Prupe.7G247300_v2.0.a1

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001857
Physical Location & Seq
Reverse (-)
21001608 .. 21012150
10543 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G247300.10

Sequence Viewer

Length: 3396 bp
ATGTCCTCGCTTAGCAGAGAGCTCGTGTTTCTCATACTTCAGTTTCTGGATGAAGAAAAATTTAAAGACAGTGTTCACAAGTTGGAGCAAGAGTCGGGGTTTTTCTTTAACATGAGGTATTTCGACGACATGGTGACAAATGGGGAGTGGGAGGAGGTGGAGAAGTATTTGTCCGGTTTCACAAAGGTTGATGATAATAGATACTCTATGAAGATCTTCTTTGAAATTCGCAAGCAGAAGTACCTCGAAGCTTTAGACAAGCGTGATCGAGCAAAAGCTGTGGACATTTTAGTTAAGGACTTGAAAGTGTTTGCAGCATTTAATGAGGAACTTTTTAAGGAAATTACACAGCTGTTGACTTTGGATAACTTCAGGGATAATGAACAGTTATCTAAATATGGTGATACAAAGTCTGCAAGGAGCATAATGCTTGCTGAACTGAAAAAGTTAATAGAGGCGAACCCTTTGTTTCGTGATAAACTTCAATTTCCTACCCTGAAGAACTCGAGATTGCGGACTCTTATTAATCAGAGTTTAAACTGGCAGCATCAGCTTTGTAAGAATCCGAGGCCTAACCCTGACATAAAGACCCTATTCGTAGACCACAGTTGTGGACAACCAAATGGTGCCAGGGCTCCATCCCCTGTCACTAATCACTTAATGGGGGCGGTCCCTAAGACAGGGGGTTTCCCACCATTGGGTGCTCACGGGCCCTTTCAGCCTGCACCAGCTGCTCTTCCAACATCCCTTGCGGGATGGATGGCAAATCCATCACCTGTGCCTCATCCCTCGGCTTCTGCTGGGCCTATTGGTTTGGCCGCAGCTAACAATGCAGCAGCCATGTTAAAGCGGCCTAGAACTCCTCCTACCAATAACCCAACTATGGACTATCAAACAGCAGATTCTGAACACGTGCTGAAGAGATCAAGACCTTTCGGAATAACTGATGAAGCCAATAATCTGCCAGTAAATATGCTGCCTGTTGCGTTTCCTAACCAGAGCCATGGTCAGAGTTCCTACTCTTCTGATGACTTGCCCAGGTCTGTTGTTATGACTTTAAGTCCAGGTTCAGCTGTCAAGAGTATGGATTTCCACCCAGTGCAACAAATTATACTTCTTGTCGGAACAAACATGGGTGATGTCATGATATATGAGCTACCAAGCCATGAAAAGATTGCTATTAAGAACTTCAAAGTTTGGGATCTTGGAGAATGTTCAGTGGCTCTGAAGGCAACTTTGGCCAGTGACTATACAGCATCAATCAACCGCGTGATGTGGAGCCCTGATGGGACACATTTTGGTGTTGCATACTCTAAGCACATCGTGCACATATACTCCTATCAGGGTGGTGATGATATACGAAATCACCTAGAGATCGAGGCTCATGTTGGAAGTGTAAATGATCTCGCTTTCTCATATCCCAACAAACAGCTGTGTGTTGTCACGTGTGGGGAGGATAGGGTCATTAAGGTGTGGGATGCAGTCACTGGGACTAAGCACTATACTTTTGAGGGCCATGAAGCACCTGTATATTCGGTGTGTCCGCATCACAAGGAGAATATCCAGTTTATTTTCTCAACAGCTACTGATGGAAAAATAAAGGCATGGTTGTATGATACTGTGGGCTCAAGAGTTGACTATGATGCACCAGGCCATTCTTCTACCACCATGGCATACAGTGCTGATGGAACAAGGTTGTTCTCATGTGGGACAAATAAAGAAGGTGATTCATACTTGGTGGAGTGGAATGAAAGTGAAGGAGCTGTAAAGCGTACCTATCATGGTCTTGCAAAGCGAACTGTTGGGGTTGTGCAGTTTGATACTACTAAGAATCGGTTCTTGGCGGCTGGTGATGAGTTCACGGTCAAATTTTGGGACATGGACAACGTCAACCCTTTGATAAGTACTGATGCAGATGGTGGATTGCCGGCTTCTCCCGCTATTAGATTTAACAAGGAAGGAATACTGTTAGCTGCATCAACGAATGACAATGGTATTAAAATTCTGGCAAATTCTGATGGAATTAGGTTGCTAAGAACTGTGGAAAGTCGTACATTTGATGCTTCAAGAGCTGCTTCTGCAGCTGCTGTGAAGGCCCCGCCACTAGGAACATTTGGGTCTTCCAGCATAGTTGGAACAAGCATTGGAGAGCGAGCTGCTCCAGTGGTAGCTATGGTTGGACTGAACAGTGATAGTCGAAGTTTGGTTGACGTGAAACCTAGAATTGCCGATGAGTCGGCAGAAAAATCCAGGATATGGAAACTGACTGAAATCAATGAACCATCACAATGCCGGTCACTGAGGCTCCCTGATAGTTTAACAGCAACGAGGGTTTCTAGATTAATTTATACAAATTCAGGACTTGCCGTATTGGCATTATCATCTAATGCTGTACACAAGCTCTGGAAATGGCAAAGAAACGAACGAAACACGACTAAGGCCACAGCTAGTACTGTACCCCAATTGTGGCAACCTGCAAGTGGAATATTGATGACCAATGATATAAGCGATACAAACCCTGAAGATGCTGTTCCATGCTTTGCACTTTCAAAGAATGACTCATATGTTATGTCAGCTTCAGGGGGGAAAATATCCCTTTTCAATATGATGACATTTAAGACAATGACAACATTCATGCCCCCACCACCAGCTGCAACATATCTGGCGTTTCATCCTCAAGACAATAATATCATTGCCATAGGAATGGAAGATTCTTCCATCCAAATTTACAATGTTCGGGTTGATGAGGTTAAAACCAAGCTAAAGGGACATCAAAAAAGAATAACAGGCCTTGCCTTTTCTCATGTTCTTAATGTGCTTGTATCATCGGGCGCTGATTCCCAGTTGTGTGTTTGGAACACAGATGGATGGGAGAAGCAGGCCAGTAAATTTCTTCAGATTCCAAGCGGGCGAGCTGCTGCTCCTCTTGCAGATACCCGAGTTCAGTTTCACTTAGATCAGACACTTTTGCTGGCAGTTCATGAAACACAGATAGCCATATACGAAGCACCAAAATTAGAATGCCTCAAGCAGTGGGTTCCTCGAGAAGCTAGTGGTCCAATCACACATGCTACGTATTCCTGTGATAGCCAGTCAATTTATGTTAGCTTTGAAGATGGAAGTGTGGGGGTTCTCACTGCTTCTACACTTAGATTGAGATGCCGAATACTACCTACTGCTTATTTACCTCCAAATCCAAGCTTTGTGCATCCTCTCGTCGTTGCGGCACATCCATCTGAACCCAATCAGTTTGCATTAGGACTTACAGATGGTGGAGTGCATGTACTTGAGCCTTTAGAATCAGAAGGAAGATGGGGCACTTCCCCTCCGATTGAGAATGGTGCTGGGCCGAGCACCACTTCTGGAGCAGCCGGTTCAGATCAACCCCAAAGGTGA

Protein Analysis

1132

Amino Acids

124.2

Weight (kDa)

6.47

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2482
AccB1I GGYRCC 1 cut(s) 626
AccB7I CCANNNNNTGG 1 cut(s) 2256
AccI GTMKAC 1 cut(s) 600
AccII CGCG 1 cut(s) 1269
AclWI GGATC 1 cut(s) 1209
AcoI YGGCCR 2 cut(s) 816, 1239
AcsI RAATTY 8 cut(s) 59, 225, 1868, 2001, 2011, 2353, 2724, 2888
AcuI CTGAAG 8 cut(s) 23, 355, 518, 938, 1247, 2541, 2562, 2879
AcvI CACGTG 2 cut(s) 913, 1446
AdeI CACNNNGTG 2 cut(s) 1099, 1324
AfaI GTAC 8 cut(s) 242, 1774, 1906, 2053, 2394, 2452, 2457, 3285
AfiI CCNNNNNNNGG 8 cut(s) 680, 697, 698, 883, 2105, 2256, 2466, 2480
AflIII ACRYGT 2 cut(s) 910, 1445
AgsI TTSAA 8 cut(s) 224, 304, 485, 1192, 2067, 2550, 2602, 3113
AhdI GACNNNNNGTC 1 cut(s) 1059
AjiI CACGTC 1 cut(s) 2212
AjnI CCWGG 5 cut(s) 629, 1037, 1063, 1648, 2249
AjuI GAANNNNNNNTTGG 4 cut(s) 1220, 1252, 1823, 1855
AleI CACNNNNGTG 1 cut(s) 609
Alw21I GWGCWC 4 cut(s) 24, 706, 1329, 3356
Alw44I GTGCAC 1 cut(s) 1325
AlwI GGATC 1 cut(s) 1209
AlwNI CAGNNNCTG 5 cut(s) 46, 905, 1487, 1586, 2087
Ama87I CYCGRG 3 cut(s) 505, 2937, 3042
ApaI GGGCCC 1 cut(s) 714
