MD02G1028200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007775
Physical Location & Seq
Forward (+)
2163935 .. 2166728
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1028200.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGCCTAGGCCGGGCCCGAGGCCGTATGAGTGTGTCCGGCGAGCTTGGCATAGCGATAGGCACCAATCCATTAGAGGTTCCATCATTCAGCAGATTTTCAGGGTCGTCAAAGAGGTTCACAGCTCCGTAACGAAGAATAACAAGGAATGGCAAGAGAAGCTGCCTTTGGTGGTTTTCAGAGCAGAGGAAATTATGTATTCCAAAGCCAATTCTGAGGCTGAGTACATGAATCTCGACACGCTATGGGACAGAGCAAATGATGCGGTTAACACAATTATCCGGCGAGATGAGGGCACTGAAACCGGGGAGCTTCTGCCGCCTTGTGTCGAAGCTGCCCTTAATCTTGGTTGCACTCCAGTAAGAGCTTCGAGAAGCCAACGCCACAGTAATCTGAGTTACCTCACTCCAAGAGCTCAAGACCCCGCTTCTACCCCCGCTAGGGTTTTGGATAAAACTACAGATGAACGGCGCCCTCAGTTTTCTCTGCATCACTCTAGCAATCAATTGAACTTTGCAAAAGCCTCAAATGTGAGTTCTGCGCATTTGGTCCCAGAATCTTACAGCCGCATCAACCAAAGTACTAACCTCACTAGCCCTCTGAGTTACCCTTTTGCACTTGAGAATGTTCTTGCTGGCCATAACCAGTTAGCAACAATGTCAACCAACAATCCCTTGAACTCGGGTTCAGTATATCCGTTATATTATGGAACTCACCATCAACCCCAAAAGCGCCAGGTCGTTCATCAGGCTCCAGAAAATACACATTCTAGGACTCTATATGTTGGTACTCCAATTGTTACATCAGTTATGGAGCCTACAAAGCAGAACCTTTTCTCCTCTCAAGGTGCTGAAAATGTGTCACACAGATTCACACAAGAAGTCATGGACACCCAGGAGAAGCCACGAGAAACAGAATGTGATTTATCATTGACGCTTGGTCCTGCTCTCCACCCATGTACTCAAAGAAGGTTGGCCAGTGAAATGGAAGATTTTGGTTCAAGAAATTCTCAAGAGGGGGGAAAGTTAAATGATGTTTCTTCACCGAGAAGTAAGGAATTCTGTTTCTTTCCCACAGAGACTGCTTATGATCGCGTTGAGTCCACTTCCAGTATGTGGAATTCAGAGAGCGGTCGTAGTTCTGAGGCAACTATAAGGAAGCGCAAGGCACCTTTTAGTACCAACGAGGAAGATAGACAATTTTGCTGGCAACCAGATGTCATTCCCAACAGGTTTACTGGTCGAACTACAGGGCCTGGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

421

Amino Acids

47.42

Weight (kDa)

7.69

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 540
AccB1I GGYRCC 3 cut(s) 60, 468, 1165
AccB7I CCANNNNNTGG 1 cut(s) 1113
AccBSI CCGCTC 1 cut(s) 1128
AccII CGCG 1 cut(s) 1092
AciI CCGC 6 cut(s) 263, 317, 423, 435, 565, 1128
AcoI YGGCCR 2 cut(s) 634, 972
AcsI RAATTY 3 cut(s) 1003, 1055, 1117
AcyI GRCGYC 1 cut(s) 469
AfaI GTAC 5 cut(s) 224, 580, 787, 958, 1177
AfiI CCNNNNNNNGG 4 cut(s) 11, 438, 439, 1113
AgsI TTSAA 3 cut(s) 508, 676, 999
AhdI GACNNNNNGTC 1 cut(s) 936
AjnI CCWGG 3 cut(s) 732, 891, 1252
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AloI GAACNNNNNNTCC 2 cut(s) 818, 850
AluBI AGCT 7 cut(s) 44, 123, 160, 310, 332, 365, 413
AluI AGCT 7 cut(s) 44, 123, 160, 310, 332, 365, 413
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 1 cut(s) 1070
AlwNI CAGNNNCTG 2 cut(s) 1079, 1253
Ama87I CYCGRG 2 cut(s) 16, 679
AoxI GGCC 6 cut(s) 8, 13, 20, 634, 972, 1250
