Rh2CG078300

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008827
Physical Location & Seq
Reverse (-)
6408862 .. 6415201
6340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG078300.1

Sequence Viewer

Length: 3192 bp
ATGCCATCGCCGCCTCAACAGGGTGCTCTTTATCCCTTCGATGAGGAATGCTTCATTTGCCTCCAGCCTTTTCGGTTCGGGGAGGTGGTGTCTTTTCCGTGCTCGGAAAACCACCACATGCATGGTAATTGTTACATCTTCGGTTGGGAGGTCCAGCTTTCAGAGAAAGGCCTTGACCAGTCGCATTGTCCTTTGTGCCGACAACAGATTGACTTCGGACGCTCTCACTGCTTCATCCATAAAGAGAGCGGGCTCATACCGGCGGTGACCATACCTTCAAATCCTCCGCCTCCACCGGCGGTTCCTCGCCACGTGTTCCCTCCCCCCGCGGTTCCTCCCCCAGCGGTTCCTCCCCCTCCCCCTCCCCGTCTTCCCCGCCGCGCTCTCGGTATCCCGCGGCCGCCGGCGTCCCCGACGCCCGCCAATGTCGAGAACCACATCCGGCGGTCGCGGGCATCGTGGCGCCAATACGTGGCCGAGCAGCACTACCGGAGGGTTTGGTTTGTGACGATGGCTTTTGCCCCTGACCCGGGCTATCAGGCCCTCAGCAAAGGCTTGGACATTATACAACAGCCATCTGAAGCCGAAGCCGGGCATCAGGAGTCTTTGGAGAAACGTATTGCTGAAGAATGGATGCCTTCTTCACGGAACATGACATATATGCTTGCGCAGTTCAAGCAGAAGGTTTCTATGCTTGATAACTCTGGGAAGCCAGTCCTTGCATCAACTGACAGTTCAAACCCCTGGTGCGCCCTGCTAGAAGAAGTGGTCAGTAAAGCTTACGGAACACTTGGTAAGCCTGAGATTTGTCCTGCTTCAACAGATGCTCACTATTTCCAGCTACAAGGCTTGCCAGCAATTGGATTCTCTCCTATGGCCAACACTCATTTTCTGCTCCATGACCATAACGAGGTCGTGGCTGTTGGAAGATTCCAAAATGGTGGTGCATTCAACGTTATTCCAGATTCTGCTGAACAAAGGGTTGAAGAGAGCTTGATTCGTGTTGGTTTGTTTTATTTTCTTTACAGGAACTGCCTAATTGATCTGGAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCACAAGCATTTCACAGCAAAATATGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGCCCAGATAGTGGTGTAAGCCGCATGGTAATAGAAAAATTATCCACCAAATCACCTGATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTGCTGAGGACCTGCTGAACGGACCAAATACTTTTGGCATGGCTTCTGCTACACCATCACCTACTCATGATGTCAGGGGACCTACTAATGTCCAAGTTCCTCCTAATAGTAGCATGGCCAATAAAGCTCATGTGGAAGCTCCCCCTGGTCCGCTTCCACCTGATAAGCCTCCTAATGCTGAAGTTCCACGCAGAAATTATGAGTTTCAGGATGGACCCGTGAACTCTAATGAGCAGATTGCAAGATCATCACCTCGCTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTACCACATTTCATCCAGAAACGATATCCTCAGGAAGTGATCGAATGGAAATGCATGGGGATGATAATTCTTCGGGCAGGCAGAACTGGAATATGCAATTTAAGGCTAGTTTAGATCCTTGGAGTTTTGAGCAAAAGAGAGATGAGGTACCATTCAAGTCCGTTTCACCATTACGTTCTTCATCAGTGCAGCACTCCCTCCCCATCTTTTCTGAAGGTTTGACAGGTTCTACAGTCCCTTCGGAACCAAATGACTTCGTGCCTGTTACTTTTGATGCTTCAGATGGCCCAAGTTCAGACAGTGAGGAGGAGTTGGACACGTCTATGCTTGTCCTGAGCAAAGATTCTAAATTTTCTCATGAGAAGACTGTTCAATCCATAAATTCGGGAAAGAGCCAGAGTGCAAGCCACAGGTCATTAGCATCTTCATCCTCTGATGAGGTACATCCTGAGAGAACCCCGGGAATTGAATATAGTGTTTTCTCAGATAAAAGGTTTGATGATGATGAATTGCCCGGCAGTGAACCATCTCCGAGGCTGAAAAAATCTGGATTAGATACAAGTGCCAAGGATGATTTACCTCAAACAGTGAAAGACAGTGAGGAATGTAGTTGTGTAAGTGATACTGACAATGAGTTGTTGGCCTTTGGGGTCTTGAAAGGTGGCCTTCGGAACAAGGGTTATAGGCATCCACCATATAATCGGAATCCATCAGGCAATGCTTTTTTGGGCAAACAAATTACTCAAGATACTCCAACAGTCAGGACTTCCGATGTTTCTCATACGTCTATTGAAGAGCCATATAGCCGGAAAGGGAGCACTAAGCTAAATAAGGAACAAAGCACTAAAACCCCAGTCACACATGTCACTGCTGATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCTAAGCAGTAGTCGCCAAGACCTGTACAATCAGAAATCGGTCTCTCGAGTAGTACATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCTTAGCAATAGTCGCCAAGACCTGTCCCATCATAAATCGGTATCCCAAGGAAAGACGAGCTCAACGTCTGCATTTTTCTTTAATTCAGAGGATAGTGAGGCTGAGGAAGATGTTCCTATAAAGATTTCCACCACCATTGCTCGTCCAAGTTCAAAATTTTCTAGAAGGACACAACCCTCTTCTTCAAATTCTGTTAGAAGTTCTAGCATAAGGACAACAGTTGTTCCTGATGCACCAAGGACTTCAGACTATGGAAAATCCTCTTCAAGAAGTTCCAAAGACGCTGAAACTCTACCAGAGCCCTCAAGAAGTCAGGATTGGCAGAGACCTATAGAACAAACAAGTTCTTATTCAATTCCAGAATCCAAGAAGACTGGGACATCAGTCAATGGAAATTCCTCCTCAAGAAGTTCTTATTCCACTGAAACTCCACCAAGTGCCTCATCCCAGACCAAGCGCTCAGAACGTTTGGCAAGTCAGGAGTGGCGTAGACCAGTAGAACAAGCAGCACCTAAACCAATACCAGAATCTAAGAGGTCCTCGCGTGTGGAGATTTCAAACTCATTTGCAAGGGAGGAATCATCCAATACTACAATGACTGTAAGATCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1063

Amino Acids

117.15

Weight (kDa)

5.62

Isoelectric Point (pI)

69.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 343 - 413 4.6e-20 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 1287
Acc65I GGTACC 1 cut(s) 1720
AccB1I GGYRCC 2 cut(s) 462, 1720
AccB7I CCANNNNNTGG 3 cut(s) 472, 860, 1184
AccBSI CCGCTC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 3067
AccII CGCG 5 cut(s) 329, 381, 397, 451, 3121
AclI AACGTT 2 cut(s) 954, 3043
AclWI GGATC 3 cut(s) 1238, 1251, 1682
AcoI YGGCCR 4 cut(s) 398, 474, 876, 1383
AcsI RAATTY 5 cut(s) 1922, 1954, 2732, 2764, 2971
AcuI CTGAAG 8 cut(s) 600, 645, 1467, 1806, 1836, 2442, 2535, 2805
AcvI CACGTG 1 cut(s) 313
AcyI GRCGYC 3 cut(s) 407, 416, 463
AdeI CACNNNGTG 1 cut(s) 3014
AfaI GTAC 5 cut(s) 1098, 1722, 2016, 2510, 2538
AfeI AGCGCT 1 cut(s) 3035
AfiI CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
AflIII ACRYGT 3 cut(s) 312, 1890, 2434
AjiI CACGTC 1 cut(s) 1893
AjnI CCWGG 2 cut(s) 743, 1411
AjuI GAANNNNNNNTTGG 2 cut(s) 2878, 2910
AluBI AGCT 9 cut(s) 157, 779, 841, 993, 1163, 1394, 1406, 2398, 2637
AluI AGCT 9 cut(s) 157, 779, 841, 993, 1163, 1394, 1406, 2398, 2637
Alw21I GWGCWC 4 cut(s) 28, 104, 2393, 2639
Alw26I GTCTC 3 cut(s) 1159, 2530, 2896
AlwI GGATC 3 cut(s) 1238, 1251, 1682
AlwNI CAGNNNCTG 2 cut(s) 968, 1032
Ama87I CYCGRG 4 cut(s) 529, 1247, 2032, 2529
Aor51HI AGCGCT 1 cut(s) 3035
AoxI GGCC 9 cut(s) 169, 398, 474, 540, 876, 1383, 1858, 2214, 2236
