAATCGATACTCTATGAAGATTTTCTTTGAGATTCGCAAGCAGAAGTACCTTGAAGCTTTAGACAAGCGGGATCGAGCAAAAGCTGTGGACATTTTAGTTAAGGACTTGAAAGTTTTTGCAGCATTTAATGAGGAACTTTTTAAGGAAATTACACAGTTGTTGACTTTGGAAAACTTTAGGGATAATGAACAGTTATCTAAATATGGTGATACTAAGTCTGCAAGGAGTATAATGCTTGCTGAACTGAAAAAGTTAATAGAGGCTAACCCATTGTTTCGTGATAAACTTCAATTTCCTACCCTGAAGAACTCGAGACTGCGTACTCTTATTAATCAGAGTTTAAACTGGCAGCATCAGCTTTGTAAGAACCCAAGGTCTAATCCAGACATAAAGACCCTGTTTGTAGACCACAGTTGTGGACAACCAAATGGTACCAGGGCTCCATCTCCTGTCACTAATCACTTAATGGGGGCAGTCCCTAAGACAGGGGGTTTCCCTCCATTGGGTGCTCATGGGCCCTTTCAGCCCACACCAGCTGCTCTTCCAACATCTCTTGCTGGATGGATGGCAAATCCATCACCTGTACCTCATCCCTCAGCTTCTGCTGGGCCTCTTGGGTTGGCCGCAGCTAACACTGCAGCCATCTTGAAGCGCCCTAGAACTCCTCCTACCGGTAACCCAACTATGGACTATCAAACAGCAGATTCTGAGCACATTCTAAAGAGATCAAGACCTTTCGGGATAACGGATGAAGCCAATAATCTGCCAGTAAATATGCTGCCTGTTGCATATCCTAATCAGAGCCATGGTCAGAGTTCCTACTCTTCTGATGACTTGCCCAGGTCTGTTGTTATGACTTTAAGTCCAGGTTCAACTGTCAAGAGTATGGATTTCCACCCGGTGCAACAAATTTTACTTCTTGTTGGAACAAACACGGGCGATGTCATGATATATAAGCTACCAAGCCAAGAAAAGATTGATATTAAGAACTTCAAAGTCTGGGACCTTGGAGCATGTTCAGGGGCACTGCAGGCATCTTTGGCCAGTGATTATACAGCATCAATCAACCGTGTGATGTGGAGCCCTGATGGGACACTTTTTGGTGTTGCATACTCTAAGCACATTGTACACATATATTCCTACCATGGTGTTGATGAGTTACGAAATCACCTGGAGATCGATGCCCATGTTGGCAGCGTTAATGATCTTGCTTTCTCATATCCAAACAAACAGCTGTGTGTTGTCACTTGCGGGGATGATAGACTCATTAAGGTGTGGGATGCAACCACTGGGAATAAGCAGTATACTTTTGAGGGCCATGAAGCACCTGTATATTCAGTGTGTCCACATCACAAAGAAAATATCCAGTTCATTTTCTCGACGGCTACTGATGGGAAAATAAAGGCATGGTTGTATGATAATGTGGGCTCAAGAGTTGACTATGATGCACCAGGCCATTCATCTACCACTATGGCATACAGTGCAGATGGAGCAAGGTTGTTCTCGTGTGGGACTAATAAAGAAGGGGATTCATACTTGGTGGAGTGGAATGAAAGTGAAGGAGCTGTAAAGCGGACCTATCATGGTCTTGCAAAGCGTACTGTTGGGGTTGTGCAGTTTGATACCACTAAAAACCGGTTCTTGGCTGCTGGCGATGAGTTCACTGTCAAATTTTGGGACATGGACAATGTTAACCTTTTGACGAGTACTGATGCAGATGGTGGATTGCCGGCTTCTCCCACAATCAGATTTAACAAGGAAGGAATATTGTTAGCTGCCTCAACGAACGATGGTATTAAAATTCTGGCAAATACAGATGGACTTAGGTTGCTAAGAACTGTGGAAAGTCGCACATTTGATGCTTCAAGAGCTGCTTCTGCGGCTGCAGTGAAGGCACCCCCAGTAGGAACATTTGGGCCTTCCAGCACTCCTATTGGAACAAGCATTGGAGATCGAGCTGCTCCTGTGTCAGCCATGGTTGGACTGAACAATGACAATAGAAGTTTGGTCGACATTAAACCTAGAATTGCTGATGAGTCAGTAGAGAAAGCCAGGATTTGGAAACTGACTGAAATCAGCGAACTATCACAATGCCGCTCTCTGAGGCTCCCTGATACTTTAACAGCAATGAGGGTTTCTAGATTGATATATACAAATTCAGGACTTGCAGTGTTGGCGTTATCATCTAATGCTGTACACAAGCTCTGGAAATGGCAAAGAAATGAACGAATACAAAAGGCCACAGCCAGTAGTCTACCGCAATTATGGCAACCTTCAAGTGGAATATTGATGACTAATGATATAAGTGATACAAACCCTGAGGATGCTGTTCCATGCTTTGCACTGTCAAAGAACGACTCTTATGTTATGTCAGCTTCAGGGGGGAAAATATCCCTTTTCAATATGATGACATTTAAGACAATGACAACATTTATGCCCCCACCCCCAGCTGCAACATATCTGGCATTTCATCCTCAAGACAATAATATCATTGCCATAGGAATGGATGATTCTTCTATCCAGATTTACAATGTACGGGTTGACGAGGTTAAAACCAAGCTAAAAGGTCATCAGAAAAGAATAACAGGCCTTGCCTTCTCTCATACTCTTAATGTGCTTGTATCTTCGGGGGCTGATTCCCAGTTATGTGTTTGGAACACGGATGGATGGGAGAAGCAGGCTAGTAAATTTCTTCAGATGCCTAGTGGCCGGGCTGCTGCTCCCCTTTCTGATACCCGAGTTCAGTTTCACTCAGATCAGACACATTTGCTGGCAGTTCATGAAACACAGATAGCCATATATGAAGCACCAAAATTGGAATGCCTTAAGCAGTGGGTTCCTCGAGAAGTTAGTGGTCCGATCACACATGCTGTATATTCCTGTGATAGCCAGTTAATTTATGTTAGCTTTGAAGATGGAAGTGTTGGGGTTCTCACTGCTCTTACACTTAGATTGAGATGCCGAATACTACCAACCGCTTATTTACCTCCAAATCCAAGCTTAAGGGTGTATCCTCTCGTTGTTGCGGCACATCCATCCGAACCCAACCAGTTTGCCTTAGGACTTTCAGACGGTGGAGTGCATGTACTTGAGCCATTAGAGTCAGAAGGAAAGTGGGGAACTAATCCCCCAATCGAAAATGGTGCTGGGCCTAGCGCGACATCTGGAGCAGCCGCTTCAGATCAACCCCAAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1131

Amino Acids

124.35

Weight (kDa)

6.58

Isoelectric Point (pI)

40.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	1.7e-12	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.5e-63	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	3.6e-24	TOPLESS, zinc finger domain
Beta-prop_RIG_2nd	PF23775	360 - 511	3e-07	RIG second beta-propeller
Beta-prop_WDR3_1st	PF25173	395 - 535	1.2e-14	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	414 - 571	3.3e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	416 - 628	2.4e-20	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	418 - 672	7.4e-22	THOC3 beta-propeller domain
WDR55	PF24796	418 - 535	1.1e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	419 - 572	6.2e-10	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	423 - 626	7.5e-06	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	434 - 538	2.1e-10	WDHD1 first WD40 domain
WD40_Prp19	PF24814	477 - 662	6.2e-10	Prp19 WD40 domain
WD40_Prp19	PF24814	790 - 1034	3.4e-16	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	792 - 956	2.1e-14	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	817 - 1050	4.7e-12	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	836 - 958	1.3e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	837 - 1042	5.9e-17	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	837 - 953	4.8e-16	WDR3 first beta-propeller domain
WDR55	PF24796	838 - 952	5.9e-07	WDR55