ApaLI GTGCAC 1 cut(s) 1325
ApoI RAATTY 8 cut(s) 59, 225, 1868, 2001, 2011, 2353, 2724, 2888
AseI ATTAAT 2 cut(s) 525, 2342
Asp700I GAANNNNTTC 2 cut(s) 215, 1835
AspLEI GCGC 1 cut(s) 2834
AspS9I GGNCC 8 cut(s) 670, 710, 711, 803, 1513, 2095, 3056, 3347
AsuHPI GGTGA 8 cut(s) 145, 413, 765, 1148, 1358, 1361, 1736, 1862
AvaI CYCGRG 3 cut(s) 505, 2937, 3042
AvaII GGWCC 2 cut(s) 670, 3056
BaeGI GKGCMC 3 cut(s) 714, 1329, 3320
BaeI ACNNNNGTAYC 2 cut(s) 2925, 2958
BalI TGGCCA 1 cut(s) 1241
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 5 cut(s) 24, 637, 714, 1283, 1628
BauI CACGAG 1 cut(s) 23
BbrPI CACGTG 2 cut(s) 913, 1446
BbsI GAAGAC 1 cut(s) 2112
Bbv12I GWGCWC 4 cut(s) 24, 706, 1329, 3356
BceAI ACGGC 1 cut(s) 2351
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 5 cut(s) 631, 1039, 1065, 1650, 2251
BfaI CTAG 7 cut(s) 855, 1370, 2105, 2220, 2337, 2448, 3051
BfmI CTRYAG 1 cut(s) 2079
BfoI RGCGCY 1 cut(s) 2835
BfuAI ACCTGC 1 cut(s) 2482
BglI GCCNNNNNGGC 1 cut(s) 2372
BglII AGATCT 1 cut(s) 213
BlpI GCTNAGC 1 cut(s) 11
BmcAI AGTACT 2 cut(s) 1906, 2452
Bme1390I CCNGG 5 cut(s) 631, 1039, 1065, 1650, 2251
Bme18I GGWCC 2 cut(s) 670, 3056
BmeRI GACNNNNNGTC 1 cut(s) 1059
BmeT110I CYCGRG 3 cut(s) 505, 2937, 3042
BmgBI CACGTC 1 cut(s) 2212
BmgT120I GGNCC 8 cut(s) 670, 710, 711, 803, 1513, 2095, 3056, 3347
BmiI GGNNCC 8 cut(s) 628, 636, 672, 712, 1280, 2097, 2306, 3039
BmrFI CCNGG 5 cut(s) 631, 1039, 1065, 1650, 2251
BmrI ACTGGG 3 cut(s) 1091, 1497, 2836
BmuI ACTGGG 3 cut(s) 1091, 1497, 2836
BpiI GAAGAC 1 cut(s) 2112
BpmI CTGGAG 2 cut(s) 2145, 3384
Bpu1102I GCTNAGC 1 cut(s) 11
BpuEI CTTGAG 4 cut(s) 1612, 2661, 3011, 3308
BsaAI YACGTR 3 cut(s) 913, 1446, 3075
BsaJI CCNNGG 6 cut(s) 566, 630, 789, 1003, 1037, 1668
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 8 cut(s) 680, 697, 698, 883, 2105, 2256, 2466, 2480
Bse118I RCCGGY 3 cut(s) 1927, 2292, 3371
Bse3DI GCAATG 1 cut(s) 2691
BseBI CCWGG 5 cut(s) 631, 1039, 1065, 1650, 2251
BseDI CCNNGG 6 cut(s) 566, 630, 789, 1003, 1037, 1668
BseLI CCNNNNNNNGG 8 cut(s) 680, 697, 698, 883, 2105, 2256, 2466, 2480
BseMI GCAATG 1 cut(s) 2691
BseMII CTCAG 1 cut(s) 2291
BseRI GAGGAG 3 cut(s) 167, 852, 2913
BseSI GKGCMC 3 cut(s) 714, 1329, 3320
BseYI CCCAGC 2 cut(s) 800, 3344
BsgI GTGCAG 2 cut(s) 708, 1832
Bsh1236I CGCG 1 cut(s) 1269
BshNI GGYRCC 1 cut(s) 626
BsiHKAI GWGCWC 4 cut(s) 24, 706, 1329, 3356
