Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001857

Physical Location & Seq

Genomic Coordinates Forward (+)

2266611 .. 2274899

Gene Structure 8289 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG032700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3405 bp

ATGTCCTCGCTGAGCAGAGAGCTTGTGTTTCTCATACTTCAGTTTCTGGATGAAGAGAAATTCAAAGAGAGTGTTCACAAGTTGGAGCAAGAGTCAGGGTTTTTCTTTAACATGAGGTACTTTGAAGATATGGTGACAAATGGGGAGTGGGAAGAGGTGGAGAAGTATTTGTCTGGTTTCACCAAGGTTGATGATAATAGATACTCTATGAAGATCTTTTTCGAGATACGCAAGCAGAAGTACCTTGAAGCTTTAGACAAGAGGGATCGAGCAAAAGCTGTGGACATTTTAGTCAAGGACTTGAAAGTGTTTGCAGCATTCAATGAAGAACTTTTTAAGGAAATTACACAGCTGTTGACTCTGGAGAACTTTAGGGATAATGAACAGTTATCTAAGTATGGTGATACAAAGTCTGCAAGGGGTATAATGCTTGCAGAACTGAAAAAGTTAATAGAGGCTAACCCATTGTTTCGTGATAAACTTCAATTTCCTACCCTGAAAAACTCAAGATTGCGGACTCTTATCAATCAGAGCTTAAACTGGCAGCACCAGCTTTGTAAGAATCCAAGGCCTAACCCTGACATAAAGACCCTATTTGTAGACCATAGCTGTGGACAACCGAATGGTGCCAGGGCTCCATCCCCTGTTACTAATCATTTAATGGGGGCAGTCCCAAAGACGGGGGGTTTCCCACCACTTGGTGCTCATGGACCCTTTCAGCCCACACCTGCTGCGCTTCCAACATCTCTTGCTGGATGGATGGCCAACTCATCACCTGTGCCTCATCCCTCTGCTTCCGCCGGGCCGATGGGTTTGGCTGCAGCTAACAATGCAGGAGCCCTCCTAAAGCGCCCTAGAACTCCTCCTACCAATAACCCAGCTATGGACTATCAAACAGCAGAGTCTGAACATGTGATGAAGAGATCAAGACCTTTTGGAGTAACAGATGAGGCAAATAATCTGCCAGTAAATATTCTGCCAGTTGCTTATCCTAACCAGAGCCATGGTCAGAGTTCCTACTCTACTGATGACTTGCCCAGGAATGTAGTCATGACAATAAATCCAGGTTCAGCTGTCAAGAGTATGGATTTTCACCCAGTGCAACAGATTCTACTACTTGTTGGAACAAATATGGGTGATGTCATGATATATGAGTTACCAAGCCATGAAAGAATTGCTATTAGGAACTTCAAAGCCTGGGACCTTGCACAATGTTCAGTGGCTCTGCAGGCAACTTTGGCCAATGATTATACAGCATCGATCAACCGTGTAGTGTGGAGTCCTGATGGAACTCTTTTTGGTGTTGCATACTCTAAGCATCTAGTTCACATTTACTCCTATCATGGTGGTGATGATTTAAGAAATCACCTAGAGATTGAGGCACATGCTGGCAGTGTTAATGATCTTGCTTTCTCATATCCAAACAAACAGCTCTGTGTTGTGACTTGTGGCGAGGATAGGGTTATCAAGGTGTGGGATGCTGTCACTGGGGCCAAGCAGCATACATTTGAGGGCCACGAAGCACCTGTATACTCGGTGTGCCCGCATCACAAGGAGAGTATTCAGTTTATTTTCTCGACAGCAACTGATGGAAAAATAAAGGCATGGCTGTATGATAATGTGGGCTCAAGAGTTGACTATGATGCGCCAGGACATTCGTCTACCACTATGGCATACAGTGCTGATGGATCAAGGCTATTCTCGTGTGGGACAAACAAAGAAGGGGATTCATACTTGGTGGAATGGAATGAAAGTGAAGGAGCTGTAAAGCGTACCTATCATGGTCTTGGGAAGCGGACTGTTGGGGTTGTGCAGTTTGATACCACCAAGAATCGATTCTTGGCTACTGGTGATGAGTTCATGGTTAAATTCTGGGACATGGACAATGTTAACCTTTTGACAAGTACAGATGCAGAAGGTGGACTGCCGGCTTCTCCTTCTATTAGATTTAACAAAGAAGGAATACTGTTAGCTGTCTCAACAAATGACAATGGTATTAAAATTCTAGCAAATTCAGATGGAATTAGGTTGCTGAGAACAGTGGAGAGTCGTACATTTGATGCTTCCAGAGCTGCTTCTGTGGCTGTTGTGAAGGCCCCAGCAATAGGAGCATTTGGATCGTCTAACATCACTGTTGGAACAGGCATTGGAGATCGAGCAGCTCCGGTGCCAGCCATGGTTGGACTGAACAATGACAGTCGAAGTTTGGCTGACGTGAAACCTAGAATTGTTGATGAGTCGATAGAGAAATCCAGGATATGGAAACTGACAGAAATCAATGAATCATCACAATGCCGGTCTCTGAGGCTTCCTGACAATTTAACGGCAATGAGGGTGTCTAGGTTAATTTACACAAATTCAGGACTTGCCATATTGGCATTATCATCTAATGCTGTGCACAAGCTCTGGAAATGGCAACGAAATGACAGAACCACGATAGGGAAGGCGACAGCTAGTATTGTACCACAACTGTGGCAACCTGCAAGTGGCATACTGATGACTAATGATATAAGTGATACAAACCCTGAAGATGCTGTTCCATGCTTTGCACTTTCAAAGAATGACTCTTACGTTATGTCAGCTTCAGGGGGGAAAATATCCCTGTTCAATATGATGACATTTAAGACGATGACAACATTCATGCCTCCACCCCCAGCTGCCACATATCTGGCATTTCATCCCCAAGACAATAATATCATTGCCATAGGCATGGAAGATTCTTCCATCCAGATTTACAATGTTCGGGTTGATGAGGTTAAAACTAAGCTAAAAGGTCATCAGAAAAGAATAACGGGCCTTGCCTTCTCACATGTTCTTAATGTGCTTGTATCCTCGGGAGCCGATTCTCAGTTGTGTGTTTGGAATACAGATGGATGGGAGAGGCAAGCTAGTAAATTCCTTCAGATTCCAAGTGGGCGAGCTGCTGCCCCTCTTGCAGATACTCGGGTTCAGTTCCACTTGGATCAGATACACTTGCTGGCAGTTCATGAAACACAGATAGCCATTTACGAAGCACCAAAATTAGAATGCCTCAAGCAGTGGGTCCCACGAGAGGCTAGTGGTTCAATCACACATGCTGCTTATTCCTGTGATAGCCAGTCAATTTATGTTAGCTTTGAAGATGGAAGTGTGGGGGTTCTTACTGCTACAACCCTCAGGTTGAGATGCCGAATATTACCAGCTGCTTATTTACCTCCAAATCCAAGCTTAAGAGTGTATCCTCTTGTTGTCGCGGCACATCCATCGGATCCCAATCAGTTTGCACTAGGTCTTACAGATGGTGGAGTGCATGTACTTGAGCCATTAGAATCAGAAGGAAAATGGGGCACCTCCCCTCCAACTGAAAATGGTGCTGGGCCTAGCACCGCTTCTGGAGCAGCAGGTTCGGATCAACCTCAAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1134

Amino Acids

124.61

Weight (kDa)

6.54

Isoelectric Point (pI)

39.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	1.6e-12	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.2e-63	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1e-24	TOPLESS, zinc finger domain
Beta-prop_EML_2	PF23414	347 - 474	5.8e-06	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	358 - 573	9.2e-11	WDR3 second beta-propeller domain
Beta-prop_RIG_2nd	PF23775	362 - 512	1.4e-07	RIG second beta-propeller
Beta-prop_WDR3_1st	PF25173	395 - 573	1.8e-16	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	414 - 572	2.3e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	417 - 630	1.9e-19	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	419 - 677	4e-24	THOC3 beta-propeller domain
WDR55	PF24796	419 - 536	6.5e-07	WDR55