Beta-prop_WDR5	PF25175	840 - 1040	3e-18	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	841 - 952	9.1e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	843 - 960	4.7e-12	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	854 - 951	2.8e-10	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	855 - 958	2.3e-09	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	860 - 963	2.8e-08	WDR90/POC16, second beta-propeller
WD40	PF00400	916 - 951	9.5e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15880 AT3G15880 AT3G15880 AT3G15880
fragaria_vesca	FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730
malus_domestica	MD02G1027700.v1.1 MD11G1043800.v1.1 MD15G1169300.v1.1
prunus_persica	Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1
pyrus_communis	pycom12419g00020
rosa_chinensis	RchiOBHm_Chr2g0087841
rosa_laevigata	RLG00000015906 RLG00000019456
rosa_multiflora	Rmu_sc0008385.1_g000016
rosa_roxburghii	Rroxscaffold_2G00153180
rosa_rugosa	Rorug01G0479000 Rorug01G0479000 Rorug02G0324100 Rorug02G0324100
rosa_samantha	Rh2BG032700
rosa_wichuraiana	Rw2G002610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	626
AccB1I	GGYRCC	2 cut(s)	626, 2089
AccB7I	CCANNNNNTGG	1 cut(s)	2253
AccBSI	CCGCTC	1 cut(s)	2292
AccI	GTMKAC	4 cut(s)	600, 1499, 2205, 2449
AccII	CGCG	1 cut(s)	3353
AclWI	GGATC	1 cut(s)	273
AcoI	YGGCCR	3 cut(s)	816, 1236, 2902
AcsI	RAATTY	6 cut(s)	59, 1104, 1865, 1995, 2350, 2882
AcuI	CTGAAG	5 cut(s)	23, 518, 2556, 2873, 3357
AdeI	CACNNNGTG	1 cut(s)	1096
AfiI	CCNNNNNNNGG	9 cut(s)	680, 697, 698, 866, 880, 1837, 2099, 2253, 2474
AflII	CTTAAG	2 cut(s)	3020, 3196
AgeI	ACCGGT	2 cut(s)	866, 1830
AhdI	GACNNNNNGTC	1 cut(s)	1056
AjnI	CCWGG	6 cut(s)	629, 1034, 1060, 1365, 1645, 2246
AleI	CACNNNNGTG	1 cut(s)	609
Alw21I	GWGCWC	3 cut(s)	24, 706, 909
Alw26I	GTCTC	1 cut(s)	502
AlwI	GGATC	1 cut(s)	273
AlwNI	CAGNNNCTG	2 cut(s)	797, 902
Ama87I	CYCGRG	3 cut(s)	505, 2931, 3036
ApaI	GGGCCC	1 cut(s)	714
ApoI	RAATTY	6 cut(s)	59, 1104, 1865, 1995, 2350, 2882
AseI	ATTAAT	1 cut(s)	525
AsiGI	ACCGGT	2 cut(s)	866, 1830
Asp700I	GAANNNNTTC	1 cut(s)	215
Asp718I	GGTACC	1 cut(s)	626
AspLEI	GCGC	2 cut(s)	849, 3353
AspS9I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2111, 3050, 3344
AsuC2I	CCSGG	2 cut(s)	1094, 2906
AsuHPI	GGTGA	4 cut(s)	145, 413, 765, 1355
AvaI	CYCGRG	3 cut(s)	505, 2931, 3036
AvaII	GGWCC	3 cut(s)	1198, 1770, 3050
AxyI	CCTNAGG	2 cut(s)	2514, 3253
BaeGI	GKGCMC	2 cut(s)	714, 1222
