Rroxscaffold_2G00149130

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005945
Physical Location & Seq
Forward (+)
86722915 .. 86727557
4643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149130.1

Sequence Viewer

Length: 2688 bp
ATGGACAGAGAAATGAAAATGTCAACTGCTGCCTTCCTCCTCCTCTTTCTGATGATCTCTGCAAGTTCAGTTCACGCGCAGCGCCGAATAGCCTTCATAAGCATTGATTGTGGAGCTCCCTCAAAGCTGTATGAAGACCCGGATACCAACATAACTTACTCACCAGATGACAATTTCATCGACACAGGGATAAGCCAGAACATCTCATCCGACTATATGTATCCTAACAATCCAAACTTGCCATTACCTGCCTCAGACCTTAGGAGTTTTCCTCATGGAAACAAAAACTGCTACACTTTAAAACCTGAAGCTGGGAAAGGTAGTTTGAACCTAATCCGAGCTTCTTTCTTGTATGGAAACTACGATGGAAAAGACCAGCTACCAGAGTTTGATCTCTATCTTGATGTCAACTTCTGGTCTGTGGTGACATTTGGAAATGCTTCTGATATTGTCATAAAGGAGATTGTTGGTTTTGCACAATCTGATGTCATAAATGTTTGTCTTCTAAACAAAGGTTTAGGAACTCCTTTCATTTCGGCATTGGAACTTAGACCGCTTAACAGCTCAAATTATGGGACTGCGTTTGGGACTAATGCATCATTAGTACTCTTTCAAAGACTAGACATTGGATCAAGCAATGGAACAGGTAGGTATGCAGAGGATGTTTATGACCGCGTTTGGTCTAATTACATCTCTCCTTCTTGGGATTCAGTGAGCACTTCTTCACCAATTACTACTGATGAAGATGGATATAGAGTACCTTATGAAGTAATCAAGACTGCAGCTAGGCCTCGGAATGGAAGTGAACCTCTGGAGCTGTATTGGAACACAACTGACTCGAATGCTCAGTTTTATGTTTATATGTACTTTTCTGAAGTGGAGCATCTTGAGAAAAACCAGTCAAGAAAGTTGAACATATCTTGGAATGGTGCTCTATTGTTTGGACAATTTGTGCCTCGTTACTTATATGCAGACATCTTATCTAATTCAAAATCTTTGGTGGGAAAAGATCACCAGATTTCTATATCTAAAGCAGAAAACTCGACCCTTCCACCAATTCTCAATGCGGTTGAGATTTACCAAGTCATGCAGCTGGTTGAATCACCAACATCTAGTGAAGATGTTGATGCCATTATGAATGTCAAAACCACATATCAAATTAAAAAAACCTGGGCTGGTGATCCCTGTGCACCTAAGAATTTCTCTTGGGAAGGTCTTAAGTGCAACTACAGCATTTCAGTTCCTCGGATTATTTCCCTGAACTTGACCTCAAGCAATTTGAGTGGGATTATAGCTGCTTCCATTTCCAAGCTTTCATCATTGGAGTCCTTGGACTTGTCAAACAATAACTTAACCGGACCAGTGCCTGCATTTCTGGAAGAACTAAAATCTCTCAAGACTCTGAATTTGAAAGGGAATCAATTGTCTGGTTTTGTTCCAAATGCACTTAAGAAAAGATCAGATGCTGGATTACTTGTACTAAGTGTGGATAGTCAAAATGTTTGTGGCTCAAATTCATGCAAAAAAAAGAAGAAAATTGTTGCTCCAATAGTTGGATCATTATTATCAGCACTAGTAGTCCTTATAGTTCTCATACTTGTCTGGAAGCTGAGAACAAAAAAGAACTCAGAAGGAGTAGAAACCTTTAAGAAGGAAGGAATTCCTATTGCATCAAAGAAGTGCCAGTTTAGTTATGATGAGGTTATAGAAATCACCAAAAATTTCAACACTGAAATCGGAAAAGGAGGATTCGGGGCTGTGTACCATGGCTACATGAAAGATGGCACCCAAGTTGCAGTCAAAATGCTTTCTCCATCATCAACTCAAGGACCGAGGGAGTTCCAAACTGAGGCTGAGCTCCTGATGAGAATCCATCACAGAAATTTGGCATCATTTATTGGATACTGTGATGATGCTGATAACCTGGCTCTTATATATGAGTACATGGCTAATGGGAACCTTAAAGATTATCTCTCAGTTAAAAGCTCAGGTATGACTTGGGAATTGAGACTTCGCATCGCCATAGATGCTGCACAAGGGCTAGAGTACTTACACCATGGATGCAATCCACCTATTGTACACAGAGATGTGAAGACTGCCAACATTCTCTTAAGTGAAAACTTGGAAGCTAAGATAGCAGATTTTGGTCTTTCTAAGGTTTTTTCCAATGATAATGAAACCGACGTGGAAACCACAGTTATGGGGACAGCAGGTTATTTAGACCCAGAGTACTACAGTTCTCACAAGTTGAATGAAAAAAGCGACGTGTATAGTTTCGGGGTAGTACTATTGGAACTGATCACAGGCCAACCTGCAGTCATAAGAAGCGATGAACGAATTCACATTGTGCAGTGGGTGAGTCCTGAACTCCAGAAAGGGGATATAGCAAGTGTTGTTGATCAAAGAATCGAAGGAGATTTCGATGTGAATTCAGTTTGGAAAGCTTTCGAAATAGCAATGACATGCACAACTAGAACCTCTCAACAAAGAGCCACCATGGGCATTGTGTTATCAGAGCTAAAACAGTGTTTGGAAATGGAGTTGTCGAGGAATCGAGAAAGAAAGTCTGGATTGACTGAAGAAAGTAGAGATAGATTTGCAGTAGCATATAGTAGTTCACCATATAATAGTTCCATGTATGCAGATTCCACAAATGCTGATTCCATGACCGGGCCATTTGCTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

895

Amino Acids

99.24

Weight (kDa)

5.44

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 35 - 362 5.5e-108 Malectin-like domain
LRR_8 PF13855 419 - 475 8.2e-10 Leucine rich repeat
LRR_4 PF12799 440 - 476 9e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 575 - 839 6e-51 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 576 - 832 2.6e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 256, 2201, 2320
AccB1I GGYRCC 1 cut(s) 1784
AccB7I CCANNNNNTGG 1 cut(s) 1553
AccII CGCG 2 cut(s) 77, 675
AciI CCGC 3 cut(s) 554, 673, 1067
AclWI GGATC 3 cut(s) 637, 1175, 1564
AcsI RAATTY 8 cut(s) 1198, 1405, 1513, 1659, 1720, 1882, 2337, 2428
AcuI CTGAAG 3 cut(s) 327, 894, 2598
AdeI CACNNNGTG 1 cut(s) 2347
AfiI CCNNNNNNNGG 6 cut(s) 311, 797, 1553, 1849, 2377, 2670
AflII CTTAAG 3 cut(s) 1217, 1448, 2110
AflIII ACRYGT 1 cut(s) 2265
AgsI TTSAA 8 cut(s) 328, 614, 913, 990, 1100, 1411, 1726, 2251
AhlI ACTAGT 1 cut(s) 1573
AjiI CACGTC 2 cut(s) 2185, 2266
AjnI CCWGG 2 cut(s) 1169, 1923
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
Alw21I GWGCWC 5 cut(s) 118, 719, 934, 1192, 1860
Alw26I GTCTC 1 cut(s) 2002
Alw44I GTGCAC 1 cut(s) 1188
AlwI GGATC 3 cut(s) 637, 1175, 1564
AlwNI CAGNNNCTG 2 cut(s) 1367, 1466
AoxI GGCC 3 cut(s) 788, 2305, 2672
ApaLI GTGCAC 1 cut(s) 1188
ApeKI GCWGC 6 cut(s) 29, 79, 782, 1090, 1295, 2030
ApoI RAATTY 8 cut(s) 1198, 1405, 1513, 1659, 1720, 1882, 2337, 2428
ArsI GACNNNNNNTTYG 2 cut(s) 1390, 1422
Asp700I GAANNNNTTC 4 cut(s) 439, 1659, 2337, 2444
