Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0006246

Physical Location & Seq

Genomic Coordinates Reverse (-)

5023216 .. 5025800

Gene Structure 2585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1062700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGTATAATAATATCGGTTGTAATAAAAAAAAAATATTCTTCTTTGTCACGTGCGGACCTCCCGTTTCAGGAAAAGAAGGAAAAAAAACTTCGCACGTGTTTGCTCGCGCATCTCTTCATCTTCTTCTCCCTCTCCGTCCCTGTCTCTCTCTGCTTCTCTCTCGCCCGATTAATATGGACTCTCCACCAGCGAAGAGCTGGAGCATCCACAGCCGACCCGAAATCATCGCAAAATACAAGATCCTGGAGCGGGTCGGATCCGGAGCCTACTCCGATGTCTATAGAGCCATCCGCCTCTCCGACAACCTCACCGTCGCGCTCAAGGAGGTCCACGACTACCAGTCGGCGTTCCGAGAAATCGAGGCCCTCCAGGCCCTCCACAGCTGCCCCAACGTCATCGTTTTGTACGAGTACTTTTGGCGCGAGGACGAGGACGCCGTGCTCGTCCTCGAATTCCTGACCACTGACCTCGCCACTGTGATCAAAAGCGCCAAGAAACGGGAGGGTGGGATTGGGAGCGGCGAGGTCAAGCGGTGGATGCTGCAAATCCTATCGGGGGTTGACTCATGTCATCGGAATATGGTGGTCCACCGCGATTTGAAGCCCTGTAATTTGTTGATTGGGGACGATGGAGTTCTCAAGCTGGCCGATTTTGGTCAGGCTAGGATACTCTTGGAGGCGGGATATGTTCCTGATGAAAACCCACAACTGTATGAACGAAGCGCTCCCAGTCAGAGTGCCTTTCAGCCACCTGAAGCGATTCCCGAAAAAGATGAGTCATGTGAAGAAGGGTACAGAGCTCAAGAACAGGGAACTATGAGCAAAGAGGAATATTTTCGAGTGTTAGATGAGGTCAAGGCAAAAGGTTTGGATAAAGATACAAACGTTCCTGATGGAGATACATCTTGTATAGCAACATGTACAGCAAGCGACATAGACGATGATCTTTTCAGGGGTTCGTATTCTTATGAGGCAGAGGGCGGAAATGACAGAAATGGAGCTCTGACATCCTGTGTTGGGACTCGATGGTTCAGAGCTCCGGAACTACTCTACGGATCCATAGACTATGGTTTAGAGATAGATCTATGGTCGTTGGGCTGCATTTTTGCCGAGCTTTTGACGCTAGAGCCACTTTTTCCTGGAACTGCTGATATCGACCAGCTCAGCAGAATCATCAGTGTTCTGGGCAACTTAACTGAGGAGGCGTGGCCGGGGTGTCTTAAACTGCCTGATTACAGGATAATATCGTTCAATAAAGTAGAAAATCCAGTCGGCATAGAGGCCTGCCTGACCAACTGTTCCCCTGCCGAAGTCTCTCTGGTGAAGAAACTTATTTGTTACGACCCAGCTAAGAGAGCTACGGCTATGGAGCTCCTGCAGGACAAGTATTTCAGTGAAGAGCCTCTTCCAGTTCCTCTATCCGAGCTGCACGTCCCTGAGACCAAAGATATGGGTTCCGCCTCTCCTGGTGACTGGCCCGATTATGAAGATATGGGTTCCGACTCTGATTTTGACGACTTTCGCCCTGTAAATACTACCTCAACTGGTTCTCACATTCAGTCTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000079 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008047 GO:0008150 GO:0008152 GO:0009653 GO:0009888 GO:0009893 GO:0009987 GO:0010015 GO:0010073 GO:0010074 GO:0010078 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019827 GO:0019887 GO:0019912 GO:0022622 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033674 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0080090 GO:0097472 GO:0098727 GO:0098772 GO:0099402 GO:0140096 GO:1901564 GO:1904029 GO:1904031 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

