pycom02g05790

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008827
Physical Location & Seq
Reverse (-)
3896364 .. 3900477
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05790.1

Sequence Viewer

Length: 2685 bp
ATGCTTCACAGGAGCTTCAAGCCTGCCAAATGCAAAACGGCATTGAAGCTTGCGGTTTCGCGGATAAAGCTATTGAAGAACAAGAAGGATGCACAGGTGAAGCAGATGAGGAGGGAATTGGCCCAGTTGCTTGAGACTGGCCAGGACCGGACTGCGAGAATTCGGGTTGAACATGTGGTCAGAGAAGAGAAGACAAAGGCAGCATTCGAACTTATCGAGATATATTGCGAGCTTATTGCTGCACGCTTGCCTATGATTGAGTCACAAAAAAATTGCCCCATTGATCTGAAGGAAGCAATTACAAGTGTAGTATTTGCATCTCCAAGATGTGCAGACATACCAGAACTCATGGATGTTCGCAAGCATTTCACAGCTAAATATGGAAAGGAGTTTATCTCTAACGCTGTTGAATTGCGCCCAGATTGTGGCGTAAGTCGCATGTTAGTGGAGAAGTTATCTGCCAAATCACCCGATGGCCCTGCGAAGATGAAAATCTTGGCTGCAATAGCAGAGGAACATAACGTCAAATGGGATCCTGAATCACTTGAAGAGAAAGAGTTCAAGCCTCCAGAGGACCTGCTGAATGGGCCAAATACTTTTGGTAGAATTCCTGCGGAACCTTATCTTGGCCCATCTCCACCTAATCATGATGTCAAGGCACCTTCTAATGCTCAGGTTCCACCTCAAAATTATGGGAATTATGATCCACCCTCGAACTTTAACGAGCAGAATGCAAGATCTTCACCTCACTTTCAGGATTCTCCTTCTTCAAATGTTTCTTCCAACAAAGCAACCAGATCTGGCTCATTTCATTCAGAAGTGAGATCTTCAGGAAATCAAAATGATGGGATGGAAAATAGGCATTTGTCTCCTGAGGATGGAAGCTCTTCTTCACCGGGTAGGCAGAGCTGGAACATGCATTTTAAGGATGCTGCTTCTGCAGCACAGGCAGCTGCTGAATCAGCAGAAATGGCAAGTATGGCTGCCAGAGCAGCGGCTGAACTTTCAAGAAAACATTCTTCAGAATCACGGGATAAGTTTAGCCAGTCAGCTTCCTTAGCTTCTAGTCGAACTTCCACAAATGCTCCTTCTGCGAGTAGTCACCAAATGCAAGATCGATATCCTCACCAAATGGCAGACCGAGATTCTCAAAATAAGTCATCTGAGCCCGAGAAACGTGATTTCACAGATGAAGTGCGTACAAAGAGACAGTCTAGCAACACTAATGTGCATGCAAGTGAAATGCAAAGTGGAGTGAAACCAGATAATATTTCGCATTTTGGCAATGTGAGATCTGAAGAGAAATCTAATTGGCCGTCCTCCCAGTCTAATTCTATGGGAGAAGTGAGTATGAAGAGACAGTCAAGCAACACTGATGTGACGCATGGGAGTGAGATGCAAACTGGGAACACACCTGATAACGTGTCTTATTTTGGTGGTATGAGATCTGAAGAAAAATCTAGTAGGCCTTCCTCCCGATCTAATTCAAGCTTTGGTAGTGAAGATCAAGAAGATGTTTTAAGAGGGAACTTGGAAGATATGAGGACTGGAGAACAATCTATGAGGTCCTCCTCTCATTCTCATTCAAGAAATTTTAGTAATGACCATGAAAATGTTTCAAATGGCTCTGTTGAGGAGTCTTTTGTTAACGATATTAACATCTTCCAAAGCACAAAGGAAACAAATTCTTATGGAAATGCTGCTTTGGTTTTTGATGACTCTGGTTCGGACGATGATAAATGTAAATTTGAGGTGGAGGACAACAAGGGGCAAGAATCTAGTTTGTTTTTTCCTTCGCCTGATAGAAATTCATTCTCTCCTTCAGCAGATTTAGATCCTTGGAGCTCTAGACAACAGACGGATGAAGTACAATTTAAGTACAGTTCACAATTAAGTTCTTCATCGGTGAAGCACTTGCCTCCTGTTTTTTCAGAAAATTTGGCAGGTTCCATGGACTCTCCACAACCAACTGATTTGTTGCCTGTTGCTTTTGATGCTTCAGATGGCCCAAGTTCAGACAGTGAAGAGGAGTTAGACAAGCTTGCCCAGAGCACAGCTTCTAAAATTTCTCCTATGCAGAGCCAAAGTGCAAGTCATAGGTCATTAGGATCTTCATCCTCGGAGGAGATAAATGTGGGTTCTAACAGAAAGCCAGGGTTACTGCCTTCTTTTCGTAATTTGGAATCTGCTGAAGTGCCTCCTGAGAGAAGCCAAAGAGTCGAAAATAGAACGGTCCAGGAGGGAAACTATGATTTTGATGAATTGCCGACTGGTCAGCCACCTGTAACGCCTTGGAAATCTGGATTAGATTCAAATGCCAGGGACGATGTTCAAGCATCAAATTTGCGTCAAACTATGAAGGATAGTGAGATTTCAGAGGGATGTAGTTGTGTAGAAGACACTGACAATGAGCTGAGCTTAGGGATGTTGACAGGTGGGCTGCGAAATAAGGGTTTAAGGCATCCGCCGTATACAAAGAAACTATCAGGAAACTCTTTGTCTGTCAAACAAGATACTGGAGATACCAAGGTTGAGCAACCTTCCTTCTTTCGGTCGGTCAGGACTTCTGTTGTCTCTGAAAGGGAGCAAGGGAGCAAAAGACTGATCAAGGAACGGAGCACACGAACCCCCGTCTCATATGTGGCTTCTGATGATGATTCTTCAGAGGATGAATTTGTCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

895

Amino Acids

98.57

Weight (kDa)

5.46

Isoelectric Point (pI)

64.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 2.4e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 585, 1934
AccB1I GGYRCC 1 cut(s) 658
AccB7I CCANNNNNTGG 1 cut(s) 425
AccI GTMKAC 1 cut(s) 2471
AccII CGCG 1 cut(s) 61
AciI CCGC 5 cut(s) 53, 61, 614, 995, 2465
AclWI GGATC 5 cut(s) 527, 540, 698, 1829, 2116
AcoI YGGCCR 2 cut(s) 139, 1313
AcuI CTGAAG 9 cut(s) 308, 813, 1005, 1317, 1470, 1806, 1983, 2211, 2646
AfaI GTAC 3 cut(s) 1201, 1869, 1880
AfiI CCNNNNNNNGG 4 cut(s) 425, 626, 878, 2550
AflIII ACRYGT 2 cut(s) 172, 1422
AjnI CCWGG 4 cut(s) 141, 2152, 2235, 2318
AjuI GAANNNNNNNTTGG 2 cut(s) 609, 641
AleI CACNNNNGTG 2 cut(s) 1226, 1376
Alw21I GWGCWC 3 cut(s) 1847, 2054, 2621
Alw26I GTCTC 6 cut(s) 128, 873, 1201, 1351, 2578, 2638
AlwI GGATC 5 cut(s) 527, 540, 698, 1829, 2116
AlwNI CAGNNNCTG 2 cut(s) 956, 998
Ama87I CYCGRG 1 cut(s) 1169
AoxI GGCC 8 cut(s) 120, 139, 475, 587, 628, 1313, 1466, 2005
ArsI GACNNNNNNTTYG 4 cut(s) 1196, 1228, 2077, 2109
Asp700I GAANNNNTTC 3 cut(s) 557, 886, 1015
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 9 cut(s) 121, 145, 476, 574, 587, 629, 1566, 2006, 2233
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 7 cut(s) 109, 459, 735, 885, 1094, 1118, 1918
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 1169
AvaII GGWCC 4 cut(s) 145, 574, 1566, 2233
AxyI CCTNAGG 1 cut(s) 873
BaeI ACNNNNGTAYC 2 cut(s) 2514, 2547
BalI TGGCCA 1 cut(s) 141
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 658
BanII GRGCYC 2 cut(s) 1170, 1847
BbsI GAAGAC 2 cut(s) 197, 2403
Bbv12I GWGCWC 3 cut(s) 1847, 2054, 2621
BccI CCATC 6 cut(s) 467, 640, 839, 844, 872, 1997
