Rroxscaffold_2G00148810

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008827
Physical Location & Seq
Forward (+)
86390420 .. 86395474
5055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148810.1

Sequence Viewer

Length: 3651 bp
ATGCTTCACAGGAGCTTCAAGCCCGCCAAATGCAAAACGGCGTTGAAGCTTGCCGTTTCGAGAATCAAGCTGTTGAAGAATAAGAAAGATGCCCAGGTGAAGCAGATAAAGAGGGAATTGGCCCAATTGCTGGAGAGTGGTCAGTACAGGACTGCTAGGATACGGGTTGAACATGTGGTGAGGGAAGAGAAGTTGAGGGCAGCGTATGAACTTATTGAGATATATTGCGAACTTGTTGCAGCACGATTGCCTATGGTTGAATCGCAGAAAAACTGCCCAATTGATCTGAAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATAGATATCCGCAAGCACTTCACAGCAAAATACGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGGCCGGATAGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCTGATGGCCCCACCAAGATGAAAATTTTGGCTGCAATTGCAGAGGAACATAACGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTCCTGAGGACCTGCTGAACGGACCAAATACGTTTGGAATGGCCAGTATGGATTCTGCTCCACCTCCACATACTCATGACGTCAGGGGACCTCCTAATGTCCAAGTTCCGCCTAATAGCATGGCCAGTAAAGCTCATGTGGAACCTCCCTCTGGTCCACCTCCACCTGATCAACCTCCAATTGCTCAAGTTCCACGCAGAAATTATGAGTATCACGATGGACCTATGAATTCTAATGAGCAGAATGCAAGATCATCACCTCACTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTGCCAAATTTCATCCAGAAATGACATCTTCAGAAACCAGAAGCAGTCGAATGGAAATGCATTTATTTCCTGGGGATGAAAGTTCTTCGGGCAGGCAGAATTGGAATATGCAATTTAAGGATGCTGCATCTGCAGCACAGGCAGCTGCTGAATCAGCAGAACTAGCAGGCATGGCAGCCAGAGCAGCTGCAGAACTCTCGAGGCAATATTCATCTGAAATGCACAATAAAAGTAATCCGTCAGCCTCTGTGGCATCTAATTCAAGTTCCATGAACAATGACCATTTGGGGAATAGTCTCCAGACGGCAGATCGATATCCTCAAAGGAAGTCACCTGAACGTCATGACATTTTAGATAAGGCGAGCATGAAGAGACAGTCTGGTAATTCTGAGGATATGCATGCAACTGAGATGCAAACTGCTATCAAACCTGAGAATATTCCTTATTTTGGAGATATAAGATCCGAAAAGAAATCTAGCAGCACTTCCTCCCCATCTAATTCAAGTGTTGGTAGCGATCATCAGGAAGACGTTTTGAAAGAGGATGATCATATTAATTACTTTGCAGATCTGGGTACTGGAAAACAATCTATCAGGTCTTCCTCTCCTTCTCATTCTGGACATTTTAGTAACAATCACGAAAATTTCGCTAGTGTTTCTGGTGAGGGTTCTTTTGTTAGTGATGATAACATCCATCAAAGCACAATTGGAATGAATTCTGAGGGAAATGCTCCTCTGGTTTTTGATGATTCTGGTTCAGACGATGATAACTACAAGGGACAAGAATCAAGTTTGTTTTTTTCTTCTCCTGCTAGAAATTCATTTCCTTCAGCTAGTTTAGATCCTTGGAGCTTTGAGCAAAAGAGAGATGAGGTATTTCCTTCAGCTAGTTTAGATCCTTGGAGCTTTGAGCAAAATAGAGATGAGGTACCATTCAAGTCCGTTTCACCATTACGTTCTTCATCAGTGCAGCACTCCCTTCCCATCTTTTCTGAAGGTTTGATAGGTTCTACAGTCCCTTCGGAACCAAATGACTTTGTGCCTGTTACTTTTGATGCTTCAGATGGCCCAAGTTCAGACAGTGAGGAGGAGTTGGACACGTCGATACTTGTCCTGAGCAAAGATTCTAAATTTCCTCATGAGAAGACTGTTCAATCCATAAATTCGGAAAAGAGCCAGAGTGCAAGCCACAGGTCATTAGCATCTTCATCCTCTGATGAGGTACATCCTGAGAGAACCCAGGGAATTGAATATAGTGTTTTCTCAGATAAAAGGTTTGATGATGATGAATTGCCCGGTAGTGAACCATCTCTGAGGCTGAAAAAATCTGGATTAGATACAAGTGCCAAGGATGATTTACCTCAAACAGTGAAAGGCAGTGAGGAATGTAGTTGTGTAAGTGATACTGACAATGAGTTGTTGGCCTTTGGGGTCTTGAAAGGTGGCCTTCGAAACAAGGGTTATAGGCATCCACCATATAATCGGCATCCATCAGGCAATGCTTTTTTGGGCAAACAAATTACTCAAGATACTCCGACAGTCAGGACTTCCGATGTTTCTCATACGTCTATTGAAGAGCCATATAGCCGGAAAGGGAGCACTAAGCTAAATAAGGAACAAAGCACTAAAACCCCAGTCATACATGTCACTCCTGATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCAAAGCAGTAGTCGCCAAGACCTGTTCAATCAGAAATCGGTCTCTCGAGTAGTACATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCTTAGCAATAGTCGCCAAGACCTGTCCCATCATAAATCGGTATCCCAAGGAAAGACAAGCTCAACGTCTGCATTTTTCTTTAATTCAGATGATAGTGAGGCTGAGGAAGATGTTCCTAGAAAGATTTCCACCACCATTGCTCGTCCAAGTTCAAAATTTTCTAGAAGGACACAAACCTCTTCTTCAAATTCTGTTAGAAGTTCTAGCATAAGGACAACAGTTGTTCCTGATGCACCAAGGATTTCAGACTATGGAAAATCCTCTTCAAGAAGTTCCAATGACGCTGAAACTCTACCAGAGCCCTCAAGAAGTCAGGATTGGCAGAGACCTATAGAACAAACAAGTTCTTATTCAATTCCAGAATCCAAGAAGACTGGGACATCAGTCAATGGAAATTCCTCCTCAAGAAGTTCTTATGCCACTGAAATTCTGCCAAGTGCCTCATCCCAGACCAAGCGCTCAGACCGTTTGGCAAGTCAGGAGTGGCGTAGACCAGTAGAACAAGCAGCACCTAAACCAATACCAAAATCTAAGAGGTCCTCGCGTGTGGAGACTTCAAACTCATTTCCAAGGGAGGAATCATCCAATACTCCAATGACTGTAAGATCAGAGAGAACTGAAGCTACTGAAACTTCAAAGTCCTCGGGTTCAAAGTCATTTGGAAGAGAACAATCATCTAATCCTCCAAAGATTGAAAGATCAGGAAGCAGCGAAAGTTCAAAGTCCTCGGGTTTTAAGTCATTTGCAAGTGACCAATCGTCCAATCTTCCTGCAAAGGTTGTAAGATCACAGAGAACTGACCCTTCAAAGTTCTCTCCTCCAAAGTCATTTACAAGGGACCAAACTTCCAATCCTCCTCCAAAGATTGTAAGATCAGAGAGCGCTGAAACTCCGAAGTCCTCTTCTTCAGTGGGGAAGGCTTCTCATGTCCACCCGAAGCTTCCCGACTCTGATGACTTGTTTGCCAAGTTTACAGCCCTCAAGCAGAATCGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1216

Amino Acids

133.42

Weight (kDa)

6.07

Isoelectric Point (pI)

66.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 1.2e-59 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 639
Acc36I ACCTGC 1 cut(s) 576
Acc65I GGTACC 1 cut(s) 1795
AccB1I GGYRCC 1 cut(s) 1795
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 1 cut(s) 3142
AccII CGCG 1 cut(s) 3196
AciI CCGC 6 cut(s) 24, 332, 358, 415, 436, 665
AclWI GGATC 5 cut(s) 527, 540, 1323, 1703, 1757
AcoI YGGCCR 3 cut(s) 416, 597, 678
AcyI GRCGYC 1 cut(s) 636
AfaI GTAC 6 cut(s) 146, 339, 1444, 1797, 2091, 2613
AfeI AGCGCT 2 cut(s) 3110, 3535
AfiI CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
AflIII ACRYGT 3 cut(s) 172, 1965, 2509
AhdI GACNNNNNGTC 1 cut(s) 3409
AjiI CACGTC 1 cut(s) 1968
AjnI CCWGG 3 cut(s) 93, 937, 2106
AjuI GAANNNNNNNTTGG 2 cut(s) 2953, 2985
Alw21I GWGCWC 1 cut(s) 2468
Alw26I GTCTC 6 cut(s) 400, 1169, 1234, 2605, 2971, 3197
AlwI GGATC 5 cut(s) 527, 540, 1323, 1703, 1757
AlwNI CAGNNNCTG 2 cut(s) 1016, 1115
Ama87I CYCGRG 5 cut(s) 536, 1066, 2604, 3295, 3379
Aor51HI AGCGCT 2 cut(s) 3110, 3535
AoxI GGCC 8 cut(s) 120, 416, 475, 597, 678, 1933, 2289, 2311
ArsI GACNNNNNNTTYG 2 cut(s) 2554, 2586
AseI ATTAAT 1 cut(s) 1422
Asp700I GAANNNNTTC 3 cut(s) 1582, 2763, 2776
Asp718I GGTACC 1 cut(s) 1795
AspLEI GCGC 2 cut(s) 3111, 3536
AsuC2I CCSGG 1 cut(s) 2163
AsuHPI GGTGA 7 cut(s) 109, 190, 459, 804, 1191, 1541, 1806
AsuII TTCGAA 1 cut(s) 2317
AvaI CYCGRG 5 cut(s) 536, 1066, 2604, 3295, 3379
AvaII GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3189, 3490
AxyI CCTNAGG 1 cut(s) 561
BalI TGGCCA 2 cut(s) 599, 680
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 1795
BanII GRGCYC 1 cut(s) 2955
BbsI GAAGAC 4 cut(s) 1401, 1458, 2018, 3029
Bbv12I GWGCWC 1 cut(s) 2468
BbvCI CCTCAGC 1 cut(s) 2754
BccI CCATC 9 cut(s) 467, 767, 1369, 1569, 1859, 1925, 2182, 2365, 2688
BceAI ACGGC 3 cut(s) 38, 54, 1188
BcgI CGANNNNNNTGC 4 cut(s) 218, 252, 1047, 1081
BciT130I CCWGG 3 cut(s) 95, 939, 2108
BciVI GTATCC 2 cut(s) 153, 2704
BclI TGATCA 2 cut(s) 724, 1414
BcnI CCSGG 1 cut(s) 2163
BcoDI GTCTC 6 cut(s) 400, 1169, 1234, 2605, 2971, 3197
BfmI CTRYAG 5 cut(s) 399, 999, 1056, 1878, 2982
BfoI RGCGCY 2 cut(s) 3112, 3537
BfuAI ACCTGC 1 cut(s) 576
BfuI GTATCC 2 cut(s) 153, 2704
BglI GCCNNNNNGGC 1 cut(s) 1118
BglII AGATCT 1 cut(s) 1435
Bme1390I CCNGG 4 cut(s) 95, 939, 2108, 2163
Bme18I GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3189, 3490
BmeRI GACNNNNNGTC 1 cut(s) 3409
BmeT110I CYCGRG 5 cut(s) 536, 1066, 2604, 3295, 3379
BmgBI CACGTC 1 cut(s) 1968
BmiI GGNNCC 7 cut(s) 478, 534, 645, 699, 1797, 1893, 3491
BmrFI CCNGG 4 cut(s) 95, 939, 2108, 2163
BmrI ACTGGG 2 cut(s) 2495, 3036
BmuI ACTGGG 2 cut(s) 2495, 3036
BoxI GACNNNNGTC 1 cut(s) 3035
BpiI GAAGAC 4 cut(s) 1401, 1458, 2018, 3029
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 2 cut(s) 152, 1151
Bpu10I CCTNAGC 2 cut(s) 1982, 2754
Bpu14I TTCGAA 1 cut(s) 2317
BpuEI CTTGAG 5 cut(s) 726, 2376, 2941, 3040, 3617
BpuMI CCSGG 1 cut(s) 2163
Bsa29I ATCGAT 1 cut(s) 1180
BsaBI GATNNNNATC 1 cut(s) 1182
BsaHI GRCGYC 1 cut(s) 636