AspLEI GCGC 2 cut(s) 79, 84
AspS9I GGNCC 3 cut(s) 1358, 1829, 2672
AsuC2I CCSGG 2 cut(s) 140, 2671
AsuHPI GGTGA 9 cut(s) 153, 436, 717, 1004, 1095, 1190, 1705, 2368, 2610
AsuII TTCGAA 1 cut(s) 2448
AvaII GGWCC 2 cut(s) 1358, 1829
AxyI CCTNAGG 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 1192
BanI GGYRCC 1 cut(s) 1784
BanII GRGCYC 2 cut(s) 118, 1860
BbsI GAAGAC 3 cut(s) 141, 494, 2099
Bbv12I GWGCWC 5 cut(s) 118, 719, 934, 1192, 1860
BbvI GCAGC 6 cut(s) 16, 91, 794, 1102, 1282, 2017
BccI CCATC 5 cut(s) 359, 740, 1775, 1822, 1881
BcgI CGANNNNNNTGC 2 cut(s) 1023, 1057
BciT130I CCWGG 2 cut(s) 1171, 1925
BciVI GTATCC 3 cut(s) 136, 231, 1895
BclI TGATCA 2 cut(s) 2298, 2398
BcnI CCSGG 2 cut(s) 140, 2671
BcoDI GTCTC 1 cut(s) 2002
BcuI ACTAGT 1 cut(s) 1573
BfaI CTAG 7 cut(s) 620, 786, 1113, 1574, 2042, 2472, 2682
BfmI CTRYAG 4 cut(s) 780, 1228, 2233, 2313
BfoI RGCGCY 1 cut(s) 85
BfrI CTTAAG 3 cut(s) 1217, 1448, 2110
BfuAI ACCTGC 3 cut(s) 256, 2201, 2320
BfuI GTATCC 3 cut(s) 136, 231, 1895
BisI GCNGC 6 cut(s) 30, 80, 783, 1091, 1296, 2031
BlpI GCTNAGC 1 cut(s) 1854
BlsI GCNGC 6 cut(s) 31, 81, 784, 1092, 1297, 2032
BmcAI AGTACT 4 cut(s) 606, 2048, 2231, 2286
Bme1390I CCNGG 4 cut(s) 140, 1171, 1925, 2671
Bme18I GGWCC 2 cut(s) 1358, 1829
BmgBI CACGTC 2 cut(s) 2185, 2266
BmgT120I GGNCC 3 cut(s) 1358, 1829, 2672
BmiI GGNNCC 2 cut(s) 1786, 1958
BmrFI CCNGG 4 cut(s) 140, 1171, 1925, 2671
BpiI GAAGAC 3 cut(s) 141, 494, 2099
BplI GAGNNNNNCTC 2 cut(s) 256, 288
BpmI CTGGAG 2 cut(s) 833, 2354
Bpu10I CCTNAGC 1 cut(s) 1987
Bpu1102I GCTNAGC 1 cut(s) 1854
Bpu14I TTCGAA 1 cut(s) 2448
BpuEI CTTGAG 4 cut(s) 908, 1255, 1379, 1809
BpuMI CCSGG 2 cut(s) 140, 2671
BsaBI GATNNNNATC 2 cut(s) 396, 1868
BsaJI CCNNGG 8 cut(s) 791, 1170, 1244, 1329, 1765, 1832, 2056, 2496
BsaWI WCCGGW 1 cut(s) 1355
Bsc4I CCNNNNNNNGG 6 cut(s) 311, 797, 1553, 1849, 2377, 2670
Bse1I ACTGG 3 cut(s) 898, 1361, 1684
Bse21I CCTNAGG 1 cut(s) 260
Bse3DI GCAATG 2 cut(s) 643, 2463
Bse8I GATNNNNATC 2 cut(s) 396, 1868
BseBI CCWGG 2 cut(s) 1171, 1925
BseDI CCNNGG 8 cut(s) 791, 1170, 1244, 1329, 1765, 1832, 2056, 2496
BseGI GGATG 3 cut(s) 206, 667, 2066
BseJI GATNNNNATC 2 cut(s) 396, 1868
BseLI CCNNNNNNNGG 6 cut(s) 311, 797, 1553, 1849, 2377, 2670
BseMI GCAATG 2 cut(s) 643, 2463
