MD02G1071800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005945
Physical Location & Seq
Reverse (-)
5823462 .. 5827650
4189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1071800.v1.1.491

Sequence Viewer

Length: 2673 bp
ATGTCAACTGGGTTCTTCCTCCTCCTCCTTTTGATGCTCTGTGCAAGTTCAGTTCGTGCGCAGCGCCGGATAGACTTCATAAGCATAGATTGTGGTTCTCCCTCGAAGCTCTACGAAGACACGGATACGAACATAACATACTCACCGGATGGAGATTACATAGACACAGGGATAAACCACAACATCTCTTCTGAATTTAGTTATCCAAAGAATCCCAACCTCCCCTTCCCTCTCTCCGACCTCAGGAGTTTCCCTCAAGGAAACAAAAATTGCTATACGTTGAAACCCGAAGCAGGAAATGGCAGTTTGAATTTGATTAGAGCTACCTTCTTGTATGGAAACTACGATGGAGAAAACAAGTTGCCGGAATTTGATCTCTACCTCGGCGTCAACTTTTGGTCGACGGTGAGGTTTGAAAATGCTTCAGATATTGTTACCAAGGAGATTTTAGGTTTTGCAGAATCAAATACCGTGGACATTTGTCTAATAAATAAAGGGTTGGGAACTCCTTTCATTTCGGCGTTGGAATTTAGACCGCTTAATAGCTCAACTTATGGTACTGAATTTGGGACTTCTGCATCATTAGTACTCATTGAAAGACTGGACATTGGACAAGGCAATGGAACAGGTCGATATGGAGATGATGTTTATGATCGCATTTGGTCGCGTCACATTTCACCTTCTTGGGATCCAGTTAGTACTTCTTCGTCGATTACTACCTATGAGAACGGATATAGAGTGCCATTGGAAGTAATCCGGACTGCAGCTACACCTCAGAATGCAAGTGAACCTTTAGATTTGTACTGGAACACAACTGACATGAATGCTCAGTTTTATGTTTTTATGTACTTTGCTGAAGTGGAGAAACTTGGGAAGAATCAGTCAAGAAAATTCAATATATCATTGAATGGTTCTCCATTGTTTGGACCATTCACTCCCCGTTACTTACAGGCAGACGTGATATCTAATTCAAGAGTTCTGGTGGGAAAGGATCACGAAATTTCTTTACACAAGACAGAGGACTCTTCCCTTCCACCGATTCTGAATGCTGTTGAAGTTTTCCAAGTTATGCAGCTGGTTGAATCACCTACTTATGGTGAAGATGCTGATGCCATAAAGAACGTCAAAACTACATATCAAATAAAAAAAATCTGGGCTGGTGATCCTTGTGGACCTAGGAATTTCTCTTGGGAAGGACTAGAATGCAACTACAGCATTTCGCTTCCTCGGATTATTTCCCTAAACTTGAGTTCAAGCAATTTGAGTGGGATAATAGCTGCTTCCATTGCCAATCTCTCATCACTCGAGTCCTTAGATTTGTCGAACAATAACCTAACCGGGCCAGTGCCTCAGTTCTTGGAAGAACTAAAGTCGCTCACTCTCTTGAATTTGAAGGGCAATCAATTGTCTGGTTCGGTTCCAAACGCTCTTCGCGAGAGATCGGAAGCTGGATTACTTGTACTGAGTGTGGATACCAAAAATCTTTGTGGCTCGGGTTCATGCAAAAAGAAGAAGAAAATTGTTGCTCCAATAGTTGCGTCGTTAGTAGCAGCACTAGCAGTCGTTATAGTGCTGATGCTAGTATGGAAATTGAGAAGAAAAAGAGAATCAGGTACAGAATTAGAACCCTTCAACAAAACAGCTATAGCATCAAAGAAGTGCCAGTTTACTCATGAGGAGGTTTTAGATATCACCAAAAACTTACAAACCTCGATCGGGAAAGGAGGATTTGGGACTGTGTACCATGGTTGCATGAAAGACGGAACCCAAGTTGCGGTAAAAATGCTTTCTGCATCATCAACTCAAGGACCTAGGGAGTTCCAAACAGAGGCTGAGCTCCTGATGAGAATCCATCATAGAAACTTGGCCTCGTTTATTGGATACTGTGATGATGCGAACAACTTGGCACTTATATACGAGTACATGGCTAATGGCAACCTAAAAAACTCTCTCTCAGATGCAGAAAGGAGTTCACAGATGACATGGGAAACGAGACTTCATATAGCAATCGATGCTGCACAAGGTCTTGAGTACTTGCATCACGGATGCAAGCCGCCTATTGTCCACAGAGATGTGAAGACAGCCAACATTCTCTTAAGCGAAAACTTGGAAGCTAAAATAGCAGATTTCGGTCTTTCCAAGGTTTTTGCTAGTGACATTGAGACTCAAGTGGTCAGCACAGTCATGGGAACAGCCGGATATCTTGATCCAGAATACTACAATTGCCAAAGGCTGAATGAAAAGAGTGATGTGTATAGTTTTGGGGTAGTTCTATTAGAGCTAATCACCGGCCAACCTGCAGTGATAAAAAGTGATGAAGTAATCCACATAGTAACGTGGGTGAGTTCTGAGCTCGAAAAAAGGGAACTAAAAAGTGTTGTGGATCAAAGGATGCAAGAAGAGTTCGATGAGCATTCGGTCTGGAAAGCGTTAGAGATAGCAATGGCGTGCACAACCTCTACCTCTCAACACAGAGTCACAATGGATGTTGTATTGTCTGAGCTCAAAAATTGCTTGGAAATGGAGTTGTCTAGGCATCGAGAAAGAACTCCAACATCAACAGAAGAGCTTCGCGCTGCGTTTGGGCCATATAATCATAGTTCACCTGAAGTTCTCTCCATGTGTACAGATTCCACAAATGTTGATTCCATGACCGGACCGTTTCCTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

891

Amino Acids

98.71

Weight (kDa)

5.34

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 29 - 356 2.2e-110 Malectin-like domain
LRR_8 PF13855 413 - 469 2.2e-09 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 568 - 835 1.6e-49 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 569 - 832 4.5e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 60
Acc36I ACCTGC 1 cut(s) 2305
AccB7I CCANNNNNTGG 1 cut(s) 923
AccI GTMKAC 1 cut(s) 401
AccII CGCG 3 cut(s) 667, 1434, 2574
AccIII TCCGGA 1 cut(s) 756
AciI CCGC 3 cut(s) 536, 1775, 2054
AclWI GGATC 6 cut(s) 683, 696, 999, 1157, 2201, 2391
AcoI YGGCCR 1 cut(s) 2290
AcsI RAATTY 9 cut(s) 194, 310, 368, 527, 563, 890, 999, 1180, 1387
AcuI CTGAAG 3 cut(s) 408, 876, 2628
AcyI GRCGYC 1 cut(s) 387
AfiI CCNNNNNNNGG 7 cut(s) 243, 293, 923, 1094, 1716, 1774, 1828
AflII CTTAAG 1 cut(s) 2095
AjiI CACGTC 1 cut(s) 958
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
Alw21I GWGCWC 4 cut(s) 1839, 2355, 2453, 2505
Alw26I GTCTC 2 cut(s) 1987, 2156
Alw44I GTGCAC 1 cut(s) 2449
AlwI GGATC 6 cut(s) 683, 696, 999, 1157, 2201, 2391
AlwNI CAGNNNCTG 1 cut(s) 1832
Ama87I CYCGRG 2 cut(s) 1304, 1492
Aor13HI TCCGGA 1 cut(s) 756
AoxI GGCC 4 cut(s) 1340, 1866, 2290, 2585
ApaLI GTGCAC 1 cut(s) 2449
ApeKI GCWGC 7 cut(s) 61, 764, 1072, 1277, 1550, 2015, 2576
ApoI RAATTY 9 cut(s) 194, 310, 368, 527, 563, 890, 999, 1180, 1387
