MD15G1202900.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008827
Physical Location & Seq
Reverse (-)
16132241 .. 16138070
5830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1202900.v1.1.491

Sequence Viewer

Length: 3822 bp
ATGCTTCACAGGAGCTTCAAGCCTGCCAAATGCAAAACGGCATTAAAGCTCGCGGTTTCGCGGATAAAGCTATTGAAGAACAAGAAGGATGCACAAGTGAAGCAGATGAGGAGGGAATTGGCCCAGTTGCTTGAGACTGGTCAATACCGGACTGCGAGAATTCGGGTTGAACATGTGGTCAGAGAAGAGAAGACAAAGGCAGCGTTTGAACTTATCGAGATATATTGCGAACTTATTGCTGCACGCTTGCCTATGATTGAGTCACAAAAAAACTGCCCCATTGATCTGAAGGAAGCAATTACAAGTGTAGTATTTGCATCTCCAAGATGCGCAGACATACCAGAACTCATGGATATTCGCAAGCATTTCACAGCTAAATATGGAAAGGAGTTTATCTCTAACGCTGTTGAATTGCGTCCAGATTGTGGCGTAAGTCGCATGTTAGTGGAGAAATTATCTACCAAATCACCTGATGGCCCTACCAAGATGAAAATCTTAGCTGCAATTGCAGAGGAACATAATGTCAAATGGGATCCTGAATCCTTTGAAGAGAAAGAGTCCAAGCCTCCAGAGGACCTGCTGAATGGGCCAAATACTTTTGCTAGAATTCCTGAGGAACCTCCTCGTGGCCCACTTCCTCCTAATCATGATGTCAAGGCACCTTCTAGTGTTCAGGCTCCACCTCAAAATTATGGGAATTATGGTCCACCCACGAACTTTAACGAGCTGAATGCAAGATCTGCACCTCGCTTCCAGGATTCTGCTTCTTCAAATGTTTCTTCCAACAAAGCAACTACATCTGGCTCATTTCATTCAGAAGTGAGACCTTCAGGAAATCAAAATGATGGGATGGAAAATAGACATTCGTCTCCTGAGGATGGAAATTCTTCTTCACCGGGTAGGCAGAGTTGGAACATGCATTTTAAGGATGCTGCTTCTGCAGCACAGGCAGCTGCTGAATCAGCCGAAATGGCAGGCATGGCTGCCAGAGCAGCTGTTGAACTTTCAAGAAAACACTATTCAGAATCACAGGATAAATTTAGCCAGTCAGCTCCCTTGGCATCTGATAGAACATCCACAAATGATCCTTCGGTGAATAGGCACCAAATGCAAGATCGACATCCTGACGAAGTGTCAGATCGATATCCTCAAAAGAAGTCATCTGAACCTGAGAAGCATGACTTTGTAAGTGAAGTGAGTACGACAAGACAGTCCAGCAACACTAACGTGTATGCAAGTGAGGTGCAAACTGGAATGAAACTTGATAAAACTTATTATTTGGGTAATATGAGATCAGAAGAGAAATCAAGTAGACCTTCCCAATCCAATCCTATAGGTGAAGTGAGTGTGAAGAGACAGTCGAGCAACACTGGTGTGGAGTATGGGAGTGAGATGCAAACTGGAAACAAACCTGATAACGTTGCTTATTTTGGTGATATGAGATCTGAAGAGAAATCTAGTAGGCCTTCCTCCCGATCTAATTCAAGCATTGGCAGTGATGATCAGGAAGATGTTTTCAGACGGAGTGACGATATTTCTTATTTGGGAAATAAGAGGAGTGGAGAACAATCTATCTGGTCTTCCTGTCATTCTCATTCAAGAAATTTTAGTAATGATCATGAAAGTGTTTCAAATGGCCGTGATGAGGATTCTTTTGTTAACGATGTTAATATCTTCCAAAGCACTAAGGAAATATGTTCTGATGGAAATGCTCCTTTGGTTTTTGATGAATCTGGTTCAGACGACGATAAATGTAAATTTGATGTGGAGGACTACAAGGGCCAAGAACCCAGTTTGTTTTTTCCTTTTCCTGATAGAAACTCATTTTCTCCTTCAGCTAGTTTGTATCCTTGGAGCTCTAAACAACAGACGTATGAAGTACAATTTAAGTCCAGTTCACAACTAAGTTCTTCATCGGTGAAGCACTCCCCTCCTGTTTTTTCAGAAAATTCAACAGGTTCCATGGACTCTTCACAACCAAATGACTTGTTGCCTGTTGCTTTTGATGCGTCAGATGGCCCAAGTTCAGACAGTGAAGAGGAGGTAGACAAGCTTGCCAAGAGCACAGTTTCTAAATTTTCCCTTGTGCAGAGCCAAAGTGCAAGTCGTAGGTCGTTTGGATCTTCATCCTCGGAGGAGATAAATATAGGTTCTAACACAAAGCCAGGGTTGCTGCTTTCATCTCATAATTTGGACTCAACTGAAGCACATCCCCAGAGAAGCCAAGGAGACAAAAATAGAATCGTCCCGGAGGAAAAGTATGATTTTGATGAAGTGCCTACTGGTCAGCCATCTGTAAGGCTAAGGAAATCTGGATTTGATTCAAATGCCAGGGACGATTTTCAAACACAACATTTGCATCAAACTATGAAAGATAGTGAGGTTTCAGATGGATGTAGTTGTGTCAGTGACACTGACAATGAGCTGAGCTTAGGGATGTTGACAGGTGGGCTGCGAAATAAGGGTTTAAGGCATCCACCGTATACGAAGAAATTACCAGGAAACTTTTTGTCTGTTAAACAAGATACTGAAGACACCAAGGTTGAGCAACCTTCCTTCTTTCCATCAGTCAGGACTTCTGTTGTCTCTGAAGGAGAGCAAGGGAGCAAAAAACTGATCAAGGAATGGAGCACGAGAACCCCCATCTCATATGTGGCTTCTGATGATGATTCTTCCGAGGATGAATTGTCACACAGAACTGCTAGAAGTAGCCAAGACTCATTCAATAAAAAATTGGGATCTGAAGTAAAGGCTAGCTCCAAATCCACGTTTTCTTTCTTTGATTCAGAGGATAGTGAGCCGGAAGAAGATCTTCTTACAAAGATTTCCACCAGCAATGCTCGTCCAAGTGCTAGACTGTCTAGAAGAACACAGCTGTCTTCTTCGAATTCTGTTAGAAGCTCTAGCTCAAAGACAACAGTAGTTTCTGACTTGTCAGGGACTTCAGAGTATGGAAAACCCTCTTCAAGGAGTTCATATGACACTGAAACTGTAGTAAAGCCCTCGTCCCAGATCAAGAGCTCAGAGCAGGAGTGGAATAGACCTGTAGAACAAGTTGCTCCTAAACCAGTACGAGAATCCAAGAGGACATCACCTGTAGAGACTTCAAATTCAAACAGAAACTCCATTTCAAGGAGTTCCGATGCCACTGAAACTTTACCAAAGTCCTTGTCCCAGGCGAAGAGTTCAGATAGTCCAGGAAGTCAAGAGTGGCATAAACCAGTAGAACAAGCAGCTCCTAAACCAATGCCAGAGTCCAAGAGATCCTCACTTGTGGAGACTTCAAAGTCATATGGAAACCCCTCATCAAGGAGTTCCTATGCCGCTGAAAGTCTACCAAAGCCCTCCTCCCAGACCAGGAGCTCAGAGCATCCAGGAAGTCAGGAGTGGCATAGACCAGCAGAACAAGCAGCTCCTAAACCAGTTCGAGAATCCAAGAGTCCTTCACCTGAAGATGTAGAGACTTCTAAGTCATATGGAAACCCTTCTTCAAGAAGTGCGTATGCTTCTGAGACCCTACCAAATCCCTCGTCACAGACCAAGAGTTCAGAGCACCCAAGAAGACAGGATCGACAAAGACAAGTAGAACAAGCAGCGCCTAAACCAGTTTTGGAATCCAAGAGGCCCTCCTCACATGTAGAGACCTCAAAGCCATTTGCAAGGGAGCAATCATCCGAAAATTCTCAAAAGATCGAAACATCAAAGTCCTCAGGTTCTACAGAGAATCCACCAAAGGAGAAGGTTTCTCATGTTCACCCAAAGCTTCCCAACTCTGATGACATAATCGCCAGGTTTATGGCTCTCCGGCAGAATCGACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1274

Amino Acids

140.66

Weight (kDa)

6.3

Isoelectric Point (pI)

69.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 3.4e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 1210, 3286, 3469
Acc16I TGCGCA 1 cut(s) 331
Acc36I ACCTGC 1 cut(s) 585
AccB1I GGYRCC 2 cut(s) 658, 1101
