pycom02g02320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007775
Physical Location & Seq
Forward (+)
1427130 .. 1428763
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02320.3

Sequence Viewer

Length: 1266 bp
ATGCCTAGGCCGGGCCCGAGGCCGTATGAGTGTGTCCGGCGAGCTTGGCATAGCGATAGGCACCAACCCATGAGAGGTTCCATCATTCAGCAGATTTTCAGGGTCGTCAATGAGGTTCACAGCTCCGTAACGAAGAAGAACAAGGAATGGCAAGAGAAGCTGCCTTTGGTGGTTTTAAGAGCAGAGGAAATTATGTATTCCAAAGCCAATTCTGAGGCTGAGTACATGAATCTCGACACGCTATGGGACAGAGCAAATGACGCGGTTAACACGATTATCCGGCGAGATGAGGGCACTGAAACTGGGGAGCTTCTGCCGCCGTGTGTTGAAGCTGCCCTTAATCTGGGTTGCACTCCAGTAAGAGCTTCGAGAAGCCAACGCCACAGTAATCCGAGGAGTTACCTCACTCCAAGAGCTCAAGACCCCGCATCTACCCCTGCTAGGGTTTTGGATAAAACTACAGATGAACAGCGCCCTCAGTTTTCTCCGCATCACTCTAGCAATCAATTGAACTTTGCAAAAGCCTTGAATGTGAGTTCTGTGCATTTGGTCCCAGAATCTTACGGCCGCATCAACCAAAGTACTAACCTCACTAGCCCTCTGAATTACCCTTTTGCACTTGAGAATTTTCCTGCTGGCCATAACCAGTTAGCAACACTGTCAACCAACAATCCCTTGAACTTGGGTTCAGTATATCCATTATATTATGGAACTCACCATCAACCCCAAAAGCTCCAGGTCGGTCATCAGGCTTCAGAAAATACACATTCTATGACTATACATGTTGGTACTCCAATTGTTACATCAGTTGTGGAGCCTATAAAGCAGAACCTTTTCTCCTCTCAAGGTGCTGAAAATGTGTCACACGGATATACACAAGAAGTCATGGACACCCCGGAGAAGCCACGAGAAACGGAATGTGATTTATCGTTGACGCTGGGTCCTGCTCTCCACCCATCTACTCAAAGAAGCTTGGCCAGTGAAATGGAAGATTTTGGTTCAATAAATTCTCAAGAGTGGGGAAAGTTAAATGATTTTTCTTCACCGAGAAGTAAGGAAATCTGTTTCTTTCCCACAAAGACTGCTTATGATCGCTTTGAGTCCACTTCTAGTATGTGGAATTCAGAGAGCGGTCGGAGTTCTGAGGCAACTATAAGGAAGCGCAAGGCACCTTTTAGTACCAATGAGGAAGATAGACAATTTTGCTGGCAACCAGATGTCATTCCCAACAGGTTTACTGGTCGAACTACAGGGCCTGGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

47.38

Weight (kDa)

6.74

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 60, 1168
AccBSI CCGCTC 1 cut(s) 1131
AccII CGCG 1 cut(s) 263
AciI CCGC 6 cut(s) 263, 317, 426, 488, 568, 1131
AcoI YGGCCR 3 cut(s) 565, 637, 975
AcsI RAATTY 3 cut(s) 625, 1006, 1120
AcuI CTGAAG 1 cut(s) 738
AfaI GTAC 4 cut(s) 224, 583, 790, 1180
AfiI CCNNNNNNNGG 5 cut(s) 11, 74, 343, 441, 442
AflIII ACRYGT 1 cut(s) 781
AgsI TTSAA 5 cut(s) 329, 511, 529, 679, 1002
AhdI GACNNNNNGTC 1 cut(s) 939
AjnI CCWGG 2 cut(s) 735, 1255
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AloI GAACNNNNNNTCC 2 cut(s) 821, 853
AluBI AGCT 9 cut(s) 44, 123, 160, 310, 332, 365, 416, 733, 972
AluI AGCT 9 cut(s) 44, 123, 160, 310, 332, 365, 416, 733, 972
Alw21I GWGCWC 1 cut(s) 418
AlwNI CAGNNNCTG 1 cut(s) 1256
Ama87I CYCGRG 1 cut(s) 16
AoxI GGCC 7 cut(s) 8, 13, 20, 565, 637, 975, 1253
