RLG00000016310

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005945
Physical Location & Seq
Reverse (-)
5596377 .. 5600512
4136 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016310

Sequence Viewer

Length: 2685 bp
ATGGACAGAGAAATGAAAAAGTCAACTGCTGCCTTCCTCCTCCTCTTTCTGATGATCTCTGAAAGTTCAGTTCACGCGCAGCGCCGAATAGCCTTCATAAGCATTGATTGTGGAGCTCCCTCAAAGCTGTATGAAGACCCGGATACCAACATAACTTACTCACCAGATGACAATTTCATAGACACAGGGATAAGCCAGAACATCTCATCCGAATATATGTATCCTAACAATCCAAACTTGCCATTACCTGCCTCAGACCTTAGGAGTTTTCCTCATGGAAACAAAAACTGCTACACTTTAAAACCTGAAGCTGGGAAAGGTAGTTTGAACCTAATCCGAGCTTCTTTCTTGTATGGAAACTACGATGGAAAAGACCAGCTACCGGAGTTTGATCTCTATCTTGATGTCAACTTCTGGTCTGTGGTGACATTTGGAAATGCTTCTGATATTGTCATAAAGGAGATTGTTGGTTTTGCACAATCTGATGTCATAAATGTTTGTCTTCTAAACAAAGGTTTAGGAACTCCTTTCATTTCGGCATTGGAACTTAGACCGCTTAACAGCTCAAATTATGGGACCGCGTTTGGGACTAATGCGTCATTAGTACTCTTTCAAAGACTAGACATTGGATCAAGCAATGGAACAGGTAGGTATGCCGAGGATGTTTATGACCGCATTTGGTCTAATTACATCTCTCCTTCTTGGGATTCAGTGAGCACTTCTTCACCAATTACTACTGATGAAGATGGATATAGAGTACCTTATGAAGTAATCAAGACTGCAGCTAGGCCTCGGAATGGAAGTGAACCTCTGGAGCTGTATTGGAACACAACTGACTCGAATGCTCAGTTTTATGTTTATATGTACTTTTCTGAAGTGGAGCATCTTGAGAAAAACCAGTCAAGAAAGTTGAACATATCTTGGAATGGTGCTCCATTGTTTGGACAATTTGTGCCTCGTTACTTATATGCAGACATATTATCTAACTCAAAATCTTTGGTGGGAAAAGATCACCAGATTTCTATATCTAAAGCAGAAAACTCGACCCTTCCACCGATTCTCAATGCGGTTGAGATTTACCAAGTCATGCAGCTGGTTGAATCACCAACTTCTAGTGAAGATGTTGATGCCATTATGAATGTCAAAACCACATATCAAATTAAAAAATCCTGGGCTGGTGATCCCTGTGCACCTAAGAATTTCTCTTGGGAAGGTCTTAAATGCAACTACAGCATTTCAGTTCCTCGGATTATTTCCCTGAACTTGACCTCAAGCAATTTGAGTGGGATTATAGCTGCTTCCATTTCCAAGCTTTTATCATTGGAGTCCTTGGACTTGTCAAACAATAACTTAACCGGACCAGTGCCTGCATTTCTGGAAGAACTAAAATCTCTCAAGACTCTGAATTTGAAAGGGAATCAATTGTCTGGTTTTGTTCCAAATGCACTTAAGAAAAGATCAGATGCTGGATTACTTGTACTAAGTGTGGATAGTCAAAATGTTTGTGGCTCAAATTCATGCAAAAAAAAGAAGAAAATTGTTGCTCCAATAGTTGGATCATTATTATCAGCACTAGTAGTCCTTATAGCTCTCATACTTGTCTGGAAGCTGAGAACACAAAAGAAATCAGGAGTGGAAACCTTTAAGAAGGAAGGAATTCCTATTGCATCAAAGAAGTGCCAGTTTAGTTATGATGAGGTTATAGAAATCACCAAAAATTTCAACACTGAAATCGGAAAAGGAGGATTCGGGGCTGTGTACCGTGGCTACATGAAAGATGGCACCCAAGTTGCAGTCAAAATGCTCTCTCCATCATCAACTCAAGGACCGAGGGAGTTCCAAACTGAGGCTGAGCTCCTGATGAGAATCCATCACAGAAATTTGGCATCATTTATTGGATACTGTGATGATGCTGATAACCTGGCTCTTATATATGAGTACATGGCTAATGGGAACCTTAAAGATTATCTCTCAGTTAAAAGCTCAGGTATGACTTGGGAATTGAGACTTCGCATCGCCATAGGTGCTGCACAAGGGCTAGAGTACTTACACCATGGATGCAATCCACCTATTGTACACAGAGATGTGAAGACTGCCAACATTCTCTTAAGTGAAAACTTGGAAGCTAAGATAGCAGATTTTGGTCTTTCCAAGGTTTTTTCCAGTGATAATGAAACCGACGTGGAAACCACAGTTATGGGGACAGCAGGTTATTTAGACCCAGAGTACTACAGTTCTCACAAGTTGAATGAAAAAAGCGACGTGTATAGTTTCGGGGTAGTACTATTGGAACTGATCACAGGCCAACCTGCAGTCATAAGAAGCGATGAACGAATCCACATTGTTCAGTGGGTGAGTCCTGAACTCCAGAAAGGGGATATAGCAAGTGTTGTTGATCAAAGAATTGAAGGAGATTTCGATGTGAATTCAGTTTGGAAAGCTTTCGAAATAGCAATGACATGCACAACTAGAACCTCTCAACAAAGAGCCACCATGGGTTTTGTGTTATCAGAGCTAAAACAGTGTTTGGAAATGGAGTTGTCGAGGAATCGAGAAAGAAATTCTGGATTGACTGAAGAAAGTAGAGACAGATTTGCAGCAGCATATAGTGGTTCACCATATAATAGTTCCATGTATGCAGATTCCACAAATGCTGATTCCATGACCGGGCCATTTGCTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

895

Amino Acids

99.08

Weight (kDa)

5.51

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 35 - 362 2.2e-108 Malectin-like domain
LRR_8 PF13855 419 - 475 4.5e-09 Leucine rich repeat
LRR_4 PF12799 440 - 476 9.9e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 574 - 837 3.6e-50 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 575 - 831 2.8e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 595
Acc36I ACCTGC 3 cut(s) 256, 2198, 2317
AccB1I GGYRCC 1 cut(s) 1781
AccB7I CCANNNNNTGG 2 cut(s) 941, 1553
AccII CGCG 2 cut(s) 77, 581
AciI CCGC 4 cut(s) 554, 579, 673, 1067
AclWI GGATC 3 cut(s) 637, 1175, 1564
AcsI RAATTY 8 cut(s) 1198, 1405, 1513, 1656, 1717, 1879, 2425, 2560
AcuI CTGAAG 3 cut(s) 327, 894, 2595
AfiI CCNNNNNNNGG 9 cut(s) 311, 382, 585, 797, 941, 1553, 1846, 2374, 2667
AflII CTTAAG 2 cut(s) 1448, 2107
AflIII ACRYGT 1 cut(s) 2262
AgsI TTSAA 8 cut(s) 328, 614, 913, 1100, 1411, 1723, 2248, 2408
AhlI ACTAGT 1 cut(s) 1573
AjiI CACGTC 2 cut(s) 2182, 2263
AjnI CCWGG 2 cut(s) 1169, 1920
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
Alw21I GWGCWC 5 cut(s) 118, 719, 934, 1192, 1857
Alw26I GTCTC 2 cut(s) 1999, 2580
Alw44I GTGCAC 1 cut(s) 1188
AlwI GGATC 3 cut(s) 637, 1175, 1564
AlwNI CAGNNNCTG 2 cut(s) 1367, 1466
AoxI GGCC 3 cut(s) 788, 2302, 2669
ApaLI GTGCAC 1 cut(s) 1188
ApeKI GCWGC 8 cut(s) 29, 79, 782, 1090, 1295, 2027, 2597, 2600
ApoI RAATTY 8 cut(s) 1198, 1405, 1513, 1656, 1717, 1879, 2425, 2560
ArsI GACNNNNNNTTYG 2 cut(s) 1390, 1422
Asp700I GAANNNNTTC 3 cut(s) 439, 1656, 2441
AspLEI GCGC 2 cut(s) 79, 84