ApeKI GCWGC 4 cut(s) 481, 1160, 1762, 3083
ApoI RAATTY 5 cut(s) 1922, 1954, 2732, 2764, 2971
Asp700I GAANNNNTTC 1 cut(s) 2688
Asp718I GGTACC 1 cut(s) 1720
AspLEI GCGC 6 cut(s) 383, 465, 670, 752, 1176, 3036
AspS9I GGNCC 9 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1859, 3114
AsuC2I CCSGG 6 cut(s) 530, 531, 592, 2033, 2034, 2088
AsuHPI GGTGA 5 cut(s) 277, 1218, 1317, 1509, 1731
AvaI CYCGRG 4 cut(s) 529, 1247, 2032, 2529
AvaII GGWCC 7 cut(s) 151, 1276, 1289, 1346, 1415, 1481, 3114
AxyI CCTNAGG 1 cut(s) 1603
BalI TGGCCA 2 cut(s) 878, 1385
BamHI GGATCC 1 cut(s) 1243
BanI GGYRCC 2 cut(s) 462, 1720
BanII GRGCYC 3 cut(s) 255, 2639, 2880
BbrPI CACGTG 1 cut(s) 313
BbsI GAAGAC 3 cut(s) 362, 1943, 2954
Bbv12I GWGCWC 4 cut(s) 28, 104, 2393, 2639
BbvCI CCTCAGC 3 cut(s) 545, 1272, 2679
BbvI GCAGC 4 cut(s) 493, 1172, 1774, 3095
BciT130I CCWGG 2 cut(s) 745, 1413
BciVI GTATCC 2 cut(s) 401, 2629
BcnI CCSGG 6 cut(s) 530, 531, 592, 2033, 2034, 2088
BcoDI GTCTC 3 cut(s) 1159, 2530, 2896
BfaI CTAG 5 cut(s) 758, 1083, 1680, 2739, 2781
BfmI CTRYAG 3 cut(s) 1158, 1803, 2907
BfoI RGCGCY 2 cut(s) 466, 3037
BfuAI ACCTGC 1 cut(s) 1287
BfuI GTATCC 2 cut(s) 401, 2629
BisI GCNGC 9 cut(s) 11, 379, 398, 401, 482, 1161, 1195, 1763, 3084
BlsI GCNGC 9 cut(s) 12, 380, 399, 402, 483, 1162, 1196, 1764, 3085
Bme1390I CCNGG 8 cut(s) 530, 531, 592, 745, 1413, 2033, 2034, 2088
Bme18I GGWCC 7 cut(s) 151, 1276, 1289, 1346, 1415, 1481, 3114
BmeT110I CYCGRG 4 cut(s) 529, 1247, 2032, 2529
BmgBI CACGTC 1 cut(s) 1893
BmgT120I GGNCC 9 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1859, 3114
BmiI GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1722, 1818
BmrFI CCNGG 8 cut(s) 530, 531, 592, 745, 1413, 2033, 2034, 2088
BmrI ACTGGG 2 cut(s) 2420, 2961
BmuI ACTGGG 2 cut(s) 2420, 2961
BoxI GACNNNNGTC 1 cut(s) 2960
BpiI GAAGAC 3 cut(s) 362, 1943, 2954
BplI GAGNNNNNCTC 2 cut(s) 1139, 1171
BpmI CTGGAG 2 cut(s) 47, 1067
Bpu10I CCTNAGC 4 cut(s) 545, 1272, 1907, 2679
BpuEI CTTGAG 3 cut(s) 2301, 2866, 2965
BpuMI CCSGG 6 cut(s) 530, 531, 592, 2033, 2034, 2088
BsaAI YACGTR 2 cut(s) 313, 472
BsaHI GRCGYC 3 cut(s) 407, 416, 463
BsaI GGTCTC 2 cut(s) 2530, 2896
BsaWI WCCGGW 1 cut(s) 489
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 2989, 3019
Bsc4I CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
Bse118I RCCGGY 3 cut(s) 259, 295, 403
Bse1I ACTGG 6 cut(s) 178, 713, 1664, 2426, 2956, 3071
Bse21I CCTNAGG 1 cut(s) 1603
Bse3DI GCAATG 2 cut(s) 2296, 2712
BseBI CCWGG 2 cut(s) 745, 1413
