pycom15g18020

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008827
Physical Location & Seq
Reverse (-)
12775343 .. 12780194
4852 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g18020.1

Sequence Viewer

Length: 3462 bp
ATGCTTCACAAGAGCTTCAAGCCTGCCAAATGCAAAACGGCATTAAAGCTTGCGGTTTCACGGATAAAGCTATTGAAGAACAAGAAGGATGCACAAGTGAAGCAGATGAGGAGGGAATTGGCCCAGTTGCTTGAGACTGGTCAGGACCGGACTGCTAGAATTCGGGTTGAACATGTGGTCAGAGAAGAGAAGATAAAGGCAGCATTCGAACTTATCGAGATATATTGCGAACTTATTGCTGCACGCTTGCCTATGATTGAGTCGCAAAAAAACTGCCCCATTGATCTGAAGGAAGCAATTGCAAGTGTAGTATTTGCATCTCCAAGATGCGCAGACATACCAGAACTCAAGGATATTCGCAAGCATTTCACATCTAAATATGGGAAGGAGTTTATCTCTAACGCTGTTGAATTGCGCCCGGATTGTGGCGTAAGTCGCATGTTAGTGGAGAAGTTATCTACCAAATCACCCGATGGCCCTGCCAAGATGAAAATCTTGGCTGCAATTGCAGAGGAACATAATGTCAAATGGGATCCTGAATCCTTTGAAGAGAAAGAGTCCAAGCTTCCAGAGGACTTGCTGAATGGGCCAAATACTTTTGCTAGAATTCCTGAGGAACCTCCTCGTGGCCCACCTCCTCCTAATCATGATGTCAAGGCACCTTCTAGTGTTCAGGCTCCACCTCAAAATTATGGGAATTATGGTCCACCCACGAACTTTAACAAGCAGAATGCAAGATCTGCACCTCACTTCCAGGATTCTGCTTCTTCAAATGTTTCTTCCAACAAAGCAACGACATCTGGCTCATTTCATTCAGAAGTGAGATCTTCAGGAAATCAAAGTGATGGGATGGAAAATAGACATTCGTCTCCTGGGGATGGAAATTCTTCTTCACCGGGTAGGCAGAGTTGGAACATGCATTTTAAGGATGCTGCTTCTGCTGCTCAGGCAGCTGCTGAATCAGCAGAAATGGCAGGCATGGCTGCCAGAGCAGCTGCTGAACTTTCAAGAAAACACTCTTCAGAATCATGGGATAAATTTAGCCAGTCAGCTCCCTTGGCATCTAATAGAACATCCACAAATGATCCTTCAGTGAATAGTCACCAAATGCAAGATCGACATCCTGATGAAGTGTCAGATCGATATCCTCAAAAGAAGTCATCTGAACCCGAGAAGCATGACTTTGTAAGTGAAGTGAGTACGACAAGACAGTCCAGCAACACTAACGTGTATGCAAGTGAGGTGCAAACTGGAATGAAACCTGATAAAACTTCTTATTTGGGTAATATGAGATCAGAAGAGAAATCAAGTAGACCTTCCCGATCCAATCCTATAGGTAAATTGAGTGTGAAGAGACAGTCAGGCAACACTGGTGTGGAGTATGGGAGAGAGATGCAAACTGGAAACAAACCTGATAATGTTGCTTATTTTGGTGATATGAGATCTGAAGAGAAATCTAGTTGGCCTTCCTCCCGATCTAATTCAAGCATTGGCAGTGATGATCAGGAAGATGTTTTAAGACGGAGTGACGATATTTCTTATTTGGGAAATAAGAGGGGTGGAGAACAATCTGTCTGGTCTTCCTCTCATTCTCATGCAAGAAATTTTAGTAATGATCATGAAAGTGTTTCAAATGGCCGTGATGAGGATTCTTTTGTTAACGATGTTAATATCTTCGAAAGCACAAAGGAAATAAGTTCTGATGGAAATGCTCCTTTGGTTTTTGATGAATCTGGTTCAGACGATGATAAATGTAAATTTGATGTGGAGGACTACAAGGACCAAGAACCCAGTTTGGTTTTTCCTTCTCCTGATAGAAACACATTTTCTCCTTCAGCTAGTTTGGATCCTTGGAGCTCTAAACAACAGACGTATGAAGTACAATTTAATTCCAGTTCACAACTAAGTTCTTCATCGGGGAAGCACTCCCCTCCTGTTTTTTCAGAAAATTCAACAGGTTCCATGGACTCTTCACAACCAAATGACTGGTTGCCTGTTGCTTTTGATGCGTCAGATGGCCCAAGTTCAGACAGTGAAGAGGAGGTAGACAAGCTTGCCAAGAGCACAGTTTCTAAATTTTCCCCTGTGCAGAGCCAAACTGCAAGTCGTAGGTCATTTGTATCTTCATCCTCGGAGGAGACAAGTGTGGGTTCTAACAGAAAGCCAGGGTTGCTGCTTTCATCCCATAATTGGGACTCAACTGAAGCAGATCCCGAGAGAAGCCAAGGGGTCAAAAATAGAATCGTCCCGGAGGAAAGGTATGATGTTGATGAATTGCCTACTGGTCAGCCATCTGTAAGGCTAAGGAAATCTGGAATAGATTCAAATGCCAGGGACGATTTTCAAACGACACATTTGCGTCAAACTATGAAAGATAGTGAGGTTTCAGATGGATGTAGTTGTGTAAGTGACACTGACAATGAGCTGAGCTTAGGGATGTTGACAGGTGGGCTGCGAAATAAGGGTTTAAGGCATCCACCGTATACGAAGAAACTACCAGGAAACTTATTGTCTGTCAAACAAGATACTGAAGATACCAAGGTTGAGCAACCTTCCTTCTTTCCATCAGTCAGGACTTCTGTTGTCTCTGAAAGGGAGCAAGGGAGCAACAAACTGATAAAGGAACGGAGCACGAGAACCCCCGTCTCATATGTGGCTTCTGATGATGATTCTTCTGAGGATGAATTGTCACACGGAACTGCTAGAAGTAGCCAAGACTCATTCAATAAAAAATTGGGATCCGAAGTAAAGGCAAGCTCCAAATCCACGTTTTCTTTCTTTGATTCAGAGGATAGTGAACCGGAGGAAGATCTTCTTACAAAGATTTCCACCAGCAATGCTCGTCCAAATGCTAGACTGTCTAGAAGAACACAGCCGTCTTCTTCAAATTCTGTTAGAAGCTCTAGCTCAAAGACAACAATTGTGTCTGACGTGTCAGGGACTTCAGAGTATGGAAAACCCTCTTCAAGGAGTTCATATGACACTGAAACTGTACTAAAGCCCTCGTCCCAGATCAAGAGCCCAAAGCAGGAGTGGAATATACCTGTAGAACAAGTTGCTCCTAAACCAGTACGAGAATCCAAGAGGACATCGCCTGTAGAGACTTCAAATTCAAACAGAAACTCCATTTCAAGGAGTTCCGATGCCACTGAAACTTTACCAAAGTCCTTGTCCCAGACGAAGAGCTCAGATCGTCCAGGAAGTCAGGAGTGGCATAAACCGGTAGAACAAGCAGCTCCTAAACCAATGCCAGAGTCCAAGATCGATCCTCACTTGTGGAGACTTCAAAGTCATATGGAAACCCCTCGTCAAGAAGCTCCTATGCCGCTGAAAGTCTACCAAAGCCCTCCTCGCAGACCAGGAGCTCAGAGCGTCCAGGAAGTCAGGAGTGGCATAGACCAGCAGAACAAGCAGCTCCTAAACCAATTCGAGAATCCAAGAGTCCTTCACCTGAAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1154

Amino Acids

127.37

Weight (kDa)

6.22

Isoelectric Point (pI)

66.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 8.1e-61 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1210, 3288
Acc16I TGCGCA 1 cut(s) 331
AccB1I GGYRCC 1 cut(s) 658
AccI GTMKAC 4 cut(s) 1312, 2046, 2483, 3336
AciI CCGC 2 cut(s) 53, 3326
AcoI YGGCCR 1 cut(s) 1636
AcuI CTGAAG 9 cut(s) 308, 813, 1005, 1074, 1467, 1818, 2223, 2550, 2928
AfaI GTAC 4 cut(s) 1201, 1881, 2994, 3072
AfiI CCNNNNNNNGG 9 cut(s) 425, 626, 878, 1645, 2190, 2191, 2967, 3028, 3132