BsaI GGTCTC 2 cut(s) 2605, 2971
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
Bse1I ACTGG 6 cut(s) 600, 681, 1450, 2501, 3031, 3146
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 2371, 2787
Bse8I GATNNNNATC 1 cut(s) 1182
BseBI CCWGG 3 cut(s) 95, 939, 2108
BseCI ATCGAT 1 cut(s) 1180
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
BseMI GCAATG 2 cut(s) 2371, 2787
BseNI ACTGG 6 cut(s) 600, 681, 1450, 2501, 3031, 3146
BseRI GAGGAG 6 cut(s) 1590, 1967, 1970, 3043, 3459, 3498
BseX3I CGGCCG 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 1856
Bsh1236I CGCG 1 cut(s) 3196
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1935, 2291, 2313
BshNI GGYRCC 1 cut(s) 1795
BshVI ATCGAT 1 cut(s) 1180
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 2468
BsiHKCI CYCGRG 5 cut(s) 536, 1066, 2604, 3295, 3379
BsiSI CCGG 3 cut(s) 419, 2163, 2455
BslFI GGGAC 6 cut(s) 657, 1659, 1868, 2662, 3043, 3503
BslI CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
BsmAI GTCTC 6 cut(s) 400, 1169, 1234, 2605, 2971, 3197
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 6 cut(s) 657, 1659, 1868, 2662, 3043, 3503
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1935, 2291, 2313
Bso31I GGTCTC 2 cut(s) 2605, 2971
BsoBI CYCGRG 5 cut(s) 536, 1066, 2604, 3295, 3379
Bsp119I TTCGAA 1 cut(s) 2317
Bsp1286I GDGCHC 2 cut(s) 2468, 2955
BspACI CCGC 6 cut(s) 24, 332, 358, 415, 436, 665
BspANI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1935, 2291, 2313
BspDI ATCGAT 1 cut(s) 1180
BspFNI CGCG 1 cut(s) 3196
BspHI TCATGA 3 cut(s) 631, 1210, 2005
BspLI GGNNCC 7 cut(s) 478, 534, 645, 699, 1797, 1893, 3491
BspMAI CTGCAG 3 cut(s) 403, 1003, 1060
BspMI ACCTGC 1 cut(s) 576
BspPI GGATC 5 cut(s) 527, 540, 1323, 1703, 1757
BspQI GCTCTTC 1 cut(s) 2436
BspT104I TTCGAA 1 cut(s) 2317
BspT107I GGYRCC 1 cut(s) 1795
BspTNI GGTCTC 2 cut(s) 2605, 2971
BsrDI GCAATG 2 cut(s) 2371, 2787
BsrI ACTGG 6 cut(s) 600, 681, 1450, 2501, 3031, 3146
BssNI GRCGYC 1 cut(s) 636
BssT1I CCWWGG 6 cut(s) 1712, 1766, 2214, 2698, 2888, 3221
Bst2UI CCWGG 3 cut(s) 95, 939, 2108
Bst4CI ACNGT 9 cut(s) 1245, 1882, 1949, 2017, 2236, 2407, 2872, 3119, 3253
Bst6I CTCTTC 8 cut(s) 180, 543, 1232, 2436, 2836, 2920, 3310, 3559
BstACI GRCGYC 1 cut(s) 636
BstBI TTCGAA 1 cut(s) 2317
BstC8I GCNNGC 8 cut(s) 24, 51, 362, 962, 1036, 1231, 1269, 2053
BstFNI CGCG 1 cut(s) 3196
