Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001857

Physical Location & Seq

Genomic Coordinates Forward (+)

3849670 .. 3861067

Gene Structure 11398 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1043800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3390 bp

ATGGCGGCAAGGAGAGAGCTCGTTTTTCTAATTCTTCAGTATTTGGAGGAAGACAAATACATGGAGACTGTTCACAGGTTCGAGAAAGAGTCCGGGTTTTTCTTCAATATGAAGTATCTTGAGGAAATTGTGACAAATGGTGATTGGGAGGAGGTGGAGAACTACTTGTCAGGATTTACTAAGGTTGAGGACAATAGACACTCATCGAAGATTTTCTTTGAGATTCGGAAACAAAGATACCTTGAGGCTCTTGACAGTCATGATAATGATAAAGCTGTTGAGATTCTTCAGAAGGATTTGAAAGCATTCTCGTCTATCAATCCAGAGATTTTCAAGGAAATAACGATGCTTCTAACGTTGGAGAATTTCAGGGAGAATGAGCAACTATCAAGCTATGGTGATGCTAAGATTGGCAGGGCTATAATGCTTGCTGAACTGAAAAAGGTAATTGAGGCAAATCCAATTTTCCATGATAAGCTTGAATTCCCCAACTTTAAGAAGTCAAGGCTGAGGACATTGATCAATCAGAGTTTAAACTGGCAGCACCATCTTTGTAATAATCCAAGGCAAAACCCTGACATAAAAACTCTATTTTGGGACCATAGTTGTGGACCAGGTGCTTGTGCTACTTCCCCTGCAATTAACCCTTTAATGGGATCCCTTCCAAAAGTAGGTGGCTTTCTACCAATAGGTGCTCATGGTCCTGCAGCACCTCCTTCAGCGTTTCTGCCTGGCTGGATGGCTAACCCATACTCGGCTCCACACCAAACAGTTTCTTCTGGACCAATCGGTTTAACAGTTCCCAATAATACATCATCTTTGCTAAAGCGCCCAAGGACTCCTCCTAATAACCTGGCAATGGACTATCAAACTGCAGATTCTGGACATGTGTCCAAGAGATCAAGAGCGTTGGGAACATCAGATGAGGTCAATAATAGGTCTCTTAACGTCTTTCCACACACATATCCGGGCCACATCCATGCCCACACCCCTTACTCTTCTGAGGACTTGCCAAAGACTGTCGTTGTCAATTTGACTCTAGGTTCAGCTGCCAAGAGCTTGGAATTTCATCCCCTGCAACGCGCTCTGCTTCTTGTTGGAACAAATATTGGTGACATAACGATTTGGGAAGTAGGCAGAAGGGAGAGGCTTGTTTCTCGGAATTTCAAAATTTGGGATCTTTCTGTGTGTTCAATGGCTTTGCAGGCGTCTTTGGCAAATGAATACACTGCATCAGTAAATCGTGTGATCTGGAGTCCTGATGGTTCCCACGTTGGTATTGCATATTCGAAGCATGTAGTGCACATATATTCCTATCATGGTGGTGATGATTTACGGAACCACCTAGAGATTGATGCTCATGCTGGTAATGTTAGTGATCTTGCTTTCACTCACCTAAACAAAAAATTATGCATCATAAGTTGCGGAGAGGACAAGACTACTAAGGTCTGGGATGCTGTCACTGGCAATAAACTGTATAACTTTGAAGGTCATGAAGCACCAGTTTATTCTGTGTGTGAACACATAAAGGACAATGTTCAGTTCATCTTCGCAACAGCTATTGATGGGAAAATTAAGGCATGGCTGTATGATAACGTTGGTTCTAGAGTTGATTACCATGCTCCAGGCCATTCTTGCACAAGAATGGTGTATAGTACTGATGGTACAAGGCTCTTCTCGTGTGGGACTAATAAGGAAGGGGAATCATACATTGTGGAATGGAATGAAAACGAGGGGACTGTAAAGCGAACATATAATGGGCTTGGCAAGCAAGCTCATGGTGTTGTGCAGTTTGATACGACGAAGAACCGATTCTTGGCTGCTGGTGATGATTTCCAAATCAAATATTGGGACATGGACAATGTGAACCTTTTGAATGCCACTGATGCGGAGGGTGGATTACTGGCATCTCCTTGTCTACGATTTAACAAGGAAGGAACTATGCTAGCTGTTTCAACAAGTGAGAATGGTGTTAAAGTACTTGCAAATGCCGACGGCATTCGAATTTTACGTGCAATTGAGAATTGTGCAGTTGATGCCTCCCGGATGGCTTCAGGAACTGCTATGGAGGGACCCATAATTGGAACATCTGGTGCTTCCAGTTCTGCTCATGGAACAAACAATGCGGTTGCAGATAGAAGTCTGAACGGAGATGGCCGGAGTTTTGCAAACATCAAAGTAAGGGTTGCTGAGGAGCTAGAGAAACCAAAAACTTGGAAGCTAACTGAAGTTAATGAACCGTCTCTTCTTCGCTTCATATGGCTGCCTGATAGCCTGTTGGTAAATAGAATTGTTCGGTTAGTATATGCAAATTCAGGAGCATCTATATTGGCATTGACGAGTACTGGGGTACATATACTTTGGAAATGGCAGAAGGATGAGAAGAATGTTTTGGGCAAAGCAACTACCACTGTGCCACCACTGTTATGGCAACCTGCCAGTGGTATAGTGATGACCAATCAAGTACCCGAGACAACTCTTGAAGATCCTATCCATTGCCTTGCTATTTCTAAGAATGATTCTTATGCGCTATCCGCATCTGGAGGGAAGGTTTCGCTTTATAATATGATGAACTTTAAGACAATGATGACCTTTATTGCCTCACCACCTGCAGCAACATTTCTTGCCTTCCATCCGCAGGACAACAACATTATTGCAGTGGGCATGGATGATTCTACAATTCAAATCTACAATGTCCGAACTGACGAGGTCCAGAACAAGCTCAAAGGTCATCAAAGGAGAGTTACTGGCCTAGCATTTTCCAATGTTCTAAATGTTCTTGTATCAGCAGGAGCTGATTCTCAGCTGTGTACATGGAGCATTGACGGATGGAGAAAGCAGGCTGCTAAGTTCTTGCAGATTCCAACTGGTCGCGTGCCTACGCCTAATGCTCAGACCCGTGTTCAGTTTCACCAAGATCAGATACATATGTTAGCTGCTCATGCAACTCAAATAGCCATATATGAAGCACCGAAGTTGGAGTGCCTTAAACAGTGGGTGCCACGAGAATCGATTGGTCCTCTCGCCGATGCTACCTATTCATGTGATAGCCAGTCAATTTATGCAAGCTTTGAGGATGGAAGTGTATGCATTCTAACTGCCTCTACACTCCGAATCAGATATCGGATAAGCACTCCTGCATATGTGAAGTCTAGTCCCAGCACGAAGGTCTACCCAGCTGTGGTTGCTGCTCATCCATCCGACCCTAACCAATTCGCCCTTGGACTCACCAATGGGGGAGTTGTGGTGATGGAGCCACCGGAATCAGAGGGGACGTGGGGTACTATGCCTCCAGTTGAGAATGCAGGCCCTAATGCCAGCTCCGTGGTGGTTCGGATCAACAGCCAAGGTGACAGTGACTCTTTGGTTTTCGCAGCGGGTAGGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1130

Amino Acids

124.5

Weight (kDa)

6.49

Isoelectric Point (pI)

37.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 33	2.1e-06	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	36 - 160	1.3e-55	TPR1-like, CTLH-containing domain
zf_topless	PF21359	163 - 203	1.4e-22	TOPLESS, zinc finger domain
Beta-prop_WDR3_1st	PF25173	388 - 567	7.6e-18	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	389 - 529	1.5e-12	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	390 - 564	4.8e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	410 - 668	9.8e-21	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	413 - 625	1.2e-06	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	424 - 531	7.2e-08	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	431 - 528	1e-06	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	449 - 657	3.1e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	470 - 660	8.5e-08	Prp19 WD40 domain
EIF3I	PF24805	472 - 665	8.2e-07	EIF3I
