Rw2G002610

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001857
Physical Location & Seq
Forward (+)
2300610 .. 2308892
8283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002610.1

Sequence Viewer

Length: 3444 bp
ATGTCCTCGCTGAGCAGAGAGCTTGTGTTTCTCATACTTCAGTTTCTGGATGAAGAGAAATTCAAAGAGAGTGTTCACAAGTTGGAGCAAGAGTCAGGGTTTTTCTTTAACATGAGGTACTTTGAAGATATGGTGACAAATGGGGAGTGGGAAGAGGTGGAGAAGTATTTGTCTGGTTTCACCAAGGTTGATGATAATAGATACTCTATGAAGATCTTTTTTGAGATACGCAAGCAGAAGTACCTTGAAGCTTTAGACAAGAGGGATCGAGCAAAAGCTGTGGACATTTTAGTCAAGGACTTGAAAGTGTTTGCAGCATTCAATGAAGAACTTTTTAAGGAAATTACACAGCTGTTGACTCTGGATAACTTTAGGGATAATGAACAGTTATCTAAGTATGGTGATACAAAGTCTGCAAGGGGTATAATGCTTGCTGAACTGAAAAAGTTAATAGAGGCTAACCCATTGTTTCGTGATAAACTTCAATTTCCTACCCTGAAAAACTCAAGATTGCGGACTCTTATCAATCAGAGCTTAAACTGGCAACACCAGCTTTGTAAGAATCCAAGGCCTAACCCTGACATAAAGACCCTATTTGTAGACCATAGCTGTGGACAACCGAATGGTGCCAGGGCTCCATCCCCTGTTACTAATCATTTAATGGGGGCAGTCCCAAAGACGGGGGGTTTCCCTCCACTTGGTGCTCATGGACCCTTTCAGCCCACACCTGCTGCGCTTCCAACATCTCTTGCTGGATGGATGGCCAACTCATCACCTGTGCCTCATCCCTCTGCTTCCGCCGGGCCGATGGGTTTGGCTGCAGCTAACAATGCAGGAGCCCTCTTAAAGCGCCCTAGAACTCCTCCTACCAATAACCCGGCTATGGACTATCAAACAGCAGATTCTGAACATGTGATGAAGAGATCAAGACCTTTTGGAGTAACAGATGAGGCAAATAATCTGCCAGTAAATATTCTGCCAGTTGCTTATCCTAACCAGAGCCATGGTCAGAGTTCCTACTCTACTGATGACTTGCCCAGGAATCTAGTCATGACAATAAATCCAGGTTCAGCTGTCAAGAGTATGGATTTCCACCCAGTGCAACAGATTCTACTACTTGTTGGAACAAATATGGGTGACGTCATGGTATATGAGTTACCAAGCCATGAAAGAATTGCTATTAGGAACTTCAAAGCCTGGGACCTTGCAACATGTTCAGTGGCTCTGCAGGCAACTTTGGCCAATGATTATACAGCATCGATCAACCGTGTAGTGTGGAGTCCTGATGGAACTCTTTTTGGTGTTGCATACTCTAAGCATCTAGTTCACATATACTCCTATCATGGTGGTGATGATTTAAGAAATCATCTAGAGATTGAGGCACATACTGGCAGTGTTAATGATCTTGCTTTCTCATATCCAAACAAACAGCTCTGTGTTGTGACTTGTGGCGAGGATAGGGTTATCAAGGTGTGGGATGCTGTCACTGGGGCTAAGCAGCATACATTTGAGGGCCATGAAGCACCTGTATACTCGGTGTGCCCGCATCACAAGGAGAGTATTCAGTTTATTTTCTCGACAGCAACTGATGGAAAAATAAAGGCATGGCTGTATGATAATATGGGCTCAAGAGTTGACTATGATGCGCCAGGACATTCGTCTACCACTATGGCGTACAGTGCTGATGGATCAAGGCTATTCTCGTGTGGGACAAACAAAGAAGGGGATTCATACTTGGTGGAATGGAATGAAAGTGAAGGAGCTGTAAAGCGTACCTATCATGGTCTTGGGAAGCGGACTGTTGGGGTTGTGCAGTTTGATACCACCAAGAATCGATTCTTGGCTGCTGGTGATGAGTTCATGGTTAAATTCTGGGACATGGACAGTGTTAACCTTTTGACAAGTACAGATGCAGATGGTGGACTGCCGGCTTCTCCTTCTATTAGATTTAACAAAGAAGGAATACTGTTAGCTGTCTCAACAAATGACAATGGTATTAAAATTCTAGCAAATTCAGATGGAATTAGGTTGCTGAGAACAGTGGAGAGTCGTACATTTGATGCTTCCAGAGCTGCTTCTGTGGCTATTGTGAAGGCCCCAGCAATAGGAGCATTTGGATCGTCTAACATTACTGTTGGAACAGGCATTGGAGATCGAGCAGCTCCGGTGCCAGCCATGGTTGGACTGAACAATGACAGTCGAAGTTTGGCTGACGTGAAACCTAGAATTGTTGATGAGTCGATAGAGAAATCCAGGATCTGGAAACTGACTGAAATCAATGAACCATCACAATGCCGGTCTCTGAGGCTTCCTGATAATTTAACAGCAATGAGGGTGTCTAGGTTAATTTACACAAATTCAGGACTTGCCATATTGGCATTATCATCTAATGCTGTGCACAAGCTCTGGAAATGGCAACGAAATGACAGAACCACGATAGGGAAGGCCACAGCTAGTATTGTACCACAACTGTGGCAACCTGCAAGTGGCATACTAATGACTAATGATATAAGTGATACAAACCCTGAAGATGCTGTTCCATGCTTTGCACTTTCAAAGAATGACTCTTACGTTATGTCAGCTTCAGGGGGGAAAATATCCCTGTTCAATATGATGACATTTAAGACGATGACAACATTCATGCCTCCACCCCCAGCTGCCACATATCTGGCATTTCATCCCCAAGACAATAATATCATTGCCATAGGCATGGAAGATTCTTCCATCCAGATTTACAATGTTCGGGTTGATGAGGTTAAAACTAAGCTAAAAGGTCATCAGAAAAGAATAACAGGCCTTGCCTTCTCACATGTTCTTAATGTGCTTGTATCCTCGGGAGCTGATTCTCAGTTGTGTGTTTGGAACACAGATGGATGGGAGAGGCAAGCTAGTAAATTCCTTCAGATTCCAAGTGGGCGAGCTTCTGCCCCTCTTGCAGATACCCGGGTTCAGTTCCACTTGGATCAGATACACTTGCTGGCAGTTCATGAAACACAGATAGCCATTTACGAAGCACCAAAATTAGAATGCCTCAAGCAGTGGGTCCCACCAGAGGCTAGTGGTCCAATCACACATGCTACTTATTCCTGTGATAGCCAGTCAATTTATGTTAGCTCTGCAGATGGAAGTGTGGGGGTGCTTACTGCTACAACCCTCAGGTTGAGATGCCGAATATTACCAGCTGCTTATTTACCTCCAAATCCAAGCTTAAGCGTGTATCCTCTTGTTGTCGCGGCACATCCATCGGATCCCAATCAGTTTGCACTAGGTCTTACAGATGGTGGAGTGCATGTACTTGAGCCATTAGAATCAGCAGGAAAATGGGGCACCTCCCCTCCAACTGAAAATGGTGCTGGGCCTAGCACCGCTTCTGGAGCAGCAGGTTCGGATCAACCTCAAAGTTTCTTTGATTCGCGGCAACTGTTCATTTTTTTTTTTGTCTAA

Protein Analysis

1147

Amino Acids

125.95

Weight (kDa)

6.47

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 1.6e-12 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 7e-64 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 1e-24 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 347 - 474 3.5e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 359 - 573 7.8e-11 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 363 - 512 1.1e-07 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 395 - 573 6.7e-17 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 401 - 572 2.1e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 417 - 629 1.3e-19 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 419 - 677 4.2e-25 THOC3 beta-propeller domain
WDR55 PF24796 419 - 536 3.7e-07 WDR55
WD40_Gbeta PF25391 424 - 627 9.4e-06 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 433 - 539 3.6e-11 WDHD1 first WD40 domain
Beta-prop_EML PF23409 457 - 665 2e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 459 - 493 9.4e-06 WD domain, G-beta repeat