ApaI GGGCCC 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 160, 332
ApoI RAATTY 3 cut(s) 625, 1006, 1120
Asp700I GAANNNNTTC 1 cut(s) 833
AspA2I CCTAGG 1 cut(s) 5
AspLEI GCGC 2 cut(s) 474, 1164
AspS9I GGNCC 5 cut(s) 13, 14, 550, 941, 1253
AsuC2I CCSGG 2 cut(s) 12, 896
AsuHPI GGTGA 2 cut(s) 707, 1035
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 2 cut(s) 550, 941
AvrII CCTAGG 1 cut(s) 5
BaeGI GKGCMC 2 cut(s) 17, 296
BalI TGGCCA 2 cut(s) 639, 977
BanI GGYRCC 2 cut(s) 60, 1168
BanII GRGCYC 2 cut(s) 17, 418
BauI CACGAG 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 147, 319
BccI CCATC 3 cut(s) 89, 726, 964
BceAI ACGGC 3 cut(s) 7, 304, 580
BciT130I CCWGG 2 cut(s) 737, 1257
BcnI CCSGG 2 cut(s) 12, 896
BfaI CTAG 5 cut(s) 6, 441, 498, 594, 1110
BfmI CTRYAG 2 cut(s) 459, 1248
BfoI RGCGCY 1 cut(s) 475
BisI GCNGC 4 cut(s) 161, 317, 333, 568
BlnI CCTAGG 1 cut(s) 5
BlsI GCNGC 4 cut(s) 162, 318, 334, 569
BmcAI AGTACT 1 cut(s) 583
Bme1390I CCNGG 4 cut(s) 12, 737, 896, 1257
Bme18I GGWCC 2 cut(s) 550, 941
BmeRI GACNNNNNGTC 1 cut(s) 939
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 5 cut(s) 13, 14, 550, 941, 1253
BmiI GGNNCC 7 cut(s) 15, 62, 79, 552, 816, 942, 1170
BmrFI CCNGG 4 cut(s) 12, 737, 896, 1257
BmrI ACTGGG 1 cut(s) 312
BmsI GCATC 3 cut(s) 437, 499, 579
BmuI ACTGGG 1 cut(s) 312
BpmI CTGGAG 2 cut(s) 339, 719
BpuEI CTTGAG 4 cut(s) 402, 641, 828, 996
BpuMI CCSGG 2 cut(s) 12, 896
BsaJI CCNNGG 4 cut(s) 5, 17, 392, 894
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 74, 343, 441, 442
Bse1I ACTGG 5 cut(s) 307, 356, 646, 978, 1243
BseBI CCWGG 2 cut(s) 737, 1257
BseDI CCNNGG 4 cut(s) 5, 17, 392, 894
BseLI CCNNNNNNNGG 5 cut(s) 11, 74, 343, 441, 442
BseMII CTCAG 4 cut(s) 204, 210, 491, 1134
BseNI ACTGG 5 cut(s) 307, 356, 646, 978, 1243
BseRI GAGGAG 2 cut(s) 409, 829
BseSI GKGCMC 2 cut(s) 17, 296
BseX3I CGGCCG 1 cut(s) 565
BseXI GCAGC 2 cut(s) 147, 319
BseYI CCCAGC 1 cut(s) 937
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 2 cut(s) 568, 1135
BshFI GGCC 7 cut(s) 10, 15, 22, 567, 639, 977, 1255
BshNI GGYRCC 2 cut(s) 60, 1168
BsiEI CGRYCG 2 cut(s) 568, 1135
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 4 cut(s) 11, 37, 280, 896
BslFI GGGAC 2 cut(s) 260, 536
BslI CCNNNNNNNGG 5 cut(s) 11, 74, 343, 441, 442
BsmFI GGGAC 2 cut(s) 260, 536
BsnI GGCC 7 cut(s) 10, 15, 22, 567, 639, 977, 1255
BsoBI CYCGRG 1 cut(s) 16
Bsp120I GGGCCC 1 cut(s) 13