ApaI GGGCCC 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 160, 332
ApoI RAATTY 3 cut(s) 1003, 1055, 1117
Asp700I GAANNNNTTC 1 cut(s) 830
AspA2I CCTAGG 1 cut(s) 5
AspLEI GCGC 4 cut(s) 471, 541, 732, 1161
AspS9I GGNCC 5 cut(s) 13, 14, 547, 938, 1250
AsuC2I CCSGG 2 cut(s) 12, 304
AsuHPI GGTGA 2 cut(s) 704, 1032
AvaI CYCGRG 2 cut(s) 16, 679
AvaII GGWCC 2 cut(s) 547, 938
AvrII CCTAGG 1 cut(s) 5
BaeGI GKGCMC 2 cut(s) 17, 296
BalI TGGCCA 2 cut(s) 636, 974
BanI GGYRCC 3 cut(s) 60, 468, 1165
BanII GRGCYC 2 cut(s) 17, 415
BauI CACGAG 1 cut(s) 903
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 2 cut(s) 147, 319
BccI CCATC 2 cut(s) 89, 723
BceAI ACGGC 2 cut(s) 7, 482
BciT130I CCWGG 3 cut(s) 734, 893, 1254
BcnI CCSGG 2 cut(s) 12, 304
BcoDI GTCTC 1 cut(s) 1070
BfaI CTAG 5 cut(s) 6, 438, 495, 591, 768
BfmI CTRYAG 2 cut(s) 456, 1245
BfoI RGCGCY 2 cut(s) 472, 733
BisI GCNGC 4 cut(s) 161, 317, 333, 565
BlnI CCTAGG 1 cut(s) 5
BlsI GCNGC 4 cut(s) 162, 318, 334, 566
BmcAI AGTACT 1 cut(s) 580
Bme1390I CCNGG 5 cut(s) 12, 304, 734, 893, 1254
Bme18I GGWCC 2 cut(s) 547, 938
BmeRI GACNNNNNGTC 1 cut(s) 936
BmeT110I CYCGRG 2 cut(s) 16, 679
BmgT120I GGNCC 5 cut(s) 13, 14, 547, 938, 1250
BmiI GGNNCC 8 cut(s) 15, 62, 79, 470, 549, 750, 813, 1167
BmrFI CCNGG 5 cut(s) 12, 304, 734, 893, 1254
BmsI GCATC 3 cut(s) 250, 496, 576
BpmI CTGGAG 2 cut(s) 339, 735
BpuEI CTTGAG 4 cut(s) 399, 638, 825, 993
BpuMI CCSGG 2 cut(s) 12, 304
BsaHI GRCGYC 1 cut(s) 469
BsaJI CCNNGG 4 cut(s) 5, 17, 303, 891
BsaXI ACNNNNNCTCC 2 cut(s) 818, 848
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 438, 439, 1113
Bse1I ACTGG 5 cut(s) 356, 643, 975, 1107, 1240
BseBI CCWGG 3 cut(s) 734, 893, 1254
BseDI CCNNGG 4 cut(s) 5, 17, 303, 891
BseLI CCNNNNNNNGG 4 cut(s) 11, 438, 439, 1113
BseMII CTCAG 6 cut(s) 204, 210, 383, 488, 590, 1131
BseNI ACTGG 5 cut(s) 356, 643, 975, 1107, 1240
BseRI GAGGAG 1 cut(s) 826
BseSI GKGCMC 2 cut(s) 17, 296
BseXI GCAGC 2 cut(s) 147, 319
Bsh1236I CGCG 1 cut(s) 1092
Bsh1285I CGRYCG 1 cut(s) 1132
BshFI GGCC 6 cut(s) 10, 15, 22, 636, 974, 1252
BshNI GGYRCC 3 cut(s) 60, 468, 1165
BsiEI CGRYCG 1 cut(s) 1132
BsiHKAI GWGCWC 1 cut(s) 415
BsiHKCI CYCGRG 2 cut(s) 16, 679
BsiSI CCGG 4 cut(s) 11, 37, 280, 303
BslFI GGGAC 2 cut(s) 260, 533
BslI CCNNNNNNNGG 4 cut(s) 11, 438, 439, 1113
BsmAI GTCTC 1 cut(s) 1070
BsmFI GGGAC 2 cut(s) 260, 533
BsnI GGCC 6 cut(s) 10, 15, 22, 636, 974, 1252
BsoBI CYCGRG 2 cut(s) 16, 679
Bsp120I GGGCCC 1 cut(s) 13