AflIII ACRYGT 3 cut(s) 172, 1227, 2931
AgeI ACCGGT 1 cut(s) 3220
AjiI CACGTC 1 cut(s) 2932
AjnI CCWGG 8 cut(s) 753, 871, 2164, 2330, 2497, 3196, 3358, 3375
AleI CACNNNNGTG 2 cut(s) 1226, 1373
Alw21I GWGCWC 5 cut(s) 1859, 2066, 2633, 3188, 3367
Alw26I GTCTC 8 cut(s) 128, 873, 1348, 2132, 2590, 2650, 3095, 3274
AlwNI CAGNNNCTG 2 cut(s) 956, 998
Ama87I CYCGRG 2 cut(s) 1169, 2213
AoxI GGCC 7 cut(s) 120, 475, 587, 628, 1463, 1636, 2017
ArsI GACNNNNNNTTYG 4 cut(s) 2089, 2121, 3155, 3187
AsiGI ACCGGT 1 cut(s) 3220
Asp700I GAANNNNTTC 3 cut(s) 886, 2320, 2811
AspLEI GCGC 2 cut(s) 332, 417
AspS9I GGNCC 8 cut(s) 121, 145, 476, 587, 629, 704, 1780, 2018
AsuC2I CCSGG 3 cut(s) 419, 897, 2249
AsuHPI GGTGA 5 cut(s) 459, 885, 1094, 1445, 3440
AsuII TTCGAA 2 cut(s) 207, 1677
AvaI CYCGRG 2 cut(s) 1169, 2213
AvaII GGWCC 3 cut(s) 145, 704, 1780
AxyI CCTNAGG 1 cut(s) 612
BaeI ACNNNNGTAYC 2 cut(s) 2526, 2559
BamHI GGATCC 3 cut(s) 532, 1846, 2738
BanI GGYRCC 1 cut(s) 658
BanII GRGCYC 4 cut(s) 1859, 3023, 3188, 3367
BauI CACGAG 2 cut(s) 624, 2632
BbsI GAAGAC 2 cut(s) 1572, 2871
Bbv12I GWGCWC 5 cut(s) 1859, 2066, 2633, 3188, 3367
BccI CCATC 9 cut(s) 467, 839, 844, 872, 1697, 2009, 2299, 2384, 2572
BceAI ACGGC 3 cut(s) 54, 1623, 2860
BcgI CGANNNNNNTGC 6 cut(s) 218, 252, 461, 495, 2338, 2372
BciT130I CCWGG 8 cut(s) 755, 873, 2166, 2332, 2499, 3198, 3360, 3377
BclI TGATCA 2 cut(s) 1501, 1615
BcnI CCSGG 3 cut(s) 419, 897, 2249
BcoDI GTCTC 8 cut(s) 128, 873, 1348, 2132, 2590, 2650, 3095, 3274
BfaI CTAG 9 cut(s) 156, 603, 666, 1458, 1839, 2703, 2853, 2862, 2904
BfmI CTRYAG 3 cut(s) 1332, 3045, 3096
BglII AGATCT 4 cut(s) 737, 824, 1442, 2809
BlpI GCTNAGC 1 cut(s) 2426
Bme18I GGWCC 3 cut(s) 145, 704, 1780
BmeT110I CYCGRG 2 cut(s) 1169, 2213
BmgBI CACGTC 1 cut(s) 2932
BmgT120I GGNCC 8 cut(s) 121, 145, 476, 587, 629, 704, 1780, 2018
BmiI GGNNCC 7 cut(s) 534, 618, 660, 678, 1848, 1960, 2740
BmrI ACTGGG 2 cut(s) 118, 1785
BmsI GCATC 9 cut(s) 79, 317, 326, 919, 1070, 1383, 1996, 2482, 3133
BmuI ACTGGG 2 cut(s) 118, 1785
BoxI GACNNNNGTC 1 cut(s) 865
BpiI GAAGAC 2 cut(s) 1572, 2871
BplI GAGNNNNNCTC 2 cut(s) 380, 412