523

Amino Acids

58.04

Weight (kDa)

4.8

Isoelectric Point (pI)

48.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	79 - 254	2.1e-32	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	80 - 230	4.1e-16	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	334 - 464	1e-19	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006246)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28980 AT4G28980 AT4G28980
fragaria_vesca	FvH4_1g05460
malus_domestica	MD02G1062700.v1.1 MD15G1190500.v1.1
prunus_persica	Prupe.7G226600_v2.0.a1
pyrus_communis	pycom02g04310 pycom02g04320 pycom15g17070
rosa_chinensis	RchiOBHm_Chr2g0091141
rosa_laevigata	RLG00000006226 RLG00000014960 RLG00000016185
rosa_multiflora	Rmu_co8390673.1_g000001 Rmu_sc0002343.1_g000049 Rmu_sc0004851.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150500 Rroxscaffold_5G00373520 Rroxscaffold_6G00397830 Rroxscaffold_7G00210230
rosa_rugosa	Rorug02G0013600
rosa_samantha	Rh2AG059600 Rh2BG058300 Rh2CG060100 Rh2DG058900
rosa_wichuraiana	Rw2G005030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	250, 519
AccII	CGCG	4 cut(s)	108, 317, 423, 594
AccIII	TCCGGA	2 cut(s)	260, 1039
AciI	CCGC	9 cut(s)	54, 250, 292, 519, 532, 592, 680, 981, 1458
AclI	AACGTT	1 cut(s)	885
AclWI	GGATC	5 cut(s)	235, 252, 265, 1050, 1063
AcoI	YGGCCR	2 cut(s)	645, 1208
AcsI	RAATTY	1 cut(s)	452
AcuI	CTGAAG	1 cut(s)	774
AcvI	CACGTG	2 cut(s)	51, 97
AcyI	GRCGYC	1 cut(s)	435
AfaI	GTAC	4 cut(s)	407, 413, 794, 922
AfeI	AGCGCT	1 cut(s)	724
AfiI	CCNNNNNNNGG	5 cut(s)	68, 250, 498, 556, 1017
AflIII	ACRYGT	2 cut(s)	96, 917
AgsI	TTSAA	2 cut(s)	601, 1252
AhdI	GACNNNNNGTC	1 cut(s)	340
AjiI	CACGTC	1 cut(s)	1432
AjnI	CCWGG	4 cut(s)	243, 369, 1138, 1465
Alw21I	GWGCWC	5 cut(s)	444, 802, 1003, 1039, 1374
Alw26I	GTCTC	3 cut(s)	149, 1318, 1433
AlwI	GGATC	5 cut(s)	235, 252, 265, 1050, 1063
Aor13HI	TCCGGA	2 cut(s)	260, 1039
Aor51HI	AGCGCT	1 cut(s)	724
AoxI	GGCC	6 cut(s)	363, 372, 645, 1208, 1281, 1475
ApeKI	GCWGC	4 cut(s)	384, 541, 1098, 1426
ApoI	RAATTY	1 cut(s)	452
ArsI	GACNNNNNNTTYG	2 cut(s)	1493, 1525
AseI	ATTAAT	1 cut(s)	171
Asp700I	GAANNNNTTC	2 cut(s)	759, 834
AspLEI	GCGC	5 cut(s)	110, 319, 423, 491, 725
AspS9I	GGNCC	6 cut(s)	56, 328, 364, 373, 586, 1476
AsuC2I	CCSGG	1 cut(s)	1212