BceAI ACGGC 3 cut(s) 54, 1300, 2452
BcgI CGANNNNNNTGC 6 cut(s) 218, 252, 461, 495, 713, 747
BciT130I CCWGG 4 cut(s) 143, 2154, 2237, 2320
BclI TGATCA 1 cut(s) 2604
BcnI CCSGG 1 cut(s) 897
BcoDI GTCTC 6 cut(s) 128, 873, 1201, 1351, 2578, 2638
BfaI CTAG 5 cut(s) 1065, 1215, 1461, 1779, 1848
BfmI CTRYAG 1 cut(s) 939
BfuAI ACCTGC 2 cut(s) 585, 1934
BglII AGATCT 5 cut(s) 737, 797, 824, 1292, 1445
BlpI GCTNAGC 1 cut(s) 2414
Bme1390I CCNGG 5 cut(s) 143, 897, 2154, 2237, 2320
Bme18I GGWCC 4 cut(s) 145, 574, 1566, 2233
BmeT110I CYCGRG 1 cut(s) 1169
BmgT120I GGNCC 9 cut(s) 121, 145, 476, 574, 587, 629, 1566, 2006, 2233
BmiI GGNNCC 5 cut(s) 534, 618, 660, 678, 1948
BmrFI CCNGG 5 cut(s) 143, 897, 2154, 2237, 2320
BmrI ACTGGG 3 cut(s) 118, 1318, 1413
BmsI GCATC 7 cut(s) 79, 326, 919, 1386, 1984, 2345, 2470
BmuI ACTGGG 3 cut(s) 118, 1318, 1413
BpiI GAAGAC 2 cut(s) 197, 2403
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 3 cut(s) 552, 1569, 2538
Bpu10I CCTNAGC 3 cut(s) 672, 1057, 2419
Bpu1102I GCTNAGC 1 cut(s) 2414
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 897
Bsa29I ATCGAT 1 cut(s) 1117
BsaBI GATNNNNATC 4 cut(s) 491, 1119, 1833, 2113
BsaJI CCNNGG 7 cut(s) 1838, 1950, 2118, 2153, 2291, 2319, 2526
BsaWI WCCGGW 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 626, 878, 2550
Bse1I ACTGG 8 cut(s) 124, 142, 1045, 1324, 1408, 1552, 2275, 2521
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 1291
Bse8I GATNNNNATC 4 cut(s) 491, 1119, 1833, 2113
BseBI CCWGG 4 cut(s) 143, 2154, 2237, 2320
BseCI ATCGAT 1 cut(s) 1117
BseDI CCNNGG 7 cut(s) 1838, 1950, 2118, 2153, 2291, 2319, 2526
BseJI GATNNNNATC 4 cut(s) 491, 1119, 1833, 2113
BseLI CCNNNNNNNGG 4 cut(s) 425, 626, 878, 2550
BseMI GCAATG 1 cut(s) 1291
BseMII CTCAG 5 cut(s) 686, 864, 1155, 2193, 2405
BseNI ACTGG 8 cut(s) 124, 142, 1045, 1324, 1408, 1552, 2275, 2521
BseRI GAGGAG 5 cut(s) 124, 1561, 1649, 2042, 2138
BsgI GTGCAG 2 cut(s) 225, 351
Bsh1236I CGCG 1 cut(s) 61
Bsh1285I CGRYCG 1 cut(s) 2555
BshFI GGCC 8 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007
BshNI GGYRCC 1 cut(s) 658
BshVI ATCGAT 1 cut(s) 1117
BsiEI CGRYCG 1 cut(s) 2555
BsiHKAI GWGCWC 3 cut(s) 1847, 2054, 2621
BsiHKCI CYCGRG 1 cut(s) 1169
BsiSI CCGG 2 cut(s) 148, 896
BslFI GGGAC 1 cut(s) 2336
BslI CCNNNNNNNGG 4 cut(s) 425, 626, 878, 2550
BsmAI GTCTC 6 cut(s) 128, 873, 1201, 1351, 2578, 2638
