Rw2G007080

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008827
Physical Location & Seq
Forward (+)
6794960 .. 6799645
4686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G007080.1

Sequence Viewer

Length: 3504 bp
ATGCTTCACAGGAGCTTCAAGCCCGCCAAATGCAAAACGGCGTTGAAGCTTGCCGTTTCGAGAATAAAGCTGTTGAAGAATAAGAAAGATGCCCAGGTGAAGCAGATAAAGAGGGAATTGGCTCAATTGCTGGAGAGTGGTCAGTACAGGACTGCAAGGATTCGGGTTGAACATGTGGTCAGGGAAGAGAAGTTGAGGGCAGCCTATGAACTTATCGAGATATATTGCGAACTTGTTGCAGCACGATTGCCTATGGTTGAATCGCAGAAAAACTGCCCAATTGATCTGAAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCGCAAGCACTTCACAGCAAAATACGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGGCCGGATAGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCTGATGGCCCTACCAAGATGAAAATTTTGGCTGCAATTGCAGAGGAACATAATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTCCTGAGGACCTGCTGAATGGACCAAATACGTTTGGAATGGCCAGTATGGATTCTGCTCCACCTCCACCTACTCATGATGTCAGGGGACCTCCTAATGTCCAAGTTCCGCCTAATAGCATGGCCATTCCACGCAGAAATTATGAGTATCACGATGGACCTATGAATTCTAATGAGCAGAATGCAAGATCATCACCTCACTTGGAGGATTCTGATTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTGCCAAATTTCATCCAGAAACGACATCTTCAGAAACCAGAAGCAGTCGAATGGAAATGCATGTATTTCCTGGGGATGAAAGTTCTTCGGGCAGGCAGAATTGGAATATGCAATTTAAGGATGCTGCATCTGCAGCACAGGCAGCTGCTGAATCAGCAGAACTAGCAGGCATGGCAGCCAGAGCAGCTGCAGAACTCTCGAGGCAATATTCATCTGAAATGCACAATAAAAGTAATCAGTCAGCCTCTGTGCCAACTAATTCAAGTTCCATGAACAATGACCATTTGGGGAATAGTCTCCAGACAGCAGATCGATATCCTCAAAAGAAGTCACCTGAACGTCATGACATTTTAGATAAGGCGAGCATGAAGATACAGTCTGGTAATTCTGAGGATATGCATGCAACGGAGATGCAAACTGCTATCAAACCTGAGAATATTCCTTATTTTGGAGATATAAGATCTGAAAAGAAATCTAGCAGGACTTCCTCCCCATCTAATTCAAGTGTTGGTAGCGATCATCAGGAAGACGTTTTGAAAGAGGATGATCATATTAATTACTTTGCAGATCTGGGTACTGGAAAACAATCTATCAGGTCTTCCTCTCCTTCTCATTCGGGACATTTTAGTAACAATCACGAAAATTTCGCTAGTGTTTCTGGTGAGGGTTCTTTTGTTAGTGATGTTAACATCCATCAAAGCACAATTGGAATGAATTCTGAGGGAAATGCTCCTCTGGTTTTTGATGATTCTGGTTCAGACGATGATAACTACAAGGGACAAGAATCAAGTTTGTTTTTTTCTTCTCCTGCTAGAAATTCATTTCCTTCAGCTAGTTTAGATCCTTGGAGCTTTGAGCAAAAGAGAGATGAGGTACCATTGAAGTCCGTTTCACCATTACGTTCTTCATCAGTGCAGCACTCCCTTCCCATTTTTTCTGAAGGTTTGACAGGTTCTACTGTCCCTTCGGAACCAAATGACTTCGTGCCTGTTACTTTTGATGCTTCAGATGGCCCAAGTTCAGACAGTGAGGAGGAGTTGGACACGTCGATGCTTGTCCTGAGCAAAGATTCTAAATTTTCTCATGAGAAGACTGTTCAATCCATAAATTCGGGAAAAAGCCAGAGTGCAAGCCACAAGTCATTAGCATCTTCATCCTCTGATGAGGTACATCCTGAGAGAACCCAGGGAATTGAATATAGTGTTTTCTCAGATAAAAGGTTTGATGATGATGAATTGCCCGGTAGTGAACCATCTCCAAGGCTGAAAAAATCTGGATTAGATACAAGTGCCAAGGATGATTTACCTCAAACAGTGAAAGACAGTGAGGAATGTAGTTGTGTAAGTGATACTGACAATGAGTTGTTGGCCTTTGGGGTCTTGAAAGGCGGCCTTCGGAACAAGGGTTATAGGTATCCACCATATAATCGGAATCCATCAGGCAATGCTTTTTTGGGCAAACAAATTACTCAAGATGGTCCAACAGTCAGGACTTCCGATGTTTCTCATACGCCTATTGATGAGCCATATAGCCGGAAAGGAAGCACTAAGCTAAATAAGGAACAAAGCACTAAAACCCCAGTCACACATGTCACTCCTGATGATGATTCTTCAGAGGATGAACTGTCACATCAAACTCTAAGCAGTAGTCGCCAAGACTTGTACAATCAGAAATCGGTCTCTCGAGTAGTACATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCTTAGCAATAGTCGCCAAGACCTGTCCCATCATAAATCGGTATCCCAAGGAAAGACAAGCTCAACGTCTGCATTTTTCTTTAATTCAGAGGATAGTGAGGCTGAGGAAGATGTTCCTAGAAAGATTTCCACCACCATTGCTCGTCCAAGTTCAAAATTTTCTAGAAGGACACAACCCTCTTCTTCAAATTCTGTTAGAAGTTCTAGCATAAGGACAACAGTTGTTCCTGATGCACCAAGGACTTCAGACTATGGAAAATCCTCTTCAAGAAGTTCCAATGACGCTGAAACTCTACCAGAGCCCTCAAGAAGTCAGGATTGGCAGAGACCTATAGAACAAACAAGTTCTTATTCAATTCCAGAATCCAAGAAGACTGGGACATCAGTCAATGAAAATTCCTCCTCAAGAAGTTCTTATGCCACTGAAACTCTACCAAGTGCCTCATCCCAGACCAAGCGCTCAGACCGTTTGGCAAGTCAGGAGTGGCGTAGACCAATAGAACAAGCAGCACCTAAACCAATACCAGAATCTAAGAGGTCCTCGCGTGTGGAGACTTCAAACTTATTTCCAAGGGAGGAATCATCCAATACTCCAATGACTGTAAGATCAGAGAGAACTGAAGCTACTGAAACTTCAAAGTCCTCGGGTTCAAAGTCATTTGGAAGAGAACAATCATCTAATCCTCCAAAGATTGAAAGATCAGAAAGCAGCGAAAGTTCAAACCAATCGTCCAATCTTCCTGCAAAGGTTGTAAGATCACAGAGAAGTGACCCTTCAAAGTTCTCTGCTCCGAAGTCATTTACAAGGGACCAAACTTCCAATCCTCCTCCAAAGATTGTAAGATCAGAGAGCGCTGAAACTCCGAAGTCCTCTTCTTCAGTGGGGAAGGCTTCTCATGTCCACCCGAAGCTTCCCGACTCTGATGACTTGTTTGCCAAGTTTACAGCCCTCAAGCAGAATCGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1167

Amino Acids

128.18

Weight (kDa)

5.99

Isoelectric Point (pI)

66.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 4.1e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 576
Acc65I GGTACC 1 cut(s) 1678
AccB1I GGYRCC 1 cut(s) 1678
AccI GTMKAC 1 cut(s) 3025
AccII CGCG 1 cut(s) 3079
AciI CCGC 7 cut(s) 24, 332, 358, 415, 436, 665, 2193
AclWI GGATC 3 cut(s) 527, 540, 1640
AcoI YGGCCR 3 cut(s) 416, 597, 678
AfaI GTAC 7 cut(s) 146, 339, 1381, 1680, 1974, 2468, 2496
AfeI AGCGCT 2 cut(s) 2993, 3388
AfiI CCNNNNNNNGG 2 cut(s) 425, 1253
AflIII ACRYGT 3 cut(s) 172, 1848, 2392
AjiI CACGTC 1 cut(s) 1851
AjnI CCWGG 3 cut(s) 93, 874, 1989
AjuI GAANNNNNNNTTGG 2 cut(s) 2836, 2868
Alw26I GTCTC 5 cut(s) 400, 1106, 2488, 2854, 3080
AlwI GGATC 3 cut(s) 527, 540, 1640
AlwNI CAGNNNCTG 2 cut(s) 953, 1052
Ama87I CYCGRG 4 cut(s) 536, 1003, 2487, 3178
