Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005945

Physical Location & Seq

Genomic Coordinates Reverse (-)

5803108 .. 5807082

Gene Structure 3975 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1071600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2511 bp

ATGGATGCGAGCATAACATACTCACCGGATGTAGATTACATAGACACAGGGATAAACAAGAACATCTCTTCTGAATTTATGAATCCAAATAATACCGACCTCCCCCCCGCCCCTCTCTTAGACCTCAGGAGTTTTCCCAAGGAAACAAAAATTGCTACGTCTACCTTCTTGTATGGAAACTACGATGGAGAAAACAAGTTACCGGAATTTGATCTCTACCTCGGCGTCAACTTTTGGTCGACGGTGAGGTTTGAAAATGCTTCAGATATTGTTACTAAGGAGATTATAGGTTTTGCAGAATCAAATACCGTGGACATTTGTCTAATAAATAAAGGGTTGGGAACTCCTTTCATTTCGGCGTTGGAACTTAGACCGCTTAATAGCTCAACTTATGGTACTGAATTTGGCACTTCTGCATCACTAGTACTCGTTGAAAGACTGGACATCGGACAAGGCAATGGAACAGGTCGATATGAAGATGATGTTTATGATCGCATTTGGTCACGTCACATTTCACCTTCTTGGGATCCAGTCAGTACTTCTTCGTTGATTACTACCTATGAGAACGGATATGAAGCACCATTGGAAGTAATCCAGACTGCAGCTGCACCTCAGAATCCAAGTGAACCTTTAAATTTGTACTGGAAAACAACTGACATGAATGCTCAGTTTTATGTTTTTATGTACTTTGCTGAAGTGGAGAATCTTGAGAAGAATCAGTCAAGAAAATTCAATATATCATGGAATGGATCTCCATTGGTTGGACGATTCACTCCCCGTTACTTACAGGCAGACGTGATATCTAATTCAAGAGCTCTGGTGGGAAAGGATCACGAAATTTCTTTACACAAGACAGAGGACTCTTCCCTTCCGCCGATTCTGAATGCTGTTGAAGTTTTCCAAGTTATGCAGCTAGTTCAATCACCTACTTATGGTGAAGATGCTGATGCCATAATGAACGTCAAAACTACATATCAAATAAAAAAAATCTGGGCTGGTGATCCTTGTGGACCTAGGAATTTCTCTTGGGAAGGACTAGAATGCAACTACAGCATTTTGGTTCCTCGGATTATTTCCCTAAACTTGAGTTCAAGCAGTTTGAGTGGGATAATAGCTGCTTCCATTGCCAATCTCTCGTCACTGGAGTCCTTAGATTTGTCGAACAATAACCTAGCCGGGCCAATGCCTCAGTTCTTGGAAGAACTAAAGTCGCTCACTCTCTTGAATTTGAAAGGCAATCAATTGTCTGGTTCGGTTCCAAACGCTCTTCGCAAGAGATCAGAAGCTGGATTACTTGTACTGAGTGTGGATACCGAAAATCTTTGTGGCTCAGGTTCATGCAAAAAGAAGAAGAAAATTGTTGCTCCAATAGTTGCGTCGTTTGTATCAGCACTAGTAGTCGTTATAGTGCTGATGCTAGTATGGAAATTGAGAAGAAAAAGAGAATCAGGCACAGAAGCAGAACCCTTTAACAAAACAGCTATAGCATCAACGAAGTGGCAGTTTACTCATGAGGAGGTATTAGATATCACCAAAAACTTACAAACCTCGATCGGGAAAGGAGGATTTGGGACTGTGTACCATGGTTGCATGAAAGACGGAACCCAAGTTGCGGTAAAAATGCTTTCTGCATCATCAACTCAAGGACCTAGGGAGTTCCAAACAGAGGCTGAGCTCCTGATGAGAATCTATCATAGAAACTTGGCCTCGTTTATTGGATACTGCGATGATGCAAACAACTTGGCGCTTATATACGAGTACATGGCTAATGGCAACCTAAAAAACTATCTCTCAGATGCAGAAAGAAGTTCACGGATGACATGGGAAATGAGACTTCGTATAGCAATTGATGCTGCACAAGGTCTCGAGTACTTGCATCACGGATGCAAGCCGCCTATTGTCCACAGAGATGTGAAGACAGCCAACATTCTCTTAAGCGAAAACTTGGAAGCTAAAATAGCGGATTTTGGTCTTTCCATGGTTTTTTCTAGTGACATTGAGACTAAAGTGGTCAGCACAGTCATGGGAACAGTCGGATATCTTGATCCAGAATACCACAATTGCCAGAGGCTGAATGAAAAGAGTGATGTGTATAGTTTTGGGGTAGTTCTATTAGAGCTAATCACCGGCCAACCTGCAGTGATAAAAAGTGATGAAGTAATCCACTTAGCAACGTGGGTGAGTTCTGAGCTCGAAAAAAGGGAACTAAAATGTATTGTAGATCAAAGGATGCTAGGGGAGTTCGATGAGCATTCGGTCTGGAAAGCGTTAGAGATAGCAATGGCGTGCACAACCTCTACCTCCCAACACAGAGTCACAATGGATGTTGTATTGTCTGAGCTCAAAAATTGCTTGGAAATGGAATTGTCTAGGCATCGAGAAAGAACTCCAACATCAACAGAAGAACTTCGCCCTGCGTTTGGGCCATATAATCATGGTTCACCTGAAGGTCTCACCATGTATACAGATTCCACAAATGTTGATTCCATGACCAGACCGTTTCCTAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

837

Amino Acids

93.09

Weight (kDa)

5.19

Isoelectric Point (pI)

37.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	3 - 302	3e-89	Malectin-like domain
LRR_8	PF13855	359 - 415	1.4e-08	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	514 - 780	3.2e-48	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	515 - 778	1.4e-43	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29180 AT4G29180 AT4G29180 AT4G29450
fragaria_vesca	FvH4_1g06670 FvH4_1g06670
malus_domestica	MD02G1069800.v1.1 MD02G1071600.v1.1 MD02G1071800.v1.1
prunus_persica	Prupe.7G215900_v2.0.a1
pyrus_communis	pycom02g05560 pycom02g05570 pycom15g17770 pycom15g17780
rosa_chinensis	RchiOBHm_Chr2g0092421 RchiOBHm_Chr2g0092431
rosa_laevigata	RLG00000016310
rosa_multiflora	Rmu_co8303041.1_g000001 Rmu_sc0002503.1_g000001 Rmu_sc0002503.1_g000003 Rmu_sc0013752.1_g000002
rosa_roxburghii	Rroxscaffold_2G00149130
rosa_rugosa	Rorug02G0026200.1
rosa_samantha	Rh2AG072000 Rh2BG072500 Rh2CG073700
rosa_wichuraiana	Rw2G006060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2143
AccB7I	CCANNNNNTGG	1 cut(s)	761
AccI	GTMKAC	3 cut(s)	161, 239, 2462
AciI	CCGC	6 cut(s)	108, 374, 872, 1613, 1892, 1961
AclWI	GGATC	6 cut(s)	521, 534, 757, 837, 995, 2039
AcoI	YGGCCR	1 cut(s)	2128
AcsI	RAATTY	8 cut(s)	74, 206, 401, 634, 728, 837, 1018, 1225
AcuI	CTGAAG	3 cut(s)	246, 714, 2466
AcyI	GRCGYC	1 cut(s)	225
AfaI	GTAC	9 cut(s)	397, 426, 538, 641, 686, 1299, 1580, 1760, 1871
AfiI	CCNNNNNNNGG	6 cut(s)	761, 932, 1554, 1612, 1666, 2420
AflII	CTTAAG	1 cut(s)	1933
AgsI	TTSAA	9 cut(s)	254, 434, 733, 810, 893, 920, 1092, 1225, 1231
AhlI	ACTAGT	2 cut(s)	421, 1393
AjiI	CACGTC	2 cut(s)	506, 796
Alw21I	GWGCWC	5 cut(s)	817, 1677, 2193, 2291, 2343