Beta-prop_EML_2	PF23414	821 - 946	1.6e-12	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	824 - 943	1.5e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	824 - 942	1.1e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	826 - 1050	5.2e-13	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	826 - 1038	1.1e-15	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	827 - 942	5.7e-16	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	827 - 942	6.5e-12	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	827 - 1023	9.8e-16	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	829 - 1033	4.5e-17	WDR5 beta-propeller domain
WDR55	PF24796	831 - 942	3.2e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	831 - 948	1.4e-11	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	845 - 1032	2.9e-11	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	849 - 946	9.4e-09	WDR90/POC16, second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15880 AT3G15880 AT3G15880 AT3G15880
fragaria_vesca	FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730
malus_domestica	MD02G1027700.v1.1 MD11G1043800.v1.1 MD15G1169300.v1.1
prunus_persica	Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1
pyrus_communis	pycom12419g00020
rosa_chinensis	RchiOBHm_Chr2g0087841
rosa_laevigata	RLG00000015906 RLG00000019456
rosa_multiflora	Rmu_sc0008385.1_g000016
rosa_roxburghii	Rroxscaffold_2G00153180
rosa_rugosa	Rorug01G0479000 Rorug01G0479000 Rorug02G0324100 Rorug02G0324100
rosa_samantha	Rh2BG032700
rosa_wichuraiana	Rw2G002610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2562
AarI	CACCTGC	1 cut(s)	2617
Acc36I	ACCTGC	2 cut(s)	2443, 2617
AccB1I	GGYRCC	1 cut(s)	2998
AccI	GTMKAC	2 cut(s)	1918, 3171
AccII	CGCG	2 cut(s)	1083, 2874
AciI	CCGC	7 cut(s)	5, 1425, 1889, 2126, 2535, 2636, 3377
AclI	AACGTT	2 cut(s)	356, 1596
AclWI	GGATC	5 cut(s)	651, 664, 1185, 2480, 3344
AcoI	YGGCCR	1 cut(s)	2155
AcsI	RAATTY	7 cut(s)	364, 482, 1064, 1162, 1170, 2004, 2311
AcuI	CTGAAG	5 cut(s)	20, 272, 702, 2037, 2247
AcyI	GRCGYC	1 cut(s)	1208
AfaI	GTAC	8 cut(s)	1657, 1666, 1980, 2344, 2352, 2466, 2812, 3283
AflIII	ACRYGT	1 cut(s)	886
AjiI	CACGTC	1 cut(s)	3276
AjnI	CCWGG	4 cut(s)	613, 730, 852, 1624
AjuI	GAANNNNNNNTTGG	4 cut(s)	1799, 1831, 2375, 2407
Alw21I	GWGCWC	3 cut(s)	21, 697, 1305
Alw26I	GTCTC	4 cut(s)	59, 945, 2247, 2465
Alw44I	GTGCAC	1 cut(s)	1301
AlwI	GGATC	5 cut(s)	651, 664, 1185, 2480, 3344
AlwNI	CAGNNNCTG	3 cut(s)	881, 2060, 2795
Ama87I	CYCGRG	1 cut(s)	2468
AoxI	GGCC	5 cut(s)	970, 1627, 2155, 2749, 3307
ApaLI	GTGCAC	1 cut(s)	1301
ApoI	RAATTY	7 cut(s)	364, 482, 1064, 1162, 1170, 2004, 2311
Asp700I	GAANNNNTTC	3 cut(s)	212, 305, 1811
AspLEI	GCGC	3 cut(s)	831, 1085, 2530
AspS9I	GGNCC	9 cut(s)	598, 611, 701, 782, 970, 2072, 2710, 3017, 3308
AsuC2I	CCSGG	3 cut(s)	94, 969, 2044
AsuII	TTCGAA	2 cut(s)	1289, 2002
AsuNHI	GCTAGC	1 cut(s)	1945
AvaI	CYCGRG	1 cut(s)	2468
AvaII	GGWCC	7 cut(s)	598, 611, 701, 782, 2072, 2710, 3017
BaeGI	GKGCMC	1 cut(s)	1305
BaeI	ACNNNNGTAYC	2 cut(s)	2915, 2948
BamHI	GGATCC	1 cut(s)	656
BanI	GGYRCC	1 cut(s)	2998
BanII	GRGCYC	1 cut(s)	21
BauI	CACGAG	2 cut(s)	1678, 3003
BbsI	GAAGAC	1 cut(s)	57
Bbv12I	GWGCWC	3 cut(s)	21, 697, 1305
BbvCI	CCTCAGC	2 cut(s)	509, 2190
BceAI	ACGGC	1 cut(s)	2011
BciT130I	CCWGG	4 cut(s)	615, 732, 854, 1626
BclI	TGATCA	1 cut(s)	519
BcnI	CCSGG	3 cut(s)	94, 969, 2044
BcoDI	GTCTC	4 cut(s)	59, 945, 2247, 2465
BfaI	CTAG	7 cut(s)	1040, 1346, 1605, 1946, 2198, 2753, 3153
BfmI	CTRYAG	3 cut(s)	705, 873, 2610
BfoI	RGCGCY	1 cut(s)	832
BfuAI	ACCTGC	2 cut(s)	2443, 2617
BmcAI	AGTACT	3 cut(s)	1657, 1980, 2344
Bme1390I	CCNGG	7 cut(s)	94, 615, 732, 854, 969, 1626, 2044
Bme18I	GGWCC	7 cut(s)	598, 611, 701, 782, 2072, 2710, 3017
BmeT110I	CYCGRG	1 cut(s)	2468
BmgBI	CACGTC	1 cut(s)	3276
BmgT120I	GGNCC	9 cut(s)	598, 611, 701, 782, 970, 2072, 2710, 3017, 3308
BmiI	GGNNCC	9 cut(s)	599, 658, 759, 1267, 1340, 2073, 2074, 3000, 3255
BmrFI	CCNGG	7 cut(s)	94, 615, 732, 854, 969, 1626, 2044
BmrI	ACTGGG	1 cut(s)	2355
BmtI	GCTAGC	1 cut(s)	1949
BmuI	ACTGGG	1 cut(s)	2355
BoxI	GACNNNNGTC	1 cut(s)	889
BpiI	GAAGAC	1 cut(s)	57
BpmI	CTGGAG	4 cut(s)	1273, 1608, 2562, 3276
Bpu10I	CCTNAGC	2 cut(s)	509, 2190
Bpu14I	TTCGAA	2 cut(s)	1289, 2002
BpuEI	CTTGAG	2 cut(s)	140, 263
BpuMI	CCSGG	3 cut(s)	94, 969, 2044
Bsa29I	ATCGAT	1 cut(s)	3011
BsaAI	YACGTR	1 cut(s)	2012
BsaHI	GRCGYC	1 cut(s)	1208
BsaI	GGTCTC	1 cut(s)	945
BsaJI	CCNNGG	5 cut(s)	563, 833, 3220, 3324, 3346
BsaWI	WCCGGW	1 cut(s)	3259
BsaXI	ACNNNNNCTCC	6 cut(s)	2535, 2565, 3231, 3261, 3274, 3304
Bse3DI	GCAATG	2 cut(s)	864, 2494
BseBI	CCWGG	4 cut(s)	615, 732, 854, 1626
BseCI	ATCGAT	1 cut(s)	3011
BseDI	CCNNGG	5 cut(s)	563, 833, 3220, 3324, 3346
BseMI	GCAATG	2 cut(s)	864, 2494
BseMII	CTCAG	5 cut(s)	500, 993, 2181, 2816, 2906
BseRI	GAGGAG	3 cut(s)	164, 831, 2207
BseSI	GKGCMC	1 cut(s)	1305
BseYI	CCCAGC	2 cut(s)	3158, 3175
BsgI	GTGCAG	2 cut(s)	1808, 2049
Bsh1236I	CGCG	2 cut(s)	1083, 2874
BshFI	GGCC	5 cut(s)	972, 1629, 2157, 2751, 3309
BshNI	GGYRCC	1 cut(s)	2998
BshVI	ATCGAT	1 cut(s)	3011
BsiHKAI	GWGCWC	3 cut(s)	21, 697, 1305
BsiHKCI	CYCGRG	1 cut(s)	2468
BsiSI	CCGG	5 cut(s)	93, 968, 2044, 2158, 3260
BslFI	GGGAC	7 cut(s)	611, 1699, 1750, 1865, 2085, 3141, 3286