BseLI CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
BseMI GCAATG 2 cut(s) 2296, 2712
BseMII CTCAG 9 cut(s) 559, 792, 1263, 1617, 1898, 2013, 2070, 2670, 3051
BseNI ACTGG 6 cut(s) 178, 713, 1664, 2426, 2956, 3071
BseRI GAGGAG 3 cut(s) 1892, 1895, 2968
BseX3I CGGCCG 1 cut(s) 398
BseXI GCAGC 4 cut(s) 493, 1172, 1774, 3095
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 1781
Bsh1236I CGCG 5 cut(s) 329, 381, 397, 451, 3121
Bsh1285I CGRYCG 2 cut(s) 401, 449
BshFI GGCC 9 cut(s) 171, 400, 476, 542, 878, 1385, 1860, 2216, 2238
BshNI GGYRCC 2 cut(s) 462, 1720
BsiEI CGRYCG 2 cut(s) 401, 449
BsiHKAI GWGCWC 4 cut(s) 28, 104, 2393, 2639
BsiHKCI CYCGRG 4 cut(s) 529, 1247, 2032, 2529
BslFI GGGAC 5 cut(s) 394, 1359, 1793, 2587, 2968
BslI CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
BsmAI GTCTC 3 cut(s) 1159, 2530, 2896
BsmBI CGTCTC 1 cut(s) 1159
BsmFI GGGAC 5 cut(s) 394, 1359, 1793, 2587, 2968
BsmI GAATGC 2 cut(s) 53, 947
BsnI GGCC 9 cut(s) 171, 400, 476, 542, 878, 1385, 1860, 2216, 2238
Bso31I GGTCTC 2 cut(s) 2530, 2896
BsoBI CYCGRG 4 cut(s) 529, 1247, 2032, 2529
Bsp1286I GDGCHC 6 cut(s) 28, 104, 255, 2393, 2639, 2880
Bsp1407I TGTACA 1 cut(s) 2508
Bsp143I GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
BspANI GGCC 9 cut(s) 171, 400, 476, 542, 878, 1385, 1860, 2216, 2238
BspCNI CTCAG 9 cut(s) 558, 793, 1264, 1616, 1899, 2014, 2069, 2671, 3050
BspFNI CGCG 5 cut(s) 329, 381, 397, 451, 3121
BspHI TCATGA 2 cut(s) 1333, 1930
BspLI GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1722, 1818
BspMAI CTGCAG 1 cut(s) 1162
BspMI ACCTGC 1 cut(s) 1287
BspPI GGATC 3 cut(s) 1238, 1251, 1682
BspQI GCTCTTC 1 cut(s) 2361
BspT107I GGYRCC 2 cut(s) 462, 1720
BspTNI GGTCTC 2 cut(s) 2530, 2896
BsrBI CCGCTC 1 cut(s) 249
BsrDI GCAATG 2 cut(s) 2296, 2712
BsrFI RCCGGY 3 cut(s) 259, 295, 403
BsrGI TGTACA 1 cut(s) 2508
BsrI ACTGG 6 cut(s) 178, 713, 1664, 2426, 2956, 3071
BssAI RCCGGY 3 cut(s) 259, 295, 403
BssMI GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
BssNI GRCGYC 3 cut(s) 407, 416, 463
BssT1I CCWWGG 4 cut(s) 1691, 2139, 2623, 2813
Bst2UI CCWGG 2 cut(s) 745, 1413
Bst4CI ACNGT 9 cut(s) 734, 1807, 1874, 1942, 2161, 2171, 2332, 2797, 3178
Bst6I CTCTTC 5 cut(s) 981, 1254, 2361, 2761, 2845
BstACI GRCGYC 3 cut(s) 407, 416, 463
BstAUI TGTACA 1 cut(s) 2508
BstBAI YACGTR 2 cut(s) 313, 472
BstDSI CCRYGG 2 cut(s) 327, 395
BstEII GGTNACC 1 cut(s) 265
BstFNI CGCG 5 cut(s) 329, 381, 397, 451, 3121
BstH2I RGCGCY 2 cut(s) 466, 3037