AsuHPI	GGTGA	3 cut(s)	301, 1333, 1481
AvaII	GGWCC	3 cut(s)	56, 328, 586
BaeI	ACNNNNGTAYC	2 cut(s)	870, 903
BamHI	GGATCC	2 cut(s)	257, 1055
BanII	GRGCYC	4 cut(s)	802, 1003, 1039, 1374
BbrPI	CACGTG	2 cut(s)	51, 97
Bbv12I	GWGCWC	5 cut(s)	444, 802, 1003, 1039, 1374
BbvI	GCAGC	4 cut(s)	371, 528, 1085, 1413
BccI	CCATC	4 cut(s)	296, 623, 887, 1020
BceAI	ACGGC	2 cut(s)	422, 1377
BciT130I	CCWGG	4 cut(s)	245, 371, 1140, 1467
BciVI	GTATCC	1 cut(s)	660
BclI	TGATCA	1 cut(s)	480
BcnI	CCSGG	1 cut(s)	1212
BcoDI	GTCTC	3 cut(s)	149, 1318, 1433
BfaI	CTAG	2 cut(s)	663, 1124
BfmI	CTRYAG	2 cut(s)	280, 1376
BfoI	RGCGCY	2 cut(s)	492, 726
BfuI	GTATCC	1 cut(s)	660
BglI	GCCNNNNNGGC	1 cut(s)	371
BglII	AGATCT	1 cut(s)	1081
BisI	GCNGC	5 cut(s)	385, 520, 542, 1099, 1427
BlpI	GCTNAGC	1 cut(s)	1163
BlsI	GCNGC	5 cut(s)	386, 521, 543, 1100, 1428
BmcAI	AGTACT	1 cut(s)	413
Bme1390I	CCNGG	5 cut(s)	245, 371, 1140, 1212, 1467
Bme18I	GGWCC	3 cut(s)	56, 328, 586
BmeRI	GACNNNNNGTC	1 cut(s)	340
BmgBI	CACGTC	1 cut(s)	1432
BmgT120I	GGNCC	6 cut(s)	56, 328, 364, 373, 586, 1476
BmiI	GGNNCC	5 cut(s)	259, 265, 1057, 1456, 1498
BmrFI	CCNGG	5 cut(s)	245, 371, 1140, 1212, 1467
BmrI	ACTGGG	1 cut(s)	723
BmsI	GCATC	3 cut(s)	119, 213, 528
BmuI	ACTGGG	1 cut(s)	723
BoxI	GACNNNNGTC	1 cut(s)	567
BpmI	CTGGAG	3 cut(s)	220, 266, 353
Bpu1102I	GCTNAGC	1 cut(s)	1163
BpuEI	CTTGAG	3 cut(s)	305, 623, 786
BpuMI	CCSGG	1 cut(s)	1212
BsaAI	YACGTR	2 cut(s)	51, 97
BsaHI	GRCGYC	1 cut(s)	435
BsaI	GGTCTC	1 cut(s)	1433
BsaJI	CCNNGG	1 cut(s)	1211
BsaWI	WCCGGW	2 cut(s)	260, 1039
Bsc4I	CCNNNNNNNGG	5 cut(s)	68, 250, 498, 556, 1017
Bse1I	ACTGG	6 cut(s)	340, 729, 1268, 1409, 1478, 1549
BseAI	TCCGGA	2 cut(s)	260, 1039
BseBI	CCWGG	4 cut(s)	245, 371, 1140, 1467
BseDI	CCNNGG	1 cut(s)	1211
BseGI	GGATG	4 cut(s)	204, 288, 543, 1007
BseLI	CCNNNNNNNGG	5 cut(s)	68, 250, 498, 556, 1017
BseMII	CTCAG	3 cut(s)	1177, 1188, 1428
BseNI	ACTGG	6 cut(s)	340, 729, 1268, 1409, 1478, 1549
BseRI	GAGGAG	1 cut(s)	1214
BseXI	GCAGC	4 cut(s)	371, 528, 1085, 1413
BseYI	CCCAGC	1 cut(s)	1345
BsgI	GTGCAG	1 cut(s)	1412
Bsh1236I	CGCG	4 cut(s)	108, 317, 423, 594
BshFI	GGCC	6 cut(s)	365, 374, 647, 1210, 1283, 1477
BsiHKAI	GWGCWC	5 cut(s)	444, 802, 1003, 1039, 1374
BsiSI	CCGG	3 cut(s)	261, 1040, 1211
BslFI	GGGAC	4 cut(s)	123, 638, 1033, 1418
BslI	CCNNNNNNNGG	5 cut(s)	68, 250, 498, 556, 1017
BsmAI	GTCTC	3 cut(s)	149, 1318, 1433
