Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001857

Physical Location & Seq

Genomic Coordinates Forward (+)

13066327 .. 13075205

Gene Structure 8879 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1169300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3396 bp

ATGTCCTCGCTTAGCAGAGAGCTTGTTTTTCTCATACTTCAGTTTCTGGATGAAGAGAAATTCAAAGACAGTGTTCACAAGTTGGAGCAAGAGTCAGGGTTTTTCTTTAACATGAGGTACTTTGAGGACATGGTGACAACTGGGGAGTGGGAGGAGGTGGAAAAGTATTTGTCCGGTTTCACCAAGGTTGACGATAATCGATACTCTATGAAGATTTTCTTCGAGATTCGCAAGCAGAAGTACCTTGAAGCTTTGGACAAGAGGGATCGAGCAAAAGCTGTGGACATTTTAGTTAAGGACTTGAAAGTTTTTGCAGCATTTAATGAGGAACTTTTCAAGGAAATTACACAGCTGTTGACTTTGGAAAACTTTAGGGATAATGAACAGTTATCTAAATATGGTGATACTAAGTCTGCAAGGGGTATAATGCTTGCTGAATTGAAAAAGTTAATAGAGGCTAACCCATTGTTTCGTGACAAACTTCAATTTCCTACCCTGAAGAACTCGAGACTGCGTACTCTTATTAATCAGAGTTTAAACTGGCAGCATCAGCTTTGTAAGAACCCAAAGCCTAATCCAGACATAAAGACCCTGTTTGTAGACCACAGTTGTGGACAACCAAATGGTACCAGGGCTCCATCCCCCGTCACTAATCACTTAATGGGGGCAGTCCCTAAGACAGGGGGTTTCCCTCCATTGGGTGCTCATGGGCCCTTTCAGCCCACACCAGCTGCTCTTCCAACATCTCTTGCTGGATGGATGGCAAATCCATCACCTGTACCTCATCCCTCAGCTTCTGCTGGGCCTCTTGGGTTGGCTGCAGCTAACAATGCAGCCATCTTAAAGCGCCCTAGGACTCCTCCAACCAGTAACCCAACTATGGACTATCAAACAGCTGATTCTGAGCACGTTCTGAAGAGATCAAGACCTTTCGGGATAACAGATGAGGCCAATAATCTGCCGGTAAATATGCTGCCTGTTGTGTATCCTAATCAGAGCCATGGTCAGAGTTCCTACTCTTCTGATGACTTGCCCAGGTCTGTTGTTATGACTTTATGTCCAGGTTCAACTGTCAAGAGTATGGATTTCCACCCAGTGCTACAAATTTTACTTCTTGTTGGAACAAACATGGGCGATGTCATGGTATATGAGCTACCAAGCCTTGAAAAGATTGCTATTAAGAACTTCAAAGTTTGGGACCTTGGAGCATGTTCAGCGGCTCTGCAGGCATCTTTGGCCAGTGATTATACAGCATCAATTAACCGTGTGATGTGGAGCCCTGATGGGACATTTTTTGGTGTTGCATACTCTAAGCACATTGTACACATATATTCCTACCATGGTGGTGATGATTTACGAAATCAACTAGAGATCGAGGCTCATGTTGGCAGCGTTAATGATCTTGCTTTCTCATATCCAAACAAACAGCTGTGCGTTATCACTTGCGGAGAGGATAGGGTCATTAAGGTTTGGGATGCAGCCACTGGGACTAAGCAGTATACTTTTGAGGGCCATGAAGCACCTGTATATTCAGTGTGTCCACATCACAAAGAGAAAATCCAGTTTATTTTCTCGACAGCTACTGATGGGAAAATAAAGGCATGGTTGTATGATAATATGGGCTCAAGGGTTGACTATGATGCACCAGGCTATTCATCTACCACTATGGCATATAGTGCAGATGGAGCAAGGTTGTTCTCATGTGGGACAAATAAAGAAGGGGATTCATATTTGGTGGAGTGGAATGAAAGTGAAGGAGCTGTAAAGCGGACCTTTCATGGTCTTGCAAAGCATACTGTTGGGGTTGTGCAGTTTGATACCACAAAAAATCGGTTCTTGGCTGCTGGTGATGAGTTCACAGTCAAATTTTGGGACATGGACAATGTTAACCTTTTGATGAGTACTGATGCAGATGGTGGATTGCCGGCTTCTCCCGTAATCAGATTTAACAAGGAAGGAATATTGTTAGCTGCCTCAACGAATGACAATGGTATTAAAATTCTGGCAAATTCAGATGGAATTAGGTTGCTAAGAACTGTGGAAAGCCGCACATTTGATGCTTCAAGAGCTGCTTCTGCAGCTGCTGTGAAGGCACCCCCGACAGGAACATTTGGGCCTTCCAACATTCCTATTGGAACAAGCATTGGAGATCGAGCTGCTCCTGTGGCAGCCATGGCTGGACTGAACAATGACAATAGAAGTTTGGTTGACGTTAAACCTAGAATTGCGGATGAGTCGGTTGAGAAATCCAGGATTTGGAAACTGACTGAAATCAGTGAACAATCACAATGCCGCTCTCTGAGGCTCCCTGATAGTTTAACAGCAATGAGGGTTTCTAGATTAATATATACAAATTCAGGACTTGCGGTGTTGGCTTTATCATCTAATGCTGTACACAAGCTCTGGAAATGGCAAAGAAATGAACGAATACATAAGGCCACAGCCAGTAGTCCACCGCAAATGTGGCAACCTGCTAGTGGAATATTGATGACTAATGATATAAGTGATACAAACCCTGAGGATGCTGTTCCATGCTTTGCACTGTCAAAGAACGACTCTTATGTTATGTCAGCTTCAGGGGGGAAAATATCCCTTTTCAATATGATGACATTTAAGACAATGACAACATTCATGCCCCCACCACCAGCTGCAACATATCTGGCATTTCATCCTCAAGACAATAATATCATTGCCATAGGAATGGACGATTCTTCTATCCAAATTTACAATGTTCGGGTTGACGAGGTTAAAACCAAGCTAAAAGGTCATCAGAAAAGAATAACAGGCCTTGCCTTCTCTCATACTCTTAATGTGCTTGTGTCTTCGGGTGCTGATTCCCAGTTATGCGTTTGGAACACAGATGGATGGGAGAAGCAGGCTAGTAAATTTCTTCAGATGCCAAGTGGCCGAGCTGCTGCTCCTCTTGCTGATACCCGAGTTCAGTTTCACTCAGATCAGACACATTTGCTGGCTGTTCATGAAACACAGATAGCCATATACGAAGCACCAAAATTGGAATGCCTTAAGCAGTGGGTTCCTCGAGAAGCTAGTGGTCCAATCACACATGCTGTATATTCCTGTGATAGCCAGTTAATTTATGTTAGCTTTGAAGATGGAAGTGTGGGGGTTCTCACTGCTCTTACACTTAGATTGAGATGCCGAATACTACCAACTGCTTACTTACCTTCAAATCCAAGCTTAAGGGTGTATCCTCTCGTTGTTGCCGCACATCCCTCGGAACCCAATCAGTTTGCATTAGGACTTACAGATGGTGGAGTCCATGTACTTGAGCCATTAGAGTCAGAAGGAAAATGGGGCACTAACCCTCCAATCGAAAATGGTGCTGGGCCTAGCACGACTTCTGGGGTAGCTGGTTCAGAACAACCCCAAAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1132

Amino Acids

124.45

Weight (kDa)

6.51

Isoelectric Point (pI)

40.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	1.7e-12	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.8e-63	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1.1e-24	TOPLESS, zinc finger domain
Beta-prop_RIG_2nd	PF23775	366 - 511	7.1e-07	RIG second beta-propeller
Beta-prop_WDR3_1st	PF25173	392 - 572	3.1e-16	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	413 - 571	7.3e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	416 - 628	1e-19	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	417 - 572	1.6e-09	WDR3 second beta-propeller domain
WDR55	PF24796	418 - 535	1.2e-06	WDR55
Beta-prop_THOC3	PF25174	418 - 676	7e-24	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	431 - 538	2.8e-11	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	456 - 639	9.7e-08	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	476 - 539	1.6e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	789 - 954	5.5e-11	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	795 - 1035	4.4e-16	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	796 - 957	1.4e-13	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	837 - 959	1.3e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	838 - 954	5.3e-16	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	838 - 1043	2.5e-16	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	839 - 1052	2.1e-11	THOC3 beta-propeller domain
