RchiOBHm_Chr2g0092431

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005945
Physical Location & Seq
Reverse (-)
5954016 .. 5956100
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46754

Sequence Viewer

Length: 1518 bp
ATGTCAACTGCTGCCTTCCTCCTCCTGTATCTGATGATCTCTGCAAGTTCAGTTCACGCGCAGCGCCGAATAGCCTTCATAAGCATTGATTGTGGAGCTCCCTCAAAGCTGTATGAAGACCCGGATACCAACATAACTTACTCACCAGATGACAATTTCATAGACACAGGGATAAGCCAGAACATCTCATCCGAATATATGTATCCTAACAATCCAAACTTGCCATTACCTGCCTCGGACCTTAGGAGTTTTCCTCATGGAAACAAAAACTGCTACACTTTAAAACCTGAAGCTGGGAAAGGTAGTTTGAACCTAATCCGAGCTTCTTTCTTGTATGGAAACTACGATGGAAAAGACCAGCTACCGGAGTTTGATCTCTATCTTGATGTCAACTTCTGGTCTGTGGTGACATTTGGAAATGCTTCTGATATTGTCATAAAGGAGATTTTTGGTTTTGCACAATCTGATGTCATAAATGTTTGTCTTCTAAACAAAGGTTTAGGAACTCCTTTCATTTCGGCATTGGAACTTAGACCGCTTAACAGCTCAAATTATGGGACTGCGTTTGGGACTAATGCATCATTAGTACTCTTTCAAAGACTAGACATTGGATCAAGCAATGGAACAGGTAGGTATGCAGAAGATGTTTATGACCGCGTTTGGTCTAATTACATCTCTCCTTATTGGGATTCAGTGAGCACTTCTTCACCAATTACTACTGATGAAGATGGATATAGAGTACCTTATGAAGTAATCAAGACTGCAGCTAGGCCTCGGAATGGAAGTGAACCTCTGGAGCTGTATTGGAACACAACTGACTCGAATGCTCAGTTTTATGTTTATATGTACTTTTCTGAAGTGGAGCATCTTGAGAAAAACCAGTCAAGAAAGTTGAATATATCTTGGAATGGTGCTCCATTGTTTGAACAATTTGTGCCTCGTTACTTATATGCAGACATATTATCTAATTCAAAATCTTTGGTGGGAAAAGAGCACCAGATTTCTATATCTAAAGCAGAAAACTCGACCCTTCCACCGATTCTCAATGCAGTTGAGATTTACCAAGTCATGCAGCTGGTTGAATCACCAACTTCTAGTGAAGATGTTGATGCTATTATGAATGTCAAAACCACATATCAAATTAAAAAAACCTGGGCTGGTGATCCCTGTGCACCTAAGAATTTCTCTTGGGAAGGTCTTAAATGCAACTACACCATTTCAGTTCCTCGGATTATTTCCCTGAACTTGACCTCAAGCAATTTGAGTGGGATTATAGCTGCTTCCATTTCCAAGCTTTCATCACTGGAGTCCTTGGACTTGTCAAACAATAACTTAACCGGACCAGTGCCTGCATTTCTGGAAGAACTAAAATCTCTCAAGACTCTGAATTTGAAAGGGAATCAATTGTCTGGTTTTGTTCCAAATGCACTTAAGAAAAGATCAGATGCTGGATTACTTGTACTAAGGTACACCTTCTCATCTCAAACTGAGAAAACTTTCTTCTTGTTTATGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

505

Amino Acids

56.24

Weight (kDa)