WD40_Prp19 PF24814 477 - 668 5.4e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 824 - 960 6.9e-13 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 824 - 1056 1.2e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 840 - 1041 6.9e-17 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 840 - 962 2e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 841 - 957 1.1e-15 WDR3 first beta-propeller domain
WDR55 PF24796 842 - 956 9.5e-07 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 843 - 955 9.1e-09 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 843 - 1046 8.6e-16 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 844 - 1047 5.2e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 847 - 964 1.3e-11 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 859 - 966 1.2e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 860 - 956 7.3e-10 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 865 - 958 1e-07 WDR90/POC16, second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 736
AasI GACNNNNNNGTC 1 cut(s) 290
AatII GACGTC 1 cut(s) 1143
Acc36I ACCTGC 3 cut(s) 736, 2488, 3371
AccB1I GGYRCC 3 cut(s) 626, 2167, 3326
AccB7I CCANNNNNTGG 1 cut(s) 2259
AccI GTMKAC 3 cut(s) 600, 1530, 1661
AccII CGCG 2 cut(s) 3233, 3415
AciI CCGC 7 cut(s) 514, 798, 1544, 1795, 3233, 3366, 3415
AclWI GGATC 8 cut(s) 273, 1696, 2125, 2264, 2970, 3242, 3255, 3396
AcoI YGGCCR 2 cut(s) 762, 1239
AcsI RAATTY 6 cut(s) 59, 1868, 2001, 2011, 2356, 2894
AcuI CTGAAG 4 cut(s) 23, 2547, 2568, 2885
AcyI GRCGYC 1 cut(s) 1140
AdeI CACNNNGTG 2 cut(s) 701, 1099
AfaI GTAC 8 cut(s) 119, 242, 1676, 1774, 1906, 2053, 2463, 3294
AfiI CCNNNNNNNGG 9 cut(s) 679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
AflII CTTAAG 1 cut(s) 3208
AflIII ACRYGT 3 cut(s) 910, 1211, 2809
AgsI TTSAA 9 cut(s) 64, 125, 248, 304, 322, 485, 1192, 2556, 2608
AjiI CACGTC 1 cut(s) 2215
AjnI CCWGG 6 cut(s) 629, 1037, 1063, 1196, 1648, 2252
AjuI GAANNNNNNNTTGG 4 cut(s) 1820, 1823, 1852, 1855
AleI CACNNNNGTG 1 cut(s) 2470
Alw21I GWGCWC 2 cut(s) 706, 2400
Alw26I GTCTC 2 cut(s) 1981, 2304
Alw44I GTGCAC 1 cut(s) 2396
AlwI GGATC 8 cut(s) 273, 1696, 2125, 2264, 2970, 3242, 3255, 3396
AlwNI CAGNNNCTG 4 cut(s) 46, 905, 1586, 2259
Ama87I CYCGRG 2 cut(s) 2833, 2943
AoxI GGCC 9 cut(s) 569, 762, 803, 1239, 1513, 2094, 2445, 2794, 3356
ApaLI GTGCAC 1 cut(s) 2396
ApoI RAATTY 6 cut(s) 59, 1868, 2001, 2011, 2356, 2894
Asp700I GAANNNNTTC 1 cut(s) 1835
AspLEI GCGC 3 cut(s) 736, 852, 1648