BsmBI CGTCTC 1 cut(s) 2638
BsmFI GGGAC 1 cut(s) 2336
BsmI GAATGC 2 cut(s) 203, 736
BsnI GGCC 8 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007
BsoBI CYCGRG 1 cut(s) 1169
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 4 cut(s) 1170, 1847, 2054, 2621
Bsp1720I GCTNAGC 1 cut(s) 2414
Bsp19I CCATGG 1 cut(s) 1950
BspACI CCGC 5 cut(s) 53, 61, 614, 995, 2465
BspANI GGCC 8 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007
BspCNI CTCAG 5 cut(s) 685, 865, 1156, 2194, 2406
BspDI ATCGAT 1 cut(s) 1117
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 5 cut(s) 534, 618, 660, 678, 1948
BspMAI CTGCAG 1 cut(s) 943
BspMI ACCTGC 2 cut(s) 585, 1934
BspPI GGATC 5 cut(s) 527, 540, 698, 1829, 2116
BspQI GCTCTTC 1 cut(s) 892
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 658
BsrDI GCAATG 1 cut(s) 1291
BsrI ACTGG 8 cut(s) 124, 142, 1045, 1324, 1408, 1552, 2275, 2521
BssECI CCNNGG 7 cut(s) 1838, 1950, 2118, 2153, 2291, 2319, 2526
BssNAI GTATAC 1 cut(s) 2472
BssT1I CCWWGG 4 cut(s) 1838, 1950, 2291, 2526
Bst1107I GTATAC 1 cut(s) 2472
Bst2UI CCWGG 4 cut(s) 143, 2154, 2237, 2320
Bst4CI ACNGT 5 cut(s) 1212, 1362, 1883, 2021, 2233
Bst6I CTCTTC 6 cut(s) 180, 543, 892, 1293, 1349, 2019
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 8 cut(s) 24, 51, 230, 244, 248, 362, 1233, 2043
BstDEI CTNAG 7 cut(s) 672, 873, 1057, 1164, 2202, 2414, 2419
BstDSI CCRYGG 1 cut(s) 1950
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 1 cut(s) 417
BstMAI GTCTC 6 cut(s) 128, 873, 1201, 1351, 2578, 2638
BstMCI CGRYCG 1 cut(s) 2555
BstNI CCWGG 4 cut(s) 143, 2154, 2237, 2320
BstNSI RCATGY 4 cut(s) 176, 442, 919, 1235
BstSCI CCNGG 5 cut(s) 141, 895, 2152, 2235, 2318
BstSFI CTRYAG 1 cut(s) 939
BstUI CGCG 1 cut(s) 61
BstV2I GAAGAC 2 cut(s) 197, 2403
BstX2I RGATCY 8 cut(s) 532, 737, 797, 824, 1292, 1445, 1834, 2108
BstYI RGATCY 8 cut(s) 532, 737, 797, 824, 1292, 1445, 1834, 2108
BstZ17I GTATAC 1 cut(s) 2472
Bsu15I ATCGAT 1 cut(s) 1117
Bsu36I CCTNAGG 1 cut(s) 873
BsuRI GGCC 8 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007
BsuTUI ATCGAT 1 cut(s) 1117
BtgI CCRYGG 1 cut(s) 1950
BtsIMutI CAGTG 3 cut(s) 1371, 2026, 2400
BveI ACCTGC 2 cut(s) 585, 1934
Cac8I GCNNGC 8 cut(s) 24, 51, 230, 244, 248, 362, 1233, 2043
CaiI CAGNNNCTG 2 cut(s) 956, 998
CciI TCATGA 1 cut(s) 646