BstHHI GCGC 6 cut(s) 383, 465, 670, 752, 1176, 3036
BstKTI GATC 6 cut(s) 1045, 1246, 1514, 1615, 1690, 3185
BstMAI GTCTC 3 cut(s) 1159, 2530, 2896
BstMBI GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
BstMCI CGRYCG 2 cut(s) 401, 449
BstMWI GCNNNNNNNGC 8 cut(s) 10, 57, 228, 676, 1391, 1562, 2496, 2589
BstNI CCWGG 2 cut(s) 745, 1413
BstNSI RCATGY 2 cut(s) 121, 2438
BstPAI GACNNNNGTC 1 cut(s) 2960
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 8 cut(s) 528, 529, 590, 743, 1411, 2031, 2032, 2086
BstSFI CTRYAG 3 cut(s) 1158, 1803, 2907
BstUI CGCG 5 cut(s) 329, 381, 397, 451, 3121
BstV1I GCAGC 4 cut(s) 493, 1172, 1774, 3095
BstV2I GAAGAC 3 cut(s) 362, 1943, 2954
BstX2I RGATCY 2 cut(s) 1243, 1687
BstXI CCANNNNNNTGG 2 cut(s) 122, 941
BstYI RGATCY 2 cut(s) 1243, 1687
BstZI CGGCCG 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 1603
BsuI GTATCC 2 cut(s) 401, 2629
BsuRI GGCC 9 cut(s) 171, 400, 476, 542, 878, 1385, 1860, 2216, 2238
BtgI CCRYGG 2 cut(s) 327, 395
BtrI CACGTC 1 cut(s) 1893
BtsI GCAGTG 3 cut(s) 226, 2098, 2439
BtsIMutI CAGTG 8 cut(s) 226, 1764, 1879, 2098, 2166, 2176, 2439, 2997
BveI ACCTGC 1 cut(s) 1287
CaiI CAGNNNCTG 2 cut(s) 968, 1032
CciI TCATGA 2 cut(s) 1333, 1930
CciNI GCGGCCGC 1 cut(s) 398
CfoI GCGC 6 cut(s) 383, 465, 670, 752, 1176, 3036
Cfr10I RCCGGY 3 cut(s) 259, 295, 403
Cfr13I GGNCC 9 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1859, 3114
Cfr42I CCGCGG 2 cut(s) 330, 398
Cfr9I CCCGGG 2 cut(s) 529, 2032
CseI GACGC 4 cut(s) 228, 396, 424, 2867
Csp6I GTAC 5 cut(s) 1097, 1721, 2015, 2509, 2537
CspCI CAANNNNNGTGG 2 cut(s) 2695, 2730
CviQI GTAC 5 cut(s) 1097, 1721, 2015, 2509, 2537
DinI GGCGCC 1 cut(s) 464
DpnI GATC 6 cut(s) 1044, 1245, 1513, 1614, 1689, 3184
DpnII GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
DraIII CACNNNGTG 1 cut(s) 3014
EaeI YGGCCR 4 cut(s) 398, 474, 876, 1383
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 5 cut(s) 981, 1254, 2361, 2761, 2845
EarI CTCTTC 5 cut(s) 981, 1254, 2361, 2761, 2845
EciI GGCGGA 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 2637
EclXI CGGCCG 1 cut(s) 398
Eco130I CCWWGG 4 cut(s) 1691, 2139, 2623, 2813
Eco147I AGGCCT 1 cut(s) 171
Eco24I GRGCYC 3 cut(s) 255, 2639, 2880
Eco31I GGTCTC 2 cut(s) 2530, 2896
Eco32I GATATC 2 cut(s) 1114, 1599
Eco47I GGWCC 7 cut(s) 151, 1276, 1289, 1346, 1415, 1481, 3114
Eco47III AGCGCT 1 cut(s) 3035
Eco52I CGGCCG 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 2637
Eco57I CTGAAG 8 cut(s) 600, 645, 1467, 1806, 1836, 2442, 2535, 2805