Alw26I	GTCTC	4 cut(s)	1825, 1868, 1994, 2456
Alw44I	GTGCAC	1 cut(s)	2287
AlwI	GGATC	6 cut(s)	521, 534, 757, 837, 995, 2039
AlwNI	CAGNNNCTG	3 cut(s)	1286, 1670, 2071
Ama87I	CYCGRG	1 cut(s)	1865
AoxI	GGCC	4 cut(s)	1178, 1704, 2128, 2423
ApaLI	GTGCAC	1 cut(s)	2287
ApeKI	GCWGC	5 cut(s)	602, 605, 910, 1115, 1853
ApoI	RAATTY	8 cut(s)	74, 206, 401, 634, 728, 837, 1018, 1225
Asp700I	GAANNNNTTC	1 cut(s)	2406
AspA2I	CCTAGG	3 cut(s)	1013, 1649, 2504
AspLEI	GCGC	1 cut(s)	1747
AspS9I	GGNCC	4 cut(s)	1010, 1178, 1646, 2423
AsuC2I	CCSGG	1 cut(s)	1177
AvaI	CYCGRG	1 cut(s)	1865
AvaII	GGWCC	2 cut(s)	1010, 1646
AvrII	CCTAGG	3 cut(s)	1013, 1649, 2504
AxyI	CCTNAGG	1 cut(s)	125
BaeGI	GKGCMC	1 cut(s)	2291
BamHI	GGATCC	1 cut(s)	526
BanII	GRGCYC	4 cut(s)	817, 1677, 2193, 2343
BarI	GAAGNNNNNNTAC	2 cut(s)	1275, 1307
BbsI	GAAGAC	1 cut(s)	1922
Bbv12I	GWGCWC	5 cut(s)	817, 1677, 2193, 2291, 2343
BbvI	GCAGC	5 cut(s)	592, 614, 922, 1102, 1840
BccI	CCATC	1 cut(s)	179
BciVI	GTATCC	2 cut(s)	1303, 1712
BcnI	CCSGG	1 cut(s)	1177
BcoDI	GTCTC	4 cut(s)	1825, 1868, 1994, 2456
BcuI	ACTAGT	2 cut(s)	421, 1393
BfmI	CTRYAG	4 cut(s)	600, 1048, 1482, 2136
BfoI	RGCGCY	1 cut(s)	1748
BfrI	CTTAAG	1 cut(s)	1933
BfuAI	ACCTGC	1 cut(s)	2143
BfuI	GTATCC	2 cut(s)	1303, 1712
BisI	GCNGC	6 cut(s)	603, 606, 911, 1116, 1854, 1892
BlnI	CCTAGG	3 cut(s)	1013, 1649, 2504
BlpI	GCTNAGC	1 cut(s)	1671
BlsI	GCNGC	6 cut(s)	604, 607, 912, 1117, 1855, 1893
BmcAI	AGTACT	3 cut(s)	426, 538, 1871
Bme1390I	CCNGG	1 cut(s)	1177
Bme18I	GGWCC	2 cut(s)	1010, 1646
BmeT110I	CYCGRG	1 cut(s)	1865
BmgBI	CACGTC	2 cut(s)	506, 796
BmgT120I	GGNCC	4 cut(s)	1010, 1178, 1646, 2423
BmiI	GGNNCC	4 cut(s)	528, 1062, 1257, 1603
BmrFI	CCNGG	1 cut(s)	1177
BoxI	GACNNNNGTC	1 cut(s)	318
BpiI	GAAGAC	1 cut(s)	1922
BpmI	CTGGAG	1 cut(s)	1163
Bpu10I	CCTNAGC	1 cut(s)	1330
Bpu1102I	GCTNAGC	1 cut(s)	1671
BpuEI	CTTGAG	3 cut(s)	728, 1105, 1626
BpuMI	CCSGG	1 cut(s)	1177
BsaBI	GATNNNNATC	1 cut(s)	1685
BsaHI	GRCGYC	1 cut(s)	225
BsaI	GGTCTC	2 cut(s)	1868, 2456
BsaJI	CCNNGG	9 cut(s)	138, 220, 309, 1013, 1064, 1582, 1649, 1977, 2504
BsaWI	WCCGGW	2 cut(s)	25, 202
Bsc4I	CCNNNNNNNGG	6 cut(s)	761, 932, 1554, 1612, 1666, 2420
Bse118I	RCCGGY	1 cut(s)	2126
Bse1I	ACTGG	4 cut(s)	444, 530, 647, 1146
Bse21I	CCTNAGG	1 cut(s)	125