BseMII CTCAG 8 cut(s) 267, 860, 1601, 1641, 1839, 1845, 1989, 2001
BseNI ACTGG 3 cut(s) 898, 1361, 1684
BseRI GAGGAG 2 cut(s) 29, 32
BseSI GKGCMC 1 cut(s) 1192
BseXI GCAGC 6 cut(s) 16, 91, 794, 1102, 1282, 2017
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 2 cut(s) 2016, 2369
Bsh1236I CGCG 2 cut(s) 77, 675
BshFI GGCC 3 cut(s) 790, 2307, 2674
BshNI GGYRCC 1 cut(s) 1784
BsiHKAI GWGCWC 5 cut(s) 118, 719, 934, 1192, 1860
BsiSI CCGG 3 cut(s) 140, 1356, 2670
BslFI GGGAC 3 cut(s) 589, 601, 2218
BslI CCNNNNNNNGG 6 cut(s) 311, 797, 1553, 1849, 2377, 2670
BsmAI GTCTC 1 cut(s) 2002
BsmFI GGGAC 3 cut(s) 589, 601, 2218
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 3 cut(s) 790, 2307, 2674
Bsp119I TTCGAA 1 cut(s) 2448
Bsp1286I GDGCHC 5 cut(s) 118, 719, 934, 1192, 1860
Bsp1407I TGTACA 1 cut(s) 2077
Bsp143I GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
Bsp1720I GCTNAGC 1 cut(s) 1854
Bsp19I CCATGG 3 cut(s) 1765, 2056, 2496
BspACI CCGC 3 cut(s) 554, 673, 1067
BspANI GGCC 3 cut(s) 790, 2307, 2674
BspCNI CTCAG 8 cut(s) 266, 859, 1602, 1640, 1840, 1846, 1988, 2000
BspFNI CGCG 2 cut(s) 77, 675
BspLI GGNNCC 2 cut(s) 1786, 1958
BspMAI CTGCAG 2 cut(s) 784, 2317
BspMI ACCTGC 3 cut(s) 256, 2201, 2320
BspPI GGATC 3 cut(s) 637, 1175, 1564
BspT104I TTCGAA 1 cut(s) 2448
BspT107I GGYRCC 1 cut(s) 1784
BspTI CTTAAG 3 cut(s) 1217, 1448, 2110
BsrDI GCAATG 2 cut(s) 643, 2463
BsrGI TGTACA 1 cut(s) 2077
BsrI ACTGG 3 cut(s) 898, 1361, 1684
BssECI CCNNGG 8 cut(s) 791, 1170, 1244, 1329, 1765, 1832, 2056, 2496
BssMI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
BssT1I CCWWGG 4 cut(s) 1329, 1765, 2056, 2496
Bst2UI CCWGG 2 cut(s) 1171, 1925
Bst4CI ACNGT 4 cut(s) 1907, 2197, 2237, 2526
BstAFI CTTAAG 3 cut(s) 1217, 1448, 2110
BstAUI TGTACA 1 cut(s) 2077
BstBI TTCGAA 1 cut(s) 2448
BstC8I GCNNGC 1 cut(s) 1368
BstDSI CCRYGG 3 cut(s) 1765, 2056, 2496
BstF5I GGATG 3 cut(s) 206, 667, 2066
BstFNI CGCG 2 cut(s) 77, 675
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 2 cut(s) 79, 84
BstKTI GATC 9 cut(s) 57, 394, 632, 1012, 1183, 1460, 1559, 2301, 2401
BstMAI GTCTC 1 cut(s) 2002
BstMBI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
BstMWI GCNNNNNNNGC 3 cut(s) 1230, 2027, 2135
BstNI CCWGG 2 cut(s) 1171, 1925