Eco72I CACGTG 1 cut(s) 313
Eco81I CCTNAGG 1 cut(s) 1603
Eco88I CYCGRG 4 cut(s) 529, 1247, 2032, 2529
Eco91I GGTNACC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 2637
EcoO109I RGGNCCY 4 cut(s) 541, 1276, 1346, 3114
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 2 cut(s) 743, 1411
EcoRV GATATC 2 cut(s) 1114, 1599
EcoT14I CCWWGG 4 cut(s) 1691, 2139, 2623, 2813
EcoT22I ATGCAT 2 cut(s) 123, 1629
EcoT38I GRGCYC 3 cut(s) 255, 2639, 2880
EgeI GGCGCC 1 cut(s) 464
EheI GGCGCC 1 cut(s) 464
ErhI CCWWGG 4 cut(s) 1691, 2139, 2623, 2813
Esp3I CGTCTC 1 cut(s) 1159
FalI AAGNNNNNCTT 4 cut(s) 2223, 2255, 2974, 3006
FaqI GGGAC 5 cut(s) 394, 1359, 1793, 2587, 2968
FauI CCCGC 6 cut(s) 242, 334, 383, 402, 427, 444
FblI GTMKAC 1 cut(s) 3067
Fnu4HI GCNGC 9 cut(s) 11, 379, 398, 401, 482, 1161, 1195, 1763, 3084
FriOI GRGCYC 3 cut(s) 255, 2639, 2880
Fsp4HI GCNGC 9 cut(s) 11, 379, 398, 401, 482, 1161, 1195, 1763, 3084
FspBI CTAG 5 cut(s) 758, 1083, 1680, 2739, 2781
FspI TGCGCA 1 cut(s) 669
GlaI GCGC 6 cut(s) 382, 464, 669, 751, 1175, 3035
GluI GCNGC 9 cut(s) 11, 379, 398, 401, 482, 1161, 1195, 1763, 3084
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 2 cut(s) 47, 1067
HaeII RGCGCY 2 cut(s) 466, 3037
HaeIII GGCC 9 cut(s) 171, 400, 476, 542, 878, 1385, 1860, 2216, 2238
HgaI GACGC 4 cut(s) 228, 396, 424, 2867
HhaI GCGC 6 cut(s) 383, 465, 670, 752, 1176, 3036
Hin1I GRCGYC 3 cut(s) 407, 416, 463
Hin6I GCGC 6 cut(s) 381, 463, 668, 750, 1174, 3034
HinP1I GCGC 6 cut(s) 381, 463, 668, 750, 1174, 3034
HindIII AAGCTT 1 cut(s) 777
HphI GGTGA 5 cut(s) 277, 1218, 1317, 1509, 1731
Hpy166II GTNNAC 3 cut(s) 1489, 2096, 3068
Hpy8I GTNNAC 3 cut(s) 1489, 2096, 3068
Hpy99I CGWCG 1 cut(s) 418
HpyAV CCTTC 8 cut(s) 46, 285, 648, 676, 1781, 1821, 2249, 2736
HpyCH4III ACNGT 9 cut(s) 734, 1807, 1874, 1942, 2161, 2171, 2332, 2797, 3178
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 57, 228, 676, 1391, 1562, 2496, 2589
Hsp92I GRCGYC 3 cut(s) 407, 416, 463
HspAI GCGC 6 cut(s) 381, 463, 668, 750, 1174, 3034
KasI GGCGCC 1 cut(s) 462
KpnI GGTACC 1 cut(s) 1724
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
KspI CCGCGG 2 cut(s) 330, 398
Kzo9I GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
LguI GCTCTTC 1 cut(s) 2361
LmnI GCTCC 3 cut(s) 900, 1411, 2388
Lsp1109I GCAGC 4 cut(s) 493, 1172, 1774, 3095
MaeI CTAG 5 cut(s) 758, 1083, 1680, 2739, 2781
MaeIII GTNAC 6 cut(s) 131, 265, 505, 1837, 2428, 2437
MalI GATC 6 cut(s) 1044, 1245, 1513, 1614, 1689, 3184
MbiI CCGCTC 1 cut(s) 249