Asp700I GAANNNNTTC 1 cut(s) 2568
AspA2I CCTAGG 3 cut(s) 1175, 1811, 2666
AspLEI GCGC 3 cut(s) 61, 66, 2576
AspS9I GGNCC 6 cut(s) 926, 1172, 1340, 1808, 2585, 2657
AsuC2I CCSGG 1 cut(s) 1339
AvaI CYCGRG 2 cut(s) 1304, 1492
AvaII GGWCC 4 cut(s) 926, 1172, 1808, 2657
AvrII CCTAGG 3 cut(s) 1175, 1811, 2666
AxyI CCTNAGG 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 2453
BamHI GGATCC 1 cut(s) 688
BanII GRGCYC 3 cut(s) 1839, 2355, 2505
BarI GAAGNNNNNNTAC 2 cut(s) 1437, 1469
BbsI GAAGAC 2 cut(s) 123, 2084
Bbv12I GWGCWC 4 cut(s) 1839, 2355, 2453, 2505
BbvI GCAGC 7 cut(s) 73, 776, 1084, 1264, 1562, 2002, 2563
BccI CCATC 3 cut(s) 143, 341, 1860
BciVI GTATCC 3 cut(s) 118, 1465, 1874
BcnI CCSGG 1 cut(s) 1339
BcoDI GTCTC 2 cut(s) 1987, 2156
BfaI CTAG 8 cut(s) 1176, 1199, 1556, 1580, 1812, 2151, 2532, 2667
BfmI CTRYAG 4 cut(s) 762, 1210, 1644, 2298
BfoI RGCGCY 1 cut(s) 67
BfrI CTTAAG 1 cut(s) 2095
BfuAI ACCTGC 1 cut(s) 2305
BfuI GTATCC 3 cut(s) 118, 1465, 1874
BisI GCNGC 8 cut(s) 62, 765, 1073, 1278, 1551, 2016, 2054, 2577
BlnI CCTAGG 3 cut(s) 1175, 1811, 2666
BlpI GCTNAGC 1 cut(s) 1833
BlsI GCNGC 8 cut(s) 63, 766, 1074, 1279, 1552, 2017, 2055, 2578
BmcAI AGTACT 3 cut(s) 588, 700, 2033
Bme1390I CCNGG 1 cut(s) 1339
Bme18I GGWCC 4 cut(s) 926, 1172, 1808, 2657
BmeT110I CYCGRG 2 cut(s) 1304, 1492
BmgBI CACGTC 1 cut(s) 958
BmgT120I GGNCC 6 cut(s) 926, 1172, 1340, 1808, 2585, 2657
BmiI GGNNCC 3 cut(s) 690, 1419, 1765
BmrFI CCNGG 1 cut(s) 1339
BmrI ACTGGG 1 cut(s) 18
BmuI ACTGGG 1 cut(s) 18
BoxI GACNNNNGTC 1 cut(s) 480
BpiI GAAGAC 2 cut(s) 123, 2084
BplI GAGNNNNNCTC 2 cut(s) 238, 270
Bpu1102I GCTNAGC 1 cut(s) 1833
BpuEI CTTGAG 5 cut(s) 240, 1267, 1788, 2048, 2151
BpuMI CCSGG 1 cut(s) 1339
Bsa29I ATCGAT 1 cut(s) 2010
BsaBI GATNNNNATC 1 cut(s) 1847
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 9 cut(s) 382, 438, 471, 1175, 1226, 1744, 1811, 2139, 2666
BsaWI WCCGGW 3 cut(s) 145, 756, 2654
BsaXI ACNNNNNCTCC 2 cut(s) 630, 660
Bsc4I CCNNNNNNNGG 7 cut(s) 243, 293, 923, 1094, 1716, 1774, 1828
Bse118I RCCGGY 1 cut(s) 2288
Bse1I ACTGG 6 cut(s) 13, 606, 692, 809, 1343, 1663
Bse21I CCTNAGG 1 cut(s) 242
Bse3DI GCAATG 3 cut(s) 625, 1284, 2448
Bse8I GATNNNNATC 1 cut(s) 1847