AspS9I GGNCC 4 cut(s) 576, 1358, 1826, 2669
AsuC2I CCSGG 2 cut(s) 140, 2668
AsuHPI GGTGA 9 cut(s) 153, 436, 717, 1004, 1095, 1190, 1702, 2365, 2607
AsuII TTCGAA 1 cut(s) 2445
AvaII GGWCC 3 cut(s) 576, 1358, 1826
AxyI CCTNAGG 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 1192
BanI GGYRCC 1 cut(s) 1781
BanII GRGCYC 2 cut(s) 118, 1857
BbsI GAAGAC 3 cut(s) 141, 494, 2096
Bbv12I GWGCWC 5 cut(s) 118, 719, 934, 1192, 1857
BbvI GCAGC 8 cut(s) 16, 91, 794, 1102, 1282, 2014, 2609, 2612
BccI CCATC 5 cut(s) 359, 740, 1772, 1819, 1878
BcgI CGANNNNNNTGC 2 cut(s) 1023, 1057
BciT130I CCWGG 2 cut(s) 1171, 1922
BciVI GTATCC 3 cut(s) 136, 231, 1892
BclI TGATCA 2 cut(s) 2295, 2395
BcnI CCSGG 2 cut(s) 140, 2668
BcoDI GTCTC 2 cut(s) 1999, 2580
BcuI ACTAGT 1 cut(s) 1573
BfaI CTAG 7 cut(s) 620, 786, 1113, 1574, 2039, 2469, 2679
BfmI CTRYAG 4 cut(s) 780, 1228, 2230, 2310
BfoI RGCGCY 1 cut(s) 85
BfrI CTTAAG 2 cut(s) 1448, 2107
BfuAI ACCTGC 3 cut(s) 256, 2198, 2317
BfuI GTATCC 3 cut(s) 136, 231, 1892
BisI GCNGC 8 cut(s) 30, 80, 783, 1091, 1296, 2028, 2598, 2601
BlpI GCTNAGC 1 cut(s) 1851
BlsI GCNGC 8 cut(s) 31, 81, 784, 1092, 1297, 2029, 2599, 2602
BmcAI AGTACT 4 cut(s) 606, 2045, 2228, 2283
Bme1390I CCNGG 4 cut(s) 140, 1171, 1922, 2668
Bme18I GGWCC 3 cut(s) 576, 1358, 1826
BmgBI CACGTC 2 cut(s) 2182, 2263
BmgT120I GGNCC 4 cut(s) 576, 1358, 1826, 2669
BmiI GGNNCC 3 cut(s) 577, 1783, 1955
BmrFI CCNGG 4 cut(s) 140, 1171, 1922, 2668
BmsI GCATC 8 cut(s) 892, 1117, 1453, 1676, 1895, 1900, 2022, 2048
BpiI GAAGAC 3 cut(s) 141, 494, 2096
BplI GAGNNNNNCTC 2 cut(s) 256, 288
BpmI CTGGAG 2 cut(s) 833, 2351
Bpu10I CCTNAGC 1 cut(s) 1984
Bpu1102I GCTNAGC 1 cut(s) 1851
Bpu14I TTCGAA 1 cut(s) 2445
BpuEI CTTGAG 4 cut(s) 908, 1255, 1379, 1806
BpuMI CCSGG 2 cut(s) 140, 2668
BsaBI GATNNNNATC 2 cut(s) 396, 1865
BsaWI WCCGGW 2 cut(s) 382, 1355
Bsc4I CCNNNNNNNGG 9 cut(s) 311, 382, 585, 797, 941, 1553, 1846, 2374, 2667
Bse1I ACTGG 4 cut(s) 898, 1361, 1681, 2163
Bse21I CCTNAGG 1 cut(s) 260
Bse3DI GCAATG 2 cut(s) 643, 2460
Bse8I GATNNNNATC 2 cut(s) 396, 1865
BseBI CCWGG 2 cut(s) 1171, 1922
BseGI GGATG 3 cut(s) 206, 667, 2063
BseJI GATNNNNATC 2 cut(s) 396, 1865
BseLI CCNNNNNNNGG 9 cut(s) 311, 382, 585, 797, 941, 1553, 1846, 2374, 2667
BseMI GCAATG 2 cut(s) 643, 2460