MboI GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 2 cut(s) 1243, 1687
MhlI GDGCHC 6 cut(s) 28, 104, 255, 2393, 2639, 2880
MlsI TGGCCA 2 cut(s) 878, 1385
MluNI TGGCCA 2 cut(s) 878, 1385
Mly113I GGCGCC 1 cut(s) 463
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 3 cut(s) 904, 1866, 2351
Mox20I TGGCCA 2 cut(s) 878, 1385
Mph1103I ATGCAT 2 cut(s) 123, 1629
MreI CGCCGGCG 1 cut(s) 403
MroNI GCCGGC 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 2688
MscI TGGCCA 2 cut(s) 878, 1385
MseI TTAA 2 cut(s) 1674, 2658
MslI CAYNNNNRTG 3 cut(s) 120, 1632, 1895
Msp20I TGGCCA 2 cut(s) 878, 1385
MspA1I CMGCKG 4 cut(s) 329, 344, 397, 1163
MspR9I CCNGG 8 cut(s) 530, 531, 592, 745, 1413, 2033, 2034, 2088
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 2 cut(s) 53, 947
MvaI CCWGG 2 cut(s) 745, 1413
MvnI CGCG 5 cut(s) 329, 381, 397, 451, 3121
MwoI GCNNNNNNNGC 8 cut(s) 10, 57, 228, 676, 1391, 1562, 2496, 2589
NaeI GCCGGC 1 cut(s) 405
NarI GGCGCC 1 cut(s) 463
NciI CCSGG 6 cut(s) 530, 531, 592, 2033, 2034, 2088
NdeII GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
NgoMIV GCCGGC 1 cut(s) 403
NlaIV GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1722, 1818
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 4 cut(s) 265, 505, 2428, 2437
NotI GCGGCCGC 1 cut(s) 398
NsbI TGCGCA 1 cut(s) 669
NsiI ATGCAT 2 cut(s) 123, 1629
NspI RCATGY 2 cut(s) 121, 2438
PaeR7I CTCGAG 1 cut(s) 2529
PagI TCATGA 2 cut(s) 1333, 1930
PceI AGGCCT 1 cut(s) 171
PciI ACATGT 1 cut(s) 2434
PciSI GCTCTTC 1 cut(s) 2361
PcsI WCGNNNNNNNCGW 2 cut(s) 455, 2639
PctI GAATGC 2 cut(s) 53, 947
PdiI GCCGGC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 2688
PflMI CCANNNNNTGG 3 cut(s) 472, 860, 1184
PkrI GCNGC 9 cut(s) 12, 380, 399, 402, 483, 1162, 1196, 1764, 3085
PleI GAGTC 1 cut(s) 610
PluTI GGCGCC 1 cut(s) 466
PmaCI CACGTG 1 cut(s) 313
PmlI CACGTG 1 cut(s) 313
PpsI GAGTC 1 cut(s) 610
Ppu21I YACGTR 2 cut(s) 313, 472
PpuMI RGGWCCY 3 cut(s) 1276, 1346, 3114
PscI ACATGT 1 cut(s) 2434
PshAI GACNNNNGTC 1 cut(s) 2960
Psp124BI GAGCTC 1 cut(s) 2639
Psp1406I AACGTT 2 cut(s) 954, 3043
Psp5II RGGWCCY 3 cut(s) 1276, 1346, 3114
Psp6I CCWGG 2 cut(s) 743, 1411
PspCI CACGTG 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 2 cut(s) 743, 1411
PspN4I GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1722, 1818
PspPI GGNCC 9 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1859, 3114
PspPPI RGGWCCY 3 cut(s) 1276, 1346, 3114
PstI CTGCAG 1 cut(s) 1162
PstNI CAGNNNCTG 2 cut(s) 968, 1032