WDR55	PF24796	839 - 953	5.9e-07	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	840 - 953	8e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	841 - 1041	1.7e-18	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	844 - 961	4.9e-12	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	856 - 960	2.1e-09	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	861 - 964	2.7e-08	WDR90/POC16, second beta-propeller
WD40	PF00400	917 - 952	9.5e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15880 AT3G15880 AT3G15880 AT3G15880
fragaria_vesca	FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730 FvH4_1g02730
malus_domestica	MD02G1027700.v1.1 MD11G1043800.v1.1 MD15G1169300.v1.1
prunus_persica	Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.3G087700_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1 Prupe.7G247300_v2.0.a1
pyrus_communis	pycom12419g00020
rosa_chinensis	RchiOBHm_Chr2g0087841
rosa_laevigata	RLG00000015906 RLG00000019456
rosa_multiflora	Rmu_sc0008385.1_g000016
rosa_roxburghii	Rroxscaffold_2G00153180
rosa_rugosa	Rorug01G0479000 Rorug01G0479000 Rorug02G0324100 Rorug02G0324100
rosa_samantha	Rh2BG032700
rosa_wichuraiana	Rw2G002610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2479
Acc65I	GGTACC	1 cut(s)	626
AccB1I	GGYRCC	2 cut(s)	626, 2092
AccB7I	CCANNNNNTGG	1 cut(s)	2256
AccBSI	CCGCTC	1 cut(s)	2295
AccI	GTMKAC	2 cut(s)	600, 1499
AciI	CCGC	9 cut(s)	1217, 1446, 1768, 2047, 2228, 2293, 2366, 2456, 3225
AclWI	GGATC	1 cut(s)	273
AcoI	YGGCCR	2 cut(s)	1236, 2905
AcsI	RAATTY	8 cut(s)	59, 1104, 1865, 1998, 2008, 2353, 2721, 2885
AcuI	CTGAAG	5 cut(s)	23, 518, 935, 2559, 2876
AdeI	CACNNNGTG	1 cut(s)	1096
AfaI	GTAC	9 cut(s)	119, 242, 517, 628, 780, 1323, 1903, 2394, 3285
AfiI	CCNNNNNNNGG	7 cut(s)	680, 697, 698, 880, 2102, 2256, 2477
AflII	CTTAAG	2 cut(s)	3023, 3199
AhdI	GACNNNNNGTC	1 cut(s)	1056
AjnI	CCWGG	5 cut(s)	629, 1034, 1060, 1645, 2249
AleI	CACNNNNGTG	1 cut(s)	609
Alw21I	GWGCWC	2 cut(s)	706, 909
Alw26I	GTCTC	1 cut(s)	502
AlwI	GGATC	1 cut(s)	273
AlwNI	CAGNNNCTG	5 cut(s)	46, 797, 1484, 1583, 2084
Ama87I	CYCGRG	3 cut(s)	505, 2934, 3039
ApaI	GGGCCC	1 cut(s)	714
ApoI	RAATTY	8 cut(s)	59, 1104, 1865, 1998, 2008, 2353, 2721, 2885