BstH2I RGCGCY 2 cut(s) 3112, 3537
BstHHI GCGC 2 cut(s) 3111, 3536
BstMAI GTCTC 6 cut(s) 400, 1169, 1234, 2605, 2971, 3197
BstMCI CGRYCG 1 cut(s) 419
BstNI CCWGG 3 cut(s) 95, 939, 2108
BstNSI RCATGY 4 cut(s) 176, 442, 1271, 2513
BstPAI GACNNNNGTC 1 cut(s) 3035
BstSCI CCNGG 4 cut(s) 93, 937, 2106, 2161
BstSFI CTRYAG 5 cut(s) 399, 999, 1056, 1878, 2982
BstUI CGCG 1 cut(s) 3196
BstV2I GAAGAC 4 cut(s) 1401, 1458, 2018, 3029
BstX2I RGATCY 5 cut(s) 532, 1328, 1435, 1708, 1762
BstYI RGATCY 5 cut(s) 532, 1328, 1435, 1708, 1762
BstZI CGGCCG 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 1180
Bsu36I CCTNAGG 1 cut(s) 561
BsuI GTATCC 2 cut(s) 153, 2704
BsuRI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1935, 2291, 2313
BsuTUI ATCGAT 1 cut(s) 1180
BtrI CACGTC 1 cut(s) 1968
BtsI GCAGTG 1 cut(s) 2251
BtsIMutI CAGTG 6 cut(s) 1839, 1954, 2241, 2251, 3072, 3567
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 8 cut(s) 24, 51, 362, 962, 1036, 1231, 1269, 2053
CaiI CAGNNNCTG 2 cut(s) 1016, 1115
CciI TCATGA 3 cut(s) 631, 1210, 2005
CfoI GCGC 2 cut(s) 3111, 3536
ClaI ATCGAT 1 cut(s) 1180
CseI GACGC 1 cut(s) 2942
Csp6I GTAC 6 cut(s) 145, 338, 1443, 1796, 2090, 2612
CspCI CAANNNNNGTGG 2 cut(s) 2770, 2805
CviQI GTAC 6 cut(s) 145, 338, 1443, 1796, 2090, 2612
DriI GACNNNNNGTC 1 cut(s) 3409
EaeI YGGCCR 3 cut(s) 416, 597, 678
EagI CGGCCG 1 cut(s) 416
Eam1104I CTCTTC 8 cut(s) 180, 543, 1232, 2436, 2836, 2920, 3310, 3559
Eam1105I GACNNNNNGTC 1 cut(s) 3409
EarI CTCTTC 8 cut(s) 180, 543, 1232, 2436, 2836, 2920, 3310, 3559
EciI GGCGGA 1 cut(s) 654
EclXI CGGCCG 1 cut(s) 416
Eco130I CCWWGG 6 cut(s) 1712, 1766, 2214, 2698, 2888, 3221
Eco24I GRGCYC 1 cut(s) 2955
Eco31I GGTCTC 2 cut(s) 2605, 2971
Eco32I GATATC 2 cut(s) 355, 1184
Eco47I GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3189, 3490
Eco47III AGCGCT 2 cut(s) 3110, 3535
Eco52I CGGCCG 1 cut(s) 416
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 5 cut(s) 536, 1066, 2604, 3295, 3379
EcoO109I RGGNCCY 3 cut(s) 565, 644, 3189
EcoRI GAATTC 2 cut(s) 784, 1582
EcoRII CCWGG 3 cut(s) 93, 937, 2106
EcoRV GATATC 2 cut(s) 355, 1184
EcoT14I CCWWGG 6 cut(s) 1712, 1766, 2214, 2698, 2888, 3221
EcoT22I ATGCAT 2 cut(s) 930, 1269
EcoT38I GRGCYC 1 cut(s) 2955
ErhI CCWWGG 6 cut(s) 1712, 1766, 2214, 2698, 2888, 3221
Esp3I CGTCTC 1 cut(s) 400
FalI AAGNNNNNCTT 4 cut(s) 2298, 2330, 3049, 3081