BsmFI	GGGAC	4 cut(s)	123, 638, 1033, 1418
BsnI	GGCC	6 cut(s)	365, 374, 647, 1210, 1283, 1477
Bso31I	GGTCTC	1 cut(s)	1433
Bsp1286I	GDGCHC	5 cut(s)	444, 802, 1003, 1039, 1374
Bsp13I	TCCGGA	2 cut(s)	260, 1039
Bsp1407I	TGTACA	1 cut(s)	920
Bsp143I	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
Bsp1720I	GCTNAGC	1 cut(s)	1163
BspACI	CCGC	9 cut(s)	54, 250, 292, 519, 532, 592, 680, 981, 1458
BspANI	GGCC	6 cut(s)	365, 374, 647, 1210, 1283, 1477
BspCNI	CTCAG	3 cut(s)	1176, 1189, 1429
BspEI	TCCGGA	2 cut(s)	260, 1039
BspFNI	CGCG	4 cut(s)	108, 317, 423, 594
BspLI	GGNNCC	5 cut(s)	259, 265, 1057, 1456, 1498
BspMAI	CTGCAG	1 cut(s)	1380
BspPI	GGATC	5 cut(s)	235, 252, 265, 1050, 1063
BspQI	GCTCTTC	2 cut(s)	188, 1392
BspTNI	GGTCTC	1 cut(s)	1433
BsrBI	CCGCTC	2 cut(s)	250, 519
BsrGI	TGTACA	1 cut(s)	920
BsrI	ACTGG	6 cut(s)	340, 729, 1268, 1409, 1478, 1549
BssECI	CCNNGG	1 cut(s)	1211
BssMI	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
BssNI	GRCGYC	1 cut(s)	435
Bst2UI	CCWGG	4 cut(s)	245, 371, 1140, 1467
Bst4CI	ACNGT	4 cut(s)	313, 478, 711, 1298
Bst6I	CTCTTC	4 cut(s)	120, 188, 1392, 1410
BstACI	GRCGYC	1 cut(s)	435
BstAUI	TGTACA	1 cut(s)	920
BstBAI	YACGTR	2 cut(s)	51, 97
BstC8I	GCNNGC	4 cut(s)	106, 645, 928, 1285
BstDEI	CTNAG	4 cut(s)	1163, 1197, 1350, 1437
BstF5I	GGATG	4 cut(s)	204, 288, 543, 1007
BstFNI	CGCG	4 cut(s)	108, 317, 423, 594
BstH2I	RGCGCY	2 cut(s)	492, 726
BstHHI	GCGC	5 cut(s)	110, 319, 423, 491, 725
BstKTI	GATC	6 cut(s)	243, 260, 483, 946, 1058, 1084
BstMAI	GTCTC	3 cut(s)	149, 1318, 1433
BstMBI	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
BstMWI	GCNNNNNNNGC	5 cut(s)	210, 371, 538, 1120, 1355
BstNI	CCWGG	4 cut(s)	245, 371, 1140, 1467
BstNSI	RCATGY	1 cut(s)	921
BstPAI	GACNNNNGTC	1 cut(s)	567
BstSCI	CCNGG	5 cut(s)	243, 369, 1138, 1210, 1465
BstSFI	CTRYAG	2 cut(s)	280, 1376
BstUI	CGCG	4 cut(s)	108, 317, 423, 594
BstV1I	GCAGC	4 cut(s)	371, 528, 1085, 1413
BstX2I	RGATCY	4 cut(s)	240, 257, 1055, 1081
BstXI	CCANNNNNNTGG	1 cut(s)	1450
BstYI	RGATCY	4 cut(s)	240, 257, 1055, 1081
BsuI	GTATCC	1 cut(s)	660
BsuRI	GGCC	6 cut(s)	365, 374, 647, 1210, 1283, 1477
BtgZI	GCGATG	1 cut(s)	211
BtrI	CACGTC	1 cut(s)	1432
BtsCI	GGATG	4 cut(s)	204, 288, 543, 1007
BtsIMutI	CAGTG	4 cut(s)	462, 474, 1183, 1399
Cac8I	GCNNGC	4 cut(s)	106, 645, 928, 1285
CfoI	GCGC	5 cut(s)	110, 319, 423, 491, 725