PsuI RGATCY 2 cut(s) 1243, 1687
PvuII CAGCTG 1 cut(s) 1163
RsaI GTAC 5 cut(s) 1098, 1722, 2016, 2510, 2538
RsaNI GTAC 5 cut(s) 1097, 1721, 2015, 2509, 2537
RseI CAYNNNNRTG 3 cut(s) 120, 1632, 1895
SacI GAGCTC 1 cut(s) 2639
SacII CCGCGG 2 cut(s) 330, 398
SapI GCTCTTC 1 cut(s) 2361
SaqAI TTAA 2 cut(s) 1674, 2658
SatI GCNGC 9 cut(s) 11, 379, 398, 401, 482, 1161, 1195, 1763, 3084
Sau3AI GATC 6 cut(s) 1042, 1243, 1511, 1612, 1687, 3182
Sau96I GGNCC 9 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1859, 3114
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 8 cut(s) 530, 531, 592, 745, 1413, 2033, 2034, 2088
SduI GDGCHC 6 cut(s) 28, 104, 255, 2393, 2639, 2880
SfcI CTRYAG 3 cut(s) 1158, 1803, 2907
SfoI GGCGCC 1 cut(s) 464
Sfr274I CTCGAG 1 cut(s) 2529
Sfr303I CCGCGG 2 cut(s) 330, 398
SgrAI CRCCGGYG 2 cut(s) 295, 403
SgrBI CCGCGG 2 cut(s) 330, 398
SinI GGWCC 7 cut(s) 151, 1276, 1289, 1346, 1415, 1481, 3114
SlaI CTCGAG 1 cut(s) 2529
SmaI CCCGGG 2 cut(s) 531, 2034
SmiMI CAYNNNNRTG 3 cut(s) 120, 1632, 1895
SmlI CTYRAG 4 cut(s) 2316, 2529, 2881, 2980
SmoI CTYRAG 4 cut(s) 2316, 2529, 2881, 2980
SseBI AGGCCT 1 cut(s) 171
SspDI GGCGCC 1 cut(s) 462
SspMI CTAG 5 cut(s) 758, 1083, 1680, 2739, 2781
SstI GAGCTC 1 cut(s) 2639
StuI AGGCCT 1 cut(s) 171
StyD4I CCNGG 8 cut(s) 528, 529, 590, 743, 1411, 2031, 2032, 2086
StyI CCWWGG 4 cut(s) 1691, 2139, 2623, 2813
TaaI ACNGT 9 cut(s) 734, 1807, 1874, 1942, 2161, 2171, 2332, 2797, 3178
TaqI TCGA 4 cut(s) 39, 429, 1615, 2530
TaqII GACCGA 1 cut(s) 2512
TatI WGTACW 2 cut(s) 2508, 2536
TauI GCSGC 5 cut(s) 13, 381, 400, 403, 1197
Tru1I TTAA 2 cut(s) 1674, 2658
Tru9I TTAA 2 cut(s) 1674, 2658
TscAI CASTG 8 cut(s) 233, 1764, 1879, 2098, 2166, 2176, 2446, 3004
TseFI GTSAC 4 cut(s) 265, 505, 2428, 2437
TseI GCWGC 4 cut(s) 481, 1160, 1762, 3083
Tsp45I GTSAC 4 cut(s) 265, 505, 2428, 2437
TspGWI ACGGA 5 cut(s) 87, 661, 798, 1302, 1723
TspMI CCCGGG 2 cut(s) 529, 2032
TspRI CASTG 8 cut(s) 233, 1764, 1879, 2098, 2166, 2176, 2446, 3004
Van91I CCANNNNNTGG 3 cut(s) 472, 860, 1184
VpaK11BI GGWCC 7 cut(s) 151, 1276, 1289, 1346, 1415, 1481, 3114
XapI RAATTY 5 cut(s) 1922, 1954, 2732, 2764, 2971
XbaI TCTAGA 1 cut(s) 2738
XceI RCATGY 2 cut(s) 121, 2438
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 2529
XmaI CCCGGG 2 cut(s) 529, 2032
XmiI GTMKAC 1 cut(s) 3067
XmnI GAANNNNTTC 1 cut(s) 2688
XspI CTAG 5 cut(s) 758, 1083, 1680, 2739, 2781
Zsp2I ATGCAT 2 cut(s) 123, 1629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.