Aor51HI AGCGCT 2 cut(s) 2993, 3388
AoxI GGCC 7 cut(s) 416, 475, 597, 678, 1816, 2172, 2194
ArsI GACNNNNNNTTYG 2 cut(s) 3248, 3280
AseI ATTAAT 1 cut(s) 1359
Asp700I GAANNNNTTC 3 cut(s) 1519, 2646, 2659
Asp718I GGTACC 1 cut(s) 1678
AspLEI GCGC 2 cut(s) 2994, 3389
AspS9I GGNCC 9 cut(s) 476, 565, 578, 644, 713, 1817, 2282, 3072, 3343
AsuC2I CCSGG 1 cut(s) 2046
AsuHPI GGTGA 6 cut(s) 109, 459, 741, 1128, 1478, 1689
AvaI CYCGRG 4 cut(s) 536, 1003, 2487, 3178
AvaII GGWCC 7 cut(s) 565, 578, 644, 713, 2282, 3072, 3343
AxyI CCTNAGG 1 cut(s) 561
BalI TGGCCA 2 cut(s) 599, 680
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 1678
BanII GRGCYC 1 cut(s) 2838
BbsI GAAGAC 4 cut(s) 1338, 1395, 1901, 2912
BbvCI CCTCAGC 1 cut(s) 2637
BccI CCATC 9 cut(s) 467, 704, 1306, 1506, 1808, 2065, 2248, 2273, 2571
BceAI ACGGC 2 cut(s) 38, 54
BcgI CGANNNNNNTGC 4 cut(s) 218, 252, 984, 1018
BciT130I CCWGG 3 cut(s) 95, 876, 1991
BciVI GTATCC 2 cut(s) 2229, 2587
BclI TGATCA 1 cut(s) 1351
BcnI CCSGG 1 cut(s) 2046
BcoDI GTCTC 5 cut(s) 400, 1106, 2488, 2854, 3080
BfaI CTAG 9 cut(s) 324, 968, 1281, 1455, 1617, 1638, 2652, 2697, 2739
BfmI CTRYAG 4 cut(s) 399, 936, 993, 2865
BfoI RGCGCY 2 cut(s) 2995, 3390
BfuAI ACCTGC 1 cut(s) 576
BfuI GTATCC 2 cut(s) 2229, 2587
BglII AGATCT 2 cut(s) 1265, 1372
Bme1390I CCNGG 4 cut(s) 95, 876, 1991, 2046
Bme18I GGWCC 7 cut(s) 565, 578, 644, 713, 2282, 3072, 3343
BmeT110I CYCGRG 4 cut(s) 536, 1003, 2487, 3178
BmgBI CACGTC 1 cut(s) 1851
BmgT120I GGNCC 9 cut(s) 476, 565, 578, 644, 713, 1817, 2282, 3072, 3343
BmiI GGNNCC 5 cut(s) 534, 645, 1680, 1776, 3344
BmrFI CCNGG 4 cut(s) 95, 876, 1991, 2046
BmrI ACTGGG 2 cut(s) 2378, 2919
BmsI GCATC 9 cut(s) 79, 326, 916, 941, 1206, 1795, 1845, 1961, 2755
BmuI ACTGGG 2 cut(s) 2378, 2919
BoxI GACNNNNGTC 1 cut(s) 2918
BpiI GAAGAC 4 cut(s) 1338, 1395, 1901, 2912
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 2 cut(s) 152, 1088
Bpu10I CCTNAGC 2 cut(s) 1865, 2637
BpuEI CTTGAG 4 cut(s) 2259, 2824, 2923, 3470
BpuMI CCSGG 1 cut(s) 2046
Bsa29I ATCGAT 1 cut(s) 1117
BsaBI GATNNNNATC 1 cut(s) 1119
BsaI GGTCTC 2 cut(s) 2488, 2854
Bsc4I CCNNNNNNNGG 2 cut(s) 425, 1253
Bse1I ACTGG 4 cut(s) 600, 1387, 2384, 2914
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 2254, 2670
Bse8I GATNNNNATC 1 cut(s) 1119
BseBI CCWGG 3 cut(s) 95, 876, 1991
BseCI ATCGAT 1 cut(s) 1117
BseJI GATNNNNATC 1 cut(s) 1119