Cfr13I	GGNCC	6 cut(s)	56, 328, 364, 373, 586, 1476
CseI	GACGC	2 cut(s)	443, 1129
Csp6I	GTAC	4 cut(s)	406, 412, 793, 921
CviAII	CATG	3 cut(s)	567, 780, 918
CviQI	GTAC	4 cut(s)	406, 412, 793, 921
DdeI	CTNAG	4 cut(s)	1163, 1197, 1350, 1437
DpnI	GATC	6 cut(s)	242, 259, 482, 945, 1057, 1083
DpnII	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
DriI	GACNNNNNGTC	1 cut(s)	340
EaeI	YGGCCR	2 cut(s)	645, 1208
Eam1104I	CTCTTC	4 cut(s)	120, 188, 1392, 1410
Eam1105I	GACNNNNNGTC	1 cut(s)	340
EarI	CTCTTC	4 cut(s)	120, 188, 1392, 1410
EciI	GGCGGA	3 cut(s)	281, 996, 1447
Ecl136II	GAGCTC	4 cut(s)	800, 1001, 1037, 1372
Eco147I	AGGCCT	1 cut(s)	1283
Eco24I	GRGCYC	4 cut(s)	802, 1003, 1039, 1374
Eco31I	GGTCTC	1 cut(s)	1433
Eco32I	GATATC	1 cut(s)	1153
Eco47I	GGWCC	3 cut(s)	56, 328, 586
Eco47III	AGCGCT	1 cut(s)	724
Eco53kI	GAGCTC	4 cut(s)	800, 1001, 1037, 1372
Eco57I	CTGAAG	1 cut(s)	774
Eco72I	CACGTG	2 cut(s)	51, 97
EcoICRI	GAGCTC	4 cut(s)	800, 1001, 1037, 1372
EcoO109I	RGGNCCY	2 cut(s)	364, 373
EcoRI	GAATTC	1 cut(s)	452
EcoRII	CCWGG	4 cut(s)	243, 369, 1138, 1465
EcoRV	GATATC	1 cut(s)	1153
EcoT38I	GRGCYC	4 cut(s)	802, 1003, 1039, 1374
FaeI	CATG	3 cut(s)	570, 783, 921
FalI	AAGNNNNNCTT	2 cut(s)	1389, 1421
FaqI	GGGAC	4 cut(s)	123, 638, 1033, 1418
FatI	CATG	3 cut(s)	566, 779, 917
FauI	CCCGC	2 cut(s)	243, 673
FbaI	TGATCA	1 cut(s)	480
Fnu4HI	GCNGC	5 cut(s)	385, 520, 542, 1099, 1427
FokI	GGATG	4 cut(s)	191, 275, 550, 994
FriOI	GRGCYC	4 cut(s)	802, 1003, 1039, 1374
Fsp4HI	GCNGC	5 cut(s)	385, 520, 542, 1099, 1427
FspBI	CTAG	2 cut(s)	663, 1124
GlaI	GCGC	5 cut(s)	109, 318, 422, 490, 724
GluI	GCNGC	5 cut(s)	385, 520, 542, 1099, 1427
GsaI	CCCAGC	1 cut(s)	1349
GsuI	CTGGAG	3 cut(s)	220, 266, 353
HaeII	RGCGCY	2 cut(s)	492, 726
HaeIII	GGCC	6 cut(s)	365, 374, 647, 1210, 1283, 1477
HapII	CCGG	3 cut(s)	261, 1040, 1211
HgaI	GACGC	2 cut(s)	443, 1129
HhaI	GCGC	5 cut(s)	110, 319, 423, 491, 725
Hin1I	GRCGYC	1 cut(s)	435
Hin1II	CATG	3 cut(s)	570, 783, 921
Hin6I	GCGC	5 cut(s)	108, 317, 421, 489, 723
HinP1I	GCGC	5 cut(s)	108, 317, 421, 489, 723
HincII	GTYRAC	1 cut(s)	562
HindII	GTYRAC	1 cut(s)	562
HinfI	GANTC	7 cut(s)	179, 563, 760, 776, 1021, 1170, 1502
HpaII	CCGG	3 cut(s)	261, 1040, 1211
HphI	GGTGA	3 cut(s)	301, 1333, 1481
Hpy166II	GTNNAC	3 cut(s)	331, 562, 589
Hpy188III	TCNNGA	8 cut(s)	69, 261, 457, 692, 764, 803, 890, 1040
Hpy8I	GTNNAC	3 cut(s)	331, 562, 589