BseMII CTCAG 7 cut(s) 267, 860, 1601, 1836, 1842, 1986, 1998
BseNI ACTGG 4 cut(s) 898, 1361, 1681, 2163
BseRI GAGGAG 2 cut(s) 29, 32
BseSI GKGCMC 1 cut(s) 1192
BseXI GCAGC 8 cut(s) 16, 91, 794, 1102, 1282, 2014, 2609, 2612
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 1 cut(s) 2013
Bsh1236I CGCG 2 cut(s) 77, 581
BshFI GGCC 3 cut(s) 790, 2304, 2671
BshNI GGYRCC 1 cut(s) 1781
BsiHKAI GWGCWC 5 cut(s) 118, 719, 934, 1192, 1857
BsiSI CCGG 4 cut(s) 140, 383, 1356, 2667
BslFI GGGAC 3 cut(s) 589, 601, 2215
BslI CCNNNNNNNGG 9 cut(s) 311, 382, 585, 797, 941, 1553, 1846, 2374, 2667
BsmAI GTCTC 2 cut(s) 1999, 2580
BsmFI GGGAC 3 cut(s) 589, 601, 2215
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 3 cut(s) 790, 2304, 2671
Bsp119I TTCGAA 1 cut(s) 2445
Bsp1286I GDGCHC 5 cut(s) 118, 719, 934, 1192, 1857
Bsp1407I TGTACA 1 cut(s) 2074
Bsp143I GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
Bsp1720I GCTNAGC 1 cut(s) 1851
Bsp19I CCATGG 2 cut(s) 2053, 2493
BspACI CCGC 4 cut(s) 554, 579, 673, 1067
BspANI GGCC 3 cut(s) 790, 2304, 2671
BspCNI CTCAG 7 cut(s) 266, 859, 1602, 1837, 1843, 1985, 1997
BspFNI CGCG 2 cut(s) 77, 581
BspLI GGNNCC 3 cut(s) 577, 1783, 1955
BspMAI CTGCAG 2 cut(s) 784, 2314
BspMI ACCTGC 3 cut(s) 256, 2198, 2317
BspPI GGATC 3 cut(s) 637, 1175, 1564
BspT104I TTCGAA 1 cut(s) 2445
BspT107I GGYRCC 1 cut(s) 1781
BspTI CTTAAG 2 cut(s) 1448, 2107
BsrDI GCAATG 2 cut(s) 643, 2460
BsrGI TGTACA 1 cut(s) 2074
BsrI ACTGG 4 cut(s) 898, 1361, 1681, 2163
BssMI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
BssT1I CCWWGG 4 cut(s) 1329, 2053, 2151, 2493
Bst2UI CCWGG 2 cut(s) 1171, 1922
Bst4CI ACNGT 5 cut(s) 1763, 1904, 2194, 2234, 2523
BstAFI CTTAAG 2 cut(s) 1448, 2107
BstAUI TGTACA 1 cut(s) 2074
BstBI TTCGAA 1 cut(s) 2445
BstC8I GCNNGC 1 cut(s) 1368
BstDSI CCRYGG 3 cut(s) 1762, 2053, 2493
BstF5I GGATG 3 cut(s) 206, 667, 2063
BstFNI CGCG 2 cut(s) 77, 581
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 2 cut(s) 79, 84
BstKTI GATC 9 cut(s) 57, 394, 632, 1012, 1183, 1460, 1559, 2298, 2398
BstMAI GTCTC 2 cut(s) 1999, 2580
BstMBI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
BstMWI GCNNNNNNNGC 3 cut(s) 1230, 2024, 2132
BstNI CCWGG 2 cut(s) 1171, 1922
BstNSI RCATGY 1 cut(s) 2463
BstSCI CCNGG 4 cut(s) 138, 1169, 1920, 2666