Bpu10I CCTNAGC 3 cut(s) 945, 2303, 2431
Bpu1102I GCTNAGC 1 cut(s) 2426
Bpu14I TTCGAA 2 cut(s) 207, 1677
BpuEI CTTGAG 2 cut(s) 152, 332
BpuMI CCSGG 3 cut(s) 419, 897, 2249
Bsa29I ATCGAT 2 cut(s) 1141, 3264
BsaBI GATNNNNATC 3 cut(s) 491, 1119, 1143
BsaJI CCNNGG 9 cut(s) 872, 1056, 1850, 1962, 2130, 2165, 2224, 2331, 2538
BsaWI WCCGGW 3 cut(s) 147, 2800, 3220
BsaXI ACNNNNNCTCC 6 cut(s) 1813, 1843, 2243, 2273, 3107, 3137
Bsc4I CCNNNNNNNGG 9 cut(s) 425, 626, 878, 1645, 2190, 2191, 2967, 3028, 3132
Bse118I RCCGGY 1 cut(s) 3220
Bse21I CCTNAGG 1 cut(s) 612
Bse3DI GCAATG 1 cut(s) 2842
Bse8I GATNNNNATC 3 cut(s) 491, 1119, 1143
BseBI CCWGG 8 cut(s) 755, 873, 2166, 2332, 2499, 3198, 3360, 3377
BseCI ATCGAT 2 cut(s) 1141, 3264
BseDI CCNNGG 9 cut(s) 872, 1056, 1850, 1962, 2130, 2165, 2224, 2331, 2538
BseJI GATNNNNATC 3 cut(s) 491, 1119, 1143
BseLI CCNNNNNNNGG 9 cut(s) 425, 626, 878, 1645, 2190, 2191, 2967, 3028, 3132
BseMI GCAATG 1 cut(s) 2842
BseMII CTCAG 6 cut(s) 603, 959, 2417, 2667, 3201, 3380
BseRI GAGGAG 6 cut(s) 124, 612, 627, 2054, 2150, 3339
BsgI GTGCAG 3 cut(s) 225, 726, 2108
BshFI GGCC 7 cut(s) 122, 477, 589, 630, 1465, 1638, 2019
BshNI GGYRCC 1 cut(s) 658
BshTI ACCGGT 1 cut(s) 3220
BshVI ATCGAT 2 cut(s) 1141, 3264
BsiHKAI GWGCWC 5 cut(s) 1859, 2066, 2633, 3188, 3367
BsiHKCI CYCGRG 2 cut(s) 1169, 2213
BsiSI CCGG 6 cut(s) 148, 419, 896, 2249, 2801, 3221
BslFI GGGAC 6 cut(s) 2207, 2231, 2348, 2953, 2992, 3157
BslI CCNNNNNNNGG 9 cut(s) 425, 626, 878, 1645, 2190, 2191, 2967, 3028, 3132
BsmAI GTCTC 8 cut(s) 128, 873, 1348, 2132, 2590, 2650, 3095, 3274
BsmBI CGTCTC 2 cut(s) 873, 2650
BsmFI GGGAC 6 cut(s) 2207, 2231, 2348, 2953, 2992, 3157
BsmI GAATGC 2 cut(s) 203, 736
BsnI GGCC 7 cut(s) 122, 477, 589, 630, 1465, 1638, 2019
BsoBI CYCGRG 2 cut(s) 1169, 2213
Bsp119I TTCGAA 2 cut(s) 207, 1677
Bsp1286I GDGCHC 6 cut(s) 1859, 2066, 2633, 3023, 3188, 3367
Bsp1720I GCTNAGC 1 cut(s) 2426
Bsp19I CCATGG 1 cut(s) 1962
BspACI CCGC 2 cut(s) 53, 3326
BspANI GGCC 7 cut(s) 122, 477, 589, 630, 1465, 1638, 2019
BspCNI CTCAG 6 cut(s) 604, 958, 2418, 2668, 3200, 3379
BspDI ATCGAT 2 cut(s) 1141, 3264
BspHI TCATGA 2 cut(s) 646, 1618