BsiHKCI CYCGRG 3 cut(s) 505, 2937, 3042
BsiSI CCGG 4 cut(s) 174, 1928, 2293, 3372
BslFI GGGAC 6 cut(s) 656, 1303, 1504, 1723, 1889, 2781
BslI CCNNNNNNNGG 8 cut(s) 680, 697, 698, 883, 2105, 2256, 2466, 2480
BsmFI GGGAC 6 cut(s) 656, 1303, 1504, 1723, 1889, 2781
BsmI GAATGC 1 cut(s) 3026
BsoBI CYCGRG 3 cut(s) 505, 2937, 3042
Bsp120I GGGCCC 1 cut(s) 710
Bsp1286I GDGCHC 9 cut(s) 24, 637, 706, 714, 1283, 1329, 1628, 3320, 3356
Bsp1407I TGTACA 1 cut(s) 2392
Bsp143I GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
Bsp1720I GCTNAGC 1 cut(s) 11
Bsp19I CCATGG 2 cut(s) 1003, 1668
BspCNI CTCAG 1 cut(s) 2292
BspFNI CGCG 1 cut(s) 1269
BspHI TCATGA 2 cut(s) 1143, 2980
BspLI GGNNCC 8 cut(s) 628, 636, 672, 712, 1280, 2097, 2306, 3039
BspMAI CTGCAG 1 cut(s) 2083
BspMI ACCTGC 1 cut(s) 2482
BspPI GGATC 1 cut(s) 1209
BspQI GCTCTTC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 2691
BsrFI RCCGGY 3 cut(s) 1927, 2292, 3371
BsrGI TGTACA 1 cut(s) 2392
BssAI RCCGGY 3 cut(s) 1927, 2292, 3371
BssECI CCNNGG 6 cut(s) 566, 630, 789, 1003, 1037, 1668
BssMI GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 1003, 1668
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 5 cut(s) 631, 1039, 1065, 1650, 2251
Bst6I CTCTTC 3 cut(s) 741, 914, 1027
BstAPI GCANNNNNTGC 3 cut(s) 731, 1324, 1679
BstAUI TGTACA 1 cut(s) 2392
BstBAI YACGTR 3 cut(s) 913, 1446, 3075
BstC8I GCNNGC 9 cut(s) 233, 432, 723, 1929, 2154, 2880, 2909, 2913, 2973
BstDSI CCRYGG 2 cut(s) 1003, 1668
BstENI CCTNNNNNAGG 1 cut(s) 678
BstFNI CGCG 1 cut(s) 1269
BstH2I RGCGCY 1 cut(s) 2835
BstHHI GCGC 1 cut(s) 2834
BstKTI GATC 8 cut(s) 216, 268, 926, 1204, 1377, 1405, 2959, 3382
BstMBI GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
BstNI CCWGG 5 cut(s) 631, 1039, 1065, 1650, 2251
BstNSI RCATGY 2 cut(s) 3071, 3284
BstSCI CCNGG 5 cut(s) 629, 1037, 1063, 1648, 2249
BstSFI CTRYAG 1 cut(s) 2079
BstSLI GKGCMC 3 cut(s) 714, 1329, 3320
BstSNI TACGTA 1 cut(s) 3075
BstUI CGCG 1 cut(s) 1269
BstV2I GAAGAC 1 cut(s) 2112
BstX2I RGATCY 2 cut(s) 213, 1201
BstXI CCANNNNNNTGG 3 cut(s) 611, 1004, 2704
BstYI RGATCY 2 cut(s) 213, 1201
BtgI CCRYGG 2 cut(s) 1003, 1668
BtrI CACGTC 1 cut(s) 2212
BtsI GCAGTG 2 cut(s) 3038, 3135
BveI ACCTGC 1 cut(s) 2482
Cac8I GCNNGC 9 cut(s) 233, 432, 723, 1929, 2154, 2880, 2909, 2913, 2973
CaiI CAGNNNCTG 5 cut(s) 46, 905, 1487, 1586, 2087
CciI TCATGA 2 cut(s) 1143, 2980
CfoI GCGC 1 cut(s) 2834