WD40_WDHD1_1st	PF24817	433 - 539	1.2e-10	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	457 - 664	2.1e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	477 - 668	4.5e-12	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	824 - 1054	4.2e-10	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	824 - 960	5.8e-13	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	840 - 1039	3.3e-16	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	840 - 962	2e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	841 - 957	1.1e-15	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	841 - 1046	1.1e-14	WDHD1 first WD40 domain
WDR55	PF24796	842 - 956	9.3e-07	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	843 - 955	8.6e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	844 - 1046	1.4e-15	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	847 - 963	1.7e-11	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	859 - 968	1.1e-09	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	860 - 956	7.2e-10	WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	864 - 958	9.6e-08	WDR90/POC16, second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15880 AT3G15880 AT3G15880 AT3G15880
fragaria_vesca	FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730
malus_domestica	MD02G1027700.v1.1 MD11G1043800.v1.1 MD15G1169300.v1.1
prunus_persica	Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1
pyrus_communis	pycom12419g00020
rosa_chinensis	RchiOBHm_Chr2g0087841
rosa_laevigata	RLG00000015906 RLG00000019456
rosa_multiflora	Rmu_sc0008385.1_g000016
rosa_roxburghii	Rroxscaffold_2G00153180
rosa_rugosa	Rorug01G0479000 Rorug01G0479000 Rorug02G0324100 Rorug02G0324100
rosa_samantha	Rh2BG032700
rosa_wichuraiana	Rw2G002610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	736
AasI	GACNNNNNNGTC	1 cut(s)	290
Acc36I	ACCTGC	3 cut(s)	736, 2488, 3371
AccB1I	GGYRCC	3 cut(s)	626, 2167, 3326
AccB7I	CCANNNNNTGG	2 cut(s)	698, 2259
AccI	GTMKAC	3 cut(s)	600, 1530, 1661
AccII	CGCG	1 cut(s)	3233
AciI	CCGC	6 cut(s)	514, 798, 1544, 1795, 3233, 3366
AclWI	GGATC	7 cut(s)	273, 1696, 2125, 2970, 3242, 3255, 3396
AcoI	YGGCCR	2 cut(s)	762, 1239
AcsI	RAATTY	6 cut(s)	59, 1868, 2001, 2011, 2356, 2894
AcuI	CTGAAG	4 cut(s)	23, 2547, 2568, 2885
AdeI	CACNNNGTG	2 cut(s)	701, 1099
AfaI	GTAC	7 cut(s)	119, 242, 1774, 1906, 2053, 2463, 3294
AfiI	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
AflII	CTTAAG	1 cut(s)	3208
AflIII	ACRYGT	2 cut(s)	910, 2809
AjiI	CACGTC	1 cut(s)	2215
AjnI	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
AjuI	GAANNNNNNNTTGG	4 cut(s)	1820, 1823, 1852, 1855
AleI	CACNNNNGTG	1 cut(s)	2470
Alw21I	GWGCWC	2 cut(s)	706, 2400
Alw26I	GTCTC	2 cut(s)	1981, 2304
Alw44I	GTGCAC	1 cut(s)	2396