Bsp1286I GDGCHC 3 cut(s) 17, 296, 418
Bsp143I GATC 1 cut(s) 1090
BspACI CCGC 6 cut(s) 263, 317, 426, 488, 568, 1131
BspANI GGCC 7 cut(s) 10, 15, 22, 567, 639, 977, 1255
BspCNI CTCAG 4 cut(s) 205, 211, 490, 1135
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 7 cut(s) 15, 62, 79, 552, 816, 942, 1170
BspT107I GGYRCC 2 cut(s) 60, 1168
BsrBI CCGCTC 1 cut(s) 1131
BsrI ACTGG 5 cut(s) 307, 356, 646, 978, 1243
BssECI CCNNGG 4 cut(s) 5, 17, 392, 894
BssMI GATC 1 cut(s) 1090
BssSI CACGAG 1 cut(s) 906
BssT1I CCWWGG 1 cut(s) 5
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 2 cut(s) 737, 1257
Bst4CI ACNGT 2 cut(s) 386, 660
BstC8I GCNNGC 3 cut(s) 42, 637, 1208
BstDEI CTNAG 4 cut(s) 213, 219, 477, 1143
BstFNI CGCG 1 cut(s) 263
BstH2I RGCGCY 1 cut(s) 475
BstHHI GCGC 2 cut(s) 474, 1164
BstKTI GATC 1 cut(s) 1093
BstMBI GATC 1 cut(s) 1090
BstMCI CGRYCG 2 cut(s) 568, 1135
BstMWI GCNNNNNNNGC 5 cut(s) 46, 157, 260, 316, 823
BstNI CCWGG 2 cut(s) 737, 1257
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 4 cut(s) 10, 735, 894, 1255
BstSFI CTRYAG 2 cut(s) 459, 1248
BstSLI GKGCMC 2 cut(s) 17, 296
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 147, 319
BstXI CCANNNNNNTGG 1 cut(s) 985
BstZI CGGCCG 1 cut(s) 565
BsuRI GGCC 7 cut(s) 10, 15, 22, 567, 639, 977, 1255
BtsIMutI CAGTG 3 cut(s) 294, 656, 985
Cac8I GCNNGC 3 cut(s) 42, 637, 1208
CaiI CAGNNNCTG 1 cut(s) 1256
CfoI GCGC 2 cut(s) 474, 1164
Cfr13I GGNCC 5 cut(s) 13, 14, 550, 941, 1253
CseI GACGC 2 cut(s) 269, 943
Csp6I GTAC 4 cut(s) 223, 582, 789, 1179
CviAII CATG 4 cut(s) 70, 226, 782, 886
CviQI GTAC 4 cut(s) 223, 582, 789, 1179
DdeI CTNAG 4 cut(s) 213, 219, 477, 1143
DpnI GATC 1 cut(s) 1092
DpnII GATC 1 cut(s) 1090
DriI GACNNNNNGTC 1 cut(s) 939
EaeI YGGCCR 3 cut(s) 565, 637, 975
EagI CGGCCG 1 cut(s) 565
Eam1105I GACNNNNNGTC 1 cut(s) 939
Ecl136II GAGCTC 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 5
Eco24I GRGCYC 2 cut(s) 17, 418
Eco47I GGWCC 2 cut(s) 550, 941
Eco52I CGGCCG 1 cut(s) 565
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 738
Eco88I CYCGRG 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 416
EcoO109I RGGNCCY 2 cut(s) 941, 1253
EcoRI GAATTC 1 cut(s) 1120
EcoRII CCWGG 2 cut(s) 735, 1255
EcoT14I CCWWGG 1 cut(s) 5
EcoT38I GRGCYC 2 cut(s) 17, 418
ErhI CCWWGG 1 cut(s) 5
FaeI CATG 4 cut(s) 73, 229, 785, 889
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 2 cut(s) 260, 536