BspLI GGNNCC 7 cut(s) 534, 618, 660, 678, 1848, 1960, 2740
BspQI GCTCTTC 1 cut(s) 3176
BspT104I TTCGAA 2 cut(s) 207, 1677
BspT107I GGYRCC 1 cut(s) 658
BsrDI GCAATG 1 cut(s) 2842
BsrFI RCCGGY 1 cut(s) 3220
BssAI RCCGGY 1 cut(s) 3220
BssECI CCNNGG 9 cut(s) 872, 1056, 1850, 1962, 2130, 2165, 2224, 2331, 2538
BssNAI GTATAC 1 cut(s) 2484
BssSI CACGAG 2 cut(s) 624, 2632
BssT1I CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
Bst1107I GTATAC 1 cut(s) 2484
Bst2BI CACGAG 2 cut(s) 624, 2632
Bst2UI CCWGG 8 cut(s) 755, 873, 2166, 2332, 2499, 3198, 3360, 3377
Bst4CI ACNGT 7 cut(s) 1212, 1359, 2033, 2068, 2481, 2859, 2992
BstAPI GCANNNNNTGC 1 cut(s) 740
BstBI TTCGAA 2 cut(s) 207, 1677
BstC8I GCNNGC 8 cut(s) 24, 51, 244, 248, 362, 976, 2055, 2755
BstDEI CTNAG 9 cut(s) 612, 945, 1904, 2303, 2426, 2431, 2676, 3187, 3366
BstDSI CCRYGG 1 cut(s) 1962
BstENI CCTNNNNNAGG 1 cut(s) 2965
BstHHI GCGC 2 cut(s) 332, 417
BstMAI GTCTC 8 cut(s) 128, 873, 1348, 2132, 2590, 2650, 3095, 3274
BstNI CCWGG 8 cut(s) 755, 873, 2166, 2332, 2499, 3198, 3360, 3377
BstNSI RCATGY 3 cut(s) 176, 442, 919
BstPAI GACNNNNGTC 1 cut(s) 865
BstSFI CTRYAG 3 cut(s) 1332, 3045, 3096
BstV2I GAAGAC 2 cut(s) 1572, 2871
BstX2I RGATCY 8 cut(s) 532, 737, 824, 1442, 1846, 2209, 2738, 2809
BstXI CCANNNNNNTGG 1 cut(s) 1986
BstYI RGATCY 8 cut(s) 532, 737, 824, 1442, 1846, 2209, 2738, 2809
BstZ17I GTATAC 1 cut(s) 2484
Bsu15I ATCGAT 2 cut(s) 1141, 3264
Bsu36I CCTNAGG 1 cut(s) 612
BsuRI GGCC 7 cut(s) 122, 477, 589, 630, 1465, 1638, 2019
BsuTUI ATCGAT 2 cut(s) 1141, 3264
BtgI CCRYGG 1 cut(s) 1962
BtgZI GCGATG 1 cut(s) 3075
BtrI CACGTC 1 cut(s) 2932
BtsI GCAGTG 1 cut(s) 1501
BtsIMutI CAGTG 7 cut(s) 1098, 1368, 1501, 2038, 2412, 2982, 3147
Cac8I GCNNGC 8 cut(s) 24, 51, 244, 248, 362, 976, 2055, 2755
CaiI CAGNNNCTG 2 cut(s) 956, 998
CciI TCATGA 2 cut(s) 646, 1618
CfoI GCGC 2 cut(s) 332, 417
Cfr10I RCCGGY 1 cut(s) 3220
Cfr13I GGNCC 8 cut(s) 121, 145, 476, 587, 629, 704, 1780, 2018
ClaI ATCGAT 2 cut(s) 1141, 3264
CseI GACGC 3 cut(s) 1998, 2348, 3361
Csp6I GTAC 4 cut(s) 1200, 1880, 2993, 3071
CspAI ACCGGT 1 cut(s) 3220
CviQI GTAC 4 cut(s) 1200, 1880, 2993, 3071