Bsp1286I GDGCHC 3 cut(s) 17, 296, 415
Bsp143I GATC 1 cut(s) 1087
BspACI CCGC 6 cut(s) 263, 317, 423, 435, 565, 1128
BspANI GGCC 6 cut(s) 10, 15, 22, 636, 974, 1252
BspCNI CTCAG 6 cut(s) 205, 211, 384, 487, 591, 1132
BspFNI CGCG 1 cut(s) 1092
BspLI GGNNCC 8 cut(s) 15, 62, 79, 470, 549, 750, 813, 1167
BspT107I GGYRCC 3 cut(s) 60, 468, 1165
BsrBI CCGCTC 1 cut(s) 1128
BsrI ACTGG 5 cut(s) 356, 643, 975, 1107, 1240
BssECI CCNNGG 4 cut(s) 5, 17, 303, 891
BssMI GATC 1 cut(s) 1087
BssNI GRCGYC 1 cut(s) 469
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 1 cut(s) 5
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 3 cut(s) 734, 893, 1254
Bst4CI ACNGT 1 cut(s) 386
BstACI GRCGYC 1 cut(s) 469
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 3 cut(s) 42, 634, 1205
BstDEI CTNAG 6 cut(s) 213, 219, 392, 474, 599, 1140
BstFNI CGCG 1 cut(s) 1092
BstH2I RGCGCY 2 cut(s) 472, 733
BstHHI GCGC 4 cut(s) 471, 541, 732, 1161
BstKTI GATC 1 cut(s) 1090
BstMAI GTCTC 1 cut(s) 1070
BstMBI GATC 1 cut(s) 1087
BstMCI CGRYCG 1 cut(s) 1132
BstMWI GCNNNNNNNGC 5 cut(s) 46, 157, 260, 316, 820
BstNI CCWGG 3 cut(s) 734, 893, 1254
BstSCI CCNGG 5 cut(s) 10, 302, 732, 891, 1252
BstSFI CTRYAG 2 cut(s) 456, 1245
BstSLI GKGCMC 2 cut(s) 17, 296
BstUI CGCG 1 cut(s) 1092
BstV1I GCAGC 2 cut(s) 147, 319
BstXI CCANNNNNNTGG 1 cut(s) 982
BsuRI GGCC 6 cut(s) 10, 15, 22, 636, 974, 1252
BtsIMutI CAGTG 2 cut(s) 294, 982
Cac8I GCNNGC 3 cut(s) 42, 634, 1205
CaiI CAGNNNCTG 2 cut(s) 1079, 1253
CfoI GCGC 4 cut(s) 471, 541, 732, 1161
Cfr13I GGNCC 5 cut(s) 13, 14, 547, 938, 1250
CseI GACGC 1 cut(s) 940
Csp6I GTAC 5 cut(s) 223, 579, 786, 957, 1176
CviAII CATG 3 cut(s) 226, 883, 954
CviQI GTAC 5 cut(s) 223, 579, 786, 957, 1176
DdeI CTNAG 6 cut(s) 213, 219, 392, 474, 599, 1140
DinI GGCGCC 1 cut(s) 470
DpnI GATC 1 cut(s) 1089
DpnII GATC 1 cut(s) 1087
DriI GACNNNNNGTC 1 cut(s) 936
EaeI YGGCCR 2 cut(s) 634, 972
Eam1105I GACNNNNNGTC 1 cut(s) 936
Ecl136II GAGCTC 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 5
Eco24I GRGCYC 2 cut(s) 17, 415
Eco47I GGWCC 2 cut(s) 547, 938
Eco53kI GAGCTC 1 cut(s) 413
Eco88I CYCGRG 2 cut(s) 16, 679
EcoICRI GAGCTC 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 1250
EcoRI GAATTC 2 cut(s) 1055, 1117
EcoRII CCWGG 3 cut(s) 732, 891, 1252
EcoT14I CCWWGG 1 cut(s) 5
EcoT38I GRGCYC 2 cut(s) 17, 415
EgeI GGCGCC 1 cut(s) 470
EheI GGCGCC 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 5
FaeI CATG 3 cut(s) 229, 886, 957
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 2 cut(s) 260, 533