5.12

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 29 - 356 1.5e-108 Malectin-like domain
LRR_8 PF13855 413 - 469 2.4e-09 Leucine rich repeat
LRR_4 PF12799 434 - 470 4.8e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AccII CGCG 2 cut(s) 59, 657
AciI CCGC 2 cut(s) 536, 655
AclWI GGATC 2 cut(s) 619, 1157
AcsI RAATTY 2 cut(s) 1180, 1387
AcuI CTGAAG 2 cut(s) 309, 876
AfaI GTAC 5 cut(s) 588, 741, 848, 1461, 1469
AfiI CCNNNNNNNGG 3 cut(s) 293, 364, 779
AflII CTTAAG 1 cut(s) 1430
AgsI TTSAA 7 cut(s) 310, 596, 895, 926, 972, 1082, 1393
AjnI CCWGG 1 cut(s) 1151
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
Alw21I GWGCWC 5 cut(s) 100, 701, 916, 996, 1174
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 2 cut(s) 619, 1157
AlwNI CAGNNNCTG 2 cut(s) 1349, 1448
AoxI GGCC 1 cut(s) 770
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 5 cut(s) 11, 61, 764, 1072, 1277
ApoI RAATTY 2 cut(s) 1180, 1387
ArsI GACNNNNNNTTYG 2 cut(s) 1372, 1404
Asp700I GAANNNNTTC 2 cut(s) 421, 1496
AspLEI GCGC 2 cut(s) 61, 66
AspS9I GGNCC 2 cut(s) 238, 1340
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 5 cut(s) 135, 418, 699, 1077, 1172
AvaII GGWCC 2 cut(s) 238, 1340
AxyI CCTNAGG 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 1174
BanII GRGCYC 1 cut(s) 100
BbsI GAAGAC 2 cut(s) 123, 476
Bbv12I GWGCWC 5 cut(s) 100, 701, 916, 996, 1174
BbvI GCAGC 4 cut(s) 73, 776, 1084, 1264
BccI CCATC 2 cut(s) 341, 722
BcgI CGANNNNNNTGC 2 cut(s) 1005, 1039
BciT130I CCWGG 1 cut(s) 1153
BciVI GTATCC 2 cut(s) 118, 213
BcnI CCSGG 1 cut(s) 122
BfaI CTAG 3 cut(s) 602, 768, 1095
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 1 cut(s) 67
BfrI CTTAAG 1 cut(s) 1430
BfuAI ACCTGC 1 cut(s) 238
BfuI GTATCC 2 cut(s) 118, 213
BisI GCNGC 5 cut(s) 12, 62, 765, 1073, 1278
BlsI GCNGC 5 cut(s) 13, 63, 766, 1074, 1279
BmcAI AGTACT 1 cut(s) 588
Bme1390I CCNGG 2 cut(s) 122, 1153
Bme18I GGWCC 2 cut(s) 238, 1340
BmgT120I GGNCC 2 cut(s) 238, 1340
BmrFI CCNGG 2 cut(s) 122, 1153
BmsI GCATC 4 cut(s) 587, 874, 1099, 1435
BpiI GAAGAC 2 cut(s) 123, 476
BplI GAGNNNNNCTC 2 cut(s) 238, 270
BpmI CTGGAG 2 cut(s) 815, 1325
BpuEI CTTGAG 3 cut(s) 890, 1237, 1361
BpuMI CCSGG 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 5 cut(s) 234, 773, 1152, 1226, 1311
BsaWI WCCGGW 2 cut(s) 364, 1337
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 364, 779
Bse1I ACTGG 3 cut(s) 880, 1308, 1343
Bse21I CCTNAGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 625
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 1 cut(s) 1153
BseDI CCNNGG 5 cut(s) 234, 773, 1152, 1226, 1311
BseGI GGATG 1 cut(s) 188
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 3 cut(s) 293, 364, 779
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 2 cut(s) 842, 1479
BseNI ACTGG 3 cut(s) 880, 1308, 1343
BseRI GAGGAG 1 cut(s) 11
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 4 cut(s) 73, 776, 1084, 1264
BseYI CCCAGC 1 cut(s) 293
Bsh1236I CGCG 2 cut(s) 59, 657
BshFI GGCC 1 cut(s) 772
BsiHKAI GWGCWC 5 cut(s) 100, 701, 916, 996, 1174
BsiSI CCGG 3 cut(s) 122, 365, 1338
BslFI GGGAC 2 cut(s) 571, 583
BslI CCNNNNNNNGG 3 cut(s) 293, 364, 779
BsmFI GGGAC 2 cut(s) 571, 583
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 1 cut(s) 772