BseAI TCCGGA 1 cut(s) 756
BseCI ATCGAT 1 cut(s) 2010
BseDI CCNNGG 9 cut(s) 382, 438, 471, 1175, 1226, 1744, 1811, 2139, 2666
BseGI GGATG 4 cut(s) 154, 2051, 2397, 2491
BseJI GATNNNNATC 1 cut(s) 1847
BseLI CCNNNNNNNGG 7 cut(s) 243, 293, 923, 1094, 1716, 1774, 1828
BseMI GCAATG 3 cut(s) 625, 1284, 2448
BseMII CTCAG 9 cut(s) 256, 788, 842, 1364, 1454, 1824, 1968, 2340, 2490
BseNI ACTGG 6 cut(s) 13, 606, 692, 809, 1343, 1663
BseRI GAGGAG 3 cut(s) 11, 14, 1691
BseSI GKGCMC 1 cut(s) 2453
BseXI GCAGC 7 cut(s) 73, 776, 1084, 1264, 1562, 2002, 2563
BsgI GTGCAG 1 cut(s) 2001
Bsh1236I CGCG 3 cut(s) 667, 1434, 2574
Bsh1285I CGRYCG 1 cut(s) 1716
BshFI GGCC 4 cut(s) 1342, 1868, 2292, 2587
BshVI ATCGAT 1 cut(s) 2010
BsiEI CGRYCG 1 cut(s) 1716
BsiHKAI GWGCWC 4 cut(s) 1839, 2355, 2453, 2505
BsiHKCI CYCGRG 2 cut(s) 1304, 1492
BsiSI CCGG 8 cut(s) 67, 146, 365, 757, 1338, 2196, 2289, 2655
BslFI GGGAC 2 cut(s) 583, 1747
BslI CCNNNNNNNGG 7 cut(s) 243, 293, 923, 1094, 1716, 1774, 1828
BsmAI GTCTC 2 cut(s) 1987, 2156
BsmFI GGGAC 2 cut(s) 583, 1747
BsmI GAATGC 5 cut(s) 784, 829, 1051, 1208, 2413
BsnI GGCC 4 cut(s) 1342, 1868, 2292, 2587
BsoBI CYCGRG 2 cut(s) 1304, 1492
Bsp1286I GDGCHC 4 cut(s) 1839, 2355, 2453, 2505
Bsp13I TCCGGA 1 cut(s) 756
Bsp1407I TGTACA 1 cut(s) 2624
Bsp143I GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
Bsp1720I GCTNAGC 1 cut(s) 1833
Bsp19I CCATGG 1 cut(s) 1744
Bsp68I TCGCGA 1 cut(s) 1434
BspACI CCGC 3 cut(s) 536, 1775, 2054
BspANI GGCC 4 cut(s) 1342, 1868, 2292, 2587
BspCNI CTCAG 9 cut(s) 255, 787, 841, 1363, 1455, 1825, 1967, 2341, 2491
BspDI ATCGAT 1 cut(s) 2010
BspEI TCCGGA 1 cut(s) 756
BspFNI CGCG 3 cut(s) 667, 1434, 2574
BspHI TCATGA 1 cut(s) 1672
BspLI GGNNCC 3 cut(s) 690, 1419, 1765
BspMAI CTGCAG 2 cut(s) 766, 2302
BspMI ACCTGC 1 cut(s) 2305
BspPI GGATC 6 cut(s) 683, 696, 999, 1157, 2201, 2391
BspQI GCTCTTC 2 cut(s) 1434, 2559
BspTI CTTAAG 1 cut(s) 2095
BsrDI GCAATG 3 cut(s) 625, 1284, 2448
BsrFI RCCGGY 1 cut(s) 2288
BsrGI TGTACA 1 cut(s) 2624
BsrI ACTGG 6 cut(s) 13, 606, 692, 809, 1343, 1663
BssAI RCCGGY 1 cut(s) 2288
BssECI CCNNGG 9 cut(s) 382, 438, 471, 1175, 1226, 1744, 1811, 2139, 2666