Bse3DI	GCAATG	3 cut(s)	463, 1122, 2286
Bse8I	GATNNNNATC	1 cut(s)	1685
BseDI	CCNNGG	9 cut(s)	138, 220, 309, 1013, 1064, 1582, 1649, 1977, 2504
BseGI	GGATG	6 cut(s)	10, 34, 1821, 1889, 2235, 2329
BseJI	GATNNNNATC	1 cut(s)	1685
BseLI	CCNNNNNNNGG	6 cut(s)	761, 932, 1554, 1612, 1666, 2420
BseMI	GCAATG	3 cut(s)	463, 1122, 2286
BseNI	ACTGG	4 cut(s)	444, 530, 647, 1146
BseRI	GAGGAG	1 cut(s)	1529
BseSI	GKGCMC	1 cut(s)	2291
BseXI	GCAGC	5 cut(s)	592, 614, 922, 1102, 1840
BsgI	GTGCAG	2 cut(s)	591, 1839
Bsh1285I	CGRYCG	1 cut(s)	1554
BshFI	GGCC	4 cut(s)	1180, 1706, 2130, 2425
BsiEI	CGRYCG	1 cut(s)	1554
BsiHKAI	GWGCWC	5 cut(s)	817, 1677, 2193, 2291, 2343
BsiHKCI	CYCGRG	1 cut(s)	1865
BsiSI	CCGG	4 cut(s)	26, 203, 1176, 2127
BslFI	GGGAC	1 cut(s)	1585
BslI	CCNNNNNNNGG	6 cut(s)	761, 932, 1554, 1612, 1666, 2420
BsmAI	GTCTC	4 cut(s)	1825, 1868, 1994, 2456
BsmFI	GGGAC	1 cut(s)	1585
BsmI	GAATGC	4 cut(s)	667, 889, 1046, 2251
BsnI	GGCC	4 cut(s)	1180, 1706, 2130, 2425
Bso31I	GGTCTC	2 cut(s)	1868, 2456
BsoBI	CYCGRG	1 cut(s)	1865
Bsp1286I	GDGCHC	5 cut(s)	817, 1677, 2193, 2291, 2343
Bsp1720I	GCTNAGC	1 cut(s)	1671
Bsp19I	CCATGG	2 cut(s)	1582, 1977
BspACI	CCGC	6 cut(s)	108, 374, 872, 1613, 1892, 1961
BspANI	GGCC	4 cut(s)	1180, 1706, 2130, 2425
BspHI	TCATGA	1 cut(s)	1510
BspLI	GGNNCC	4 cut(s)	528, 1062, 1257, 1603
BspMAI	CTGCAG	2 cut(s)	604, 2140
BspMI	ACCTGC	1 cut(s)	2143
BspPI	GGATC	6 cut(s)	521, 534, 757, 837, 995, 2039
BspQI	GCTCTTC	1 cut(s)	1272
BspTI	CTTAAG	1 cut(s)	1933
BspTNI	GGTCTC	2 cut(s)	1868, 2456
BsrDI	GCAATG	3 cut(s)	463, 1122, 2286
BsrFI	RCCGGY	1 cut(s)	2126
BsrI	ACTGG	4 cut(s)	444, 530, 647, 1146
BssAI	RCCGGY	1 cut(s)	2126
BssECI	CCNNGG	9 cut(s)	138, 220, 309, 1013, 1064, 1582, 1649, 1977, 2504
BssNAI	GTATAC	1 cut(s)	2463
BssNI	GRCGYC	1 cut(s)	225
BssT1I	CCWWGG	6 cut(s)	138, 1013, 1582, 1649, 1977, 2504
Bst1107I	GTATAC	1 cut(s)	2463
Bst4CI	ACNGT	6 cut(s)	244, 310, 1576, 2020, 2032, 2499
Bst6I	CTCTTC	3 cut(s)	73, 868, 1272
BstACI	GRCGYC	1 cut(s)	225
BstAFI	CTTAAG	1 cut(s)	1933
BstAPI	GCANNNNNTGC	1 cut(s)	1850
BstC8I	GCNNGC	3 cut(s)	10, 1889, 2287
BstDSI	CCRYGG	3 cut(s)	309, 1582, 1977
BstF5I	GGATG	6 cut(s)	10, 34, 1821, 1889, 2235, 2329
BstH2I	RGCGCY	1 cut(s)	1748
BstHHI	GCGC	1 cut(s)	1747
BstMAI	GTCTC	4 cut(s)	1825, 1868, 1994, 2456
BstMCI	CGRYCG	1 cut(s)	1554
