Rorug02G0029400

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008827
Physical Location & Seq
Reverse (-)
2347250 .. 2348318
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0029400.1

Sequence Viewer

Length: 513 bp
ATGGGGAAAGCCATGAATGTTTTAGTTGCTGTGATAGCATTGGCAGCAGTGTTACATAGAACAGAAGCGGATAGAGAGTACGAATTGGTTTGGGCTATTCCTTCTGGAGGAGCCGGCACGTATGCAGCCTGGTCGGCCGAGCATACCTTAGAAATCAATGTCGATAGTATAACATTTGAGTTTTCATCAGGAGAACAAGACCTGGCTTTAGTGACAAAGGAGGATTTTGATAGTTGTACCACTACAAATCCGATATGGGAAACAACAGATCCAGTTAATGTTGGAATTGTTCAACCCGGCACATTTTATTTCATCTGCACATTTGCCGGCCATTGTGCCAAAGGGCAGAAAATTGCCACTAACTGGACCAATTCTTCGGCTGCGGCACCTGCTCCATGTCCAAGTTCAACAGCAACCACTTACTCCGCTCTGCCACTTAAATTTCTTTCCAAAAGAGTTAACAAGCACGGAAACAAGAAGAAAGTGTCTCTGACCTTGGGGGGTGCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

170

Amino Acids

18.11

Weight (kDa)