FatI CATG 4 cut(s) 69, 225, 781, 885
FauI CCCGC 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 161, 317, 333, 568
FriOI GRGCYC 2 cut(s) 17, 418
Fsp4HI GCNGC 4 cut(s) 161, 317, 333, 568
FspBI CTAG 5 cut(s) 6, 441, 498, 594, 1110
GlaI GCGC 2 cut(s) 473, 1163
GluI GCNGC 4 cut(s) 161, 317, 333, 568
GsaI CCCAGC 1 cut(s) 941
GsuI CTGGAG 2 cut(s) 339, 719
HaeII RGCGCY 1 cut(s) 475
HaeIII GGCC 7 cut(s) 10, 15, 22, 567, 639, 977, 1255
HapII CCGG 4 cut(s) 11, 37, 280, 896
HgaI GACGC 2 cut(s) 269, 943
HhaI GCGC 2 cut(s) 474, 1164
Hin1II CATG 4 cut(s) 73, 229, 785, 889
Hin6I GCGC 2 cut(s) 472, 1162
HinP1I GCGC 2 cut(s) 472, 1162
HincII GTYRAC 3 cut(s) 268, 663, 933
HindII GTYRAC 3 cut(s) 268, 663, 933
HindIII AAGCTT 1 cut(s) 970
HinfI GANTC 3 cut(s) 229, 557, 1100
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 4 cut(s) 11, 37, 280, 896
HphI GGTGA 2 cut(s) 707, 1035
Hpy166II GTNNAC 6 cut(s) 118, 268, 663, 933, 1104, 1236
Hpy188I TCNGA 7 cut(s) 214, 393, 603, 757, 1126, 1137, 1144
Hpy188III TCNNGA 4 cut(s) 233, 369, 419, 1013
Hpy8I GTNNAC 6 cut(s) 118, 268, 663, 933, 1104, 1236
HpyCH4III ACNGT 2 cut(s) 386, 660
HpyCH4V TGCA 4 cut(s) 351, 518, 544, 617
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 157, 260, 316, 823
HpyF3I CTNAG 4 cut(s) 213, 219, 477, 1143
Hsp92II CATG 4 cut(s) 73, 229, 785, 889
HspAI GCGC 2 cut(s) 472, 1162
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 1 cut(s) 1090
LmnI GCTCC 4 cut(s) 128, 307, 738, 814
Lsp1109I GCAGC 2 cut(s) 147, 319
LweI GCATC 3 cut(s) 437, 499, 579
MaeI CTAG 5 cut(s) 6, 441, 498, 594, 1110
MaeIII GTNAC 4 cut(s) 127, 398, 799, 861
MalI GATC 1 cut(s) 1092
MbiI CCGCTC 1 cut(s) 1131
MboI GATC 1 cut(s) 1090
MboII GAAGA 5 cut(s) 145, 148, 1001, 1032, 1202
MfeI CAATTG 2 cut(s) 506, 795
MhlI GDGCHC 3 cut(s) 17, 296, 418
MlsI TGGCCA 2 cut(s) 639, 977
MluCI AATT 9 cut(s) 189, 208, 506, 604, 625, 795, 1006, 1120, 1199
MluNI TGGCCA 2 cut(s) 639, 977
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 1 cut(s) 1115
Mox20I TGGCCA 2 cut(s) 639, 977
MroXI GAANNNNTTC 1 cut(s) 833
MscI TGGCCA 2 cut(s) 639, 977
MseI TTAA 4 cut(s) 176, 267, 339, 1028
Msp20I TGGCCA 2 cut(s) 639, 977
MspI CCGG 4 cut(s) 11, 37, 280, 896
MspR9I CCNGG 4 cut(s) 12, 737, 896, 1257
MunI CAATTG 2 cut(s) 506, 795
MvaI CCWGG 2 cut(s) 737, 1257
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 5 cut(s) 46, 157, 260, 316, 823
NciI CCSGG 2 cut(s) 12, 896