BstSFI CTRYAG 4 cut(s) 780, 1228, 2230, 2310
BstSLI GKGCMC 1 cut(s) 1192
BstUI CGCG 2 cut(s) 77, 581
BstV1I GCAGC 8 cut(s) 16, 91, 794, 1102, 1282, 2014, 2609, 2612
BstV2I GAAGAC 3 cut(s) 141, 494, 2096
BstXI CCANNNNNNTGG 1 cut(s) 2197
Bsu36I CCTNAGG 1 cut(s) 260
BsuI GTATCC 3 cut(s) 136, 231, 1892
BsuRI GGCC 3 cut(s) 790, 2304, 2671
BtgI CCRYGG 3 cut(s) 1762, 2053, 2493
BtgZI GCGATG 2 cut(s) 1999, 2340
BtrI CACGTC 2 cut(s) 2182, 2263
BtsCI GGATG 3 cut(s) 206, 667, 2063
BtsIMutI CAGTG 6 cut(s) 717, 1368, 1725, 2170, 2354, 2528
BveI ACCTGC 3 cut(s) 256, 2198, 2317
Cac8I GCNNGC 1 cut(s) 1368
CaiI CAGNNNCTG 2 cut(s) 1367, 1466
CfoI GCGC 2 cut(s) 79, 84
Cfr13I GGNCC 4 cut(s) 576, 1358, 1826, 2669
CseI GACGC 1 cut(s) 585
CspCI CAANNNNNGTGG 2 cut(s) 1264, 1299
DpnI GATC 9 cut(s) 56, 393, 631, 1011, 1182, 1459, 1558, 2297, 2397
DpnII GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
DraI TTTAAA 1 cut(s) 300
DrdI GACNNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 595
Ecl136II GAGCTC 2 cut(s) 116, 1855
Eco130I CCWWGG 4 cut(s) 1329, 2053, 2151, 2493
Eco147I AGGCCT 1 cut(s) 790
Eco24I GRGCYC 2 cut(s) 118, 1857
Eco47I GGWCC 3 cut(s) 576, 1358, 1826
Eco53kI GAGCTC 2 cut(s) 116, 1855
Eco57I CTGAAG 3 cut(s) 327, 894, 2595
Eco81I CCTNAGG 1 cut(s) 260
EcoICRI GAGCTC 2 cut(s) 116, 1855
EcoRI GAATTC 2 cut(s) 1656, 2425
EcoRII CCWGG 2 cut(s) 1169, 1920
EcoT14I CCWWGG 4 cut(s) 1329, 2053, 2151, 2493
EcoT38I GRGCYC 2 cut(s) 118, 1857
ErhI CCWWGG 4 cut(s) 1329, 2053, 2151, 2493
FaqI GGGAC 3 cut(s) 589, 601, 2215
FbaI TGATCA 2 cut(s) 2295, 2395
Fnu4HI GCNGC 8 cut(s) 30, 80, 783, 1091, 1296, 2028, 2598, 2601
FokI GGATG 3 cut(s) 193, 674, 2070
FriOI GRGCYC 2 cut(s) 118, 1857
Fsp4HI GCNGC 8 cut(s) 30, 80, 783, 1091, 1296, 2028, 2598, 2601
FspBI CTAG 7 cut(s) 620, 786, 1113, 1574, 2039, 2469, 2679
GlaI GCGC 2 cut(s) 78, 83
GluI GCNGC 8 cut(s) 30, 80, 783, 1091, 1296, 2028, 2598, 2601
GsaI CCCAGC 1 cut(s) 315
GsuI CTGGAG 2 cut(s) 833, 2351
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 3 cut(s) 790, 2304, 2671
HapII CCGG 4 cut(s) 140, 383, 1356, 2667
HgaI GACGC 1 cut(s) 585
HhaI GCGC 2 cut(s) 79, 84
Hin6I GCGC 2 cut(s) 77, 82
HinP1I GCGC 2 cut(s) 77, 82
HincII GTYRAC 2 cut(s) 24, 409
HindII GTYRAC 2 cut(s) 24, 409
