MD02G1073500.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008827
Physical Location & Seq
Reverse (-)
5909349 .. 5914793
5445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1073500.v1.1.491

Sequence Viewer

Length: 3921 bp
ATGCTTCACAGGAGCTTCAAGCCTGCCAAGTGCAAAACGTCATTGAAGCTTGCGGTTTCGCGGATAAAGCTATTGAAGAACAAGAAGGATGCACAGGTGAAGCAGATAAGGAGGGAATTGGCCCAGTTGCTTGAGACTGGCCAGGACCGGACTGCGAGAATTCGGGTTGAACATGTGGTCAGAGAAGAGAAGACAAAGGCAGCATTCGAACTTATCGAGATATATTGCGAGCTTATTGCCGCACGCTTGCCTATGATTGAATCACAAAAAAACTGCCCCATTGATCTGAAGGAAGCAATTACAAGTGTAGTATTTGCATCTCCAAGATGCGCAGACATACCAGAACTCATGGATGTTCGCAAGCATTTCACAGCTAAATATGGAAAGGAGTTTATCTCTAACGCTGTTGAATTGCGCCCAGATTGTGGCGTAAGTCGCATGTTAGTGGAGAAGTTATCTGCCAAATCACCCGATGGCCCTGCCAAGATGAAAATCTTGGCTGCAATAGCAGAGGAACATAACGTCAAATGGGATCCTGAAGCACTTGAAGAGAAAGAGTCTAAGCCTCCAGAGGACTTGCTGAATGGGCCAAATACTTTTGGTAGAATTCCTGCGGAACCTTATCTTGGCCCACCTCCTCCTAATCATGATGTCAAGGCACCTTCTAATGCTCAGGTTCCACCTCAAAATTATGGGAATTATGGTCCACCCTCGAACTTTAACGAGCAGAATGCAAGATCTTCACCTCACTTTCAGGATTCTGCTTCTTCAAATGTTTCTTCCAACAAAGCAACCACATCTGGCTCATTTCATTCAGAAGTTAGATCTTCAGGAAATCAAAATGATGGGATGGAAAATAGGCTTTTGTCTCCTGAGGATGGAAGTTCTTCTTCACCGGGTAGGCAGAGCTGGAACATGAATTTTAAGGATGCTGCTTCTGCAGCACAGGCAGCTGCTGAATCAGCAGAAATGGCAAGCATGGCTGCCAGAGCAGCTGCTGAACTTTCAAGAAAACATTCTTCAGAGTCACGGGATAAGTTTAGCCAGTCAGCTTCCTTAGCTTCCAGTAGAACTTCCACAAATGCTCCTTCTGCGAGTAGTCACCAAATGCAAGATCGATATCCTCACCAAATGGCAGACCGAGATTCTCAAAATAAGTCATCTGAGACCGAGAAGCGTGATTTCACAGATGAAGTGCGTATGAAGAGACAGTCTAGCAACACTAATGTGCAAGCAAGTGAGATGCAAAGTGGAGTGAAACCAGATAATATTTCGCATTTTGACAATGCAAGATCTGAAGAGAAATCTAGTAGGCCATCCTCCCATTCTAATTCTATGGGAGAAGTGAGTACGAAGAGACAGTCAAGCAACACTGATGTGACGTATGGGAGTGAGATGCAAACTGGGAACACACCTGCTAACATGTCTTATTTTGGCGGTATGAGATCTGAAGAAAAATCTAGTAGGCCTTCCTCCCGATCTAATTCAAGCTTTGGTAGTGAAGATCAGGAAGATGTTTTAAGAGGGAACTTGGAAGATATGAGGACTGGAGAACAATCTATGAGGTCCTCCTCTCATTCTCATTCAAGAAATTTTAGTAATGACCATGAAAATGTTTCAAATGGCTCTGTTGAGGAGTCTTTTGTTAACGATGTTAACATCTTCCAAAGCACAAAGGAAACAAATTCTTATGGAAATGCTGCTTTGGTTTTTGATGACTCTGGTTCGGATGATGATAAAAGTAAATTTGAGGTGGAGGACAATAAGGGGCAAGGATCTAGTTTGTTTTTTCCTTCCCCTGATAGAAACTCATTCTCTCCTTCAGCAGATTTAGATCCTTGGAGCTCTAGACAACAGACGGATGAAGTACGATTTAAGTCCAGTTCACAATTAAGTTCTTCATCAGTGAAGCACTTGCCTCCTGTTTTTTCAGAAAATTTGGCAGGTTCCATGGACTCTCCACAACCAACTGGTTTGTTGCCTGTTGCTTTCGATGCTTCAGATGGCCCAAGTTCAGACAGTGAAGAGGAGTTAGACAAGCTTGCCCAGAGCACAGTTTCTAAAATTTCTCCTATGCAGAGCCAAAGTGCAAGTCATAGGTCATTAGGATCTTCATCCTCGGAGGAGATATATGTGGGTTCTAACAGAAAGCCAGGGTTACTGCCTTCTTTTCGTAATTTGGAATCTGCTGAAGTACCTCCTGAGAGAAGCCAAAGAGTCGAAAATAGAACCGTCCAGGAGGAAAACTATGATTTTGATGAATTGCCGACTGGTCGGCCATCTGTAACGCTAAGGAAATCTGGATTAGATTCAAATGCCAGGGACGATGTTCAAGCATCAAATTTGCGTCAAACTATGAAAGATAGTGAGATTTCAGAGGGATGTAGTTGTGTAGAAGACACTGACAATGAGCTGAGCTTAGGGATGTTGACAGGTGGGCTGCGAAATAAGGGTTTAAGGCATCCACCGTATACAAAGAAACTATCAGGAAGCTCTTCGTCTGTCAAACAAGATACTGGAGATACCAAGGTTGAGCAACCTTCCTTCCTTCCATCAGTCAGGACTTCTGTTGTCTCTGAAAGGGAGCAAGGGAGCAAAAGACTGATCAAGGAACGGAGCACACGAACCTCCGTCTCATATGTGGCTTCTGATGATGATTCTTCAGAGGATGAATTGTCACATAGAACTGTTAGAAGTAGCCAAGACTCGATAAAACTGATTCAGGAACGGAGTGCAAGAACCCCCGTCTCATATGTGGCTTCTGATGATGATTCTTCAGAGGATGAAGTGTCACATAGAACTGTTAGAAATAGCCAAAACTCGTTCAATAAAAAATTGGGACCTGAAGTAAAGGCAAGCTCCAAATCAACGTTTTCATTCTTTGATTCAGAGGATAGTGAGCCGGAGGAAGATCTTCTCACAAAGATTTCCACTAGCAATGCTCGTCCAAGTGCTAGACTGTCTAGAAGAACACAGCCGTCTTCAAATTCTGTTAGAAGTTCTAGCTCAAAGACAACAGTTGTGTCTGACGTGTCAGGGACTTCAGACTATGGAAAACCCTCTTCAAAGAGTTCCTATGCCACTGAAATTCAACTAAAGCCCTCGTCCCAGACCAAGAGCTCAGATCAGGAGTGGAATAGACTAGTAGAACAAGCGGCTCCTGGACCGGTACGAGAATCCAAGAGGGTCTTGCCCGTAGAGACTTCAAATTCAAACAGAAACTCCTCTTTGAGGAGTTTTAATTCCAATGAAACTTTACCAAAGCCGTTGTCCCAGACCAAGAGCTCAGAACGCCCAAGAAGTCAAGAGTGGCATAAACCAGTAGAACAAGTTGTTCCTAAACCAATGCCAGAGTCCAAGAGATCCTCACTTGTGGAGACTTCAAAGTCACATGGAAACCCCTCGTCAAGGAGTTCATATGCCACTGAAAGTCTACCAAAGCCCTTGTCCCAGACCAGAAGCTCAGAGCACCCAGGAAGTCAGGAGTGGCATAGACCGGCAGAACAAGCAGCTCCTAAACCAATTCGAGAATCCAAGAGGTCTTCACCGGAAGATGTAAAGACTTCTATGTCATTTGGAAACTCCTCTTCGAGGACTTCATATGCCACCGAGACTCTACCAAATCCCTCGTCCCAGACCAAGAGTTTGGAGCGCCCAGGAAGACAGGAGCGGCAAACACCAGTAGAACAAGCAGCGCCTAAACCAGTTCTGGAATCCAGGAGGTCCTCCTCGCATGTAGAGACTTCAAAGCCATTTGCAAGGGAGCAATCATCCGAAATTTCTCAAAAGATTGAAACATCTAAGTCCTCAGGTTCTACAGAGAGTTCATCAAAGGAGAAAGCTTCTCATGTCCACCCAAAGCTTCCAGACTCTGATGACATAATCGCCAAGTTTATGGCTCTTCGGAAGAATCGACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1307

Amino Acids

143.6

Weight (kDa)

6.51

Isoelectric Point (pI)

70.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 1.3e-59 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1423
AasI GACNNNNNNGTC 2 cut(s) 3385, 3568
Acc16I TGCGCA 1 cut(s) 331
Acc36I ACCTGC 2 cut(s) 1423, 1934
AccB1I GGYRCC 1 cut(s) 658
AccB7I CCANNNNNTGG 1 cut(s) 425
AccBSI CCGCTC 1 cut(s) 3670
AccI GTMKAC 2 cut(s) 2471, 3433
AccII CGCG 1 cut(s) 61
AciI CCGC 7 cut(s) 53, 61, 240, 614, 1437, 3155, 3670
AclI AACGTT 1 cut(s) 2870
AclWI GGATC 6 cut(s) 527, 540, 1783, 1829, 2116, 3357
AcoI YGGCCR 2 cut(s) 139, 2276
AfaI GTAC 4 cut(s) 1351, 1869, 2196, 3171
AfiI CCNNNNNNNGG 6 cut(s) 425, 626, 878, 3231, 3408, 3591