Cfr10I RCCGGY 3 cut(s) 1927, 2292, 3371
Cfr13I GGNCC 8 cut(s) 670, 710, 711, 803, 1513, 2095, 3056, 3347
Csp6I GTAC 8 cut(s) 241, 1773, 1905, 2052, 2393, 2451, 2456, 3284
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviQI GTAC 8 cut(s) 241, 1773, 1905, 2052, 2393, 2451, 2456, 3284
DpnI GATC 8 cut(s) 215, 267, 925, 1203, 1376, 1404, 2958, 3381
DpnII GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
DraI TTTAAA 2 cut(s) 64, 537
DraIII CACNNNGTG 2 cut(s) 1099, 1324
DriI GACNNNNNGTC 1 cut(s) 1059
EaeI YGGCCR 2 cut(s) 816, 1239
Eam1104I CTCTTC 3 cut(s) 741, 914, 1027
Eam1105I GACNNNNNGTC 1 cut(s) 1059
EarI CTCTTC 3 cut(s) 741, 914, 1027
Ecl136II GAGCTC 1 cut(s) 22
Eco105I TACGTA 1 cut(s) 3075
Eco130I CCWWGG 2 cut(s) 1003, 1668
Eco147I AGGCCT 2 cut(s) 571, 2790
Eco24I GRGCYC 5 cut(s) 24, 637, 714, 1283, 1628
Eco47I GGWCC 2 cut(s) 670, 3056
Eco53kI GAGCTC 1 cut(s) 22
Eco57I CTGAAG 8 cut(s) 23, 355, 518, 938, 1247, 2541, 2562, 2879
Eco72I CACGTG 2 cut(s) 913, 1446
Eco88I CYCGRG 3 cut(s) 505, 2937, 3042
EcoICRI GAGCTC 1 cut(s) 22
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 2 cut(s) 711, 2095
EcoRII CCWGG 5 cut(s) 629, 1037, 1063, 1648, 2249
EcoT14I CCWWGG 2 cut(s) 1003, 1668
EcoT38I GRGCYC 5 cut(s) 24, 637, 714, 1283, 1628
ErhI CCWWGG 2 cut(s) 1003, 1668
FalI AAGNNNNNCTT 4 cut(s) 203, 235, 2059, 2091
FaqI GGGAC 6 cut(s) 656, 1303, 1504, 1723, 1889, 2781
FauI CCCGC 4 cut(s) 745, 1945, 2106, 2900
FauNDI CATATG 1 cut(s) 2563
FblI GTMKAC 1 cut(s) 600
FriOI GRGCYC 5 cut(s) 24, 637, 714, 1283, 1628
FspBI CTAG 7 cut(s) 855, 1370, 2105, 2220, 2337, 2448, 3051
GlaI GCGC 1 cut(s) 2833
GsaI CCCAGC 2 cut(s) 804, 3348
GsuI CTGGAG 2 cut(s) 2145, 3384
HaeII RGCGCY 1 cut(s) 2835
HapII CCGG 4 cut(s) 174, 1928, 2293, 3372
HhaI GCGC 1 cut(s) 2834
Hin6I GCGC 1 cut(s) 2832
HinP1I GCGC 1 cut(s) 2832
HincII GTYRAC 4 cut(s) 357, 1636, 1891, 2209
HindII GTYRAC 4 cut(s) 357, 1636, 1891, 2209
HindIII AAGCTT 2 cut(s) 249, 3199
HpaII CCGG 4 cut(s) 174, 1928, 2293, 3372
HphI GGTGA 8 cut(s) 145, 413, 765, 1148, 1358, 1361, 1736, 1862
Hpy99I CGWCG 2 cut(s) 128, 3221
HpyAV CCTTC 6 cut(s) 1222, 1715, 1751, 1952, 2086, 3299
HpyCH4IV ACGT 5 cut(s) 912, 1445, 1887, 2211, 3074
HpySE526I ACGT 5 cut(s) 912, 1445, 1887, 2211, 3074
HspAI GCGC 1 cut(s) 2832
KroI GCCGGC 1 cut(s) 1927
KroNI GCCGGC 1 cut(s) 1929