Bsp1286I GDGCHC 5 cut(s) 100, 701, 916, 996, 1174
Bsp143I GATC 5 cut(s) 36, 373, 611, 1162, 1439
BspACI CCGC 2 cut(s) 536, 655
BspANI GGCC 1 cut(s) 772
BspCNI CTCAG 2 cut(s) 841, 1480
BspFNI CGCG 2 cut(s) 59, 657
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 2 cut(s) 619, 1157
BspTI CTTAAG 1 cut(s) 1430
BsrDI GCAATG 1 cut(s) 625
BsrI ACTGG 3 cut(s) 880, 1308, 1343
BssECI CCNNGG 5 cut(s) 234, 773, 1152, 1226, 1311
BssMI GATC 5 cut(s) 36, 373, 611, 1162, 1439
BssT1I CCWWGG 1 cut(s) 1311
Bst2UI CCWGG 1 cut(s) 1153
BstAFI CTTAAG 1 cut(s) 1430
BstC8I GCNNGC 1 cut(s) 1350
BstDEI CTNAG 6 cut(s) 242, 530, 828, 1176, 1463, 1488
BstF5I GGATG 1 cut(s) 188
BstFNI CGCG 2 cut(s) 59, 657
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 2 cut(s) 61, 66
BstKTI GATC 5 cut(s) 39, 376, 614, 1165, 1442
BstMBI GATC 5 cut(s) 36, 373, 611, 1162, 1439
BstNI CCWGG 1 cut(s) 1153
BstSCI CCNGG 2 cut(s) 120, 1151
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 1174
BstUI CGCG 2 cut(s) 59, 657
BstV1I GCAGC 4 cut(s) 73, 776, 1084, 1264
BstV2I GAAGAC 2 cut(s) 123, 476
Bsu36I CCTNAGG 1 cut(s) 242
BsuI GTATCC 2 cut(s) 118, 213
BsuRI GGCC 1 cut(s) 772
BtsCI GGATG 1 cut(s) 188
BtsIMutI CAGTG 3 cut(s) 699, 1301, 1350
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 1350
CaiI CAGNNNCTG 2 cut(s) 1349, 1448
CfoI GCGC 2 cut(s) 61, 66
Cfr13I GGNCC 2 cut(s) 238, 1340
Csp6I GTAC 5 cut(s) 587, 740, 847, 1460, 1468
CspCI CAANNNNNGTGG 2 cut(s) 1246, 1281
CviAII CATG 2 cut(s) 257, 1069
CviQI GTAC 5 cut(s) 587, 740, 847, 1460, 1468
DdeI CTNAG 6 cut(s) 242, 530, 828, 1176, 1463, 1488
DpnI GATC 5 cut(s) 38, 375, 613, 1164, 1441
DpnII GATC 5 cut(s) 36, 373, 611, 1162, 1439
DraI TTTAAA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 1311
Eco147I AGGCCT 1 cut(s) 772
Eco24I GRGCYC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 238, 1340
Eco53kI GAGCTC 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 309, 876
Eco81I CCTNAGG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 1151
EcoT14I CCWWGG 1 cut(s) 1311
EcoT22I ATGCAT 1 cut(s) 580
EcoT38I GRGCYC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 1311
FaeI CATG 2 cut(s) 260, 1072
FaqI GGGAC 2 cut(s) 571, 583
FatI CATG 2 cut(s) 256, 1068
Fnu4HI GCNGC 5 cut(s) 12, 62, 765, 1073, 1278
FokI GGATG 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 5 cut(s) 12, 62, 765, 1073, 1278
FspBI CTAG 3 cut(s) 602, 768, 1095
GlaI GCGC 2 cut(s) 60, 65
GluI GCNGC 5 cut(s) 12, 62, 765, 1073, 1278
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 2 cut(s) 815, 1325
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 1 cut(s) 772
HapII CCGG 3 cut(s) 122, 365, 1338
HhaI GCGC 2 cut(s) 61, 66
Hin1II CATG 2 cut(s) 260, 1072
Hin6I GCGC 2 cut(s) 59, 64
HinP1I GCGC 2 cut(s) 59, 64
HincII GTYRAC 2 cut(s) 6, 391
HindII GTYRAC 2 cut(s) 6, 391
HindIII AAGCTT 1 cut(s) 1292
HinfI GANTC 7 cut(s) 689, 818, 1039, 1082, 1307, 1381, 1399
HpaII CCGG 3 cut(s) 122, 365, 1338
HphI GGTGA 5 cut(s) 135, 418, 699, 1077, 1172
Hpy166II GTNNAC 6 cut(s) 6, 55, 391, 788, 1172, 1470
Hpy188III TCNNGA 7 cut(s) 383, 757, 794, 869, 885, 1358, 1378
Hpy8I GTNNAC 6 cut(s) 6, 55, 391, 788, 1172, 1470
HpyAV CCTTC 5 cut(s) 25, 85, 1040, 1187, 1483