HindIII AAGCTT 2 cut(s) 1310, 2439
HpaII CCGG 4 cut(s) 140, 383, 1356, 2667
HphI GGTGA 9 cut(s) 153, 436, 717, 1004, 1095, 1190, 1702, 2365, 2607
Hpy166II GTNNAC 8 cut(s) 24, 73, 409, 806, 1190, 1759, 2077, 2615
Hpy8I GTNNAC 8 cut(s) 24, 73, 409, 806, 1190, 1759, 2077, 2615
Hpy99I CGWCG 2 cut(s) 2183, 2264
HpyAV CCTTC 8 cut(s) 43, 103, 708, 1058, 1205, 1642, 1646, 2402
HpyCH4III ACNGT 5 cut(s) 1763, 1904, 2194, 2234, 2523
HpyCH4IV ACGT 2 cut(s) 2181, 2262
HpyF10VI GCNNNNNNNGC 3 cut(s) 1230, 2024, 2132
HpySE526I ACGT 2 cut(s) 2181, 2262
HspAI GCGC 2 cut(s) 77, 82
Ksp22I TGATCA 2 cut(s) 2295, 2395
Kzo9I GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
LmnI GCTCC 7 cut(s) 113, 121, 814, 880, 937, 1549, 1860
Lsp1109I GCAGC 8 cut(s) 16, 91, 794, 1102, 1282, 2014, 2609, 2612
LweI GCATC 8 cut(s) 892, 1117, 1453, 1676, 1895, 1900, 2022, 2048
MaeI CTAG 7 cut(s) 620, 786, 1113, 1574, 2039, 2469, 2679
MaeII ACGT 2 cut(s) 2181, 2262
MaeIII GTNAC 2 cut(s) 424, 959
MalI GATC 9 cut(s) 56, 393, 631, 1011, 1182, 1459, 1558, 2297, 2397
MboI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
MboII GAAGA 9 cut(s) 146, 494, 714, 755, 1130, 1391, 1543, 2101, 2588
MfeI CAATTG 1 cut(s) 1421
MhlI GDGCHC 5 cut(s) 118, 719, 934, 1192, 1857
MlyI GAGTC 4 cut(s) 830, 1334, 1393, 2365
MmeI TCCRAC 1 cut(s) 1534
MroXI GAANNNNTTC 3 cut(s) 439, 1656, 2441
MslI CAYNNNNRTG 2 cut(s) 2082, 2195
MspA1I CMGCKG 1 cut(s) 1093
MspCI CTTAAG 2 cut(s) 1448, 2107
MspI CCGG 4 cut(s) 140, 383, 1356, 2667
MspR9I CCNGG 4 cut(s) 140, 1171, 1922, 2668
MteI GCGCNGCGC 1 cut(s) 80
MunI CAATTG 1 cut(s) 1421
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 2 cut(s) 1171, 1922
MvnI CGCG 2 cut(s) 77, 581
MwoI GCNNNNNNNGC 3 cut(s) 1230, 2024, 2132
NciI CCSGG 2 cut(s) 140, 2668
NcoI CCATGG 2 cut(s) 2053, 2493
NdeII GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
NlaIV GGNNCC 3 cut(s) 577, 1783, 1955
NmeAIII GCCGAG 1 cut(s) 682
NmuCI GTSAC 1 cut(s) 424
NspI RCATGY 1 cut(s) 2463
NspV TTCGAA 1 cut(s) 2445
PceI AGGCCT 1 cut(s) 790
PctI GAATGC 1 cut(s) 847
PdmI GAANNNNTTC 3 cut(s) 439, 1656, 2441
PflMI CCANNNNNTGG 2 cut(s) 941, 1553
PkrI GCNGC 8 cut(s) 31, 81, 784, 1092, 1297, 2029, 2599, 2602
PleI GAGTC 4 cut(s) 830, 1333, 1393, 2364
PpsI GAGTC 4 cut(s) 830, 1333, 1393, 2364