BaeI	ACNNNNGTAYC	2 cut(s)	2919, 2952
BalI	TGGCCA	1 cut(s)	1238
BanI	GGYRCC	2 cut(s)	626, 2089
BanII	GRGCYC	5 cut(s)	24, 637, 714, 1280, 1625
BauI	CACGAG	1 cut(s)	1699
Bbv12I	GWGCWC	3 cut(s)	24, 706, 909
BbvCI	CCTCAGC	1 cut(s)	790
BceAI	ACGGC	1 cut(s)	1593
BcgI	CGANNNNNNTGC	4 cut(s)	4, 38, 3320, 3354
BciT130I	CCWGG	6 cut(s)	631, 1036, 1062, 1367, 1647, 2248
BciVI	GTATCC	1 cut(s)	3216
BcnI	CCSGG	2 cut(s)	1094, 2906
BcoDI	GTCTC	1 cut(s)	502
BfaI	CTAG	6 cut(s)	852, 2217, 2334, 2877, 2898, 3348
BfmI	CTRYAG	3 cut(s)	831, 1223, 2079
BfoI	RGCGCY	1 cut(s)	850
BfrI	CTTAAG	2 cut(s)	3020, 3196
BfuI	GTATCC	1 cut(s)	3216
BlpI	GCTNAGC	1 cut(s)	11
BmcAI	AGTACT	1 cut(s)	1903
Bme1390I	CCNGG	8 cut(s)	631, 1036, 1062, 1094, 1367, 1647, 2248, 2906
Bme18I	GGWCC	3 cut(s)	1198, 1770, 3050
BmeRI	GACNNNNNGTC	1 cut(s)	1056
BmeT110I	CYCGRG	3 cut(s)	505, 2931, 3036
BmgT120I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2111, 3050, 3344
BmiI	GGNNCC	8 cut(s)	628, 636, 712, 1199, 1277, 2091, 2303, 3033
BmrFI	CCNGG	8 cut(s)	631, 1036, 1062, 1094, 1367, 1647, 2248, 2906
BmrI	ACTGGG	3 cut(s)	1494, 2090, 2830
BmuI	ACTGGG	3 cut(s)	1494, 2090, 2830
BpmI	CTGGAG	2 cut(s)	1388, 3381
Bpu10I	CCTNAGC	1 cut(s)	790
Bpu1102I	GCTNAGC	1 cut(s)	11
BpuEI	CTTGAG	3 cut(s)	1609, 2655, 3305
BpuMI	CCSGG	2 cut(s)	1094, 2906
Bsa29I	ATCGAT	2 cut(s)	199, 1374
BsaBI	GATNNNNATC	1 cut(s)	195
BsaJI	CCNNGG	7 cut(s)	566, 630, 1000, 1034, 1201, 1339, 2169
BsaWI	WCCGGW	2 cut(s)	866, 1830
BsaXI	ACNNNNNCTCC	6 cut(s)	77, 107, 619, 649, 3264, 3294
Bsc4I	CCNNNNNNNGG	9 cut(s)	680, 697, 698, 866, 880, 1837, 2099, 2253, 2474
Bse118I	RCCGGY	3 cut(s)	866, 1830, 1924
Bse21I	CCTNAGG	2 cut(s)	2514, 3253
Bse3DI	GCAATG	2 cut(s)	2328, 2685
Bse8I	GATNNNNATC	1 cut(s)	195
BseBI	CCWGG	6 cut(s)	631, 1036, 1062, 1367, 1647, 2248
BseCI	ATCGAT	2 cut(s)	199, 1374
BseDI	CCNNGG	7 cut(s)	566, 630, 1000, 1034, 1201, 1339, 2169
BseJI	GATNNNNATC	1 cut(s)	195
BseLI	CCNNNNNNNGG	9 cut(s)	680, 697, 698, 866, 880, 1837, 2099, 2253, 2474
BseMI	GCAATG	2 cut(s)	2328, 2685
BseMII	CTCAG	5 cut(s)	804, 894, 2288, 2505, 2961
BseRI	GAGGAG	2 cut(s)	167, 849
BseSI	GKGCMC	2 cut(s)	714, 1222
BseYI	CCCAGC	3 cut(s)	800, 2641, 3341
BsgI	GTGCAG	2 cut(s)	1698, 1829
Bsh1236I	CGCG	1 cut(s)	3353
BshNI	GGYRCC	2 cut(s)	626, 2089
BshTI	ACCGGT	2 cut(s)	866, 1830
BshVI	ATCGAT	2 cut(s)	199, 1374
BsiHKAI	GWGCWC	3 cut(s)	24, 706, 909