AlwI	GGATC	7 cut(s)	273, 1696, 2125, 2970, 3242, 3255, 3396
AlwNI	CAGNNNCTG	3 cut(s)	46, 905, 1586
Ama87I	CYCGRG	2 cut(s)	2833, 2943
AoxI	GGCC	9 cut(s)	569, 762, 803, 1239, 1491, 1513, 2094, 2794, 3356
ApaLI	GTGCAC	1 cut(s)	2396
ApoI	RAATTY	6 cut(s)	59, 1868, 2001, 2011, 2356, 2894
Asp700I	GAANNNNTTC	1 cut(s)	1835
AspLEI	GCGC	3 cut(s)	736, 852, 1648
AspS9I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3043, 3356
AsuC2I	CCSGG	1 cut(s)	802
AsuHPI	GGTGA	9 cut(s)	145, 172, 413, 765, 1085, 1148, 1358, 1361, 1862
AvaI	CYCGRG	2 cut(s)	2833, 2943
AvaII	GGWCC	3 cut(s)	710, 1201, 3043
AxyI	CCTNAGG	1 cut(s)	3155
BaeGI	GKGCMC	3 cut(s)	1544, 2400, 3329
BaeI	ACNNNNGTAYC	2 cut(s)	2931, 2964
BalI	TGGCCA	2 cut(s)	764, 1241
BamHI	GGATCC	1 cut(s)	3247
BanI	GGYRCC	3 cut(s)	626, 2167, 3326
BanII	GRGCYC	3 cut(s)	637, 841, 1628
BauI	CACGAG	2 cut(s)	1702, 3048
Bbv12I	GWGCWC	2 cut(s)	706, 2400
BceAI	ACGGC	1 cut(s)	2340
BcgI	CGANNNNNNTGC	6 cut(s)	2100, 2134, 3225, 3259, 3366, 3400
BciT130I	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
BciVI	GTATCC	2 cut(s)	2839, 3228
BcnI	CCSGG	1 cut(s)	802
BcoDI	GTCTC	2 cut(s)	1981, 2304
BfmI	CTRYAG	2 cut(s)	819, 1226
BfoI	RGCGCY	1 cut(s)	853
BfrI	CTTAAG	1 cut(s)	3208
BfuAI	ACCTGC	3 cut(s)	736, 2488, 3371
BfuI	GTATCC	2 cut(s)	2839, 3228
BglI	GCCNNNNNGGC	1 cut(s)	2375
BglII	AGATCT	1 cut(s)	213
BlpI	GCTNAGC	1 cut(s)	11
Bme1390I	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
Bme18I	GGWCC	3 cut(s)	710, 1201, 3043
BmeT110I	CYCGRG	2 cut(s)	2833, 2943
BmgBI	CACGTC	1 cut(s)	2215
BmgT120I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3043, 3356
BmrFI	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
BmrI	ACTGGG	2 cut(s)	1091, 1497
BmsI	GCATC	9 cut(s)	1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
BmuI	ACTGGG	2 cut(s)	1091, 1497
BoxI	GACNNNNGTC	1 cut(s)	1657
BpmI	CTGGAG	2 cut(s)	383, 3393
Bpu1102I	GCTNAGC	1 cut(s)	11
BpuEI	CTTGAG	4 cut(s)	490, 1612, 3017, 3317
BpuMI	CCSGG	1 cut(s)	802
Bsa29I	ATCGAT	2 cut(s)	1259, 1834
BsaBI	GATNNNNATC	1 cut(s)	3252
BsaI	GGTCTC	1 cut(s)	2304
BsaJI	CCNNGG	8 cut(s)	183, 566, 630, 1003, 1037, 1197, 2175, 2832
BsaWI	WCCGGW	1 cut(s)	2164
BsaXI	ACNNNNNCTCC	8 cut(s)	77, 107, 1319, 1349, 3276, 3306, 3319, 3349
Bsc4I	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
Bse118I	RCCGGY	2 cut(s)	1927, 2295
Bse1I	ACTGG	7 cut(s)	545, 965, 980, 1097, 1492, 1852, 3097
Bse21I	CCTNAGG	1 cut(s)	3155
Bse3DI	GCAATG	2 cut(s)	2334, 2697
Bse8I	GATNNNNATC	1 cut(s)	3252