BssMI GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
BssNI GRCGYC 1 cut(s) 387
BssT1I CCWWGG 6 cut(s) 438, 1175, 1744, 1811, 2139, 2666
Bst4CI ACNGT 6 cut(s) 406, 472, 1738, 1886, 2182, 2661
Bst6I CTCTTC 5 cut(s) 193, 1030, 1434, 2394, 2559
BstACI GRCGYC 1 cut(s) 387
BstAFI CTTAAG 1 cut(s) 2095
BstAPI GCANNNNNTGC 1 cut(s) 2012
BstAUI TGTACA 1 cut(s) 2624
BstC8I GCNNGC 2 cut(s) 2051, 2449
BstDSI CCRYGG 2 cut(s) 471, 1744
BstF5I GGATG 4 cut(s) 154, 2051, 2397, 2491
BstFNI CGCG 3 cut(s) 667, 1434, 2574
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 3 cut(s) 61, 66, 2576
BstKTI GATC 9 cut(s) 376, 655, 691, 994, 1165, 1442, 1716, 2209, 2386
BstMAI GTCTC 2 cut(s) 1987, 2156
BstMBI GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
BstMCI CGRYCG 1 cut(s) 1716
BstMWI GCNNNNNNNGC 5 cut(s) 1212, 1286, 1556, 2012, 2120
BstPAI GACNNNNGTC 1 cut(s) 480
BstSCI CCNGG 1 cut(s) 1337
BstSFI CTRYAG 4 cut(s) 762, 1210, 1644, 2298
BstSLI GKGCMC 1 cut(s) 2453
BstUI CGCG 3 cut(s) 667, 1434, 2574
BstV1I GCAGC 7 cut(s) 73, 776, 1084, 1264, 1562, 2002, 2563
BstV2I GAAGAC 2 cut(s) 123, 2084
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 2010
Bsu36I CCTNAGG 1 cut(s) 242
BsuI GTATCC 3 cut(s) 118, 1465, 1874
BsuRI GGCC 4 cut(s) 1342, 1868, 2292, 2587
BsuTUI ATCGAT 1 cut(s) 2010
BtgI CCRYGG 2 cut(s) 471, 1744
BtrI CACGTC 1 cut(s) 958
BtsCI GGATG 4 cut(s) 154, 2051, 2397, 2491
BtsI GCAGTG 1 cut(s) 2307
BtsIMutI CAGTG 2 cut(s) 1350, 2307
BtuMI TCGCGA 1 cut(s) 1434
BveI ACCTGC 1 cut(s) 2305
Cac8I GCNNGC 2 cut(s) 2051, 2449
CaiI CAGNNNCTG 1 cut(s) 1832
CciI TCATGA 1 cut(s) 1672
CfoI GCGC 3 cut(s) 61, 66, 2576
Cfr10I RCCGGY 1 cut(s) 2288
Cfr13I GGNCC 6 cut(s) 926, 1172, 1340, 1808, 2585, 2657
ClaI ATCGAT 1 cut(s) 2010
CpoI CGGWCCG 1 cut(s) 2657
CseI GACGC 3 cut(s) 376, 656, 1527
CspCI CAANNNNNGTGG 6 cut(s) 453, 488, 1246, 1281, 2623, 2658
CspI CGGWCCG 1 cut(s) 2657
DpnI GATC 9 cut(s) 375, 654, 690, 993, 1164, 1441, 1715, 2208, 2385
DpnII GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
EaeI YGGCCR 1 cut(s) 2290
Eam1104I CTCTTC 5 cut(s) 193, 1030, 1434, 2394, 2559
EarI CTCTTC 5 cut(s) 193, 1030, 1434, 2394, 2559
Ecl136II GAGCTC 3 cut(s) 1837, 2353, 2503