AspS9I GGNCC 8 cut(s) 710, 803, 1201, 1513, 2095, 3043, 3062, 3356
AsuC2I CCSGG 4 cut(s) 802, 878, 2944, 2945
AsuHPI GGTGA 7 cut(s) 145, 172, 413, 765, 1148, 1361, 1862
AvaI CYCGRG 2 cut(s) 2833, 2943
AvaII GGWCC 4 cut(s) 710, 1201, 3043, 3062
AxyI CCTNAGG 1 cut(s) 3155
BaeGI GKGCMC 3 cut(s) 1544, 2400, 3329
BaeI ACNNNNGTAYC 2 cut(s) 2931, 2964
BalI TGGCCA 2 cut(s) 764, 1241
BamHI GGATCC 1 cut(s) 3247
BanI GGYRCC 3 cut(s) 626, 2167, 3326
BanII GRGCYC 3 cut(s) 637, 841, 1628
BauI CACGAG 1 cut(s) 1702
Bbv12I GWGCWC 2 cut(s) 706, 2400
BcgI CGANNNNNNTGC 6 cut(s) 2100, 2134, 3225, 3259, 3366, 3400
BciT130I CCWGG 6 cut(s) 631, 1039, 1065, 1198, 1650, 2254
BciVI GTATCC 2 cut(s) 2839, 3228
BcnI CCSGG 4 cut(s) 802, 878, 2944, 2945
BcoDI GTCTC 2 cut(s) 1981, 2304
BfmI CTRYAG 3 cut(s) 819, 1226, 3117
BfoI RGCGCY 1 cut(s) 853
BfrI CTTAAG 1 cut(s) 3208
BfuAI ACCTGC 3 cut(s) 736, 2488, 3371
BfuI GTATCC 2 cut(s) 2839, 3228
BglI GCCNNNNNGGC 1 cut(s) 2375
BglII AGATCT 1 cut(s) 213
BlpI GCTNAGC 2 cut(s) 11, 1494
Bme18I GGWCC 4 cut(s) 710, 1201, 3043, 3062
BmeT110I CYCGRG 2 cut(s) 2833, 2943
BmgBI CACGTC 1 cut(s) 2215
BmgT120I GGNCC 8 cut(s) 710, 803, 1201, 1513, 2095, 3043, 3062, 3356
BmrI ACTGGG 2 cut(s) 1091, 1497
BmsI GCATC 9 cut(s) 1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
BmuI ACTGGG 2 cut(s) 1091, 1497
BoxI GACNNNNGTC 1 cut(s) 1657
BpmI CTGGAG 1 cut(s) 3393
Bpu1102I GCTNAGC 2 cut(s) 11, 1494
BpuEI CTTGAG 4 cut(s) 490, 1612, 3017, 3317
BpuMI CCSGG 4 cut(s) 802, 878, 2944, 2945
Bsa29I ATCGAT 2 cut(s) 1259, 1834
BsaBI GATNNNNATC 1 cut(s) 3252
BsaHI GRCGYC 1 cut(s) 1140
BsaI GGTCTC 1 cut(s) 2304
BsaJI CCNNGG 9 cut(s) 183, 566, 630, 1003, 1037, 1197, 2175, 2832, 2943
BsaWI WCCGGW 1 cut(s) 2164
BsaXI ACNNNNNCTCC 8 cut(s) 77, 107, 1319, 1349, 3276, 3306, 3319, 3349
Bsc4I CCNNNNNNNGG 9 cut(s) 679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
Bse118I RCCGGY 2 cut(s) 1927, 2295
Bse1I ACTGG 7 cut(s) 545, 965, 980, 1097, 1393, 1492, 3097
Bse21I CCTNAGG 1 cut(s) 3155
Bse3DI GCAATG 2 cut(s) 2334, 2697
Bse8I GATNNNNATC 1 cut(s) 3252
BseBI CCWGG 6 cut(s) 631, 1039, 1065, 1198, 1650, 2254
BseCI ATCGAT 2 cut(s) 1259, 1834
BseDI CCNNGG 9 cut(s) 183, 566, 630, 1003, 1037, 1197, 2175, 2832, 2943
BseJI GATNNNNATC 1 cut(s) 3252
BseLI CCNNNNNNNGG 9 cut(s) 679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
BseMI GCAATG 2 cut(s) 2334, 2697
BseMII CTCAG 4 cut(s) 2024, 2294, 2861, 3169