BseBI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
BseCI	ATCGAT	2 cut(s)	1259, 1834
BseDI	CCNNGG	8 cut(s)	183, 566, 630, 1003, 1037, 1197, 2175, 2832
BseJI	GATNNNNATC	1 cut(s)	3252
BseLI	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
BseMI	GCAATG	2 cut(s)	2334, 2697
BseMII	CTCAG	4 cut(s)	2024, 2294, 2861, 3169
BseNI	ACTGG	7 cut(s)	545, 965, 980, 1097, 1492, 1852, 3097
BseRI	GAGGAG	1 cut(s)	852
BseSI	GKGCMC	3 cut(s)	1544, 2400, 3329
BseYI	CCCAGC	4 cut(s)	877, 2098, 2653, 3353
BsgI	GTGCAG	1 cut(s)	1832
Bsh1236I	CGCG	1 cut(s)	3233
BshFI	GGCC	9 cut(s)	571, 764, 805, 1241, 1493, 1515, 2096, 2796, 3358
BshNI	GGYRCC	3 cut(s)	626, 2167, 3326
BshVI	ATCGAT	2 cut(s)	1259, 1834
BsiHKAI	GWGCWC	2 cut(s)	706, 2400
BsiHKCI	CYCGRG	2 cut(s)	2833, 2943
BsiSI	CCGG	4 cut(s)	801, 1928, 2165, 2296
BslFI	GGGAC	5 cut(s)	656, 1214, 1723, 1889, 3029
BslI	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
BsmAI	GTCTC	2 cut(s)	1981, 2304
BsmFI	GGGAC	5 cut(s)	656, 1214, 1723, 1889, 3029
BsmI	GAATGC	2 cut(s)	317, 3032
BsnI	GGCC	9 cut(s)	571, 764, 805, 1241, 1493, 1515, 2096, 2796, 3358
Bso31I	GGTCTC	1 cut(s)	2304
BsoBI	CYCGRG	2 cut(s)	2833, 2943
Bsp1286I	GDGCHC	7 cut(s)	637, 706, 841, 1544, 1628, 2400, 3329
Bsp1720I	GCTNAGC	1 cut(s)	11
Bsp19I	CCATGG	2 cut(s)	1003, 2175
BspACI	CCGC	6 cut(s)	514, 798, 1544, 1795, 3233, 3366
BspANI	GGCC	9 cut(s)	571, 764, 805, 1241, 1493, 1515, 2096, 2796, 3358
BspCNI	CTCAG	4 cut(s)	2025, 2295, 2860, 3168
BspDI	ATCGAT	2 cut(s)	1259, 1834
BspFNI	CGCG	1 cut(s)	3233
BspHI	TCATGA	3 cut(s)	1050, 1143, 2986
BspMAI	CTGCAG	2 cut(s)	823, 1230
BspMI	ACCTGC	3 cut(s)	736, 2488, 3371
BspPI	GGATC	7 cut(s)	273, 1696, 2125, 2970, 3242, 3255, 3396
BspT107I	GGYRCC	3 cut(s)	626, 2167, 3326
BspTI	CTTAAG	1 cut(s)	3208
BspTNI	GGTCTC	1 cut(s)	2304
BsrDI	GCAATG	2 cut(s)	2334, 2697
BsrFI	RCCGGY	2 cut(s)	1927, 2295
BsrI	ACTGG	7 cut(s)	545, 965, 980, 1097, 1492, 1852, 3097
BssAI	RCCGGY	2 cut(s)	1927, 2295
BssECI	CCNNGG	8 cut(s)	183, 566, 630, 1003, 1037, 1197, 2175, 2832
BssNAI	GTATAC	1 cut(s)	1531
BssSI	CACGAG	2 cut(s)	1702, 3048
BssT1I	CCWWGG	4 cut(s)	183, 566, 1003, 2175
Bst1107I	GTATAC	1 cut(s)	1531
Bst2BI	CACGAG	2 cut(s)	1702, 3048
Bst2UI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
Bst4CI	ACNGT	9 cut(s)	387, 1268, 1679, 1801, 1968, 2041, 2134, 2198, 2472
Bst6I	CTCTTC	3 cut(s)	48, 147, 914
BstAFI	CTTAAG	1 cut(s)	3208
BstAPI	GCANNNNNTGC	1 cut(s)	1679