AseI	ATTAAT	2 cut(s)	525, 2342
Asp700I	GAANNNNTTC	1 cut(s)	215
Asp718I	GGTACC	1 cut(s)	626
AspA2I	CCTAGG	1 cut(s)	851
AspLEI	GCGC	1 cut(s)	849
AspS9I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2114, 3053, 3347
AsuHPI	GGTGA	6 cut(s)	145, 172, 413, 765, 1358, 1859
AvaI	CYCGRG	3 cut(s)	505, 2934, 3039
AvaII	GGWCC	3 cut(s)	1198, 1770, 3053
AvrII	CCTAGG	1 cut(s)	851
AxyI	CCTNAGG	1 cut(s)	2517
BaeGI	GKGCMC	2 cut(s)	714, 3320
BaeI	ACNNNNGTAYC	2 cut(s)	2922, 2955
BalI	TGGCCA	1 cut(s)	1238
BanI	GGYRCC	2 cut(s)	626, 2092
BanII	GRGCYC	4 cut(s)	637, 714, 1280, 1625
BbsI	GAAGAC	1 cut(s)	2814
Bbv12I	GWGCWC	2 cut(s)	706, 909
BbvCI	CCTCAGC	1 cut(s)	790
BcgI	CGANNNNNNTGC	4 cut(s)	3216, 3250, 3323, 3357
BciT130I	CCWGG	5 cut(s)	631, 1036, 1062, 1647, 2251
BciVI	GTATCC	2 cut(s)	996, 3219
BcoDI	GTCTC	1 cut(s)	502
BfaI	CTAG	8 cut(s)	852, 1367, 2220, 2337, 2475, 2880, 3048, 3351
BfmI	CTRYAG	3 cut(s)	819, 1223, 2076
BfoI	RGCGCY	1 cut(s)	850
BfrI	CTTAAG	2 cut(s)	3023, 3199
BfuAI	ACCTGC	1 cut(s)	2479
BfuI	GTATCC	2 cut(s)	996, 3219
BlnI	CCTAGG	1 cut(s)	851
BlpI	GCTNAGC	1 cut(s)	11
BmcAI	AGTACT	1 cut(s)	1903
Bme1390I	CCNGG	5 cut(s)	631, 1036, 1062, 1647, 2251
Bme18I	GGWCC	3 cut(s)	1198, 1770, 3053
BmeRI	GACNNNNNGTC	1 cut(s)	1056
BmeT110I	CYCGRG	3 cut(s)	505, 2934, 3039
BmgT120I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2114, 3053, 3347
BmiI	GGNNCC	9 cut(s)	628, 636, 712, 1199, 1277, 2094, 2306, 3036, 3240
BmrFI	CCNGG	5 cut(s)	631, 1036, 1062, 1647, 2251
BmrI	ACTGGG	4 cut(s)	150, 1088, 1494, 2833
BmuI	ACTGGG	4 cut(s)	150, 1088, 1494, 2833
BpiI	GAAGAC	1 cut(s)	2814
Bpu10I	CCTNAGC	1 cut(s)	790
Bpu1102I	GCTNAGC	1 cut(s)	11
BpuEI	CTTGAG	3 cut(s)	1609, 2658, 3308
Bsa29I	ATCGAT	1 cut(s)	199
BsaJI	CCNNGG	9 cut(s)	183, 630, 851, 1000, 1034, 1201, 1339, 2172, 3234
BsaWI	WCCGGW	1 cut(s)	173
BsaXI	ACNNNNNCTCC	8 cut(s)	77, 107, 619, 649, 3267, 3297, 3310, 3340
Bsc4I	CCNNNNNNNGG	7 cut(s)	680, 697, 698, 880, 2102, 2256, 2477
Bse118I	RCCGGY	2 cut(s)	961, 1924
Bse21I	CCTNAGG	1 cut(s)	2517
Bse3DI	GCAATG	2 cut(s)	2331, 2688
BseBI	CCWGG	5 cut(s)	631, 1036, 1062, 1647, 2251
BseCI	ATCGAT	1 cut(s)	199
BseDI	CCNNGG	9 cut(s)	183, 630, 851, 1000, 1034, 1201, 1339, 2172, 3234
BseLI	CCNNNNNNNGG	7 cut(s)	680, 697, 698, 880, 2102, 2256, 2477
BseMI	GCAATG	2 cut(s)	2331, 2688
BseMII	CTCAG	5 cut(s)	804, 894, 2291, 2508, 2964
BseRI	GAGGAG	3 cut(s)	167, 849, 2910
BseSI	GKGCMC	2 cut(s)	714, 3320
BseYI	CCCAGC	2 cut(s)	800, 3344
BsgI	GTGCAG	2 cut(s)	1698, 1829