AflIII ACRYGT 3 cut(s) 172, 1422, 3030
AgeI ACCGGT 1 cut(s) 3166
AhlI ACTAGT 1 cut(s) 3142
AjiI CACGTC 1 cut(s) 3031
AjnI CCWGG 8 cut(s) 141, 2152, 2235, 2318, 3160, 3472, 3655, 3716
AjuI GAANNNNNNNTTGG 2 cut(s) 609, 641
AleI CACNNNNGTG 2 cut(s) 1226, 1376
Alw21I GWGCWC 6 cut(s) 1847, 2054, 2621, 3122, 3287, 3471
AlwI GGATC 6 cut(s) 527, 540, 1783, 1829, 2116, 3357
AlwNI CAGNNNCTG 3 cut(s) 956, 998, 3872
AoxI GGCC 9 cut(s) 120, 139, 475, 587, 628, 1313, 1466, 2005, 2276
AsiGI ACCGGT 1 cut(s) 3166
Asp700I GAANNNNTTC 4 cut(s) 886, 1015, 2494, 2913
AspLEI GCGC 4 cut(s) 332, 417, 3654, 3697
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 7 cut(s) 109, 459, 735, 885, 1094, 1118, 3537
AsuII TTCGAA 1 cut(s) 207
AvaII GGWCC 6 cut(s) 145, 704, 1566, 2840, 3164, 3723
AxyI CCTNAGG 2 cut(s) 873, 3808
BaeI ACNNNNGTAYC 2 cut(s) 2514, 2547
BalI TGGCCA 1 cut(s) 141
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 658
BanII GRGCYC 3 cut(s) 1847, 3122, 3287
BbsI GAAGAC 5 cut(s) 197, 2403, 2973, 3534, 3667
Bbv12I GWGCWC 6 cut(s) 1847, 2054, 2621, 3122, 3287, 3471
BccI CCATC 8 cut(s) 467, 839, 844, 872, 1324, 1997, 2287, 2560
BceAI ACGGC 2 cut(s) 2962, 3250
BcgI CGANNNNNNTGC 6 cut(s) 218, 252, 461, 495, 713, 747
BciT130I CCWGG 8 cut(s) 143, 2154, 2237, 2320, 3162, 3474, 3657, 3718
BclI TGATCA 1 cut(s) 2604
BcnI CCSGG 1 cut(s) 897
BcuI ACTAGT 1 cut(s) 3142
BfmI CTRYAG 2 cut(s) 939, 3816
BfoI RGCGCY 2 cut(s) 3655, 3698
BfuAI ACCTGC 2 cut(s) 1423, 1934
BglII AGATCT 5 cut(s) 737, 824, 1292, 1445, 2911
BlpI GCTNAGC 1 cut(s) 2414
Bme1390I CCNGG 9 cut(s) 143, 897, 2154, 2237, 2320, 3162, 3474, 3657, 3718
Bme18I GGWCC 6 cut(s) 145, 704, 1566, 2840, 3164, 3723
BmgBI CACGTC 1 cut(s) 3031
BmiI GGNNCC 7 cut(s) 534, 618, 660, 678, 1948, 2841, 3159
BmrFI CCNGG 9 cut(s) 143, 897, 2154, 2237, 2320, 3162, 3474, 3657, 3718
BmrI ACTGGG 2 cut(s) 118, 1413
BmsI GCATC 9 cut(s) 79, 317, 326, 919, 1233, 1386, 1984, 2345, 2470
BmuI ACTGGG 2 cut(s) 118, 1413
BpiI GAAGAC 5 cut(s) 197, 2403, 2973, 3534, 3667
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 3 cut(s) 552, 1569, 2538
Bpu10I CCTNAGC 4 cut(s) 672, 1057, 2291, 2419
Bpu1102I GCTNAGC 1 cut(s) 2414
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 897
Bsa29I ATCGAT 1 cut(s) 1117
BsaBI GATNNNNATC 4 cut(s) 491, 1119, 1833, 2113
BsaI GGTCTC 1 cut(s) 1160
BsaJI CCNNGG 8 cut(s) 1838, 1950, 2118, 2153, 2319, 2526, 3472, 3655
BsaWI WCCGGW 3 cut(s) 147, 3166, 3547
BsaXI ACNNNNNCTCC 4 cut(s) 1069, 1099, 3206, 3236
Bsc4I CCNNNNNNNGG 6 cut(s) 425, 626, 878, 3231, 3408, 3591
Bse118I RCCGGY 2 cut(s) 3166, 3496
Bse21I CCTNAGG 2 cut(s) 873, 3808
Bse3DI GCAATG 1 cut(s) 2944
Bse8I GATNNNNATC 4 cut(s) 491, 1119, 1833, 2113
BseBI CCWGG 8 cut(s) 143, 2154, 2237, 2320, 3162, 3474, 3657, 3718
BseCI ATCGAT 1 cut(s) 1117
BseDI CCNNGG 8 cut(s) 1838, 1950, 2118, 2153, 2319, 2526, 3472, 3655
BseJI GATNNNNATC 4 cut(s) 491, 1119, 1833, 2113
BseLI CCNNNNNNNGG 6 cut(s) 425, 626, 878, 3231, 3408, 3591
BseMI GCAATG 1 cut(s) 2944
BseMII CTCAG 9 cut(s) 686, 864, 1155, 2193, 2405, 3135, 3300, 3477, 3822
BseRI GAGGAG 9 cut(s) 627, 1561, 1649, 2042, 2138, 3214, 3247, 3574, 3718
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 9 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007, 2278
BshNI GGYRCC 1 cut(s) 658
BshTI ACCGGT 1 cut(s) 3166
BshVI ATCGAT 1 cut(s) 1117
BsiHKAI GWGCWC 6 cut(s) 1847, 2054, 2621, 3122, 3287, 3471
BsiSI CCGG 6 cut(s) 148, 896, 2903, 3167, 3497, 3548
BslFI GGGAC 7 cut(s) 2336, 2853, 3052, 3091, 3256, 3433, 3616
BslI CCNNNNNNNGG 6 cut(s) 425, 626, 878, 3231, 3408, 3591
BsmBI CGTCTC 2 cut(s) 2638, 2752
BsmFI GGGAC 7 cut(s) 2336, 2853, 3052, 3091, 3256, 3433, 3616
BsmI GAATGC 2 cut(s) 203, 736
BsnI GGCC 9 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007, 2278
Bso31I GGTCTC 1 cut(s) 1160
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 6 cut(s) 1847, 2054, 2621, 3122, 3287, 3471
Bsp1720I GCTNAGC 1 cut(s) 2414
Bsp19I CCATGG 1 cut(s) 1950
BspACI CCGC 7 cut(s) 53, 61, 240, 614, 1437, 3155, 3670
BspANI GGCC 9 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007, 2278
BspCNI CTCAG 9 cut(s) 685, 865, 1156, 2194, 2406, 3134, 3299, 3476, 3821
BspDI ATCGAT 1 cut(s) 1117
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 7 cut(s) 534, 618, 660, 678, 1948, 2841, 3159
BspMAI CTGCAG 1 cut(s) 943
BspMI ACCTGC 2 cut(s) 1423, 1934
BspPI GGATC 6 cut(s) 527, 540, 1783, 1829, 2116, 3357
BspQI GCTCTTC 2 cut(s) 2500, 3906
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 1160
BsrBI CCGCTC 1 cut(s) 3670
BsrDI GCAATG 1 cut(s) 2944
BsrFI RCCGGY 2 cut(s) 3166, 3496
BssAI RCCGGY 2 cut(s) 3166, 3496
BssECI CCNNGG 8 cut(s) 1838, 1950, 2118, 2153, 2319, 2526, 3472, 3655
BssNAI GTATAC 1 cut(s) 2472
BssT1I CCWWGG 3 cut(s) 1838, 1950, 2526
Bst1107I GTATAC 1 cut(s) 2472
Bst2UI CCWGG 8 cut(s) 143, 2154, 2237, 2320, 3162, 3474, 3657, 3718
BstBI TTCGAA 1 cut(s) 207