DdeI CTNAG 9 cut(s) 612, 945, 1904, 2303, 2426, 2431, 2676, 3187, 3366
DrdI GACNNNNNNGTC 2 cut(s) 1210, 3288
DseDI GACNNNNNNGTC 2 cut(s) 1210, 3288
EaeI YGGCCR 1 cut(s) 1636
Ecl136II GAGCTC 3 cut(s) 1857, 3186, 3365
Eco130I CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
Eco24I GRGCYC 4 cut(s) 1859, 3023, 3188, 3367
Eco32I GATATC 1 cut(s) 1145
Eco47I GGWCC 3 cut(s) 145, 704, 1780
Eco53kI GAGCTC 3 cut(s) 1857, 3186, 3365
Eco57I CTGAAG 9 cut(s) 308, 813, 1005, 1074, 1467, 1818, 2223, 2550, 2928
Eco81I CCTNAGG 1 cut(s) 612
Eco88I CYCGRG 2 cut(s) 1169, 2213
EcoICRI GAGCTC 3 cut(s) 1857, 3186, 3365
EcoNI CCTNNNNNAGG 1 cut(s) 2965
EcoRI GAATTC 2 cut(s) 159, 606
EcoRII CCWGG 8 cut(s) 753, 871, 2164, 2330, 2497, 3196, 3358, 3375
EcoRV GATATC 1 cut(s) 1145
EcoT14I CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
EcoT22I ATGCAT 1 cut(s) 921
EcoT38I GRGCYC 4 cut(s) 1859, 3023, 3188, 3367
ErhI CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
Esp3I CGTCTC 2 cut(s) 873, 2650
FalI AAGNNNNNCTT 6 cut(s) 1166, 1198, 1300, 1332, 2799, 2831
FaqI GGGAC 6 cut(s) 2207, 2231, 2348, 2953, 2992, 3157
FauNDI CATATG 3 cut(s) 2650, 2977, 3294
FbaI TGATCA 2 cut(s) 1501, 1615
FblI GTMKAC 4 cut(s) 1312, 2046, 2483, 3336
FriOI GRGCYC 4 cut(s) 1859, 3023, 3188, 3367
FspBI CTAG 9 cut(s) 156, 603, 666, 1458, 1839, 2703, 2853, 2862, 2904
FspI TGCGCA 1 cut(s) 331
GlaI GCGC 2 cut(s) 331, 416
HaeIII GGCC 7 cut(s) 122, 477, 589, 630, 1465, 1638, 2019
HapII CCGG 6 cut(s) 148, 419, 896, 2249, 2801, 3221
HgaI GACGC 3 cut(s) 1998, 2348, 3361
HhaI GCGC 2 cut(s) 332, 417
Hin6I GCGC 2 cut(s) 330, 415
HinP1I GCGC 2 cut(s) 330, 415
HincII GTYRAC 2 cut(s) 1660, 2442
HindII GTYRAC 2 cut(s) 1660, 2442
HindIII AAGCTT 3 cut(s) 47, 563, 2051
HpaI GTTAAC 1 cut(s) 1660
HpaII CCGG 6 cut(s) 148, 419, 896, 2249, 2801, 3221
HphI GGTGA 5 cut(s) 459, 885, 1094, 1445, 3440
Hpy166II GTNNAC 9 cut(s) 707, 1313, 1660, 1898, 2047, 2442, 2484, 2798, 3337
Hpy8I GTNNAC 9 cut(s) 707, 1313, 1660, 1898, 2047, 2442, 2484, 2798, 3337
HpyCH4III ACNGT 7 cut(s) 1212, 1359, 2033, 2068, 2481, 2859, 2992
HpyCH4IV ACGT 4 cut(s) 1227, 1871, 2768, 2931