FaqI GGGAC 6 cut(s) 657, 1659, 1868, 2662, 3043, 3503
FauI CCCGC 1 cut(s) 31
FbaI TGATCA 2 cut(s) 724, 1414
FblI GTMKAC 1 cut(s) 3142
FriOI GRGCYC 1 cut(s) 2955
GlaI GCGC 2 cut(s) 3110, 3535
GsuI CTGGAG 2 cut(s) 152, 1151
HaeII RGCGCY 2 cut(s) 3112, 3537
HaeIII GGCC 8 cut(s) 122, 418, 477, 599, 680, 1935, 2291, 2313
HapII CCGG 3 cut(s) 419, 2163, 2455
HgaI GACGC 1 cut(s) 2942
HhaI GCGC 2 cut(s) 3111, 3536
Hin1I GRCGYC 1 cut(s) 636
Hin6I GCGC 2 cut(s) 3109, 3534
HinP1I GCGC 2 cut(s) 3109, 3534
HindIII AAGCTT 2 cut(s) 47, 3590
HpaII CCGG 3 cut(s) 419, 2163, 2455
HphI GGTGA 7 cut(s) 109, 190, 459, 804, 1191, 1541, 1806
Hpy166II GTNNAC 5 cut(s) 713, 2171, 3143, 3583, 3624
Hpy8I GTNNAC 5 cut(s) 713, 2171, 3143, 3583, 3624
Hpy99I CGWCG 1 cut(s) 1972
HpyCH4III ACNGT 9 cut(s) 1245, 1882, 1949, 2017, 2236, 2407, 2872, 3119, 3253
Hsp92I GRCGYC 1 cut(s) 636
HspAI GCGC 2 cut(s) 3109, 3534
KpnI GGTACC 1 cut(s) 1799
Ksp22I TGATCA 2 cut(s) 724, 1414
LguI GCTCTTC 1 cut(s) 2436
LmnI GCTCC 6 cut(s) 12, 619, 1603, 1716, 1770, 2463
MaeIII GTNAC 5 cut(s) 1197, 1496, 1912, 2512, 3401
MfeI CAATTG 5 cut(s) 125, 279, 504, 735, 1572
MflI RGATCY 5 cut(s) 532, 1328, 1435, 1708, 1762
MhlI GDGCHC 2 cut(s) 2468, 2955
MlsI TGGCCA 2 cut(s) 599, 680
MluNI TGGCCA 2 cut(s) 599, 680
MlyI GAGTC 1 cut(s) 3593
MmeI TCCRAC 2 cut(s) 1941, 2426
Mox20I TGGCCA 2 cut(s) 599, 680
Mph1103I ATGCAT 2 cut(s) 930, 1269
MroXI GAANNNNTTC 3 cut(s) 1582, 2763, 2776
MscI TGGCCA 2 cut(s) 599, 680
MseI TTAA 5 cut(s) 443, 984, 1422, 2733, 3387
MslI CAYNNNNRTG 2 cut(s) 485, 630
Msp20I TGGCCA 2 cut(s) 599, 680
MspA1I CMGCKG 3 cut(s) 404, 1013, 1055
MspI CCGG 3 cut(s) 419, 2163, 2455
MspR9I CCNGG 4 cut(s) 95, 939, 2108, 2163
MunI CAATTG 5 cut(s) 125, 279, 504, 735, 1572
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 3 cut(s) 95, 939, 2108
MvnI CGCG 1 cut(s) 3196
NciI CCSGG 1 cut(s) 2163
NlaIV GGNNCC 7 cut(s) 478, 534, 645, 699, 1797, 1893, 3491
NmuCI GTSAC 3 cut(s) 1197, 2512, 3401
NsiI ATGCAT 2 cut(s) 930, 1269
NspI RCATGY 4 cut(s) 176, 442, 1271, 2513
NspV TTCGAA 1 cut(s) 2317
PaeI GCATGC 1 cut(s) 1271
PaeR7I CTCGAG 2 cut(s) 1066, 2604
PagI TCATGA 3 cut(s) 631, 1210, 2005
PasI CCCWGGG 1 cut(s) 2107
PciI ACATGT 2 cut(s) 172, 2509
PciSI GCTCTTC 1 cut(s) 2436
PctI GAATGC 1 cut(s) 805
PdmI GAANNNNTTC 3 cut(s) 1582, 2763, 2776
PflMI CCANNNNNTGG 1 cut(s) 130
PleI GAGTC 1 cut(s) 3593
PpsI GAGTC 1 cut(s) 3593