BsmAI	GTCTC	4 cut(s)	59, 945, 2247, 2465
BsmBI	CGTCTC	1 cut(s)	2247
BsmFI	GGGAC	7 cut(s)	611, 1699, 1750, 1865, 2085, 3141, 3286
BsmI	GAATGC	5 cut(s)	305, 1882, 1998, 3090, 3307
BsnI	GGCC	5 cut(s)	972, 1629, 2157, 2751, 3309
Bso31I	GGTCTC	1 cut(s)	945
BsoBI	CYCGRG	1 cut(s)	2468
Bsp119I	TTCGAA	2 cut(s)	1289, 2002
Bsp1286I	GDGCHC	3 cut(s)	21, 697, 1305
Bsp1407I	TGTACA	1 cut(s)	2810
Bsp143I	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
BspACI	CCGC	7 cut(s)	5, 1425, 1889, 2126, 2535, 2636, 3377
BspANI	GGCC	5 cut(s)	972, 1629, 2157, 2751, 3309
BspCNI	CTCAG	5 cut(s)	501, 994, 2182, 2815, 2905
BspDI	ATCGAT	1 cut(s)	3011
BspFNI	CGCG	2 cut(s)	1083, 2874
BspHI	TCATGA	2 cut(s)	259, 1492
BspLI	GGNNCC	9 cut(s)	599, 658, 759, 1267, 1340, 2073, 2074, 3000, 3255
BspMAI	CTGCAG	3 cut(s)	709, 877, 2614
BspMI	ACCTGC	2 cut(s)	2443, 2617
BspOI	GCTAGC	1 cut(s)	1949
BspPI	GGATC	5 cut(s)	651, 664, 1185, 2480, 3344
BspQI	GCTCTTC	1 cut(s)	1679
BspT104I	TTCGAA	2 cut(s)	1289, 2002
BspT107I	GGYRCC	1 cut(s)	2998
BspTNI	GGTCTC	1 cut(s)	945
BsrDI	GCAATG	2 cut(s)	864, 2494
BsrGI	TGTACA	1 cut(s)	2810
BssECI	CCNNGG	5 cut(s)	563, 833, 3220, 3324, 3346
BssMI	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
BssNI	GRCGYC	1 cut(s)	1208
BssSI	CACGAG	2 cut(s)	1678, 3003
BssT1I	CCWWGG	4 cut(s)	563, 833, 3220, 3346
Bst2BI	CACGAG	2 cut(s)	1678, 3003
Bst2UI	CCWGG	4 cut(s)	615, 732, 854, 1626
Bst6I	CTCTTC	3 cut(s)	1003, 1679, 2250
BstACI	GRCGYC	1 cut(s)	1208
BstAPI	GCANNNNNTGC	2 cut(s)	1300, 2036
BstAUI	TGTACA	1 cut(s)	2810
BstBAI	YACGTR	1 cut(s)	2012
BstBI	TTCGAA	2 cut(s)	1289, 2002
BstDSI	CCRYGG	1 cut(s)	3324
BstFNI	CGCG	2 cut(s)	1083, 2874
BstH2I	RGCGCY	1 cut(s)	832
BstHHI	GCGC	3 cut(s)	831, 1085, 2530
BstKTI	GATC	9 cut(s)	522, 659, 902, 1180, 1251, 1381, 2488, 2920, 3339
BstMAI	GTCTC	4 cut(s)	59, 945, 2247, 2465
BstMBI	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
BstNI	CCWGG	4 cut(s)	615, 732, 854, 1626
BstNSI	RCATGY	2 cut(s)	890, 1298
BstPAI	GACNNNNGTC	1 cut(s)	889
BstSCI	CCNGG	7 cut(s)	92, 613, 730, 852, 967, 1624, 2042
BstSFI	CTRYAG	3 cut(s)	705, 873, 2610
BstSLI	GKGCMC	1 cut(s)	1305
BstUI	CGCG	2 cut(s)	1083, 2874
BstV2I	GAAGAC	1 cut(s)	57
BstX2I	RGATCY	3 cut(s)	656, 1177, 2485
BstXI	CCANNNNNNTGG	5 cut(s)	608, 1060, 2214, 2427, 3325
BstYI	RGATCY	3 cut(s)	656, 1177, 2485
Bsu15I	ATCGAT	1 cut(s)	3011
BsuRI	GGCC	5 cut(s)	972, 1629, 2157, 2751, 3309
BsuTUI	ATCGAT	1 cut(s)	3011
BtgI	CCRYGG	1 cut(s)	3324
BtrI	CACGTC	1 cut(s)	3276