HpyF3I CTNAG 9 cut(s) 612, 945, 1904, 2303, 2426, 2431, 2676, 3187, 3366
HpySE526I ACGT 4 cut(s) 1227, 1871, 2768, 2931
HspAI GCGC 2 cut(s) 330, 415
Ksp22I TGATCA 2 cut(s) 1501, 1615
KspAI GTTAAC 1 cut(s) 1660
LguI GCTCTTC 1 cut(s) 3176
LweI GCATC 9 cut(s) 79, 317, 326, 919, 1070, 1383, 1996, 2482, 3133
MaeI CTAG 9 cut(s) 156, 603, 666, 1458, 1839, 2703, 2853, 2862, 2904
MaeII ACGT 4 cut(s) 1227, 1871, 2768, 2931
MaeIII GTNAC 4 cut(s) 1100, 1526, 2408, 2688
MfeI CAATTG 3 cut(s) 297, 504, 2919
MflI RGATCY 8 cut(s) 532, 737, 824, 1442, 1846, 2209, 2738, 2809
MhlI GDGCHC 6 cut(s) 1859, 2066, 2633, 3023, 3188, 3367
MlyI GAGTC 7 cut(s) 269, 566, 1961, 2189, 2711, 3263, 3450
MmeI TCCRAC 2 cut(s) 807, 890
Mph1103I ATGCAT 1 cut(s) 921
MroXI GAANNNNTTC 3 cut(s) 886, 2320, 2811
MseI TTAA 8 cut(s) 44, 720, 924, 1517, 1659, 1668, 1887, 2468
MslI CAYNNNNRTG 6 cut(s) 443, 1125, 1226, 1373, 1593, 1623
MspA1I CMGCKG 3 cut(s) 953, 995, 3328
MspI CCGG 6 cut(s) 148, 419, 896, 2249, 2801, 3221
MunI CAATTG 3 cut(s) 297, 504, 2919
Mva1269I GAATGC 2 cut(s) 203, 736
MvaI CCWGG 8 cut(s) 755, 873, 2166, 2332, 2499, 3198, 3360, 3377
NciI CCSGG 3 cut(s) 419, 897, 2249
NcoI CCATGG 1 cut(s) 1962
NdeI CATATG 3 cut(s) 2650, 2977, 3294
NlaIV GGNNCC 7 cut(s) 534, 618, 660, 678, 1848, 1960, 2740
NmuCI GTSAC 4 cut(s) 1100, 1526, 2408, 2688
NsbI TGCGCA 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 3 cut(s) 176, 442, 919
NspV TTCGAA 2 cut(s) 207, 1677
OliI CACNNNNGTG 2 cut(s) 1226, 1373
PagI TCATGA 2 cut(s) 646, 1618
PciI ACATGT 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 3176
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 2 cut(s) 203, 736
PdmI GAANNNNTTC 3 cut(s) 886, 2320, 2811
PfoI TCCNGGA 4 cut(s) 753, 2247, 3196, 3375
PinAI ACCGGT 1 cut(s) 3220
PleI GAGTC 7 cut(s) 268, 565, 1961, 2189, 2711, 3262, 3449
PpsI GAGTC 7 cut(s) 268, 565, 1961, 2189, 2711, 3262, 3449
PscI ACATGT 1 cut(s) 172
PshAI GACNNNNGTC 1 cut(s) 865
Psp124BI GAGCTC 3 cut(s) 1859, 3188, 3367
Psp6I CCWGG 8 cut(s) 753, 871, 2164, 2330, 2497, 3196, 3358, 3375
PspGI CCWGG 8 cut(s) 753, 871, 2164, 2330, 2497, 3196, 3358, 3375
PspN4I GGNNCC 7 cut(s) 534, 618, 660, 678, 1848, 1960, 2740