BstMWI	GCNNNNNNNGC	4 cut(s)	1050, 1124, 1850, 1958
BstPAI	GACNNNNGTC	1 cut(s)	318
BstSCI	CCNGG	1 cut(s)	1175
BstSFI	CTRYAG	4 cut(s)	600, 1048, 1482, 2136
BstSLI	GKGCMC	1 cut(s)	2291
BstV1I	GCAGC	5 cut(s)	592, 614, 922, 1102, 1840
BstV2I	GAAGAC	1 cut(s)	1922
BstX2I	RGATCY	2 cut(s)	526, 749
BstYI	RGATCY	2 cut(s)	526, 749
BstZ17I	GTATAC	1 cut(s)	2463
Bsu36I	CCTNAGG	1 cut(s)	125
BsuI	GTATCC	2 cut(s)	1303, 1712
BsuRI	GGCC	4 cut(s)	1180, 1706, 2130, 2425
BtgI	CCRYGG	3 cut(s)	309, 1582, 1977
BtgZI	GCGATG	1 cut(s)	1740
BtrI	CACGTC	2 cut(s)	506, 796
BtsCI	GGATG	6 cut(s)	10, 34, 1821, 1889, 2235, 2329
BtsI	GCAGTG	1 cut(s)	2145
BtsIMutI	CAGTG	2 cut(s)	1139, 2145
BveI	ACCTGC	1 cut(s)	2143
Cac8I	GCNNGC	3 cut(s)	10, 1889, 2287
CaiI	CAGNNNCTG	3 cut(s)	1286, 1670, 2071
CciI	TCATGA	1 cut(s)	1510
CfoI	GCGC	1 cut(s)	1747
Cfr10I	RCCGGY	1 cut(s)	2126
Cfr13I	GGNCC	4 cut(s)	1010, 1178, 1646, 2423
CseI	GACGC	2 cut(s)	214, 1365
Csp6I	GTAC	9 cut(s)	396, 425, 537, 640, 685, 1298, 1579, 1759, 1870
CspCI	CAANNNNNGTGG	4 cut(s)	291, 326, 2461, 2496
CviQI	GTAC	9 cut(s)	396, 425, 537, 640, 685, 1298, 1579, 1759, 1870
DraI	TTTAAA	1 cut(s)	633
EaeI	YGGCCR	1 cut(s)	2128
Eam1104I	CTCTTC	3 cut(s)	73, 868, 1272
EarI	CTCTTC	3 cut(s)	73, 868, 1272
EciI	GGCGGA	1 cut(s)	861
Ecl136II	GAGCTC	4 cut(s)	815, 1675, 2191, 2341
Eco130I	CCWWGG	6 cut(s)	138, 1013, 1582, 1649, 1977, 2504
Eco24I	GRGCYC	4 cut(s)	817, 1677, 2193, 2343
Eco31I	GGTCTC	2 cut(s)	1868, 2456
Eco32I	GATATC	3 cut(s)	801, 1528, 2039
Eco47I	GGWCC	2 cut(s)	1010, 1646
Eco53kI	GAGCTC	4 cut(s)	815, 1675, 2191, 2341
Eco57I	CTGAAG	3 cut(s)	246, 714, 2466
Eco81I	CCTNAGG	1 cut(s)	125
Eco88I	CYCGRG	1 cut(s)	1865
EcoICRI	GAGCTC	4 cut(s)	815, 1675, 2191, 2341
EcoO109I	RGGNCCY	1 cut(s)	1646
EcoRV	GATATC	3 cut(s)	801, 1528, 2039
EcoT14I	CCWWGG	6 cut(s)	138, 1013, 1582, 1649, 1977, 2504
EcoT38I	GRGCYC	4 cut(s)	817, 1677, 2193, 2343
ErhI	CCWWGG	6 cut(s)	138, 1013, 1582, 1649, 1977, 2504
FalI	AAGNNNNNCTT	2 cut(s)	613, 645
FaqI	GGGAC	1 cut(s)	1585
FauI	CCCGC	1 cut(s)	115
FblI	GTMKAC	3 cut(s)	161, 239, 2462
Fnu4HI	GCNGC	6 cut(s)	603, 606, 911, 1116, 1854, 1892
FokI	GGATG	6 cut(s)	17, 41, 1828, 1896, 2242, 2336
FriOI	GRGCYC	4 cut(s)	817, 1677, 2193, 2343
Fsp4HI	GCNGC	6 cut(s)	603, 606, 911, 1116, 1854, 1892