BseLI CCNNNNNNNGG 2 cut(s) 425, 1253
BseMI GCAATG 2 cut(s) 2254, 2670
BseMII CTCAG 9 cut(s) 552, 1185, 1227, 1515, 1856, 1971, 2028, 2628, 3009
BseNI ACTGG 4 cut(s) 600, 1387, 2384, 2914
BseRI GAGGAG 5 cut(s) 1527, 1850, 1853, 2926, 3351
BseX3I CGGCCG 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 1739
Bsh1236I CGCG 1 cut(s) 3079
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 7 cut(s) 418, 477, 599, 680, 1818, 2174, 2196
BshNI GGYRCC 1 cut(s) 1678
BshVI ATCGAT 1 cut(s) 1117
BsiEI CGRYCG 1 cut(s) 419
BsiHKCI CYCGRG 4 cut(s) 536, 1003, 2487, 3178
BsiSI CCGG 3 cut(s) 419, 2046, 2338
BslFI GGGAC 7 cut(s) 657, 1437, 1596, 1751, 2545, 2926, 3356
BslI CCNNNNNNNGG 2 cut(s) 425, 1253
BsmAI GTCTC 5 cut(s) 400, 1106, 2488, 2854, 3080
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 7 cut(s) 657, 1437, 1596, 1751, 2545, 2926, 3356
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 7 cut(s) 418, 477, 599, 680, 1818, 2174, 2196
Bso31I GGTCTC 2 cut(s) 2488, 2854
BsoBI CYCGRG 4 cut(s) 536, 1003, 2487, 3178
Bsp1286I GDGCHC 1 cut(s) 2838
Bsp1407I TGTACA 1 cut(s) 2466
BspACI CCGC 7 cut(s) 24, 332, 358, 415, 436, 665, 2193
BspANI GGCC 7 cut(s) 418, 477, 599, 680, 1818, 2174, 2196
BspCNI CTCAG 9 cut(s) 553, 1186, 1228, 1516, 1857, 1972, 2027, 2629, 3008
BspDI ATCGAT 1 cut(s) 1117
BspFNI CGCG 1 cut(s) 3079
BspHI TCATGA 3 cut(s) 631, 1147, 1888
BspLI GGNNCC 5 cut(s) 534, 645, 1680, 1776, 3344
BspMAI CTGCAG 3 cut(s) 403, 940, 997
BspMI ACCTGC 1 cut(s) 576
BspPI GGATC 3 cut(s) 527, 540, 1640
BspT107I GGYRCC 1 cut(s) 1678
BspTNI GGTCTC 2 cut(s) 2488, 2854
BsrDI GCAATG 2 cut(s) 2254, 2670
BsrGI TGTACA 1 cut(s) 2466
BsrI ACTGG 4 cut(s) 600, 1387, 2384, 2914
BssT1I CCWWGG 6 cut(s) 1649, 2063, 2097, 2581, 2771, 3104
Bst2UI CCWGG 3 cut(s) 95, 876, 1991
Bst6I CTCTTC 6 cut(s) 180, 543, 2719, 2803, 3193, 3412
BstAUI TGTACA 1 cut(s) 2466
BstC8I GCNNGC 8 cut(s) 24, 51, 362, 899, 973, 1168, 1206, 1936
BstFNI CGCG 1 cut(s) 3079
BstH2I RGCGCY 2 cut(s) 2995, 3390
BstHHI GCGC 2 cut(s) 2994, 3389
BstMAI GTCTC 5 cut(s) 400, 1106, 2488, 2854, 3080
BstMCI CGRYCG 1 cut(s) 419
BstNI CCWGG 3 cut(s) 95, 876, 1991
BstNSI RCATGY 5 cut(s) 176, 442, 869, 1208, 2396
BstPAI GACNNNNGTC 1 cut(s) 2918
BstSCI CCNGG 4 cut(s) 93, 874, 1989, 2044
BstSFI CTRYAG 4 cut(s) 399, 936, 993, 2865
BstUI CGCG 1 cut(s) 3079
BstV2I GAAGAC 4 cut(s) 1338, 1395, 1901, 2912