AccB7I CCANNNNNTGG 1 cut(s) 425
AccI GTMKAC 4 cut(s) 1312, 2046, 2483, 3334
AccII CGCG 2 cut(s) 53, 61
AciI CCGC 3 cut(s) 53, 61, 3324
AclI AACGTT 1 cut(s) 1419
AclWI GGATC 7 cut(s) 527, 540, 1079, 2128, 2746, 3258, 3576
AcoI YGGCCR 1 cut(s) 1636
AfaI GTAC 3 cut(s) 1201, 1881, 3072
AfiI CCNNNNNNNGG 8 cut(s) 425, 626, 878, 1645, 2967, 3132, 3309, 3357
AflIII ACRYGT 3 cut(s) 172, 1227, 3634
AhdI GACNNNNNGTC 1 cut(s) 1132
AjnI CCWGG 9 cut(s) 753, 2164, 2330, 2497, 3174, 3196, 3356, 3373, 3788
AleI CACNNNNGTG 2 cut(s) 1226, 1373
Alw21I GWGCWC 6 cut(s) 1859, 2066, 2633, 3023, 3365, 3555
AlwI GGATC 7 cut(s) 527, 540, 1079, 2128, 2746, 3258, 3576
AlwNI CAGNNNCTG 1 cut(s) 956
AoxI GGCC 9 cut(s) 120, 475, 587, 628, 1463, 1636, 1780, 2017, 3623
ArsI GACNNNNNNTTYG 6 cut(s) 2089, 2121, 3155, 3187, 3515, 3547
Asp700I GAANNNNTTC 3 cut(s) 886, 2811, 3484
AspLEI GCGC 2 cut(s) 332, 3598
AspS9I GGNCC 9 cut(s) 121, 476, 574, 587, 629, 704, 1780, 2018, 3624
AsuC2I CCSGG 2 cut(s) 897, 2249
AsuHPI GGTGA 9 cut(s) 459, 885, 1105, 1349, 1445, 1930, 3084, 3438, 3746
AsuII TTCGAA 1 cut(s) 2885
AsuNHI GCTAGC 1 cut(s) 2753
AvaII GGWCC 2 cut(s) 574, 704
AxyI CCTNAGG 3 cut(s) 612, 873, 3709
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 2 cut(s) 658, 1101
BanII GRGCYC 3 cut(s) 1859, 3023, 3365
BauI CACGAG 2 cut(s) 624, 2632
BbsI GAAGAC 5 cut(s) 197, 1572, 2538, 2871, 3568
Bbv12I GWGCWC 6 cut(s) 1859, 2066, 2633, 3023, 3365, 3555
BceAI ACGGC 2 cut(s) 54, 1623
BcgI CGANNNNNNTGC 4 cut(s) 218, 252, 713, 747
BciT130I CCWGG 9 cut(s) 755, 2166, 2332, 2499, 3176, 3198, 3358, 3375, 3790
BciVI GTATCC 1 cut(s) 1857
BclI TGATCA 3 cut(s) 1501, 1615, 2616
BcnI CCSGG 2 cut(s) 897, 2249
BfaI CTAG 9 cut(s) 603, 666, 1458, 1839, 2703, 2754, 2853, 2862, 2904
BfmI CTRYAG 6 cut(s) 939, 1332, 2991, 3045, 3096, 3717
BfoI RGCGCY 1 cut(s) 3599
BfuAI ACCTGC 1 cut(s) 585
BfuI GTATCC 1 cut(s) 1857
BglI GCCNNNNNGGC 1 cut(s) 971
BglII AGATCT 3 cut(s) 737, 1442, 2809
BlpI GCTNAGC 1 cut(s) 2426
Bme18I GGWCC 2 cut(s) 574, 704
BmeRI GACNNNNNGTC 1 cut(s) 1132
BmgT120I GGNCC 9 cut(s) 121, 476, 574, 587, 629, 704, 1780, 2018, 3624
BmiI GGNNCC 6 cut(s) 534, 618, 660, 678, 1103, 1960
BmrI ACTGGG 2 cut(s) 118, 1785