BseNI ACTGG 7 cut(s) 545, 965, 980, 1097, 1393, 1492, 3097
BseRI GAGGAG 1 cut(s) 852
BseSI GKGCMC 3 cut(s) 1544, 2400, 3329
BseYI CCCAGC 3 cut(s) 2098, 2653, 3353
BsgI GTGCAG 1 cut(s) 1832
Bsh1236I CGCG 2 cut(s) 3233, 3415
BshFI GGCC 9 cut(s) 571, 764, 805, 1241, 1515, 2096, 2447, 2796, 3358
BshNI GGYRCC 3 cut(s) 626, 2167, 3326
BshVI ATCGAT 2 cut(s) 1259, 1834
BsiHKAI GWGCWC 2 cut(s) 706, 2400
BsiHKCI CYCGRG 2 cut(s) 2833, 2943
BsiSI CCGG 6 cut(s) 801, 878, 1928, 2165, 2296, 2944
BslFI GGGAC 5 cut(s) 656, 1214, 1723, 1889, 3029
BslI CCNNNNNNNGG 9 cut(s) 679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
BsmAI GTCTC 2 cut(s) 1981, 2304
BsmFI GGGAC 5 cut(s) 656, 1214, 1723, 1889, 3029
BsmI GAATGC 2 cut(s) 317, 3032
BsnI GGCC 9 cut(s) 571, 764, 805, 1241, 1515, 2096, 2447, 2796, 3358
Bso31I GGTCTC 1 cut(s) 2304
BsoBI CYCGRG 2 cut(s) 2833, 2943
Bsp1286I GDGCHC 7 cut(s) 637, 706, 841, 1544, 1628, 2400, 3329
Bsp1720I GCTNAGC 2 cut(s) 11, 1494
Bsp19I CCATGG 2 cut(s) 1003, 2175
BspACI CCGC 7 cut(s) 514, 798, 1544, 1795, 3233, 3366, 3415
BspANI GGCC 9 cut(s) 571, 764, 805, 1241, 1515, 2096, 2447, 2796, 3358
BspCNI CTCAG 4 cut(s) 2025, 2295, 2860, 3168
BspDI ATCGAT 2 cut(s) 1259, 1834
BspFNI CGCG 2 cut(s) 3233, 3415
BspHI TCATGA 2 cut(s) 1050, 2986
BspMAI CTGCAG 3 cut(s) 823, 1230, 3121
BspMI ACCTGC 3 cut(s) 736, 2488, 3371
BspPI GGATC 8 cut(s) 273, 1696, 2125, 2264, 2970, 3242, 3255, 3396
BspT107I GGYRCC 3 cut(s) 626, 2167, 3326
BspTI CTTAAG 1 cut(s) 3208
BspTNI GGTCTC 1 cut(s) 2304
BsrDI GCAATG 2 cut(s) 2334, 2697
BsrFI RCCGGY 2 cut(s) 1927, 2295
BsrI ACTGG 7 cut(s) 545, 965, 980, 1097, 1393, 1492, 3097
BssAI RCCGGY 2 cut(s) 1927, 2295
BssECI CCNNGG 9 cut(s) 183, 566, 630, 1003, 1037, 1197, 2175, 2832, 2943
BssNAI GTATAC 1 cut(s) 1531
BssNI GRCGYC 1 cut(s) 1140
BssSI CACGAG 1 cut(s) 1702
BssT1I CCWWGG 4 cut(s) 183, 566, 1003, 2175
Bst1107I GTATAC 1 cut(s) 1531
Bst2BI CACGAG 1 cut(s) 1702
Bst2UI CCWGG 6 cut(s) 631, 1039, 1065, 1198, 1650, 2254
Bst6I CTCTTC 3 cut(s) 48, 147, 914
BstACI GRCGYC 1 cut(s) 1140
BstAFI CTTAAG 1 cut(s) 3208
BstC8I GCNNGC 9 cut(s) 233, 432, 1230, 1544, 1929, 2172, 2886, 2919, 2979
BstDEI CTNAG 9 cut(s) 11, 393, 1314, 1494, 2033, 2303, 2763, 2847, 3155
BstDSI CCRYGG 2 cut(s) 1003, 2175
BstFNI CGCG 2 cut(s) 3233, 3415
BstH2I RGCGCY 1 cut(s) 853
BstHHI GCGC 3 cut(s) 736, 852, 1648
BstMAI GTCTC 2 cut(s) 1981, 2304
BstNI CCWGG 6 cut(s) 631, 1039, 1065, 1198, 1650, 2254