BstX2I RGATCY 4 cut(s) 532, 1265, 1372, 1645
BstYI RGATCY 4 cut(s) 532, 1265, 1372, 1645
BstZI CGGCCG 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 1117
Bsu36I CCTNAGG 1 cut(s) 561
BsuI GTATCC 2 cut(s) 2229, 2587
BsuRI GGCC 7 cut(s) 418, 477, 599, 680, 1818, 2174, 2196
BsuTUI ATCGAT 1 cut(s) 1117
BtrI CACGTC 1 cut(s) 1851
BtsIMutI CAGTG 6 cut(s) 1722, 1837, 2124, 2134, 2955, 3420
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 8 cut(s) 24, 51, 362, 899, 973, 1168, 1206, 1936
CaiI CAGNNNCTG 2 cut(s) 953, 1052
CciI TCATGA 3 cut(s) 631, 1147, 1888
CfoI GCGC 2 cut(s) 2994, 3389
Cfr13I GGNCC 9 cut(s) 476, 565, 578, 644, 713, 1817, 2282, 3072, 3343
ClaI ATCGAT 1 cut(s) 1117
CseI GACGC 1 cut(s) 2825
Csp6I GTAC 7 cut(s) 145, 338, 1380, 1679, 1973, 2467, 2495
CspCI CAANNNNNGTGG 2 cut(s) 2653, 2688
CviQI GTAC 7 cut(s) 145, 338, 1380, 1679, 1973, 2467, 2495
EaeI YGGCCR 3 cut(s) 416, 597, 678
EagI CGGCCG 1 cut(s) 416
Eam1104I CTCTTC 6 cut(s) 180, 543, 2719, 2803, 3193, 3412
EarI CTCTTC 6 cut(s) 180, 543, 2719, 2803, 3193, 3412
EciI GGCGGA 1 cut(s) 654
EclXI CGGCCG 1 cut(s) 416
Eco130I CCWWGG 6 cut(s) 1649, 2063, 2097, 2581, 2771, 3104
Eco24I GRGCYC 1 cut(s) 2838
Eco31I GGTCTC 2 cut(s) 2488, 2854
Eco32I GATATC 2 cut(s) 355, 1121
Eco47I GGWCC 7 cut(s) 565, 578, 644, 713, 2282, 3072, 3343
Eco47III AGCGCT 2 cut(s) 2993, 3388
Eco52I CGGCCG 1 cut(s) 416
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 4 cut(s) 536, 1003, 2487, 3178
EcoO109I RGGNCCY 3 cut(s) 565, 644, 3072
EcoRI GAATTC 2 cut(s) 721, 1519
EcoRII CCWGG 3 cut(s) 93, 874, 1989
EcoRV GATATC 2 cut(s) 355, 1121
EcoT14I CCWWGG 6 cut(s) 1649, 2063, 2097, 2581, 2771, 3104
EcoT22I ATGCAT 2 cut(s) 867, 1206
EcoT38I GRGCYC 1 cut(s) 2838
ErhI CCWWGG 6 cut(s) 1649, 2063, 2097, 2581, 2771, 3104
Esp3I CGTCTC 1 cut(s) 400
FalI AAGNNNNNCTT 6 cut(s) 2181, 2213, 2932, 2964, 3292, 3324
FaqI GGGAC 7 cut(s) 657, 1437, 1596, 1751, 2545, 2926, 3356
FauI CCCGC 1 cut(s) 31
FbaI TGATCA 1 cut(s) 1351
FblI GTMKAC 1 cut(s) 3025
FriOI GRGCYC 1 cut(s) 2838
FspBI CTAG 9 cut(s) 324, 968, 1281, 1455, 1617, 1638, 2652, 2697, 2739
GlaI GCGC 2 cut(s) 2993, 3388
GsuI CTGGAG 2 cut(s) 152, 1088
HaeII RGCGCY 2 cut(s) 2995, 3390
HaeIII GGCC 7 cut(s) 418, 477, 599, 680, 1818, 2174, 2196
HapII CCGG 3 cut(s) 419, 2046, 2338
HgaI GACGC 1 cut(s) 2825
HhaI GCGC 2 cut(s) 2994, 3389