BshNI	GGYRCC	2 cut(s)	626, 2092
BshVI	ATCGAT	1 cut(s)	199
BsiHKAI	GWGCWC	2 cut(s)	706, 909
BsiHKCI	CYCGRG	3 cut(s)	505, 2934, 3039
BsiSI	CCGG	3 cut(s)	174, 962, 1925
BslFI	GGGAC	6 cut(s)	656, 1211, 1300, 1501, 1720, 1886
BslI	CCNNNNNNNGG	7 cut(s)	680, 697, 698, 880, 2102, 2256, 2477
BsmAI	GTCTC	1 cut(s)	502
BsmFI	GGGAC	6 cut(s)	656, 1211, 1300, 1501, 1720, 1886
BsmI	GAATGC	1 cut(s)	3023
BsoBI	CYCGRG	3 cut(s)	505, 2934, 3039
Bsp120I	GGGCCC	1 cut(s)	710
Bsp1286I	GDGCHC	7 cut(s)	637, 706, 714, 909, 1280, 1625, 3320
Bsp1407I	TGTACA	2 cut(s)	1321, 2392
Bsp143I	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
Bsp1720I	GCTNAGC	1 cut(s)	11
Bsp19I	CCATGG	3 cut(s)	1000, 1339, 2172
BspACI	CCGC	9 cut(s)	1217, 1446, 1768, 2047, 2228, 2293, 2366, 2456, 3225
BspCNI	CTCAG	5 cut(s)	803, 895, 2292, 2509, 2963
BspDI	ATCGAT	1 cut(s)	199
BspHI	TCATGA	1 cut(s)	2977
BspLI	GGNNCC	9 cut(s)	628, 636, 712, 1199, 1277, 2094, 2306, 3036, 3240
BspMAI	CTGCAG	3 cut(s)	823, 1227, 2080
BspMI	ACCTGC	1 cut(s)	2479
BspPI	GGATC	1 cut(s)	273
BspQI	GCTCTTC	1 cut(s)	741
BspT107I	GGYRCC	2 cut(s)	626, 2092
BspTI	CTTAAG	2 cut(s)	3023, 3199
BsrBI	CCGCTC	1 cut(s)	2295
BsrDI	GCAATG	2 cut(s)	2331, 2688
BsrFI	RCCGGY	2 cut(s)	961, 1924
BsrGI	TGTACA	2 cut(s)	1321, 2392
BssAI	RCCGGY	2 cut(s)	961, 1924
BssECI	CCNNGG	9 cut(s)	183, 630, 851, 1000, 1034, 1201, 1339, 2172, 3234
BssMI	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
BssNAI	GTATAC	1 cut(s)	1500
BssT1I	CCWWGG	6 cut(s)	183, 851, 1000, 1201, 1339, 2172
Bst1107I	GTATAC	1 cut(s)	1500
Bst2UI	CCWGG	5 cut(s)	631, 1036, 1062, 1647, 2251
Bst4CI	ACNGT	9 cut(s)	71, 387, 608, 1072, 1265, 1798, 1861, 2038, 2544
Bst6I	CTCTTC	4 cut(s)	48, 741, 911, 1024
BstAFI	CTTAAG	2 cut(s)	3023, 3199
BstAPI	GCANNNNNTGC	1 cut(s)	1676
BstAUI	TGTACA	2 cut(s)	1321, 2392
BstC8I	GCNNGC	6 cut(s)	233, 432, 1227, 1926, 2877, 2970
BstDSI	CCRYGG	3 cut(s)	1000, 1339, 2172
BstENI	CCTNNNNNAGG	1 cut(s)	678
BstH2I	RGCGCY	1 cut(s)	850
BstHHI	GCGC	1 cut(s)	849
BstKTI	GATC	6 cut(s)	268, 923, 1374, 1402, 2152, 2956
BstMAI	GTCTC	1 cut(s)	502
BstMBI	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
BstNI	CCWGG	5 cut(s)	631, 1036, 1062, 1647, 2251
BstNSI	RCATGY	2 cut(s)	1212, 3068
BstSCI	CCNGG	5 cut(s)	629, 1034, 1060, 1645, 2249
BstSFI	CTRYAG	3 cut(s)	819, 1223, 2076
BstSLI	GKGCMC	2 cut(s)	714, 3320
BstV2I	GAAGAC	1 cut(s)	2814
BstXI	CCANNNNNNTGG	2 cut(s)	611, 2701
BstZ17I	GTATAC	1 cut(s)	1500
Bsu15I	ATCGAT	1 cut(s)	199