BmtI GCTAGC 1 cut(s) 2757
BmuI ACTGGG 2 cut(s) 118, 1785
BoxI GACNNNNGTC 1 cut(s) 865
BpiI GAAGAC 5 cut(s) 197, 1572, 2538, 2871, 3568
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 1 cut(s) 552
Bpu10I CCTNAGC 2 cut(s) 2303, 2431
Bpu1102I GCTNAGC 1 cut(s) 2426
Bpu14I TTCGAA 1 cut(s) 2885
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 2 cut(s) 897, 2249
Bsa29I ATCGAT 1 cut(s) 1141
BsaBI GATNNNNATC 4 cut(s) 491, 1119, 1143, 2125
BsaI GGTCTC 3 cut(s) 817, 3506, 3635
BsaWI WCCGGW 1 cut(s) 147
BsaXI ACNNNNNCTCC 4 cut(s) 2243, 2273, 3107, 3137
Bsc4I CCNNNNNNNGG 8 cut(s) 425, 626, 878, 1645, 2967, 3132, 3309, 3357
Bse21I CCTNAGG 3 cut(s) 612, 873, 3709
Bse3DI GCAATG 1 cut(s) 2842
Bse8I GATNNNNATC 4 cut(s) 491, 1119, 1143, 2125
BseBI CCWGG 9 cut(s) 755, 2166, 2332, 2499, 3176, 3198, 3358, 3375, 3790
BseCI ATCGAT 1 cut(s) 1141
BseJI GATNNNNATC 4 cut(s) 491, 1119, 1143, 2125
BseLI CCNNNNNNNGG 8 cut(s) 425, 626, 878, 1645, 2967, 3132, 3309, 3357
BseMI GCAATG 1 cut(s) 2842
BseMII CTCAG 8 cut(s) 603, 864, 1161, 2417, 3036, 3378, 3501, 3723
BseRI GAGGAG 7 cut(s) 124, 612, 1570, 2054, 2150, 3337, 3619
BsgI GTGCAG 3 cut(s) 225, 726, 2108
Bsh1236I CGCG 2 cut(s) 53, 61
BshFI GGCC 9 cut(s) 122, 477, 589, 630, 1465, 1638, 1782, 2019, 3625
BshNI GGYRCC 2 cut(s) 658, 1101
BshVI ATCGAT 1 cut(s) 1141
BsiHKAI GWGCWC 6 cut(s) 1859, 2066, 2633, 3023, 3365, 3555
BsiSI CCGG 5 cut(s) 148, 896, 2249, 2801, 3805
BslFI GGGAC 5 cut(s) 2231, 2348, 2953, 2992, 3157
BslI CCNNNNNNNGG 8 cut(s) 425, 626, 878, 1645, 2967, 3132, 3309, 3357
BsmBI CGTCTC 1 cut(s) 873
BsmFI GGGAC 5 cut(s) 2231, 2348, 2953, 2992, 3157
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 9 cut(s) 122, 477, 589, 630, 1465, 1638, 1782, 2019, 3625
Bso31I GGTCTC 3 cut(s) 817, 3506, 3635
Bsp119I TTCGAA 1 cut(s) 2885
Bsp1286I GDGCHC 6 cut(s) 1859, 2066, 2633, 3023, 3365, 3555
Bsp1720I GCTNAGC 1 cut(s) 2426
Bsp19I CCATGG 1 cut(s) 1962
BspACI CCGC 3 cut(s) 53, 61, 3324
BspANI GGCC 9 cut(s) 122, 477, 589, 630, 1465, 1638, 1782, 2019, 3625
BspCNI CTCAG 8 cut(s) 604, 865, 1162, 2418, 3035, 3377, 3502, 3722
BspDI ATCGAT 1 cut(s) 1141
BspFNI CGCG 2 cut(s) 53, 61
BspHI TCATGA 2 cut(s) 646, 1618