Kzo9I GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 9 cut(s) 85, 420, 640, 1278, 1760, 2164, 2310, 2926, 3365
MaeI CTAG 7 cut(s) 855, 1370, 2105, 2220, 2337, 2448, 3051
MaeII ACGT 5 cut(s) 912, 1445, 1887, 2211, 3074
MaeIII GTNAC 6 cut(s) 133, 646, 1244, 1441, 1483, 2295
MalI GATC 8 cut(s) 215, 267, 925, 1203, 1376, 1404, 2958, 3381
MboI GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
MfeI CAATTG 1 cut(s) 2462
MflI RGATCY 2 cut(s) 213, 1201
MhlI GDGCHC 9 cut(s) 24, 637, 706, 714, 1283, 1329, 1628, 3320, 3356
MlsI TGGCCA 1 cut(s) 1241
MluNI TGGCCA 1 cut(s) 1241
MlyI GAGTC 4 cut(s) 101, 511, 2243, 2552
MmeI TCCRAC 6 cut(s) 63, 764, 1102, 1369, 2113, 2158
Mox20I TGGCCA 1 cut(s) 1241
MroNI GCCGGC 1 cut(s) 1927
MroXI GAANNNNTTC 2 cut(s) 215, 1835
MscI TGGCCA 1 cut(s) 1241
MslI CAYNNNNRTG 5 cut(s) 609, 1299, 1469, 2287, 2702
Msp20I TGGCCA 1 cut(s) 1241
MspA1I CMGCKG 6 cut(s) 352, 731, 1073, 1432, 2084, 2651
MspI CCGG 4 cut(s) 174, 1928, 2293, 3372
MspR9I CCNGG 5 cut(s) 631, 1039, 1065, 1650, 2251
MssI GTTTAAAC 1 cut(s) 537
MunI CAATTG 1 cut(s) 2462
Mva1269I GAATGC 1 cut(s) 3026
MvaI CCWGG 5 cut(s) 631, 1039, 1065, 1650, 2251
MvnI CGCG 1 cut(s) 1269
NaeI GCCGGC 1 cut(s) 1929
NcoI CCATGG 2 cut(s) 1003, 1668
NdeI CATATG 1 cut(s) 2563
NdeII GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
NgoMIV GCCGGC 1 cut(s) 1927
NlaIV GGNNCC 8 cut(s) 628, 636, 672, 712, 1280, 2097, 2306, 3039
NmeAIII GCCGAG 2 cut(s) 770, 3375
NmuCI GTSAC 6 cut(s) 133, 646, 1244, 1441, 1483, 2295
NspI RCATGY 2 cut(s) 3071, 3284
OliI CACNNNNGTG 1 cut(s) 609
PaeR7I CTCGAG 2 cut(s) 505, 3042
PagI TCATGA 2 cut(s) 1143, 2980
PceI AGGCCT 2 cut(s) 571, 2790
PciSI GCTCTTC 1 cut(s) 741
PctI GAATGC 1 cut(s) 3026
PdiI GCCGGC 1 cut(s) 1929
PdmI GAANNNNTTC 2 cut(s) 215, 1835
PfeI GAWTC 8 cut(s) 562, 902, 1727, 1831, 2711, 2837, 2899, 3299
PflFI GACNNNGTC 1 cut(s) 1886
PflMI CCANNNNNTGG 1 cut(s) 2256
PfoI TCCNGGA 1 cut(s) 2249
PleI GAGTC 4 cut(s) 100, 511, 2242, 2552
PmaCI CACGTG 2 cut(s) 913, 1446
PmeI GTTTAAAC 1 cut(s) 537
PmlI CACGTG 2 cut(s) 913, 1446
PpsI GAGTC 4 cut(s) 100, 511, 2242, 2552
Ppu21I YACGTR 3 cut(s) 913, 1446, 3075
PshBI ATTAAT 2 cut(s) 525, 2342
Psp124BI GAGCTC 1 cut(s) 24
Psp6I CCWGG 5 cut(s) 629, 1037, 1063, 1648, 2249
PspCI CACGTG 2 cut(s) 913, 1446
PspFI CCCAGC 2 cut(s) 800, 3344
PspGI CCWGG 5 cut(s) 629, 1037, 1063, 1648, 2249
PspN4I GGNNCC 8 cut(s) 628, 636, 672, 712, 1280, 2097, 2306, 3039