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 9 cut(s) 121, 145, 476, 574, 587, 629, 1566, 2006, 2233
ClaI ATCGAT 1 cut(s) 1117
CseI GACGC 2 cut(s) 1390, 2336
Csp6I GTAC 3 cut(s) 1200, 1868, 1879
CviAII CATG 9 cut(s) 173, 349, 439, 647, 916, 1232, 1385, 1607, 1951
CviQI GTAC 3 cut(s) 1200, 1868, 1879
DdeI CTNAG 7 cut(s) 672, 873, 1057, 1164, 2202, 2414, 2419
EaeI YGGCCR 2 cut(s) 139, 1313
Eam1104I CTCTTC 6 cut(s) 180, 543, 892, 1293, 1349, 2019
EarI CTCTTC 6 cut(s) 180, 543, 892, 1293, 1349, 2019
EciI GGCGGA 1 cut(s) 2454
Ecl136II GAGCTC 1 cut(s) 1845
Eco130I CCWWGG 4 cut(s) 1838, 1950, 2291, 2526
Eco147I AGGCCT 1 cut(s) 1468
Eco24I GRGCYC 2 cut(s) 1170, 1847
Eco32I GATATC 1 cut(s) 1121
Eco47I GGWCC 4 cut(s) 145, 574, 1566, 2233
Eco53kI GAGCTC 1 cut(s) 1845
Eco57I CTGAAG 9 cut(s) 308, 813, 1005, 1317, 1470, 1806, 1983, 2211, 2646
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 1169
EcoICRI GAGCTC 1 cut(s) 1845
EcoO109I RGGNCCY 2 cut(s) 574, 1566
EcoRI GAATTC 2 cut(s) 159, 606
EcoRII CCWGG 4 cut(s) 141, 2152, 2235, 2318
EcoRV GATATC 1 cut(s) 1121
EcoT14I CCWWGG 4 cut(s) 1838, 1950, 2291, 2526
EcoT22I ATGCAT 1 cut(s) 921
EcoT38I GRGCYC 2 cut(s) 1170, 1847
ErhI CCWWGG 4 cut(s) 1838, 1950, 2291, 2526
Esp3I CGTCTC 1 cut(s) 2638
FaeI CATG 9 cut(s) 176, 352, 442, 650, 919, 1235, 1388, 1610, 1954
FalI AAGNNNNNCTT 2 cut(s) 874, 906
FaqI GGGAC 1 cut(s) 2336
FatI CATG 9 cut(s) 172, 348, 438, 646, 915, 1231, 1384, 1606, 1950
FauNDI CATATG 1 cut(s) 2638
FbaI TGATCA 1 cut(s) 2604
FblI GTMKAC 1 cut(s) 2471
FriOI GRGCYC 2 cut(s) 1170, 1847
FspBI CTAG 5 cut(s) 1065, 1215, 1461, 1779, 1848
GlaI GCGC 1 cut(s) 416
GsuI CTGGAG 3 cut(s) 552, 1569, 2538
HaeIII GGCC 8 cut(s) 122, 141, 477, 589, 630, 1315, 1468, 2007
HapII CCGG 2 cut(s) 148, 896
HgaI GACGC 2 cut(s) 1390, 2336
HhaI GCGC 1 cut(s) 417
Hin1II CATG 9 cut(s) 176, 352, 442, 650, 919, 1235, 1388, 1610, 1954
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 2 cut(s) 1648, 2430
HindII GTYRAC 2 cut(s) 1648, 2430
HindIII AAGCTT 3 cut(s) 47, 1489, 2039
HpaI GTTAAC 1 cut(s) 1648
HpaII CCGG 2 cut(s) 148, 896
HphI GGTGA 7 cut(s) 109, 459, 735, 885, 1094, 1118, 1918
Hpy166II GTNNAC 4 cut(s) 1648, 1886, 2430, 2472
Hpy8I GTNNAC 4 cut(s) 1648, 1886, 2430, 2472
HpyCH4III ACNGT 5 cut(s) 1212, 1362, 1883, 2021, 2233
HpyCH4IV ACGT 3 cut(s) 522, 1177, 1422