BstDEI	CTNAG	8 cut(s)	11, 393, 1314, 2033, 2303, 2763, 2847, 3155
BstDSI	CCRYGG	2 cut(s)	1003, 2175
BstFNI	CGCG	1 cut(s)	3233
BstH2I	RGCGCY	1 cut(s)	853
BstHHI	GCGC	3 cut(s)	736, 852, 1648
BstMAI	GTCTC	2 cut(s)	1981, 2304
BstNI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
BstNSI	RCATGY	5 cut(s)	914, 1388, 2813, 3077, 3293
BstPAI	GACNNNNGTC	1 cut(s)	1657
BstSCI	CCNGG	7 cut(s)	629, 800, 1037, 1063, 1196, 1648, 2252
BstSFI	CTRYAG	2 cut(s)	819, 1226
BstSLI	GKGCMC	3 cut(s)	1544, 2400, 3329
BstUI	CGCG	1 cut(s)	3233
BstX2I	RGATCY	2 cut(s)	213, 3247
BstXI	CCANNNNNNTGG	5 cut(s)	611, 1004, 2472, 2668, 2710
BstYI	RGATCY	2 cut(s)	213, 3247
BstZ17I	GTATAC	1 cut(s)	1531
Bsu15I	ATCGAT	2 cut(s)	1259, 1834
Bsu36I	CCTNAGG	1 cut(s)	3155
BsuI	GTATCC	2 cut(s)	2839, 3228
BsuRI	GGCC	9 cut(s)	571, 764, 805, 1241, 1493, 1515, 2096, 2796, 3358
BsuTUI	ATCGAT	2 cut(s)	1259, 1834
BtgI	CCRYGG	2 cut(s)	1003, 2175
BtrI	CACGTC	1 cut(s)	2215
BtsI	GCAGTG	2 cut(s)	1399, 3044
BtsIMutI	CAGTG	8 cut(s)	1104, 1224, 1399, 1485, 1684, 2046, 2130, 3044
BveI	ACCTGC	3 cut(s)	736, 2488, 3371
CaiI	CAGNNNCTG	3 cut(s)	46, 905, 1586
CciI	TCATGA	3 cut(s)	1050, 1143, 2986
CfoI	GCGC	3 cut(s)	736, 852, 1648
Cfr10I	RCCGGY	2 cut(s)	1927, 2295
Cfr13I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3043, 3356
ClaI	ATCGAT	2 cut(s)	1259, 1834
Csp6I	GTAC	7 cut(s)	118, 241, 1773, 1905, 2052, 2462, 3293
CspCI	CAANNNNNGTGG	2 cut(s)	261, 296
CviQI	GTAC	7 cut(s)	118, 241, 1773, 1905, 2052, 2462, 3293
DdeI	CTNAG	8 cut(s)	11, 393, 1314, 2033, 2303, 2763, 2847, 3155
DraIII	CACNNNGTG	2 cut(s)	701, 1099
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	2 cut(s)	762, 1239
Eam1104I	CTCTTC	3 cut(s)	48, 147, 914
EarI	CTCTTC	3 cut(s)	48, 147, 914
EciI	GGCGGA	1 cut(s)	787
Eco130I	CCWWGG	4 cut(s)	183, 566, 1003, 2175
Eco147I	AGGCCT	1 cut(s)	571
Eco24I	GRGCYC	3 cut(s)	637, 841, 1628
Eco31I	GGTCTC	1 cut(s)	2304
Eco47I	GGWCC	3 cut(s)	710, 1201, 3043
Eco57I	CTGAAG	4 cut(s)	23, 2547, 2568, 2885
Eco81I	CCTNAGG	1 cut(s)	3155
Eco88I	CYCGRG	2 cut(s)	2833, 2943
EcoO109I	RGGNCCY	3 cut(s)	1201, 2095, 3043
EcoRII	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
EcoT14I	CCWWGG	4 cut(s)	183, 566, 1003, 2175
EcoT38I	GRGCYC	3 cut(s)	637, 841, 1628
ErhI	CCWWGG	4 cut(s)	183, 566, 1003, 2175
FaqI	GGGAC	5 cut(s)	656, 1214, 1723, 1889, 3029
FauI	CCCGC	1 cut(s)	1551
FblI	GTMKAC	3 cut(s)	600, 1530, 1661
FriOI	GRGCYC	3 cut(s)	637, 841, 1628
GlaI	GCGC	3 cut(s)	735, 851, 1647