Psp124BI GAGCTC 2 cut(s) 118, 1857
Psp6I CCWGG 2 cut(s) 1169, 1920
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 1169, 1920
PspN4I GGNNCC 3 cut(s) 577, 1783, 1955
PspPI GGNCC 4 cut(s) 576, 1358, 1826, 2669
PstI CTGCAG 2 cut(s) 784, 2314
PstNI CAGNNNCTG 2 cut(s) 1367, 1466
PvuII CAGCTG 1 cut(s) 1093
RseI CAYNNNNRTG 2 cut(s) 2082, 2195
SacI GAGCTC 2 cut(s) 118, 1857
SatI GCNGC 8 cut(s) 30, 80, 783, 1091, 1296, 2028, 2598, 2601
Sau3AI GATC 9 cut(s) 54, 391, 629, 1009, 1180, 1457, 1556, 2295, 2395
Sau96I GGNCC 4 cut(s) 576, 1358, 1826, 2669
ScaI AGTACT 4 cut(s) 606, 2045, 2228, 2283
SchI GAGTC 4 cut(s) 830, 1334, 1393, 2365
ScrFI CCNGG 4 cut(s) 140, 1171, 1922, 2668
SduI GDGCHC 5 cut(s) 118, 719, 934, 1192, 1857
SfaNI GCATC 8 cut(s) 892, 1117, 1453, 1676, 1895, 1900, 2022, 2048
SfcI CTRYAG 4 cut(s) 780, 1228, 2230, 2310
SfuI TTCGAA 1 cut(s) 2445
SinI GGWCC 3 cut(s) 576, 1358, 1826
SmiMI CAYNNNNRTG 2 cut(s) 2082, 2195
SmlI CTYRAG 6 cut(s) 887, 1270, 1394, 1448, 1821, 2107
SmoI CTYRAG 6 cut(s) 887, 1270, 1394, 1448, 1821, 2107
SpeI ACTAGT 1 cut(s) 1573
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 4 cut(s) 554, 579, 673, 1067
SspMI CTAG 7 cut(s) 620, 786, 1113, 1574, 2039, 2469, 2679
SstI GAGCTC 2 cut(s) 118, 1857
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 4 cut(s) 138, 1169, 1920, 2666
StyI CCWWGG 4 cut(s) 1329, 2053, 2151, 2493
TaaI ACNGT 5 cut(s) 1763, 1904, 2194, 2234, 2523
TaiI ACGT 2 cut(s) 2184, 2265
TaqI TCGA 6 cut(s) 839, 1043, 2418, 2445, 2543, 2551
TaqII GACCGA 1 cut(s) 1843
TatI WGTACW 8 cut(s) 604, 864, 1477, 1938, 2043, 2074, 2226, 2281
TscAI CASTG 6 cut(s) 717, 1368, 1732, 2170, 2354, 2528
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 8 cut(s) 29, 79, 782, 1090, 1295, 2027, 2597, 2600
Tsp45I GTSAC 1 cut(s) 424
TspRI CASTG 6 cut(s) 717, 1368, 1732, 2170, 2354, 2528
Van91I CCANNNNNTGG 2 cut(s) 941, 1553
Vha464I CTTAAG 2 cut(s) 1448, 2107
VneI GTGCAC 1 cut(s) 1188
VpaK11BI GGWCC 3 cut(s) 576, 1358, 1826
XapI RAATTY 8 cut(s) 1198, 1405, 1513, 1656, 1717, 1879, 2425, 2560
XceI RCATGY 1 cut(s) 2463
XmnI GAANNNNTTC 3 cut(s) 439, 1656, 2441
XspI CTAG 7 cut(s) 620, 786, 1113, 1574, 2039, 2469, 2679
ZrmI AGTACT 4 cut(s) 606, 2045, 2228, 2283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.