BspLI GGNNCC 6 cut(s) 534, 618, 660, 678, 1103, 1960
BspMAI CTGCAG 1 cut(s) 943
BspMI ACCTGC 1 cut(s) 585
BspOI GCTAGC 1 cut(s) 2757
BspPI GGATC 7 cut(s) 527, 540, 1079, 2128, 2746, 3258, 3576
BspT104I TTCGAA 1 cut(s) 2885
BspT107I GGYRCC 2 cut(s) 658, 1101
BspTNI GGTCTC 3 cut(s) 817, 3506, 3635
BsrDI GCAATG 1 cut(s) 2842
BssNAI GTATAC 1 cut(s) 2484
BssSI CACGAG 2 cut(s) 624, 2632
BssT1I CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
Bst1107I GTATAC 1 cut(s) 2484
Bst2BI CACGAG 2 cut(s) 624, 2632
Bst2UI CCWGG 9 cut(s) 755, 2166, 2332, 2499, 3176, 3198, 3358, 3375, 3790
Bst4CI ACNGT 9 cut(s) 1212, 1359, 2033, 2068, 2481, 2859, 2920, 2992, 3819
Bst6I CTCTTC 9 cut(s) 180, 543, 1293, 1346, 1443, 1975, 2031, 2968, 3176
BstAPI GCANNNNNTGC 2 cut(s) 740, 1108
BstBI TTCGAA 1 cut(s) 2885
BstC8I GCNNGC 8 cut(s) 24, 51, 244, 248, 362, 976, 2055, 2755
BstDSI CCRYGG 1 cut(s) 1962
BstENI CCTNNNNNAGG 2 cut(s) 2965, 3307
BstFNI CGCG 2 cut(s) 53, 61
BstH2I RGCGCY 1 cut(s) 3599
BstHHI GCGC 2 cut(s) 332, 3598
BstNI CCWGG 9 cut(s) 755, 2166, 2332, 2499, 3176, 3198, 3358, 3375, 3790
BstNSI RCATGY 4 cut(s) 176, 442, 919, 3638
BstPAI GACNNNNGTC 1 cut(s) 865
BstSFI CTRYAG 6 cut(s) 939, 1332, 2991, 3045, 3096, 3717
BstUI CGCG 2 cut(s) 53, 61
BstV2I GAAGAC 5 cut(s) 197, 1572, 2538, 2871, 3568
BstX2I RGATCY 7 cut(s) 532, 737, 1442, 2120, 2738, 2809, 3263
BstXI CCANNNNNNTGG 1 cut(s) 3796
BstYI RGATCY 7 cut(s) 532, 737, 1442, 2120, 2738, 2809, 3263
BstZ17I GTATAC 1 cut(s) 2484
Bsu15I ATCGAT 1 cut(s) 1141
Bsu36I CCTNAGG 3 cut(s) 612, 873, 3709
BsuI GTATCC 1 cut(s) 1857
BsuRI GGCC 9 cut(s) 122, 477, 589, 630, 1465, 1638, 1782, 2019, 3625
BsuTUI ATCGAT 1 cut(s) 1141
BtgI CCRYGG 1 cut(s) 1962
BtsI GCAGTG 1 cut(s) 1501
BtsIMutI CAGTG 7 cut(s) 1368, 1501, 2038, 2412, 2413, 2982, 3147
BveI ACCTGC 1 cut(s) 585
Cac8I GCNNGC 8 cut(s) 24, 51, 244, 248, 362, 976, 2055, 2755
CaiI CAGNNNCTG 1 cut(s) 956
CciI TCATGA 2 cut(s) 646, 1618
CfoI GCGC 2 cut(s) 332, 3598
Cfr13I GGNCC 9 cut(s) 121, 476, 574, 587, 629, 704, 1780, 2018, 3624
ClaI ATCGAT 1 cut(s) 1141
CseI GACGC 2 cut(s) 404, 1998
Csp6I GTAC 3 cut(s) 1200, 1880, 3071
CviQI GTAC 3 cut(s) 1200, 1880, 3071
DrdI GACNNNNNNGTC 3 cut(s) 1210, 3286, 3469