HpyF3I CTNAG 7 cut(s) 672, 873, 1057, 1164, 2202, 2414, 2419
HpySE526I ACGT 3 cut(s) 522, 1177, 1422
Hsp92II CATG 9 cut(s) 176, 352, 442, 650, 919, 1235, 1388, 1610, 1954
HspAI GCGC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 2604
KspAI GTTAAC 1 cut(s) 1648
LguI GCTCTTC 1 cut(s) 892
LmnI GCTCC 6 cut(s) 12, 1090, 1842, 2584, 2592, 2616
LweI GCATC 7 cut(s) 79, 326, 919, 1386, 1984, 2345, 2470
MaeI CTAG 5 cut(s) 1065, 1215, 1461, 1779, 1848
MaeII ACGT 3 cut(s) 522, 1177, 1422
MaeIII GTNAC 6 cut(s) 261, 1100, 1378, 2157, 2284, 2677
MflI RGATCY 8 cut(s) 532, 737, 797, 824, 1292, 1445, 1834, 2108
MhlI GDGCHC 4 cut(s) 1170, 1847, 2054, 2621
MlsI TGGCCA 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 5 cut(s) 269, 1646, 1712, 1949, 2226
MmeI TCCRAC 1 cut(s) 807
Mox20I TGGCCA 1 cut(s) 141
Mph1103I ATGCAT 1 cut(s) 921
MroXI GAANNNNTTC 3 cut(s) 557, 886, 1015
MscI TGGCCA 1 cut(s) 141
MseI TTAA 8 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456
MslI CAYNNNNRTG 6 cut(s) 443, 1226, 1236, 1376, 1389, 1611
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 2 cut(s) 953, 995
MspI CCGG 2 cut(s) 148, 896
MspR9I CCNGG 5 cut(s) 143, 897, 2154, 2237, 2320
Mva1269I GAATGC 2 cut(s) 203, 736
MvaI CCWGG 4 cut(s) 143, 2154, 2237, 2320
MvnI CGCG 1 cut(s) 61
NciI CCSGG 1 cut(s) 897
NcoI CCATGG 1 cut(s) 1950
NdeI CATATG 1 cut(s) 2638
NlaIII CATG 9 cut(s) 176, 352, 442, 650, 919, 1235, 1388, 1610, 1954
NlaIV GGNNCC 5 cut(s) 534, 618, 660, 678, 1948
NmuCI GTSAC 4 cut(s) 261, 1100, 1378, 2677
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 4 cut(s) 176, 442, 919, 1235
NspV TTCGAA 1 cut(s) 207
OliI CACNNNNGTG 2 cut(s) 1226, 1376
PaeI GCATGC 1 cut(s) 1235
PagI TCATGA 1 cut(s) 646
PceI AGGCCT 1 cut(s) 1468
PciI ACATGT 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 892
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 2620
PctI GAATGC 2 cut(s) 203, 736
PdmI GAANNNNTTC 3 cut(s) 557, 886, 1015
PfeI GAWTC 9 cut(s) 539, 758, 959, 1025, 1145, 1775, 2183, 2309, 2657
PflMI CCANNNNNTGG 1 cut(s) 425
PfoI TCCNGGA 1 cut(s) 2235
PleI GAGTC 5 cut(s) 268, 1645, 1712, 1949, 2225
PpsI GAGTC 5 cut(s) 268, 1645, 1712, 1949, 2225
PpuMI RGGWCCY 2 cut(s) 574, 1566
PscI ACATGT 1 cut(s) 172
Psp124BI GAGCTC 1 cut(s) 1847
Psp5II RGGWCCY 2 cut(s) 574, 1566
Psp6I CCWGG 4 cut(s) 141, 2152, 2235, 2318
PspGI CCWGG 4 cut(s) 141, 2152, 2235, 2318