GsaI	CCCAGC	4 cut(s)	881, 2102, 2657, 3357
GsuI	CTGGAG	2 cut(s)	383, 3393
HaeII	RGCGCY	1 cut(s)	853
HaeIII	GGCC	9 cut(s)	571, 764, 805, 1241, 1493, 1515, 2096, 2796, 3358
HapII	CCGG	4 cut(s)	801, 1928, 2165, 2296
HhaI	GCGC	3 cut(s)	736, 852, 1648
Hin6I	GCGC	3 cut(s)	734, 850, 1646
HinP1I	GCGC	3 cut(s)	734, 850, 1646
HincII	GTYRAC	3 cut(s)	357, 1636, 1891
HindII	GTYRAC	3 cut(s)	357, 1636, 1891
HindIII	AAGCTT	2 cut(s)	249, 3205
HpaI	GTTAAC	1 cut(s)	1891
HpaII	CCGG	4 cut(s)	801, 1928, 2165, 2296
HphI	GGTGA	9 cut(s)	145, 172, 413, 765, 1085, 1148, 1358, 1361, 1862
HpyCH4III	ACNGT	9 cut(s)	387, 1268, 1679, 1801, 1968, 2041, 2134, 2198, 2472
HpyCH4IV	ACGT	2 cut(s)	2214, 2571
HpyF3I	CTNAG	8 cut(s)	11, 393, 1314, 2033, 2303, 2763, 2847, 3155
HpySE526I	ACGT	2 cut(s)	2214, 2571
HspAI	GCGC	3 cut(s)	734, 850, 1646
KflI	GGGWCCC	1 cut(s)	3043
KroI	GCCGGC	1 cut(s)	1927
KroNI	GCCGGC	1 cut(s)	1929
KspAI	GTTAAC	1 cut(s)	1891
LmnI	GCTCC	8 cut(s)	85, 640, 836, 1760, 2108, 2167, 2837, 3374
LweI	GCATC	9 cut(s)	1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
MaeII	ACGT	2 cut(s)	2214, 2571
MaeIII	GTNAC	6 cut(s)	133, 646, 940, 1155, 1441, 1483
MflI	RGATCY	2 cut(s)	213, 3247
MhlI	GDGCHC	7 cut(s)	637, 706, 841, 1544, 1628, 2400, 3329
MlsI	TGGCCA	2 cut(s)	764, 1241
MluNI	TGGCCA	2 cut(s)	764, 1241
MlyI	GAGTC	8 cut(s)	101, 352, 511, 911, 1288, 2056, 2246, 2558
MmeI	TCCRAC	6 cut(s)	63, 764, 1102, 2116, 2161, 3362
Mox20I	TGGCCA	2 cut(s)	764, 1241
MroNI	GCCGGC	1 cut(s)	1927
MroXI	GAANNNNTTC	1 cut(s)	1835
MscI	TGGCCA	2 cut(s)	764, 1241
MslI	CAYNNNNRTG	6 cut(s)	609, 1347, 2290, 2470, 2495, 2708
Msp20I	TGGCCA	2 cut(s)	764, 1241
MspA1I	CMGCKG	4 cut(s)	352, 1073, 2657, 3182
MspCI	CTTAAG	1 cut(s)	3208
MspI	CCGG	4 cut(s)	801, 1928, 2165, 2296
MspR9I	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
Mva1269I	GAATGC	2 cut(s)	317, 3032
MvaI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
MvnI	CGCG	1 cut(s)	3233
NaeI	GCCGGC	1 cut(s)	1929
NciI	CCSGG	1 cut(s)	802
NcoI	CCATGG	2 cut(s)	1003, 2175
NgoMIV	GCCGGC	1 cut(s)	1927
NmuCI	GTSAC	3 cut(s)	133, 1441, 1483
NspI	RCATGY	5 cut(s)	914, 1388, 2813, 3077, 3293
OliI	CACNNNNGTG	1 cut(s)	2470
PagI	TCATGA	3 cut(s)	1050, 1143, 2986
PaqCI	CACCTGC	1 cut(s)	736
PceI	AGGCCT	1 cut(s)	571
PciI	ACATGT	2 cut(s)	910, 2809
PctI	GAATGC	2 cut(s)	317, 3032
PdiI	GCCGGC	1 cut(s)	1929
PdmI	GAANNNNTTC	1 cut(s)	1835
PflMI	CCANNNNNTGG	2 cut(s)	698, 2259
PfoI	TCCNGGA	1 cut(s)	2252
PleI	GAGTC	8 cut(s)	100, 352, 511, 910, 1287, 2055, 2245, 2558