BstNSI RCATGY 5 cut(s) 914, 1215, 2813, 3077, 3293
BstPAI GACNNNNGTC 1 cut(s) 1657
BstSFI CTRYAG 3 cut(s) 819, 1226, 3117
BstSLI GKGCMC 3 cut(s) 1544, 2400, 3329
BstUI CGCG 2 cut(s) 3233, 3415
BstX2I RGATCY 3 cut(s) 213, 2256, 3247
BstXI CCANNNNNNTGG 5 cut(s) 611, 1004, 2472, 2668, 2710
BstYI RGATCY 3 cut(s) 213, 2256, 3247
BstZ17I GTATAC 1 cut(s) 1531
Bsu15I ATCGAT 2 cut(s) 1259, 1834
Bsu36I CCTNAGG 1 cut(s) 3155
BsuI GTATCC 2 cut(s) 2839, 3228
BsuRI GGCC 9 cut(s) 571, 764, 805, 1241, 1515, 2096, 2447, 2796, 3358
BsuTUI ATCGAT 2 cut(s) 1259, 1834
BtgI CCRYGG 2 cut(s) 1003, 2175
BtrI CACGTC 1 cut(s) 2215
BtsI GCAGTG 2 cut(s) 1399, 3044
BtsIMutI CAGTG 8 cut(s) 1104, 1224, 1399, 1485, 1684, 1891, 2046, 3044
BveI ACCTGC 3 cut(s) 736, 2488, 3371
Cac8I GCNNGC 9 cut(s) 233, 432, 1230, 1544, 1929, 2172, 2886, 2919, 2979
CaiI CAGNNNCTG 4 cut(s) 46, 905, 1586, 2259
CciI TCATGA 2 cut(s) 1050, 2986
CfoI GCGC 3 cut(s) 736, 852, 1648
Cfr10I RCCGGY 2 cut(s) 1927, 2295
Cfr13I GGNCC 8 cut(s) 710, 803, 1201, 1513, 2095, 3043, 3062, 3356
Cfr9I CCCGGG 1 cut(s) 2943
ClaI ATCGAT 2 cut(s) 1259, 1834
Csp6I GTAC 8 cut(s) 118, 241, 1675, 1773, 1905, 2052, 2462, 3293
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviQI GTAC 8 cut(s) 118, 241, 1675, 1773, 1905, 2052, 2462, 3293
DdeI CTNAG 9 cut(s) 11, 393, 1314, 1494, 2033, 2303, 2763, 2847, 3155
DraIII CACNNNGTG 2 cut(s) 701, 1099
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 762, 1239
Eam1104I CTCTTC 3 cut(s) 48, 147, 914
EarI CTCTTC 3 cut(s) 48, 147, 914
EciI GGCGGA 1 cut(s) 787
Eco130I CCWWGG 4 cut(s) 183, 566, 1003, 2175
Eco147I AGGCCT 2 cut(s) 571, 2796
Eco24I GRGCYC 3 cut(s) 637, 841, 1628
Eco31I GGTCTC 1 cut(s) 2304
Eco47I GGWCC 4 cut(s) 710, 1201, 3043, 3062
Eco57I CTGAAG 4 cut(s) 23, 2547, 2568, 2885
Eco81I CCTNAGG 1 cut(s) 3155
Eco88I CYCGRG 2 cut(s) 2833, 2943
EcoO109I RGGNCCY 3 cut(s) 1201, 2095, 3043
EcoRII CCWGG 6 cut(s) 629, 1037, 1063, 1196, 1648, 2252
EcoT14I CCWWGG 4 cut(s) 183, 566, 1003, 2175
EcoT38I GRGCYC 3 cut(s) 637, 841, 1628
ErhI CCWWGG 4 cut(s) 183, 566, 1003, 2175
FaqI GGGAC 5 cut(s) 656, 1214, 1723, 1889, 3029
FauI CCCGC 1 cut(s) 1551
FblI GTMKAC 3 cut(s) 600, 1530, 1661
FriOI GRGCYC 3 cut(s) 637, 841, 1628
GlaI GCGC 3 cut(s) 735, 851, 1647
GsaI CCCAGC 3 cut(s) 2102, 2657, 3357
GsuI CTGGAG 1 cut(s) 3393
HaeII RGCGCY 1 cut(s) 853
HaeIII GGCC 9 cut(s) 571, 764, 805, 1241, 1515, 2096, 2447, 2796, 3358