BstDSI CCRYGG 1 cut(s) 1950
BstENI CCTNNNNNAGG 3 cut(s) 3229, 3406, 3589
BstFNI CGCG 1 cut(s) 61
BstH2I RGCGCY 2 cut(s) 3655, 3698
BstHHI GCGC 4 cut(s) 332, 417, 3654, 3697
BstNI CCWGG 8 cut(s) 143, 2154, 2237, 2320, 3162, 3474, 3657, 3718
BstNSI RCATGY 4 cut(s) 176, 442, 1426, 3737
BstSCI CCNGG 9 cut(s) 141, 895, 2152, 2235, 2318, 3160, 3472, 3655, 3716
BstSFI CTRYAG 2 cut(s) 939, 3816
BstUI CGCG 1 cut(s) 61
BstV2I GAAGAC 5 cut(s) 197, 2403, 2973, 3534, 3667
BstXI CCANNNNNNTGG 2 cut(s) 3646, 3895
BstZ17I GTATAC 1 cut(s) 2472
Bsu15I ATCGAT 1 cut(s) 1117
Bsu36I CCTNAGG 2 cut(s) 873, 3808
BsuRI GGCC 9 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007, 2278
BsuTUI ATCGAT 1 cut(s) 1117
BtgI CCRYGG 1 cut(s) 1950
BtrI CACGTC 1 cut(s) 3031
BtsIMutI CAGTG 6 cut(s) 1371, 1911, 2026, 2400, 3081, 3423
BveI ACCTGC 2 cut(s) 1423, 1934
CaiI CAGNNNCTG 3 cut(s) 956, 998, 3872
CciI TCATGA 1 cut(s) 646
CfoI GCGC 4 cut(s) 332, 417, 3654, 3697
Cfr10I RCCGGY 2 cut(s) 3166, 3496
ClaI ATCGAT 1 cut(s) 1117
CseI GACGC 1 cut(s) 2336
Csp6I GTAC 4 cut(s) 1350, 1868, 2195, 3170
CspAI ACCGGT 1 cut(s) 3166
CviQI GTAC 4 cut(s) 1350, 1868, 2195, 3170
DrdI GACNNNNNNGTC 2 cut(s) 3385, 3568
DseDI GACNNNNNNGTC 2 cut(s) 3385, 3568
EaeI YGGCCR 2 cut(s) 139, 2276
Ecl136II GAGCTC 3 cut(s) 1845, 3120, 3285
Eco130I CCWWGG 3 cut(s) 1838, 1950, 2526
Eco147I AGGCCT 1 cut(s) 1468
Eco24I GRGCYC 3 cut(s) 1847, 3122, 3287
Eco31I GGTCTC 1 cut(s) 1160
Eco32I GATATC 1 cut(s) 1121
Eco47I GGWCC 6 cut(s) 145, 704, 1566, 2840, 3164, 3723
Eco53kI GAGCTC 3 cut(s) 1845, 3120, 3285
Eco81I CCTNAGG 2 cut(s) 873, 3808
EcoICRI GAGCTC 3 cut(s) 1845, 3120, 3285
EcoNI CCTNNNNNAGG 3 cut(s) 3229, 3406, 3589
EcoO109I RGGNCCY 3 cut(s) 1566, 2840, 3723
EcoRI GAATTC 2 cut(s) 159, 606
EcoRII CCWGG 8 cut(s) 141, 2152, 2235, 2318, 3160, 3472, 3655, 3716
EcoRV GATATC 1 cut(s) 1121
EcoT14I CCWWGG 3 cut(s) 1838, 1950, 2526
EcoT38I GRGCYC 3 cut(s) 1847, 3122, 3287
ErhI CCWWGG 3 cut(s) 1838, 1950, 2526
Esp3I CGTCTC 2 cut(s) 2638, 2752
FalI AAGNNNNNCTT 4 cut(s) 874, 906, 3173, 3205
FaqI GGGAC 7 cut(s) 2336, 2853, 3052, 3091, 3256, 3433, 3616
FauNDI CATATG 4 cut(s) 2638, 2752, 3418, 3601
FbaI TGATCA 1 cut(s) 2604
FblI GTMKAC 2 cut(s) 2471, 3433
FriOI GRGCYC 3 cut(s) 1847, 3122, 3287
FspI TGCGCA 1 cut(s) 331
GlaI GCGC 4 cut(s) 331, 416, 3653, 3696
GsuI CTGGAG 3 cut(s) 552, 1569, 2538
HaeII RGCGCY 2 cut(s) 3655, 3698
HaeIII GGCC 9 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007, 2278