PpsI	GAGTC	8 cut(s)	100, 352, 511, 910, 1287, 2055, 2245, 2558
PpuMI	RGGWCCY	2 cut(s)	1201, 3043
PscI	ACATGT	2 cut(s)	910, 2809
PshAI	GACNNNNGTC	1 cut(s)	1657
Psp5II	RGGWCCY	2 cut(s)	1201, 3043
Psp6I	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
PspFI	CCCAGC	4 cut(s)	877, 2098, 2653, 3353
PspGI	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
PspPI	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3043, 3356
PspPPI	RGGWCCY	2 cut(s)	1201, 3043
PsrI	GAACNNNNNNTAC	2 cut(s)	850, 882
PstI	CTGCAG	2 cut(s)	823, 1230
PstNI	CAGNNNCTG	3 cut(s)	46, 905, 1586
PsuI	RGATCY	2 cut(s)	213, 3247
PvuII	CAGCTG	4 cut(s)	352, 1073, 2657, 3182
RsaI	GTAC	7 cut(s)	119, 242, 1774, 1906, 2053, 2463, 3294
RsaNI	GTAC	7 cut(s)	118, 241, 1773, 1905, 2052, 2462, 3293
RseI	CAYNNNNRTG	6 cut(s)	609, 1347, 2290, 2470, 2495, 2708
Sau96I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3043, 3356
SchI	GAGTC	8 cut(s)	101, 352, 511, 911, 1288, 2056, 2246, 2558
ScrFI	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
SduI	GDGCHC	7 cut(s)	637, 706, 841, 1544, 1628, 2400, 3329
SfaNI	GCATC	9 cut(s)	1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
SfcI	CTRYAG	2 cut(s)	819, 1226
SinI	GGWCC	3 cut(s)	710, 1201, 3043
SmiMI	CAYNNNNRTG	6 cut(s)	609, 1347, 2290, 2470, 2495, 2708
SmlI	CTYRAG	5 cut(s)	505, 1627, 3032, 3208, 3296
SmoI	CTYRAG	5 cut(s)	505, 1627, 3032, 3208, 3296
SseBI	AGGCCT	1 cut(s)	571
SsiI	CCGC	6 cut(s)	514, 798, 1544, 1795, 3233, 3366
SspI	AATATT	2 cut(s)	973, 3174
StuI	AGGCCT	1 cut(s)	571
StyD4I	CCNGG	7 cut(s)	629, 800, 1037, 1063, 1196, 1648, 2252
StyI	CCWWGG	4 cut(s)	183, 566, 1003, 2175
TaaI	ACNGT	9 cut(s)	387, 1268, 1679, 1801, 1968, 2041, 2134, 2198, 2472
TaiI	ACGT	2 cut(s)	2217, 2574
TaqI	TCGA	8 cut(s)	222, 268, 1259, 1577, 1834, 2155, 2200, 2240
TatI	WGTACW	2 cut(s)	1904, 3292
TauI	GCSGC	1 cut(s)	3236
TscAI	CASTG	8 cut(s)	1104, 1224, 1399, 1492, 1684, 2046, 2137, 3044
TseFI	GTSAC	3 cut(s)	133, 1441, 1483
Tsp45I	GTSAC	3 cut(s)	133, 1441, 1483
TspRI	CASTG	8 cut(s)	1104, 1224, 1399, 1492, 1684, 2046, 2137, 3044
Van91I	CCANNNNNTGG	2 cut(s)	698, 2259
Vha464I	CTTAAG	1 cut(s)	3208
VneI	GTGCAC	1 cut(s)	2396
VpaK11BI	GGWCC	3 cut(s)	710, 1201, 3043
XapI	RAATTY	6 cut(s)	59, 1868, 2001, 2011, 2356, 2894
XceI	RCATGY	5 cut(s)	914, 1388, 2813, 3077, 3293
XmiI	GTMKAC	3 cut(s)	600, 1530, 1661
XmnI	GAANNNNTTC	1 cut(s)	1835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.