Eco130I CCWWGG 6 cut(s) 438, 1175, 1744, 1811, 2139, 2666
Eco24I GRGCYC 3 cut(s) 1839, 2355, 2505
Eco32I GATATC 3 cut(s) 963, 1690, 2201
Eco47I GGWCC 4 cut(s) 926, 1172, 1808, 2657
Eco53kI GAGCTC 3 cut(s) 1837, 2353, 2503
Eco57I CTGAAG 3 cut(s) 408, 876, 2628
Eco81I CCTNAGG 1 cut(s) 242
Eco88I CYCGRG 2 cut(s) 1304, 1492
EcoICRI GAGCTC 3 cut(s) 1837, 2353, 2503
EcoO109I RGGNCCY 1 cut(s) 1808
EcoRV GATATC 3 cut(s) 963, 1690, 2201
EcoT14I CCWWGG 6 cut(s) 438, 1175, 1744, 1811, 2139, 2666
EcoT38I GRGCYC 3 cut(s) 1839, 2355, 2505
ErhI CCWWGG 6 cut(s) 438, 1175, 1744, 1811, 2139, 2666
FalI AAGNNNNNCTT 2 cut(s) 775, 807
FaqI GGGAC 2 cut(s) 583, 1747
FblI GTMKAC 1 cut(s) 401
Fnu4HI GCNGC 8 cut(s) 62, 765, 1073, 1278, 1551, 2016, 2054, 2577
FokI GGATG 4 cut(s) 161, 2058, 2404, 2498
FriOI GRGCYC 3 cut(s) 1839, 2355, 2505
Fsp4HI GCNGC 8 cut(s) 62, 765, 1073, 1278, 1551, 2016, 2054, 2577
FspBI CTAG 8 cut(s) 1176, 1199, 1556, 1580, 1812, 2151, 2532, 2667
FspI TGCGCA 1 cut(s) 60
GlaI GCGC 3 cut(s) 60, 65, 2575
GluI GCNGC 8 cut(s) 62, 765, 1073, 1278, 1551, 2016, 2054, 2577
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 4 cut(s) 1342, 1868, 2292, 2587
HapII CCGG 8 cut(s) 67, 146, 365, 757, 1338, 2196, 2289, 2655
HgaI GACGC 3 cut(s) 376, 656, 1527
HhaI GCGC 3 cut(s) 61, 66, 2576
Hin1I GRCGYC 1 cut(s) 387
Hin6I GCGC 3 cut(s) 59, 64, 2574
HinP1I GCGC 3 cut(s) 59, 64, 2574
HincII GTYRAC 3 cut(s) 6, 391, 402
HindII GTYRAC 3 cut(s) 6, 391, 402
HpaII CCGG 8 cut(s) 67, 146, 365, 757, 1338, 2196, 2289, 2655
Hpy99I CGWCG 3 cut(s) 406, 712, 1543
HpyAV CCTTC 7 cut(s) 235, 337, 690, 1040, 1187, 1387, 1639
HpyCH4III ACNGT 6 cut(s) 406, 472, 1738, 1886, 2182, 2661
HpyCH4IV ACGT 4 cut(s) 278, 957, 1122, 2336
HpyF10VI GCNNNNNNNGC 5 cut(s) 1212, 1286, 1556, 2012, 2120
HpySE526I ACGT 4 cut(s) 278, 957, 1122, 2336
Hsp92I GRCGYC 1 cut(s) 387
HspAI GCGC 3 cut(s) 59, 64, 2574
Kpn2I TCCGGA 1 cut(s) 756
Kzo9I GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
LguI GCTCTTC 2 cut(s) 1434, 2559
LmnI GCTCC 2 cut(s) 1531, 1842
Lsp1109I GCAGC 7 cut(s) 73, 776, 1084, 1264, 1562, 2002, 2563
MaeI CTAG 8 cut(s) 1176, 1199, 1556, 1580, 1812, 2151, 2532, 2667