BstNSI RCATGY 1 cut(s) 2466
BstSCI CCNGG 4 cut(s) 138, 1169, 1923, 2669
BstSFI CTRYAG 4 cut(s) 780, 1228, 2233, 2313
BstSLI GKGCMC 1 cut(s) 1192
BstUI CGCG 2 cut(s) 77, 675
BstV1I GCAGC 6 cut(s) 16, 91, 794, 1102, 1282, 2017
BstV2I GAAGAC 3 cut(s) 141, 494, 2099
BstXI CCANNNNNNTGG 1 cut(s) 2200
Bsu36I CCTNAGG 1 cut(s) 260
BsuI GTATCC 3 cut(s) 136, 231, 1895
BsuRI GGCC 3 cut(s) 790, 2307, 2674
BtgI CCRYGG 3 cut(s) 1765, 2056, 2496
BtgZI GCGATG 2 cut(s) 2002, 2343
BtrI CACGTC 2 cut(s) 2185, 2266
BtsCI GGATG 3 cut(s) 206, 667, 2066
BtsI GCAGTG 1 cut(s) 2357
BtsIMutI CAGTG 5 cut(s) 717, 1368, 1728, 2357, 2531
BveI ACCTGC 3 cut(s) 256, 2201, 2320
Cac8I GCNNGC 1 cut(s) 1368
CaiI CAGNNNCTG 2 cut(s) 1367, 1466
CfoI GCGC 2 cut(s) 79, 84
Cfr13I GGNCC 3 cut(s) 1358, 1829, 2672
CspCI CAANNNNNGTGG 2 cut(s) 1264, 1299
DpnI GATC 9 cut(s) 56, 393, 631, 1011, 1182, 1459, 1558, 2300, 2400
DpnII GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
DraI TTTAAA 1 cut(s) 300
DraIII CACNNNGTG 1 cut(s) 2347
Ecl136II GAGCTC 2 cut(s) 116, 1858
Eco130I CCWWGG 4 cut(s) 1329, 1765, 2056, 2496
Eco147I AGGCCT 1 cut(s) 790
Eco24I GRGCYC 2 cut(s) 118, 1860
Eco47I GGWCC 2 cut(s) 1358, 1829
Eco53kI GAGCTC 2 cut(s) 116, 1858
Eco57I CTGAAG 3 cut(s) 327, 894, 2598
Eco81I CCTNAGG 1 cut(s) 260
EcoICRI GAGCTC 2 cut(s) 116, 1858
EcoRI GAATTC 3 cut(s) 1659, 2337, 2428
EcoRII CCWGG 2 cut(s) 1169, 1923
EcoT14I CCWWGG 4 cut(s) 1329, 1765, 2056, 2496
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 2 cut(s) 118, 1860
ErhI CCWWGG 4 cut(s) 1329, 1765, 2056, 2496
FaqI GGGAC 3 cut(s) 589, 601, 2218
FbaI TGATCA 2 cut(s) 2298, 2398
Fnu4HI GCNGC 6 cut(s) 30, 80, 783, 1091, 1296, 2031
FokI GGATG 3 cut(s) 193, 674, 2073
FriOI GRGCYC 2 cut(s) 118, 1860
Fsp4HI GCNGC 6 cut(s) 30, 80, 783, 1091, 1296, 2031
FspBI CTAG 7 cut(s) 620, 786, 1113, 1574, 2042, 2472, 2682
GlaI GCGC 2 cut(s) 78, 83
GluI GCNGC 6 cut(s) 30, 80, 783, 1091, 1296, 2031
GsaI CCCAGC 1 cut(s) 315
GsuI CTGGAG 2 cut(s) 833, 2354
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 3 cut(s) 790, 2307, 2674
HapII CCGG 3 cut(s) 140, 1356, 2670
HhaI GCGC 2 cut(s) 79, 84
Hin6I GCGC 2 cut(s) 77, 82
HinP1I GCGC 2 cut(s) 77, 82
HincII GTYRAC 2 cut(s) 24, 409
HindII GTYRAC 2 cut(s) 24, 409