PspN4I GGNNCC 5 cut(s) 534, 618, 660, 678, 1948
PspPI GGNCC 9 cut(s) 121, 145, 476, 574, 587, 629, 1566, 2006, 2233
PspPPI RGGWCCY 2 cut(s) 574, 1566
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 2 cut(s) 956, 998
PsuI RGATCY 8 cut(s) 532, 737, 797, 824, 1292, 1445, 1834, 2108
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 3 cut(s) 1201, 1869, 1880
RsaNI GTAC 3 cut(s) 1200, 1868, 1879
RseI CAYNNNNRTG 6 cut(s) 443, 1226, 1236, 1376, 1389, 1611
SacI GAGCTC 1 cut(s) 1847
SapI GCTCTTC 1 cut(s) 892
SaqAI TTAA 8 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456
Sau96I GGNCC 9 cut(s) 121, 145, 476, 574, 587, 629, 1566, 2006, 2233
SchI GAGTC 5 cut(s) 269, 1646, 1712, 1949, 2226
ScrFI CCNGG 5 cut(s) 143, 897, 2154, 2237, 2320
SduI GDGCHC 4 cut(s) 1170, 1847, 2054, 2621
SfaNI GCATC 7 cut(s) 79, 326, 919, 1386, 1984, 2345, 2470
SfcI CTRYAG 1 cut(s) 939
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 4 cut(s) 145, 574, 1566, 2233
SmiMI CAYNNNNRTG 6 cut(s) 443, 1226, 1236, 1376, 1389, 1611
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 1235
SseBI AGGCCT 1 cut(s) 1468
SsiI CCGC 5 cut(s) 53, 61, 614, 995, 2465
SspI AATATT 1 cut(s) 1270
SspMI CTAG 5 cut(s) 1065, 1215, 1461, 1779, 1848
SstI GAGCTC 1 cut(s) 1847
StuI AGGCCT 1 cut(s) 1468
StyD4I CCNGG 5 cut(s) 141, 895, 2152, 2235, 2318
StyI CCWWGG 4 cut(s) 1838, 1950, 2291, 2526
TaaI ACNGT 5 cut(s) 1212, 1362, 1883, 2021, 2233
TaiI ACGT 3 cut(s) 525, 1180, 1425
TaqI TCGA 6 cut(s) 207, 216, 713, 1069, 1117, 2220
TaqII GACCGA 3 cut(s) 1155, 2541, 2545
TatI WGTACW 2 cut(s) 1867, 1878
TauI GCSGC 1 cut(s) 998
TfiI GAWTC 9 cut(s) 539, 758, 959, 1025, 1145, 1775, 2183, 2309, 2657
Tru1I TTAA 8 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456
Tru9I TTAA 8 cut(s) 720, 924, 1520, 1647, 1656, 1875, 1892, 2456
TscAI CASTG 3 cut(s) 1378, 2026, 2407
TseFI GTSAC 4 cut(s) 261, 1100, 1378, 2677
Tsp45I GTSAC 4 cut(s) 261, 1100, 1378, 2677
TspGWI ACGGA 2 cut(s) 1874, 2629
TspRI CASTG 3 cut(s) 1378, 2026, 2407
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 4 cut(s) 145, 574, 1566, 2233
XbaI TCTAGA 1 cut(s) 1847
XceI RCATGY 4 cut(s) 176, 442, 919, 1235
XmiI GTMKAC 1 cut(s) 2471
XmnI GAANNNNTTC 3 cut(s) 557, 886, 1015
XspI CTAG 5 cut(s) 1065, 1215, 1461, 1779, 1848
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.