HapII CCGG 6 cut(s) 801, 878, 1928, 2165, 2296, 2944
HhaI GCGC 3 cut(s) 736, 852, 1648
Hin1I GRCGYC 1 cut(s) 1140
Hin6I GCGC 3 cut(s) 734, 850, 1646
HinP1I GCGC 3 cut(s) 734, 850, 1646
HincII GTYRAC 3 cut(s) 357, 1636, 1891
HindII GTYRAC 3 cut(s) 357, 1636, 1891
HindIII AAGCTT 2 cut(s) 249, 3205
HpaI GTTAAC 1 cut(s) 1891
HpaII CCGG 6 cut(s) 801, 878, 1928, 2165, 2296, 2944
HphI GGTGA 7 cut(s) 145, 172, 413, 765, 1148, 1361, 1862
HpyAV CCTTC 8 cut(s) 1715, 1751, 1947, 1952, 2086, 2437, 2812, 2909
HpyCH4IV ACGT 3 cut(s) 1140, 2214, 2571
HpyF3I CTNAG 9 cut(s) 11, 393, 1314, 1494, 2033, 2303, 2763, 2847, 3155
HpySE526I ACGT 3 cut(s) 1140, 2214, 2571
Hsp92I GRCGYC 1 cut(s) 1140
HspAI GCGC 3 cut(s) 734, 850, 1646
KflI GGGWCCC 1 cut(s) 3043
KroI GCCGGC 1 cut(s) 1927
KroNI GCCGGC 1 cut(s) 1929
KspAI GTTAAC 1 cut(s) 1891
LmnI GCTCC 8 cut(s) 85, 640, 836, 1760, 2108, 2167, 2837, 3374
LweI GCATC 9 cut(s) 1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
MaeII ACGT 3 cut(s) 1140, 2214, 2571
MaeIII GTNAC 7 cut(s) 133, 646, 940, 1136, 1155, 1441, 1483
MboII GAAGA 9 cut(s) 65, 137, 164, 223, 338, 931, 2540, 2712, 2726
MflI RGATCY 3 cut(s) 213, 2256, 3247
MhlI GDGCHC 7 cut(s) 637, 706, 841, 1544, 1628, 2400, 3329
MlsI TGGCCA 2 cut(s) 764, 1241
MluNI TGGCCA 2 cut(s) 764, 1241
MlyI GAGTC 7 cut(s) 101, 352, 511, 1288, 2056, 2246, 2558
MmeI TCCRAC 6 cut(s) 63, 764, 1102, 2116, 2161, 3362
Mox20I TGGCCA 2 cut(s) 764, 1241
MroNI GCCGGC 1 cut(s) 1927
MroXI GAANNNNTTC 1 cut(s) 1835
MscI TGGCCA 2 cut(s) 764, 1241
MslI CAYNNNNRTG 6 cut(s) 609, 1347, 2290, 2470, 2495, 2708
Msp20I TGGCCA 2 cut(s) 764, 1241
MspA1I CMGCKG 4 cut(s) 352, 1073, 2657, 3182
MspCI CTTAAG 1 cut(s) 3208
MspI CCGG 6 cut(s) 801, 878, 1928, 2165, 2296, 2944
Mva1269I GAATGC 2 cut(s) 317, 3032
MvaI CCWGG 6 cut(s) 631, 1039, 1065, 1198, 1650, 2254
MvnI CGCG 2 cut(s) 3233, 3415
NaeI GCCGGC 1 cut(s) 1929
NciI CCSGG 4 cut(s) 802, 878, 2944, 2945
NcoI CCATGG 2 cut(s) 1003, 2175
NgoMIV GCCGGC 1 cut(s) 1927
NmuCI GTSAC 4 cut(s) 133, 1136, 1441, 1483
NspI RCATGY 5 cut(s) 914, 1215, 2813, 3077, 3293
OliI CACNNNNGTG 1 cut(s) 2470
PagI TCATGA 2 cut(s) 1050, 2986
PaqCI CACCTGC 1 cut(s) 736
PceI AGGCCT 2 cut(s) 571, 2796
PciI ACATGT 3 cut(s) 910, 1211, 2809
PctI GAATGC 2 cut(s) 317, 3032
PdiI GCCGGC 1 cut(s) 1929
PdmI GAANNNNTTC 1 cut(s) 1835
PflMI CCANNNNNTGG 1 cut(s) 2259
PfoI TCCNGGA 1 cut(s) 2252