Bsu36I	CCTNAGG	1 cut(s)	2517
BsuI	GTATCC	2 cut(s)	996, 3219
BsuTUI	ATCGAT	1 cut(s)	199
BtgI	CCRYGG	3 cut(s)	1000, 1339, 2172
BtgZI	GCGATG	1 cut(s)	1149
BtsI	GCAGTG	2 cut(s)	3035, 3132
BtsIMutI	CAGTG	9 cut(s)	76, 1101, 1246, 1482, 1539, 2281, 2540, 3035, 3132
BveI	ACCTGC	1 cut(s)	2479
Cac8I	GCNNGC	6 cut(s)	233, 432, 1227, 1926, 2877, 2970
CaiI	CAGNNNCTG	5 cut(s)	46, 797, 1484, 1583, 2084
CciI	TCATGA	1 cut(s)	2977
CfoI	GCGC	1 cut(s)	849
Cfr10I	RCCGGY	2 cut(s)	961, 1924
Cfr13I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2114, 3053, 3347
ClaI	ATCGAT	1 cut(s)	199
Csp6I	GTAC	9 cut(s)	118, 241, 516, 627, 779, 1322, 1902, 2393, 3284
CspCI	CAANNNNNGTGG	2 cut(s)	261, 296
CviQI	GTAC	9 cut(s)	118, 241, 516, 627, 779, 1322, 1902, 2393, 3284
DpnI	GATC	6 cut(s)	267, 922, 1373, 1401, 2151, 2955
DpnII	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
DraI	TTTAAA	1 cut(s)	537
DraIII	CACNNNGTG	1 cut(s)	1096
DriI	GACNNNNNGTC	1 cut(s)	1056
EaeI	YGGCCR	2 cut(s)	1236, 2905
Eam1104I	CTCTTC	4 cut(s)	48, 741, 911, 1024
Eam1105I	GACNNNNNGTC	1 cut(s)	1056
EarI	CTCTTC	4 cut(s)	48, 741, 911, 1024
Eco130I	CCWWGG	6 cut(s)	183, 851, 1000, 1201, 1339, 2172
Eco147I	AGGCCT	1 cut(s)	2787
Eco24I	GRGCYC	4 cut(s)	637, 714, 1280, 1625
Eco47I	GGWCC	3 cut(s)	1198, 1770, 3053
Eco57I	CTGAAG	5 cut(s)	23, 518, 935, 2559, 2876
Eco81I	CCTNAGG	1 cut(s)	2517
Eco88I	CYCGRG	3 cut(s)	505, 2934, 3039
EcoNI	CCTNNNNNAGG	1 cut(s)	678
EcoO109I	RGGNCCY	2 cut(s)	711, 1198
EcoRII	CCWGG	5 cut(s)	629, 1034, 1060, 1645, 2249
EcoT14I	CCWWGG	6 cut(s)	183, 851, 1000, 1201, 1339, 2172
EcoT38I	GRGCYC	4 cut(s)	637, 714, 1280, 1625
ErhI	CCWWGG	6 cut(s)	183, 851, 1000, 1201, 1339, 2172
FalI	AAGNNNNNCTT	6 cut(s)	203, 235, 1757, 1789, 2056, 2088
FaqI	GGGAC	6 cut(s)	656, 1211, 1300, 1501, 1720, 1886
FblI	GTMKAC	2 cut(s)	600, 1499
FriOI	GRGCYC	4 cut(s)	637, 714, 1280, 1625
FspBI	CTAG	8 cut(s)	852, 1367, 2220, 2337, 2475, 2880, 3048, 3351
GlaI	GCGC	1 cut(s)	848
GsaI	CCCAGC	2 cut(s)	804, 3348
HaeII	RGCGCY	1 cut(s)	850
HapII	CCGG	3 cut(s)	174, 962, 1925
HhaI	GCGC	1 cut(s)	849
Hin6I	GCGC	1 cut(s)	847
HinP1I	GCGC	1 cut(s)	847
HincII	GTYRAC	6 cut(s)	190, 357, 1633, 1888, 2209, 2740
HindII	GTYRAC	6 cut(s)	190, 357, 1633, 1888, 2209, 2740
HindIII	AAGCTT	2 cut(s)	249, 3196
HpaI	GTTAAC	1 cut(s)	1888
HpaII	CCGG	3 cut(s)	174, 962, 1925
HphI	GGTGA	6 cut(s)	145, 172, 413, 765, 1358, 1859
HpyAV	CCTTC	8 cut(s)	1712, 1748, 1949, 2083, 2127, 2803, 3195, 3299
HpyCH4III	ACNGT	9 cut(s)	71, 387, 608, 1072, 1265, 1798, 1861, 2038, 2544