BsiHKCI	CYCGRG	3 cut(s)	505, 2931, 3036
BsiSI	CCGG	5 cut(s)	867, 1094, 1831, 1925, 2905
BslFI	GGGAC	5 cut(s)	656, 1211, 1300, 1720, 1886
BslI	CCNNNNNNNGG	9 cut(s)	680, 697, 698, 866, 880, 1837, 2099, 2253, 2474
BsmAI	GTCTC	1 cut(s)	502
BsmFI	GGGAC	5 cut(s)	656, 1211, 1300, 1720, 1886
BsmI	GAATGC	1 cut(s)	3020
BsoBI	CYCGRG	3 cut(s)	505, 2931, 3036
Bsp120I	GGGCCC	1 cut(s)	710
Bsp1286I	GDGCHC	8 cut(s)	24, 637, 706, 714, 909, 1222, 1280, 1625
Bsp1407I	TGTACA	2 cut(s)	1321, 2389
Bsp143I	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
Bsp1720I	GCTNAGC	1 cut(s)	11
Bsp19I	CCATGG	3 cut(s)	1000, 1339, 2169
BspCNI	CTCAG	5 cut(s)	803, 895, 2289, 2506, 2960
BspDI	ATCGAT	2 cut(s)	199, 1374
BspFNI	CGCG	1 cut(s)	3353
BspHI	TCATGA	2 cut(s)	1140, 2974
BspLI	GGNNCC	8 cut(s)	628, 636, 712, 1199, 1277, 2091, 2303, 3033
BspMAI	CTGCAG	3 cut(s)	835, 1227, 2083
BspPI	GGATC	1 cut(s)	273
BspQI	GCTCTTC	1 cut(s)	741
BspT107I	GGYRCC	2 cut(s)	626, 2089
BspTI	CTTAAG	2 cut(s)	3020, 3196
BsrBI	CCGCTC	1 cut(s)	2292
BsrDI	GCAATG	2 cut(s)	2328, 2685
BsrFI	RCCGGY	3 cut(s)	866, 1830, 1924
BsrGI	TGTACA	2 cut(s)	1321, 2389
BssAI	RCCGGY	3 cut(s)	866, 1830, 1924
BssECI	CCNNGG	7 cut(s)	566, 630, 1000, 1034, 1201, 1339, 2169
BssMI	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
BssNAI	GTATAC	1 cut(s)	1500
BssSI	CACGAG	1 cut(s)	1699
BssT1I	CCWWGG	5 cut(s)	566, 1000, 1201, 1339, 2169
Bst1107I	GTATAC	1 cut(s)	1500
Bst2BI	CACGAG	1 cut(s)	1699
Bst2UI	CCWGG	6 cut(s)	631, 1036, 1062, 1367, 1647, 2248
Bst6I	CTCTTC	3 cut(s)	48, 741, 1024
BstAFI	CTTAAG	2 cut(s)	3020, 3196
BstAPI	GCANNNNNTGC	1 cut(s)	1676
BstAUI	TGTACA	2 cut(s)	1321, 2389
BstC8I	GCNNGC	7 cut(s)	233, 432, 1227, 1846, 1926, 2874, 2967
BstDSI	CCRYGG	3 cut(s)	1000, 1339, 2169
BstEII	GGTNACC	1 cut(s)	869
BstENI	CCTNNNNNAGG	1 cut(s)	678
BstFNI	CGCG	1 cut(s)	3353
BstH2I	RGCGCY	1 cut(s)	850
BstHHI	GCGC	2 cut(s)	849, 3353
BstKTI	GATC	8 cut(s)	268, 923, 1374, 1402, 2149, 2953, 3057, 3379
BstMAI	GTCTC	1 cut(s)	502
BstMBI	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
BstNI	CCWGG	6 cut(s)	631, 1036, 1062, 1367, 1647, 2248
BstNSI	RCATGY	3 cut(s)	1212, 3065, 3281
BstPI	GGTNACC	1 cut(s)	869
BstSCI	CCNGG	8 cut(s)	629, 1034, 1060, 1092, 1365, 1645, 2246, 2904
BstSFI	CTRYAG	3 cut(s)	831, 1223, 2079
BstSLI	GKGCMC	2 cut(s)	714, 1222
BstUI	CGCG	1 cut(s)	3353
BstXI	CCANNNNNNTGG	2 cut(s)	611, 2698
BstZ17I	GTATAC	1 cut(s)	1500