PspOMI GGGCCC 1 cut(s) 710
PspPI GGNCC 8 cut(s) 670, 710, 711, 803, 1513, 2095, 3056, 3347
PsrI GAACNNNNNNTAC 2 cut(s) 850, 882
PstI CTGCAG 1 cut(s) 2083
PstNI CAGNNNCTG 5 cut(s) 46, 905, 1487, 1586, 2087
PsuI RGATCY 2 cut(s) 213, 1201
PsyI GACNNNGTC 1 cut(s) 1886
PvuII CAGCTG 6 cut(s) 352, 731, 1073, 1432, 2084, 2651
RsaI GTAC 8 cut(s) 242, 1774, 1906, 2053, 2394, 2452, 2457, 3285
RsaNI GTAC 8 cut(s) 241, 1773, 1905, 2052, 2393, 2451, 2456, 3284
RseI CAYNNNNRTG 5 cut(s) 609, 1299, 1469, 2287, 2702
SacI GAGCTC 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 741
Sau3AI GATC 8 cut(s) 213, 265, 923, 1201, 1374, 1402, 2956, 3379
Sau96I GGNCC 8 cut(s) 670, 710, 711, 803, 1513, 2095, 3056, 3347
ScaI AGTACT 2 cut(s) 1906, 2452
SchI GAGTC 4 cut(s) 101, 511, 2243, 2552
ScrFI CCNGG 5 cut(s) 631, 1039, 1065, 1650, 2251
SduI GDGCHC 9 cut(s) 24, 637, 706, 714, 1283, 1329, 1628, 3320, 3356
SfcI CTRYAG 1 cut(s) 2079
Sfr274I CTCGAG 2 cut(s) 505, 3042
SinI GGWCC 2 cut(s) 670, 3056
SlaI CTCGAG 2 cut(s) 505, 3042
SmiMI CAYNNNNRTG 5 cut(s) 609, 1299, 1469, 2287, 2702
SmlI CTYRAG 6 cut(s) 505, 1627, 2676, 3026, 3042, 3287
SmoI CTYRAG 6 cut(s) 505, 1627, 2676, 3026, 3042, 3287
SnaBI TACGTA 1 cut(s) 3075
SseBI AGGCCT 2 cut(s) 571, 2790
SspI AATATT 1 cut(s) 2487
SspMI CTAG 7 cut(s) 855, 1370, 2105, 2220, 2337, 2448, 3051
SstI GAGCTC 1 cut(s) 24
StuI AGGCCT 2 cut(s) 571, 2790
StyD4I CCNGG 5 cut(s) 629, 1037, 1063, 1648, 2249
StyI CCWWGG 2 cut(s) 1003, 1668
TaiI ACGT 5 cut(s) 915, 1448, 1890, 2214, 3077
TaqI TCGA 7 cut(s) 123, 246, 268, 506, 1377, 2197, 3043
TatI WGTACW 4 cut(s) 1904, 2392, 2450, 3283
TauI GCSGC 4 cut(s) 821, 853, 1847, 3227
TfiI GAWTC 8 cut(s) 562, 902, 1727, 1831, 2711, 2837, 2899, 3299
TseFI GTSAC 6 cut(s) 133, 646, 1244, 1441, 1483, 2295
Tsp45I GTSAC 6 cut(s) 133, 646, 1244, 1441, 1483, 2295
Tth111I GACNNNGTC 1 cut(s) 1886
Van91I CCANNNNNTGG 1 cut(s) 2256
VneI GTGCAC 1 cut(s) 1325
VpaK11BI GGWCC 2 cut(s) 670, 3056
VspI ATTAAT 2 cut(s) 525, 2342
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 8 cut(s) 59, 225, 1868, 2001, 2011, 2353, 2724, 2888
XbaI TCTAGA 1 cut(s) 2336
XceI RCATGY 2 cut(s) 3071, 3284
XhoI CTCGAG 2 cut(s) 505, 3042
XmiI GTMKAC 1 cut(s) 600
XmnI GAANNNNTTC 2 cut(s) 215, 1835
XspI CTAG 7 cut(s) 855, 1370, 2105, 2220, 2337, 2448, 3051
ZrmI AGTACT 2 cut(s) 1906, 2452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.