NdeII GATC 1 cut(s) 1090
NlaIII CATG 4 cut(s) 73, 229, 785, 889
NlaIV GGNNCC 7 cut(s) 15, 62, 79, 552, 816, 942, 1170
NmuCI GTSAC 1 cut(s) 861
NspI RCATGY 1 cut(s) 785
PciI ACATGT 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 2 cut(s) 229, 557
PkrI GCNGC 4 cut(s) 162, 318, 334, 569
PleI GAGTC 1 cut(s) 1108
PpsI GAGTC 1 cut(s) 1108
PpuMI RGGWCCY 1 cut(s) 941
PscI ACATGT 1 cut(s) 781
Psp124BI GAGCTC 1 cut(s) 418
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 2 cut(s) 735, 1255
PspFI CCCAGC 1 cut(s) 937
PspGI CCWGG 2 cut(s) 735, 1255
PspN4I GGNNCC 7 cut(s) 15, 62, 79, 552, 816, 942, 1170
PspOMI GGGCCC 1 cut(s) 13
PspPI GGNCC 5 cut(s) 13, 14, 550, 941, 1253
PspPPI RGGWCCY 1 cut(s) 941
PstNI CAGNNNCTG 1 cut(s) 1256
RsaI GTAC 4 cut(s) 224, 583, 790, 1180
RsaNI GTAC 4 cut(s) 223, 582, 789, 1179
SacI GAGCTC 1 cut(s) 418
SaqAI TTAA 4 cut(s) 176, 267, 339, 1028
SatI GCNGC 4 cut(s) 161, 317, 333, 568
Sau3AI GATC 1 cut(s) 1090
Sau96I GGNCC 5 cut(s) 13, 14, 550, 941, 1253
ScaI AGTACT 1 cut(s) 583
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 4 cut(s) 12, 737, 896, 1257
SduI GDGCHC 3 cut(s) 17, 296, 418
SfaNI GCATC 3 cut(s) 437, 499, 579
SfcI CTRYAG 2 cut(s) 459, 1248
SinI GGWCC 2 cut(s) 550, 941
SmlI CTYRAG 4 cut(s) 417, 620, 843, 1011
SmoI CTYRAG 4 cut(s) 417, 620, 843, 1011
Sse9I AATT 9 cut(s) 189, 208, 506, 604, 625, 795, 1006, 1120, 1199
SsiI CCGC 6 cut(s) 263, 317, 426, 488, 568, 1131
SspMI CTAG 5 cut(s) 6, 441, 498, 594, 1110
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 4 cut(s) 10, 735, 894, 1255
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 2 cut(s) 386, 660
TaqI TCGA 3 cut(s) 234, 368, 1243
TaqII GACCGA 1 cut(s) 731
TasI AATT 9 cut(s) 189, 208, 506, 604, 625, 795, 1006, 1120, 1199
TatI WGTACW 2 cut(s) 222, 581
TauI GCSGC 2 cut(s) 319, 570
TfiI GAWTC 2 cut(s) 229, 557
Tru1I TTAA 4 cut(s) 176, 267, 339, 1028
Tru9I TTAA 4 cut(s) 176, 267, 339, 1028
TscAI CASTG 3 cut(s) 301, 663, 985
TseFI GTSAC 1 cut(s) 861
TseI GCWGC 2 cut(s) 160, 332
Tsp45I GTSAC 1 cut(s) 861
TspDTI ATGAA 2 cut(s) 242, 480
TspGWI ACGGA 3 cut(s) 115, 882, 929
TspRI CASTG 3 cut(s) 301, 663, 985
VpaK11BI GGWCC 2 cut(s) 550, 941
XapI RAATTY 3 cut(s) 625, 1006, 1120
XceI RCATGY 1 cut(s) 785
XmaJI CCTAGG 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 5 cut(s) 6, 441, 498, 594, 1110
ZrmI AGTACT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.