HapII CCGG 6 cut(s) 148, 896, 2903, 3167, 3497, 3548
HgaI GACGC 1 cut(s) 2336
HhaI GCGC 4 cut(s) 332, 417, 3654, 3697
Hin6I GCGC 4 cut(s) 330, 415, 3652, 3695
HinP1I GCGC 4 cut(s) 330, 415, 3652, 3695
HincII GTYRAC 3 cut(s) 1648, 1657, 2430
HindII GTYRAC 3 cut(s) 1648, 1657, 2430
HindIII AAGCTT 5 cut(s) 47, 1489, 2039, 3840, 3860
HpaI GTTAAC 2 cut(s) 1648, 1657
HpaII CCGG 6 cut(s) 148, 896, 2903, 3167, 3497, 3548
HphI GGTGA 7 cut(s) 109, 459, 735, 885, 1094, 1118, 3537
Hpy166II GTNNAC 8 cut(s) 707, 1648, 1657, 1886, 2430, 2472, 3434, 3853
Hpy8I GTNNAC 8 cut(s) 707, 1648, 1657, 1886, 2430, 2472, 3434, 3853
HpyCH4IV ACGT 5 cut(s) 38, 522, 1382, 2870, 3030
HpySE526I ACGT 5 cut(s) 38, 522, 1382, 2870, 3030
HspAI GCGC 4 cut(s) 330, 415, 3652, 3695
Ksp22I TGATCA 1 cut(s) 2604
KspAI GTTAAC 2 cut(s) 1648, 1657
LguI GCTCTTC 2 cut(s) 2500, 3906
LweI GCATC 9 cut(s) 79, 317, 326, 919, 1233, 1386, 1984, 2345, 2470
MaeII ACGT 5 cut(s) 38, 522, 1382, 2870, 3030
MaeIII GTNAC 8 cut(s) 1026, 1100, 1378, 2157, 2284, 2676, 2790, 3387
MbiI CCGCTC 1 cut(s) 3670
MhlI GDGCHC 6 cut(s) 1847, 2054, 2621, 3122, 3287, 3471
MlsI TGGCCA 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 807
Mox20I TGGCCA 1 cut(s) 141
MroXI GAANNNNTTC 4 cut(s) 886, 1015, 2494, 2913
MscI TGGCCA 1 cut(s) 141
MseI TTAA 9 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456, 3240
MslI CAYNNNNRTG 4 cut(s) 443, 1226, 1376, 1611
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 2 cut(s) 953, 995
MspI CCGG 6 cut(s) 148, 896, 2903, 3167, 3497, 3548
MspR9I CCNGG 9 cut(s) 143, 897, 2154, 2237, 2320, 3162, 3474, 3657, 3718
Mva1269I GAATGC 2 cut(s) 203, 736
MvaI CCWGG 8 cut(s) 143, 2154, 2237, 2320, 3162, 3474, 3657, 3718
MvnI CGCG 1 cut(s) 61
NciI CCSGG 1 cut(s) 897
NcoI CCATGG 1 cut(s) 1950
NdeI CATATG 4 cut(s) 2638, 2752, 3418, 3601
NlaIV GGNNCC 7 cut(s) 534, 618, 660, 678, 1948, 2841, 3159
NmuCI GTSAC 6 cut(s) 1026, 1100, 1378, 2676, 2790, 3387
NsbI TGCGCA 1 cut(s) 331
NspI RCATGY 4 cut(s) 176, 442, 1426, 3737
NspV TTCGAA 1 cut(s) 207
OliI CACNNNNGTG 2 cut(s) 1226, 1376
PagI TCATGA 1 cut(s) 646
PaqCI CACCTGC 1 cut(s) 1423
PceI AGGCCT 1 cut(s) 1468
PciI ACATGT 2 cut(s) 172, 1422
PciSI GCTCTTC 2 cut(s) 2500, 3906
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 2620, 3910
PctI GAATGC 2 cut(s) 203, 736
PdmI GAANNNNTTC 4 cut(s) 886, 1015, 2494, 2913
PflMI CCANNNNNTGG 1 cut(s) 425
PfoI TCCNGGA 3 cut(s) 2235, 3160, 3716