PspPI GGNCC 8 cut(s) 121, 145, 476, 587, 629, 704, 1780, 2018
PstNI CAGNNNCTG 2 cut(s) 956, 998
PsuI RGATCY 8 cut(s) 532, 737, 824, 1442, 1846, 2209, 2738, 2809
PvuII CAGCTG 2 cut(s) 953, 995
RsaI GTAC 4 cut(s) 1201, 1881, 2994, 3072
RsaNI GTAC 4 cut(s) 1200, 1880, 2993, 3071
RseI CAYNNNNRTG 6 cut(s) 443, 1125, 1226, 1373, 1593, 1623
SacI GAGCTC 3 cut(s) 1859, 3188, 3367
SapI GCTCTTC 1 cut(s) 3176
SaqAI TTAA 8 cut(s) 44, 720, 924, 1517, 1659, 1668, 1887, 2468
Sau96I GGNCC 8 cut(s) 121, 145, 476, 587, 629, 704, 1780, 2018
SchI GAGTC 7 cut(s) 269, 566, 1961, 2189, 2711, 3263, 3450
SduI GDGCHC 6 cut(s) 1859, 2066, 2633, 3023, 3188, 3367
SfaNI GCATC 9 cut(s) 79, 317, 326, 919, 1070, 1383, 1996, 2482, 3133
SfcI CTRYAG 3 cut(s) 1332, 3045, 3096
SfuI TTCGAA 2 cut(s) 207, 1677
SinI GGWCC 3 cut(s) 145, 704, 1780
SmiMI CAYNNNNRTG 6 cut(s) 443, 1125, 1226, 1373, 1593, 1623
SmlI CTYRAG 2 cut(s) 131, 347
SmoI CTYRAG 2 cut(s) 131, 347
SsiI CCGC 2 cut(s) 53, 3326
SspMI CTAG 9 cut(s) 156, 603, 666, 1458, 1839, 2703, 2853, 2862, 2904
SstI GAGCTC 3 cut(s) 1859, 3188, 3367
StyI CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
TaaI ACNGT 7 cut(s) 1212, 1359, 2033, 2068, 2481, 2859, 2992
TaiI ACGT 4 cut(s) 1230, 1874, 2771, 2934
TaqI TCGA 7 cut(s) 207, 216, 1117, 1141, 1677, 3264, 3429
TatI WGTACW 2 cut(s) 1879, 2992
TauI GCSGC 1 cut(s) 3328
Tru1I TTAA 8 cut(s) 44, 720, 924, 1517, 1659, 1668, 1887, 2468
Tru9I TTAA 8 cut(s) 44, 720, 924, 1517, 1659, 1668, 1887, 2468
TscAI CASTG 7 cut(s) 1098, 1375, 1501, 2038, 2419, 2989, 3154
TseFI GTSAC 4 cut(s) 1100, 1526, 2408, 2688
Tsp45I GTSAC 4 cut(s) 1100, 1526, 2408, 2688
TspGWI ACGGA 4 cut(s) 76, 1537, 2641, 2709
TspRI CASTG 7 cut(s) 1098, 1375, 1501, 2038, 2419, 2989, 3154
VpaK11BI GGWCC 3 cut(s) 145, 704, 1780
XagI CCTNNNNNAGG 1 cut(s) 2965
XbaI TCTAGA 1 cut(s) 2861
XceI RCATGY 3 cut(s) 176, 442, 919
XcmI CCANNNNNNNNNTGG 1 cut(s) 3030
XmiI GTMKAC 4 cut(s) 1312, 2046, 2483, 3336
XmnI GAANNNNTTC 3 cut(s) 886, 2320, 2811
XspI CTAG 9 cut(s) 156, 603, 666, 1458, 1839, 2703, 2853, 2862, 2904
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.