Hin6I GCGC 2 cut(s) 2992, 3387
HinP1I GCGC 2 cut(s) 2992, 3387
HincII GTYRAC 1 cut(s) 1492
HindII GTYRAC 1 cut(s) 1492
HindIII AAGCTT 2 cut(s) 47, 3443
HpaI GTTAAC 1 cut(s) 1492
HpaII CCGG 3 cut(s) 419, 2046, 2338
HphI GGTGA 6 cut(s) 109, 459, 741, 1128, 1478, 1689
Hpy166II GTNNAC 5 cut(s) 1492, 2054, 3026, 3436, 3477
Hpy8I GTNNAC 5 cut(s) 1492, 2054, 3026, 3436, 3477
Hpy99I CGWCG 1 cut(s) 1855
HpyCH4IV ACGT 7 cut(s) 336, 587, 1144, 1335, 1705, 1850, 2600
HpySE526I ACGT 7 cut(s) 336, 587, 1144, 1335, 1705, 1850, 2600
HspAI GCGC 2 cut(s) 2992, 3387
KpnI GGTACC 1 cut(s) 1682
Ksp22I TGATCA 1 cut(s) 1351
KspAI GTTAAC 1 cut(s) 1492
LmnI GCTCC 5 cut(s) 12, 619, 1540, 1653, 3328
LweI GCATC 9 cut(s) 79, 326, 916, 941, 1206, 1795, 1845, 1961, 2755
MaeI CTAG 9 cut(s) 324, 968, 1281, 1455, 1617, 1638, 2652, 2697, 2739
MaeII ACGT 7 cut(s) 336, 587, 1144, 1335, 1705, 1850, 2600
MaeIII GTNAC 7 cut(s) 1134, 1433, 1795, 2386, 2395, 2430, 3302
MfeI CAATTG 4 cut(s) 125, 279, 504, 1509
MflI RGATCY 4 cut(s) 532, 1265, 1372, 1645
MhlI GDGCHC 1 cut(s) 2838
MlsI TGGCCA 2 cut(s) 599, 680
MluNI TGGCCA 2 cut(s) 599, 680
MlyI GAGTC 1 cut(s) 3446
MmeI TCCRAC 2 cut(s) 1824, 2309
Mox20I TGGCCA 2 cut(s) 599, 680
Mph1103I ATGCAT 2 cut(s) 867, 1206
MroXI GAANNNNTTC 3 cut(s) 1519, 2646, 2659
MscI TGGCCA 2 cut(s) 599, 680
MseI TTAA 5 cut(s) 443, 921, 1359, 1491, 2616
MslI CAYNNNNRTG 1 cut(s) 1853
Msp20I TGGCCA 2 cut(s) 599, 680
MspA1I CMGCKG 3 cut(s) 404, 950, 992
MspI CCGG 3 cut(s) 419, 2046, 2338
MspR9I CCNGG 4 cut(s) 95, 876, 1991, 2046
MunI CAATTG 4 cut(s) 125, 279, 504, 1509
Mva1269I GAATGC 1 cut(s) 742
MvaI CCWGG 3 cut(s) 95, 876, 1991
MvnI CGCG 1 cut(s) 3079
NciI CCSGG 1 cut(s) 2046
NlaIV GGNNCC 5 cut(s) 534, 645, 1680, 1776, 3344
NmuCI GTSAC 5 cut(s) 1134, 2386, 2395, 2430, 3302
NsiI ATGCAT 2 cut(s) 867, 1206
NspI RCATGY 5 cut(s) 176, 442, 869, 1208, 2396
PaeI GCATGC 1 cut(s) 1208
PaeR7I CTCGAG 2 cut(s) 1003, 2487
PagI TCATGA 3 cut(s) 631, 1147, 1888
PasI CCCWGGG 1 cut(s) 1990
PciI ACATGT 2 cut(s) 172, 2392
PctI GAATGC 1 cut(s) 742
PdmI GAANNNNTTC 3 cut(s) 1519, 2646, 2659
PleI GAGTC 1 cut(s) 3446
PpsI GAGTC 1 cut(s) 3446
PpuMI RGGWCCY 3 cut(s) 565, 644, 3072
PscI ACATGT 2 cut(s) 172, 2392
PshAI GACNNNNGTC 1 cut(s) 2918
PshBI ATTAAT 1 cut(s) 1359
Psp5II RGGWCCY 3 cut(s) 565, 644, 3072
Psp6I CCWGG 3 cut(s) 93, 874, 1989