HindIII AAGCTT 2 cut(s) 1310, 2442
HpaII CCGG 3 cut(s) 140, 1356, 2670
HphI GGTGA 9 cut(s) 153, 436, 717, 1004, 1095, 1190, 1705, 2368, 2610
Hpy166II GTNNAC 8 cut(s) 24, 73, 409, 806, 1190, 1762, 2080, 2618
Hpy8I GTNNAC 8 cut(s) 24, 73, 409, 806, 1190, 1762, 2080, 2618
Hpy99I CGWCG 2 cut(s) 2186, 2267
HpyAV CCTTC 9 cut(s) 43, 103, 708, 1058, 1205, 1625, 1645, 1649, 2405
HpyCH4III ACNGT 4 cut(s) 1907, 2197, 2237, 2526
HpyCH4IV ACGT 2 cut(s) 2184, 2265
HpyF10VI GCNNNNNNNGC 3 cut(s) 1230, 2027, 2135
HpySE526I ACGT 2 cut(s) 2184, 2265
HspAI GCGC 2 cut(s) 77, 82
Ksp22I TGATCA 2 cut(s) 2298, 2398
Kzo9I GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
LmnI GCTCC 6 cut(s) 113, 121, 814, 880, 1549, 1863
Lsp1109I GCAGC 6 cut(s) 16, 91, 794, 1102, 1282, 2017
MaeI CTAG 7 cut(s) 620, 786, 1113, 1574, 2042, 2472, 2682
MaeII ACGT 2 cut(s) 2184, 2265
MaeIII GTNAC 2 cut(s) 424, 959
MalI GATC 9 cut(s) 56, 393, 631, 1011, 1182, 1459, 1558, 2300, 2400
MboI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
MboII GAAGA 9 cut(s) 146, 494, 714, 755, 1130, 1391, 1543, 2104, 2591
MfeI CAATTG 1 cut(s) 1421
MhlI GDGCHC 5 cut(s) 118, 719, 934, 1192, 1860
MlyI GAGTC 4 cut(s) 830, 1334, 1393, 2368
MmeI TCCRAC 2 cut(s) 234, 1534
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 4 cut(s) 439, 1659, 2337, 2444
MslI CAYNNNNRTG 2 cut(s) 2085, 2198
MspA1I CMGCKG 1 cut(s) 1093
MspCI CTTAAG 3 cut(s) 1217, 1448, 2110
MspI CCGG 3 cut(s) 140, 1356, 2670
MspR9I CCNGG 4 cut(s) 140, 1171, 1925, 2671
MteI GCGCNGCGC 1 cut(s) 80
MunI CAATTG 1 cut(s) 1421
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 2 cut(s) 1171, 1925
MvnI CGCG 2 cut(s) 77, 675
MwoI GCNNNNNNNGC 3 cut(s) 1230, 2027, 2135
NciI CCSGG 2 cut(s) 140, 2671
NcoI CCATGG 3 cut(s) 1765, 2056, 2496
NdeII GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
NlaIV GGNNCC 2 cut(s) 1786, 1958
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 2466
NspV TTCGAA 1 cut(s) 2448
PceI AGGCCT 1 cut(s) 790
PctI GAATGC 1 cut(s) 847
PdmI GAANNNNTTC 4 cut(s) 439, 1659, 2337, 2444
PfeI GAWTC 9 cut(s) 707, 1100, 1417, 1749, 1869, 2406, 2551, 2645, 2660
PflMI CCANNNNNTGG 1 cut(s) 1553
PkrI GCNGC 6 cut(s) 31, 81, 784, 1092, 1297, 2032
PleI GAGTC 4 cut(s) 830, 1333, 1393, 2367
PpsI GAGTC 4 cut(s) 830, 1333, 1393, 2367