MaeII ACGT 4 cut(s) 278, 957, 1122, 2336
MaeIII GTNAC 6 cut(s) 433, 668, 941, 2153, 2332, 2476
MalI GATC 9 cut(s) 375, 654, 690, 993, 1164, 1441, 1715, 2208, 2385
MboI GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
MfeI CAATTG 2 cut(s) 1403, 2221
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 4 cut(s) 1839, 2355, 2453, 2505
MlyI GAGTC 4 cut(s) 1016, 1316, 2158, 2484
MmeI TCCRAC 3 cut(s) 261, 504, 2576
MroI TCCGGA 1 cut(s) 756
MroXI GAANNNNTTC 1 cut(s) 2568
MseI TTAA 2 cut(s) 540, 2096
MslI CAYNNNNRTG 2 cut(s) 2070, 2183
MspA1I CMGCKG 1 cut(s) 1075
MspCI CTTAAG 1 cut(s) 2095
MspI CCGG 8 cut(s) 67, 146, 365, 757, 1338, 2196, 2289, 2655
MspR9I CCNGG 1 cut(s) 1339
MteI GCGCNGCGC 1 cut(s) 62
MunI CAATTG 2 cut(s) 1403, 2221
Mva1269I GAATGC 5 cut(s) 784, 829, 1051, 1208, 2413
MvnI CGCG 3 cut(s) 667, 1434, 2574
MwoI GCNNNNNNNGC 5 cut(s) 1212, 1286, 1556, 2012, 2120
NciI CCSGG 1 cut(s) 1339
NcoI CCATGG 1 cut(s) 1744
NdeII GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
NlaIV GGNNCC 3 cut(s) 690, 1419, 1765
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 3 cut(s) 668, 2153, 2476
NruI TCGCGA 1 cut(s) 1434
NsbI TGCGCA 1 cut(s) 60
PaeR7I CTCGAG 1 cut(s) 1304
PagI TCATGA 1 cut(s) 1672
PciSI GCTCTTC 2 cut(s) 1434, 2559
PcsI WCGNNNNNNNCGW 1 cut(s) 1431
PctI GAATGC 5 cut(s) 784, 829, 1051, 1208, 2413
PdmI GAANNNNTTC 1 cut(s) 2568
PfeI GAWTC 9 cut(s) 211, 461, 877, 1039, 1082, 1607, 1848, 2630, 2645
PflMI CCANNNNNTGG 1 cut(s) 923
PkrI GCNGC 8 cut(s) 63, 766, 1074, 1279, 1552, 2017, 2055, 2578
Ple19I CGATCG 1 cut(s) 1716
PleI GAGTC 4 cut(s) 1016, 1315, 2158, 2483
PpsI GAGTC 4 cut(s) 1016, 1315, 2158, 2483
PpuMI RGGWCCY 1 cut(s) 1808
PshAI GACNNNNGTC 1 cut(s) 480
Psp124BI GAGCTC 3 cut(s) 1839, 2355, 2505
Psp5II RGGWCCY 1 cut(s) 1808
PspN4I GGNNCC 3 cut(s) 690, 1419, 1765
PspPI GGNCC 6 cut(s) 926, 1172, 1340, 1808, 2585, 2657
PspPPI RGGWCCY 1 cut(s) 1808
PspXI VCTCGAGB 1 cut(s) 1304
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
PstI CTGCAG 2 cut(s) 766, 2302
PstNI CAGNNNCTG 1 cut(s) 1832
PsuI RGATCY 1 cut(s) 688
PvuI CGATCG 1 cut(s) 1716
PvuII CAGCTG 1 cut(s) 1075
RruI TCGCGA 1 cut(s) 1434
RseI CAYNNNNRTG 2 cut(s) 2070, 2183