BtsI	GCAGTG	2 cut(s)	1227, 2664
BtsIMutI	CAGTG	9 cut(s)	1227, 1461, 1881, 2409, 2420, 2446, 2664, 2999, 3361
BveI	ACCTGC	2 cut(s)	2443, 2617
CaiI	CAGNNNCTG	3 cut(s)	881, 2060, 2795
CciI	TCATGA	2 cut(s)	259, 1492
CfoI	GCGC	3 cut(s)	831, 1085, 2530
Cfr13I	GGNCC	9 cut(s)	598, 611, 701, 782, 970, 2072, 2710, 3017, 3308
ClaI	ATCGAT	1 cut(s)	3011
CseI	GACGC	1 cut(s)	1197
CsiI	ACCWGGT	1 cut(s)	613
Csp6I	GTAC	8 cut(s)	1656, 1665, 1979, 2343, 2351, 2465, 2811, 3282
CviQI	GTAC	8 cut(s)	1656, 1665, 1979, 2343, 2351, 2465, 2811, 3282
DpnI	GATC	9 cut(s)	521, 658, 901, 1179, 1250, 1380, 2487, 2919, 3338
DpnII	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
DraI	TTTAAA	1 cut(s)	534
EaeI	YGGCCR	1 cut(s)	2155
Eam1104I	CTCTTC	3 cut(s)	1003, 1679, 2250
EarI	CTCTTC	3 cut(s)	1003, 1679, 2250
Ecl136II	GAGCTC	1 cut(s)	19
Eco130I	CCWWGG	4 cut(s)	563, 833, 3220, 3346
Eco24I	GRGCYC	1 cut(s)	21
Eco31I	GGTCTC	1 cut(s)	945
Eco32I	GATATC	1 cut(s)	3122
Eco47I	GGWCC	7 cut(s)	598, 611, 701, 782, 2072, 2710, 3017
Eco53kI	GAGCTC	1 cut(s)	19
Eco57I	CTGAAG	5 cut(s)	20, 272, 702, 2037, 2247
Eco88I	CYCGRG	1 cut(s)	2468
EcoICRI	GAGCTC	1 cut(s)	19
EcoO109I	RGGNCCY	2 cut(s)	2072, 3308
EcoRI	GAATTC	1 cut(s)	482
EcoRII	CCWGG	4 cut(s)	613, 730, 852, 1624
EcoRV	GATATC	1 cut(s)	3122
EcoT14I	CCWWGG	4 cut(s)	563, 833, 3220, 3346
EcoT22I	ATGCAT	2 cut(s)	1415, 3092
EcoT38I	GRGCYC	1 cut(s)	21
ErhI	CCWWGG	4 cut(s)	563, 833, 3220, 3346
Esp3I	CGTCTC	1 cut(s)	2247
FalI	AAGNNNNNCTT	2 cut(s)	200, 232
FaqI	GGGAC	7 cut(s)	611, 1699, 1750, 1865, 2085, 3141, 3286
FauI	CCCGC	1 cut(s)	3370
FauNDI	CATATG	3 cut(s)	2258, 2928, 3142
FbaI	TGATCA	1 cut(s)	519
FblI	GTMKAC	2 cut(s)	1918, 3171
FriOI	GRGCYC	1 cut(s)	21
FspBI	CTAG	7 cut(s)	1040, 1346, 1605, 1946, 2198, 2753, 3153
GlaI	GCGC	3 cut(s)	830, 1084, 2529
GsaI	CCCAGC	2 cut(s)	3162, 3179
GsuI	CTGGAG	4 cut(s)	1273, 1608, 2562, 3276
HaeII	RGCGCY	1 cut(s)	832
HaeIII	GGCC	5 cut(s)	972, 1629, 2157, 2751, 3309
HapII	CCGG	5 cut(s)	93, 968, 2044, 2158, 3260
HgaI	GACGC	1 cut(s)	1197
HhaI	GCGC	3 cut(s)	831, 1085, 2530
Hin1I	GRCGYC	1 cut(s)	1208
Hin6I	GCGC	3 cut(s)	829, 1083, 2528
HinP1I	GCGC	3 cut(s)	829, 1083, 2528
HindIII	AAGCTT	2 cut(s)	476, 3067
HpaII	CCGG	5 cut(s)	93, 968, 2044, 2158, 3260
Hpy166II	GTNNAC	8 cut(s)	73, 611, 1303, 1520, 1867, 1919, 2811, 3172
Hpy8I	GTNNAC	8 cut(s)	73, 611, 1303, 1520, 1867, 1919, 2811, 3172
Hpy99I	CGWCG	2 cut(s)	1804, 1997
HpyCH4IV	ACGT	6 cut(s)	356, 948, 1272, 1596, 2011, 3275
HpySE526I	ACGT	6 cut(s)	356, 948, 1272, 1596, 2011, 3275