PspGI CCWGG 3 cut(s) 93, 874, 1989
PspN4I GGNNCC 5 cut(s) 534, 645, 1680, 1776, 3344
PspPI GGNCC 9 cut(s) 476, 565, 578, 644, 713, 1817, 2282, 3072, 3343
PspPPI RGGWCCY 3 cut(s) 565, 644, 3072
PsrI GAACNNNNNNTAC 2 cut(s) 3142, 3174
PstI CTGCAG 3 cut(s) 403, 940, 997
PstNI CAGNNNCTG 2 cut(s) 953, 1052
PsuI RGATCY 4 cut(s) 532, 1265, 1372, 1645
PvuII CAGCTG 3 cut(s) 404, 950, 992
RsaI GTAC 7 cut(s) 146, 339, 1381, 1680, 1974, 2468, 2496
RsaNI GTAC 7 cut(s) 145, 338, 1380, 1679, 1973, 2467, 2495
RseI CAYNNNNRTG 1 cut(s) 1853
SaqAI TTAA 5 cut(s) 443, 921, 1359, 1491, 2616
Sau96I GGNCC 9 cut(s) 476, 565, 578, 644, 713, 1817, 2282, 3072, 3343
SchI GAGTC 1 cut(s) 3446
ScrFI CCNGG 4 cut(s) 95, 876, 1991, 2046
SduI GDGCHC 1 cut(s) 2838
SfaNI GCATC 9 cut(s) 79, 326, 916, 941, 1206, 1795, 1845, 1961, 2755
SfcI CTRYAG 4 cut(s) 399, 936, 993, 2865
Sfr274I CTCGAG 2 cut(s) 1003, 2487
SinI GGWCC 7 cut(s) 565, 578, 644, 713, 2282, 3072, 3343
SlaI CTCGAG 2 cut(s) 1003, 2487
SmiMI CAYNNNNRTG 1 cut(s) 1853
SmlI CTYRAG 6 cut(s) 1003, 2274, 2487, 2839, 2938, 3485
SmoI CTYRAG 6 cut(s) 1003, 2274, 2487, 2839, 2938, 3485
SphI GCATGC 1 cut(s) 1208
SsiI CCGC 7 cut(s) 24, 332, 358, 415, 436, 665, 2193
SspI AATATT 2 cut(s) 1013, 1243
SspMI CTAG 9 cut(s) 324, 968, 1281, 1455, 1617, 1638, 2652, 2697, 2739
StyD4I CCNGG 4 cut(s) 93, 874, 1989, 2044
StyI CCWWGG 6 cut(s) 1649, 2063, 2097, 2581, 2771, 3104
TaiI ACGT 7 cut(s) 339, 590, 1147, 1338, 1708, 1853, 2603
TaqI TCGA 8 cut(s) 59, 216, 853, 1004, 1117, 1853, 2488, 3496
TaqII GACCGA 1 cut(s) 2470
TatI WGTACW 3 cut(s) 144, 2466, 2494
TauI GCSGC 3 cut(s) 418, 438, 2196
Tru1I TTAA 5 cut(s) 443, 921, 1359, 1491, 2616
Tru9I TTAA 5 cut(s) 443, 921, 1359, 1491, 2616
TscAI CASTG 6 cut(s) 1722, 1837, 2124, 2134, 2962, 3420
TseFI GTSAC 5 cut(s) 1134, 2386, 2395, 2430, 3302
Tsp45I GTSAC 5 cut(s) 1134, 2386, 2395, 2430, 3302
TspGWI ACGGA 3 cut(s) 396, 1226, 1681
TspRI CASTG 6 cut(s) 1722, 1837, 2124, 2134, 2962, 3420
VpaK11BI GGWCC 7 cut(s) 565, 578, 644, 713, 2282, 3072, 3343
VspI ATTAAT 1 cut(s) 1359
XbaI TCTAGA 1 cut(s) 2696
XceI RCATGY 5 cut(s) 176, 442, 869, 1208, 2396
XhoI CTCGAG 2 cut(s) 1003, 2487
XmiI GTMKAC 1 cut(s) 3025
XmnI GAANNNNTTC 3 cut(s) 1519, 2646, 2659
XspI CTAG 9 cut(s) 324, 968, 1281, 1455, 1617, 1638, 2652, 2697, 2739
Zsp2I ATGCAT 2 cut(s) 867, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.