Rroxscaffold_5G00373520
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006246
Physical Location & Seq
Reverse (-)
54719765 .. 54719980
216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373520.1

Sequence Viewer

Length: 216 bp
ATGGCCAAGTACCGGATCCTCGAGCAGGTCGGATCCGGCACCTACTCCAACGTCTACCGCTCCCGCCATCTCGCCGACAACCTCGGTGTTGCTCTTAAGGAGGTCCACGATTACCGGTCGGCCTTCCGCGAAATCGAGGCCCTCCGGACCCTCCACGGCTGCCCCAACGTCGTCATTTTGTACGAATACTTCGGCGCGAGGACGAGGACGCCGTGA

Protein Analysis

71

Amino Acids

8.17

Weight (kDa)

9.3

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 66 1.2e-07 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 38
AccBSI CCGCTC 1 cut(s) 60
AccI GTMKAC 1 cut(s) 54
AccII CGCG 2 cut(s) 129, 197
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 3 cut(s) 58, 64, 127
AclWI GGATC 4 cut(s) 10, 23, 27, 40
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 209
AfaI GTAC 2 cut(s) 11, 182
AfiI CCNNNNNNNGG 2 cut(s) 12, 25
AflII CTTAAG 1 cut(s) 95
AgeI ACCGGT 1 cut(s) 114
AlwI GGATC 4 cut(s) 10, 23, 27, 40
Ama87I CYCGRG 1 cut(s) 20
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 3 cut(s) 3, 120, 138
ApeKI GCWGC 1 cut(s) 159
AsiGI ACCGGT 1 cut(s) 114
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 3 cut(s) 103, 139, 147
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 2 cut(s) 103, 147
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 2 cut(s) 15, 32
BanI GGYRCC 1 cut(s) 38
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbvI GCAGC 1 cut(s) 146
BccI CCATC 1 cut(s) 75
BceAI ACGGC 2 cut(s) 172, 196
BfrI CTTAAG 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 16
BisI GCNGC 1 cut(s) 160
BlsI GCNGC 1 cut(s) 161
Bme18I GGWCC 2 cut(s) 103, 147
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 3 cut(s) 103, 139, 147
BmiI GGNNCC 4 cut(s) 17, 34, 40, 149
BsaHI GRCGYC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 82, 154
BsaWI WCCGGW 3 cut(s) 12, 114, 144
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 25
Bse118I RCCGGY 1 cut(s) 114
BseAI TCCGGA 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 82, 154
BseLI CCNNNNNNNGG 2 cut(s) 12, 25
BseXI GCAGC 1 cut(s) 146
Bsh1236I CGCG 2 cut(s) 129, 197
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 5, 122, 140
BshNI GGYRCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 114
BsiEI CGRYCG 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 4 cut(s) 13, 36, 115, 145
BslI CCNNNNNNNGG 2 cut(s) 12, 25
BsnI GGCC 3 cut(s) 5, 122, 140
BsoBI CYCGRG 1 cut(s) 20
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 2 cut(s) 15, 32
BspACI CCGC 3 cut(s) 58, 64, 127
BspANI GGCC 3 cut(s) 5, 122, 140
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 2 cut(s) 129, 197
BspLI GGNNCC 4 cut(s) 17, 34, 40, 149
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 4 cut(s) 10, 23, 27, 40
BspT107I GGYRCC 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 2 cut(s) 82, 154
BssMI GATC 2 cut(s) 15, 32
BssNI GRCGYC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 209
BstAFI CTTAAG 1 cut(s) 95
BstDSI CCRYGG 1 cut(s) 154
BstENI CCTNNNNNAGG 1 cut(s) 23
BstFNI CGCG 2 cut(s) 129, 197
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 2 cut(s) 18, 35
BstMBI GATC 2 cut(s) 15, 32
BstMCI CGRYCG 1 cut(s) 119
BstUI CGCG 2 cut(s) 129, 197
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 2 cut(s) 15, 32
BstYI RGATCY 2 cut(s) 15, 32
BsuRI GGCC 3 cut(s) 5, 122, 140
BtgI CCRYGG 1 cut(s) 154
BveI ACCTGC 1 cut(s) 16
CfoI GCGC 1 cut(s) 197
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 3 cut(s) 103, 139, 147
Csp6I GTAC 2 cut(s) 10, 181
CspAI ACCGGT 1 cut(s) 114
CviJI RGCY 4 cut(s) 5, 122, 140, 159
CviKI_1 RGCY 4 cut(s) 5, 122, 140, 159
CviQI GTAC 2 cut(s) 10, 181
DpnI GATC 2 cut(s) 17, 34
DpnII GATC 2 cut(s) 15, 32
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 2 cut(s) 103, 147
Eco88I CYCGRG 1 cut(s) 20
EcoNI CCTNNNNNAGG 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 139
FauI CCCGC 1 cut(s) 71
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 160
Fsp4HI GCNGC 1 cut(s) 160
GlaI GCGC 1 cut(s) 196
GluI GCNGC 1 cut(s) 160
HaeIII GGCC 3 cut(s) 5, 122, 140
HapII CCGG 4 cut(s) 13, 36, 115, 145
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 209
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HpaII CCGG 4 cut(s) 13, 36, 115, 145
Hpy166II GTNNAC 2 cut(s) 55, 106
Hpy188I TCNGA 1 cut(s) 32
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 2 cut(s) 55, 106
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 133
HpyCH4IV ACGT 2 cut(s) 51, 168
HpySE526I ACGT 2 cut(s) 51, 168
Hsp92I GRCGYC 1 cut(s) 209
HspAI GCGC 1 cut(s) 195
Kpn2I TCCGGA 1 cut(s) 144
Kzo9I GATC 2 cut(s) 15, 32
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 5 cut(s) 11, 26, 49, 128, 158
Lsp1109I GCAGC 1 cut(s) 146
MaeII ACGT 2 cut(s) 51, 168
MalI GATC 2 cut(s) 17, 34
MbiI CCGCTC 1 cut(s) 60
MboI GATC 2 cut(s) 15, 32
MflI RGATCY 2 cut(s) 15, 32
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 10, 72
MnlI CCTC 8 cut(s) 29, 92, 94, 130, 152, 161, 192, 198
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 144
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 96
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 4 cut(s) 13, 36, 115, 145
MvnI CGCG 2 cut(s) 129, 197
NdeII GATC 2 cut(s) 15, 32
NlaIV GGNNCC 4 cut(s) 17, 34, 40, 149
PaeR7I CTCGAG 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PinAI ACCGGT 1 cut(s) 114
PkrI GCNGC 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 17, 34, 40, 149
PspPI GGNCC 3 cut(s) 103, 139, 147
PspXI VCTCGAGB 1 cut(s) 20
PsuI RGATCY 2 cut(s) 15, 32
RsaI GTAC 2 cut(s) 11, 182
RsaNI GTAC 2 cut(s) 10, 181
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 1 cut(s) 160
Sau3AI GATC 2 cut(s) 15, 32
Sau96I GGNCC 3 cut(s) 103, 139, 147
SetI ASST 6 cut(s) 30, 44, 54, 84, 105, 171
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 2 cut(s) 103, 147
SlaI CTCGAG 1 cut(s) 20
SmlI CTYRAG 2 cut(s) 20, 95
SmoI CTYRAG 2 cut(s) 20, 95
SsiI CCGC 3 cut(s) 58, 64, 127
TaiI ACGT 2 cut(s) 54, 171
TaqI TCGA 2 cut(s) 21, 135
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseI GCWGC 1 cut(s) 159
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 103, 147
XagI CCTNNNNNAGG 1 cut(s) 23
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.