Hsp92I	GRCGYC	1 cut(s)	1208
HspAI	GCGC	3 cut(s)	829, 1083, 2528
KflI	GGGWCCC	1 cut(s)	2072
Ksp22I	TGATCA	1 cut(s)	519
Kzo9I	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
LguI	GCTCTTC	1 cut(s)	1679
LmnI	GCTCC	8 cut(s)	763, 1627, 2194, 2318, 2792, 2817, 3253, 3326
MabI	ACCWGGT	1 cut(s)	613
MaeI	CTAG	7 cut(s)	1040, 1346, 1605, 1946, 2198, 2753, 3153
MaeII	ACGT	6 cut(s)	356, 948, 1272, 1596, 2011, 3275
MaeIII	GTNAC	6 cut(s)	130, 1112, 1459, 2743, 3350, 3356
MalI	GATC	9 cut(s)	521, 658, 901, 1179, 1250, 1380, 2487, 2919, 3338
MboI	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
MfeI	CAATTG	1 cut(s)	2016
MflI	RGATCY	3 cut(s)	656, 1177, 2485
MhlI	GDGCHC	3 cut(s)	21, 697, 1305
MlyI	GAGTC	6 cut(s)	98, 832, 1030, 1264, 3219, 3353
MmeI	TCCRAC	5 cut(s)	339, 1078, 2888, 2958, 3225
Mph1103I	ATGCAT	2 cut(s)	1415, 3092
MroXI	GAANNNNTTC	3 cut(s)	212, 305, 1811
MslI	CAYNNNNRTG	6 cut(s)	264, 606, 978, 1323, 1643, 2425
MspA1I	CMGCKG	4 cut(s)	1049, 2806, 3179, 3377
MspI	CCGG	5 cut(s)	93, 968, 2044, 2158, 3260
MspR9I	CCNGG	7 cut(s)	94, 615, 732, 854, 969, 1626, 2044
MssI	GTTTAAAC	1 cut(s)	534
MunI	CAATTG	1 cut(s)	2016
Mva1269I	GAATGC	5 cut(s)	305, 1882, 1998, 3090, 3307
MvaI	CCWGG	4 cut(s)	615, 732, 854, 1626
MvnI	CGCG	2 cut(s)	1083, 2874
NciI	CCSGG	3 cut(s)	94, 969, 2044
NdeI	CATATG	3 cut(s)	2258, 2928, 3142
NdeII	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
NheI	GCTAGC	1 cut(s)	1945
NlaIV	GGNNCC	9 cut(s)	599, 658, 759, 1267, 1340, 2073, 2074, 3000, 3255
NmeAIII	GCCGAG	1 cut(s)	734
NmuCI	GTSAC	5 cut(s)	130, 1112, 1459, 3350, 3356
NsiI	ATGCAT	2 cut(s)	1415, 3092
NspI	RCATGY	2 cut(s)	890, 1298
NspV	TTCGAA	2 cut(s)	1289, 2002
PagI	TCATGA	2 cut(s)	259, 1492
PaqCI	CACCTGC	1 cut(s)	2617
PciI	ACATGT	1 cut(s)	886
PciSI	GCTCTTC	1 cut(s)	1679
PcsI	WCGNNNNNNNCGW	1 cut(s)	2008
PctI	GAATGC	5 cut(s)	305, 1882, 1998, 3090, 3307
PdmI	GAANNNNTTC	3 cut(s)	212, 305, 1811
PflFI	GACNNNGTC	1 cut(s)	2708
PfoI	TCCNGGA	1 cut(s)	2042
PleI	GAGTC	6 cut(s)	97, 832, 1030, 1263, 3219, 3353
PmeI	GTTTAAAC	1 cut(s)	534
PpsI	GAGTC	6 cut(s)	97, 832, 1030, 1263, 3219, 3353
Ppu21I	YACGTR	1 cut(s)	2012
PpuMI	RGGWCCY	1 cut(s)	2072
PscI	ACATGT	1 cut(s)	886
PshAI	GACNNNNGTC	1 cut(s)	889
PsiI	TTATAA	1 cut(s)	2562
Psp124BI	GAGCTC	1 cut(s)	21
Psp1406I	AACGTT	2 cut(s)	356, 1596
Psp5II	RGGWCCY	1 cut(s)	2072
Psp6I	CCWGG	4 cut(s)	613, 730, 852, 1624
PspFI	CCCAGC	2 cut(s)	3158, 3175
PspGI	CCWGG	4 cut(s)	613, 730, 852, 1624