FatI CATG 3 cut(s) 225, 882, 953
FauI CCCGC 2 cut(s) 430, 442
Fnu4HI GCNGC 4 cut(s) 161, 317, 333, 565
FriOI GRGCYC 2 cut(s) 17, 415
Fsp4HI GCNGC 4 cut(s) 161, 317, 333, 565
FspBI CTAG 5 cut(s) 6, 438, 495, 591, 768
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 4 cut(s) 470, 540, 731, 1160
GluI GCNGC 4 cut(s) 161, 317, 333, 565
GsuI CTGGAG 2 cut(s) 339, 735
HaeII RGCGCY 2 cut(s) 472, 733
HaeIII GGCC 6 cut(s) 10, 15, 22, 636, 974, 1252
HapII CCGG 4 cut(s) 11, 37, 280, 303
HgaI GACGC 1 cut(s) 940
HhaI GCGC 4 cut(s) 471, 541, 732, 1161
Hin1I GRCGYC 1 cut(s) 469
Hin1II CATG 3 cut(s) 229, 886, 957
Hin6I GCGC 4 cut(s) 469, 539, 730, 1159
HinP1I GCGC 4 cut(s) 469, 539, 730, 1159
HincII GTYRAC 2 cut(s) 268, 660
HindII GTYRAC 2 cut(s) 268, 660
HinfI GANTC 5 cut(s) 229, 554, 772, 867, 1097
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 4 cut(s) 11, 37, 280, 303
HphI GGTGA 2 cut(s) 704, 1032
Hpy166II GTNNAC 5 cut(s) 118, 268, 660, 1101, 1233
Hpy188I TCNGA 6 cut(s) 179, 214, 393, 600, 1123, 1141
Hpy188III TCNNGA 6 cut(s) 233, 369, 416, 752, 999, 1010
Hpy8I GTNNAC 5 cut(s) 118, 268, 660, 1101, 1233
HpyAV CCTTC 1 cut(s) 960
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4V TGCA 4 cut(s) 351, 487, 515, 614
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 157, 260, 316, 820
HpyF3I CTNAG 6 cut(s) 213, 219, 392, 474, 599, 1140
Hsp92I GRCGYC 1 cut(s) 469
Hsp92II CATG 3 cut(s) 229, 886, 957
HspAI GCGC 4 cut(s) 469, 539, 730, 1159
KasI GGCGCC 1 cut(s) 468
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 1 cut(s) 1087
LmnI GCTCC 4 cut(s) 128, 307, 754, 811
Lsp1109I GCAGC 2 cut(s) 147, 319
LweI GCATC 3 cut(s) 250, 496, 576
MaeI CTAG 5 cut(s) 6, 438, 495, 591, 768
MaeIII GTNAC 5 cut(s) 127, 395, 602, 796, 858
MalI GATC 1 cut(s) 1089
MbiI CCGCTC 1 cut(s) 1128
MboI GATC 1 cut(s) 1087
MboII GAAGA 4 cut(s) 145, 998, 1029, 1199
MfeI CAATTG 2 cut(s) 503, 792
MhlI GDGCHC 3 cut(s) 17, 296, 415
MlsI TGGCCA 2 cut(s) 636, 974
MluCI AATT 9 cut(s) 189, 208, 273, 503, 792, 1003, 1055, 1117, 1196
MluNI TGGCCA 2 cut(s) 636, 974
Mly113I GGCGCC 1 cut(s) 469
MlyI GAGTC 2 cut(s) 766, 1106
Mox20I TGGCCA 2 cut(s) 636, 974
MroXI GAANNNNTTC 1 cut(s) 830
MscI TGGCCA 2 cut(s) 636, 974
MseI TTAA 3 cut(s) 267, 339, 1025
Msp20I TGGCCA 2 cut(s) 636, 974
MspI CCGG 4 cut(s) 11, 37, 280, 303
MspR9I CCNGG 5 cut(s) 12, 304, 734, 893, 1254
MunI CAATTG 2 cut(s) 503, 792
MvaI CCWGG 3 cut(s) 734, 893, 1254
MvnI CGCG 1 cut(s) 1092