HpyCH4IV	ACGT	2 cut(s)	909, 2211
HpySE526I	ACGT	2 cut(s)	909, 2211
HspAI	GCGC	1 cut(s)	847
KpnI	GGTACC	1 cut(s)	630
KroI	GCCGGC	1 cut(s)	1924
KroNI	GCCGGC	1 cut(s)	1926
KspAI	GTTAAC	1 cut(s)	1888
Kzo9I	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	9 cut(s)	85, 640, 1205, 1275, 1685, 1757, 2164, 2310, 2923
MaeI	CTAG	8 cut(s)	852, 1367, 2220, 2337, 2475, 2880, 3048, 3351
MaeII	ACGT	2 cut(s)	909, 2211
MaeIII	GTNAC	4 cut(s)	133, 473, 646, 869
MalI	GATC	6 cut(s)	267, 922, 1373, 1401, 2151, 2955
MbiI	CCGCTC	1 cut(s)	2295
MboI	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
MhlI	GDGCHC	7 cut(s)	637, 706, 714, 909, 1280, 1625, 3320
MlsI	TGGCCA	1 cut(s)	1238
MluNI	TGGCCA	1 cut(s)	1238
MlyI	GAGTC	6 cut(s)	101, 850, 2243, 2549, 3285, 3308
MmeI	TCCRAC	5 cut(s)	63, 764, 887, 1099, 2145
Mox20I	TGGCCA	1 cut(s)	1238
MroNI	GCCGGC	1 cut(s)	1924
MroXI	GAANNNNTTC	1 cut(s)	215
MscI	TGGCCA	1 cut(s)	1238
MslI	CAYNNNNRTG	3 cut(s)	609, 1344, 2699
Msp20I	TGGCCA	1 cut(s)	1238
MspA1I	CMGCKG	7 cut(s)	352, 731, 896, 1217, 1429, 2081, 2648
MspCI	CTTAAG	2 cut(s)	3023, 3199
MspI	CCGG	3 cut(s)	174, 962, 1925
MspR9I	CCNGG	5 cut(s)	631, 1036, 1062, 1647, 2251
MssI	GTTTAAAC	1 cut(s)	537
Mva1269I	GAATGC	1 cut(s)	3023
MvaI	CCWGG	5 cut(s)	631, 1036, 1062, 1647, 2251
NaeI	GCCGGC	1 cut(s)	1926
NcoI	CCATGG	3 cut(s)	1000, 1339, 2172
NdeII	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
NgoMIV	GCCGGC	1 cut(s)	1924
NlaIV	GGNNCC	9 cut(s)	628, 636, 712, 1199, 1277, 2094, 2306, 3036, 3240
NmeAIII	GCCGAG	1 cut(s)	2933
NmuCI	GTSAC	3 cut(s)	133, 473, 646
NspI	RCATGY	2 cut(s)	1212, 3068
OliI	CACNNNNGTG	1 cut(s)	609
PaeR7I	CTCGAG	2 cut(s)	505, 3039
PagI	TCATGA	1 cut(s)	2977
PceI	AGGCCT	1 cut(s)	2787
PciSI	GCTCTTC	1 cut(s)	741
PctI	GAATGC	1 cut(s)	3023
PdiI	GCCGGC	1 cut(s)	1926
PdmI	GAANNNNTTC	1 cut(s)	215
PfeI	GAWTC	5 cut(s)	226, 899, 1724, 2708, 2834
PflMI	CCANNNNNTGG	1 cut(s)	2256
PfoI	TCCNGGA	1 cut(s)	2249
PleI	GAGTC	6 cut(s)	100, 850, 2242, 2549, 3284, 3307
PmeI	GTTTAAAC	1 cut(s)	537
PpsI	GAGTC	6 cut(s)	100, 850, 2242, 2549, 3284, 3307
PpuMI	RGGWCCY	1 cut(s)	1198
PshBI	ATTAAT	2 cut(s)	525, 2342
Psp5II	RGGWCCY	1 cut(s)	1198
Psp6I	CCWGG	5 cut(s)	629, 1034, 1060, 1645, 2249
PspFI	CCCAGC	2 cut(s)	800, 3344
PspGI	CCWGG	5 cut(s)	629, 1034, 1060, 1645, 2249
PspN4I	GGNNCC	9 cut(s)	628, 636, 712, 1199, 1277, 2094, 2306, 3036, 3240
PspOMI	GGGCCC	1 cut(s)	710
PspPI	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2114, 3053, 3347