Rsr2I CGGWCCG 1 cut(s) 2657
RsrII CGGWCCG 1 cut(s) 2657
SacI GAGCTC 3 cut(s) 1839, 2355, 2505
SalI GTCGAC 1 cut(s) 400
SapI GCTCTTC 2 cut(s) 1434, 2559
SaqAI TTAA 2 cut(s) 540, 2096
SatI GCNGC 8 cut(s) 62, 765, 1073, 1278, 1551, 2016, 2054, 2577
Sau3AI GATC 9 cut(s) 373, 652, 688, 991, 1162, 1439, 1713, 2206, 2383
Sau96I GGNCC 6 cut(s) 926, 1172, 1340, 1808, 2585, 2657
ScaI AGTACT 3 cut(s) 588, 700, 2033
SchI GAGTC 4 cut(s) 1016, 1316, 2158, 2484
ScrFI CCNGG 1 cut(s) 1339
SduI GDGCHC 4 cut(s) 1839, 2355, 2453, 2505
SfcI CTRYAG 4 cut(s) 762, 1210, 1644, 2298
Sfr274I CTCGAG 1 cut(s) 1304
SinI GGWCC 4 cut(s) 926, 1172, 1808, 2657
SlaI CTCGAG 1 cut(s) 1304
SmiMI CAYNNNNRTG 2 cut(s) 2070, 2183
SmlI CTYRAG 7 cut(s) 255, 1246, 1304, 1803, 2027, 2095, 2166
SmoI CTYRAG 7 cut(s) 255, 1246, 1304, 1803, 2027, 2095, 2166
SsiI CCGC 3 cut(s) 536, 1775, 2054
SspMI CTAG 8 cut(s) 1176, 1199, 1556, 1580, 1812, 2151, 2532, 2667
SstI GAGCTC 3 cut(s) 1839, 2355, 2505
StyD4I CCNGG 1 cut(s) 1337
StyI CCWWGG 6 cut(s) 438, 1175, 1744, 1811, 2139, 2666
TaaI ACNGT 6 cut(s) 406, 472, 1738, 1886, 2182, 2661
TaiI ACGT 4 cut(s) 281, 960, 1125, 2339
TaqII GACCGA 2 cut(s) 2120, 2407
TatI WGTACW 8 cut(s) 586, 698, 801, 846, 1459, 1920, 2031, 2624
TauI GCSGC 1 cut(s) 2056
TfiI GAWTC 9 cut(s) 211, 461, 877, 1039, 1082, 1607, 1848, 2630, 2645
Tru1I TTAA 2 cut(s) 540, 2096
Tru9I TTAA 2 cut(s) 540, 2096
TscAI CASTG 2 cut(s) 1350, 2307
TseFI GTSAC 3 cut(s) 668, 2153, 2476
TseI GCWGC 7 cut(s) 61, 764, 1072, 1277, 1550, 2015, 2576
Tsp45I GTSAC 3 cut(s) 668, 2153, 2476
TspDTI ATGAA 8 cut(s) 67, 502, 836, 1488, 1769, 1988, 2253, 2331
TspGWI ACGGA 4 cut(s) 137, 744, 1776, 2058
TspRI CASTG 2 cut(s) 1350, 2307
Van91I CCANNNNNTGG 1 cut(s) 923
Vha464I CTTAAG 1 cut(s) 2095
VneI GTGCAC 1 cut(s) 2449
VpaK11BI GGWCC 4 cut(s) 926, 1172, 1808, 2657
XapI RAATTY 9 cut(s) 194, 310, 368, 527, 563, 890, 999, 1180, 1387
XhoI CTCGAG 1 cut(s) 1304
XmaJI CCTAGG 3 cut(s) 1175, 1811, 2666
XmiI GTMKAC 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 2568
XspI CTAG 8 cut(s) 1176, 1199, 1556, 1580, 1812, 2151, 2532, 2667
ZrmI AGTACT 3 cut(s) 588, 700, 2033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.