6.05

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 39 - 116 2.4e-13 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 397
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 2 cut(s) 385, 503
AccB7I CCANNNNNTGG 1 cut(s) 363
AccBSI CCGCTC 1 cut(s) 428
AciI CCGC 3 cut(s) 68, 383, 426
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 2 cut(s) 135, 328
AcsI RAATTY 1 cut(s) 440
AfaI GTAC 2 cut(s) 80, 238
AfiI CCNNNNNNNGG 2 cut(s) 107, 363
AgsI TTSAA 2 cut(s) 293, 408
AjnI CCWGG 2 cut(s) 128, 201
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 1 cut(s) 263
AoxI GGCC 2 cut(s) 135, 328
ApeKI GCWGC 3 cut(s) 44, 125, 380
ApoI RAATTY 1 cut(s) 440
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
AspS9I GGNCC 1 cut(s) 366
AsuC2I CCSGG 1 cut(s) 297
AvaII GGWCC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 385, 503
BbvI GCAGC 3 cut(s) 56, 137, 367
BcgI CGANNNNNNTGC 2 cut(s) 114, 148
BciT130I CCWGG 2 cut(s) 130, 203
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 397
BglI GCCNNNNNGGC 1 cut(s) 134
BisI GCNGC 4 cut(s) 45, 126, 381, 384
BlsI GCNGC 4 cut(s) 46, 127, 382, 385
Bme1390I CCNGG 3 cut(s) 130, 203, 297
Bme18I GGWCC 1 cut(s) 366
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 3 cut(s) 112, 387, 505
BmrFI CCNGG 3 cut(s) 130, 203, 297
BpmI CTGGAG 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 495
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 363
Bse118I RCCGGY 2 cut(s) 113, 326
Bse1I ACTGG 2 cut(s) 272, 368
BseBI CCWGG 2 cut(s) 130, 203
BseDI CCNNGG 1 cut(s) 495
BseLI CCNNNNNNNGG 2 cut(s) 107, 363
BseNI ACTGG 2 cut(s) 272, 368
BseRI GAGGAG 1 cut(s) 123
BseX3I CGGCCG 1 cut(s) 135
BseXI GCAGC 3 cut(s) 56, 137, 367
BsgI GTGCAG 1 cut(s) 301
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 137, 330
BshNI GGYRCC 2 cut(s) 385, 503
BsiEI CGRYCG 1 cut(s) 138
BsiSI CCGG 3 cut(s) 114, 297, 327
BslI CCNNNNNNNGG 2 cut(s) 107, 363
BsmAI GTCTC 1 cut(s) 492
BsnI GGCC 2 cut(s) 137, 330
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 3 cut(s) 68, 383, 426
BspANI GGCC 2 cut(s) 137, 330
BspLI GGNNCC 3 cut(s) 112, 387, 505
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 263
BspT107I GGYRCC 2 cut(s) 385, 503
BsrBI CCGCTC 1 cut(s) 428
BsrFI RCCGGY 2 cut(s) 113, 326
BsrI ACTGG 2 cut(s) 272, 368
BssAI RCCGGY 2 cut(s) 113, 326
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 1 cut(s) 268
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 2 cut(s) 130, 203
BstBAI YACGTR 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 115, 328
BstDEI CTNAG 1 cut(s) 148
BstENI CCTNNNNNAGG 1 cut(s) 105
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 1 cut(s) 268
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 4 cut(s) 35, 44, 134, 389
BstNI CCWGG 2 cut(s) 130, 203
BstSCI CCNGG 3 cut(s) 128, 201, 295
BstV1I GCAGC 3 cut(s) 56, 137, 367
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BstZI CGGCCG 1 cut(s) 135
BsuRI GGCC 2 cut(s) 137, 330
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 115, 328
Cfr10I RCCGGY 2 cut(s) 113, 326
Cfr13I GGNCC 1 cut(s) 366
Csp6I GTAC 2 cut(s) 79, 237
CviAII CATG 2 cut(s) 13, 396
CviJI RGCY 8 cut(s) 11, 95, 113, 128, 137, 206, 330, 380
CviKI_1 RGCY 8 cut(s) 11, 95, 113, 128, 137, 206, 330, 380
CviQI GTAC 2 cut(s) 79, 237
DdeI CTNAG 1 cut(s) 148
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
EaeI YGGCCR 2 cut(s) 135, 328
EagI CGGCCG 1 cut(s) 135
EclXI CGGCCG 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 366
Eco52I CGGCCG 1 cut(s) 135
EcoNI CCTNNNNNAGG 1 cut(s) 105
EcoRII CCWGG 2 cut(s) 128, 201
EcoT14I CCWWGG 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 2 cut(s) 16, 399
FaiI YATR 9 cut(s) 14, 57, 123, 144, 170, 256, 397, 509, 511
FatI CATG 2 cut(s) 12, 395
Fnu4HI GCNGC 4 cut(s) 45, 126, 381, 384
Fsp4HI GCNGC 4 cut(s) 45, 126, 381, 384
GluI GCNGC 4 cut(s) 45, 126, 381, 384
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 2 cut(s) 137, 330
HapII CCGG 3 cut(s) 114, 297, 327
Hin1II CATG 2 cut(s) 16, 399
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 3 cut(s) 114, 297, 327
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 2 cut(s) 252, 492
Hpy188III TCNNGA 2 cut(s) 105, 189
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 1 cut(s) 111
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 2 cut(s) 125, 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 44, 134, 389
HpyF3I CTNAG 1 cut(s) 148
HpySE526I ACGT 1 cut(s) 119
Hsp92II CATG 2 cut(s) 16, 399
KroI GCCGGC 2 cut(s) 113, 326
KroNI GCCGGC 2 cut(s) 115, 328
KspAI GTTAAC 1 cut(s) 460
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 2 cut(s) 110, 397
Lsp1109I GCAGC 3 cut(s) 56, 137, 367
MaeII ACGT 1 cut(s) 119
MaeIII GTNAC 2 cut(s) 51, 211
MalI GATC 1 cut(s) 270
MbiI CCGCTC 1 cut(s) 428
MboI GATC 1 cut(s) 268
MboII GAAGA 2 cut(s) 366, 490
MflI RGATCY 1 cut(s) 268
MluCI AATT 5 cut(s) 83, 285, 351, 370, 440
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 2 cut(s) 101, 214
MroNI GCCGGC 2 cut(s) 113, 326
MseI TTAA 3 cut(s) 276, 438, 459
MspI CCGG 3 cut(s) 114, 297, 327
MspR9I CCNGG 3 cut(s) 130, 203, 297
MvaI CCWGG 2 cut(s) 130, 203
MwoI GCNNNNNNNGC 4 cut(s) 35, 44, 134, 389
NaeI GCCGGC 2 cut(s) 115, 328
NciI CCSGG 1 cut(s) 297
NdeII GATC 1 cut(s) 268
NgoMIV GCCGGC 2 cut(s) 113, 326
NlaIII CATG 2 cut(s) 16, 399
NlaIV GGNNCC 3 cut(s) 112, 387, 505
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 211
PaqCI CACCTGC 1 cut(s) 397
PdiI GCCGGC 2 cut(s) 115, 328
PflMI CCANNNNNTGG 1 cut(s) 363
PkrI GCNGC 4 cut(s) 46, 127, 382, 385
Ppu21I YACGTR 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 128, 201
PspGI CCWGG 2 cut(s) 128, 201
PspN4I GGNNCC 3 cut(s) 112, 387, 505
PspPI GGNCC 1 cut(s) 366
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 2 cut(s) 80, 238
RsaNI GTAC 2 cut(s) 79, 237
SaqAI TTAA 3 cut(s) 276, 438, 459
SatI GCNGC 4 cut(s) 45, 126, 381, 384
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 1 cut(s) 366
ScrFI CCNGG 3 cut(s) 130, 203, 297
SetI ASST 5 cut(s) 122, 149, 204, 391, 497
SinI GGWCC 1 cut(s) 366
Sse9I AATT 5 cut(s) 83, 285, 351, 370, 440
SsiI CCGC 3 cut(s) 68, 383, 426
StyD4I CCNGG 3 cut(s) 128, 201, 295
StyI CCWWGG 1 cut(s) 495
TaiI ACGT 1 cut(s) 122
TaqI TCGA 1 cut(s) 162
TasI AATT 5 cut(s) 83, 285, 351, 370, 440
TauI GCSGC 1 cut(s) 386
Tru1I TTAA 3 cut(s) 276, 438, 459
Tru9I TTAA 3 cut(s) 276, 438, 459
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 3 cut(s) 44, 125, 380
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 3 cut(s) 29, 174, 301
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 1 cut(s) 54
Van91I CCANNNNNTGG 1 cut(s) 363
VpaK11BI GGWCC 1 cut(s) 366
XagI CCTNNNNNAGG 1 cut(s) 105
XapI RAATTY 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.