GlaI	GCGC	1 cut(s)	1746
GluI	GCNGC	6 cut(s)	603, 606, 911, 1116, 1854, 1892
GsuI	CTGGAG	1 cut(s)	1163
HaeII	RGCGCY	1 cut(s)	1748
HaeIII	GGCC	4 cut(s)	1180, 1706, 2130, 2425
HapII	CCGG	4 cut(s)	26, 203, 1176, 2127
HgaI	GACGC	2 cut(s)	214, 1365
HhaI	GCGC	1 cut(s)	1747
Hin1I	GRCGYC	1 cut(s)	225
Hin6I	GCGC	1 cut(s)	1745
HinP1I	GCGC	1 cut(s)	1745
HincII	GTYRAC	2 cut(s)	229, 240
HindII	GTYRAC	2 cut(s)	229, 240
HpaII	CCGG	4 cut(s)	26, 203, 1176, 2127
Hpy99I	CGWCG	2 cut(s)	244, 1381
HpyAV	CCTTC	5 cut(s)	175, 528, 878, 1025, 2441
HpyCH4III	ACNGT	6 cut(s)	244, 310, 1576, 2020, 2032, 2499
HpyCH4IV	ACGT	5 cut(s)	158, 505, 795, 960, 2174
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1050, 1124, 1850, 1958
HpySE526I	ACGT	5 cut(s)	158, 505, 795, 960, 2174
Hsp92I	GRCGYC	1 cut(s)	225
HspAI	GCGC	1 cut(s)	1745
LguI	GCTCTTC	1 cut(s)	1272
LmnI	GCTCC	2 cut(s)	1369, 1680
Lsp1109I	GCAGC	5 cut(s)	592, 614, 922, 1102, 1840
MaeII	ACGT	5 cut(s)	158, 505, 795, 960, 2174
MaeIII	GTNAC	8 cut(s)	198, 271, 501, 506, 779, 1137, 1991, 2314
MfeI	CAATTG	3 cut(s)	1241, 1845, 2059
MflI	RGATCY	2 cut(s)	526, 749
MhlI	GDGCHC	5 cut(s)	817, 1677, 2193, 2291, 2343
MlyI	GAGTC	3 cut(s)	854, 1154, 2322
MmeI	TCCRAC	4 cut(s)	342, 742, 2014, 2414
MroXI	GAANNNNTTC	1 cut(s)	2406
MseI	TTAA	4 cut(s)	378, 632, 1470, 1934
MslI	CAYNNNNRTG	2 cut(s)	1908, 2021
MspA1I	CMGCKG	1 cut(s)	605
MspCI	CTTAAG	1 cut(s)	1933
MspI	CCGG	4 cut(s)	26, 203, 1176, 2127
MspR9I	CCNGG	1 cut(s)	1177
MunI	CAATTG	3 cut(s)	1241, 1845, 2059
Mva1269I	GAATGC	4 cut(s)	667, 889, 1046, 2251
MwoI	GCNNNNNNNGC	4 cut(s)	1050, 1124, 1850, 1958
NciI	CCSGG	1 cut(s)	1177
NcoI	CCATGG	2 cut(s)	1582, 1977
NlaIV	GGNNCC	4 cut(s)	528, 1062, 1257, 1603
NmeAIII	GCCGAG	1 cut(s)	201
NmuCI	GTSAC	5 cut(s)	501, 506, 1137, 1991, 2314
PaeR7I	CTCGAG	1 cut(s)	1865
PagI	TCATGA	1 cut(s)	1510
PciSI	GCTCTTC	1 cut(s)	1272
PctI	GAATGC	4 cut(s)	667, 889, 1046, 2251
PdmI	GAANNNNTTC	1 cut(s)	2406
PflMI	CCANNNNNTGG	1 cut(s)	761
PkrI	GCNGC	6 cut(s)	604, 607, 912, 1117, 1855, 1893
Ple19I	CGATCG	1 cut(s)	1554
PleI	GAGTC	3 cut(s)	854, 1153, 2321
PpsI	GAGTC	3 cut(s)	854, 1153, 2321
PpuMI	RGGWCCY	1 cut(s)	1646
PshAI	GACNNNNGTC	1 cut(s)	318
Psp124BI	GAGCTC	4 cut(s)	817, 1677, 2193, 2343
Psp5II	RGGWCCY	1 cut(s)	1646
PspN4I	GGNNCC	4 cut(s)	528, 1062, 1257, 1603