PpuMI RGGWCCY 3 cut(s) 565, 644, 3189
PscI ACATGT 2 cut(s) 172, 2509
PshAI GACNNNNGTC 1 cut(s) 3035
PshBI ATTAAT 1 cut(s) 1422
Psp5II RGGWCCY 3 cut(s) 565, 644, 3189
Psp6I CCWGG 3 cut(s) 93, 937, 2106
PspGI CCWGG 3 cut(s) 93, 937, 2106
PspN4I GGNNCC 7 cut(s) 478, 534, 645, 699, 1797, 1893, 3491
PspPPI RGGWCCY 3 cut(s) 565, 644, 3189
PsrI GAACNNNNNNTAC 2 cut(s) 3259, 3291
PstI CTGCAG 3 cut(s) 403, 1003, 1060
PstNI CAGNNNCTG 2 cut(s) 1016, 1115
PsuI RGATCY 5 cut(s) 532, 1328, 1435, 1708, 1762
PvuII CAGCTG 3 cut(s) 404, 1013, 1055
RsaI GTAC 6 cut(s) 146, 339, 1444, 1797, 2091, 2613
RsaNI GTAC 6 cut(s) 145, 338, 1443, 1796, 2090, 2612
RseI CAYNNNNRTG 2 cut(s) 485, 630
SapI GCTCTTC 1 cut(s) 2436
SaqAI TTAA 5 cut(s) 443, 984, 1422, 2733, 3387
SchI GAGTC 1 cut(s) 3593
ScrFI CCNGG 4 cut(s) 95, 939, 2108, 2163
SduI GDGCHC 2 cut(s) 2468, 2955
SfcI CTRYAG 5 cut(s) 399, 999, 1056, 1878, 2982
Sfr274I CTCGAG 2 cut(s) 1066, 2604
SfuI TTCGAA 1 cut(s) 2317
SinI GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3189, 3490
SlaI CTCGAG 2 cut(s) 1066, 2604
SmiMI CAYNNNNRTG 2 cut(s) 485, 630
SmlI CTYRAG 7 cut(s) 741, 1066, 2391, 2604, 2956, 3055, 3632
SmoI CTYRAG 7 cut(s) 741, 1066, 2391, 2604, 2956, 3055, 3632
SphI GCATGC 1 cut(s) 1271
SsiI CCGC 6 cut(s) 24, 332, 358, 415, 436, 665
SspI AATATT 2 cut(s) 1076, 1306
StyD4I CCNGG 4 cut(s) 93, 937, 2106, 2161
StyI CCWWGG 6 cut(s) 1712, 1766, 2214, 2698, 2888, 3221
TaaI ACNGT 9 cut(s) 1245, 1882, 1949, 2017, 2236, 2407, 2872, 3119, 3253
TaqI TCGA 8 cut(s) 59, 916, 1067, 1180, 1970, 2317, 2605, 3643
TaqII GACCGA 1 cut(s) 2587
TatI WGTACW 2 cut(s) 144, 2611
TauI GCSGC 2 cut(s) 418, 438
Tru1I TTAA 5 cut(s) 443, 984, 1422, 2733, 3387
Tru9I TTAA 5 cut(s) 443, 984, 1422, 2733, 3387
TscAI CASTG 6 cut(s) 1839, 1954, 2241, 2251, 3079, 3567
TseFI GTSAC 3 cut(s) 1197, 2512, 3401
Tsp45I GTSAC 3 cut(s) 1197, 2512, 3401
TspGWI ACGGA 4 cut(s) 396, 591, 1095, 1798
TspRI CASTG 6 cut(s) 1839, 1954, 2241, 2251, 3079, 3567
Van91I CCANNNNNTGG 1 cut(s) 130
VpaK11BI GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3189, 3490
VspI ATTAAT 1 cut(s) 1422
XbaI TCTAGA 1 cut(s) 2813
XceI RCATGY 4 cut(s) 176, 442, 1271, 2513
XhoI CTCGAG 2 cut(s) 1066, 2604
XmiI GTMKAC 1 cut(s) 3142
XmnI GAANNNNTTC 3 cut(s) 1582, 2763, 2776
ZraI GACGTC 1 cut(s) 637
Zsp2I ATGCAT 2 cut(s) 930, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.