Hpy99I	CGWCG	1 cut(s)	317
HpyAV	CCTTC	2 cut(s)	71, 782
HpyCH4III	ACNGT	4 cut(s)	313, 478, 711, 1298
HpyCH4IV	ACGT	5 cut(s)	50, 96, 393, 885, 1431
HpyCH4V	TGCA	4 cut(s)	544, 1101, 1378, 1429
HpyF10VI	GCNNNNNNNGC	5 cut(s)	210, 371, 538, 1120, 1355
HpyF3I	CTNAG	4 cut(s)	1163, 1197, 1350, 1437
HpySE526I	ACGT	5 cut(s)	50, 96, 393, 885, 1431
Hsp92I	GRCGYC	1 cut(s)	435
Hsp92II	CATG	3 cut(s)	570, 783, 921
HspAI	GCGC	5 cut(s)	108, 317, 421, 489, 723
Kpn2I	TCCGGA	2 cut(s)	260, 1039
Ksp22I	TGATCA	1 cut(s)	480
Kzo9I	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
LguI	GCTCTTC	2 cut(s)	188, 1392
LmnI	GCTCC	9 cut(s)	201, 247, 263, 516, 730, 998, 1042, 1369, 1377
Lsp1109I	GCAGC	4 cut(s)	371, 528, 1085, 1413
LweI	GCATC	3 cut(s)	119, 213, 528
MaeI	CTAG	2 cut(s)	663, 1124
MaeII	ACGT	5 cut(s)	50, 96, 393, 885, 1431
MaeIII	GTNAC	3 cut(s)	46, 1337, 1469
MalI	GATC	6 cut(s)	242, 259, 482, 945, 1057, 1083
MbiI	CCGCTC	2 cut(s)	250, 519
MboI	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
MflI	RGATCY	4 cut(s)	240, 257, 1055, 1081
MhlI	GDGCHC	5 cut(s)	444, 802, 1003, 1039, 1374
MluCI	AATT	2 cut(s)	452, 610
MlyI	GAGTC	5 cut(s)	173, 557, 785, 1015, 1496
MmeI	TCCRAC	3 cut(s)	235, 324, 1524
MroI	TCCGGA	2 cut(s)	260, 1039
MroXI	GAANNNNTTC	2 cut(s)	759, 834
MseI	TTAA	3 cut(s)	171, 1193, 1221
MspA1I	CMGCKG	1 cut(s)	384
MspI	CCGG	3 cut(s)	261, 1040, 1211
MspR9I	CCNGG	5 cut(s)	245, 371, 1140, 1212, 1467
MvaI	CCWGG	4 cut(s)	245, 371, 1140, 1467
MvnI	CGCG	4 cut(s)	108, 317, 423, 594
MwoI	GCNNNNNNNGC	5 cut(s)	210, 371, 538, 1120, 1355
NciI	CCSGG	1 cut(s)	1212
NdeII	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
NlaIII	CATG	3 cut(s)	570, 783, 921
NlaIV	GGNNCC	5 cut(s)	259, 265, 1057, 1456, 1498
NmeAIII	GCCGAG	1 cut(s)	1135
NmuCI	GTSAC	2 cut(s)	46, 1469
NspI	RCATGY	1 cut(s)	921
PceI	AGGCCT	1 cut(s)	1283
PciI	ACATGT	1 cut(s)	917
PciSI	GCTCTTC	2 cut(s)	188, 1392
PcsI	WCGNNNNNNNCGW	3 cut(s)	405, 435, 441
PdmI	GAANNNNTTC	2 cut(s)	759, 834
PfeI	GAWTC	2 cut(s)	760, 1170
PfoI	TCCNGGA	2 cut(s)	243, 1138
PkrI	GCNGC	5 cut(s)	386, 521, 543, 1100, 1428
PleI	GAGTC	5 cut(s)	173, 557, 784, 1015, 1496
PmaCI	CACGTG	2 cut(s)	51, 97
PmlI	CACGTG	2 cut(s)	51, 97
PpsI	GAGTC	5 cut(s)	173, 557, 784, 1015, 1496
Ppu21I	YACGTR	2 cut(s)	51, 97
PscI	ACATGT	1 cut(s)	917
PshAI	GACNNNNGTC	1 cut(s)	567
PshBI	ATTAAT	1 cut(s)	171