DriI GACNNNNNGTC 1 cut(s) 1132
DseDI GACNNNNNNGTC 3 cut(s) 1210, 3286, 3469
EaeI YGGCCR 1 cut(s) 1636
Eam1104I CTCTTC 9 cut(s) 180, 543, 1293, 1346, 1443, 1975, 2031, 2968, 3176
Eam1105I GACNNNNNGTC 1 cut(s) 1132
EarI CTCTTC 9 cut(s) 180, 543, 1293, 1346, 1443, 1975, 2031, 2968, 3176
Ecl136II GAGCTC 3 cut(s) 1857, 3021, 3363
Eco130I CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
Eco147I AGGCCT 1 cut(s) 1465
Eco24I GRGCYC 3 cut(s) 1859, 3023, 3365
Eco31I GGTCTC 3 cut(s) 817, 3506, 3635
Eco32I GATATC 1 cut(s) 1145
Eco47I GGWCC 2 cut(s) 574, 704
Eco53kI GAGCTC 3 cut(s) 1857, 3021, 3363
Eco81I CCTNAGG 3 cut(s) 612, 873, 3709
EcoICRI GAGCTC 3 cut(s) 1857, 3021, 3363
EcoNI CCTNNNNNAGG 2 cut(s) 2965, 3307
EcoO109I RGGNCCY 2 cut(s) 574, 3624
EcoRI GAATTC 3 cut(s) 159, 606, 2887
EcoRII CCWGG 9 cut(s) 753, 2164, 2330, 2497, 3174, 3196, 3356, 3373, 3788
EcoRV GATATC 1 cut(s) 1145
EcoT14I CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
EcoT22I ATGCAT 1 cut(s) 921
EcoT38I GRGCYC 3 cut(s) 1859, 3023, 3365
ErhI CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
Esp3I CGTCTC 1 cut(s) 873
FalI AAGNNNNNCTT 8 cut(s) 1166, 1198, 1300, 1332, 2796, 2828, 2799, 2831
FaqI GGGAC 5 cut(s) 2231, 2348, 2953, 2992, 3157
FauNDI CATATG 4 cut(s) 2650, 2977, 3292, 3475
FbaI TGATCA 3 cut(s) 1501, 1615, 2616
FblI GTMKAC 4 cut(s) 1312, 2046, 2483, 3334
FriOI GRGCYC 3 cut(s) 1859, 3023, 3365
FspBI CTAG 9 cut(s) 603, 666, 1458, 1839, 2703, 2754, 2853, 2862, 2904
FspI TGCGCA 1 cut(s) 331
GlaI GCGC 2 cut(s) 331, 3597
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 1 cut(s) 3599
HaeIII GGCC 9 cut(s) 122, 477, 589, 630, 1465, 1638, 1782, 2019, 3625
HapII CCGG 5 cut(s) 148, 896, 2249, 2801, 3805
HgaI GACGC 2 cut(s) 404, 1998
HhaI GCGC 2 cut(s) 332, 3598
Hin6I GCGC 2 cut(s) 330, 3596
HinP1I GCGC 2 cut(s) 330, 3596
HincII GTYRAC 2 cut(s) 1660, 2442
HindII GTYRAC 2 cut(s) 1660, 2442
HindIII AAGCTT 2 cut(s) 2051, 3761
HpaI GTTAAC 1 cut(s) 1660
HpaII CCGG 5 cut(s) 148, 896, 2249, 2801, 3805
HphI GGTGA 9 cut(s) 459, 885, 1105, 1349, 1445, 1930, 3084, 3438, 3746
Hpy166II GTNNAC 9 cut(s) 707, 1313, 1660, 1898, 2047, 2442, 2484, 3335, 3754
Hpy8I GTNNAC 9 cut(s) 707, 1313, 1660, 1898, 2047, 2442, 2484, 3335, 3754