Bsu15I	ATCGAT	2 cut(s)	199, 1374
Bsu36I	CCTNAGG	2 cut(s)	2514, 3253
BsuI	GTATCC	1 cut(s)	3216
BsuTUI	ATCGAT	2 cut(s)	199, 1374
BtgI	CCRYGG	3 cut(s)	1000, 1339, 2169
BtgZI	GCGATG	2 cut(s)	1149, 1863
BtsI	GCAGTG	6 cut(s)	828, 1220, 2088, 2370, 3032, 3129
Cac8I	GCNNGC	7 cut(s)	233, 432, 1227, 1846, 1926, 2874, 2967
CaiI	CAGNNNCTG	2 cut(s)	797, 902
CciI	TCATGA	2 cut(s)	1140, 2974
CfoI	GCGC	2 cut(s)	849, 3353
Cfr10I	RCCGGY	3 cut(s)	866, 1830, 1924
Cfr13I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2111, 3050, 3344
ClaI	ATCGAT	2 cut(s)	199, 1374
CspAI	ACCGGT	2 cut(s)	866, 1830
CspCI	CAANNNNNGTGG	2 cut(s)	261, 296
DpnI	GATC	8 cut(s)	267, 922, 1373, 1401, 2148, 2952, 3056, 3378
DpnII	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
DraI	TTTAAA	1 cut(s)	537
DraIII	CACNNNGTG	1 cut(s)	1096
DriI	GACNNNNNGTC	1 cut(s)	1056
EaeI	YGGCCR	3 cut(s)	816, 1236, 2902
Eam1104I	CTCTTC	3 cut(s)	48, 741, 1024
Eam1105I	GACNNNNNGTC	1 cut(s)	1056
EarI	CTCTTC	3 cut(s)	48, 741, 1024
Ecl136II	GAGCTC	1 cut(s)	22
Eco130I	CCWWGG	5 cut(s)	566, 1000, 1201, 1339, 2169
Eco147I	AGGCCT	1 cut(s)	2784
Eco24I	GRGCYC	5 cut(s)	24, 637, 714, 1280, 1625
Eco47I	GGWCC	3 cut(s)	1198, 1770, 3050
Eco53kI	GAGCTC	1 cut(s)	22
Eco57I	CTGAAG	5 cut(s)	23, 518, 2556, 2873, 3357
Eco81I	CCTNAGG	2 cut(s)	2514, 3253
Eco88I	CYCGRG	3 cut(s)	505, 2931, 3036
Eco91I	GGTNACC	1 cut(s)	869
EcoICRI	GAGCTC	1 cut(s)	22
EcoNI	CCTNNNNNAGG	1 cut(s)	678
EcoO109I	RGGNCCY	2 cut(s)	711, 1198
EcoO65I	GGTNACC	1 cut(s)	869
EcoRII	CCWGG	6 cut(s)	629, 1034, 1060, 1365, 1645, 2246
EcoT14I	CCWWGG	5 cut(s)	566, 1000, 1201, 1339, 2169
EcoT38I	GRGCYC	5 cut(s)	24, 637, 714, 1280, 1625
ErhI	CCWWGG	5 cut(s)	566, 1000, 1201, 1339, 2169
FalI	AAGNNNNNCTT	4 cut(s)	203, 235, 2053, 2085
FaqI	GGGAC	5 cut(s)	656, 1211, 1300, 1720, 1886
FauI	CCCGC	2 cut(s)	255, 1439
FblI	GTMKAC	4 cut(s)	600, 1499, 2205, 2449
FriOI	GRGCYC	5 cut(s)	24, 637, 714, 1280, 1625
FspBI	CTAG	6 cut(s)	852, 2217, 2334, 2877, 2898, 3348
GlaI	GCGC	2 cut(s)	848, 3352
GsaI	CCCAGC	3 cut(s)	804, 2645, 3345
GsuI	CTGGAG	2 cut(s)	1388, 3381
HaeII	RGCGCY	1 cut(s)	850
HapII	CCGG	5 cut(s)	867, 1094, 1831, 1925, 2905
HhaI	GCGC	2 cut(s)	849, 3353
Hin6I	GCGC	2 cut(s)	847, 3351
HinP1I	GCGC	2 cut(s)	847, 3351
HincII	GTYRAC	5 cut(s)	357, 1633, 1888, 2206, 2737
HindII	GTYRAC	5 cut(s)	357, 1633, 1888, 2206, 2737
HindIII	AAGCTT	2 cut(s)	249, 3193
HpaI	GTTAAC	1 cut(s)	1888