HpyF3I CTNAG 6 cut(s) 242, 530, 828, 1176, 1463, 1488
Hsp92II CATG 2 cut(s) 260, 1072
HspAI GCGC 2 cut(s) 59, 64
Kzo9I GATC 5 cut(s) 36, 373, 611, 1162, 1439
LmnI GCTCC 5 cut(s) 95, 103, 796, 862, 919
Lsp1109I GCAGC 4 cut(s) 73, 776, 1084, 1264
LweI GCATC 4 cut(s) 587, 874, 1099, 1435
MaeI CTAG 3 cut(s) 602, 768, 1095
MaeIII GTNAC 2 cut(s) 406, 941
MalI GATC 5 cut(s) 38, 375, 613, 1164, 1441
MboI GATC 5 cut(s) 36, 373, 611, 1162, 1439
MboII GAAGA 8 cut(s) 128, 476, 653, 696, 737, 1112, 1373, 1492
MfeI CAATTG 1 cut(s) 1403
MhlI GDGCHC 5 cut(s) 100, 701, 916, 996, 1174
MlyI GAGTC 3 cut(s) 812, 1316, 1375
Mph1103I ATGCAT 1 cut(s) 580
MroXI GAANNNNTTC 2 cut(s) 421, 1496
MseI TTAA 6 cut(s) 281, 540, 1143, 1200, 1334, 1431
MspA1I CMGCKG 1 cut(s) 1075
MspCI CTTAAG 1 cut(s) 1430
MspI CCGG 3 cut(s) 122, 365, 1338
MspR9I CCNGG 2 cut(s) 122, 1153
MteI GCGCNGCGC 1 cut(s) 62
MunI CAATTG 1 cut(s) 1403
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 1 cut(s) 1153
MvnI CGCG 2 cut(s) 59, 657
NciI CCSGG 1 cut(s) 122
NdeII GATC 5 cut(s) 36, 373, 611, 1162, 1439
NlaIII CATG 2 cut(s) 260, 1072
NmuCI GTSAC 1 cut(s) 406
NsiI ATGCAT 1 cut(s) 580
PceI AGGCCT 1 cut(s) 772
PctI GAATGC 1 cut(s) 829
PdmI GAANNNNTTC 2 cut(s) 421, 1496
PfeI GAWTC 4 cut(s) 689, 1039, 1082, 1399
PkrI GCNGC 5 cut(s) 13, 63, 766, 1074, 1279
PleI GAGTC 3 cut(s) 812, 1315, 1375
PpsI GAGTC 3 cut(s) 812, 1315, 1375
Psp124BI GAGCTC 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 1151
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 1151
PspPI GGNCC 2 cut(s) 238, 1340
PstI CTGCAG 1 cut(s) 766
PstNI CAGNNNCTG 2 cut(s) 1349, 1448
PvuII CAGCTG 1 cut(s) 1075
RsaI GTAC 5 cut(s) 588, 741, 848, 1461, 1469
RsaNI GTAC 5 cut(s) 587, 740, 847, 1460, 1468
SacI GAGCTC 1 cut(s) 100
SaqAI TTAA 6 cut(s) 281, 540, 1143, 1200, 1334, 1431
SatI GCNGC 5 cut(s) 12, 62, 765, 1073, 1278
Sau3AI GATC 5 cut(s) 36, 373, 611, 1162, 1439
Sau96I GGNCC 2 cut(s) 238, 1340
ScaI AGTACT 1 cut(s) 588
SchI GAGTC 3 cut(s) 812, 1316, 1375
ScrFI CCNGG 2 cut(s) 122, 1153
SduI GDGCHC 5 cut(s) 100, 701, 916, 996, 1174
SfaNI GCATC 4 cut(s) 587, 874, 1099, 1435
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 2 cut(s) 238, 1340
SmlI CTYRAG 4 cut(s) 869, 1252, 1376, 1430
SmoI CTYRAG 4 cut(s) 869, 1252, 1376, 1430
SseBI AGGCCT 1 cut(s) 772
SsiI CCGC 2 cut(s) 536, 655
SspMI CTAG 3 cut(s) 602, 768, 1095
SstI GAGCTC 1 cut(s) 100
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 2 cut(s) 120, 1151
StyI CCWWGG 1 cut(s) 1311
TaqI TCGA 2 cut(s) 821, 1025
TatI WGTACW 3 cut(s) 586, 846, 1459
TfiI GAWTC 4 cut(s) 689, 1039, 1082, 1399
Tru1I TTAA 6 cut(s) 281, 540, 1143, 1200, 1334, 1431
Tru9I TTAA 6 cut(s) 281, 540, 1143, 1200, 1334, 1431
TscAI CASTG 3 cut(s) 699, 1308, 1350
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 5 cut(s) 11, 61, 764, 1072, 1277
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 8 cut(s) 67, 129, 148, 502, 738, 762, 1133, 1287
TspRI CASTG 3 cut(s) 699, 1308, 1350
Vha464I CTTAAG 1 cut(s) 1430
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 2 cut(s) 238, 1340
XapI RAATTY 2 cut(s) 1180, 1387
XmnI GAANNNNTTC 2 cut(s) 421, 1496
XspI CTAG 3 cut(s) 602, 768, 1095
ZrmI AGTACT 1 cut(s) 588
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.