PspN4I	GGNNCC	9 cut(s)	599, 658, 759, 1267, 1340, 2073, 2074, 3000, 3255
PspPI	GGNCC	9 cut(s)	598, 611, 701, 782, 970, 2072, 2710, 3017, 3308
PspPPI	RGGWCCY	1 cut(s)	2072
PstI	CTGCAG	3 cut(s)	709, 877, 2614
PstNI	CAGNNNCTG	3 cut(s)	881, 2060, 2795
PsuI	RGATCY	3 cut(s)	656, 1177, 2485
PsyI	GACNNNGTC	1 cut(s)	2708
PvuII	CAGCTG	3 cut(s)	1049, 2806, 3179
RsaI	GTAC	8 cut(s)	1657, 1666, 1980, 2344, 2352, 2466, 2812, 3283
RsaNI	GTAC	8 cut(s)	1656, 1665, 1979, 2343, 2351, 2465, 2811, 3282
RseI	CAYNNNNRTG	6 cut(s)	264, 606, 978, 1323, 1643, 2425
SacI	GAGCTC	1 cut(s)	21
SapI	GCTCTTC	1 cut(s)	1679
Sau3AI	GATC	9 cut(s)	519, 656, 899, 1177, 1248, 1378, 2485, 2917, 3336
Sau96I	GGNCC	9 cut(s)	598, 611, 701, 782, 970, 2072, 2710, 3017, 3308
ScaI	AGTACT	3 cut(s)	1657, 1980, 2344
SchI	GAGTC	6 cut(s)	98, 832, 1030, 1264, 3219, 3353
ScrFI	CCNGG	7 cut(s)	94, 615, 732, 854, 969, 1626, 2044
SduI	GDGCHC	3 cut(s)	21, 697, 1305
SexAI	ACCWGGT	1 cut(s)	613
SfcI	CTRYAG	3 cut(s)	705, 873, 2610
SfuI	TTCGAA	2 cut(s)	1289, 2002
SinI	GGWCC	7 cut(s)	598, 611, 701, 782, 2072, 2710, 3017
SmiMI	CAYNNNNRTG	6 cut(s)	264, 606, 978, 1323, 1643, 2425
SmlI	CTYRAG	2 cut(s)	119, 242
SmoI	CTYRAG	2 cut(s)	119, 242
SsiI	CCGC	7 cut(s)	5, 1425, 1889, 2126, 2535, 2636, 3377
SspI	AATATT	2 cut(s)	1108, 1847
SspMI	CTAG	7 cut(s)	1040, 1346, 1605, 1946, 2198, 2753, 3153
SstI	GAGCTC	1 cut(s)	21
StyD4I	CCNGG	7 cut(s)	92, 613, 730, 852, 967, 1624, 2042
StyI	CCWWGG	4 cut(s)	563, 833, 3220, 3346
TaiI	ACGT	6 cut(s)	359, 951, 1275, 1599, 2014, 3278
TaqI	TCGA	5 cut(s)	81, 206, 1289, 2002, 3011
TatI	WGTACW	4 cut(s)	1655, 1978, 2342, 2810
TauI	GCSGC	1 cut(s)	8
TscAI	CASTG	9 cut(s)	1234, 1468, 1888, 2416, 2427, 2446, 2664, 2999, 3361
TseFI	GTSAC	5 cut(s)	130, 1112, 1459, 3350, 3356
Tsp45I	GTSAC	5 cut(s)	130, 1112, 1459, 3350, 3356
TspGWI	ACGGA	4 cut(s)	1351, 2163, 2841, 3313
TspRI	CASTG	9 cut(s)	1234, 1468, 1888, 2416, 2427, 2446, 2664, 2999, 3361
Tth111I	GACNNNGTC	1 cut(s)	2708
VneI	GTGCAC	1 cut(s)	1301
VpaK11BI	GGWCC	7 cut(s)	598, 611, 701, 782, 2072, 2710, 3017
XapI	RAATTY	7 cut(s)	364, 482, 1064, 1162, 1170, 2004, 2311
XbaI	TCTAGA	1 cut(s)	1604
XceI	RCATGY	2 cut(s)	890, 1298
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2424, 3218, 3325
XmiI	GTMKAC	2 cut(s)	1918, 3171
XmnI	GAANNNNTTC	3 cut(s)	212, 305, 1811
XspI	CTAG	7 cut(s)	1040, 1346, 1605, 1946, 2198, 2753, 3153
ZrmI	AGTACT	3 cut(s)	1657, 1980, 2344
Zsp2I	ATGCAT	2 cut(s)	1415, 3092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.