HpaII	CCGG	5 cut(s)	867, 1094, 1831, 1925, 2905
HphI	GGTGA	4 cut(s)	145, 413, 765, 1355
Hpy99I	CGWCG	1 cut(s)	1579
HpyAV	CCTTC	9 cut(s)	178, 1712, 1748, 1949, 2080, 2124, 2478, 2800, 3296
HspAI	GCGC	2 cut(s)	847, 3351
KpnI	GGTACC	1 cut(s)	630
KroI	GCCGGC	1 cut(s)	1924
KroNI	GCCGGC	1 cut(s)	1926
KspAI	GTTAAC	1 cut(s)	1888
Kzo9I	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
LguI	GCTCTTC	1 cut(s)	741
MaeI	CTAG	6 cut(s)	852, 2217, 2334, 2877, 2898, 3348
MaeIII	GTNAC	5 cut(s)	133, 646, 869, 1353, 1438
MalI	GATC	8 cut(s)	267, 922, 1373, 1401, 2148, 2952, 3056, 3378
MbiI	CCGCTC	1 cut(s)	2292
MboI	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
MboII	GAAGA	9 cut(s)	65, 223, 511, 728, 1011, 2700, 2811, 2879, 3119
MhlI	GDGCHC	8 cut(s)	24, 637, 706, 714, 909, 1222, 1280, 1625
MlsI	TGGCCA	1 cut(s)	1238
MluNI	TGGCCA	1 cut(s)	1238
MlyI	GAGTC	5 cut(s)	101, 1452, 2240, 2546, 3305
MmeI	TCCRAC	4 cut(s)	63, 764, 1099, 2155
Mox20I	TGGCCA	1 cut(s)	1238
MroNI	GCCGGC	1 cut(s)	1924
MroXI	GAANNNNTTC	1 cut(s)	215
MscI	TGGCCA	1 cut(s)	1238
MslI	CAYNNNNRTG	3 cut(s)	609, 1466, 2696
Msp20I	TGGCCA	1 cut(s)	1238
MspA1I	CMGCKG	3 cut(s)	731, 1429, 2645
MspCI	CTTAAG	2 cut(s)	3020, 3196
MspI	CCGG	5 cut(s)	867, 1094, 1831, 1925, 2905
MspR9I	CCNGG	8 cut(s)	631, 1036, 1062, 1094, 1367, 1647, 2248, 2906
MssI	GTTTAAAC	1 cut(s)	537
Mva1269I	GAATGC	1 cut(s)	3020
MvaI	CCWGG	6 cut(s)	631, 1036, 1062, 1367, 1647, 2248
MvnI	CGCG	1 cut(s)	3353
NaeI	GCCGGC	1 cut(s)	1926
NciI	CCSGG	2 cut(s)	1094, 2906
NcoI	CCATGG	3 cut(s)	1000, 1339, 2169
NdeII	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
NgoMIV	GCCGGC	1 cut(s)	1924
NlaIV	GGNNCC	8 cut(s)	628, 636, 712, 1199, 1277, 2091, 2303, 3033
NmuCI	GTSAC	3 cut(s)	133, 646, 1438
NspI	RCATGY	3 cut(s)	1212, 3065, 3281
OliI	CACNNNNGTG	1 cut(s)	609
PaeR7I	CTCGAG	2 cut(s)	505, 3036
PagI	TCATGA	2 cut(s)	1140, 2974
PceI	AGGCCT	1 cut(s)	2784
PciSI	GCTCTTC	1 cut(s)	741
PctI	GAATGC	1 cut(s)	3020
PdiI	GCCGGC	1 cut(s)	1926
PdmI	GAANNNNTTC	1 cut(s)	215
PfeI	GAWTC	5 cut(s)	226, 899, 1724, 2705, 2831
PflMI	CCANNNNNTGG	1 cut(s)	2253
PinAI	ACCGGT	2 cut(s)	866, 1830
PleI	GAGTC	5 cut(s)	100, 1452, 2239, 2546, 3304
PmeI	GTTTAAAC	1 cut(s)	537
PpsI	GAGTC	5 cut(s)	100, 1452, 2239, 2546, 3304
PpuMI	RGGWCCY	1 cut(s)	1198
PshBI	ATTAAT	1 cut(s)	525
Psp124BI	GAGCTC	1 cut(s)	24
Psp5II	RGGWCCY	1 cut(s)	1198
Psp6I	CCWGG	6 cut(s)	629, 1034, 1060, 1365, 1645, 2246