PspPPI	RGGWCCY	1 cut(s)	1198
PstI	CTGCAG	3 cut(s)	823, 1227, 2080
PstNI	CAGNNNCTG	5 cut(s)	46, 797, 1484, 1583, 2084
PvuII	CAGCTG	6 cut(s)	352, 731, 896, 1429, 2081, 2648
RsaI	GTAC	9 cut(s)	119, 242, 517, 628, 780, 1323, 1903, 2394, 3285
RsaNI	GTAC	9 cut(s)	118, 241, 516, 627, 779, 1322, 1902, 2393, 3284
RseI	CAYNNNNRTG	3 cut(s)	609, 1344, 2699
SapI	GCTCTTC	1 cut(s)	741
Sau3AI	GATC	6 cut(s)	265, 920, 1371, 1399, 2149, 2953
Sau96I	GGNCC	9 cut(s)	710, 711, 803, 1198, 1510, 1770, 2114, 3053, 3347
ScaI	AGTACT	1 cut(s)	1903
SchI	GAGTC	6 cut(s)	101, 850, 2243, 2549, 3285, 3308
ScrFI	CCNGG	5 cut(s)	631, 1036, 1062, 1647, 2251
SduI	GDGCHC	7 cut(s)	637, 706, 714, 909, 1280, 1625, 3320
SfcI	CTRYAG	3 cut(s)	819, 1223, 2076
Sfr274I	CTCGAG	2 cut(s)	505, 3039
SinI	GGWCC	3 cut(s)	1198, 1770, 3053
SlaI	CTCGAG	2 cut(s)	505, 3039
SmiMI	CAYNNNNRTG	3 cut(s)	609, 1344, 2699
SmlI	CTYRAG	7 cut(s)	505, 1624, 2673, 3023, 3039, 3199, 3287
SmoI	CTYRAG	7 cut(s)	505, 1624, 2673, 3023, 3039, 3199, 3287
SseBI	AGGCCT	1 cut(s)	2787
SsiI	CCGC	9 cut(s)	1217, 1446, 1768, 2047, 2228, 2293, 2366, 2456, 3225
SspI	AATATT	2 cut(s)	1962, 2484
SspMI	CTAG	8 cut(s)	852, 1367, 2220, 2337, 2475, 2880, 3048, 3351
StuI	AGGCCT	1 cut(s)	2787
StyD4I	CCNGG	5 cut(s)	629, 1034, 1060, 1645, 2249
StyI	CCWWGG	6 cut(s)	183, 851, 1000, 1201, 1339, 2172
TaaI	ACNGT	9 cut(s)	71, 387, 608, 1072, 1265, 1798, 1861, 2038, 2544
TaiI	ACGT	2 cut(s)	912, 2214
TaqI	TCGA	9 cut(s)	199, 222, 268, 506, 1374, 1574, 2152, 3040, 3333
TatI	WGTACW	4 cut(s)	1321, 1901, 2392, 3283
TauI	GCSGC	4 cut(s)	1220, 2049, 2295, 3227
TfiI	GAWTC	5 cut(s)	226, 899, 1724, 2708, 2834
TscAI	CASTG	9 cut(s)	76, 1101, 1246, 1489, 1539, 2281, 2547, 3035, 3139
TseFI	GTSAC	3 cut(s)	133, 473, 646
Tsp45I	GTSAC	3 cut(s)	133, 473, 646
TspRI	CASTG	9 cut(s)	76, 1101, 1246, 1489, 1539, 2281, 2547, 3035, 3139
Van91I	CCANNNNNTGG	1 cut(s)	2256
Vha464I	CTTAAG	2 cut(s)	3023, 3199
VpaK11BI	GGWCC	3 cut(s)	1198, 1770, 3053
VspI	ATTAAT	2 cut(s)	525, 2342
XagI	CCTNNNNNAGG	1 cut(s)	678
XapI	RAATTY	8 cut(s)	59, 1104, 1865, 1998, 2008, 2353, 2721, 2885
XbaI	TCTAGA	1 cut(s)	2336
XceI	RCATGY	2 cut(s)	1212, 3068
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2460
XhoI	CTCGAG	2 cut(s)	505, 3039
XmaJI	CCTAGG	1 cut(s)	851
XmiI	GTMKAC	2 cut(s)	600, 1499
XmnI	GAANNNNTTC	1 cut(s)	215
XspI	CTAG	8 cut(s)	852, 1367, 2220, 2337, 2475, 2880, 3048, 3351
ZrmI	AGTACT	1 cut(s)	1903

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.