MwoI GCNNNNNNNGC 5 cut(s) 46, 157, 260, 316, 820
NarI GGCGCC 1 cut(s) 469
NciI CCSGG 2 cut(s) 12, 304
NdeII GATC 1 cut(s) 1087
NlaIII CATG 3 cut(s) 229, 886, 957
NlaIV GGNNCC 8 cut(s) 15, 62, 79, 470, 549, 750, 813, 1167
NmuCI GTSAC 1 cut(s) 858
NsbI TGCGCA 1 cut(s) 540
PdmI GAANNNNTTC 1 cut(s) 830
PfeI GAWTC 3 cut(s) 229, 554, 867
PflMI CCANNNNNTGG 1 cut(s) 1113
PkrI GCNGC 4 cut(s) 162, 318, 334, 566
PleI GAGTC 2 cut(s) 766, 1105
PluTI GGCGCC 1 cut(s) 472
PpsI GAGTC 2 cut(s) 766, 1105
Psp124BI GAGCTC 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 732, 891, 1252
PspGI CCWGG 3 cut(s) 732, 891, 1252
PspN4I GGNNCC 8 cut(s) 15, 62, 79, 470, 549, 750, 813, 1167
PspOMI GGGCCC 1 cut(s) 13
PspPI GGNCC 5 cut(s) 13, 14, 547, 938, 1250
PstNI CAGNNNCTG 2 cut(s) 1079, 1253
RsaI GTAC 5 cut(s) 224, 580, 787, 958, 1177
RsaNI GTAC 5 cut(s) 223, 579, 786, 957, 1176
SacI GAGCTC 1 cut(s) 415
SaqAI TTAA 3 cut(s) 267, 339, 1025
SatI GCNGC 4 cut(s) 161, 317, 333, 565
Sau3AI GATC 1 cut(s) 1087
Sau96I GGNCC 5 cut(s) 13, 14, 547, 938, 1250
ScaI AGTACT 1 cut(s) 580
SchI GAGTC 2 cut(s) 766, 1106
ScrFI CCNGG 5 cut(s) 12, 304, 734, 893, 1254
SduI GDGCHC 3 cut(s) 17, 296, 415
SfaNI GCATC 3 cut(s) 250, 496, 576
SfcI CTRYAG 2 cut(s) 456, 1245
SfoI GGCGCC 1 cut(s) 470
SinI GGWCC 2 cut(s) 547, 938
SmlI CTYRAG 4 cut(s) 414, 617, 840, 1008
SmoI CTYRAG 4 cut(s) 414, 617, 840, 1008
Sse9I AATT 9 cut(s) 189, 208, 273, 503, 792, 1003, 1055, 1117, 1196
SsiI CCGC 6 cut(s) 263, 317, 423, 435, 565, 1128
SspDI GGCGCC 1 cut(s) 468
SspMI CTAG 5 cut(s) 6, 438, 495, 591, 768
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 5 cut(s) 10, 302, 732, 891, 1252
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 1 cut(s) 386
TaqI TCGA 4 cut(s) 234, 327, 368, 1240
TasI AATT 9 cut(s) 189, 208, 273, 503, 792, 1003, 1055, 1117, 1196
TatI WGTACW 3 cut(s) 222, 578, 956
TauI GCSGC 2 cut(s) 319, 567
TfiI GAWTC 3 cut(s) 229, 554, 867
Tru1I TTAA 3 cut(s) 267, 339, 1025
Tru9I TTAA 3 cut(s) 267, 339, 1025
TscAI CASTG 2 cut(s) 301, 982
TseFI GTSAC 1 cut(s) 858
TseI GCWGC 2 cut(s) 160, 332
Tsp45I GTSAC 1 cut(s) 858
TspDTI ATGAA 3 cut(s) 242, 477, 731
TspGWI ACGGA 2 cut(s) 115, 684
TspRI CASTG 2 cut(s) 301, 982
Van91I CCANNNNNTGG 1 cut(s) 1113
VpaK11BI GGWCC 2 cut(s) 547, 938
XapI RAATTY 3 cut(s) 1003, 1055, 1117
XmaJI CCTAGG 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 830
XspI CTAG 5 cut(s) 6, 438, 495, 591, 768
ZrmI AGTACT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.