Hpy99I CGWCG 1 cut(s) 1748
HpyCH4III ACNGT 9 cut(s) 1212, 1359, 2033, 2068, 2481, 2859, 2920, 2992, 3819
HpyCH4IV ACGT 4 cut(s) 1227, 1419, 1871, 2768
HpySE526I ACGT 4 cut(s) 1227, 1419, 1871, 2768
HspAI GCGC 2 cut(s) 330, 3596
Ksp22I TGATCA 3 cut(s) 1501, 1615, 2616
KspAI GTTAAC 1 cut(s) 1660
MaeI CTAG 9 cut(s) 603, 666, 1458, 1839, 2703, 2754, 2853, 2862, 2904
MaeII ACGT 4 cut(s) 1227, 1419, 1871, 2768
MaeIII GTNAC 5 cut(s) 261, 1526, 2408, 2688, 3531
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 7 cut(s) 532, 737, 1442, 2120, 2738, 2809, 3263
MhlI GDGCHC 6 cut(s) 1859, 2066, 2633, 3023, 3365, 3555
MlyI GAGTC 7 cut(s) 269, 566, 1961, 2189, 2711, 3263, 3448
MmeI TCCRAC 2 cut(s) 807, 890
Mph1103I ATGCAT 1 cut(s) 921
MroXI GAANNNNTTC 3 cut(s) 886, 2811, 3484
MseI TTAA 8 cut(s) 44, 720, 924, 1659, 1668, 1887, 2468, 2517
MslI CAYNNNNRTG 4 cut(s) 443, 1226, 1373, 1623
MspA1I CMGCKG 4 cut(s) 953, 995, 2875, 3326
MspI CCGG 5 cut(s) 148, 896, 2249, 2801, 3805
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 9 cut(s) 755, 2166, 2332, 2499, 3176, 3198, 3358, 3375, 3790
MvnI CGCG 2 cut(s) 53, 61
NciI CCSGG 2 cut(s) 897, 2249
NcoI CCATGG 1 cut(s) 1962
NdeI CATATG 4 cut(s) 2650, 2977, 3292, 3475
NheI GCTAGC 1 cut(s) 2753
NlaIV GGNNCC 6 cut(s) 534, 618, 660, 678, 1103, 1960
NmuCI GTSAC 5 cut(s) 261, 1526, 2408, 2688, 3531
NsbI TGCGCA 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 4 cut(s) 176, 442, 919, 3638
NspV TTCGAA 1 cut(s) 2885
OliI CACNNNNGTG 2 cut(s) 1226, 1373
PagI TCATGA 2 cut(s) 646, 1618
PceI AGGCCT 1 cut(s) 1465
PciI ACATGT 2 cut(s) 172, 3634
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 3 cut(s) 886, 2811, 3484
PflMI CCANNNNNTGG 1 cut(s) 425
PfoI TCCNGGA 4 cut(s) 753, 2247, 3196, 3373
PleI GAGTC 7 cut(s) 268, 565, 1961, 2189, 2711, 3262, 3447
PpsI GAGTC 7 cut(s) 268, 565, 1961, 2189, 2711, 3262, 3447
PpuMI RGGWCCY 1 cut(s) 574
PscI ACATGT 2 cut(s) 172, 3634
PshAI GACNNNNGTC 1 cut(s) 865
Psp124BI GAGCTC 3 cut(s) 1859, 3023, 3365
Psp1406I AACGTT 1 cut(s) 1419
Psp5II RGGWCCY 1 cut(s) 574
Psp6I CCWGG 9 cut(s) 753, 2164, 2330, 2497, 3174, 3196, 3356, 3373, 3788
PspGI CCWGG 9 cut(s) 753, 2164, 2330, 2497, 3174, 3196, 3356, 3373, 3788