PinAI ACCGGT 1 cut(s) 3166
PpuMI RGGWCCY 3 cut(s) 1566, 2840, 3723
PscI ACATGT 2 cut(s) 172, 1422
Psp124BI GAGCTC 3 cut(s) 1847, 3122, 3287
Psp1406I AACGTT 1 cut(s) 2870
Psp5II RGGWCCY 3 cut(s) 1566, 2840, 3723
Psp6I CCWGG 8 cut(s) 141, 2152, 2235, 2318, 3160, 3472, 3655, 3716
PspGI CCWGG 8 cut(s) 141, 2152, 2235, 2318, 3160, 3472, 3655, 3716
PspN4I GGNNCC 7 cut(s) 534, 618, 660, 678, 1948, 2841, 3159
PspPPI RGGWCCY 3 cut(s) 1566, 2840, 3723
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 3 cut(s) 956, 998, 3872
PvuII CAGCTG 2 cut(s) 953, 995
RsaI GTAC 4 cut(s) 1351, 1869, 2196, 3171
RsaNI GTAC 4 cut(s) 1350, 1868, 2195, 3170
RseI CAYNNNNRTG 4 cut(s) 443, 1226, 1376, 1611
SacI GAGCTC 3 cut(s) 1847, 3122, 3287
SapI GCTCTTC 2 cut(s) 2500, 3906
SaqAI TTAA 9 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456, 3240
ScrFI CCNGG 9 cut(s) 143, 897, 2154, 2237, 2320, 3162, 3474, 3657, 3718
SduI GDGCHC 6 cut(s) 1847, 2054, 2621, 3122, 3287, 3471
SfaNI GCATC 9 cut(s) 79, 317, 326, 919, 1233, 1386, 1984, 2345, 2470
SfcI CTRYAG 2 cut(s) 939, 3816
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 6 cut(s) 145, 704, 1566, 2840, 3164, 3723
SmiMI CAYNNNNRTG 4 cut(s) 443, 1226, 1376, 1611
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SpeI ACTAGT 1 cut(s) 3142
SseBI AGGCCT 1 cut(s) 1468
SsiI CCGC 7 cut(s) 53, 61, 240, 614, 1437, 3155, 3670
SspI AATATT 1 cut(s) 1270
SstI GAGCTC 3 cut(s) 1847, 3122, 3287
StuI AGGCCT 1 cut(s) 1468
StyD4I CCNGG 9 cut(s) 141, 895, 2152, 2235, 2318, 3160, 3472, 3655, 3716
StyI CCWWGG 3 cut(s) 1838, 1950, 2526
TaiI ACGT 5 cut(s) 41, 525, 1385, 2873, 3033
TaqII GACCGA 2 cut(s) 1155, 1184
TauI GCSGC 3 cut(s) 242, 3158, 3673
Tru1I TTAA 9 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456, 3240
Tru9I TTAA 9 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456, 3240
TscAI CASTG 6 cut(s) 1378, 1911, 2026, 2407, 3088, 3430
TseFI GTSAC 6 cut(s) 1026, 1100, 1378, 2676, 2790, 3387
Tsp45I GTSAC 6 cut(s) 1026, 1100, 1378, 2676, 2790, 3387
TspGWI ACGGA 4 cut(s) 1874, 2620, 2629, 2743
TspRI CASTG 6 cut(s) 1378, 1911, 2026, 2407, 3088, 3430
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 6 cut(s) 145, 704, 1566, 2840, 3164, 3723
XagI CCTNNNNNAGG 3 cut(s) 3229, 3406, 3589
XbaI TCTAGA 2 cut(s) 1847, 2963
XceI RCATGY 4 cut(s) 176, 442, 1426, 3737
XmiI GTMKAC 2 cut(s) 2471, 3433
XmnI GAANNNNTTC 4 cut(s) 886, 1015, 2494, 2913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.