Psp124BI	GAGCTC	4 cut(s)	802, 1003, 1039, 1374
Psp1406I	AACGTT	1 cut(s)	885
Psp6I	CCWGG	4 cut(s)	243, 369, 1138, 1465
PspCI	CACGTG	2 cut(s)	51, 97
PspFI	CCCAGC	1 cut(s)	1345
PspGI	CCWGG	4 cut(s)	243, 369, 1138, 1465
PspN4I	GGNNCC	5 cut(s)	259, 265, 1057, 1456, 1498
PspPI	GGNCC	6 cut(s)	56, 328, 364, 373, 586, 1476
PstI	CTGCAG	1 cut(s)	1380
PsuI	RGATCY	4 cut(s)	240, 257, 1055, 1081
PvuII	CAGCTG	1 cut(s)	384
RsaI	GTAC	4 cut(s)	407, 413, 794, 922
RsaNI	GTAC	4 cut(s)	406, 412, 793, 921
SacI	GAGCTC	4 cut(s)	802, 1003, 1039, 1374
SapI	GCTCTTC	2 cut(s)	188, 1392
SaqAI	TTAA	3 cut(s)	171, 1193, 1221
SatI	GCNGC	5 cut(s)	385, 520, 542, 1099, 1427
Sau3AI	GATC	6 cut(s)	240, 257, 480, 943, 1055, 1081
Sau96I	GGNCC	6 cut(s)	56, 328, 364, 373, 586, 1476
SbfI	CCTGCAGG	1 cut(s)	1380
ScaI	AGTACT	1 cut(s)	413
SchI	GAGTC	5 cut(s)	173, 557, 785, 1015, 1496
ScrFI	CCNGG	5 cut(s)	245, 371, 1140, 1212, 1467
SdaI	CCTGCAGG	1 cut(s)	1380
SduI	GDGCHC	5 cut(s)	444, 802, 1003, 1039, 1374
SfaNI	GCATC	3 cut(s)	119, 213, 528
SfcI	CTRYAG	2 cut(s)	280, 1376
SfiI	GGCCNNNNNGGCC	1 cut(s)	371
SinI	GGWCC	3 cut(s)	56, 328, 586
SmlI	CTYRAG	3 cut(s)	320, 638, 801
SmoI	CTYRAG	3 cut(s)	320, 638, 801
Sse8387I	CCTGCAGG	1 cut(s)	1380
Sse9I	AATT	2 cut(s)	452, 610
SseBI	AGGCCT	1 cut(s)	1283
SsiI	CCGC	9 cut(s)	54, 250, 292, 519, 532, 592, 680, 981, 1458
SspI	AATATT	2 cut(s)	36, 833
SspMI	CTAG	2 cut(s)	663, 1124
SstI	GAGCTC	4 cut(s)	802, 1003, 1039, 1374
StuI	AGGCCT	1 cut(s)	1283
StyD4I	CCNGG	5 cut(s)	243, 369, 1138, 1210, 1465
TaaI	ACNGT	4 cut(s)	313, 478, 711, 1298
TaiI	ACGT	5 cut(s)	53, 99, 396, 888, 1434
TaqI	TCGA	5 cut(s)	360, 450, 838, 1024, 1155
TasI	AATT	2 cut(s)	452, 610
TatI	WGTACW	2 cut(s)	411, 920
TauI	GCSGC	1 cut(s)	522
TfiI	GAWTC	2 cut(s)	760, 1170
Tru1I	TTAA	3 cut(s)	171, 1193, 1221
Tru9I	TTAA	3 cut(s)	171, 1193, 1221
TscAI	CASTG	4 cut(s)	469, 481, 1183, 1399
TseFI	GTSAC	2 cut(s)	46, 1469
TseI	GCWGC	4 cut(s)	384, 541, 1098, 1426
Tsp45I	GTSAC	2 cut(s)	46, 1469
TspDTI	ATGAA	4 cut(s)	107, 711, 729, 1500
TspGWI	ACGGA	2 cut(s)	125, 1068
TspRI	CASTG	4 cut(s)	469, 481, 1183, 1399
VpaK11BI	GGWCC	3 cut(s)	56, 328, 586
VspI	ATTAAT	1 cut(s)	171
XapI	RAATTY	1 cut(s)	452
XceI	RCATGY	1 cut(s)	921
XcmI	CCANNNNNNNNNTGG	1 cut(s)	195
XmnI	GAANNNNTTC	2 cut(s)	759, 834
XspI	CTAG	2 cut(s)	663, 1124
ZrmI	AGTACT	1 cut(s)	413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.