PspEI	GGTNACC	1 cut(s)	869
PspFI	CCCAGC	3 cut(s)	800, 2641, 3341
PspGI	CCWGG	6 cut(s)	629, 1034, 1060, 1365, 1645, 2246
PspN4I	GGNNCC	8 cut(s)	628, 636, 712, 1199, 1277, 2091, 2303, 3033
PspOMI	GGGCCC	1 cut(s)	710
PspPI	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2111, 3050, 3344
PspPPI	RGGWCCY	1 cut(s)	1198
PsrI	GAACNNNNNNTAC	2 cut(s)	847, 879
PstI	CTGCAG	3 cut(s)	835, 1227, 2083
PstNI	CAGNNNCTG	2 cut(s)	797, 902
PvuII	CAGCTG	3 cut(s)	731, 1429, 2645
RseI	CAYNNNNRTG	3 cut(s)	609, 1466, 2696
SacI	GAGCTC	1 cut(s)	24
SalI	GTCGAC	1 cut(s)	2204
SapI	GCTCTTC	1 cut(s)	741
Sau3AI	GATC	8 cut(s)	265, 920, 1371, 1399, 2146, 2950, 3054, 3376
Sau96I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2111, 3050, 3344
ScaI	AGTACT	1 cut(s)	1903
SchI	GAGTC	5 cut(s)	101, 1452, 2240, 2546, 3305
ScrFI	CCNGG	8 cut(s)	631, 1036, 1062, 1094, 1367, 1647, 2248, 2906
SduI	GDGCHC	8 cut(s)	24, 637, 706, 714, 909, 1222, 1280, 1625
SfcI	CTRYAG	3 cut(s)	831, 1223, 2079
Sfr274I	CTCGAG	2 cut(s)	505, 3036
SinI	GGWCC	3 cut(s)	1198, 1770, 3050
SlaI	CTCGAG	2 cut(s)	505, 3036
SmiMI	CAYNNNNRTG	3 cut(s)	609, 1466, 2696
SmlI	CTYRAG	7 cut(s)	505, 1624, 2670, 3020, 3036, 3196, 3284
SmoI	CTYRAG	7 cut(s)	505, 1624, 2670, 3020, 3036, 3196, 3284
SseBI	AGGCCT	1 cut(s)	2784
SspI	AATATT	2 cut(s)	1962, 2481
SspMI	CTAG	6 cut(s)	852, 2217, 2334, 2877, 2898, 3348
SstI	GAGCTC	1 cut(s)	24
StuI	AGGCCT	1 cut(s)	2784
StyD4I	CCNGG	8 cut(s)	629, 1034, 1060, 1092, 1365, 1645, 2246, 2904
StyI	CCWWGG	5 cut(s)	566, 1000, 1201, 1339, 2169
TaqI	TCGA	9 cut(s)	199, 268, 506, 1374, 1574, 2149, 2205, 3037, 3330
TatI	WGTACW	4 cut(s)	1321, 1901, 2389, 3280
TauI	GCSGC	5 cut(s)	821, 2078, 2292, 3224, 3371
TfiI	GAWTC	5 cut(s)	226, 899, 1724, 2705, 2831
TseFI	GTSAC	3 cut(s)	133, 646, 1438
Tsp45I	GTSAC	3 cut(s)	133, 646, 1438
TspGWI	ACGGA	2 cut(s)	956, 2870
Van91I	CCANNNNNTGG	1 cut(s)	2253
Vha464I	CTTAAG	2 cut(s)	3020, 3196
VpaK11BI	GGWCC	3 cut(s)	1198, 1770, 3050
VspI	ATTAAT	1 cut(s)	525
XagI	CCTNNNNNAGG	1 cut(s)	678
XapI	RAATTY	6 cut(s)	59, 1104, 1865, 1995, 2350, 2882
XbaI	TCTAGA	1 cut(s)	2333
XceI	RCATGY	3 cut(s)	1212, 3065, 3281
XhoI	CTCGAG	2 cut(s)	505, 3036
XmiI	GTMKAC	4 cut(s)	600, 1499, 2205, 2449
XmnI	GAANNNNTTC	1 cut(s)	215
XspI	CTAG	6 cut(s)	852, 2217, 2334, 2877, 2898, 3348
ZrmI	AGTACT	1 cut(s)	1903

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.