PspN4I GGNNCC 6 cut(s) 534, 618, 660, 678, 1103, 1960
PspPI GGNCC 9 cut(s) 121, 476, 574, 587, 629, 704, 1780, 2018, 3624
PspPPI RGGWCCY 1 cut(s) 574
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 7 cut(s) 532, 737, 1442, 2120, 2738, 2809, 3263
PvuII CAGCTG 3 cut(s) 953, 995, 2875
RsaI GTAC 3 cut(s) 1201, 1881, 3072
RsaNI GTAC 3 cut(s) 1200, 1880, 3071
RseI CAYNNNNRTG 4 cut(s) 443, 1226, 1373, 1623
SacI GAGCTC 3 cut(s) 1859, 3023, 3365
SaqAI TTAA 8 cut(s) 44, 720, 924, 1659, 1668, 1887, 2468, 2517
Sau96I GGNCC 9 cut(s) 121, 476, 574, 587, 629, 704, 1780, 2018, 3624
SchI GAGTC 7 cut(s) 269, 566, 1961, 2189, 2711, 3263, 3448
SduI GDGCHC 6 cut(s) 1859, 2066, 2633, 3023, 3365, 3555
SfcI CTRYAG 6 cut(s) 939, 1332, 2991, 3045, 3096, 3717
SfuI TTCGAA 1 cut(s) 2885
SinI GGWCC 2 cut(s) 574, 704
SmiMI CAYNNNNRTG 4 cut(s) 443, 1226, 1373, 1623
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SseBI AGGCCT 1 cut(s) 1465
SsiI CCGC 3 cut(s) 53, 61, 3324
SspMI CTAG 9 cut(s) 603, 666, 1458, 1839, 2703, 2754, 2853, 2862, 2904
SstI GAGCTC 3 cut(s) 1859, 3023, 3365
StuI AGGCCT 1 cut(s) 1465
StyI CCWWGG 5 cut(s) 1056, 1850, 1962, 2224, 2538
TaaI ACNGT 9 cut(s) 1212, 1359, 2033, 2068, 2481, 2859, 2920, 2992, 3819
TaiI ACGT 4 cut(s) 1230, 1422, 1874, 2771
TaqI TCGA 9 cut(s) 216, 1117, 1141, 1361, 2885, 3427, 3571, 3693, 3814
TatI WGTACW 1 cut(s) 1879
TauI GCSGC 1 cut(s) 3326
Tru1I TTAA 8 cut(s) 44, 720, 924, 1659, 1668, 1887, 2468, 2517
Tru9I TTAA 8 cut(s) 44, 720, 924, 1659, 1668, 1887, 2468, 2517
TscAI CASTG 7 cut(s) 1375, 1501, 2038, 2413, 2419, 2989, 3154
TseFI GTSAC 5 cut(s) 261, 1526, 2408, 2688, 3531
Tsp45I GTSAC 5 cut(s) 261, 1526, 2408, 2688, 3531
TspGWI ACGGA 1 cut(s) 1537
TspRI CASTG 7 cut(s) 1375, 1501, 2038, 2413, 2419, 2989, 3154
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 574, 704
XagI CCTNNNNNAGG 2 cut(s) 2965, 3307
XbaI TCTAGA 1 cut(s) 2861
XceI RCATGY 4 cut(s) 176, 442, 919, 3638
XcmI CCANNNNNNNNNTGG 1 cut(s) 2650
XmiI GTMKAC 4 cut(s) 1312, 2046, 2483, 3334
XmnI GAANNNNTTC 3 cut(s) 886, 2811, 3484
XspI CTAG 9 cut(s) 603, 666, 1458, 1839, 2703, 2754, 2853, 2862, 2904
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.