Psp124BI GAGCTC 2 cut(s) 118, 1860
Psp6I CCWGG 2 cut(s) 1169, 1923
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 1169, 1923
PspN4I GGNNCC 2 cut(s) 1786, 1958
PspPI GGNCC 3 cut(s) 1358, 1829, 2672
PstI CTGCAG 2 cut(s) 784, 2317
PstNI CAGNNNCTG 2 cut(s) 1367, 1466
PvuII CAGCTG 1 cut(s) 1093
RseI CAYNNNNRTG 2 cut(s) 2085, 2198
SacI GAGCTC 2 cut(s) 118, 1860
SatI GCNGC 6 cut(s) 30, 80, 783, 1091, 1296, 2031
Sau3AI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2298, 2398
Sau96I GGNCC 3 cut(s) 1358, 1829, 2672
ScaI AGTACT 4 cut(s) 606, 2048, 2231, 2286
SchI GAGTC 4 cut(s) 830, 1334, 1393, 2368
ScrFI CCNGG 4 cut(s) 140, 1171, 1925, 2671
SduI GDGCHC 5 cut(s) 118, 719, 934, 1192, 1860
SfcI CTRYAG 4 cut(s) 780, 1228, 2233, 2313
SfuI TTCGAA 1 cut(s) 2448
SinI GGWCC 2 cut(s) 1358, 1829
SmiMI CAYNNNNRTG 2 cut(s) 2085, 2198
SmlI CTYRAG 7 cut(s) 887, 1217, 1270, 1394, 1448, 1824, 2110
SmoI CTYRAG 7 cut(s) 887, 1217, 1270, 1394, 1448, 1824, 2110
SpeI ACTAGT 1 cut(s) 1573
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 3 cut(s) 554, 673, 1067
SspMI CTAG 7 cut(s) 620, 786, 1113, 1574, 2042, 2472, 2682
SstI GAGCTC 2 cut(s) 118, 1860
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 4 cut(s) 138, 1169, 1923, 2669
StyI CCWWGG 4 cut(s) 1329, 1765, 2056, 2496
TaaI ACNGT 4 cut(s) 1907, 2197, 2237, 2526
TaiI ACGT 2 cut(s) 2187, 2268
TaqI TCGA 8 cut(s) 180, 839, 1043, 2409, 2421, 2448, 2546, 2554
TaqII GACCGA 1 cut(s) 1846
TatI WGTACW 8 cut(s) 604, 864, 1477, 1941, 2046, 2077, 2229, 2284
TfiI GAWTC 9 cut(s) 707, 1100, 1417, 1749, 1869, 2406, 2551, 2645, 2660
TscAI CASTG 5 cut(s) 717, 1368, 1735, 2357, 2531
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 6 cut(s) 29, 79, 782, 1090, 1295, 2030
Tsp45I GTSAC 1 cut(s) 424
TspRI CASTG 5 cut(s) 717, 1368, 1735, 2357, 2531
Van91I CCANNNNNTGG 1 cut(s) 1553
Vha464I CTTAAG 3 cut(s) 1217, 1448, 2110
VneI GTGCAC 1 cut(s) 1188
VpaK11BI GGWCC 2 cut(s) 1358, 1829
XapI RAATTY 8 cut(s) 1198, 1405, 1513, 1659, 1720, 1882, 2337, 2428
XceI RCATGY 1 cut(s) 2466
XmnI GAANNNNTTC 4 cut(s) 439, 1659, 2337, 2444
XspI CTAG 7 cut(s) 620, 786, 1113, 1574, 2042, 2472, 2682
ZrmI AGTACT 4 cut(s) 606, 2048, 2231, 2286
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.