PspPI	GGNCC	4 cut(s)	1010, 1178, 1646, 2423
PspPPI	RGGWCCY	1 cut(s)	1646
PstI	CTGCAG	2 cut(s)	604, 2140
PstNI	CAGNNNCTG	3 cut(s)	1286, 1670, 2071
PsuI	RGATCY	2 cut(s)	526, 749
PvuI	CGATCG	1 cut(s)	1554
PvuII	CAGCTG	1 cut(s)	605
RsaI	GTAC	9 cut(s)	397, 426, 538, 641, 686, 1299, 1580, 1760, 1871
RsaNI	GTAC	9 cut(s)	396, 425, 537, 640, 685, 1298, 1579, 1759, 1870
RseI	CAYNNNNRTG	2 cut(s)	1908, 2021
SacI	GAGCTC	4 cut(s)	817, 1677, 2193, 2343
SalI	GTCGAC	1 cut(s)	238
SapI	GCTCTTC	1 cut(s)	1272
SaqAI	TTAA	4 cut(s)	378, 632, 1470, 1934
SatI	GCNGC	6 cut(s)	603, 606, 911, 1116, 1854, 1892
Sau96I	GGNCC	4 cut(s)	1010, 1178, 1646, 2423
ScaI	AGTACT	3 cut(s)	426, 538, 1871
SchI	GAGTC	3 cut(s)	854, 1154, 2322
ScrFI	CCNGG	1 cut(s)	1177
SduI	GDGCHC	5 cut(s)	817, 1677, 2193, 2291, 2343
SfcI	CTRYAG	4 cut(s)	600, 1048, 1482, 2136
Sfr274I	CTCGAG	1 cut(s)	1865
SinI	GGWCC	2 cut(s)	1010, 1646
SlaI	CTCGAG	1 cut(s)	1865
SmiMI	CAYNNNNRTG	2 cut(s)	1908, 2021
SmlI	CTYRAG	5 cut(s)	707, 1084, 1641, 1865, 1933
SmoI	CTYRAG	5 cut(s)	707, 1084, 1641, 1865, 1933
SpeI	ACTAGT	2 cut(s)	421, 1393
SsiI	CCGC	6 cut(s)	108, 374, 872, 1613, 1892, 1961
SstI	GAGCTC	4 cut(s)	817, 1677, 2193, 2343
StyD4I	CCNGG	1 cut(s)	1175
StyI	CCWWGG	6 cut(s)	138, 1013, 1582, 1649, 1977, 2504
TaaI	ACNGT	6 cut(s)	244, 310, 1576, 2020, 2032, 2499
TaiI	ACGT	5 cut(s)	161, 508, 798, 963, 2177
TaqI	TCGA	8 cut(s)	239, 469, 1160, 1550, 1866, 2193, 2244, 2377
TaqII	GACCGA	1 cut(s)	2245
TatI	WGTACW	7 cut(s)	424, 536, 639, 684, 1297, 1758, 1869
TauI	GCSGC	1 cut(s)	1894
Tru1I	TTAA	4 cut(s)	378, 632, 1470, 1934
Tru9I	TTAA	4 cut(s)	378, 632, 1470, 1934
TscAI	CASTG	2 cut(s)	1146, 2145
TseFI	GTSAC	5 cut(s)	501, 506, 1137, 1991, 2314
TseI	GCWGC	5 cut(s)	602, 605, 910, 1115, 1853
Tsp45I	GTSAC	5 cut(s)	501, 506, 1137, 1991, 2314
TspGWI	ACGGA	4 cut(s)	582, 1614, 1828, 1896
TspRI	CASTG	2 cut(s)	1146, 2145
Van91I	CCANNNNNTGG	1 cut(s)	761
Vha464I	CTTAAG	1 cut(s)	1933
VneI	GTGCAC	1 cut(s)	2287
VpaK11BI	GGWCC	2 cut(s)	1010, 1646
XapI	RAATTY	8 cut(s)	74, 206, 401, 634, 728, 837, 1018, 1225
XhoI	CTCGAG	1 cut(s)	1865
XmaJI	CCTAGG	3 cut(s)	1013, 1649, 2504
XmiI	GTMKAC	3 cut(s)	161, 239, 2462
XmnI	GAANNNNTTC	1 cut(s)	2406
ZrmI	AGTACT	3 cut(s)	426, 538, 1871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.