PleI GAGTC 7 cut(s) 100, 352, 511, 1287, 2055, 2245, 2558
PpsI GAGTC 7 cut(s) 100, 352, 511, 1287, 2055, 2245, 2558
PpuMI RGGWCCY 2 cut(s) 1201, 3043
PscI ACATGT 3 cut(s) 910, 1211, 2809
PshAI GACNNNNGTC 1 cut(s) 1657
Psp5II RGGWCCY 2 cut(s) 1201, 3043
Psp6I CCWGG 6 cut(s) 629, 1037, 1063, 1196, 1648, 2252
PspFI CCCAGC 3 cut(s) 2098, 2653, 3353
PspGI CCWGG 6 cut(s) 629, 1037, 1063, 1196, 1648, 2252
PspPI GGNCC 8 cut(s) 710, 803, 1201, 1513, 2095, 3043, 3062, 3356
PspPPI RGGWCCY 2 cut(s) 1201, 3043
PsrI GAACNNNNNNTAC 2 cut(s) 850, 882
PstI CTGCAG 3 cut(s) 823, 1230, 3121
PstNI CAGNNNCTG 4 cut(s) 46, 905, 1586, 2259
PsuI RGATCY 3 cut(s) 213, 2256, 3247
PvuII CAGCTG 4 cut(s) 352, 1073, 2657, 3182
RsaI GTAC 8 cut(s) 119, 242, 1676, 1774, 1906, 2053, 2463, 3294
RsaNI GTAC 8 cut(s) 118, 241, 1675, 1773, 1905, 2052, 2462, 3293
RseI CAYNNNNRTG 6 cut(s) 609, 1347, 2290, 2470, 2495, 2708
Sau96I GGNCC 8 cut(s) 710, 803, 1201, 1513, 2095, 3043, 3062, 3356
SchI GAGTC 7 cut(s) 101, 352, 511, 1288, 2056, 2246, 2558
SduI GDGCHC 7 cut(s) 637, 706, 841, 1544, 1628, 2400, 3329
SfaNI GCATC 9 cut(s) 1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
SfcI CTRYAG 3 cut(s) 819, 1226, 3117
SinI GGWCC 4 cut(s) 710, 1201, 3043, 3062
SmaI CCCGGG 1 cut(s) 2945
SmiMI CAYNNNNRTG 6 cut(s) 609, 1347, 2290, 2470, 2495, 2708
SmlI CTYRAG 5 cut(s) 505, 1627, 3032, 3208, 3296
SmoI CTYRAG 5 cut(s) 505, 1627, 3032, 3208, 3296
SseBI AGGCCT 2 cut(s) 571, 2796
SsiI CCGC 7 cut(s) 514, 798, 1544, 1795, 3233, 3366, 3415
SspI AATATT 2 cut(s) 973, 3174
StuI AGGCCT 2 cut(s) 571, 2796
StyI CCWWGG 4 cut(s) 183, 566, 1003, 2175
TaiI ACGT 3 cut(s) 1143, 2217, 2574
TaqI TCGA 7 cut(s) 268, 1259, 1577, 1834, 2155, 2200, 2240
TatI WGTACW 2 cut(s) 1904, 3292
TauI GCSGC 2 cut(s) 3236, 3418
TscAI CASTG 8 cut(s) 1104, 1224, 1399, 1492, 1684, 1891, 2046, 3044
TseFI GTSAC 4 cut(s) 133, 1136, 1441, 1483
Tsp45I GTSAC 4 cut(s) 133, 1136, 1441, 1483
TspMI CCCGGG 1 cut(s) 2943
TspRI CASTG 8 cut(s) 1104, 1224, 1399, 1492, 1684, 1891, 2046, 3044
Van91I CCANNNNNTGG 1 cut(s) 2259
Vha464I CTTAAG 1 cut(s) 3208
VneI GTGCAC 1 cut(s) 2396
VpaK11BI GGWCC 4 cut(s) 710, 1201, 3043, 3062
XapI RAATTY 6 cut(s) 59, 1868, 2001, 2011, 2356, 2894
XbaI TCTAGA 1 cut(s) 1369
XceI RCATGY 5 cut(s) 914, 1215, 2813, 3077, 3293
XmaI CCCGGG 1 cut(s) 2943
XmiI GTMKAC 3 cut(s) 600, 1530, 1661
XmnI GAANNNNTTC 1 cut(s) 1835
ZraI GACGTC 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.